CN109844098A - 改进的分化方法 - Google Patents

改进的分化方法 Download PDF

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Publication number
CN109844098A
CN109844098A CN201780047555.3A CN201780047555A CN109844098A CN 109844098 A CN109844098 A CN 109844098A CN 201780047555 A CN201780047555 A CN 201780047555A CN 109844098 A CN109844098 A CN 109844098A
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cell
organoid
inhibitor
inhibitors
cells
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约翰尼斯·卡洛鲁斯·克莱威尔斯
乔珀·贝默
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Koninklijke Nederlandse Akademie van Wetenschappen
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CN201780047555.3A 2016-06-20 2017-06-20 改进的分化方法 Pending CN109844098A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1610748.4A GB201610748D0 (en) 2016-06-20 2016-06-20 Improved diffrentation method
GB1610748.4 2016-06-20
PCT/EP2017/065101 WO2017220586A1 (en) 2016-06-20 2017-06-20 Improved differentiation method

Publications (1)

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CN109844098A true CN109844098A (zh) 2019-06-04

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US (2) US20210047618A1 (enExample)
EP (1) EP3472302A1 (enExample)
JP (2) JP7525263B2 (enExample)
KR (2) KR102478617B1 (enExample)
CN (1) CN109844098A (enExample)
AU (1) AU2017281406B2 (enExample)
BR (1) BR112018076554A2 (enExample)
CA (1) CA3030098A1 (enExample)
GB (1) GB201610748D0 (enExample)
IL (1) IL263854A (enExample)
MX (2) MX2018016258A (enExample)
SG (1) SG11201811405QA (enExample)
WO (1) WO2017220586A1 (enExample)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113073080A (zh) * 2021-06-03 2021-07-06 北京科途医学科技有限公司 一种多形性横纹肌肉瘤类器官及其培养方法、培养基和用途
CN113151152A (zh) * 2021-05-11 2021-07-23 中国人民解放军军事科学院军事医学研究院 一种小鼠肺类器官的培养方法及其专用培养液
CN113403277A (zh) * 2021-06-28 2021-09-17 山东省第二人民医院(山东省耳鼻喉医院、山东省耳鼻喉研究所) 一种分离内耳fzd10阳性胶质细胞的方法
WO2021254296A1 (zh) * 2020-06-14 2021-12-23 浙江大学 一种生物活性物质组合物、包含所述组合物的无血清培养基及其用途
CN119931962A (zh) * 2025-04-07 2025-05-06 北京华诺泰生物医药科技有限公司 一种人偏肺病毒的培养方法

