CN210055953U

CN210055953U - Variable-focus cavity endoscope detection device and laser scanning cavity endoscope

Info

Publication number: CN210055953U
Application number: CN201920180627.8U
Authority: CN
Inventors: 吴润龙; 王爱民; 江文茂; 吴丹磊; 胡炎辉; 程和平
Original assignee: Beijing Chaowei Landscape Biology Technology Co Ltd
Current assignee: Beijing Chaowei Landscape Biology Technology Co Ltd
Priority date: 2019-01-31
Filing date: 2019-01-31
Publication date: 2020-02-14
Anticipated expiration: 2029-01-31

Abstract

An embodiment of the utility model provides a formula cavity endoscope detection device and laser scanning cavity endoscope can zoom. The variable-focus cavity endoscope detection device comprises an outer fixing shell and an inner clamping device, wherein a variable-focus motor is arranged in the outer fixing shell, the inner clamping device is driven by the variable-focus motor to move up and down relative to the outer fixing shell, a first light path for receiving and transmitting an output pulse laser signal to an autofluorescence substance in a body cavity cell of a living body to be detected and a second light path for collecting and transmitting a fluorescence signal and a second harmonic signal generated by exciting the autofluorescence substance are arranged in the inner clamping device. The embodiment of the utility model provides a formula cavity endoscope detection device and laser scanning cavity endoscope that can zoom realizes the relative movement of interior clamping device and external fixation casing through the motor that zooms, makes formula cavity endoscope detection device that can zoom the operation, acquires the cell imaging of the different degree of depth such as human abdominal cavity stomach tissue or oral cavity, palace chamber tissue, easy operation, convenient to use.

Description

Variable-focus cavity endoscope detection device and laser scanning cavity endoscope

Technical Field

The embodiment of the utility model provides a relate to laser scanning endoscope technical field, especially relate to a formula cavity endoscope detection device and laser scanning cavity endoscope can zoom.

Background

Gastrointestinal malignancies are the second leading cause of cancer death in the population of developed countries and this trend has become more evident in recent years. The treatment of gastrointestinal malignant tumor mainly adopts surgical radical resection, but the specific range of the surgical resection needs to be determined when the surgical radical resection is specifically carried out, so before the operation, the benign and malignant tumor, the infiltration depth, the metastasis condition, the existence of cancer residue at the cut edge and the like need to be known. Preoperative gastrointestinal endoscopic biopsy is therefore a very important diagnostic evidence for histological diagnosis of gastrointestinal tumors. The operative form of stomach cancer is divided into full stomach resection, secondary stomach resection, partial gastrectomy, endoscopic mucosal or submucosal resection and the like according to the size, the growth position, the infiltration depth and the like of a tumor body.

Currently, gastrointestinal endoscopic biopsy is usually based on gastrointestinal endoscopy, imaging is performed with the aid of CT, MRI, etc., or a number of gastrointestinal diseases are evaluated with a conventional white light laparoscope or endoscope.

However, there are some inevitable disadvantages in performing imaging by imaging using CT, MRI, etc. based on gastrointestinal endoscopy, such as bleeding of intestinal tract or tumor body easily in operation, manual pulling or squeezing, delay of time due to repeated endoscopic biopsy when gastrointestinal endoscopy cannot pass through the intestinal tract, and inconvenience in operation such as additional emergency hemostasis if severe bleeding is caused. Therefore, in view of the current gastrointestinal tract auxiliary detection technology, a new gastrointestinal tract tumor detection device is urgently needed to detect gastrointestinal tract tissue information at different depths in situ in real time and conveniently.

SUMMERY OF THE UTILITY MODEL

To the technical problem that exists among the prior art, the embodiment of the utility model provides a formula cavity endoscope detection device and laser scanning cavity endoscope can zoom.

In a first aspect, an embodiment of the present invention provides a variable focus cavity endoscope detection device, including:

outer fixed casing and interior clamping device, interior clamping device set up in the outer fixed casing, outer fixed casing inboard is provided with the motor that zooms for the drive interior clamping device reciprocates relative to outer fixed casing, be provided with first light path and second light path in the interior clamping device, wherein:

the first light path is used for receiving the pulse laser signal and transmitting and outputting the pulse laser signal to a spontaneous fluorescent substance in a body cell of a body cavity of a to-be-detected living body;

the second optical path is used for collecting and transmitting a fluorescence signal and a second harmonic signal generated by the excitation of the autofluorescent substance.

In a second aspect, an embodiment of the present invention provides an endoscope in a three-dimensional nonlinear laser scanning cavity, including:

fluorescence collection device, scanning acquisition controller, femto second pulse laser instrument, fiber coupling module and the embodiment of the utility model provides a formula cavity speculum detecting device can zoom that the first aspect provided, fluorescence collection device with fiber coupling module all with formula cavity speculum detecting device fiber communication connection can zoom, fluorescence collection device with formula cavity endoscope detecting device can zoom all with scanning acquisition controller electricity is connected, wherein:

the femtosecond pulse laser is used for outputting pulse laser signals to the optical fiber coupling module;

the optical fiber coupling module is used for coupling the pulse laser signal output by the femtosecond pulse laser and transmitting the pulse laser signal to the variable-focus cavity endoscope detection device;

the fluorescence collecting device is used for receiving the fluorescence signal and the second harmonic signal transmitted by the variable-focus cavity endoscope detection device and then respectively converting the fluorescence signal and the second harmonic signal into corresponding electric signals;

and the scanning acquisition controller is used for controlling a scanning galvanometer in the variable-focus cavity endoscope detection device to scan the pulse laser signals and synchronously acquire the electric signals.

The embodiment of the utility model provides a formula of can zooming cavity endoscope detection device and laser scanning cavity endoscope realize the relative reciprocating each other of integrated interior clamping device and external fixation casing through the motor that zooms, thereby make whole light path structure zoom the operation as required in the interior clamping device, realize exploring time measuring to human stomach tissue or oral cavity tissue, can carry out the histiocyte imaging of the different degree of depth, with the structural information who acquires the histiocyte different degree of depth, thereby judge the infiltration degree of depth of tumour more accurately, the condition of shifting and surgery operation reason have the relevant condition such as no cancer remains, and is easy to operate, high durability and convenient use.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic structural view of a variable-focus cavity endoscope detection device according to an embodiment of the present invention;

fig. 2 is a schematic structural view of a variable-focus cavity endoscope detection device according to another embodiment of the present invention;

fig. 3 is a schematic structural view of a variable-focus cavity endoscope detection device according to still another embodiment of the present invention;

fig. 4 is a schematic structural view of a variable-focus cavity endoscope detection device according to another embodiment of the present invention;

fig. 5 is a schematic view of an endoscope structure in a three-dimensional nonlinear laser scanning cavity according to an embodiment of the present invention;

fig. 6 is a schematic view of an endoscope structure in a three-dimensional nonlinear laser scanning cavity according to another embodiment of the present invention;

fig. 7 is a schematic structural view of a fluorescence collecting device according to an embodiment of the present invention;

fig. 8 is a schematic view of a box sealing structure of a box combination structure of a three-dimensional nonlinear laser scanning cavity endoscope according to an embodiment of the present invention;

fig. 9 is a schematic view of a box sealing structure of a box combination structure of a three-dimensional nonlinear laser scanning cavity endoscope according to another embodiment of the present invention;

fig. 10 is a schematic view of a table-type structure of an endoscope in a three-dimensional nonlinear laser scanning cavity according to an embodiment of the present invention;

fig. 11 is a schematic diagram of a table-top configuration of a three-dimensional nonlinear laser scanning cavity endoscope according to another embodiment of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

