CN109744983A - Zoom-type cavity endoscope detection device and laser scanning cavity endoscope - Google Patents
Zoom-type cavity endoscope detection device and laser scanning cavity endoscope Download PDFInfo
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Abstract
The embodiment of the present invention provides a kind of zoom-type cavity endoscope detection device and laser scanning cavity endoscope.Wherein, above-mentioned zoom-type cavity endoscope detection device includes the handle housing moved up and down relatively by the first transmission ring and the second transmission ring and detection pipes, is provided with the first optical path including collimation lens, micro electromechanical scanning galvanometer, lens, dichroscope, relay lens and object lens and the second optical path including object lens, relay lens and dichroscope in the middle siphunculus of handle housing.Varifocal cavity endoscope detection device provided in an embodiment of the present invention and laser scanning cavity endoscope pass through moving up and down between transmission ring, detection pipes and handle housing are driven to move up and down relatively, so that the light channel structure in handle housing can carry out zoom operation, realize the cell imaging of the different depths such as gastrointestinal tissue, oral cavity tissue and uterine cavity inner tissue in human abdominal cavity, whether there is or not Cancer residual equicorrelated cases for invasive depth, transfer case and the surgical operation incisxal edge of accurate judgement tumour.
Description
Technical field
The present embodiments relate to laser scanning endoscopic technique fields more particularly to a kind of zoom-type cavity endoscope to visit
Survey device and laser scanning cavity endoscope.
Background technique
Gastrointestinal cancer is to induce the second largest reason of developed country crowd cancer stricken death, and in recent years should
Trend is more and more obvious.Surgery radical operation is mainly used for the treatment of gastrointestinal cancer, but is embodied outer
When section's radical operation it needs to be determined that the operation excision specific range, therefore carry out operation consent, it is to be understood that tumour
Whether there is or not Cancer residuals etc. for good pernicious, invasive depth, transfer case and incisxal edge.Therefore biopsy is swollen for gastrointestinal tract under preoperative Gastrointestinal Endoscopes
Tumor tissue diagnosis is a critically important diagnostic evidence.And according to tumorous size, growth position, invasive depth etc., by gastric cancer
Art formula be divided into that stomach is cut entirely, stomach time is cut entirely, gastric resection and endoscopic inferior mucosa or submucous resection etc..
And at present, biopsy carries out iconography imaging usually based on Gastrointestinal Endoscopes supplemented by CT, MRI etc. under Gastrointestinal Endoscopes, or
Assessment is carried out to many gastrointestinal diseases with traditional white light laparoscope or endoscope to deposit.
But based on Gastrointestinal Endoscopes, the imaging of progress iconography haves the shortcomings that some inevitable supplemented by CT, MRI etc.,
For example be easy to cause intestinal tube or knurl bleeding in operation, need artificial drawing or squeeze, when Gastrointestinal Endoscopes cannot pass through intestinal tube,
Endoscopic biopsy is carried out repeatedly so that time delay, if causing severe haemorrhage also needs additional first aid hemostasis etc..And CT, MRI etc.
Complementary detection methods are unable to judge accurately the invasive depth and lymphatic metastasis of upper gastrointestinal road tumour in clinical practice
Situation.And gastroenteric tumor T is judged by stages by endoscopic ultrasonography, its accuracy of document report is only 44.7%~78%, insufficient
To become a reliable diagnostic criteria.Endoscopic ultrasonography is also ineffective to the preoperative judge of local resection operation, can not be accurate
Gastrointestinal mucosa level is segmented, and effect is also poor by stages to N.Therefore, just it is badly in need of one from the point of view of current gastrointestinal tract aided diagnosis technique
The new gastroenteric tumor diagnostic device of kind, with stomach intestinal tissue's information of real-time detection different depth in situ.
Summary of the invention
For the technical problems in the prior art, the embodiment of the present invention provides a kind of zoom-type cavity endoscope detection
Device and laser scanning cavity endoscope.
In a first aspect, the embodiment of the present invention provides a kind of zoom-type cavity endoscope detection device, comprising:
Handle housing and detection pipes, the handle housing bottom end are provided with middle siphunculus and the first transmission ring, the spy
Test tube top is provided with the second transmission ring, and first transmission ring and second transmission ring are rotatablely connected, and the middle siphunculus is embedding
It covers in the detection pipes and first transmission ring, zoom motor is provided in the handle housing and is used to form the
The light channel structure of one optical path and the second optical path, in which:
The zoom motor, for driving second transmission ring to move up and down relative to first transmission ring；
First optical path successively include collimation lens, micro electromechanical scanning galvanometer, lens, dichroscope, relay lens and
Object lens, wherein first optical path is for conducting the received laser signal of the collimation lens from the collimation lens to the object
Mirror；
Second optical path successively includes the object lens, the relay lens and the dichroscope, wherein described second
Optical path is for conducting the collected optical signal of the object lens from the object lens to the dichroscope.
Second aspect, the embodiment of the present invention provide a kind of three dimensional non-linear laser scanning cavity endoscope, comprising:
Phosphor collection device, scanning collection controller, femtosecond pulse laser, fiber coupling module and the present invention are real
Apply the zoom-type cavity endoscope detection device that a first aspect provides, the phosphor collection device and the fiber coupling module
It is connect with the zoom-type cavity endoscope detection device fiber optic communication, the phosphor collection device and the zoom-type cavity
Endoscope detection device is electrically connected with the scanning collection controller, in which:
The femtosecond pulse laser, for exporting pulsed laser signal to the fiber coupling module；
The fiber coupling module, for coupling the pulsed laser signal of the femtosecond pulse laser output, and
Transmit collimation lens described in the pulsed laser signal to the zoom-type cavity endoscope detection device；
The zoom-type cavity endoscope detection device exports the pulse after receiving the pulsed laser signal
The laser signal autofluorescence substance intracellular to life entity, and the autofluorescence substance excitation is obtained by the object lens
The fluorescence signal and second harmonic signal generated afterwards, and the fluorescence signal and the second harmonic signal are exported to the fluorescence
Collection device；
The phosphor collection device converts institute after receiving the fluorescence signal and the second harmonic signal respectively
It states fluorescence signal and the second harmonic signal is corresponding electric signal；
The scanning collection controller sweeps the pulsed laser signal for controlling the micro electromechanical scanning galvanometer
Retouch and synchronous acquisition described in electric signal.
