CN110088284A - 高水平蒂巴因罂粟及其生产的方法 - Google Patents

高水平蒂巴因罂粟及其生产的方法 Download PDF

Info

Publication number
CN110088284A
CN110088284A CN201780063464.9A CN201780063464A CN110088284A CN 110088284 A CN110088284 A CN 110088284A CN 201780063464 A CN201780063464 A CN 201780063464A CN 110088284 A CN110088284 A CN 110088284A
Authority
CN
China
Prior art keywords
endogenous gene
t6odm
coding
codm
plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201780063464.9A
Other languages
English (en)
Chinese (zh)
Inventor
伊戈尔·科瓦楚克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Api Laboratory Co Ltd
Original Assignee
Api Laboratory Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Api Laboratory Co Ltd filed Critical Api Laboratory Co Ltd
Publication of CN110088284A publication Critical patent/CN110088284A/zh
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47—Quinolines; Isoquinolines
    • A61K31/485—Morphinan derivatives, e.g. morphine, codeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/04—Centrally acting analgesics, e.g. opioids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C12Y114/11031—Thebaine 6-O-demethylase (1.14.11.31)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C12Y114/11032—Codeine 3-O-demethylase (1.14.11.32)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nutrition Science (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pain & Pain Management (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Emergency Medicine (AREA)
  • Epidemiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201780063464.9A 2016-08-12 2017-08-11 高水平蒂巴因罂粟及其生产的方法 Pending CN110088284A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662374682P 2016-08-12 2016-08-12
US62/374,682 2016-08-12
CA2,941,315 2016-09-07
CA2941315A CA2941315C (en) 2016-08-12 2016-09-07 High thebaine poppy and methods of producing the same
PCT/CA2017/050951 WO2018027324A1 (en) 2016-08-12 2017-08-11 High thebaine poppy and methods of producing the same

Publications (1)

Publication Number Publication Date
CN110088284A true CN110088284A (zh) 2019-08-02

Family

ID=57243364

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780063464.9A Pending CN110088284A (zh) 2016-08-12 2017-08-11 高水平蒂巴因罂粟及其生产的方法

Country Status (11)

Country Link
US (2) US10808258B2 (https=)
EP (1) EP3334826B1 (https=)
JP (2) JP6906812B2 (https=)
CN (1) CN110088284A (https=)
AU (3) AU2017310339B2 (https=)
CA (1) CA2941315C (https=)
DK (1) DK3334826T5 (https=)
ES (1) ES2970324T3 (https=)
HU (1) HUE065349T2 (https=)
PT (1) PT3334826T (https=)
WO (1) WO2018027324A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3553177T3 (da) 2013-03-15 2021-02-15 Univ Leland Stanford Junior Benzylisoquinolinalkaloider (bia) som producerer mikrober, og fremgangsmåder til fremstilling og anvendelse deraf
CN112410379B (zh) 2013-11-04 2024-12-31 小利兰·斯坦福大学托管委员会 产生苄基异喹啉生物碱(bia)前体的微生物以及制备和使用所述前体的方法
EP3292203A4 (en) 2015-05-04 2019-10-09 The Board of Trustees of the Leland Stanford Junior University BENZYL ISOCHINOLIN ALKALOID (BIA) Precursor Producing Microbicides and Method for the Preparation and Use Thereof
JP7266966B2 (ja) 2015-05-08 2023-05-01 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー エピメラーゼ及びベンジルイソキノリンアルカロイドを産生する方法
WO2018075670A1 (en) 2016-10-18 2018-04-26 Antheia, Inc. Methods of producing nor-opioid and nal-opioid benzylisoquinoline alkaloids
AU2018203033B2 (en) * 2017-05-02 2021-07-01 Sun Pharmaceutical Industries Australia Pty Limited Modified plant
WO2019028390A1 (en) 2017-08-03 2019-02-07 Antheia, Inc. ALKALOID BENZYLISOQUINOLEIN GENETICALLY MODIFIED EPIMERASES AND METHODS FOR PRODUCING BENZYLISOQUINOLINE ALKALOIDS
EP3758728A4 (en) * 2018-03-02 2021-12-22 Tasmanian Alkaloids Pty. Ltd. PAPAVER SOMNIFERUM PLANT WITH AN MODIFIED ALKALOID PROFILE
CN120399912A (zh) 2019-03-08 2025-08-01 小利兰·斯坦福大学托管委员会 产生莨菪烷生物碱(ta)的非植物宿主细胞及其制备和使用方法
JP7555091B2 (ja) * 2020-02-29 2024-09-24 国立大学法人千葉大学 代謝物センサ及び酵素活性のスクリーニング方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104487576A (zh) * 2012-04-30 2015-04-01 Tpi企业有限公司 具有修饰的生物碱含量的大红罂粟