At present, the gastrointestinal endoscope is taken as a basis, imaging is carried out by taking CT, MRI and the like as assistance to obtain relevant information of malignancy, infiltration depth, metastasis condition, whether cancer residue exists at incisal margin and the like of tumors, and the method has some defects in specific operation, such as easy bleeding of intestinal tracts or tumor bodies, need of manual drawing or extrusion, delay of time due to repeated endoscopic biopsy when the gastrointestinal endoscope cannot pass through the intestinal tracts, and need of additional emergency hemostasis if severe bleeding is caused. In clinical practice, auxiliary examination hands such as CT and MRI cannot accurately judge the infiltration depth of early gastrointestinal tumors and lymph node metastasis. The gastrointestinal tumor T stage is judged by the ultrasonic endoscope, the accuracy reported in the literature is only 44.7% -78%, the judgment is not enough to become a reliable diagnosis standard, the preoperative judgment effect of the ultrasonic endoscope on the local resection operation is poor, the gastrointestinal mucosa levels cannot be accurately subdivided, and the N stage effect is also poor.

While conventional white light laparoscopes and endoscopes can assess many gastrointestinal diseases, the technique is limited to detecting gross morphological changes. Although suspicious regions are easily found, these techniques are associated with false positive rates, specificity, and the like, as compared to in vivo detection techniques. White light endoscopy is associated with wide error in diagnosis of microscopic changes, including examination diagnosis of ulcerative colitis or Barrett's esophagus and flat adenomatous dysplasia. Confocal endoscopy combines laser technology, fluorescence detection technology, rapid scanning technology and the like, and is concerned widely because the confocal endoscope can detect mucosal changes at a microscopic level and can possibly be used for replacing tissue biopsy, and the imaging technology has high sensitivity and specificity. However, the confocal endoscopic imaging technology is still limited by the imaging depth and the fluorescent staining agent, because the gastrointestinal sample has strong absorption and scattering to visible light, the imaging depth is only on the superficial layer, and a specific fluorescent staining developer is required to be injected, the operation is too complicated, and the relevant information such as the infiltration depth of the tumor, the metastasis condition, the existence of cancer residue at the surgical incisal margin and the like cannot be accurately obtained.

The two-photon microscopic imaging technology adopts a femtosecond pulse laser with longer wavelength as an excitation light source, has the characteristics of deep imaging depth, small light damage, small photobleaching area, high fluorescence collection efficiency and the like, and has epoch-making significance in deep imaging of biological tissues. The first two-photon fluorescence microscope in the world was developed by w.denk et al at cornell university in 1990, using a multi-photon microscopic imaging technique based on nonlinear optics and femtosecond pulsed laser. The technology can rapidly obtain the tissue structure and the cell morphology of the specimen in real time by utilizing autofluorescence generated by cells in living tissues and second harmonic generated by collagen tissues. As early as 1986, the second harmonic was used for skin studies and coronary microscopic imaging studies, confirming its feasibility for being used to observe biological tissues. MPM is also an important tool for cancer research. The autofluorescence generated by the cell is derived from Nicotinamide Adenine Dinucleotide (NADH) and Flavin Adenine Dinucleotide (FAD) in the cell, the wavelength emitted by NADH is 460nm, and the second oscillation harmonic of collagen is 370-390 nm, so that a multiphoton microscope with a range of 780-940 nm is usually selected when observing tumor sample tissues. Not only does MPM imaging match standard tumor histopathology, it also provides additional information about the tumor neogenesis process, as can the metabolic levels of tumor tissue cells by measuring the ratio NADH/FAD.

With multiphoton imaging techniques, multiphoton microscopy is capable of providing real-time gastrointestinal tract tissue structure and cell morphology information. The multi-photon imaging technology has the characteristics of no need of exogenous labeled tissues, extremely sensitivity to collagen, small photodamage to tissues, deep penetration depth and the like, and can be possibly applied to optical biopsy of gastrointestinal tumors. There are no clinically available two-photon laparoscopes and endoscopes, and no two-photon imaging-based intracavity scope detection device to detect gastrointestinal tract tissue information in situ and in real time.

For the different degree of depth's of normal position real-time detection intestines and stomach tissue information, the embodiment of the utility model provides a formula cavity endoscope detecting device can zoom, figure 1 is the utility model discloses a formula cavity endoscope detecting device structure sketch map can zoom that an embodiment provided, as shown in figure 1, this formula cavity endoscope detecting device can zoom includes:

outer fixed casing 11 and interior clamping device 12, interior clamping device 12 sets up in outer fixed casing 11, and outer fixed casing 11 inboard is provided with zoom motor 113 for clamping device 12 reciprocates relatively outer fixed casing 11 in the drive, is provided with first light path and second light path in the interior clamping device 12, wherein:

the first light path is used for receiving the pulse laser signal and transmitting and outputting the pulse laser signal to the autofluorescence in the somatic cell of the body cavity of the to-be-detected living body;

the second optical path is used for collecting and transmitting a fluorescent signal and a second harmonic signal generated by the excitation of the autofluorescent substance.

Specifically, the variable-focus cavity endoscope detection device provided by the embodiment of the present invention comprises two main structures, namely an external fixing shell 11 and an internal clamping device 12, wherein the internal clamping device 12 is a whole body, and a light path for transmitting a pulse laser signal and collecting a two-photon signal and a second harmonic signal is arranged in the internal clamping device 12, and the light path is respectively a first light path and a second light path, the first light path receives the pulse laser signal and transmits the output pulse laser signal to an autofluorescence substance in a cavity cell of a living body to be detected, the second light path collects and transmits a fluorescence signal generated by exciting the autofluorescence substance and a second harmonic signal to a fluorescence collecting device in the three-dimensional nonlinear laser scanning cavity endoscope, wherein the first light path and the second light path are both arranged in the internal clamping device 12 to form a whole body, and move up and down relative to the external fixing shell 11 under the driving of the zoom motor 113, so as to realize the tissue cell detection at different depths and acquire the cell structure information at different depths.