Zoom-type cavity endoscope detection device laser scanning cavity endoscope provided in an embodiment of the present invention passes through zoom
Second transmission ring of motor driven detection pipes is moved up and down relative to the first transmission ring of handle housing, to realize that detection pipes are opposite
It include that the light channel structure being arranged in handle housing moves up and down in handle housing, so that the object lens in light channel structure can be opposite
It is moved up and down in detection device, to carry out zoom operation, Lai Shixian gastrointestinal tissue, oral cavity tissue and palace in human abdominal cavity
When intraluminal tissue is detected, the histocyte imaging of different depth can be carried out, to obtain the structure of histocyte different depth
Information, to more accurately judge the invasive depth of tumour, transfer case and surgical operation incisxal edge, whether there is or not the phases such as Cancer residual
Concern feeling condition, easy to operate, easy to use.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is this hair
Bright some embodiments for those of ordinary skill in the art without creative efforts, can be with root
Other attached drawings are obtained according to these attached drawings.
Fig. 1 is zoom-type cavity endoscope detection device structural schematic diagram one provided in an embodiment of the present invention；
Fig. 2 is zoom-type cavity endoscope detection device structural schematic diagram two provided in an embodiment of the present invention；
Fig. 3 is zoom-type cavity endoscope detection device structural schematic diagram three provided in an embodiment of the present invention；
Fig. 4 is zoom-type cavity endoscope detection device structural schematic diagram four provided in an embodiment of the present invention；
Fig. 5 is three dimensional non-linear laser scanning cavity endoscope structure schematic diagram one provided in an embodiment of the present invention；
Fig. 6 is three dimensional non-linear laser scanning cavity endoscope structure schematic diagram two provided in an embodiment of the present invention；
Fig. 7 is phosphor collection apparatus structure schematic diagram provided in an embodiment of the present invention；
Fig. 8 is the envelope of the box composite structure of three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention
Box structure schematic diagram one；
Fig. 9 is the envelope of the box composite structure of three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention
Box structure schematic diagram two；
Figure 10 provides the mesa structure schematic diagram one of three dimensional non-linear laser scanning cavity endoscope for the embodiment of the present invention；
The mesa structure schematic diagram two of Figure 11 embodiment of the present invention offer three dimensional non-linear laser scanning cavity endoscope.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art
Every other embodiment obtained without creative efforts, shall fall within the protection scope of the present invention.
At present based on Gastrointestinal Endoscopes, iconography imaging is carried out supplemented by CT, MRI etc. to obtain the good pernicious, leaching of tumour
Moisten depth, transfer case and incisxal edge whether there is or not relevant informations such as Cancer residuals, have the shortcomings that in concrete operations, for example is easy
Lead to intestinal tube or knurl bleeding, need artificial drawing or squeeze, when Gastrointestinal Endoscopes cannot pass through intestinal tube, carries out endoscopic biopsy repeatedly
So that time delay, if causing severe haemorrhage also needs additional first aid hemostasis etc..And the complementary detection methods such as CT, MRI,
The invasive depth and lymphatic metastasis situation of upper gastrointestinal road tumour are unable to judge accurately in clinical practice.And by ultrasound
Mirror judges gastroenteric tumor T by stages, its accuracy of document report is only 44.7%~78%, and being not enough to, which becomes one, reliably examines
Disconnected standard, and endoscopic ultrasonography is ineffective to the preoperative judge of local resection operation, can not precisely subdivided gastrointestinal mucosa level, and
To N, effect is also poor by stages.
And traditional white light laparoscope can assess many gastrointestinal diseases with endoscope, but the technology is only limitted to examine
Survey general form variation.Although being easy to find suspicious region, compared with In vivo detection technology, these technologies and false positive rate
And specificity etc. is related.White light endoscopy is associated with the extensive error that micro-variations diagnose, including ulcerative colitis
Or the inspection diagnosis including Barrett oesophagus and Flat Adenoma depauperation.Confocal endoscope combination laser technology, fluorescence are visited
Survey technology, fast scanning techniques etc. are because that can detect mucous membrane variation in microscopic scale, it is possible to for replacing tissue biopsy, and by
To extensive concern, which has highly sensitive and specificity.But be copolymerized burnt based endoscopic imaging technology still by
As the limitation of depth and fluorescent dye, since stomach and intestine sample has very strong absorption and scattering, imaging depth only to exist visible light
Superficial layer, and it also requires injecting specific fluorescent staining developer, operation is excessively complicated, cannot accurately obtain the leaching of tumour
Moistening depth, transfer case and surgical operation incisxal edge, whether there is or not the relevant informations such as Cancer residual.
And two-photon micro-imaging technique uses the longer femtosecond pulse laser of wavelength to have imaging as excitation light source
The features such as depth is deep, light injury is small, photobleaching region is small, phosphor collection is high-efficient, has in the imaging deep to biological tissue
There is epoch-making meaning.It is micro- that the W.Denk et al. of nineteen ninety Cornell University has developed First two-photon fluorescence in the world
Mirror, using the multi-photon micro-imaging technique based on nonlinear optics and femtosecond pulse.The technology is by utilizing living body
The second harmonic that the autofluorescence and collagen tissue that cell itself generates in tissue generate, can obtain sample real-time, quickly
Institutional framework and cellular morphology.Early in 1986, second harmonic was used for skin research and the research of coronary artery micro-imaging, card
Its real feasibility that be used to observe biological tissue.MPM also can be used as an important tool of cancer research.Cell itself produces
Raw autofluorescence from intracellular nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) (FAD),
It is 460nm that NADH, which issues wavelength, and the secondary oscillation harmonic wave of collagen is 370~390nm, so leading to when observing tumor specimen tissue
Often select the multiphoton microscope of 780~940nm range.MPM imaging is not only suitable with the tumor tissue pathology of standard, simultaneously
The additional information of tumor neogenetic process is also provided, such as can reflect the metabolism of tumor tissue cell by surveying rate value NADH/FAD
It is horizontal.
Using multi-photon imaging technique, multiphoton microscope is capable of providing real-time stomach intestinal tissue's structure and cellular morphology
Learn information.Multi-photon imaging technique has without exogenous marker tissue, extremely sensitive to collagen, small to the light injury of tissue and wear
The features such as depth is deep thoroughly, may be applied to the optical biopsy of gastroenteric tumor.There is presently no useful clinically two-photon abdomens
Hysteroscope and endoscope, and the cavity endoscope detection device based on two photon imaging, with real-time detection stomach intestinal tissue in situ
Information.
