CN109674438A - The adjustable cavity endoscope detection device of object lens and laser scanning cavity endoscope - Google Patents
The adjustable cavity endoscope detection device of object lens and laser scanning cavity endoscope Download PDFInfo
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- CN109674438A CN109674438A CN201910099402.4A CN201910099402A CN109674438A CN 109674438 A CN109674438 A CN 109674438A CN 201910099402 A CN201910099402 A CN 201910099402A CN 109674438 A CN109674438 A CN 109674438A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/00163—Optical arrangements
- A61B1/00188—Optical arrangements with focusing or zooming features
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/04—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances
- A61B1/043—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances for fluorescence imaging
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/06—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements
- A61B1/07—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements using light-conductive means, e.g. optical fibres
Abstract
The embodiment of the present invention provides a kind of adjustable cavity endoscope detection device of object lens and laser scanning cavity endoscope.Wherein, above-mentioned cavity endoscope detection device includes handle housing and detection pipes, relay lens and infinity object lens are provided in the detection channels of detection pipes, and the first optical path of formation and the second optical path, the first optical path include collimation lens, micro electromechanical scanning galvanometer, lens, dichroscope, relay lens and infinity object lens；Second optical path includes infinity object lens, relay lens and dichroscope.Cavity endoscope detection device provided in an embodiment of the present invention and three dimensional non-linear laser scanning cavity endoscope pass through the high-precision zoom motor in detection channels, driving infinity object lens move up and down, pass through zoom operation, to realize the histocyte detection of different depth, obtain the cell structure information of different depth, it is easy to operate, easy to use to meet the detection needs to stomach intestinal tissue and oral cavity tissue etc..
Description
Technical field
The present embodiments relate in laser scanning endoscopic technique field more particularly to a kind of adjustable cavity of object lens
Sight glass detection device and laser scanning cavity endoscope.
Background technique
Gastrointestinal cancer is to induce the second largest reason of developed country crowd cancer stricken death, and in recent years should
Trend is more and more obvious.Surgery radical operation is mainly used for the treatment of gastrointestinal cancer, but is embodied outer
When section's radical operation it needs to be determined that the operation excision specific range, therefore carry out operation consent, it is to be understood that tumour
Whether there is or not Cancer residuals etc. for good pernicious, invasive depth, transfer case and incisxal edge.Therefore biopsy is swollen for gastrointestinal tract under preoperative Gastrointestinal Endoscopes
Tumor tissue diagnosis is a critically important diagnostic evidence.And according to tumorous size, growth position, invasive depth etc., by gastric cancer
Art formula be divided into that stomach is cut entirely, stomach time is cut entirely, gastric resection and endoscopic inferior mucosa or submucous resection etc..
And at present, biopsy carries out iconography imaging usually based on Gastrointestinal Endoscopes supplemented by CT, MRI etc. under Gastrointestinal Endoscopes, or
Assessment is carried out to many gastrointestinal diseases with traditional white light laparoscope or endoscope to deposit.
But based on Gastrointestinal Endoscopes, the imaging of progress iconography haves the shortcomings that some inevitable supplemented by CT, MRI etc.,
For example be easy to cause intestinal tube or knurl bleeding in operation, need artificial drawing or squeeze, when Gastrointestinal Endoscopes cannot pass through intestinal tube,
Endoscopic biopsy is carried out repeatedly so that time delay, if causing severe haemorrhage also needs additional first aid hemostasis etc..And CT, MRI etc.
Complementary detection methods are unable to judge accurately the invasive depth and lymphatic metastasis of upper gastrointestinal road tumour in clinical practice
Situation.And gastroenteric tumor T is judged by stages by endoscopic ultrasonography, its accuracy of document report is only 44.7%~78%, insufficient
To become a reliable diagnostic criteria.Endoscopic ultrasonography is also ineffective to the preoperative judge of local resection operation, can not be accurate
Gastrointestinal mucosa level is segmented, and effect is also poor by stages to N.Therefore, just it is badly in need of one from the point of view of current gastrointestinal tract aided diagnosis technique
The new gastroenteric tumor diagnostic device of kind, with real-time detection stomach intestinal tissue in situ information.
Summary of the invention
For the technical problems in the prior art, the embodiment of the present invention provides peeps in a kind of adjustable cavity of object lens
Mirror detection device and laser scanning cavity endoscope.
In a first aspect, the embodiment of the present invention provides a kind of adjustable cavity endoscope detection device of object lens, comprising:
Handle housing and detection pipes, the handle housing are fixedly connected with the detection pipes, setting in the detection pipes
There are detection channels, relay lens, infinity object lens is provided in the detection channels and for driving the infinity object lens
The zoom motor of lower movement, the infinity object lens and the zoom motor are respectively positioned at the passway of the detection channels, institute
It states the light channel structure being arranged in infinity object lens, the relay lens and the handle housing and forms the first optical path and the second optical path,
Wherein:
First optical path successively includes between the handle housing inner fiber general-purpose interface and the passway
Collimation lens, micro electromechanical scanning galvanometer, the first lens, the second lens, dichroscope, the relay lens and the infinity object
Mirror, wherein first optical path be interfaced to for conducting the received laser signal of the collimation lens from the fiber optic universal it is described
Passway；
Second optical path successively includes the infinity between the passway and the fiber optic universal interface
Object lens, the relay lens and the dichroscope, wherein second optical path is collected for conducting the infinity object lens
Optical signal from the passway to the fiber optic universal interface.
Second aspect, the embodiment of the present invention provide a kind of three dimensional non-linear laser scanning cavity endoscope, comprising:
Phosphor collection device, scanning collection controller, femtosecond pulse laser, fiber coupling module and the present invention are real
Apply the adjustable cavity endoscope detection device of object lens that a first aspect provides, the phosphor collection device and the optical fiber coupling
Molding block is connect with the adjustable cavity endoscope detection device fiber optic communication of the object lens, the phosphor collection device and institute
The adjustable cavity endoscope detection device of object lens is stated to be electrically connected with the scanning collection controller, in which:
The femtosecond pulse laser, for exporting pulsed laser signal to the fiber coupling module；
The fiber coupling module, for coupling the pulsed laser signal of the femtosecond pulse laser output, and
Transmit collimation lens described in the pulsed laser signal to the adjustable cavity endoscope detection device of the object lens；
The adjustable cavity endoscope detection device of object lens exports institute after receiving the pulsed laser signal
The pulsed laser signal autofluorescence substance intracellular to life entity is stated, and described spontaneous by infinity object lens acquisition
The fluorescence signal and second harmonic signal generated after fluorescent material excitation, and export the fluorescence signal and second harmonic letter
Number to the phosphor collection device；
The phosphor collection device converts institute after receiving the fluorescence signal and the second harmonic signal respectively
It states fluorescence signal and the second harmonic signal is corresponding electric signal；
The scanning collection controller sweeps the pulsed laser signal for controlling the micro electromechanical scanning galvanometer
Retouch and synchronous acquisition described in electric signal.