The embodiment of the utility model provides a formula of can zooming cavity endoscope detection device realizes the relative reciprocating each other of the interior clamping device and the external fixation casing of integration through the motor that zooms, thereby make whole light path structure zoom the operation as required in the interior clamping device, realize exploring time measuring to human intestines and stomach tissue or oral cavity tissue, can carry out the histiocyte imaging of the different degree of depth, with the structural information who acquires the histiocyte different degree of depth, thereby judge the infiltration degree of depth of tumour more accurately, it has the relevant circumstances such as no cancer remains to shift the condition and surgical operation reason, and is simple in operation, high durability and convenient use.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides a first light path among the formula of can zooming cavity endoscope detecting device is including the first light, second light and the third light that meet in proper order, wherein:

the pulse laser signal transmits the collimating lens, the analyzer, the polarization beam splitter, the first quarter-wave plate and the scanning galvanometer in the first light path to form first light;

after the first light is formed, the pulse laser signal is reflected by the scanning galvanometer and then transmits the first quarter-wave plate to the polarization spectroscope again to form second light;

after the second light is formed, the pulse laser signal is reflected by the polarizing beam splitter and then enters the objective lens through the scanning mirror, the dichroic mirror and the relay lens to form third light;

the second optical path sequentially comprises an objective lens, a relay lens and a dichroic mirror, wherein the objective lens is used for collecting and transmitting a fluorescent signal and a second harmonic signal. As shown in fig. 1, the embodiment of the present invention provides a variable-focus cavity endoscope detection device, wherein a first light path is used for processing a pulse laser signal, the pulse laser signal passes through an optical device of the first light path, and the light path is turned back to form three light beams, which are respectively a first light beam, a second light beam and a third light beam, a collimating lens 121, an analyzer 122, a polarization beam splitter 123, a first quarter-wave plate 124 and a scanning galvanometer 125 are arranged in the light path for forming the first light beam, the scanning galvanometer 125, the first quarter-wave plate 124 and the polarization beam splitter 123 are arranged in the light path for forming the second light beam, and the polarization beam splitter 123, a transmission scanning mirror 126, a dichroic mirror 127, a relay mirror 128 and an objective 129 are arranged in the light path for forming the third light beam;

after the pulse laser signal is emitted from the optical fiber, the pulse laser signal enters the analyzer 122 after being collimated by the collimating lens 121, so that collimated light is changed into linearly polarized light, the polarization direction of the linearly polarized light is consistent with the transmission polarization direction of the polarization beam splitter 123, therefore, the linearly polarized light enters the polarization beam splitter 123, directly transmits through the polarization beam splitter 123, enters the first quarter-wave plate 124 positioned on the left side of the polarization beam splitter 123, the fast axis direction of the first quarter-wave plate forms an angle of +/-45 degrees with the polarization direction of the linearly polarized light, and after passing through the first quarter-wave plate 124, the linearly polarized light is changed into circularly polarized light and enters the scanning polarizer 125 to form the first light; after the first light is formed, the circularly polarized light reflected by the scanning galvanometer 125 enters the first quarter-wave plate 124 again to be changed into linearly polarized light, wherein the polarization direction of the linearly polarized light is perpendicular to the transmission polarization direction of the polarizing beam splitter 123, so that the linearly polarized light is reflected on the splitting surface of the polarizing beam splitter 123 and exits from the lower part of the polarizing beam splitter 123 to form a second light; after the second light is formed, the emergent collimated light enters the scanning mirror to be converged, passes through the relay lens 128 and enters the objective lens 129, and the focal point of the objective lens is located at the relay image surface to form a third light; scanning the mirror 125 in the XY axis sweeps the pulsed laser signal focus over the relay image plane. The pulse laser signal is transmitted through a parallel plate as a dichroic mirror 127, and the dichroic mirror 127 distinguishes the pulse laser signal and a fluorescent signal and a second harmonic signal generated by excitation of an autofluorescent substance according to the wavelength, transmits the pulse laser signal therethrough, and reflects the fluorescent signal and the second harmonic signal. The pulse laser signal is converged by the scanning mirror 126 to generate a relay image surface, and is transmitted by the relay mirror 128 to coincide with the rear image surface of the objective lens 129. The relay image produced by the pulsed laser signal scan is therefore scaled to the sample according to the magnification of the objective lens 129. The focus of the pulse laser signal on the sample can generate a fluorescence signal and a second harmonic signal, the generated fluorescence signal and the second harmonic signal are collected by the objective 129 and reflected by the dichroic mirror 127 to enter the collection optical fiber bundle for collection, wherein the objective 129 is a finite-distance micro objective 129, and the second optical path sequentially comprises the objective 129 for collecting and transmitting the fluorescence signal and the second harmonic signal, the relay 128 and the dichroic mirror 127; wherein the scanning galvanometer may be based on mechanical, electromechanical or microelectromechanical principles, as is the case with the various embodiments below.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides a first light path among the formula of can zooming cavity endoscope detecting device is including meeting first light, second light, third light, fourth light and fifth light in proper order, wherein:

the pulse laser signal is incident to the polarization spectroscope through a collimating lens and an analyzer in a first light path to form first light;

after the first light is formed, the pulse laser signal is reflected by the polarization beam splitter and then transmits the second quarter wave plate to the plane reflector to form second light;

after the second light is formed, the pulse laser signal is reflected by the plane mirror and then transmits the second quarter wave plate, the polarization beam splitter and the third quarter wave plate to the scanning galvanometer again to form third light;

after the third light is formed, the pulse laser signal is reflected by the scanning galvanometer and then transmits the third quarter-wave plate to the polarization spectroscope again to form a fourth light;

after the fourth light is formed, the pulse laser signal is reflected by the polarizing beam splitter and then enters the objective lens through the scanning mirror, the dichroic mirror and the relay lens to form fifth light;

the second optical path sequentially comprises an objective lens, a relay lens and a dichroic mirror, wherein the objective lens is used for collecting and transmitting a fluorescent signal and a second harmonic signal. That is, fig. 2 is a schematic structural diagram of a variable-focus cavity endoscope detection device according to another embodiment of the present invention, as shown in fig. 2, when a pulse laser signal is processed by a first optical path in the variable-focus cavity endoscope detection device according to an embodiment of the present invention, five light beams are formed by the return of the optical path when the pulse laser signal passes through an optical device of the first optical path, which respectively include a first light beam, a second light beam, a third light beam, a fourth light beam and a fifth light beam that are sequentially connected, a collimating lens 121, an analyzer 122 and a polarization beam splitter 123 are disposed in the optical path forming the first light beam, a polarization beam splitter 123, a second quarter wave plate 124 and a plane reflector 125 are disposed in the optical path forming the second light beam, a plane reflector 125, a second quarter wave plate 124, a polarization beam splitter 123, a third quarter wave plate 126 and a scanning beam splitter 127 are disposed in the optical path forming the third light beam, a scanning galvanometer 127, a third quarter-wave plate 126 and a polarization beam splitter 123 are arranged in the light path for forming the fourth light ray, and a polarization beam splitter 123, a scanning mirror 128, a dichroic mirror 129, a relay lens 130 and an objective lens 131 are arranged in the light path for forming the fifth light ray;