For stomach intestinal tissue's information of real-time detection different depth in situ, the embodiment of the invention provides a kind of zoom-types
Cavity endoscope detection device, Fig. 1 are zoom-type cavity endoscope detection device structural schematic diagram provided in an embodiment of the present invention
One, as shown in Figure 1, the zoom-type cavity endoscope detection device includes:
Handle housing 11 and detection pipes 12,11 bottom end of handle housing are provided with middle siphunculus 13 and the first transmission ring 111,
12 top of detection pipes is provided with the second transmission ring 121, and the first transmission ring 111 and the second transmission ring 121 are rotatablely connected, middle siphunculus 13
It is nested in detection pipes 12 and the first transmission ring 111, zoom motor 119 is provided in handle housing 11 and is used to form the
The light channel structure of one optical path and the second optical path, in which:
Zoom motor 119, for driving the second transmission ring 121 to move up and down relative to the first transmission ring 111；
First optical path successively include collimation lens 113, micro electromechanical scanning galvanometer 114, lens 115, dichroscope 116, in
After mirror 117 and object lens 118, wherein the first optical path is for conducting the received laser signal of collimation lens 113 from collimation lens 113
To object lens 118；
Second optical path successively includes object lens 118, relay lens 117 and dichroscope 116, wherein the second optical path is for conducting
The collected optical signal of object lens 118 is from object lens 118 to dichroscope 116.
Specifically, zoom-type cavity endoscope detection device provided in an embodiment of the present invention includes handle housing 11 and visits
12 two primary structures of test tube, wherein have cavity inside handle housing 11, housing bottom has an opening, opening be provided in lead to
Pipe 13 has to form the light channel structure of the first optical path and the second optical path in handle housing 11, the first optical path include collimation lens 113,
Micro electromechanical scanning galvanometer 114, lens 115, dichroscope 116, relay lens 117 and object lens 118 are used for exciting human stomach group
Knit or oral tissue cell in autofluorescence substance laser signal, by collimation lens 113 in the first optical path, micro electronmechanical sweep
After retouching galvanometer 114, lens 115, dichroscope 116, relay lens 117 and object lens 118, autofluorescence is emitted to from object lens 118
On substance, after excitation autofluorescence substance generates two-photon signal and second harmonic signal, two-photon letter is collected by object lens 118
Number and second harmonic signal be collected into phosphor collection device and by the relay lens 117 and dichroscope 116 in the second optical path
In for obtaining the detection information of gastrointestinal tissue or oral cavity tissue to be measured, with judge the invasive depth of tumour, transfer case and
Whether there is or not the relevant informations such as Cancer residual for surgical operation incisxal edge, wherein is provided with the first transmission ring on the outside of 11 bottom end opening of handle housing
111, middle siphunculus 13 is nested in the first transmission ring 111, second transmission ring 121 and the first transmission ring 111 on 12 top of detection pipes
It relatively rotates and is cooperatively connected, the zoom motor 119 being set in handle housing 11 drives the second transmission ring 121 and the first transmission ring
111 move up and down including relative rotation mode relative to moving up and down and directly drive and vertically move, to realize 12 phase of detection pipes
Include light channel structure therein for handle housing 11, move up and down, so that entire light channel structure can be relative in detection pipes 12
The passway of detection channels moves up and down, and to realize the histocyte detection of different depth, obtains the eucaryotic cell structure of different depth
Information.
Wherein, the setting of relay lens 117 is conducted for long range for exciting autofluorescence substance in 118 inside of object lens
Laser signal is from dichroscope 116 to object lens 118, and the conduction collected two-photon signal of object lens 118 and second harmonic
Signal to dichroscope 116, the image planes of laser signal object lens 118 are overlapped with the focal plane of relay lens 117, will pass through micro electromechanical scanning
The laser signal scanning area of mirror is with ratio of 1:1 etc. than conducting to the image planes of object lens 118, and wherein relay lens 117 can root
According to specifically being extended or shortened.
Wherein, dichroscope 116, which can according to need to be set as growing, leads to short anti-dichroscope 116 or short elongated anti-dichroic
Mirror 116 transmits the pulsed laser signal for exciting autofluorescence substance when that is, setting length leads to short anti-dichroscope 116, reflects
The two-photon signal and second harmonic signal being collected into, as shown in Figure 1, at this point, the zoom-type cavity endoscope detection device
It can be the detection device of laparoscope or hysteroscope；Fig. 2 is zoom-type cavity endoscope detection device provided in an embodiment of the present invention
Structural schematic diagram two, as shown in Fig. 2, reflection is for exciting certainly when the dichroscope 116 is short elongated anti-dichroscope 116
Fluoresce the pulsed laser signal of substance, transmits the two-photon signal and second harmonic signal being collected into, the dichroscope 116
Reflect the laser signal being incident on dichroscope 116 after collimation lens 113, micro electromechanical scanning galvanometer 114, lens 115
By relay lens 117 to object lens 118, the collected two-photon signal of object lens 118 and second harmonic signal are transmitted, at this point, should
Zoom-type cavity endoscope detection device can be the detection device of mouth mirror.
Zoom-type cavity endoscope detection device provided in an embodiment of the present invention drives the of detection pipes by zoom motor
Two transmission rings are moved up and down relative to the first transmission ring of handle housing, to realize that detection pipes relative to handle housing include handle
The light channel structure being arranged in shell moves up and down, so that the object lens in light channel structure can be relative to moving down in detection device
Dynamic, to carry out zoom operation, Lai Shixian gastrointestinal tissue, oral cavity tissue and uterine cavity inner tissue in human abdominal cavity are detected
When, the histocyte imaging of different depth can be carried out, to obtain the structural information of histocyte different depth, thus more accurate
Ground judge the invasive depth, transfer case and surgical operation incisxal edge of tumour whether there is or not Cancer residual equicorrelated case, it is easy to operate, make
With conveniently.