The adjustable cavity endoscope detection device of object lens provided in an embodiment of the present invention and laser scanning cavity endoscope
The first optical path is arranged in the inner space of two component parts in the constituted mode being fixedly connected using handle housing and detection pipes
With the second optical path, to form the adjustable cavity endoscope detection device of hand-held object lens, the first optical path includes collimation lens, liquid
Body lens, micro electromechanical scanning galvanometer, the first lens, the second lens, dichroscope, relay lens and infinity object lens, for passing
Lead the laser signal of excitation autofluorescence substance；Second optical path includes infinity object lens and dichroscope, for collecting double light
Subsignal and second harmonic signal, wherein by the high-precision zoom motor in detection channels, drive infinity object lens or more
It is mobile, the cell structure information of different depth is obtained to realize the histocyte detection of different depth by zoom operation, with
Meet the detection needs to stomach intestinal tissue, oral cavity tissue and uterine cavity inner tissue in human abdominal cavity, easy to operate, user
Just.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is this hair
Bright some embodiments for those of ordinary skill in the art without creative efforts, can be with root
Other attached drawings are obtained according to these attached drawings.
Fig. 1 is the adjustable cavity endoscope detection device structural schematic diagram of object lens that one embodiment of the invention provides；
Fig. 2 be another embodiment of the present invention provides the adjustable cavity endoscope detection device structural schematic diagram of object lens；
Fig. 3 is the adjustable cavity endoscope detection device structural schematic diagram of object lens that yet another embodiment of the invention provides；
Fig. 4 is the adjustable cavity endoscope detection device structural schematic diagram of object lens that further embodiment of this invention provides；
Fig. 5 is the three dimensional non-linear laser scanning cavity endoscope structure schematic diagram that one embodiment of the invention provides；
Fig. 6 be another embodiment of the present invention provides three dimensional non-linear laser scanning cavity endoscope structure schematic diagram；
Fig. 7 is phosphor collection apparatus structure schematic diagram provided in an embodiment of the present invention；
Fig. 8 is the box composite structure for the three dimensional non-linear laser scanning cavity endoscope that one embodiment of the invention provides
Joint sealing structural schematic diagram；
Fig. 9 be another embodiment of the present invention provides three dimensional non-linear laser scanning cavity endoscope box composite structure
Joint sealing structural schematic diagram；
Figure 10 provides the mesa structure schematic diagram of three dimensional non-linear laser scanning cavity endoscope for one embodiment of the invention；
Another embodiment of the present invention provides the mesa structure schematic diagrames of three dimensional non-linear laser scanning cavity endoscope by Figure 11.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art
Every other embodiment obtained without creative efforts, shall fall within the protection scope of the present invention.
At present based on Gastrointestinal Endoscopes, iconography imaging is carried out supplemented by CT, MRI etc. to obtain the good pernicious, leaching of tumour
Moisten depth, transfer case and incisxal edge whether there is or not relevant informations such as Cancer residuals, have the shortcomings that in concrete operations, for example is easy
Lead to intestinal tube or knurl bleeding, need artificial drawing or squeeze, when Gastrointestinal Endoscopes cannot pass through intestinal tube, carries out endoscopic biopsy repeatedly
So that time delay, if causing severe haemorrhage also needs additional first aid hemostasis etc..And the complementary detection methods such as CT, MRI,
The invasive depth and lymphatic metastasis situation of upper gastrointestinal road tumour are unable to judge accurately in clinical practice.And by ultrasound
Mirror judges gastroenteric tumor T by stages, its accuracy of document report is only 44.7%~78%, and being not enough to, which becomes one, reliably examines
Disconnected standard, and endoscopic ultrasonography is ineffective to the preoperative judge of local resection operation, can not precisely subdivided gastrointestinal mucosa level, and
To N, effect is also poor by stages.
And traditional white light laparoscope can assess many gastrointestinal diseases with endoscope, but the technology is only limitted to examine
Survey general form variation.Although being easy to find suspicious region, compared with In vivo detection technology, these technologies and false positive rate
And specificity etc. is related.White light endoscopy is associated with the extensive error that micro-variations diagnose, including ulcerative colitis
Or the inspection diagnosis including Barrett oesophagus and Flat Adenoma depauperation.Confocal endoscope combination laser technology, fluorescence are visited
Survey technology, fast scanning techniques etc. are because that can detect mucous membrane variation in microscopic scale, it is possible to for replacing tissue biopsy, and by
To extensive concern, which has highly sensitive and specificity.But be copolymerized burnt based endoscopic imaging technology still by
As the limitation of depth and fluorescent dye, since stomach and intestine sample has very strong absorption and scattering, imaging depth only to exist visible light
Superficial layer, and it also requires injecting specific fluorescent staining developer, operation is excessively complicated, cannot accurately obtain the leaching of tumour
Moistening depth, transfer case and surgical operation incisxal edge, whether there is or not the relevant informations such as Cancer residual.
And two-photon micro-imaging technique uses the longer femtosecond pulse laser of wavelength to have imaging as excitation light source
The features such as depth is deep, light injury is small, photobleaching region is small, phosphor collection is high-efficient, has in the imaging deep to biological tissue
There is epoch-making meaning.It is micro- that the W.Denk et al. of nineteen ninety Cornell University has developed First two-photon fluorescence in the world
Mirror, using the multi-photon micro-imaging technique based on nonlinear optics and femtosecond pulse.The technology is by utilizing living body
The second harmonic that the autofluorescence and collagen tissue that cell itself generates in tissue generate, can obtain sample real-time, quickly
Institutional framework and cellular morphology.Early in 1986, second harmonic was used for skin research and the research of coronary artery micro-imaging, card
Its real feasibility that be used to observe biological tissue.MPM also can be used as an important tool of cancer research.Cell itself produces
Raw autofluorescence from intracellular nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) (FAD),
It is 460nm that NADH, which issues wavelength, and the secondary oscillation harmonic wave of collagen is 370~390nm, so leading to when observing tumor specimen tissue
Often select the multiphoton microscope of 780~940nm range.MPM imaging is not only suitable with the tumor tissue pathology of standard, simultaneously
The additional information of tumor neogenetic process is also provided, such as can reflect the metabolism of tumor tissue cell by surveying rate value NADH/FAD
It is horizontal.