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6067749A (en) * 1996-07-11 2000-05-30 Tasmanian Alkaloids Pty. Ltd. Papaver somniferum strain with high concentration of thebaine and oripavine
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
CA2317508A1 (en) 1998-01-08 1999-07-15 Commonwealth Scientific And Industrial Research Organisation Methods for plant transformation and regeneration
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US6730838B1 (en) 1999-02-12 2004-05-04 Council Of Scientific And Industrial Research Opiumless and alkaloid-free non-narcotic opium poppy (Papaver somniferum) variety “Sujata”
AUPP946399A0 (en) 1999-03-26 1999-04-22 Johnson & Johnson Research Pty. Limited Codeinone reductase from alkaloid poppy
EP1270727A1 (en) 2001-06-11 2003-01-02 Institut für Pflanzenbiochemie Salutaridinol 7-O-acetyltransferase and derivatives thereof
CH695417A5 (de) * 2001-11-15 2006-05-15 Ddr Wassyl Nowicky Dipl Ing Verfahren zur Umsetzung von Alkaloiden.
US6790959B1 (en) 2003-06-05 2004-09-14 Halsey Drug Company, Inc. Process for manufacturing thebaine
US6864370B1 (en) 2003-06-05 2005-03-08 Zhaiwei Lin Process for manufacturing oxycodone
EP1512748A1 (en) 2003-09-03 2005-03-09 Johnson & Johnson Research Pty Limited O-methyltransferases of tetrahydrobenzylisoquinoline alkaloid biosynthesis in papaver somniferum
EP1632565A1 (en) 2004-08-12 2006-03-08 Johnson & Johnson Research Pty Limited Modulation of alkaloid biosynthesis in plants and plants having altered alkaloid biosynthesis
DE102005005449A1 (de) * 2005-02-04 2006-08-10 Grünenthal GmbH Verfahren zur Herstellung einer gegen Missbrauch gesicherten Darreichungsform
AU2006216715A1 (en) 2005-02-22 2006-08-31 Ceres Inc. Modulating plant alkaloids
WO2006115575A1 (en) 2005-04-20 2006-11-02 Ceres Inc. Regulatory regions from papaveraceae
US9131649B2 (en) * 2008-03-07 2015-09-15 Tasmanian Alkaloids Pty. Ltd Papaver somniferum strain with high concentration of thebaine
US8541647B2 (en) 2008-05-29 2013-09-24 Tasmania Alkaloids Pty, Ltd. Papaver somniferum with high concentration of codeine
US8586526B2 (en) 2010-05-17 2013-11-19 Sangamo Biosciences, Inc. DNA-binding proteins and uses thereof
WO2011058446A2 (en) * 2009-11-12 2011-05-19 Uti Limited Partnership Thebaine 6-o-demethylase and codeine o-demethylase from papaver somniferum

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104487576A (zh) * 2012-04-30 2015-04-01 Tpi企业有限公司 具有修饰的生物碱含量的大红罂粟

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FACCHINI ET AL.: "Opium poppy:a model system to investigate alkaloid biosynthesis in plants", 《CANADIAN JOURNAL OF BOTANY》 *
HAGEL ET AL.: "Dioxygenases catalyze the O-demethylation steps of morphine biosynthesis in opium poppy", 《NAT CHEM BIOL》 *
MILLGATE ET AL.: "Analgesia: morphine-pathway block in top1 poppies", 《NATURE》 *

Also Published As

Publication number Publication date
DK3334826T5 (da) 2024-10-07
ES2970324T3 (es) 2024-05-28
PT3334826T (pt) 2024-02-07
HUE065349T2 (hu) 2024-05-28
AU2017310339A1 (en) 2018-04-12
JP2021000097A (ja) 2021-01-07
US20190169625A1 (en) 2019-06-06
CA2941315A1 (en) 2016-11-08
EP3334826A1 (en) 2018-06-20
AU2019201771A1 (en) 2019-04-04
US10808258B2 (en) 2020-10-20
JP6906812B2 (ja) 2021-07-21
DK3334826T3 (da) 2024-01-15
JP2019528084A (ja) 2019-10-10
US20210246460A1 (en) 2021-08-12
WO2018027324A9 (en) 2019-07-18
CA2941315C (en) 2018-03-06
EP3334826A4 (en) 2018-08-08
EP3334826B1 (en) 2023-10-11
WO2018027324A1 (en) 2018-02-15
AU2017310339B2 (en) 2019-01-17
AU2021232757A1 (en) 2021-10-21

Similar Documents

Publication Publication Date Title
US20210246460A1 (en) High thebaine poppy and methods of producing the same
EP3036333B1 (en) Methods for producing genetic modifications in a plant genome without incorporating a selectable transgene marker, and compositions thereof
EP2728004B1 (en) Bacterial toxin vaccine
WO2014093768A1 (en) Precision gene targeting to a particular locus in maize
CN111630171A (zh) 植物抗倒伏性
CN116286742B (zh) CasD蛋白、CRISPR/CasD基因编辑系统及其在植物基因编辑中的应用
AU2010256356B2 (en) Isolation and targeted suppression of lignin biosynthetic genes from sugarcane
JP5594137B2 (ja) カプシノイドを生合成する遺伝子改変植物
MXPA04009927A (es) Acil-coa sintetasas vegetales.
CN111819285A (zh) 防碎基因和突变
WO2019075387A1 (en) METHODS AND COMPOSITIONS FOR MODULATING GOSSYPOL CONTENT IN COTTON PLANTS
CN106279386A (zh) 一种水稻穗顶部生长发育相关蛋白及其编码基因与应用
JP2003509027A (ja) クリーンな合成ベクター、プラスミド、トランスジェニック植物、及びこれらを含む植物の部分、並びにこれらを得る方法
US8669417B2 (en) Methods and compositions for increasing plant transformation efficiency
CN105566468A (zh) 植物育性相关蛋白及其应用
US20250320515A1 (en) Greenhouse-based production of fungal-derived medicinal compounds
WO2025042825A2 (en) Methods and means for influencing expression of heteroallelic genes or alleles in plants by modification of untranslated regions
CN105037522A (zh) 小麦卷叶相关蛋白TaMYB18及其相关生物材料与应用
WO2005054482A2 (en) Nucleic acids having utility in seeds
JP2002543782A (ja) 新規なトウモロコシｒａｄ５１様遺伝子およびその使用
CN104023521A (zh) 用于甘蔗中增加的表达的组合物和方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20190802