CN109750011A - 一种甘露糖6-磷酸磷酸酶及甘露糖生物制备方法 - Google Patents
一种甘露糖6-磷酸磷酸酶及甘露糖生物制备方法 Download PDFInfo
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- CN109750011A CN109750011A CN201910126884.8A CN201910126884A CN109750011A CN 109750011 A CN109750011 A CN 109750011A CN 201910126884 A CN201910126884 A CN 201910126884A CN 109750011 A CN109750011 A CN 109750011A
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- isomerase
- phosphate
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111793664A (zh) * | 2020-06-19 | 2020-10-20 | 中国科学院天津工业生物技术研究所 | 一种生物制备甘露糖甘油酸的方法 |
CN111808897A (zh) * | 2020-06-19 | 2020-10-23 | 中国科学院天津工业生物技术研究所 | 一种生物制备甘露寡糖的方法 |
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CN114686541A (zh) * | 2022-02-28 | 2022-07-01 | 北京焉支山科技有限公司 | 一种化妆品级己糖-6-磷酸组合物的生物酶法合成方法及其应用 |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6013494A (en) * | 1996-11-13 | 2000-01-11 | E. I. Du Pont De Nemours And Company | Method for the production of 1,3-propanediol by recombinant microorganisms |
CN102382867A (zh) * | 2010-09-06 | 2012-03-21 | 黄卫东 | 一种胞外酶催化糖合成方法 |
WO2018169957A1 (en) * | 2017-03-13 | 2018-09-20 | Bonumose Llc | Enzymatic production of hexoses |
-
2019
- 2019-02-20 CN CN201910126884.8A patent/CN109750011B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6013494A (en) * | 1996-11-13 | 2000-01-11 | E. I. Du Pont De Nemours And Company | Method for the production of 1,3-propanediol by recombinant microorganisms |
CN102382867A (zh) * | 2010-09-06 | 2012-03-21 | 黄卫东 | 一种胞外酶催化糖合成方法 |
WO2018169957A1 (en) * | 2017-03-13 | 2018-09-20 | Bonumose Llc | Enzymatic production of hexoses |
Non-Patent Citations (4)
Title |
---|
ANH DUC NGUYEN等: "Reconstruction of methanol and formate metabolic pathway in non-native host for biosynthesis of chemicals and biofuels", 《BIOTECHNOLOGY AND BIOPROCESS ENGINEERING》 * |
GENBANK: ""Cof-type HAD-IIB family hydrolase [Thermotoga maritima] NCBI Reference Sequence: WP_004081144.1", 《GENBANK》 * |
SCHURMANN, M等: "Fructose-6-phosphate aldolase is a novel class I aldolase from Escherichia coli and is related to a novel group of bacterial transaldolases", 《JOURNAL OF BIOLOGICAL CHEMISTRY》 * |
张勇: "《长三角生物医药发展论坛 首届长三角科技论坛》", 31 October 2004 * |
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