CN109804073B - 用于高效生成瑞鲍迪苷的udp依赖性糖基转移酶 - Google Patents

用于高效生成瑞鲍迪苷的udp依赖性糖基转移酶 Download PDF

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CN109804073B
CN109804073B CN201780049312.3A CN201780049312A CN109804073B CN 109804073 B CN109804073 B CN 109804073B CN 201780049312 A CN201780049312 A CN 201780049312A CN 109804073 B CN109804073 B CN 109804073B
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modified host
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CN109804073A (zh
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赵立山
李文宗
G.维施曼
A.坎科耶
C.加西亚德根扎罗
T.马哈德奎-美亚道斯
S.杰克逊
M.利威尔
D.普拉特
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Amyris Inc
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    • C—CHEMISTRY; METALLURGY
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    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
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    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
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    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
    • A—HUMAN NECESSITIES
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    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
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    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/24—Non-sugar sweeteners
    • A23V2250/262—Stevioside

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CN201780049312.3A 2016-08-12 2017-08-11 用于高效生成瑞鲍迪苷的udp依赖性糖基转移酶 Active CN109804073B (zh)

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US201662374408P 2016-08-12 2016-08-12
US62/374408 2016-08-12
PCT/US2017/046637 WO2018031955A2 (en) 2016-08-12 2017-08-11 Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides

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CN109804073B true CN109804073B (zh) 2023-05-16

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US (3) US11091743B2 (cg-RX-API-DMAC7.html)
EP (1) EP3497222B1 (cg-RX-API-DMAC7.html)
JP (2) JP7404068B2 (cg-RX-API-DMAC7.html)
KR (1) KR102331018B1 (cg-RX-API-DMAC7.html)
CN (1) CN109804073B (cg-RX-API-DMAC7.html)
AU (1) AU2017308143B2 (cg-RX-API-DMAC7.html)
BR (1) BR112018074999A2 (cg-RX-API-DMAC7.html)
CA (1) CA3031162A1 (cg-RX-API-DMAC7.html)
ES (1) ES2906310T3 (cg-RX-API-DMAC7.html)
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PT (1) PT3497222T (cg-RX-API-DMAC7.html)
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Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112017005656A2 (pt) 2014-09-19 2018-07-31 Purecircle Sdn Bhd glicosídeos de esteviol de alta pureza
ES2906310T3 (es) * 2016-08-12 2022-04-18 Amyris Inc Glicosiltransferasa dependiente de UDP para la producción de alta eficiencia de rebaudiósidos
CN110914445B (zh) 2017-02-03 2024-08-27 泰莱解决方案美国有限责任公司 工程化糖基转移酶和甜菊醇糖苷葡糖基化方法
SG10202112698PA (en) * 2017-05-15 2021-12-30 Purecircle Usa Inc High-purity steviol glycosides
CN111868252B (zh) * 2018-03-12 2025-06-03 科纳根公司 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产
US11608364B2 (en) 2018-04-30 2023-03-21 Dsm Ip Assets B.V. Steviol glycoside transport
JP7584401B2 (ja) 2018-07-30 2024-11-15 テイト アンド ライル ソリューションズ ユー・エス・エー エル・エル・シー 操作されたグリコシルトランスフェラーゼおよびステビオールグリコシドのグルコシル化方法
EP3867370A1 (en) 2018-10-15 2021-08-25 Amyris, Inc. Stevia rebaudiana kaurenoic acid hydroxylase variants for high efficiency production of rebaudiosides
CN113302298B (zh) 2018-11-09 2025-02-25 银杏生物制品公司 罗汉果甙的生物合成
SG11202107656TA (en) 2019-01-24 2021-08-30 Amyris Inc Abc transporters for the high efficiency production of rebaudiosides
SG11202111698SA (en) 2019-05-03 2021-11-29 Amyris Inc Kaurenoic acid 13-hydroxylase (kah) variants and uses thereof
MX2022011329A (es) * 2020-03-13 2022-10-07 Amyris Inc Composiciones edulcorantes de rebaudiosido m.
CN111518782B (zh) * 2020-03-24 2022-09-02 广东广业清怡食品科技股份有限公司 一种糖基转移酶ugtzj1突变体及其应用
CN111424065B (zh) * 2020-03-24 2022-08-16 金禾益康(北京)生物科技有限公司 使用糖基转移酶对甜菊糖苷类化合物进行糖基化方法
EP4269573A4 (en) * 2020-12-23 2025-07-23 Samyang Corp GLYCOSYLTRANSFERASE AND METHOD FOR PREPARING STEVIOL GLUCOSIDE USING SAME
WO2022216922A1 (en) * 2021-04-08 2022-10-13 Conagen Inc. Biosynthetic production of steviol glycosides
CN115478060B (zh) * 2021-06-16 2023-11-28 弈柯莱生物科技(集团)股份有限公司 一种糖基转移酶及其应用
KR102628374B1 (ko) * 2021-10-19 2024-01-24 씨제이제일제당 주식회사 신규한 당전이효소 및 이의 용도
JP2024540897A (ja) * 2021-10-19 2024-11-06 シージェイ チェイルジェダン コーポレーション レバウジオシドｄ及びレバウジオシドｍを製造する方法
CN114045273B (zh) * 2021-11-15 2022-07-22 四川大学 糖基转移酶OsUGT91C1突变体及其应用
JP2025500510A (ja) * 2021-12-24 2025-01-09 サムヤン コーポレイション 糖転移酵素変異体、およびこれを利用したステビオール配糖体の製造方法
US20250320538A1 (en) * 2022-05-19 2025-10-16 Amyris, Inc. Compositions and methods for improved production of steviol glycosides
WO2024042405A1 (en) 2022-08-26 2024-02-29 Amyris Bio Products Portugal, Unipessoal, Ltda. Compositions and methods for the synthesis of bio-based polymers
WO2024042486A1 (en) 2022-08-26 2024-02-29 Amyris Bio Products Portugal, Unipessoal, Ltda. Compositions and methods for the production of polyurethanes
WO2024116153A1 (en) 2022-12-02 2024-06-06 Amyris Bio Products Portugal, Unipessoal, Ltda. Compositions and methods for using previously cultured cells
WO2025159542A1 (ko) * 2024-01-24 2025-07-31 씨제이제일제당 (주) 신규한 변이형 당전이 효소 폴리펩티드 및 이를 이용한 스테비올배당체 생산 방법

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013022989A2 (en) * 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
WO2014122227A2 (en) * 2013-02-06 2014-08-14 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
WO2014193888A1 (en) * 2013-05-28 2014-12-04 Purecircle Sdn Bhd High-purity steviol glycosides
WO2016038095A2 (en) * 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts

Family Cites Families (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7838044B2 (en) 2004-12-21 2010-11-23 Purecircle Sdn Bhd Extraction, separation and modification of sweet glycosides from the Stevia rebaudiana plant
ES2592377T3 (es) 2008-10-03 2016-11-29 Morita Kagaku Kogyo Co., Ltd. Nuevos glucósidos de esteviol
JP4760951B2 (ja) 2009-05-08 2011-08-31 トヨタ自動車株式会社 ブタノール生産能を有する組換え微生物及びブタノールの製造方法
PL2498625T3 (pl) 2009-11-12 2019-07-31 Purecircle Usa Inc. Granulacja słodzika stewia
US10888099B2 (en) 2009-11-12 2021-01-12 Purecircle Sdn Bhd. Granulation of a Stevia sweetener
EP4108668A1 (en) 2010-03-12 2022-12-28 PureCircle USA Inc. High-purity steviol glycosides
JP5056897B2 (ja) 2010-05-14 2012-10-24 トヨタ自動車株式会社 ２−ブタノールの製造方法及び２−ブタノール生産能を有する組換え微生物
NZ604915A (en) 2010-06-02 2014-10-31 Evolva Nutrition Inc Recombinant production of steviol glycosides
WO2012075030A1 (en) * 2010-11-30 2012-06-07 Massachusetts Institute Of Technology Microbial production of natural sweeteners, diterpenoid steviol glycosides
WO2012135591A2 (en) 2011-03-30 2012-10-04 Amyris, Inc. Microbial isoprenoid production using a heterologous dxp pathway
DK2806754T3 (en) 2012-01-23 2019-02-18 Dsm Ip Assets Bv Diterpene PREPARATION
US10000783B2 (en) 2012-03-16 2018-06-19 Suntory Holdings Limited Steviol glucosyltransferases and genes encoding the same
US10113154B2 (en) 2012-03-27 2018-10-30 Suntory Holdings Limited Method for producing steviol glycoside
CN110358795B (zh) 2012-05-22 2024-12-03 谱赛科有限责任公司 高纯度的甜菊醇糖苷
TW201402599A (zh) 2012-05-30 2014-01-16 Suntory Holdings Ltd 甜菊糖苷糖苷轉化酵素及編碼該酵素之基因
CN105189771B (zh) * 2013-02-11 2019-11-05 埃沃尔瓦公司 在重组宿主中有效产生甜菊醇糖苷
KR101404728B1 (ko) 2013-02-28 2014-06-09 씨제이제일제당 (주) 스테비오사이드로부터 리바우디오사이드 a를 제조하는 방법
PE20151544A1 (es) 2013-03-15 2015-11-04 Cargill Inc Plantas de stevia con un contenido de rebaudiosido d incrementado
WO2014191581A2 (en) 2013-05-31 2014-12-04 Dsm Ip Assets B.V. Microorganisms for diterpene production
MX2016000452A (es) 2013-07-15 2016-05-12 Dsm Ip Assets Bv Produccion de diterpeno.
MX369591B (es) 2013-07-31 2019-11-13 Dsm Ip Assets Bv Recuperacion de glicosidos de esteviol.
EP3030662B1 (en) 2013-08-07 2020-01-15 Amyris, Inc. Methods for stabilizing production of acetyl-coenzyme a derived compounds
BR112017002783A2 (pt) 2014-08-11 2017-12-19 Evolva Sa produção de glicosídeos de esteviol em hospedeiros recombinantes
PL3183349T3 (pl) 2014-08-19 2021-05-04 Purecircle Sdn Bhd Sposób przygotowania Rebaudiozydu I
DK3201351T3 (da) 2014-10-03 2021-02-22 Conagen Inc Kaloriefrie sødestoffer og fremgangsmåder til syntetisering
US10463062B2 (en) 2014-11-05 2019-11-05 Manus Bio Inc. Microbial production of steviol glycosides
JP2018503388A (ja) 2015-01-30 2018-02-08 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． 組み換え宿主におけるステビオールグリコシドの生産
CN104726523B (zh) 2015-03-28 2018-08-10 南京工业大学 一种酶法制备莱鲍迪苷m的方法
EP3302097B1 (en) 2015-05-29 2022-03-23 Cargill, Incorporated Heat treatment to produce glycosides
US10815513B2 (en) 2015-05-29 2020-10-27 Cargill, Incorporated Fermentation methods for producing steviol glycosides using high pH and compositions obtained therefrom
WO2017009293A1 (en) 2015-07-10 2017-01-19 Dsm Ip Assets B.V. Steviol glycoside composition
EP3320104B1 (en) 2015-07-10 2021-11-10 DSM IP Assets B.V. Method for preparing a steviol glycoside composition
WO2017025649A1 (en) 2015-08-13 2017-02-16 Dsm Ip Assets B.V. Steviol glycoside transport
WO2017093895A1 (en) 2015-11-30 2017-06-08 Purecircle Sdn Bhd Process for producing high purity steviol glycosides
JP2019503670A (ja) 2015-12-10 2019-02-14 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． 組換え宿主内でのステビオールグリコシドの産生
ES2877475T3 (es) * 2016-03-01 2021-11-16 Univation Tech Llc Método para mejorar la actividad del catalizador ziegler-natta
SG11201808856TA (en) 2016-04-13 2018-11-29 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2017198682A1 (en) 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
AU2017267214A1 (en) 2016-05-16 2018-11-15 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3497109A1 (en) 2016-08-09 2019-06-19 DSM IP Assets B.V. Crystallization of steviol glycosides
ES2906310T3 (es) * 2016-08-12 2022-04-18 Amyris Inc Glicosiltransferasa dependiente de UDP para la producción de alta eficiencia de rebaudiósidos
EP3535406A1 (en) 2016-11-07 2019-09-11 Evolva SA Production of steviol glycosides in recombinant hosts
SG10202112698PA (en) 2017-05-15 2021-12-30 Purecircle Usa Inc High-purity steviol glycosides
MA49836A (fr) * 2017-08-11 2020-06-17 Amyris Inc Kaurène oxydase de pisum sativum pour la production à haut rendement de rebaudiosides
US20200347425A1 (en) 2017-11-20 2020-11-05 Manus Bio, Inc. Microbial host cells for production of steviol glycosides
US20190270971A1 (en) 2018-03-01 2019-09-05 Jason Donald Increasing productivity of microbial host cells that functionally express p450 enzymes
GB201805576D0 (en) 2018-04-04 2018-05-16 Optibiotix Ltd Sweeteners and methods of production thereof
US11608364B2 (en) 2018-04-30 2023-03-21 Dsm Ip Assets B.V. Steviol glycoside transport
JP7584401B2 (ja) 2018-07-30 2024-11-15 テイト アンド ライル ソリューションズ ユー・エス・エー エル・エル・シー 操作されたグリコシルトランスフェラーゼおよびステビオールグリコシドのグルコシル化方法
KR102709594B1 (ko) 2019-06-25 2024-09-26 마누스 바이오, 인크. 우리딘 다이포스페이트-의존성 글라이코실트랜스퍼라제 효소

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013022989A2 (en) * 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
WO2014122227A2 (en) * 2013-02-06 2014-08-14 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
WO2014193888A1 (en) * 2013-05-28 2014-12-04 Purecircle Sdn Bhd High-purity steviol glycosides
WO2016038095A2 (en) * 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Development of Next Generation Stevia Sweetener: Rebaudioside M;INDRA PRAKASH等;《FOODS》;20140227;第3卷(第1期);第162-175页 *
NCBI Reference Sequence: XP_004982059.1;无;《GenBank》;20151130;Sequence *
无.NCBI Reference Sequence: XP_004982059.1.《GenBank》.2015,Sequence. *

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