AU2017310339B2 - High thebaine poppy and methods of producing the same - Google Patents

High thebaine poppy and methods of producing the same Download PDF

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Publication number
AU2017310339B2
AU2017310339B2 AU2017310339A AU2017310339A AU2017310339B2 AU 2017310339 B2 AU2017310339 B2 AU 2017310339B2 AU 2017310339 A AU2017310339 A AU 2017310339A AU 2017310339 A AU2017310339 A AU 2017310339A AU 2017310339 B2 AU2017310339 B2 AU 2017310339B2
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plant
t6odm
codm
expression
thebaine
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AU2017310339A
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AU2017310339A1 (en
Inventor
Igor Kovalchuk
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Api Labs Inc
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Api Labs Inc
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Priority to AU2019201771A priority Critical patent/AU2019201771A1/en
Priority to AU2021232757A priority patent/AU2021232757A1/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47—Quinolines; Isoquinolines
    • A61K31/485—Morphinan derivatives, e.g. morphine, codeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/04—Centrally acting analgesics, e.g. opioids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C12Y114/11031—Thebaine 6-O-demethylase (1.14.11.31)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C12Y114/11032—Codeine 3-O-demethylase (1.14.11.32)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nutrition Science (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pain & Pain Management (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Emergency Medicine (AREA)
  • Epidemiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2017310339A 2016-08-12 2017-08-11 High thebaine poppy and methods of producing the same Active AU2017310339B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2019201771A AU2019201771A1 (en) 2016-08-12 2019-03-14 High thebaine poppy and methods of producing the same
AU2021232757A AU2021232757A1 (en) 2016-08-12 2021-09-16 High thebaine poppy and methods of producing the same

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662374682P 2016-08-12 2016-08-12
US62/374,682 2016-08-12
CA2,941,315 2016-09-07
CA2941315A CA2941315C (en) 2016-08-12 2016-09-07 High thebaine poppy and methods of producing the same
PCT/CA2017/050951 WO2018027324A1 (en) 2016-08-12 2017-08-11 High thebaine poppy and methods of producing the same

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2019201771A Division AU2019201771A1 (en) 2016-08-12 2019-03-14 High thebaine poppy and methods of producing the same

Publications (2)

Publication Number Publication Date
AU2017310339A1 AU2017310339A1 (en) 2018-04-12
AU2017310339B2 true AU2017310339B2 (en) 2019-01-17

Family

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Family Applications (3)

Application Number Title Priority Date Filing Date
AU2017310339A Active AU2017310339B2 (en) 2016-08-12 2017-08-11 High thebaine poppy and methods of producing the same
AU2019201771A Abandoned AU2019201771A1 (en) 2016-08-12 2019-03-14 High thebaine poppy and methods of producing the same
AU2021232757A Abandoned AU2021232757A1 (en) 2016-08-12 2021-09-16 High thebaine poppy and methods of producing the same

Family Applications After (2)

Application Number Title Priority Date Filing Date
AU2019201771A Abandoned AU2019201771A1 (en) 2016-08-12 2019-03-14 High thebaine poppy and methods of producing the same
AU2021232757A Abandoned AU2021232757A1 (en) 2016-08-12 2021-09-16 High thebaine poppy and methods of producing the same

Country Status (11)

