CA3031162A1 - Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides - Google Patents

Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides Download PDF

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Publication number
CA3031162A1
CA3031162A1 CA3031162A CA3031162A CA3031162A1 CA 3031162 A1 CA3031162 A1 CA 3031162A1 CA 3031162 A CA3031162 A CA 3031162A CA 3031162 A CA3031162 A CA 3031162A CA 3031162 A1 CA3031162 A1 CA 3031162A1
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CA
Canada
Prior art keywords
seq
amino acid
udp
glycosyltransferase
genetically modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3031162A
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English (en)
French (fr)
Inventor
Lishan Zhao
Wenzong LI
Gale WICHMANN
Aditi KHANKHOJE
Chantal GARCIA DE GONZALO
Tina MAHATDEJKUL-MEADOWS
Shaina Jackson
Michael Leavell
Darren Platt
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Amyris Inc
Original Assignee
Amyris Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Inc filed Critical Amyris Inc
Publication of CA3031162A1 publication Critical patent/CA3031162A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/24—Non-sugar sweeteners
    • A23V2250/262—Stevioside

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Polymers & Plastics (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Seasonings (AREA)
CA3031162A 2016-08-12 2017-08-11 Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides Pending CA3031162A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662374408P 2016-08-12 2016-08-12
US62/374,408 2016-08-12
PCT/US2017/046637 WO2018031955A2 (en) 2016-08-12 2017-08-11 Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides

Publications (1)

Publication Number Publication Date
CA3031162A1 true CA3031162A1 (en) 2018-02-15

Family

ID=59700219

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3031162A Pending CA3031162A1 (en) 2016-08-12 2017-08-11 Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides

Country Status (14)