after the pulse laser signal exits from the optical fiber, the pulse laser signal enters the analyzer 122 after being collimated by the collimating lens 121, so that the collimated light is changed into linearly polarized light, the polarization direction of the linearly polarized light is perpendicular to the transmission polarization direction of the polarization beam splitter 123, and therefore the linearly polarized light enters the polarization beam splitter 123, is reflected and exits from the right side of the polarization beam splitter 123, and forms a first light ray; after the first light is formed, the emitted linearly polarized light enters the second quarter-wave plate 124, wherein the fast axis direction of the second quarter-wave plate forms an angle of ± 45 degrees with the linearly polarized light polarization direction, so that the linearly polarized light is changed into circularly polarized light after passing through the second quarter-wave plate and is incident to the plane mirror 125 to form a second light; after the second light is formed, the circularly polarized light is reflected by the plane mirror 125, returns along the original optical path, and is changed into linearly polarized light by the second quarter-wave plate 124 again, and the polarization direction of the linearly polarized light is consistent with the transmission polarization direction of the polarization beam splitter 123, so that the linearly polarized light enters the polarization beam splitter 123 for the second time, directly transmits through the polarization beam splitter 123, enters the third quarter-wave plate 126 positioned at the left side of the polarization beam splitter 123, wherein the fast axis direction of the third quarter-wave plate forms an angle of ± 45 degrees with the polarization direction of the linearly polarized light, and the linearly polarized light is changed into circularly polarized light after passing through the third quarter-wave plate and then enters the scanning polarizer 127 to form; after the fifth light is formed, the circularly polarized light reflected by the scanning galvanometer 127 enters the third quarter-wave plate 126 again to be changed into linearly polarized light, wherein the polarization direction of the linearly polarized light is perpendicular to the transmission polarization direction of the polarization beam splitter 123, so that the polarized light is reflected on the splitting surface of the polarization beam splitter 123 and exits from the lower part of the polarization beam splitter 123 to form a fourth light; after the fourth light is formed, the emergent collimated light enters the scanning mirror to be converged, passes through the relay lens 130 and enters the objective lens 131, and the focal point of the objective lens is located at the relay image surface to form a third light; scanning the scanning galvanometer 127 on the XY axis causes the pulsed laser signal focus to scan across the relay image plane. The pulse laser signal is transmitted through a parallel plate as a dichroic mirror 129, and the dichroic mirror 129 distinguishes the pulse laser signal and a fluorescent signal and a second harmonic signal generated by excitation of an autofluorescent substance according to the wavelength, transmits the pulse laser signal therethrough, and reflects the fluorescent signal and the second harmonic signal. The pulse laser signal is converged by the scanning mirror 128 to generate a relay image surface, and is transmitted by the relay mirror 130 to coincide with the rear image surface of the objective lens 131. The relay image generated by the pulsed laser signal scanning is thus scaled onto the sample according to the magnification of the objective lens 131. The focus of the pulse laser signal on the sample generates a fluorescence signal and a second harmonic signal, the generated fluorescence signal and the second harmonic signal are collected by an objective lens 131 and reflected by a dichroic mirror 129 into a collection fiber bundle, wherein the objective lens 131 is a finite distance micro objective lens 131, and the second optical path sequentially comprises the objective lens 131, a relay lens 130 and the dichroic mirror 129, which collect and transmit the fluorescence signal and the second harmonic signal.

On the basis of each embodiment, the embodiment of the utility model provides an external fixation casing among the formula of zooming cavity endoscope detecting device includes handle casing and detecting tube, as shown in fig. 1, handle casing 111 and detecting tube 112 fixed connection, and the motor 113 that zooms sets up in handle casing 111 inboard, is provided with the detection passageway in the detecting tube 112, wherein:

the collimating lens 121, the analyzer 122, the polarizing beam splitter 123, the first quarter wave plate 124, the scanning galvanometer 125, the scanning mirror 126 and the dichroic mirror 127 in the first optical path are all located in the handle housing 111, the relay lens and the objective lens in the first optical path are both located in the detection channel, and the objective lens is located at the channel port of the detection channel. That is to say, the embodiment of the utility model provides an external fixation casing among the variable-focus cavity endoscope detection device includes handle casing 111 and detecting tube 112 two parts, handle casing 111 and detecting tube 112 fixed connection, and handle casing 111 all has built-in space and two built-in spaces intercommunication each other with detecting tube 112, interior clamping device is located the built-in space of handle casing 111 and detecting tube 112 intercommunication, wherein, collimating lens 121 in the first light path, analyzer 122, polarization beam splitter, first quarter wave plate, scanning galvanometer, scanning mirror and dichroscope all are located handle casing 111, relay 128 and objective 127 in the first light path all are located the detection passageway, objective is located the passageway department of detection passageway, with through objective outwards output laser signal and gather two photon signal and second harmonic signal.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides an external fixation casing among the formula of can zooming cavity endoscope detecting device includes handle casing and detecting tube, as shown in fig. 2, handle casing and detecting tube fixed connection, the motor that zooms sets up in handle casing inboard, is provided with the detection passageway in the detecting tube, wherein:

the collimating lens 121, the analyzer 122, the polarizing beam splitter 123, the second quarter wave plate 124, the plane mirror 125, the third quarter wave plate 126, the scanning galvanometer 127, the scanning mirror 128 and the dichroic mirror 129 in the first optical path are all located in the handle shell, the relay mirror and the objective lens in the first optical path are all located in the detection channel, and the objective lens is located at the channel opening of the detection channel. That is to say the embodiment of the utility model provides an external fixation casing among the variable-focus formula cavity endoscope detection device includes handle casing and detecting tube two parts, handle casing and detecting tube fixed connection, and handle casing and detecting tube all have built-in space and two built-in spaces communicate each other, interior clamping device is located the built-in space of handle casing and detecting tube intercommunication, wherein, collimating lens in the first light path, the analyzer, the polarization spectroscope, second quarter wave plate, the plane mirror, the third quarter wave plate, the scanning galvanometer, scanning mirror and dichroic mirror all are located the handle casing, relay lens 130 and objective 131 in the first light path all are located the detection passageway, objective is located the passageway department of detection passageway, with through objective external output laser signal and gather two photon signal and secondary harmonic signal.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides a variable focal length formula cavity endoscope detection device still includes the adjustable curvature lens of electricity, and fig. 3 is the utility model discloses a variable focal length formula cavity endoscope detection device schematic structure diagram that still another embodiment provided, as shown in fig. 3, the adjustable curvature lens of electricity 120 is located between collimating lens 121 and the analyzer 122, and pulse laser signal transmits collimating lens 121, the adjustable curvature lens of electricity 120, analyzer 122, polarization spectroscope 123, first quarter wave plate 124 to scanning galvanometer 125, forms new first light. That is, the optical elements in the new first light ray sequentially include a collimating lens 121, an electrically adjustable curvature lens 120, an analyzer 122, a polarization beam splitter 123, a first quarter-wave plate 124 and a scanning galvanometer 125. The electrically adjustable curvature lens 120 is disposed such that a corresponding curvature of the surface of the electrically adjustable curvature lens 120 can be generated by applying a voltage or a current to the electrically adjustable curvature lens 120, and thus parallel light emitted from the straight lens 121 can be aligned to generate different focal powers. The specific light path is as follows: laser signals are emitted from the optical fibers, parallelly enter the electric adjustable curvature lens 120 after passing through the collimating lens 121, corresponding focal power is generated from the electric adjustable curvature lens 120 according to loaded voltage or current signals, and emitted convergent or divergent light passes through an optical element in a first light path to form new first light, second light and third light which are transmitted to the objective lens 129 and then converged on a sample. The focal power change introduced by the electrically adjustable curvature lens 120 can make the focal point of the laser signal emitted from the opening of the objective lens 129 move up and down in the depth direction, and the electrically adjustable curvature lens 120 has a very fast response speed, and the scanning frequency is in the order of KHz, so that fast depth direction scanning imaging can be realized. The electrically adjustable curvature lens 120 is equivalent to parallel plate glass when no voltage or current signal is applied, and has no focal power to the laser signal and does not cause any offset of the focus behind the objective lens 129, thereby realizing three-dimensional imaging. When the variable-focus type cavity endoscope detection device is used specifically, the position of the objective 129 is adjusted through the zoom motor, the system is switched to a zoom scanning mode of the electrically adjustable curvature lens 120 after the position is roughly adjusted to the corresponding longitudinal depth position, and the sample is subjected to rapid three-dimensional imaging, wherein when the variable-focus type cavity endoscope detection device is not provided with the zoom motor, the zoom adjustment can be performed only through the electrically adjustable curvature lens 120.