Using multi-photon imaging technique, multiphoton microscope is capable of providing real-time stomach intestinal tissue's structure and cellular morphology
Learn information.Multi-photon imaging technique has without exogenous marker tissue, extremely sensitive to collagen, small to the light injury of tissue and wear
The features such as depth is deep thoroughly, may be applied to the optical biopsy of gastroenteric tumor.There is presently no useful clinically two-photon abdomens
Hysteroscope and endoscope, and the adjustable cavity endoscope detection device of object lens based on two photon imaging are visited in real time in situ
Survey stomach intestinal tissue's information.
For stomach intestinal tissue's information of real-time detection different depth in situ, the embodiment of the invention provides a kind of object lens can
The cavity endoscope detection device of adjusting, Fig. 1 are the adjustable cavity endoscope detection of object lens that one embodiment of the invention provides
Apparatus structure schematic diagram, as shown in Figure 1, the adjustable cavity endoscope detection device of the object lens includes:
Handle housing 11 and detection pipes 12, the handle housing 11 are fixedly connected with the detection pipes 12, the detection
It is provided with detection channels in pipe, relay lens 117, infinity object lens 118 are provided in the detection channels and for driving
The zoom motor 119 that infinity object lens move up and down is stated, the infinity object lens 118 are respectively positioned on institute with the zoom motor 119
It states at the passway of detection channels, the light being arranged in the infinity object lens 118, the relay lens 117 and the handle housing
Line structure forms the first optical path and the second optical path, in which:
First optical path successively includes between the handle housing inner fiber general-purpose interface 111 and the passway
Collimation lens 112, micro electromechanical scanning galvanometer 113, the first lens 114, the second lens 115, dichroscope 116, the relaying
Mirror 117 and the infinity object lens 118, wherein first optical path is for conducting the received laser of the collimation lens 112
Signal is interfaced to the passway from the fiber optic universal；
Second optical path successively includes the infinity between the passway and the fiber optic universal interface
Object lens 118, the relay lens 117 and the dichroscope 116, wherein second optical path is for conducting the infinity object
The collected optical signal of mirror 118 is from the passway to the fiber optic universal interface.
Specifically, the adjustable cavity endoscope detection device of object lens provided in an embodiment of the present invention is integrated with two light
Road, one is the first optical path for conducting laser signal, which is mainly used for exciting autofluorescence substance；It is another
A is to collect and conduct the two-photon signal and second harmonic signal generated after autofluorescence substance is excited；Wherein, this two
Part light channel structure in optical path includes collimation lens 112, micro electromechanical scanning galvanometer 113, the first lens 114, the second lens
115 and dichroscope 116, it is integrated in handle housing 11, and relay lens 117 and infinity object lens 118 are integrated in detection pipes,
Infinity object lens 118 are arranged at the passway of detection channels, and zoom motor 119, such as voice coil are additionally provided at passway
The high-precisions zoom motor such as motor, for driving infinity object lens to move up and down, by zoom operation, to realize different depth
Histocyte detection, obtains the cell structure information of different depth, wherein infinity object lens are mountable in high-precision zoom motor
It is interior.
Wherein relay lens 117 is set in detection channels on the inside of the object lens of infinity, and relay lens 117 for passing over long distances
The collected two-photon signal of infinity object lens and second harmonic signal are led to dichroscope, the image planes of infinity object lens 118
It is overlapped with the focal plane of relay lens 117, the effect of relay lens is that will pass through the laser signal of micro electromechanical scanning mirror for passing picture first
Scanning area is with ratio of 1:1 etc. than conducting to the image planes of infinity object lens 118.Secondly relay lens can also be by infinity object lens
The light signal transduction being collected into is collected to the intracorporal light channel structure part of handle case via the fiber optic bundle of light channel structure,
In, dichroscope 116 can lead to short anti-dichroscope or short elongated anti-dichroscope for length, i.e. setting length leads to short anti-dichroscope
When, transmit the pulsed laser signal for exciting autofluorescence substance, the two-photon signal and second harmonic that reflecting and collecting arrives
Signal, as shown in Figure 1, at this point, the adjustable cavity endoscope detection device of the object lens can be the detection device of laparoscope；Fig. 2
For another embodiment of the present invention provides the adjustable cavity endoscope detection device structural schematic diagram of object lens, as shown in Fig. 2, working as
When the dichroscope 116 is short elongated anti-dichroscope, the pulsed laser signal for exciting autofluorescence substance is reflected, is transmitted
The two-photon signal and second harmonic signal being collected into, the dichroscope reflect the process being emitted from fiber optic universal interface 111
It is incident on dichroscope 116 after collimation lens 112, micro electromechanical scanning galvanometer 113, the first lens 114, the second lens 115
Laser signal transmits the collected two-photon signal of infinity object lens 118 and second harmonic signal to infinity object lens 118,
At this point, the adjustable cavity endoscope detection device of the object lens can be the detection device of mouth mirror, the wherein detection of the mouth mirror
Device also includes 12 two parts of handle housing 11 and detection pipes.
The adjustable cavity endoscope detection device of object lens provided in an embodiment of the present invention uses handle housing and detection
The first optical path and the second optical path is arranged in the inner space of two component parts, to form hand in the constituted mode that pipe is fixedly connected
The adjustable cavity endoscope detection device of the object lens held, the first optical path include collimation lens, liquid lens, micro electromechanical scanning vibration
Mirror, the first lens, the second lens, dichroscope, relay lens and infinity object lens, for conducting excitation autofluorescence substance
Laser signal；Second optical path includes infinity object lens and dichroscope, for collecting two-photon signal and second harmonic letter
Number, wherein by the high-precision zoom motor in detection channels, infinity object lens is driven to move up and down, by zoom operation, with
The histocyte detection for realizing different depth, obtains the cell structure information of different depth, to meet to stomach and intestine in human abdominal cavity
The detection of road tissue, oral cavity tissue and uterine cavity inner tissue needs, easy to operate, easy to use.