On the basis of the various embodiments described above, in zoom-type cavity endoscope detection device provided in an embodiment of the present invention
It is provided with detection channels in detection pipes, path channels are provided in middle siphunculus, middle siphunculus is nested in detection channels, detection channels
Passway it is concordant with the passway of path channels, be provided with coverslip at the passway of detection channels, in which:
Collimation lens, micro electromechanical scanning galvanometer, lens, dichroscope, relay lens and the equal position of object lens in light channel structure
Between the fiber optic universal interface of handle housing and the passway of path channels；
Object lens and relay lens are all set in path channels, and object lens are located at the passway of path channels.I.e. the present invention is real
The middle siphunculus applied in the zoom-type cavity endoscope detection device of example offer is not only nested in the first transmission ring, is also nested in spy
It surveys in the detection channels and the second transmission ring of device, has path channels in middle siphunculus, be used to form the first optical path and the second light
The light channel structure on road is located between the intracorporal fiber optic universal interface of handle case and the passway of path channels, fiber optic universal interface pair
The outer various transmission fibers of connection, wherein the object lens and relay lens that the first optical path and the second optical path share are all set in path channels
It is interior, since the passway of detection channels and the passway of path channels are concordant, so being located at the object at the passway of path channels
Mirror also is located at the passway of detection channels simultaneously, so that optical path is logical when detection pipes are moved up and down relative to handle housing
The object lens being arranged at the passway in road can be moved up and down relative to the coverslip being arranged at detection channels mouth, so be needed with factually testing
It wants, can adjust the distance between object lens and coverslip, to carry out the histocyte imaging of different depth, to obtain histocyte not
With the structural information of depth, to more accurately judge that the invasive depth of tumour, transfer case and surgical operation incisxal edge have
It is easy to operate, easy to use without Cancer residual equicorrelated case.
On the basis of the various embodiments described above, in zoom-type cavity endoscope detection device provided in an embodiment of the present invention
Light channel structure further includes liquid lens, and Fig. 3 is that zoom-type cavity endoscope detection device structure provided in an embodiment of the present invention is shown
It is intended to three, as shown in figure 3, liquid lens 110 is positioned at collimation lens 113 and micro electromechanical scanning galvanometer 114 between, to be formed newly
First optical path, the first new optical path successively includes collimation lens 113, liquid lens 110, micro electromechanical scanning galvanometer 114, lens
115, dichroscope and object lens 118.I.e. liquid lens 110 setting so that, can by liquid lens 110 apply voltage or
Person's electric current makes 110 surface of liquid lens generate the bending accordingly arrived, and then the directional light that collimation lens 113 are emitted generates difference
Focal power.Specific optical path are as follows: laser signal is incident on liquid lens after collimation lens 113 from fiber exit in parallel
110, corresponding focal power is generated from liquid lens 110 according to the voltage or current signal of load, outgoing is converging or diverging with light
By micro electromechanical scanning galvanometer 114, lens, dichroscope, converged on sample after relay lens 117 is transmitted to object lens 118.
Wherein, the power variation that liquid lens 110 introduces can make the focus of the laser signal of 118 mouthfuls of object lens outgoing in longitudinal direction
It is moved forward and backward, and the response speed of liquid lens 110 is very fast, scan frequency may be implemented quickly in KHz magnitude
The scanning imagery of longitudinal direction.Wherein, liquid lens 110 is equivalent to parallel flat glass when not applying voltage or current signal
Glass without focal power and will not make the focus after object lens 118 generate any offset laser signal, thus realize 3 D stereo at
Picture.When specifically used, the liquid lens 110 is complementary with zoom motor 119, adjusts 118, object lens by zoom motor 119
It sets, after coarse adjustment to corresponding depth position, system is switched to 110 zoom scan mode of liquid lens, carries out to sample quick
Three-dimensional imaging, wherein when zoom-type cavity endoscope detection device is not when installing zoom motor 119, only by liquid
Lens 110 can also carry out zoom adjustment.
On the basis of the various embodiments described above, in zoom-type cavity endoscope detection device provided in an embodiment of the present invention
Several illumination channels are additionally provided in detection pipes, Fig. 4 is zoom-type cavity endoscope detection device provided in an embodiment of the present invention
Structural schematic diagram four, as shown in figure 4, it is provided with the illumination fiber optic bundle for being used for transmission illumination optical signal in illumination channel 123, wherein
Illumination channel 123 is uniformly distributed centered on the axle center of detection channels.It is peeped in zoom-type cavity i.e. provided in an embodiment of the present invention
Several illumination channels 123 are additionally provided in detection pipes in mirror detection device, 123 more than one of illumination channel, each channel
It is inside both provided with illumination fiber optic bundle, lighting fiber has certain pore size angle, does not need lens and be used directly for divergent illumination, and
Illumination channel 123 is uniformly distributed centered on the axle center of detection channels, is provided uniformly for zoom-type cavity endoscope detection device
Illumination, to facilitate the test serum zone state before work viewing objective.
On the basis of the various embodiments described above, in zoom-type cavity endoscope detection device provided in an embodiment of the present invention
Observation channel is additionally provided in detection pipes, as shown in figure 4, observation channel is located between detection channels and illumination channel, in which:
It observes at the passway in channel and is provided with observation camera lens 122, the light field optical fiber in observation camera lens 122 and observation channel
Beam is connected, to obtain the test serum regional image information before object lens.Zoom-type cavity i.e. provided in an embodiment of the present invention
Be additionally provided in detection pipes in endoscope detection device observation channel, the observation channel be located at detection channels with illuminate channel it
Between, and be provided with observation camera lens 122 and light field fiber optic bundle, light field fiber optic bundle be imaging optical fiber bundle, for transmit observation camera lens
Test serum regional image information before 122 object lens captured, wherein observation channel can be one, or two shapes
Three-dimensional light field cavity endoscope function is realized in visual observation in pairs.
On the basis of the various embodiments described above, in zoom-type cavity endoscope detection device provided in an embodiment of the present invention
Sorption channel is additionally provided in detection pipes, as shown in figure 4, sorption channel 124 is located between illumination channel and detection tube edges.I.e.
It is additionally provided in detection pipes in zoom-type cavity endoscope detection device provided in an embodiment of the present invention for making zoom-type chamber
Internal sight glass detection device is adsorbed on the sorption channel 124 in test serum, by extracting the air in sorption channel 124,
Negative pressure is formed in sorption channel 124, so that zoom-type cavity endoscope detection device is adsorbed in test serum, wherein absorption
Channel 124 is located between illumination channel and detection tube edges, that is, is located on the outside of illumination channel, close to the position of detection pipes avris
Place.
On the basis of the various embodiments described above, in zoom-type cavity endoscope detection device provided in an embodiment of the present invention
Button hole is offered on handle housing, switching push button and imaged button is provided in button hole, switching push button is for switching difference
Optical filter, to obtain the illumination optical signal of different wave length；
Imaged button be used to control the image-forming module that is connected with light field fiber optic bundle to the test serum region before object lens into
Row imaging.Setting in the button hole of handle housing in zoom-type cavity endoscope detection device i.e. provided in an embodiment of the present invention
There is switching push button, the optical filter of optical signal can be illuminated with switch filtering different wave length by the switching push button, so that staff
It can choose the illumination optical signal transmitted through the different wave length come；Handle housing in zoom-type cavity endoscope detection device
It is provided with imaged button in button hole, the image-forming module being connected with light field fiber optic bundle can control to object by the imaged button
Test serum region before mirror carries out imaging of taking pictures, and wherein the function of switching push button and imaged button can also be made by oneself by software
Justice modifies its corresponding function.
The embodiment of the present invention also provides a kind of three dimensional non-linear laser scanning cavity endoscope, and Fig. 5 is the embodiment of the present invention
The three dimensional non-linear laser scanning cavity endoscope structure schematic diagram one of offer, as shown in figure 5, the three dimensional non-linear laser scanning
Cavity endoscope includes:
Phosphor collection device 56, scanning collection controller 531, femtosecond pulse laser, fiber coupling module and above-mentioned
The zoom-type cavity endoscope detection device 1 that each embodiment provides, phosphor collection device 56 and fiber coupling module are and zoom
1 fiber optic communication of formula cavity endoscope detection device connection, phosphor collection device and zoom-type cavity endoscope detection device with
Scanning collection controller 531 is electrically connected, in which:
Femtosecond pulse laser, for exporting pulsed laser signal to fiber coupling module；
Fiber coupling module, for coupling the pulsed laser signal of femtosecond pulse laser output, and transmission pulse laser
Signal collimation lens into zoom-type cavity endoscope detection device 1；
Zoom-type cavity endoscope detection device 1, after receiving pulsed laser signal, output pulsed laser signal to life
The autofluorescence substance in body cell is ordered, and obtains the fluorescence signal and two generated after the excitation of autofluorescence substance by object lens
Rd harmonic signal, and fluorescence signal and second harmonic signal are exported to phosphor collection device 56；
Phosphor collection device 56, after receiving fluorescence signal and second harmonic signal, difference conversion fluorescence signal and two
Rd harmonic signal is corresponding electric signal；
Scanning collection controller 531 is scanned pulsed laser signal for controlling micro electromechanical scanning galvanometer, Yi Jitong
Step acquisition electric signal.
Specifically, three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention includes phosphor collection device
56, scanning collection controller 531, femtosecond pulse laser, fiber coupling module and zoom-type cavity endoscope detection device
1, swashed to be formed using the three dimensional non-linear that two photon imaging technology detects human body gastrointestinal tissue and oral cavity tissue
Optical scanning cavity endoscope, wherein femtosecond pulse laser can be used for exciting human gastrointestinal tissue with emission pulse laser signal
And the autofluorescence substance in oral tissue cell, multiphoton fluorescence signal and second harmonic signal are generated, including use
FAD and collagen in the femtosecond pulse laser activated cell of 920nm excite the fluorescence signal and 460nm of 500-600nm
Second harmonic signal, and pass through 780nm femtosecond pulse laser activated cell in FAD or the autofluorescences object such as NADH
Matter, to generate corresponding fluorescence signal and second harmonic signal, wherein femtosecond pulse laser and fiber coupling module combination exist
It is formed together laser emitting module 540；
Wherein, phosphor collection device 56 is integrated with two paths of signals and collects optical path, respectively fluorescence signal collection optical path and two
Rd harmonic signal collects optical path, the collection respectively of Lai Shixian fluorescence signal and second harmonic signal；Scanning collection controller 531 is controlled
Micro electromechanical scanning galvanometer processed is scanned pulsed laser signal and autofluorescence substance is excited to generate fluorescence signal and secondary humorous
Wave signal, and the first electric signal and the second electricity that acquisition phosphor collection device conversion fluorescence signal and second harmonic signal obtain
Signal；The three dimensional non-linear laser scanning cavity endoscope, can according to the difference of 1 structure of zoom-type cavity endoscope detection device
It is divided into laparoscope and mouth mirror.Wherein, the resolution ratio of the three dimensional non-linear laser scanning cavity endoscope may be configured as 800nm,
Visual field can be 400 microns * 400 microns, and image taking speed can be 26 frames (256*256 pixel) or 13 frames (512*512 pixel).
Three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention is adopted using phosphor collection device, scanning
Collect controller, femtosecond pulse laser, fiber coupling module and zoom-type cavity endoscope detection device, to form utilization
The laser scanning cavity endoscope that two photon imaging technology detects human body gastrointestinal tissue and oral cavity tissue, passes through liquid
Lens and zoom motor adjust objective focal length, realize that laser scanning microscope carries out three-dimensional to the eucaryotic cell structure of different depth and sweeps
It retouches, multiphoton fluorescence signal and second harmonic signal is obtained by autofluorescence substance in femtosecond pulse laser activated cell,
It realizes that laser scanning microscope is non-linear, fluorescence signal and second harmonic signal is collected by phosphor collection device, and be converted to
Corresponding electric signal, and then the corresponding fluorescent image etc. for reflecting cell tissue structure is obtained by the electric signal, wherein become
The use of burnt formula cavity endoscope detection device allows staff flexibly to gastrointestinal tissue, oral cavity group in human abdominal cavity
It knits and uterine cavity inner tissue is detected, when being detected to human body gastrointestinal tissue, human abdomen need to only be opened up one small
Mouthful, the pain of operation cost and patient is so reduced, when uterine cavity inner tissue is imaged, nature channel (vagina) can be passed through
Non-invasive detection is carried out, equipment operation is simple, easy to use.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror, further includes lighting module and image-forming module, as shown in figure 5, lighting module 534 and image-forming module 533 with zoom-type chamber
Internal sight glass detection device fiber optic communication connection, in which:
Lighting module 534 successively includes illuminating lens 5342, variable filter 5341 and lighting source 5343, illuminating lens
5342 are connected with illumination fiber optic bundle, and lighting source is for providing illumination optical signal；
Image-forming module 533 successively includes imaging len 5331 and camera 5332, imaging len 5331 and light field fiber optic bundle phase
Connection, camera 5332 is for obtaining test serum regional image information.Three dimensional non-linear laser i.e. provided in an embodiment of the present invention
Scanning cavity endoscope is additionally provided with lighting module 534 and image-forming module 533, wherein lighting module 534 successively includes illumination
Lens 5342, variable filter 5341 and lighting source 5343, wherein lighting source passes through electric variable optical filter runner, can
To switch different optical filters, to obtain the illumination optical signal of different wave length, basic principle is not interfere two-photon fluorescence imaging,
Such as obtain autofluorescence and when second harmonic, red or infrared optical filter can be switched to, with obtain 370nm,
The illumination optical signal of 635nm or infrared 850nm, 940nm, illumination optical signal enter illumination fiber optic bundle by Lens Coupling；
Image-forming module 533 includes successively imaging len and camera, and lens focus is imaged on camera, bright for directly observing
?.Wherein camera is two corresponding with binocular light field fiber optic bundle, and light field imaging and two photon imaging form multi-modal laparoscope, bright
Field binocular three-dimensional stereo laparoscope mode, carries out wide-field sample view, the basic pattern of main detection sample.It can for having
Doubtful or interested region, can switch to two-photon mode, carries out autofluorescence and Second Harmonic Imaging, observes the cell of sample
Grade form provides foundation for further judgement, and wherein camera can set for the imaging based on image devices such as CCD or CMOS
It is standby.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror further includes air extractor, as shown in figure 5, air extractor 52 includes mainly aspiration pump, passes through exhaust pipe and sorption channel phase
Even, extraction valve is set in exhaust pipe, extraction valve is electrically connected with air extractor 52, and air extractor 52 is opened by adjusting extraction valve
The size closed and be opened and closed, controls the extraction flow of exhaust pipe, to realize that the pumping to sorption channel controls, and then adjusts
Negative pressure in sorption channel, so that zoom-type cavity endoscope detection device is adsorbed on human abdominal cavity by the effect of atmospheric pressure
The tissues such as interior stomach and intestine, oral cavity and uterine cavity reduce bio-tissue activity bring motion artifacts, so that imaging is more steady
It is fixed, clear.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror further includes industrial personal computer, as shown in figure 5, industrial personal computer 532 is electrically connected with scanning collection controller 531, in which:
Industrial personal computer 532 is for obtaining collected first electric signal of scanning collection controller 531 and the second electric signal, and base
The first fluorescent image is generated in the first electric signal and the second fluorescent image is generated based on the second electric signal.That is the embodiment of the present invention
The three dimensional non-linear laser scanning cavity endoscope of offer further includes the industrial personal computer 532 being electrically connected with scanning collection controller 531,
The industrial personal computer 532 is based on the first electric signal and generates the first fluorescent image and generate the second fluorescent image based on the second electric signal,
It can be respectively used to display eucaryotic cell structure and fibre structure information, control software is wherein installed on industrial personal computer, by controlling software,
Control instruction is sent to scanner, to control scanning collection controller, to obtain above-mentioned first electric signal and the second electric signal.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror further includes display, as shown in figure 5, display 55 is electrically connected with industrial personal computer 532, for showing the first fluorescent image and the
Two fluorescent images.Three dimensional non-linear laser scanning cavity endoscope i.e. provided in an embodiment of the present invention further includes for showing first
The display 55 of fluorescent image and the second fluorescent image, by display 55, staff can directly acquire the first fluorogram
The relevant information of picture and the second fluorescent image.
Wherein, Fig. 6 is three dimensional non-linear laser scanning cavity endoscope structure schematic diagram two provided in an embodiment of the present invention,
As shown in fig. 6, the three dimensional non-linear laser scanning cavity endoscope also includes:
Phosphor collection device 56, scanning collection controller 531, femtosecond pulse laser, fiber coupling module and above-mentioned
The zoom-type cavity endoscope detection device 1 of each embodiment offer, air extractor 52, industrial personal computer 532, lighting module 534, imaging
Module 533, phosphor collection device 56 and fiber coupling module connect with 1 fiber optic communication of zoom-type cavity endoscope detection device
It connecing, phosphor collection device 56 and zoom-type cavity endoscope detection device 1 are electrically connected with scanning collection controller 531, wherein
Each part mentioned above module or the function of device are identical as the apparatus function effect in the various embodiments described above, including femtosecond pulse
Device and fiber coupling module are grouped together into laser emitting module 540, and lighting module 534 successively includes illuminating lens
5342, variable filter 5341 and lighting source 5343, image-forming module 533 successively include imaging len and camera, and the three-dimensional is non-
It is mouth mirror detection device, the zoom-type that linear laser, which scans the zoom-type cavity endoscope detection device 1 in cavity endoscope,
Liquid lens, the liquid for including in effect and the various embodiments described above are contained in the light channel structure of cavity endoscope detection device
Body lens are identical, and optical path is also identical with optical path corresponding in the various embodiments described above.
On the basis of the various embodiments described above, Fig. 7 is phosphor collection apparatus structure schematic diagram provided in an embodiment of the present invention,
As shown in fig. 7, phosphor collection device provided in an embodiment of the present invention includes collecting fiber optic universal interface 881, the first photomultiplier transit
Pipe 882, the second photomultiplier tube 883 and positioned at collect between fiber optic universal interface 881 and the first photomultiplier tube 882 the
One collects optical path, collects optical path positioned at second collected between fiber optic universal interface 881 and the second photomultiplier tube 883, in which:
First collection optical path successively includes coupled lens 81, infrared fileter 82, the first dichroscope 83, the first optical filter
84 and first collecting lens 85, wherein the first collection optical path is for collecting the fluorescence signal that phosphor collection device receives, and the
One photomultiplier tube 882 is the first electric signal for conversion fluorescence signal；
Second collection optical path successively includes coupled lens 81, infrared fileter 82, the first dichroscope 83, the second dichroic
Mirror 86, the second optical filter 87 and the second collecting lens 88, wherein the second collection optical path is for collecting the reception of phosphor collection device
The second harmonic signal arrived, the second photomultiplier tube 883 is for converting second harmonic signal as the second electric signal.I.e. the present invention is real
The phosphor collection device for applying example offer has simple two-way signal collecting function, is integrated with two-way optical path, wherein first collects in optical path
The first dichroscope 83 be transmission fluorescence signal, the dichroscope of reflected second harmonics, the second dichroscope 86 and the one or two
It is same dichroscope to Look mirror 83, is used for reflected second harmonics, the first optical filter 84 filters out it for transmiting fluorescence signal
Remaining interference signal, the second optical filter 87 filters out remaining interference signal for transmiting corresponding second harmonic signal, for example, using
When autofluorescence substance in the femto second optical fiber laser exciting human abdominal cavity 780nm or Stomatocyte, the secondary of 390nm can be obtained
The two-photon auto flourescence signals of harmonic signal and 450-600nm, are passed through by 420nm above wavelength, 420 or less wavelength reflections
Dichroscope i.e. the first dichroscope 83 can separate two-way fluorescence, respectively use 390 ± 20nm 84 He of the first optical filter
The second optical filter 87 available clean second harmonic signal and fluorescence signal of 450-600nm.
Wherein, Fig. 8 is the box combination knot of three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention
The joint sealing structural schematic diagram one of structure, as shown in figure 8, it is integrated to be integrated in display 55 and the cabinet for being equipped with modules on case lid
Together, facilitate whole equipment mobile, and replacement workplace, and the display 55 is when in use, case can be placed in addition
On body, to facilitate staff to obtain the information on display, wherein in the three dimensional non-linear laser scanning cavity endoscope
Zoom-type cavity endoscope detection device 1 is mouth mirror detection device.When having used in the three dimensional non-linear laser scanning cavity
After sight glass, staff can portable equipment case, convenient changing workplace uses especially in hospital, laboratory or outdoor location
The equipment can be more convenient.
Wherein, Fig. 9 is the box combination knot of three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention
The joint sealing structural schematic diagram two of structure, as shown in figure 9, it is integrated to be integrated in display 55 and the cabinet for being equipped with modules on case lid
Together, facilitate whole equipment mobile, and replacement workplace, and the display 55 is when in use, case can be placed in addition
On body, to facilitate staff to obtain the information on display, wherein in the three dimensional non-linear laser scanning cavity endoscope
Zoom-type cavity endoscope detection device 1 be laparoscope detection device, and laparoscope detection device can be arranged simultaneously it is multiple.When
After having used the three dimensional non-linear laser scanning cavity endoscope, staff can portable equipment case, convenient changing workplace,
It, can be more convenient using the equipment especially in hospital, laboratory or outdoor location.
On the basis of the various embodiments described above, three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention
In zoom-type cavity endoscope detection device be it is multiple.Phosphor collection device i.e. provided in an embodiment of the present invention and fiber coupling
Module can be connect with multiple zoom-type cavity endoscope detection device fiber optic communications simultaneously, i.e., sweep in a three dimensional non-linear laser
It retouches and integrates multiple detection devices in cavity endoscopic system, to be detected while realization to gastrointestinal tissue different parts, thus into
Row comparative analysis.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror further includes adjusting optical fiber, detects and fills with zoom-type cavity endoscope respectively for phosphor collection device and fiber coupling module
Optical fiber between setting transmits connection, in which:
Adjust the adjustable in length of optical fiber.Three dimensional non-linear laser scanning cavity endoscope i.e. provided in an embodiment of the present invention
In phosphor collection device and fiber coupling module pass through length-adjustable adjusting optical fiber and zoom-type cavity endoscope respectively and visit
Device progress optical fiber transmission connection is surveyed to carry out flexible movement detector to realize according to different experiments scene needs, avoided
Limit the limitation of fiber lengths, wherein the adjustable in length of optical fiber is adjusted, to realize various by the optical fiber for replacing different length
The application of occasion can carry out the optical fiber replacement of different length at any time as needed.
For the three dimensional non-linear laser scanning cavity endoscope that the various embodiments described above provide, the embodiment of the present invention is also provided
Another specific embodiment, Figure 10 provide the platform of three dimensional non-linear laser scanning cavity endoscope for the embodiment of the present invention
Formula structural schematic diagram one, as shown in Figure 10, the three dimensional non-linear laser scanning cavity endoscope include that air extractor 52, first fills
Set 53, second device 54, display 55 and zoom-type cavity endoscope detection device 1, wherein be integrated in first device 53
Scanning collection controller and industrial personal computer, industrial personal computer are electrically connected with display 55, second device 54 be integrated with femtosecond pulse laser,
Fiber coupling module and phosphor collection device, lighting module and image-forming module, fiber coupling module and phosphor collection device are equal
It transmits and connects with 51 optical fiber of absorption type microscope detection device, wherein zoom-type cavity endoscope detection device 1 is mouth mirror spy
Survey device, for detecting human oral cavity tissue, with understand good pernicious, invasive depth, transfer case and the incisxal edge of tumour whether there is or not
The information such as Cancer residual, the absorption type three dimensional non-linear laser scanning microscope working principle is identical as the various embodiments described above, herein
It repeats no more.
Wherein, Figure 11 embodiment of the present invention provides the mesa structure schematic diagram of three dimensional non-linear laser scanning cavity endoscope
Two, as shown in figure 11, which also includes air extractor 52, first device 53, second
Device 54, display 55 and zoom-type cavity endoscope detection device 1, wherein be integrated with scanning collection in first device 53
Controller and industrial personal computer, industrial personal computer are electrically connected with display 55, and second device 54 is integrated with femtosecond pulse laser, fiber coupling
Module and phosphor collection device, lighting module and image-forming module, fiber coupling module and phosphor collection device are and absorption type
The transmission connection of 51 optical fiber of microscope detection device, wherein zoom-type cavity endoscope detection device 1 is laparoscope detection device,
The laparoscope detection device be embedded in human abdomen, gastrointestinal tissue is detected, with understand tumour good pernicious, invasive depth,
Whether there is or not the information such as Cancer residual for transfer case and incisxal edge, and the laparoscope furthermore based on the laparoscope detection device can be used for pair
Women carries out the detection of uterine cavity inner tissue, and detection imaging of tissue principle is identical as the cavity endoscope principle of the various embodiments described above.
Above-mentioned, although the foregoing specific embodiments of the present invention is described with reference to the accompanying drawings, not to invention protection scope
Limitation, those skilled in the art should understand that, based on the technical solutions of the present invention, those skilled in the art are not required to
The above is only preferred embodiment of the present application, are not intended to restrict the invention, for those skilled in the art, this
Application can have various modifications and variations.Within the spirit and principles of this application, made any modification, equivalent replacement,
Improve etc., it should be included within the scope of protection of this application.
The apparatus embodiments described above are merely exemplary, wherein unit can be as illustrated by the separation member
Or may not be and be physically separated, component shown as a unit may or may not be physical unit, i.e.,
It can be located in one place, or may be distributed over multiple network units.It can select according to the actual needs therein
Some or all of the modules achieves the purpose of the solution of this embodiment.Those of ordinary skill in the art are not paying creative labor
In the case where dynamic, it can understand and implement.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: it still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features；
And these are modified or replaceed, technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution spirit and
Range.
Claims (10)
1. a kind of zoom-type cavity endoscope detection device characterized by comprising
Handle housing and detection pipes, the handle housing bottom end are provided with middle siphunculus and the first transmission ring, the detection pipes
Top is provided with the second transmission ring, and first transmission ring and second transmission ring are rotatablely connected, and the middle siphunculus is nested in
In the detection pipes and first transmission ring, it is provided with zoom motor in the handle housing and is used to form the first light
The light channel structure on road and the second optical path, in which:
The zoom motor, for driving second transmission ring to move up and down relative to first transmission ring；
First optical path successively includes collimation lens, micro electromechanical scanning galvanometer, lens, dichroscope, relay lens and object lens,
Wherein first optical path is for conducting the received laser signal of the collimation lens from the collimation lens to the object lens；
Second optical path successively includes the object lens, the relay lens and the dichroscope, wherein second optical path
For conducting the collected optical signal of the object lens from the object lens to the dichroscope.
2. zoom-type cavity endoscope detection device according to claim 1, which is characterized in that be provided in the detection pipes
Detection channels are provided with path channels in the middle siphunculus, and the middle siphunculus is nested in the detection channels, and the detection is logical
The passway in road is concordant with the passway of the path channels, is provided with coverslip at the passway of the detection channels, in which:
The collimation lens, the micro electromechanical scanning galvanometer, the lens, the dichroscope, institute in the light channel structure
State relay lens and the object lens be respectively positioned on the handle housing fiber optic universal interface and the path channels passway it
Between；
The object lens and the relay lens are all set in the path channels, and the object lens are located at the channel of the path channels
At mouthful.
3. zoom-type cavity endoscope detection device according to claim 1 or claim 2, which is characterized in that the light channel structure is also
Including liquid lens, the liquid lens is between the collimation lens and the micro electromechanical scanning galvanometer, to be formed newly
First optical path, the first new optical path successively includes collimation lens, the liquid lens, the micro electromechanical scanning galvanometer, institute
State lens, the dichroscope and the object lens.
4. zoom-type cavity endoscope detection device according to claim 2, which is characterized in that also set up in the detection pipes
There are several illumination channels, the illumination fiber optic bundle for being used for transmission illumination optical signal is provided in the illumination channel, wherein the photograph
Bright channel is uniformly distributed centered on the axle center of the detection channels.
5. zoom-type cavity endoscope detection device according to claim 4, which is characterized in that also set in the detection pipes
It is equipped with observation channel, the observation channel is located between the detection channels and the illumination channel, in which:
Observation camera lens, the bright field light in the observation camera lens and the observation channel are provided at the passway in the observation channel
Fine beam is connected, to obtain the test serum regional image information before the object lens.
6. zoom-type cavity endoscope detection device according to claim 4, which is characterized in that also set in the detection pipes
It is equipped with sorption channel, the sorption channel is between the illumination channel and the detection tube edges.
7. zoom-type cavity endoscope detection device according to claim 5, which is characterized in that opened on the handle housing
Equipped with button hole, switching push button and imaged button are provided in the button hole, the switching push button is for switching different filters
Mating plate, to obtain the illumination optical signal of different wave length；
The imaged button is for controlling the image-forming module being connected with the light field fiber optic bundle to be measured group before the object lens
Tissue region is imaged.
8. a kind of three dimensional non-linear laser scanning cavity endoscope characterized by comprising
Phosphor collection device, scanning collection controller, femtosecond pulse laser, fiber coupling module and claim 1-7 appoint
Zoom-type cavity endoscope detection device described in one, the phosphor collection device and the fiber coupling module with it is described
Zoom-type cavity endoscope detection device fiber optic communication connection, the phosphor collection device and the zoom-type cavity endoscope are visited
Device is surveyed to be electrically connected with the scanning collection controller, in which:
The femtosecond pulse laser, for exporting pulsed laser signal to the fiber coupling module；
The fiber coupling module for coupling the pulsed laser signal of the femtosecond pulse laser output, and transmits
Collimation lens described in the pulsed laser signal to the zoom-type cavity endoscope detection device；
The zoom-type cavity endoscope detection device exports the pulse laser after receiving the pulsed laser signal
The signal autofluorescence substance intracellular to life entity, and produced after obtaining the autofluorescence substance excitation by the object lens
Raw fluorescence signal and second harmonic signal, and the fluorescence signal and the second harmonic signal are exported to the phosphor collection
Device；
The phosphor collection device is converted described glimmering respectively after receiving the fluorescence signal and the second harmonic signal
Optical signal and the second harmonic signal are corresponding electric signal；
The scanning collection controller is scanned the pulsed laser signal for controlling the micro electromechanical scanning galvanometer,
And electric signal described in synchronous acquisition.
9. three dimensional non-linear laser scanning cavity endoscope according to claim 8, which is characterized in that further include illumination mould
Block and image-forming module, the lighting module and the image-forming module connect with the cavity endoscope detection device fiber optic communication
It connects, in which:
The lighting module successively includes illuminating lens, variable filter and lighting source, the illuminating lens and lighting fiber
Beam is connected, and the lighting source is for providing illumination optical signal；
The image-forming module successively includes imaging len and camera, and the imaging len is connected with light field fiber optic bundle, the phase
Machine is for obtaining test serum regional image information.
10. three dimensional non-linear laser scanning cavity endoscope according to claim 8, which is characterized in that the fluorescence is received
Acquisition means include collecting fiber optic universal interface, the first photomultiplier tube, the second photomultiplier tube and being located at the collection optical fiber
First between general-purpose interface and first photomultiplier tube collects optical path, is located at the collection fiber optic universal interface and described
Second between second photomultiplier tube collects optical path, in which:
The first collection optical path successively includes coupled lens, infrared fileter, the first dichroscope, the first optical filter and the
One collecting lens, wherein the first collection optical path is described for collecting the fluorescence signal that phosphor collection device receives
First photomultiplier tube is for converting the fluorescence signal as the first electric signal；
The second collection optical path successively includes the coupled lens, the infrared fileter, first dichroscope, second
Dichroscope, the second optical filter and the second collecting lens, wherein the second collection optical path is for collecting phosphor collection device
The second harmonic signal received, second photomultiplier tube is for converting the second harmonic signal as the second telecommunications
Number.
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| CN113467071A (en) * | 2021-07-27 | 2021-10-01 | 郑州光超医疗科技有限公司 | Focusable in-vivo tissue high-resolution optical scanning probe |
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| CN113467071A (en) * | 2021-07-27 | 2021-10-01 | 郑州光超医疗科技有限公司 | Focusable in-vivo tissue high-resolution optical scanning probe |
| CN113467071B (en) * | 2021-07-27 | 2023-08-29 | 郑州光超医疗科技有限公司 | In-vivo tissue high-resolution optical scanning probe capable of focusing |
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