On the basis of each embodiment, the embodiment of the utility model provides a variable-focus cavity endoscope detection device still includes the electrically adjustable curvature lens, as shown in fig. 2, electrically adjustable curvature lens 120 is located between collimating lens 121 and analyzer 122, and pulse laser signal transmits collimating lens 121, electrically adjustable curvature lens 120, analyzer 122 and incides polarization spectroscope 123, forms new first light. I.e. the optical elements in the new first light ray comprise in sequence a collimating lens 121, an electrically adjustable curvature lens 120, an analyzer 122 and a polarizing beam splitter 123. The electrically adjustable curvature lens 120 is configured such that a voltage or a current is applied to the electrically adjustable curvature lens 120 to correspondingly bend the surface of the electrically adjustable curvature lens 120, thereby generating different powers for the parallel light emitted from the collimating lens. The specific light path is as follows: laser signals are emitted from the optical fibers, parallelly enter the electric adjustable curvature lens 120 after passing through the collimating lens, corresponding focal power is generated from the electric adjustable curvature lens 120 according to loaded voltage or current signals, and the emitted convergent or divergent light passes through the optical element in the first light path to form new first light, second light, third light, fourth light and fifth light, and then is transmitted to the objective lens 131 and converged on a sample. The focal power change introduced by the electrically adjustable curvature lens 120 can make the focal point of the laser signal emitted from the opening of the objective lens 131 move up and down in the depth direction, and the electrically adjustable curvature lens 120 has a very fast response speed, and the scanning frequency is in the order of KHz, so that fast depth direction scanning imaging can be realized. The electrically adjustable curvature lens 120 is equivalent to parallel plate glass when no voltage or current signal is applied, and has no focal power to the laser signal and does not cause any offset of the focal point behind the objective lens 131, thereby realizing three-dimensional imaging. When the variable-focus type cavity endoscope detection device is used specifically, the electric adjustable curvature lens 120 is complementary with a zoom motor, the position of the objective lens 131 is adjusted through the zoom motor, after the position is roughly adjusted to the corresponding depth position, the system is switched to a zoom scanning mode of the electric adjustable curvature lens 120, and a sample is subjected to rapid three-dimensional imaging, wherein when the variable-focus type cavity endoscope detection device is not provided with the zoom motor, the zoom adjustment can be performed only through the electric adjustable curvature lens 120.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides a still be provided with a plurality of illumination passageways in the detection pipe among the formula of can zooming cavity endoscope detection device, be provided with the illumination fiber bundle that is used for transmitting illumination light signal in the illumination passageway, wherein the illumination passageway uses the axle center of detecting the passageway as center evenly distributed. Fig. 4 is promptly the utility model discloses the formula of can zooming endoscope detection device structure sketch map that still another embodiment provided, as shown in fig. 4, the utility model provides a still be provided with a plurality of illumination channels 1122 in the detection pipe among the formula of can zooming endoscope detection device, this illumination channel is more than one, all is provided with the illumination fiber bundle in every passageway, illumination fiber has certain aperture angle, does not need lens can directly be used for dispersing the illumination, and the illumination channel uses the axle center of detection channel as central evenly distributed, provides even illumination for the formula of can zooming endoscope detection device to tissue region state that awaits measuring before the convenient work observation objective.

On the basis of each embodiment, the embodiment of the utility model provides a can zoom in among the cavity endoscope detecting device in the detecting tube still be provided with the observation passageway, as shown in fig. 4, observe the passageway and be located between detection passageway and the illumination passageway, wherein:

an observation lens is arranged at the channel opening of the observation channel and is connected with the bright field optical fiber bundle in the observation channel so as to acquire image information of the tissue area to be detected in front of the objective lens. Namely the embodiment of the utility model provides a can zoom still be provided with in the detecting tube among the cavity endoscope detecting device and observe the passageway, should observe the passageway and be located between detecting channel and the illumination passageway, and be provided with observation lens 1121 and bright field fiber bundle, and bright field fiber bundle is imaging fiber bundle promptly for the regional image information of the tissue that awaits measuring before the objective that transmission observation lens 1121 caught, wherein observe the passageway can be one, also can be for two formation binocular observation, realize three-dimensional bright field cavity internal sight glass function.

On the basis of each embodiment, the embodiment of the utility model provides a can zoom formula cavity endoscope detecting device in still be provided with in the detecting tube and adsorb the passageway, as shown in fig. 4, adsorb passageway 1123 and be located between illumination passageway and the detecting tube edge. Namely the embodiment of the utility model provides a still be provided with in the detecting tube among the formula of zooming cavity endoscope detecting device and be used for making the formula of zooming cavity endoscope detecting device adsorb the absorption passageway on the tissue that awaits measuring, adsorb the air in the passageway through the extraction, form the negative pressure in adsorbing passageway 1123 for the formula of zooming cavity endoscope detecting device adsorbs on the tissue that awaits measuring, wherein, it is located between illumination passageway and the detecting tube edge to adsorb the passageway, is located the illumination passageway outside promptly, is close to the position department of detecting tube avris.

On the basis of the above embodiments, the embodiment of the utility model provides a button hole has been seted up on the handle casing among the variable focus cavity endoscope detecting device, is provided with switching button and imaging button in the button hole, and switching button is used for switching different light filters to acquire the illumination light signal of different wavelengths;

the imaging button is used for controlling an imaging module connected with the bright field optical fiber bundle to image a tissue area to be detected in front of the objective lens. Namely, the switching button is arranged in the button hole of the handle shell in the variable-focus cavity endoscope detection device provided by the embodiment of the utility model, and the optical filters for filtering the illumination light signals with different wavelengths can be switched through the switching button, so that the workers can select the illumination light signals with different wavelengths transmitted; an imaging button is arranged in a button hole of a handle shell in the variable-focus cavity endoscope detection device, and an imaging module connected with the bright field optical fiber bundle can be controlled by the imaging button to photograph and image a tissue area to be detected in front of an objective lens.