On the basis of the various embodiments described above, the adjustable cavity endoscope detection dress of object lens provided in an embodiment of the present invention
Light channel structure in setting further includes liquid lens, is peeped in the adjustable cavity of object lens that Fig. 3 provides for yet another embodiment of the invention
Mirror detection device structural schematic diagram, as shown in figure 3, liquid lens 110 is located at collimation lens 112 and micro electromechanical scanning galvanometer 113
Between, to form the first new optical path, the first new optical path successively includes between fiber optic universal interface 111 and passway
Collimation lens 112, liquid lens 110, micro electromechanical scanning galvanometer 113, the first lens 114, the second lens 115, dichroscope 116
And infinity object lens 118.I.e. liquid lens setting so that, can by liquid lens apply voltage or current make liquid
110 surface of lens generates the bending accordingly arrived, and then the directional light that collimation lens 112 are emitted generates different focal powers.Tool
Body optical path are as follows: laser signal is incident on liquid lens from fiber exit in parallel after collimation lens, from liquid lens 110
Generate corresponding focal power according to the voltage or current signal of load, outgoing be converging or diverging with light by micro electromechanical scanning galvanometer,
First lens, the second lens, dichroscope converge on sample after relay lens is transmitted to infinity object lens.Wherein, liquid
The focus for the laser signal that the power variation that lens introduce can be such that infinity object lens mouth is emitted is moved forward and backward in longitudinal direction,
And the response speed of liquid lens is very fast, sweeping for quick longitudinal direction may be implemented in KHz magnitude in scan frequency
Retouch imaging.Wherein, liquid lens is equivalent to parallel plate glass when not applying voltage or current signal, unglazed to laser signal
Focal power and the focus after the object lens of infinity will not be made to generate any offset, to realize three-dimensional imaging.Specifically used
When, the liquid lens is complementary with zoom motor 119, infinity object lens position is adjusted by zoom motor 119, in coarse adjustment to accordingly
Behind depth position, system is switched to liquid lens zoom scan mode, quick three-dimensional imaging is carried out to sample, wherein working as cavity
Endoscope detection device can also carry out zoom adjustment when not installing zoom motor 119, only by liquid lens.
On the basis of the various embodiments described above, the adjustable cavity endoscope detection dress of object lens provided in an embodiment of the present invention
Several illumination channels are additionally provided in the detection pipes set, Fig. 4 is the adjustable chamber of object lens that further embodiment of this invention provides
Internal sight glass detection device structural schematic diagram is used for transmission illumination optical signal as shown in figure 4, being provided in illumination channel 123
Illumination fiber optic bundle, wherein illumination channel 123 is uniformly distributed centered on the axle center of detection channels.It is i.e. provided in an embodiment of the present invention
Several illumination channels 123, the illumination channel are additionally provided in detection pipes in the adjustable cavity endoscope detection device of object lens
123 more than one are both provided with illumination fiber optic bundle in each channel, and lighting fiber has certain pore size angle, and not needing lens can
It to be directly used in divergent illumination, and illuminates channel 123 and is uniformly distributed centered on the axle center of detection channels, be that object lens are adjustable
Cavity endoscope detection device provides Uniform Illumination, to facilitate the test serum zone state before work observation infinity object lens.
On the basis of the various embodiments described above, the adjustable cavity endoscope detection dress of object lens provided in an embodiment of the present invention
Observation channel is additionally provided in the detection pipes set, as shown in figure 4, observation channel is located between detection channels and illumination channel,
Wherein:
It observes at the passway in channel and is provided with observation camera lens 124, the light field optical fiber in observation camera lens 124 and observation channel
Beam is connected, to obtain the test serum regional image information before the object lens of infinity.Object lens i.e. provided in an embodiment of the present invention
Observation channel is additionally provided in detection pipes in adjustable cavity endoscope detection device, which is located at detection channels
Illumination channel between, and be provided with observation camera lens 124 and light field fiber optic bundle, light field fiber optic bundle be imaging optical fiber bundle, be used for
Test serum regional image information before the infinity object lens that transmitting observation camera lens 124 captures, wherein observation channel can be
One, or three-dimensional light field cavity endoscope function is realized in two formation binocular observations.
On the basis of the various embodiments described above, the adjustable cavity endoscope detection dress of object lens provided in an embodiment of the present invention
It is additionally provided with sorption channel in the detection pipes set, as shown in figure 4, sorption channel 125 is located at illumination channel and detection tube edges
Between.Use is additionally provided in detection pipes in the adjustable cavity endoscope detection device of object lens i.e. provided in an embodiment of the present invention
In making the adjustable cavity endoscope detection device of object lens be adsorbed on the sorption channel 125 in test serum, adsorbed by extracting
Air in channel 125 forms negative pressure in sorption channel 125, so that the adjustable cavity endoscope detection device of object lens is inhaled
It is attached in test serum, wherein sorption channel 125 is located between illumination channel and detection tube edges, that is, is located at outside illumination channel
Side, at the position of detection pipes avris.
On the basis of the various embodiments described above, the adjustable cavity endoscope detection dress of object lens provided in an embodiment of the present invention
Button hole is offered on the handle housing set, and switching push button is provided in button hole, switching push button is for switching different filters
Mating plate, to obtain the illumination optical signal of different wave length.The adjustable cavity endoscope of object lens i.e. provided in an embodiment of the present invention is visited
It is provided with switching push button in the button hole for the handle housing surveyed in device, it can be with switch filtering different wave length by the switching push button
The optical filter of optical signal is illuminated, so that staff can choose the illumination optical signal transmitted through the different wave length come, wherein cutting
The function of changing button can also be customized by software, modifies its corresponding function.
On the basis of the various embodiments described above, the adjustable cavity endoscope detection dress of object lens provided in an embodiment of the present invention
Imaged button is additionally provided in the button hole set, imaged button is for controlling the image-forming module pair being connected with light field fiber optic bundle
Test serum region before the object lens of infinity is imaged.The adjustable cavity endoscope of object lens i.e. provided in an embodiment of the present invention
It is provided with imaged button in the button hole of handle housing in detection device, can control and light field optical fiber by the imaged button
The image-forming module that beam is connected carries out imaging of taking pictures to the test serum region before the object lens of infinity, wherein the function of imaged button
Can also be customized by software, modify its corresponding function.
On the basis of the various embodiments described above, the adjustable cavity endoscope detection dress of object lens provided in an embodiment of the present invention
Sorption channel in setting is a circular passage or several circular channels.I.e. for making the adjustable cavity endoscope detection dress of object lens
Setting the sorption channel being adsorbed in test serum can be for one along the circular passage on the inside of detection pipes, or Duo Geyuan
Shape channel, to form sufficiently large negative pressure, so that the adjustable cavity endoscope detection device of object lens is adsorbed in test serum.
The embodiment of the present invention also provides a kind of three dimensional non-linear laser scanning cavity endoscope, and Fig. 5 is that the present invention one is implemented
The three dimensional non-linear laser scanning cavity endoscope structure schematic diagram that example provides, as shown in figure 5, the three dimensional non-linear laser scanning
Cavity endoscope includes:
Phosphor collection device 56, scanning collection controller 531, femtosecond pulse laser, fiber coupling module and above-mentioned
The adjustable cavity endoscope detection device 1 of object lens that each embodiment provides, phosphor collection device 56 and fiber coupling module are equal
It is connect with adjustable 1 fiber optic communication of cavity endoscope detection device of object lens, phosphor collection device 56 and the adjustable chamber of object lens
Internal sight glass detection device 1 is electrically connected with scanning collection controller 531, in which:
Femtosecond pulse laser, for exporting pulsed laser signal to fiber coupling module；
Fiber coupling module, for coupling the pulsed laser signal of femtosecond pulse laser output, and transmission pulse laser
Signal collimation lens into the adjustable cavity endoscope detection device 1 of object lens；
The adjustable cavity endoscope detection device 1 of object lens, after receiving pulsed laser signal, output pulse laser letter
Number autofluorescence substance intracellular to life entity, and obtained by infinity object lens and to be generated after the excitation of autofluorescence substance
Fluorescence signal and second harmonic signal, and fluorescence signal and second harmonic signal are exported to phosphor collection device 56；
Phosphor collection device 56, after receiving fluorescence signal and second harmonic signal, difference conversion fluorescence signal and two
Rd harmonic signal is corresponding electric signal；
Scanning collection controller 531 is scanned pulsed laser signal for controlling micro electromechanical scanning galvanometer, Yi Jitong
Step acquisition electric signal.
Specifically, three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention includes phosphor collection device
56, scanning collection controller 531, femtosecond pulse laser, fiber coupling module and the adjustable cavity endoscope of object lens are visited
Device 1 is surveyed, so that it is non-to form the three-dimensional detected using two photon imaging technology to human body gastrointestinal tissue and oral cavity tissue
Linear laser scans cavity endoscope, wherein femtosecond pulse laser can be used for exciting human intestines with emission pulse laser signal
Autofluorescence substance in gastric tissue and oral tissue cell generates multiphoton fluorescence signal and second harmonic signal, including
Using the FAD and collagen in the femtosecond pulse laser activated cell of 920nm, excite 500-600nm fluorescence signal and
The second harmonic signal of 460nm, and pass through 780nm femtosecond pulse laser activated cell in FAD or NADH etc. it is spontaneous
Fluorescent material, to generate corresponding fluorescence signal and second harmonic signal, wherein femtosecond pulse laser and fiber coupling module
It is grouped together into laser emitting module 540；
Wherein, phosphor collection device 56 is integrated with two paths of signals and collects optical path, respectively fluorescence signal collection optical path and two
Rd harmonic signal collects optical path, the collection respectively of Lai Shixian fluorescence signal and second harmonic signal；Scanning collection controller 531 is controlled
Micro electromechanical scanning galvanometer processed is scanned pulsed laser signal and autofluorescence substance is excited to generate fluorescence signal and secondary humorous
Wave signal, and the first electric signal and second that acquisition 56 conversion fluorescence signal of phosphor collection device and second harmonic signal obtain
Electric signal；The three dimensional non-linear laser scanning cavity endoscope is according to adjustable 1 structure of cavity endoscope detection device of object lens
Difference, laparoscope and mouth mirror can be divided into.Wherein, the resolution ratio of the three dimensional non-linear laser scanning cavity endoscope is settable
For 800nm, visual field can be 400 microns * 400 microns, and image taking speed can be 26 frames (256*256 pixel) or 13 frame (512*
512 pixels).
Three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention is adopted using phosphor collection device, scanning
Collect controller, femtosecond pulse laser, fiber coupling module and the adjustable cavity endoscope detection device of object lens, thus shape
At the three dimensional non-linear laser scanning chamber detected using two photon imaging technology to human body gastrointestinal tissue and oral cavity tissue
Internal sight glass adjusts infinity objective focal length by liquid lens, realizes the 3-D scanning of laser scanning microscope, pass through femtosecond
Autofluorescence substance obtains multiphoton fluorescence signal and second harmonic signal in pulse laser activated cell, realizes laser scanning
Microscope is non-linear, collects fluorescence signal and second harmonic signal by phosphor collection device, and be converted to corresponding telecommunications
Number, and then the corresponding fluorescent image etc. for reflecting cell tissue structure is obtained by the electric signal, wherein the adjustable chamber of object lens
The use of internal sight glass detection device allow staff flexibly to gastrointestinal tissue in human abdominal cavity, oral cavity tissue and
Uterine cavity inner tissue is detected, and when detecting to human body gastrointestinal tissue, only need to open up an osculum to human abdomen
The pain for so reducing operation cost and patient can carry out nothing by nature channel (vagina) when uterine cavity inner tissue is imaged
Wound detection, equipment operation are simple, easy to use.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror further includes lighting module and image-forming module, as shown in figure 5, lighting module 534 is adjustable with object lens with image-forming module 533
The cavity endoscope detection device fiber optic communication of section connects, in which:
Lighting module 534 successively includes illuminating lens 5342, variable filter 5341 and lighting source 5343, illuminating lens
5342 are connected with illumination fiber optic bundle, and lighting source is for providing illumination optical signal；
Image-forming module 533 successively includes imaging len 5331 and camera 5332, imaging len 5331 and light field fiber optic bundle phase
Connection, camera 5332 is for obtaining test serum regional image information.Three dimensional non-linear laser i.e. provided in an embodiment of the present invention
Scanning cavity endoscope is additionally provided with lighting module 534 and image-forming module 533, wherein lighting module 534 successively includes illumination
Lens 5342, variable filter 5341 and lighting source 5343, wherein lighting source passes through electric variable optical filter runner, can
To switch different optical filters, to obtain the illumination optical signal of different wave length, basic principle is not interfere two-photon fluorescence imaging,
Such as obtain autofluorescence and when second harmonic, red or infrared optical filter can be switched to, with obtain 370nm,
The illumination optical signal of 635nm or infrared 850nm, 940nm, illumination optical signal enter illumination fiber optic bundle by Lens Coupling；