Country Link
US (2) US10808258B2 (https=)
EP (1) EP3334826B1 (https=)
JP (2) JP6906812B2 (https=)
CN (1) CN110088284A (https=)
AU (3) AU2017310339B2 (https=)
CA (1) CA2941315C (https=)
DK (1) DK3334826T5 (https=)
ES (1) ES2970324T3 (https=)
HU (1) HUE065349T2 (https=)
PT (1) PT3334826T (https=)
WO (1) WO2018027324A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3553177T3 (da) 2013-03-15 2021-02-15 Univ Leland Stanford Junior Benzylisoquinolinalkaloider (bia) som producerer mikrober, og fremgangsmåder til fremstilling og anvendelse deraf
CN112410379B (zh) 2013-11-04 2024-12-31 小利兰·斯坦福大学托管委员会 产生苄基异喹啉生物碱(bia)前体的微生物以及制备和使用所述前体的方法
EP3292203A4 (en) 2015-05-04 2019-10-09 The Board of Trustees of the Leland Stanford Junior University BENZYL ISOCHINOLIN ALKALOID (BIA) Precursor Producing Microbicides and Method for the Preparation and Use Thereof
JP7266966B2 (ja) 2015-05-08 2023-05-01 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー エピメラーゼ及びベンジルイソキノリンアルカロイドを産生する方法
WO2018075670A1 (en) 2016-10-18 2018-04-26 Antheia, Inc. Methods of producing nor-opioid and nal-opioid benzylisoquinoline alkaloids
AU2018203033B2 (en) * 2017-05-02 2021-07-01 Sun Pharmaceutical Industries Australia Pty Limited Modified plant
WO2019028390A1 (en) 2017-08-03 2019-02-07 Antheia, Inc. ALKALOID BENZYLISOQUINOLEIN GENETICALLY MODIFIED EPIMERASES AND METHODS FOR PRODUCING BENZYLISOQUINOLINE ALKALOIDS
EP3758728A4 (en) * 2018-03-02 2021-12-22 Tasmanian Alkaloids Pty. Ltd. PAPAVER SOMNIFERUM PLANT WITH AN MODIFIED ALKALOID PROFILE
CN120399912A (zh) 2019-03-08 2025-08-01 小利兰·斯坦福大学托管委员会 产生莨菪烷生物碱(ta)的非植物宿主细胞及其制备和使用方法
JP7555091B2 (ja) * 2020-02-29 2024-09-24 国立大学法人千葉大学 代謝物センサ及び酵素活性のスクリーニング方法

Citations (3)

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Publication number Priority date Publication date Assignee Title
WO1998002033A1 (en) * 1996-07-11 1998-01-22 Tasmanian Alkaloids Pty. Ltd. Improved production of thebaine and oripavine
WO2009109012A1 (en) * 2008-03-07 2009-09-11 Tasmanian Alkaloids Pty. Ltd. Papaver somniferum with high concentration of thebaine
WO2011058446A2 (en) * 2009-11-12 2011-05-19 Uti Limited Partnership Thebaine 6-o-demethylase and codeine o-demethylase from papaver somniferum

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US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
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WO1998002033A1 (en) * 1996-07-11 1998-01-22 Tasmanian Alkaloids Pty. Ltd. Improved production of thebaine and oripavine
WO2009109012A1 (en) * 2008-03-07 2009-09-11 Tasmanian Alkaloids Pty. Ltd. Papaver somniferum with high concentration of thebaine
WO2011058446A2 (en) * 2009-11-12 2011-05-19 Uti Limited Partnership Thebaine 6-o-demethylase and codeine o-demethylase from papaver somniferum

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ALLEN R.S. et al., 'RNAi-mediated replacement of morphine with the nonnarcotic alkaloid reticuline in opium poppy', Nature Biotechnology. (2004), vol. 22, no. 12, pages 1559-1566. *
WIJEKOON, C.P. et al., "Systematic knockdown of morphine pathway enzymes in opium poppy using virus-induced gene silencing", The Plant Journal, (2012), vol. 69, ISSN 1365-313X, pages 1052-1063. *

Also Published As

Publication number Publication date
DK3334826T5 (da) 2024-10-07
ES2970324T3 (es) 2024-05-28
PT3334826T (pt) 2024-02-07
CN110088284A (zh) 2019-08-02
HUE065349T2 (hu) 2024-05-28
AU2017310339A1 (en) 2018-04-12
JP2021000097A (ja) 2021-01-07
US20190169625A1 (en) 2019-06-06
CA2941315A1 (en) 2016-11-08
EP3334826A1 (en) 2018-06-20
AU2019201771A1 (en) 2019-04-04
US10808258B2 (en) 2020-10-20
JP6906812B2 (ja) 2021-07-21
DK3334826T3 (da) 2024-01-15
JP2019528084A (ja) 2019-10-10
US20210246460A1 (en) 2021-08-12
WO2018027324A9 (en) 2019-07-18
CA2941315C (en) 2018-03-06
EP3334826A4 (en) 2018-08-08
EP3334826B1 (en) 2023-10-11
WO2018027324A1 (en) 2018-02-15
AU2021232757A1 (en) 2021-10-21

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