The embodiment of the utility model provides a still provide a three-dimensional nonlinear laser scanning cavity inside speculum, fig. 5 does the utility model relates to a three-dimensional nonlinear laser scanning cavity inside speculum structure sketch map that the embodiment provided, as shown in fig. 5, this three-dimensional nonlinear laser scanning cavity inside speculum includes:

fluorescence collection device 56, scanning acquisition controller 531, femto second pulse laser, fiber coupling module and the variable focal length cavity endoscope detecting device 1 that each embodiment provided above, fluorescence collection device 56 and fiber coupling module all with variable focal length cavity endoscope detecting device 1 optical fiber communication connection, fluorescence collection device and variable focal length cavity endoscope detecting device all with scanning acquisition controller 531 electricity be connected, wherein:

the optical fiber coupling module is used for coupling a pulse laser signal output by the femtosecond pulse laser and transmitting the pulse laser signal to the variable-focus cavity endoscope detection device 1;

the fluorescence collecting device 56 is used for receiving the fluorescence signal and the second harmonic signal transmitted by the variable-focus cavity endoscope detection device and respectively converting the fluorescence signal and the second harmonic signal into corresponding electric signals;

and the scanning acquisition controller 531 is used for controlling a scanning galvanometer in the variable-focus cavity endoscope detection device 1 to scan the pulse laser signals and synchronously acquire electric signals.

Specifically, the embodiment of the present invention provides a three-dimensional nonlinear laser scanning cavity endoscope, which comprises a fluorescence collecting device 56, a scanning collecting controller 531, a femtosecond pulse laser, a fiber coupling module and a variable-focus cavity endoscope detecting device 1, thereby forming a three-dimensional nonlinear laser scanning cavity endoscope for detecting gastrointestinal tissues and oral tissues by using a two-photon imaging technique, wherein the femtosecond pulse laser can emit pulse laser signals for exciting the spontaneous fluorescence in the gastrointestinal tissues and oral tissue cells of a human body, so as to generate a multiphoton fluorescence signal and a second harmonic signal, including using the 920nm femtosecond pulse laser to excite FAD and collagen in cells, exciting the 500-600nm fluorescence signal and the 460nm second harmonic signal, and exciting the spontaneous fluorescence such as FAD or NADH in cells by the 780nm femtosecond pulse laser, to generate corresponding fluorescence signal and second harmonic signal, wherein the femtosecond pulse laser and the fiber coupling module are combined together to form a laser emission module 540;

the fluorescence collecting device 56 integrates two signal collecting optical paths, namely a fluorescence signal collecting optical path and a second harmonic signal collecting optical path, to respectively collect the fluorescence signal and the second harmonic signal; the scanning acquisition controller 531 controls the scanning galvanometer to scan the pulse laser signal and excite the autofluorescence substance to generate a fluorescence signal and a second harmonic signal, and acquires a first electrical signal and a second electrical signal obtained by converting the fluorescence signal and the second harmonic signal by the fluorescence collection device; the three-dimensional nonlinear laser scanning cavity endoscope can be divided into a laparoscope and a stomatoscope according to different structures of the variable-focus cavity endoscope detection device 1. The resolution of the three-dimensional nonlinear laser scanning cavity endoscope can be set to 800nm, the imaging field of view can be 400 micrometers by 400 micrometers, and the imaging speed can be 26 frames (256 pixels by 256) or 13 frames (512 pixels by 512 pixels).

The embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope adopts fluorescence collection device, scanning acquisition controller, femto second pulse laser, optical fiber coupling module and variable focal length cavity endoscope detection device, thereby form the laser scanning cavity endoscope that utilizes two-photon imaging technique to survey human intestines and stomach tissue and oral cavity tissue, adjust objective focal length through liquid lens and zoom motor, realize that laser scanning microscope carries out three-dimensional scanning to the cell structure of different degree of depth, excite the intracellular fluorescent substance spontaneous acquisition multiphoton fluorescence signal and second harmonic signal through femto second pulse laser, realize laser scanning microscope nonlinearity, collect fluorescence signal and second harmonic signal through fluorescence collection device, and convert corresponding electric signal into, and then obtain corresponding fluorescence image etc. that reflects cell tissue structure through this electric signal, wherein, the adoption of the variable-focus cavity endoscope detection device can make the staff can be nimble to survey the gastrointestinal tissue, the oral tissue and the tissue in the uterine cavity in the abdominal cavity of the human body, when surveying the gastrointestinal tissue of the human body, only need to set up a small opening on the abdomen of the human body, so reduce the operation cost and the pain of the patient, when imaging the tissue in the uterine cavity, the noninvasive detection is carried out through the natural channel (vagina), the equipment operation is simple, and the use is convenient.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope still includes lighting module and imaging module, as shown in fig. 5, lighting module 534 and imaging module 533 all with the cavity endoscope detecting device optical fiber communication connection of zooming, wherein:

the illumination module 534 sequentially comprises an illumination lens 5342, a variable filter 5341 and an illumination light source 5343, wherein the illumination lens 5342 is connected with the illumination optical fiber bundle, and the illumination light source is used for providing an illumination light signal;

the imaging module 533 sequentially includes an imaging lens 5331 and a camera 5332, the imaging lens 5331 is connected to the bright field optical fiber bundle, and the camera 5332 is configured to acquire image information of a tissue region to be measured. That is to say, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope still is provided with illumination module 534 and imaging module 533, wherein, illumination module 534 includes illuminating lens 5342, variable optical filter 5341 and illuminating light source 5343 in proper order, wherein, illuminating light source can switch different optical filters through electronic variable optical filter runner, in order to obtain the illuminating light signal of different wavelengths, the fundamental principle is that two-photon fluorescence imaging is not disturbed, for example when obtaining autofluorescence and second harmonic, can switch to red or infrared optical filter 635, in order to obtain 370nm, illuminating light nm or infrared 850nm, the illuminating light signal of 940nm, illuminating light signal gets into illumination fiber bundle through the lens coupling;

the imaging module 533 sequentially includes an imaging lens and a camera, the lens being focused on the camera for direct observation of bright field. The two cameras correspond to binocular bright field optical fiber bundles, bright field imaging and two-photon imaging form a multi-mode laparoscope, and bright field binocular three-dimensional laparoscope is in a mode, so that large-view sample observation is carried out, and the basic appearance of a sample is mainly observed. For the suspicious or interested area, the method can be switched to a two-photon mode to perform autofluorescence and second harmonic imaging, and observe the cell grade morphology of the sample, thereby providing a basis for further judgment.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope, still include air exhaust device, as shown in fig. 5, air exhaust device 52 mainly includes the aspiration pump, link to each other with the absorption passageway through the exhaust pipe, set up the bleeder valve in the exhaust pipe, the bleeder valve is connected with air exhaust device 52 electricity, air exhaust device 52 is through the size of the switch and the switching of adjustment bleeder valve, the bleed-off flow of control exhaust pipe, thereby realize the control of bleeding to the absorption passageway, and then adjust the negative pressure in the absorption passageway, make variable-focus formula cavity endoscope detection device pass through the effect of atmospheric pressure, adsorb on tissues such as human abdominal cavity intestines and stomach, oral cavity and palace chamber, reduce the motion artifact that the organism tissue activity brought, make formation of image more stable, it is clear.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope still includes the industrial computer, as shown in fig. 5, industrial computer 532 is connected with scanning acquisition controller 531 electricity, wherein:

the industrial personal computer 532 is configured to obtain the first electrical signal and the second electrical signal acquired by the scanning acquisition controller 531, generate a first fluorescent image based on the first electrical signal, and generate a second fluorescent image based on the second electrical signal. Namely the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope still includes the industrial computer 532 with scan acquisition controller 531 electricity is connected, this industrial computer 532 is based on first signal of telecommunication generation first fluorescence image and based on the second signal of telecommunication generation second fluorescence image, can be used for showing cell structure and fibrous structure information respectively, wherein installs control software on the industrial computer, through control software, sends control command to the scanner to control scan acquisition controller, acquire above-mentioned first signal of telecommunication and second signal of telecommunication.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope still includes the display, as shown in fig. 5, display 55 is connected with industrial computer 532 electricity for show first fluorescence image and second fluorescence image. Namely, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope is still including being used for showing display 55 of first fluorescence image and second fluorescence image, through display 55, the staff can directly acquire the relevant information of first fluorescence image and second fluorescence image.