Image-forming module 533 includes successively imaging len and camera, and lens focus is imaged on camera, bright for directly observing
?.Wherein camera is two corresponding with binocular light field fiber optic bundle, and light field imaging and two photon imaging form multi-modal laparoscope, bright
Field binocular three-dimensional stereo laparoscope mode, carries out wide-field sample view, the basic pattern of main detection sample.It can for having
Doubtful or interested region, can switch to two-photon mode, carries out autofluorescence and Second Harmonic Imaging, observes the cell of sample
Grade form provides foundation for further judgement.Wherein camera can set for the imaging based on image devices such as CCD or CMOS
It is standby.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror further includes air extractor, as shown in figure 5, air extractor 52 includes mainly aspiration pump, passes through exhaust pipe and sorption channel phase
Even, extraction valve is set in exhaust pipe, extraction valve is electrically connected with air extractor 52, and air extractor 52 is opened by adjusting extraction valve
The size closed and be opened and closed, controls the extraction flow of exhaust pipe, to realize that the pumping to sorption channel controls, and then adjusts
Negative pressure in sorption channel, so that the adjustable cavity endoscope detection device of object lens is adsorbed on people by the effect of atmospheric pressure
The tissue such as body stomach or oral cavity reduces bio-tissue activity bring motion artifacts, so that imaging is more stable, clear.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror further includes industrial personal computer, as shown in figure 5, industrial personal computer 532 is electrically connected with scanning collection controller 531, in which:
Industrial personal computer 532 is for obtaining collected first electric signal of scanning collection controller 531 and the second electric signal, and base
The first fluorescent image is generated in the first electric signal and the second fluorescent image is generated based on the second electric signal.That is the embodiment of the present invention
The three dimensional non-linear laser scanning cavity endoscope of offer further includes the industrial personal computer 532 being electrically connected with scanning collection controller 531,
The industrial personal computer 532 is based on the first electric signal and generates the first fluorescent image and generate the second fluorescent image based on the second electric signal,
It can be respectively used to display eucaryotic cell structure and fibre structure information, control software is wherein installed on industrial personal computer, by controlling software,
Control instruction is sent to scanner, to control scanning collection controller, to obtain above-mentioned first electric signal and the second electric signal.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror further includes display, as shown in figure 5, display 55 is electrically connected with industrial personal computer 532, for showing the first fluorescent image and the
Two fluorescent images.Three dimensional non-linear laser scanning cavity endoscope i.e. provided in an embodiment of the present invention further includes for showing first
The display 55 of fluorescent image and the second fluorescent image, by display 55, staff can directly acquire the first fluorogram
The relevant information of picture and the second fluorescent image.
Wherein, Fig. 6 be another embodiment of the present invention provides three dimensional non-linear laser scanning cavity endoscope structure signal
Figure, as shown in fig. 6, the three dimensional non-linear laser scanning cavity endoscope also includes:
Phosphor collection device 56, scanning collection controller 531, femtosecond pulse laser, fiber coupling module and above-mentioned
The adjustable cavity endoscope detection device 1 of object lens of each embodiment offer, air extractor 52, industrial personal computer 532, lighting module
534, image-forming module 533, phosphor collection device 56 and fiber coupling module are filled with the adjustable cavity endoscope detection of object lens
Set 1 fiber optic communication connection, phosphor collection device 56 and the adjustable cavity endoscope detection device 1 of object lens with scanning collection control
Device 531 processed is electrically connected, wherein each part mentioned above module or the function of device and the apparatus function effect phase in the various embodiments described above
Together, including femtosecond pulse laser and fiber coupling module are grouped together into laser emitting module 540, lighting module 534
It successively include illuminating lens 5342, variable filter 5341 and lighting source 5343, image-forming module 533 successively includes imaging len
And camera, the adjustable cavity endoscope detection device 1 of object lens in the three dimensional non-linear laser scanning cavity endoscope are mouth
Hysteroscope detection device contains liquid lens in the light channel structure of the adjustable cavity endoscope detection device of the object lens, makees
With identical as the liquid lens for including in the various embodiments described above, optical path is also identical with optical path corresponding in the various embodiments described above.
On the basis of the various embodiments described above, Fig. 7 is phosphor collection apparatus structure schematic diagram provided in an embodiment of the present invention,
As shown in fig. 7, phosphor collection device provided in an embodiment of the present invention includes collecting fiber optic universal interface 881, the first photomultiplier transit
Pipe 882, the second photomultiplier tube 883 and positioned at collect between fiber optic universal interface 881 and the first photomultiplier tube 882 the
One collects optical path, collects optical path positioned at second collected between fiber optic universal interface 881 and the second photomultiplier tube 883, in which:
First collection optical path successively includes coupling collecting lens 81, infrared fileter 82, the filter of the first dichroscope 83, first
Mating plate 84 and the first collecting lens 85, wherein the first collection optical path is used to collect the fluorescence letter that phosphor collection device receives
Number, the first photomultiplier tube 882 is the first electric signal for conversion fluorescence signal；
Second collection optical path successively includes coupling collecting lens 81, infrared fileter 82, the first dichroscope the 83, the 2nd 2
To Look mirror 86, the second optical filter 87 and the second collecting lens 88, wherein the second collection optical path is for collecting phosphor collection device
The second harmonic signal received, the second photomultiplier tube 883 is for converting second harmonic signal as the second electric signal.That is this hair
The phosphor collection device that bright embodiment provides has simple two-way signal collecting function, is integrated with two-way optical path, wherein first collects light
The first dichroscope 83 in road is transmission fluorescence signal, the dichroscope of reflected second harmonics, the second dichroscope 86 and the
One dichroscope 83 is same dichroscope, is used for reflected second harmonics, and the first optical filter 84 is filtered for transmiting fluorescence signal
Except remaining interference signal, the second optical filter 87 filters out remaining interference signal for transmiting corresponding second harmonic signal, for example,
When using autofluorescence substance in the femto second optical fiber laser exciting human abdominal cavity 780nm or Stomatocyte, it can be obtained 390nm's
The two-photon auto flourescence signals of second harmonic signal and 450-600nm, are passed through by 420nm above wavelength, 420 or less wavelength
The dichroscope of reflection i.e. the first dichroscope 83 can separate two-way fluorescence, use the first optical filter of 390 ± 20nm respectively
The second optical filter 87 available clean second harmonic signal and fluorescence signal of 84 and 450-600nm.
Wherein, Fig. 8 is the box combination for the three dimensional non-linear laser scanning cavity endoscope that one embodiment of the invention provides
The joint sealing structural schematic diagram of structure, as shown in figure 8, it is integrated to be integrated in display 55 and the cabinet for being equipped with modules on case lid
Together, facilitate whole equipment mobile, and replacement workplace, and the display 55 is when in use, case can be placed in addition
On body, to facilitate staff to obtain the information on display, wherein in the three dimensional non-linear laser scanning cavity endoscope
The adjustable cavity endoscope detection device 1 of object lens is mouth mirror detection device.When having used the three dimensional non-linear laser scanning
After cavity endoscope, staff can portable equipment case, convenient changing workplace, especially in hospital, laboratory or outdoor field
Institute, can be more convenient using the equipment.
Wherein, Fig. 9 be another embodiment of the present invention provides box group of three dimensional non-linear laser scanning cavity endoscope
The joint sealing structural schematic diagram of structure is closed, as shown in figure 9, being integrated in display 55 and the cabinet collection for being equipped with modules on case lid
At, facilitating whole equipment mobile together, and replacement workplace, and the display 55 is when in use, can other than be placed on
On cabinet, to facilitate staff to obtain the information on display, wherein in the three dimensional non-linear laser scanning cavity endoscope
The adjustable cavity endoscope detection device 1 of object lens be laparoscope detection device, and laparoscope detection device can be set simultaneously
It sets multiple.After having used the three dimensional non-linear laser scanning cavity endoscope, staff can portable equipment case, convenient changing
It workplace can be more convenient using the equipment especially in hospital, laboratory or outdoor location.
On the basis of the various embodiments described above, three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention
In the adjustable cavity endoscope detection device of object lens be it is multiple.Phosphor collection device and light i.e. provided in an embodiment of the present invention
Fine coupling module can be connect with the adjustable cavity endoscope detection device fiber optic communication of multiple object lens simultaneously, i.e., in a three-dimensional
Multiple detection devices are integrated in non-linear laser scanning cavity endoscopic system, while to realize to gastrointestinal tissue different parts
Detection, to compare and analyze.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror further includes adjusting optical fiber, for phosphor collection device and fiber coupling module respectively with the adjustable cavity endoscope of object lens
Optical fiber between detection device transmits connection, in which:
Adjust the adjustable in length of optical fiber.Three dimensional non-linear laser scanning cavity endoscope i.e. provided in an embodiment of the present invention
In phosphor collection device and fiber coupling module pass through in length-adjustable adjusting optical fiber and the adjustable cavity of object lens respectively
Sight glass detection device carries out optical fiber transmission connection and carries out flexible movement detector to realize according to different experiments scene needs,
Avoid the limitation of limited fiber lengths, wherein the adjustable in length of optical fiber is adjusted, to pass through the optical fiber for replacing different length, reality
The application of existing various occasions can carry out the optical fiber replacement of different length at any time as needed.
For the three dimensional non-linear laser scanning cavity endoscope that the various embodiments described above provide, the embodiment of the present invention is also provided
Another specific embodiment, Figure 10 provide three dimensional non-linear laser scanning cavity endoscope for one embodiment of the invention
Mesa structure schematic diagram, as shown in Figure 10, the three dimensional non-linear laser scanning cavity endoscope include that air extractor 52, first fills
Set 53, second device 54, display 55 and the adjustable cavity endoscope detection device 1 of object lens, wherein in first device 53
It is integrated with scanning collection controller and industrial personal computer, industrial personal computer is electrically connected with display 55, and second device 54 is integrated with femtosecond pulse
Laser, fiber coupling module and phosphor collection device, lighting module and image-forming module, fiber coupling module and phosphor collection
Device is transmitted with 51 optical fiber of absorption type microscope detection device and is connected, wherein the adjustable cavity endoscope detection dress of object lens
1 is set for mouth mirror detection device, for detecting human oral cavity tissue, to understand good pernicious, invasive depth, the transfer case of tumour
And whether there is or not the information such as Cancer residual, the absorption type three dimensional non-linear laser scanning microscope working principle and above-mentioned each implementations for incisxal edge
Example is identical, and details are not described herein again.
Wherein, another embodiment of the present invention provides the mesa structures of three dimensional non-linear laser scanning cavity endoscope to show by Figure 11
It is intended to, as shown in figure 11, which also includes air extractor 52, first device 53, the
Two devices 54, display 55 and the adjustable cavity endoscope detection device 1 of object lens, wherein be integrated in first device 53
Scanning collection controller and industrial personal computer, industrial personal computer are electrically connected with display 55, second device 54 be integrated with femtosecond pulse laser,
Fiber coupling module and phosphor collection device, lighting module and image-forming module, fiber coupling module and phosphor collection device are equal
It transmits and connects with 51 optical fiber of absorption type microscope detection device, wherein the adjustable cavity endoscope detection device 1 of object lens is abdomen
Hysteroscope detection device, the laparoscope detection device are embedded in human abdomen, detect to gastrointestinal tissue, to understand the good evil of tumour
Whether there is or not the information such as Cancer residual for property, invasive depth, transfer case and incisxal edge.
Above-mentioned, although the foregoing specific embodiments of the present invention is described with reference to the accompanying drawings, not to invention protection scope
Limitation, those skilled in the art should understand that, based on the technical solutions of the present invention, those skilled in the art are not required to
The above is only preferred embodiment of the present application, are not intended to restrict the invention, for those skilled in the art, this
Application can have various modifications and variations.Within the spirit and principles of this application, made any modification, equivalent replacement,
Improve etc., it should be included within the scope of protection of this application.
The apparatus embodiments described above are merely exemplary, wherein unit can be as illustrated by the separation member
Or may not be and be physically separated, component shown as a unit may or may not be physical unit, i.e.,
It can be located in one place, or may be distributed over multiple network units.It can select according to the actual needs therein
Some or all of the modules achieves the purpose of the solution of this embodiment.Those of ordinary skill in the art are not paying creative labor
In the case where dynamic, it can understand and implement.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: it still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features；
And these are modified or replaceed, technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution spirit and
Range.
Claims (10)
1. a kind of adjustable cavity endoscope detection device of object lens characterized by comprising
Handle housing and detection pipes, the handle housing are fixedly connected with the detection pipes, and spy is provided in the detection pipes
Channel is surveyed, relay lens, infinity object lens is provided in the detection channels and is moved down on the infinity object lens for driving
Dynamic zoom motor, the infinity object lens and the zoom motor are respectively positioned at the passway of the detection channels, the nothing
The light channel structure being arranged in poor remote object lens, the relay lens and the handle housing forms the first optical path and the second optical path, in which:
First optical path successively includes the collimation between the handle housing inner fiber general-purpose interface and the passway
Lens, micro electromechanical scanning galvanometer, the first lens, the second lens, dichroscope, the relay lens and the infinity object lens,
Wherein first optical path is interfaced to described lead to from the fiber optic universal for conducting the received laser signal of the collimation lens
Road junction；
Second optical path successively include infinity object lens between the passway and the fiber optic universal interface,
The relay lens and the dichroscope, wherein second optical path is for conducting the collected light letter of the infinity object lens
Number from the passway to the fiber optic universal interface.
2. the adjustable cavity endoscope detection device of object lens according to claim 1, which is characterized in that the optical path knot
Structure further includes liquid lens, and the liquid lens is between the collimation lens and the micro electromechanical scanning galvanometer, to be formed
The first new optical path, the first new optical path successively include the standard between the fiber optic universal interface and the passway
Straight lens, the liquid lens, the micro electromechanical scanning galvanometer, first lens, second lens, the dichroscope
And the infinity object lens.
3. the adjustable cavity endoscope detection device of object lens according to claim 1 or 2, which is characterized in that the spy
It is additionally provided with several illumination channels in test tube, is provided with the lighting fiber for being used for transmission illumination optical signal in the illumination channel
Beam, wherein the illumination channel is uniformly distributed centered on the axle center of the detection channels.
4. the adjustable cavity endoscope detection device of object lens according to claim 3, which is characterized in that the detection pipes
It is inside additionally provided with observation channel, the observation channel is located between the detection channels and the illumination channel, in which:
Observation camera lens, the bright field light in the observation camera lens and the observation channel are provided at the passway in the observation channel
Fine beam is connected, to obtain the test serum regional image information before the infinity object lens.
5. the adjustable cavity endoscope detection device of object lens according to claim 3, which is characterized in that the detection pipes
It is inside additionally provided with sorption channel, the sorption channel is between the illumination channel and the detection tube edges.
6. the adjustable cavity endoscope detection device of object lens according to claim 4, which is characterized in that the handle case
Button hole is offered on body, and switching push button is provided in the button hole, the switching push button is used to switch different optical filters,
To obtain the illumination optical signal of different wave length.
7. the adjustable cavity endoscope detection device of object lens according to claim 6, which is characterized in that the button hole
It is inside additionally provided with imaged button, the imaged button is for controlling the image-forming module being connected with the light field fiber optic bundle to described
Test serum region before the object lens of infinity is imaged.
8. a kind of three dimensional non-linear laser scanning cavity endoscope characterized by comprising
Phosphor collection device, scanning collection controller, femtosecond pulse laser, fiber coupling module and claim 1-7 appoint
Cavity endoscope detection device described in one, the phosphor collection device and the fiber coupling module are and in the cavity
Sight glass detection device fiber optic communication connection, the phosphor collection device and the cavity endoscope detection device with the scanning
Acquisition controller electrical connection, in which:
The femtosecond pulse laser, for exporting pulsed laser signal to the fiber coupling module；
The fiber coupling module for coupling the pulsed laser signal of the femtosecond pulse laser output, and transmits
Collimation lens described in the pulsed laser signal to the cavity endoscope detection device；
The cavity endoscope detection device exports the pulsed laser signal extremely after receiving the pulsed laser signal
The intracellular autofluorescence substance of life entity, and produced after obtaining the autofluorescence substance excitation by the infinity object lens
Raw fluorescence signal and second harmonic signal, and the fluorescence signal and the second harmonic signal are exported to the phosphor collection
Device；
The phosphor collection device is converted described glimmering respectively after receiving the fluorescence signal and the second harmonic signal
Optical signal and the second harmonic signal are corresponding electric signal；
The scanning collection controller is scanned the pulsed laser signal for controlling the micro electromechanical scanning galvanometer,
And electric signal described in synchronous acquisition.
9. three dimensional non-linear laser scanning cavity endoscope according to claim 8, which is characterized in that further include illumination mould
Block and image-forming module, the lighting module and the image-forming module connect with the cavity endoscope detection device fiber optic communication
It connects, in which:
The lighting module successively includes illuminating lens, variable filter and lighting source, the illuminating lens and lighting fiber
Beam is connected, and the lighting source is for providing illumination optical signal；
The image-forming module successively includes imaging len and camera, and the imaging len is connected with light field fiber optic bundle, the phase
Machine is for obtaining test serum regional image information.
10. three dimensional non-linear laser scanning cavity endoscope according to claim 8, which is characterized in that the fluorescence is received
Acquisition means include collecting fiber optic universal interface, the first photomultiplier tube, the second photomultiplier tube and being located at the collection optical fiber
First between general-purpose interface and first photomultiplier tube collects optical path, is located at the collection fiber optic universal interface and described
Second between second photomultiplier tube collects optical path, in which:
It is described first collection optical path successively include coupling collecting lens, infrared fileter, the first dichroscope, the first optical filter with
And first collecting lens, wherein the first collection optical path for collecting the fluorescence signal that phosphor collection device receives,
First photomultiplier tube is for converting the fluorescence signal as the first electric signal；
It is described second collection optical path successively include the coupling collecting lens, the infrared fileter, first dichroscope,
Second dichroscope, the second optical filter and the second collecting lens, wherein the second collection optical path is for collecting phosphor collection
The second harmonic signal that device receives, second photomultiplier tube is for converting the second harmonic signal as second
Electric signal.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113467071A (en) * | 2021-07-27 | 2021-10-01 | 郑州光超医疗科技有限公司 | Focusable in-vivo tissue high-resolution optical scanning probe |
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