Country Link
US (3) US11091743B2 (cg-RX-API-DMAC7.html)
EP (1) EP3497222B1 (cg-RX-API-DMAC7.html)
JP (2) JP7404068B2 (cg-RX-API-DMAC7.html)
KR (1) KR102331018B1 (cg-RX-API-DMAC7.html)
CN (1) CN109804073B (cg-RX-API-DMAC7.html)
AU (1) AU2017308143B2 (cg-RX-API-DMAC7.html)
BR (1) BR112018074999A2 (cg-RX-API-DMAC7.html)
CA (1) CA3031162A1 (cg-RX-API-DMAC7.html)
ES (1) ES2906310T3 (cg-RX-API-DMAC7.html)
MX (1) MX2019001631A (cg-RX-API-DMAC7.html)
MY (1) MY194280A (cg-RX-API-DMAC7.html)
PT (1) PT3497222T (cg-RX-API-DMAC7.html)
SG (1) SG11201900930UA (cg-RX-API-DMAC7.html)
WO (1) WO2018031955A2 (cg-RX-API-DMAC7.html)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112017005656A2 (pt) 2014-09-19 2018-07-31 Purecircle Sdn Bhd glicosídeos de esteviol de alta pureza
ES2906310T3 (es) * 2016-08-12 2022-04-18 Amyris Inc Glicosiltransferasa dependiente de UDP para la producción de alta eficiencia de rebaudiósidos
CN110914445B (zh) 2017-02-03 2024-08-27 泰莱解决方案美国有限责任公司 工程化糖基转移酶和甜菊醇糖苷葡糖基化方法
SG10202112698PA (en) * 2017-05-15 2021-12-30 Purecircle Usa Inc High-purity steviol glycosides
CN111868252B (zh) * 2018-03-12 2025-06-03 科纳根公司 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产
US11608364B2 (en) 2018-04-30 2023-03-21 Dsm Ip Assets B.V. Steviol glycoside transport
JP7584401B2 (ja) 2018-07-30 2024-11-15 テイト アンド ライル ソリューションズ ユー・エス・エー エル・エル・シー 操作されたグリコシルトランスフェラーゼおよびステビオールグリコシドのグルコシル化方法
EP3867370A1 (en) 2018-10-15 2021-08-25 Amyris, Inc. Stevia rebaudiana kaurenoic acid hydroxylase variants for high efficiency production of rebaudiosides
CN113302298B (zh) 2018-11-09 2025-02-25 银杏生物制品公司 罗汉果甙的生物合成
SG11202107656TA (en) 2019-01-24 2021-08-30 Amyris Inc Abc transporters for the high efficiency production of rebaudiosides
SG11202111698SA (en) 2019-05-03 2021-11-29 Amyris Inc Kaurenoic acid 13-hydroxylase (kah) variants and uses thereof
MX2022011329A (es) * 2020-03-13 2022-10-07 Amyris Inc Composiciones edulcorantes de rebaudiosido m.
CN111518782B (zh) * 2020-03-24 2022-09-02 广东广业清怡食品科技股份有限公司 一种糖基转移酶ugtzj1突变体及其应用
CN111424065B (zh) * 2020-03-24 2022-08-16 金禾益康(北京)生物科技有限公司 使用糖基转移酶对甜菊糖苷类化合物进行糖基化方法
EP4269573A4 (en) * 2020-12-23 2025-07-23 Samyang Corp GLYCOSYLTRANSFERASE AND METHOD FOR PREPARING STEVIOL GLUCOSIDE USING SAME
WO2022216922A1 (en) * 2021-04-08 2022-10-13 Conagen Inc. Biosynthetic production of steviol glycosides
CN115478060B (zh) * 2021-06-16 2023-11-28 弈柯莱生物科技(集团)股份有限公司 一种糖基转移酶及其应用
KR102628374B1 (ko) * 2021-10-19 2024-01-24 씨제이제일제당 주식회사 신규한 당전이효소 및 이의 용도
JP2024540897A (ja) * 2021-10-19 2024-11-06 シージェイ チェイルジェダン コーポレーション レバウジオシドｄ及びレバウジオシドｍを製造する方法
CN114045273B (zh) * 2021-11-15 2022-07-22 四川大学 糖基转移酶OsUGT91C1突变体及其应用
JP2025500510A (ja) * 2021-12-24 2025-01-09 サムヤン コーポレイション 糖転移酵素変異体、およびこれを利用したステビオール配糖体の製造方法
US20250320538A1 (en) * 2022-05-19 2025-10-16 Amyris, Inc. Compositions and methods for improved production of steviol glycosides
WO2024042405A1 (en) 2022-08-26 2024-02-29 Amyris Bio Products Portugal, Unipessoal, Ltda. Compositions and methods for the synthesis of bio-based polymers
WO2024042486A1 (en) 2022-08-26 2024-02-29 Amyris Bio Products Portugal, Unipessoal, Ltda. Compositions and methods for the production of polyurethanes
WO2024116153A1 (en) 2022-12-02 2024-06-06 Amyris Bio Products Portugal, Unipessoal, Ltda. Compositions and methods for using previously cultured cells
WO2025159542A1 (ko) * 2024-01-24 2025-07-31 씨제이제일제당 (주) 신규한 변이형 당전이 효소 폴리펩티드 및 이를 이용한 스테비올배당체 생산 방법

Family Cites Families (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7838044B2 (en) 2004-12-21 2010-11-23 Purecircle Sdn Bhd Extraction, separation and modification of sweet glycosides from the Stevia rebaudiana plant
ES2592377T3 (es) 2008-10-03 2016-11-29 Morita Kagaku Kogyo Co., Ltd. Nuevos glucósidos de esteviol
JP4760951B2 (ja) 2009-05-08 2011-08-31 トヨタ自動車株式会社 ブタノール生産能を有する組換え微生物及びブタノールの製造方法
PL2498625T3 (pl) 2009-11-12 2019-07-31 Purecircle Usa Inc. Granulacja słodzika stewia
US10888099B2 (en) 2009-11-12 2021-01-12 Purecircle Sdn Bhd. Granulation of a Stevia sweetener
EP4108668A1 (en) 2010-03-12 2022-12-28 PureCircle USA Inc. High-purity steviol glycosides
JP5056897B2 (ja) 2010-05-14 2012-10-24 トヨタ自動車株式会社 ２−ブタノールの製造方法及び２−ブタノール生産能を有する組換え微生物
NZ604915A (en) 2010-06-02 2014-10-31 Evolva Nutrition Inc Recombinant production of steviol glycosides
WO2012075030A1 (en) * 2010-11-30 2012-06-07 Massachusetts Institute Of Technology Microbial production of natural sweeteners, diterpenoid steviol glycosides
WO2012135591A2 (en) 2011-03-30 2012-10-04 Amyris, Inc. Microbial isoprenoid production using a heterologous dxp pathway
SG10201606567TA (en) 2011-08-08 2016-10-28 Evolva Sa Recombinant production of steviol glycosides
DK2806754T3 (en) 2012-01-23 2019-02-18 Dsm Ip Assets Bv Diterpene PREPARATION
US10000783B2 (en) 2012-03-16 2018-06-19 Suntory Holdings Limited Steviol glucosyltransferases and genes encoding the same
US10113154B2 (en) 2012-03-27 2018-10-30 Suntory Holdings Limited Method for producing steviol glycoside
CN110358795B (zh) 2012-05-22 2024-12-03 谱赛科有限责任公司 高纯度的甜菊醇糖苷
US9752174B2 (en) * 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
TW201402599A (zh) 2012-05-30 2014-01-16 Suntory Holdings Ltd 甜菊糖苷糖苷轉化酵素及編碼該酵素之基因
CN105051195B (zh) 2013-02-06 2019-09-06 埃沃尔瓦公司 用于提高莱鲍迪苷d和莱鲍迪苷m之产生的方法
CN105189771B (zh) * 2013-02-11 2019-11-05 埃沃尔瓦公司 在重组宿主中有效产生甜菊醇糖苷
KR101404728B1 (ko) 2013-02-28 2014-06-09 씨제이제일제당 (주) 스테비오사이드로부터 리바우디오사이드 a를 제조하는 방법
PE20151544A1 (es) 2013-03-15 2015-11-04 Cargill Inc Plantas de stevia con un contenido de rebaudiosido d incrementado
WO2014191581A2 (en) 2013-05-31 2014-12-04 Dsm Ip Assets B.V. Microorganisms for diterpene production
MX2016000452A (es) 2013-07-15 2016-05-12 Dsm Ip Assets Bv Produccion de diterpeno.
MX369591B (es) 2013-07-31 2019-11-13 Dsm Ip Assets Bv Recuperacion de glicosidos de esteviol.
EP3030662B1 (en) 2013-08-07 2020-01-15 Amyris, Inc. Methods for stabilizing production of acetyl-coenzyme a derived compounds
BR112017002783A2 (pt) 2014-08-11 2017-12-19 Evolva Sa produção de glicosídeos de esteviol em hospedeiros recombinantes
PL3183349T3 (pl) 2014-08-19 2021-05-04 Purecircle Sdn Bhd Sposób przygotowania Rebaudiozydu I
MY191735A (en) 2014-09-09 2022-07-13 Evolva Sa Production of steviol glycosides in recombinant hosts
DK3201351T3 (da) 2014-10-03 2021-02-22 Conagen Inc Kaloriefrie sødestoffer og fremgangsmåder til syntetisering
US10463062B2 (en) 2014-11-05 2019-11-05 Manus Bio Inc. Microbial production of steviol glycosides
JP2018503388A (ja) 2015-01-30 2018-02-08 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． 組み換え宿主におけるステビオールグリコシドの生産
CN104726523B (zh) 2015-03-28 2018-08-10 南京工业大学 一种酶法制备莱鲍迪苷m的方法
EP3302097B1 (en) 2015-05-29 2022-03-23 Cargill, Incorporated Heat treatment to produce glycosides
US10815513B2 (en) 2015-05-29 2020-10-27 Cargill, Incorporated Fermentation methods for producing steviol glycosides using high pH and compositions obtained therefrom
WO2017009293A1 (en) 2015-07-10 2017-01-19 Dsm Ip Assets B.V. Steviol glycoside composition
EP3320104B1 (en) 2015-07-10 2021-11-10 DSM IP Assets B.V. Method for preparing a steviol glycoside composition
WO2017025649A1 (en) 2015-08-13 2017-02-16 Dsm Ip Assets B.V. Steviol glycoside transport
WO2017093895A1 (en) 2015-11-30 2017-06-08 Purecircle Sdn Bhd Process for producing high purity steviol glycosides
JP2019503670A (ja) 2015-12-10 2019-02-14 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． 組換え宿主内でのステビオールグリコシドの産生
ES2877475T3 (es) * 2016-03-01 2021-11-16 Univation Tech Llc Método para mejorar la actividad del catalizador ziegler-natta
SG11201808856TA (en) 2016-04-13 2018-11-29 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2017198682A1 (en) 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
AU2017267214A1 (en) 2016-05-16 2018-11-15 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3497109A1 (en) 2016-08-09 2019-06-19 DSM IP Assets B.V. Crystallization of steviol glycosides
ES2906310T3 (es) * 2016-08-12 2022-04-18 Amyris Inc Glicosiltransferasa dependiente de UDP para la producción de alta eficiencia de rebaudiósidos
EP3535406A1 (en) 2016-11-07 2019-09-11 Evolva SA Production of steviol glycosides in recombinant hosts
SG10202112698PA (en) 2017-05-15 2021-12-30 Purecircle Usa Inc High-purity steviol glycosides
MA49836A (fr) * 2017-08-11 2020-06-17 Amyris Inc Kaurène oxydase de pisum sativum pour la production à haut rendement de rebaudiosides
US20200347425A1 (en) 2017-11-20 2020-11-05 Manus Bio, Inc. Microbial host cells for production of steviol glycosides
US20190270971A1 (en) 2018-03-01 2019-09-05 Jason Donald Increasing productivity of microbial host cells that functionally express p450 enzymes
GB201805576D0 (en) 2018-04-04 2018-05-16 Optibiotix Ltd Sweeteners and methods of production thereof
US11608364B2 (en) 2018-04-30 2023-03-21 Dsm Ip Assets B.V. Steviol glycoside transport
JP7584401B2 (ja) 2018-07-30 2024-11-15 テイト アンド ライル ソリューションズ ユー・エス・エー エル・エル・シー 操作されたグリコシルトランスフェラーゼおよびステビオールグリコシドのグルコシル化方法
KR102709594B1 (ko) 2019-06-25 2024-09-26 마누스 바이오, 인크. 우리딘 다이포스페이트-의존성 글라이코실트랜스퍼라제 효소

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WO2018031955A3 (en) 2018-03-15
MY194280A (en) 2022-11-25
US12371677B2 (en) 2025-07-29
MX2019001631A (es) 2019-08-05
JP7404068B2 (ja) 2023-12-25
PT3497222T (pt) 2022-02-09
JP2019524134A (ja) 2019-09-05
US20240174987A1 (en) 2024-05-30
US20210355458A1 (en) 2021-11-18
US20190185826A1 (en) 2019-06-20
JP2022137061A (ja) 2022-09-21
SG11201900930UA (en) 2019-02-27
AU2017308143B2 (en) 2022-04-07
EP3497222A2 (en) 2019-06-19
ES2906310T3 (es) 2022-04-18
US11091743B2 (en) 2021-08-17
EP3497222B1 (en) 2021-12-22
KR20190038612A (ko) 2019-04-08
CN109804073A (zh) 2019-05-24
BR112018074999A2 (pt) 2019-03-12
CN109804073B (zh) 2023-05-16
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