Wherein, fig. 6 is a schematic view of the endoscope structure in the three-dimensional nonlinear laser scanning cavity provided by another embodiment of the present invention, as shown in fig. 6, the endoscope structure in the three-dimensional nonlinear laser scanning cavity also includes:

the fluorescence collection device 56, the scanning collection controller 531, the femtosecond pulse laser, the optical fiber coupling module, the variable-focus cavity endoscope detection device 1, the air extractor 52, the industrial personal computer 532, the illumination module 534 and the imaging module 533 provided by the above embodiments, wherein the fluorescence collection device 56 and the optical fiber coupling module are both in optical fiber communication connection with the variable-focus cavity endoscope detection device 1, the fluorescence collection device 56 and the variable-focus cavity endoscope detection device 1 are both electrically connected with the scanning collection controller 531, wherein the functions of the above modules or devices are the same as the functions of the devices in the above embodiments, the modules or devices comprise the femtosecond pulse laser and the optical fiber coupling module which are combined together to form a laser emission module 540, the illumination module 534 sequentially comprises an illumination lens 5342, a variable optical filter 5341 and an illumination light source 5343, and the imaging module 533 sequentially comprises an imaging lens and a camera, the variable-focus cavity endoscope detection device 1 in the three-dimensional nonlinear laser scanning cavity endoscope is a stomatoscope detection device, and a light path structure of the variable-focus cavity endoscope detection device comprises a liquid lens, and the function of the liquid lens is the same as that of the liquid lens in the embodiments.

On the basis of the foregoing embodiments, fig. 7 is a structural schematic diagram of the fluorescence collecting device provided by the embodiment of the present invention, as shown in fig. 7, the embodiment of the present invention provides a fluorescence collecting device including collecting the general optical fiber interface 881, the first photomultiplier 882, the second photomultiplier 883, and the first collecting optical path between the collecting general optical fiber interface 881 and the first photomultiplier 882, and the second collecting optical path between the collecting general optical fiber interface 881 and the second photomultiplier 883, wherein:

the first collecting light path sequentially comprises a coupling lens 81, an infrared filter 82, a first dichroic mirror 83, a first filter 84 and a first collecting lens 85, wherein the first collecting light path is used for collecting the fluorescent signal received by the fluorescent collecting device, and the first photomultiplier 882 is used for converting the fluorescent signal into a first electrical signal;

the second collecting light path sequentially includes a coupling lens 81, an infrared filter 82, a first dichroic mirror 83, a second dichroic mirror 86, a second filter 87, and a second collecting lens 88, wherein the second collecting light path is configured to collect a second harmonic signal received by the fluorescence collecting device, and the second photomultiplier 883 is configured to convert the second harmonic signal into a second electrical signal. That is, the fluorescence collecting device provided by the embodiment of the present invention has a two-way signal collecting function, and integrates two light paths, wherein the first dichroic mirror 83 in the first collecting light path is a transmission fluorescence signal, the dichroic mirror for reflecting the second harmonic, the second dichroic mirror 86 and the first dichroic mirror 83 are the same dichroic mirror, and are used for reflecting the second harmonic, the first optical filter 84 is used for transmitting the fluorescence signal, and filtering the rest interference signals, and the second optical filter 87 is used for transmitting the corresponding second harmonic signal, and filtering the rest interference signals, for example, when using 780nm femtosecond fiber laser to excite the autofluorescence substance in the human abdominal cavity or oral cavity cell, 390nm second harmonic signal and 450 and 600nm two-photon autofluorescence signal can be obtained, the wavelength passes through above 420nm, the dichroic mirror with wavelength reflection below 420, that is, the first dichroic mirror 83, can separate two paths of fluorescence, clean second harmonic signals and fluorescence signals can be obtained by using the first filter 84 of 390 + -20 nm and the second filter 87 of 450-600nm, respectively.

Wherein, fig. 8 is the utility model provides a box-sealing structure schematic diagram of box integrated configuration of three-dimensional nonlinear laser scanning cavity endoscope, as shown in fig. 8, display 55 is integrated together with the box integration of installing each module on the case lid, make things convenient for whole equipment to remove, and change the workplace, and this display 55 when using, can place outward on the box, in order to make things convenient for the staff to acquire the information on the display, wherein the variable focus formula cavity endoscope in this three-dimensional nonlinear laser scanning cavity endoscope is interior speculum detecting device 1 is stomatoscope detecting device. After the three-dimensional nonlinear laser scanning cavity endoscope is used, a worker can carry the equipment box by hand, so that the working place can be conveniently replaced, and the equipment can be more conveniently used particularly in hospitals, laboratories or outdoor places.

Wherein, fig. 9 is the utility model discloses another embodiment provides a box integrated configuration's of three-dimensional nonlinear laser scanning cavity endoscope envelope structure sketch map, as shown in fig. 9, display 55 is integrated together with the box integration of installing each module on the case lid, make things convenient for whole equipment to remove, and change the workplace, and this display 55 when using, can place outward on the box, in order to make things convenient for the staff to acquire the information on the display, wherein the variable focus formula cavity endoscope in this three-dimensional nonlinear laser scanning cavity endoscope is endoscope detection device 1, and laparoscope detection device can set up a plurality ofly simultaneously. After the three-dimensional nonlinear laser scanning cavity endoscope is used, a worker can carry the equipment box by hand, so that the working place can be conveniently replaced, and the equipment can be more conveniently used particularly in hospitals, laboratories or outdoor places.

On the basis of above-mentioned each embodiment, the utility model provides a variable focal length formula cavity endoscope detection device among three-dimensional nonlinear laser scanning cavity endoscope is a plurality of. Namely the embodiment of the utility model provides a fluorescence collection device and fiber coupling module can be simultaneously with a plurality of formula cavity endoscope detection device optical fiber communication connection of zooming, a plurality of detection device of integration in a three-dimensional nonlinear laser scanning cavity endoscope system promptly to the realization is surveyed the different positions of intestines and stomach tissue simultaneously, thereby carries out contrastive analysis.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope still is including adjusting optic fibre for fluorescence collection device and fiber coupling module respectively with the variable focus type cavity endoscope detection device between the optical fiber transmission be connected, wherein:

the length of the adjusting optical fiber can be adjusted. Namely the embodiment of the utility model provides a fluorescence collection device and fiber coupling module in three-dimensional nonlinear laser scanning cavity endoscope carry out optical fiber transmission through adjustable length's regulation optic fibre and variable focal cavity endoscope detecting device respectively and are connected, in order to realize according to different experiment scene needs, carry out nimble detection device that removes, avoid limited fiber length's restriction, wherein, the adjustable length of adjusting optic fibre, for the optic fibre through changing different length, realize the application of various occasions, can carry out the optic fibre change of different length as required at any time.