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011140441A2 (en) 2010-05-06 2011-11-10 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
BR112014014756B1 (pt) 2011-12-19 2022-12-06 Koninklijke Nederlandse Akademie Van Wetenschappen Método in vitro, e, uso in vitro de um ou mais organoides
AU2015261380B2 (en) 2014-05-16 2021-04-15 Koninklijke Nederlandse Akademie Van Wetenschappen Improved culture method for organoids
JP6687544B2 (ja) 2014-05-28 2020-04-22 チルドレンズ ホスピタル メディカル センター 前駆細胞を指向性分化によって胃組織に変換するための方法及びシステム
US11584916B2 (en) 2014-10-17 2023-02-21 Children's Hospital Medical Center Method of making in vivo human small intestine organoids from pluripotent stem cells
GB201421094D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201421092D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201603569D0 (en) 2016-03-01 2016-04-13 Koninklijke Nederlandse Akademie Van Wetenschappen Improved differentiation method
CN109415685B (zh) 2016-05-05 2023-07-04 儿童医院医疗中心 用于体外制造胃底组织的方法和与其相关的组合物
SG11201903697WA (en) 2016-11-04 2019-05-30 Childrens Hospital Med Ct Liver organoid compositions and methods of making and using same
NZ753873A (en) 2016-12-05 2023-01-27 Children’S Hospital Medical Center Colonic organoids and methods of making and using same
WO2018191673A1 (en) 2017-04-14 2018-10-18 Children's Hospital Medical Center Multi donor stem cell compositions and methods of making same
JP2020536529A (ja) 2017-10-10 2020-12-17 チルドレンズ ホスピタル メディカル センター 食道組織および/または臓器組成物およびそれを作製する方法
EP3727394A4 (en) 2017-12-21 2021-09-08 Children's Hospital Medical Center DIGITALIZED HUMAN ORGANOIDS AND METHOD OF USING THEREOF
US11590152B2 (en) * 2018-01-26 2023-02-28 Massachusetts Eye And Ear Infirmary Treatment of hearing loss
WO2019185017A1 (zh) * 2018-03-30 2019-10-03 中国科学院上海生命科学研究院 用于肝细胞培养及肝脏类器官制备的培养基
AU2019266240A1 (en) * 2018-05-07 2021-01-07 President And Fellows Of Harvard College Cell populations and gene expression associated with in vitro beta cell differentiation
CA3106634A1 (en) 2018-07-26 2020-01-30 Children's Hospital Medical Center Hepato-biliary-pancreatic tissues and methods of making same
JP7377486B2 (ja) * 2018-09-10 2023-11-10 国立大学法人東京工業大学 多能性幹細胞から腸細胞の作製方法
AU2019339410A1 (en) 2018-09-12 2021-04-15 Children's Hospital Medical Center Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
GB201819224D0 (en) 2018-11-26 2019-01-09 Koninklijke Nederlandse Akademie Van Wetenschappen Hepatocyte expansion methods
KR20200065892A (ko) 2018-11-30 2020-06-09 오가노이드사이언스 주식회사 오가노이드의 생체 이식용 조성물
KR102133693B1 (ko) * 2018-12-28 2020-07-13 서울대학교병원 타액선 유래 줄기세포를 타액선 조직으로 분화시키는 방법 및 구강 건조증 치료 또는 예방용 조성물
CN111500525B (zh) * 2019-01-30 2023-05-02 中国科学院广州生物医药与健康研究院 一种组合物及其应用
US12221630B2 (en) 2019-02-19 2025-02-11 Miltenyi Biotec B.V. & Co. KG Cell culture medium and method for generation of epithelial organoids from epithelial stem cells
WO2020172792A1 (en) * 2019-02-26 2020-09-03 Peking University Compositions and methods for long term culture of hepatocytes
GB201906978D0 (en) 2019-05-17 2019-07-03 Koninklijke Nederlandse Akademie Van Wetenschappen Improved culture method using integrin agonist
JP7610533B2 (ja) 2019-05-31 2025-01-08 チルドレンズ ホスピタル メディカル センター 造血幹細胞の生成および拡大方法
WO2021055565A1 (en) * 2019-09-17 2021-03-25 The Trustees Of Indiana University Prolonged function of liver organoids as an in vitro model for the study of liver disease
EP4048809A4 (en) 2019-10-22 2024-02-14 Tempus Labs, Inc. Systems and methods for predicting therapeutic sensitivity
US11629385B2 (en) 2019-11-22 2023-04-18 Tempus Labs, Inc. Tumor organoid culture compositions, systems, and methods
KR102232244B1 (ko) * 2019-11-29 2021-03-24 고려대학교 산학협력단 Mhy1485 또는 이의 유도체를 포함하는 체세포로부터 유도만능줄기세포로의 역분화 효율 증진용 조성물 및 이를 이용한 역분화 효율 증진 방법
JP7744340B2 (ja) 2019-12-05 2025-09-25 テンパス・エーアイ・インコーポレイテッド ハイスループット薬物スクリーニングのためのシステムおよび方法
US20230029554A1 (en) * 2019-12-12 2023-02-02 The Walter And Eliza Hall Institute Of Medecal Research Organoid cultures
GB202004706D0 (en) 2020-03-31 2020-05-13 Koninklijke Nederlandse Akademie Van Wetenschappen Methods and tools for studying enteroendocrine cells
US11561178B2 (en) 2020-04-20 2023-01-24 Tempus Labs, Inc. Artificial fluorescent image systems and methods
US20220135950A1 (en) * 2020-11-03 2022-05-05 Korea Research Institute Of Bioscience And Biotechnology Human intestinal epithelium model and method for preparing same
US20240070940A1 (en) * 2021-01-12 2024-02-29 Astrazeneca Ab Graph construction and visualization of multiplex immunofluorescence images
KR20230161494A (ko) 2021-03-30 2023-11-27 트레일헤드 바이오시스템즈 인크. 희소돌기아교세포 전구 세포를 생성하기 위한 방법 및 조성물
WO2022231904A1 (en) * 2021-04-27 2022-11-03 Avita Medical, Inc. Regenerative bioactive suspension derived from freshly disaggregated tissue and methods of use in clinical therapies
WO2022241303A1 (en) * 2021-05-14 2022-11-17 University Of South Florida Composition and method for treating covid-19
GB202109913D0 (en) 2021-07-09 2021-08-25 Stichting Hubrecht Organoid Tech Organoid-derived monolayers and uses thereof
WO2023074648A1 (ja) * 2021-10-25 2023-05-04 富士フイルム株式会社 腸内分泌細胞を製造する方法、多能性幹細胞由来の腸内分泌細胞、培地又は培地キット、およびそれらの利用
EP4423252A1 (en) * 2021-10-29 2024-09-04 Trailhead Biosystems Inc. Methods and compositions for generating human forebrain neural progenitor cells and for maturation thereof to parvalbumin+ interneurons
KR20240088578A (ko) * 2022-11-24 2024-06-20 한국생명공학연구원 박막 코팅된 표면 위에서 장 줄기세포를 무이종 배양하는 법
CN116478912A (zh) 2023-06-07 2023-07-25 合肥工业大学 一种诱导小鼠肠道类器官定向分化为肠内分泌细胞的培养基及其应用
EP4495227A1 (en) 2023-07-18 2025-01-22 HUB Organoids IP B.V. Co-cultures of organoids and stromal cells
WO2025111693A1 (en) * 2023-11-30 2025-06-05 University Health Network Generation of enterochromaffin and pancreatic cells
KR20250087176A (ko) * 2023-12-07 2025-06-16 한국화학연구원 역분화 줄기세포 유래 염증성 장 질환 모델, 이의 제조 방법 및 이를 이용한 약물 효능 평가 방법

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102439135A (zh) * 2009-02-03 2012-05-02 荷兰皇家科学院 用于上皮干细胞和包含所述干细胞的类器官的培养基
WO2014159356A1 (en) * 2013-03-14 2014-10-02 The Brigham And Women's Hospital, Inc. Compositions and methods for epithelial stem cell expansion and culture
CN108779437A (zh) * 2016-01-08 2018-11-09 麻省理工学院 分化的肠内分泌细胞和胰岛素产生细胞的制备

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140328808A1 (en) * 2011-10-27 2014-11-06 National University Corporation Tokyo Medical And Dental University Technique for isolating/culturing colorectal epithelial stem cell, and technique for transplanting colorectal epithelium employing said technique
SG10201803131TA (en) * 2013-02-18 2018-06-28 Univ Health Network Methods for generating hepatocytes and cholangiocytes from pluripotent stem cells
US10487314B2 (en) 2014-06-26 2019-11-26 The Trustees Of Columbia University In The City Of New York Inhibition of serotonin expression in gut enteroendocrine cells results in conversion to insulin-positive cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102439135A (zh) * 2009-02-03 2012-05-02 荷兰皇家科学院 用于上皮干细胞和包含所述干细胞的类器官的培养基
WO2014159356A1 (en) * 2013-03-14 2014-10-02 The Brigham And Women's Hospital, Inc. Compositions and methods for epithelial stem cell expansion and culture
CN108779437A (zh) * 2016-01-08 2018-11-09 麻省理工学院 分化的肠内分泌细胞和胰岛素产生细胞的制备

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JULIAN HEUBERGER等: "Shp2/MAPK signaling controls goblet/paneth cell fatedecisions in the intestine", 《PNAS》 *
XIAOLEI YIN等: "Niche-independent high-purity cultures of Lgr5+ intestinal stem cells and their progeny", 《NATURE METHODS》 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021254296A1 (zh) * 2020-06-14 2021-12-23 浙江大学 一种生物活性物质组合物、包含所述组合物的无血清培养基及其用途
CN113151152A (zh) * 2021-05-11 2021-07-23 中国人民解放军军事科学院军事医学研究院 一种小鼠肺类器官的培养方法及其专用培养液
CN113073080A (zh) * 2021-06-03 2021-07-06 北京科途医学科技有限公司 一种多形性横纹肌肉瘤类器官及其培养方法、培养基和用途
CN113073080B (zh) * 2021-06-03 2021-08-13 北京科途医学科技有限公司 一种多形性横纹肌肉瘤类器官及其培养方法、培养基和用途
CN113403277A (zh) * 2021-06-28 2021-09-17 山东省第二人民医院(山东省耳鼻喉医院、山东省耳鼻喉研究所) 一种分离内耳fzd10阳性胶质细胞的方法
CN113403277B (zh) * 2021-06-28 2023-05-16 山东省第二人民医院(山东省耳鼻喉医院、山东省耳鼻喉研究所) 一种分离内耳fzd10阳性胶质细胞的方法
CN119931962A (zh) * 2025-04-07 2025-05-06 北京华诺泰生物医药科技有限公司 一种人偏肺病毒的培养方法
CN119931962B (zh) * 2025-04-07 2025-08-05 北京华诺泰生物医药科技有限公司 一种人偏肺病毒的培养方法

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