To the three-dimensional nonlinear laser scanning cavity endoscope provided by the above embodiments, the embodiment of the present invention provides another specific implementation manner, fig. 10 is a schematic diagram of a table structure of the three-dimensional nonlinear laser scanning cavity endoscope provided by an embodiment of the present invention, as shown in fig. 10, the three-dimensional nonlinear laser scanning cavity endoscope includes an air extractor 52, a first device 53, a second device 54, a display 55 and a variable-focus cavity endoscope detection device 1, wherein the first device 53 integrates a scanning acquisition controller and an industrial personal computer, the industrial personal computer is electrically connected to the display 55, the second device 54 integrates a femtosecond pulse laser, an optical fiber coupling module and a fluorescence collection device, an illumination module and an imaging module, the optical fiber coupling module and the fluorescence collection device are all connected to the absorption microscope detection device 51 by optical fiber transmission, the variable-focus cavity endoscope detection device 1 is a mouth cavity endoscope detection device, and is used for detecting oral tissues of a human body so as to know information such as benign and malignant tumors, infiltration depth, metastasis conditions, and whether cancer residues exist at incisional edges.

Wherein, fig. 11 shows another embodiment of the present invention provides a table structure diagram of a three-dimensional nonlinear laser scanning cavity endoscope, as shown in fig. 11, the three-dimensional nonlinear laser scanning cavity endoscope also comprises an air extractor 52, a first device 53, a second device 54, a display 55 and a variable-focus cavity endoscope detection device 1, wherein the first device 53 integrates a scanning acquisition controller and an industrial control computer, the industrial control computer is electrically connected with the display 55, the second device 54 integrates a femtosecond pulse laser, an optical fiber coupling module and a fluorescence collection device, an illumination module and an imaging module, the optical fiber coupling module and the fluorescence collection device are both in optical fiber transmission connection with an adsorption detection microscope device 51, wherein the variable-focus cavity endoscope detection device 1 is a laparoscope detection device embedded in the abdomen of a human body for detecting gastrointestinal tissues, in addition, the laparoscope based on the laparoscope detection device can also be used for detecting the tissues in the uterine cavity of a woman, and the imaging principle of the detected tissues is the same as that of the cavity endoscope of each embodiment.

Although the present invention has been described with reference to the accompanying drawings, it is not intended to limit the scope of the invention, and it should be understood by those skilled in the art that the above is not required to be limited to the preferred embodiments of the present invention, but rather, the present invention is not limited to the above embodiments. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

The above-described embodiments of the apparatus are merely illustrative, and the units described as separate parts may or may not be physically separate, and the parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of the present embodiment. One of ordinary skill in the art can understand and implement it without inventive effort.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.

Claims

1. A variable focus cavity endoscope probe, comprising:

2. The variable focus endoscope detection device according to claim 1, wherein said first optical path comprises a first light ray, a second light ray and a third light ray connected in sequence, wherein:

after the second light is formed, the pulse laser signal is reflected by the polarizing beam splitter and then enters an objective lens through a scanning mirror, a dichroic mirror and a relay lens to form third light;

the second optical path sequentially comprises the objective lens, the relay lens and the dichroic mirror which collect and conduct the fluorescent signal and the second harmonic signal.

3. The variable focus endoscope detection device according to claim 1, wherein said first optical path comprises a first light ray, a second light ray, a third light ray, a fourth light ray and a fifth light ray connected in sequence, wherein:

the pulse laser signal is incident to a polarization spectroscope through a collimating lens and an analyzer in the first light path to form first light;

after the first light is formed, the pulse laser signal is reflected by the polarization beam splitter and then transmits a second quarter wave plate to the plane reflector to form a second light;

after the second light is formed, the pulse laser signal is reflected by the plane mirror and then transmits the second quarter wave plate, the polarization beam splitter and the third quarter wave plate to the scanning galvanometer again to form a third light;

after the third light is formed, the pulse laser signal is reflected by the scanning galvanometer and then transmits the third quarter wave plate to the polarization spectroscope again to form fourth light;

after the fourth light is formed, the pulse laser signal is reflected by the polarizing beam splitter and then enters an objective lens through a scanning mirror, a dichroic mirror and a relay lens to form fifth light;

4. The variable focus cavity endoscope apparatus of claim 2, wherein the outer stationary housing comprises a handle housing and a probe tube, the handle housing is fixedly connected to the probe tube, the zoom motor is disposed inside the handle housing, the probe tube has a probe channel therein, wherein:

the collimating lens, the analyzer, the polarizing beam splitter, the first quarter wave plate, the scanning galvanometer, the scanning mirror and the dichroic mirror in the first light path are all located in the handle shell, the relay mirror and the objective lens in the first light path are all located in the detection channel, and the objective lens is located at a channel port of the detection channel.

5. The variable focus endoscope probing apparatus according to claim 3, wherein said outer stationary housing comprises a handle housing and a probe tube, said handle housing is fixedly connected to said probe tube, said zoom motor is disposed inside said handle housing, said probe tube has a probe channel therein, wherein:

the collimating lens, the analyzer, the polarizing beam splitter, the second quarter wave plate, the plane reflector, the third quarter wave plate, the scanning galvanometer, the scanning mirror and the dichroic mirror in the first light path are all located in the handle shell, the relay lens and the objective lens in the first light path are all located in the detection channel, and the objective lens is located at a channel opening of the detection channel.

6. The variable focus cavity endoscope apparatus of claim 2, further comprising an electrically adjustable curvature lens between said collimating lens and said analyzer, wherein said pulsed laser signal transmits said collimating lens, said electrically adjustable curvature lens, said analyzer, said polarizing beamsplitter, said first quarter wave plate to said scanning galvanometer forming a new first light ray.

7. The variable focus endoscope probing apparatus according to claim 3, further comprising an electrically adjustable curvature lens disposed between said collimating lens and said analyzer, wherein said pulsed laser signal is transmitted through said collimating lens, said electrically adjustable curvature lens, said analyzer and said polarization beam splitter to form a new first light beam.

8. The variable focus endoscope probe device according to claim 4, wherein a plurality of illumination channels are further provided in the probe tube, and illumination fiber bundles for transmitting illumination light signals are provided in the illumination channels, wherein the illumination channels are uniformly distributed around the axial center of the probe channel.

9. The variable focus cavity endoscopic detection device as claimed in claim 8, wherein an observation channel is further disposed within said detection tube, said observation channel being located between said detection channel and said illumination channel, wherein:

and an observation lens is arranged at the channel opening of the observation channel and is connected with the bright field optical fiber bundle in the observation channel so as to acquire image information of the tissue area to be detected in front of the objective lens.

10. A three-dimensional nonlinear laser scanning cavity endoscope is characterized by comprising:

a fluorescence collection device, a scan acquisition controller, a femtosecond pulse laser, an optical fiber coupling module, and the variable-focus cavity endoscope detection device according to any one of claims 1 to 9, wherein the fluorescence collection device and the optical fiber coupling module are both in optical fiber communication connection with the variable-focus cavity endoscope detection device, and the fluorescence collection device and the variable-focus cavity endoscope detection device are both electrically connected with the scan acquisition controller, wherein: