CN107456202A - A kind of nonlinear optics flexible endoscope imaging device - Google Patents
A kind of nonlinear optics flexible endoscope imaging device Download PDFInfo
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- CN107456202A CN107456202A CN201710924667.4A CN201710924667A CN107456202A CN 107456202 A CN107456202 A CN 107456202A CN 201710924667 A CN201710924667 A CN 201710924667A CN 107456202 A CN107456202 A CN 107456202A
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- 230000009021 linear effect Effects 0.000 claims abstract description 28
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- 238000010168 coupling process Methods 0.000 claims abstract description 27
- 238000005859 coupling reaction Methods 0.000 claims abstract description 27
- 238000001514 detection method Methods 0.000 claims abstract description 11
- 239000000463 material Substances 0.000 claims description 18
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- 229920000642 polymer Polymers 0.000 claims description 18
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- 238000009738 saturating Methods 0.000 claims description 10
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/273—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor for the upper alimentary canal, e.g. oesophagoscopes, gastroscopes
- A61B1/2736—Gastroscopes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/00163—Optical arrangements
- A61B1/00165—Optical arrangements with light-conductive means, e.g. fibre optics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/00163—Optical arrangements
- A61B1/00172—Optical arrangements with means for scanning
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/00163—Optical arrangements
- A61B1/00188—Optical arrangements with focusing or zooming features
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/005—Flexible endoscopes
Abstract
A kind of nonlinear optics flexible endoscope imaging device, it includes one group of coupled lens, multigroup relay lens, a piece of dichroscope, a piece of speculum, one group of fiber coupling lens, one group of object lens and an optical fiber.The ultrafast laser of two-dimensional scan is imported first group of relay lens by outside two-dimensional scanner by coupled lens, by that analogy, N groups relay lens continues two-dimensional scan plane in the air gap that is imaged between N groups relay lens and dichroscope, ultrafast laser is focused in external sample by dichroscope and by objective scan, collected in the non linear optical signal that focal point inspires by object lens, after dichroscope reflects 90 degree, 90 degree are reflected by speculum again, optical fiber is coupled into by fiber coupling lens and is finally transmitted to outside photoelectric detector.The present invention is simple in construction, and small volume, cost is low, can be used in combination with commercial gastroscope, and a variety of nonlinear properties such as TPEAF, SHG, CARS can detect simultaneously, realize and realize live body, functional imaging in situ, be expected to realize the detection of early-stage cancer.
Description
Technical field
It is real especially with the miniature relay lens of multi-disc the present invention relates to a kind of nonlinear optics flexible endoscope imaging device
Existing minor diameter flexible imaging light path, the distal end of the light path have miniature apochromatic objective, can be by outside two-dimensional scan
The instrument channel for the commercial endoscope that multi-wavelength's ultrashort pulse can be bent by distal end with small-bend radius focuses to
Human internal organs, so as to inspire the various non linear optical signals with diagnostic significance, and will be various non-thread by optical fiber
Property optical signalling is transmitted to external nonlinear optics detection device.The present invention can be used for the clinical noninvasive inspection for realizing early-stage cancer
Survey.
Background technology
Realization uses ultra-short pulse laser as excitation source, enters human consumption by the instrument channel of commercial electronic gastroscope
The scanning imaging system that road carries out nonlinear optics imaging is extremely difficult.Nonlinear optics scanning endoscopic imaging system first
General technological difficulties be：1. how intactly the ultrashort laser pulse as exciting light is not transferred into broadening
Go：Common nonlinear optical effect includes double/three-photon and excites fluorescence（Two/Third-photon Excited
Fluorescence, TPEF/Third PEF）, two-photon excitation autofluorescence（Two-photon Excited
Autofluorescence,TPEAF）, the generation of two/triple-frequency harmonics（Second/Third Harmonic Generation,SHG/
THG）, coherent anti-stokes raman scattering (Coherent Anti-Strokes Raman Scattering, CARS) etc..
Except needing double/three-photon of fluorochrome label to excite fluorescence not to be suitable for human body in these nonlinear optical effects, other
It is suitable for unmarked（Label free）In vivo detection.These nonlinear optical effect generally use femtosecond lasers（Double/tri- light
Son excites fluorescence, two-photon excitation autofluorescence, and two/triple-frequency harmonics occurs）Or picosecond laser（Coherent anti-Stokes Raman dissipates
Penetrate）As excitation source.The ultrashort laser pulse that light source is sent transmitted in the optical element such as ordinary optic fibre and lens mainly by
To Self-phase modulation（Self Phase Modulation, SPM）With group velocity delay (Group Velocity Delay,
GVD) both effects and broadening.Consider that there is flexible optical fiber first：Photonic crystal hollow optic fibre can
Ultrashort laser pulse is not transmitted in low-loss broadening, therefore has been applied to nonlinear optics scanning endoscopic imaging system；Imaging
Fibre bundle is made up of thousands of to tens thousand of single-mode fibers, because the diameter of every single-mode fiber is only a few micrometers, pulsewidth 100fs
Energy 10nJ laser pulse passes through 1cm length because self phase modulation is just stretched to 1- in single-mode fiber
10ps, peak energy substantially reduce.Energy is stronger, and self phase modulation is more obvious, and is difficult compensation.Laser arteries and veins after broadening
Punching continues to propagate in light can be based on broadening that group velocity delay effect is brought, and this broadening can be compensated.
The laser pulse of broadening can substantially reduce the launching efficiency of nonlinear effect.Therefore imaging optical fiber bundle has been used in copolymerization Jiao
Imaging is peeped, and is not used to nonlinear optics imaging.Secondly, up to the present, realized without any research using lens ultrashort sharp
Light pulse in the instrument channel of the electronic gastroscope of curved narrow intactly, not broadening（Or broadening can compensate for）Transmission.This is
One of innovative point of the present invention.2. as how formed two dimensional image：Nonlinear optics imaging needs to use inner scanning or outside
Scan to form two dimensional image, and can not be using the method for whole audience imaging.Inner scanning is to use piezoelectricity in the distal end of endoscope
Microdrive driving simple optical fiber vibration carries out two-dimensional scan or realizes two-dimensional scan using MEMS microdrive.It is internal
The benefit of scanning is can to use the transmission ultrashort laser pulse of single photonic crystal fiber, and shortcoming is either to use piezoelectricity micro-move device
Device or MEMS microdrive, the volume of the endoscope assembled is excessive, and particularly distal end rigid element is long, can not
Pass through the physiological bending portion of human body alimentary canal and the bendable portion of commercial electronic gastroscope.External scan is by the scanning of outside
Device, which is responsible for ultrashort laser pulse being coupled into the two dimension such as imaging optical fiber bundle, passes optical element, so as to by exciting light from being distally transferred to
Sample, then the image of sample is passed back to distal end.The advantages of external scan is convenient and commercial microscopes, without taking in addition
Build imaging system.Its shortcoming is imaging optical fiber bundle to the serious broadening of ultrashort laser pulse, is not used to nonlinear optics imaging.This
Invention employs external scan and is combined with lens relay.3. how high the various non linear optical signals of transmitting are in sample
Efficient collect：The excitation wavelength of two-photon excitation autofluorescence is 800nm or so, and launch wavelength is 516nm or so.It is secondary humorous
The excitation wavelength that ripple occurs is 800nm or so, and launch wavelength is 400nm or so.Triple-frequency harmonics occur excitation wavelength be
1200nm to 1550nm or so, launch wavelength are 400nm to 500nm or so.Coherent anti-stokes raman scattering is four photons
The Third-order nonlinearity of participation is, it is necessary to pump wavelength is 817nm, the stokes light caused by optical parametric amplifier
Wavelength is 1064nm, and the wavelength of anti-Stokes light is 663nm.It can be seen that nonlinear optics imaging in, excitation wavelength with it is non-
Linear effect wavelength differs greatly, and this is that high s/n ratio detection is brought conveniently, but the design proposition to optical system is very high
Requirement because obvious aberration can be produced, cause optical system detection efficiency and image quality to substantially reduce.Therefore detect single
The optical system of one nonlinear effect must be achromatic, and the optical system for detecting a variety of nonlinear effects must be disappeared again
Aberration, and application is peeped in being limited to, the diameter and length to optical system have considerable restraint, design, processing and assembling hardly possible
Degree is very high.Present invention employs multi-disc micro lens relay imaging.Single-sheet miniature lens have that diameter is small, and profile easy processing etc. is excellent
Point, but aberration is larger, numerical aperture is relatively low.Therefore this project employs micro lens relay transmission ultrashort pulse,
Non linear optical signal is not transmitted, and employs achromat-design, realizes the simplification of optical system.
In summary, scanning imaging system is peeped in presently, there are, mainly there are two classes：One kind is to use piezo-electric micro driver
Or MEMS microdrive carries out the non-linear optical endoscope of inner scanning, mainly realize that scan forward is imaged, still
Distal end rigid element is oversize, cannot be introduced into the alimentary canal of people.Another kind of is the copolymerization for being carried out using imaging optical fiber bundle external scan
Burnt endoscope, the alimentary canal of people can be entered, but because imaging optical fiber bundle is serious to ultra-short pulse laser broadening, can not excited non-
Linear effect.
The present invention proposes the multi-mode nonlinear optics with minor diameter flexible imaging transmission light path and scans based endoscopic imaging
System uses, and can be combined with electronic gastroscope and realize the noninvasive biopsy of early carcinoma of stomach.
The content of the invention
It is real especially with the miniature relay lens of multi-disc the present invention relates to a kind of nonlinear optics flexible endoscope imaging device
Existing minor diameter flexible imaging light path, the distal end of the light path have miniature apochromatic objective, can be by outside two-dimensional scan
The instrument channel for the commercial endoscope that multi-wavelength's ultrashort pulse can be bent by distal end with small-bend radius focuses to
Human internal organs, so as to inspire the various non linear optical signals with diagnostic significance, and will be various non-thread by optical fiber
Property optical signalling is transmitted to external nonlinear optics detection device.The present invention can be used for the clinical noninvasive inspection for realizing early-stage cancer
Survey.
To achieve the above object, the present invention is using technical scheme：It includes one group of coupled lens, multigroup relay lens,
A piece of dichroscope, a piece of speculum, one group of fiber coupling lens, one group of object lens and an optical fiber.The input of coupled lens
It is connected with elements such as outside two-dimensional scanner and its attached lenses；The output end of coupled lens and the input of first group of relay lens
End is connected；The output end of first group of relay lens is connected with the input of second group of relay lens；By that analogy, in N-1 groups
Output end after lens is connected with the input of N group relay lens；The output end of N group relay lens and dichroscope it is defeated
Enter end to be connected；One output end of dichroscope is connected with the input of object lens；The output end of object lens is connected with external sample；Two
It is connected to another output end of Look mirror with the input of speculum；The output end of speculum and the input of fiber coupling lens
It is connected；The output end of fiber coupling lens is connected with the input of optical fiber；The output end of optical fiber and outside photoelectric detector and its
The elements such as attached lenses, dichroscope, optical filter are connected；The elements such as outside two-dimensional scanner and its attached lenses are by two-dimensional scan
Ultrafast laser import first group of relay lens by coupled lens, first group of relay lens is by two-dimensional scan planar imaging the
In the air gap between one group of relay lens and second group of relay lens, second group of relay lens continues two-dimensional scan plane
It is imaged in the air gap between second group of relay lens and the 3rd group of relay lens, by that analogy, N groups relay lens will
Two-dimensional scan plane continues in the air gap that is imaged between N groups relay lens and dichroscope, and ultrafast laser passes through two
Focus in external sample to Look mirror and by objective scan, collected in the non linear optical signal that focal point inspires by object lens,
90 degree are reflected after dichroscope reflects 90 degree, then by speculum, optical fiber is coupled into by fiber coupling lens and is finally transmitted
To elements such as outside photoelectric detector and its attached lenses, dichroscope, optical filters.
Described coupled lens are achromat-design, and design wavelength is 600nm to any 2 wavelength between 1700nm,
Usually 817nm and 1064nm, but the two wavelength are not limited to, material is optical glass or high molecular polymer, and there is increasing on surface
The optical coating of strong transmissivity, for by the ultrafast laser of the two-dimensional scan of the elements such as outside two-dimensional scanner and its attached lenses
Import multigroup relay lens；
Described relay lens is achromat-design, and design wavelength is 600nm to any 2 wavelength between 1700nm, generally
For 817nm and 1064nm, but the two wavelength are not limited to, material is optical glass or high molecular polymer, and surface has enhancing saturating
The optical coating of rate is penetrated, the two-dimensional scan plane for ultrafast laser carries out relay imaging, has sky between multigroup relay lens
Gas gap, the two-dimensional scan plane of ultrafast laser are focused in the air gap, and two adjacent groups relay lens passes through rotatable
Mechanical structure is connected；
Described dichroscope is cube structure, and material is optical glass or high molecular polymer, and there is enhancing transmissivity on surface
Optical coating, inside has the optical thin film in 45 degree of angles with incident light axis, for transmiting near infrared band（700nm-
1300nm）Ultrafast laser and reflect visible light wave range（350nm-700nm）Non linear optical signal；
Described object lens are achromat-design, and design wavelength is 600nm to any 2 wavelength between 1700nm, is usually
817nm and 1064nm, but the two wavelength are not limited to, material is optical glass or high molecular polymer, and surface has enhancing to transmit
The optical coating of rate, for the ultrafast laser of incidence to be focused in external sample, non linear optical signal is inspired, and to fall
The mode for penetrating formula detection collects non linear optical signal；
The material of described speculum is optical glass or high molecular polymer, and there is the optical coating of enhancing reflectivity on surface, if
Meter wavelength is 350nm to 700nm, for will be seen that the non linear optical signal of optical band reflects 90 degree；
Described fiber coupling lens are achromat-design, and design wavelength is 350nm to any 2 wavelength between 700nm, is led to
Often it is 400nm and 633nm, but is not limited to the two wavelength, material is optical glass or high molecular polymer, and surface has enhancing saturating
The optical coating of rate is penetrated, the non linear optical signal for will be seen that optical band focuses on and is coupled into optical fiber；
Described optical fiber is multimode fibre, and transmission wavelength is 350nm to 700nm.
The operation principle of the present invention is such：In order to realize TPEAF, SHG and CARS detection, outside ultrafast laser light
Source needs to provide 817nm femtosecond laser and 1064nm picosecond laser.The two is accurate coaxial conllinear, wherein 817nm femtoseconds
Laser is used for TPEAF, SHG exciting light, produces 500nm TPEAF and 400nm SHG signals, also serves as CARS pumping
Light, produce 633nm CARS signals；1064nm picosecond laser is used as CARS stokes light.Femtosecond comprising 817nm swashs
The ultrashort pulse of light and 1064nm picosecond laser（Hereinafter referred to as ultrashort pulse）By outside two-dimensional scanner
Two-dimensional scan is carried out, passes through scanning lens, a series of scanning optical paths such as sleeve lens and micro objective, through overcoupling afterwards
Object lens enter the present invention.It is the achromat-design for 817nm and 1064nm wavelength to couple object lens.Multigroup relay lens be for
The achromat-design of 817nm and 1064nm wavelength, the two-dimensional scan plane for ultrafast laser carry out relay imaging, multigroup relaying
There is the air gap, the two-dimensional scan plane of ultrafast laser is focused in the air gap, and avoiding to compensate between lens
The ultrashort pulse broadening that brings of self phase modulation, and the group delay dispersion brought by medium in excitation light path can be by
Outside pulse shortener carries out precommpression to compensate to ultrashort pulse.Two adjacent groups relay lens passes through rotatable machinery
Structure is connected, it is achieved thereby that the bending transmission of two-dimensional scan ultrafast laser.N groups relay lens by two-dimensional scan plane after
Continuous to be imaged in the air gap between N groups relay lens and dichroscope, ultrafast laser passes through dichroscope（Transmission
700nm-1300nm；Reflect 350nm-700nm）And focused on by objective scan in external sample, inspired in focal point
TPEAF, SHG, CARS non linear optical signal are collected by object lens again.Object lens are the achromatism for 817nm and 1064nm wavelength
Design, it means that for object lens for 400nm SHG, 500nm TPEAF and 633nm CARS signals have larger aberration,
And this part aberration will carry out part compensation by fiber coupling lens.Non linear optical signal reflects 90 degree by dichroscope
Afterwards, then by speculum reflect 90 degree, be coupled into optical fiber by fiber coupling lens and finally transmit to outside photoelectric detector and its
The elements such as attached lenses, dichroscope, optical filter.Fiber coupling lens set for the achromatism for 400nm and 633nm wavelength
Meter, although there is certain aberration for 500nm wavelength, because wavelength is at a distance of nearer, it should influence little.The present invention realizes
Excitation light path and the separate design for collecting light path, it is big to avoid volume, baroque multi-wavelength apochromatic lens design,
And 817nm and 1064nm is directed in excitation light path, and small volume is used for 400nm and 633nm collecting light path, it is simple in construction
Achromatic lens designs.Optical fiber is major diameter multimode fibre.Outside photoelectric detector is multiple photomultipliers or the pole of snowslide two
Pipe.Non linear optical signal is by dichroscope outside multi-disc by the Signal separator of different wave length out by different photomultiplier transits
Pipe or avalanche diode are detected.
The present invention is by adopting the above-described technical solution, have the following advantages that：
1st, the bending of two-dimensional scan ultrafast laser is realized using the multigroup relay lens connected by rotatable mechanical structure
Transmission；
2nd, excitation light path and collect the separate design of light path and avoid that volume is big, and baroque multi-wavelength apochromatic lens is set
Meter, and 817nm and 1064nm is directed in excitation light path, use small volume, structure letter for 400nm and 633nm collecting light path
Single achromatic lens design；
3rd, by the way that the focus of two-dimensional scan plane is placed in the air gap between multigroup relay lens, avoiding to compensate
The ultrashort pulse broadening that brings of self phase modulation；And the group delay dispersion brought by medium in excitation light path can be by
Outside pulse shortener carries out precommpression to compensate to ultrashort pulse；
4th, simple in construction, small volume, cost is low, can be used in combination with commercial gastroscope, can be a variety of to TPEAF, SHG, CARS etc.
Nonlinear properties detect simultaneously, realize and realize live body, functional imaging in situ, are expected to realize the inspection of early-stage cancer
Survey.
Brief description of the drawings
Fig. 1 is the structured flowchart of the present invention；
Fig. 2 is the connection diagram with external optical imaging device of the present invention；
Fig. 3 is the geometric optics simulation result that multigroup relay lens bends optical axis.
Embodiment
The invention will be further described with reference to the accompanying drawings and examples：As Figure 1-3, it is saturating to include one group of coupling for it
Mirror 1, multigroup relay lens 2, a piece of dichroscope 3, a piece of speculum 5, one group of fiber coupling lens 6, one group of object lens 4 and one
Optical fiber 7.The input of coupled lens 1 is connected with elements such as outside two-dimensional scanner and its attached lenses；The output of coupled lens 1
End is connected with the input of first group of relay lens 2.1；The output end of first group of relay lens 2.1 and second group of relay lens
2.2 input is connected；By that analogy, N-1 groups relay lens 2.N-1 output end and N group relay lens 2.N input
End is connected；N group relay lens 2.N output end is connected with the input of dichroscope 3；One output end of dichroscope 3
It is connected with the input of object lens 4；The output end of object lens 4 is connected with external sample；Another output end of dichroscope 3 and reflection
The input of mirror 5 is connected；The output end of speculum 5 is connected with the input of fiber coupling lens 6；Fiber coupling lens 6 it is defeated
Go out end with the input of optical fiber 7 to be connected；The output end of optical fiber 7 and outside photoelectric detector and its attached lenses, dichroscope, filter
The elements such as mating plate are connected；The elements such as outside two-dimensional scanner and its attached lenses are saturating by coupling by the ultrafast laser of two-dimensional scan
Mirror 1 enters first group of relay lens 2.1, and first group of relay lens 2.1 is by two-dimensional scan planar imaging in the first set after lens 2.1
In the air gap between second group of relay lens 2.2, two-dimensional scan plane is continued to be imaged on by second group of relay lens 2.2
In the air gap between second group of relay lens 2.2 and the 3rd group of relay lens 2.3, by that analogy, N group relay lens
2.N continues two-dimensional scan plane in the air gap that is imaged between N group relay lens 2.N and dichroscope 3, ultrafast
Laser is by dichroscope 3 and scanning focused in external sample by object lens 4, in the non linear optical signal that focal point inspires
Collected by object lens 4, reflect 90 degree after dichroscope 3 reflects 90 degree, then by speculum 5, be coupled into by fiber coupling lens 6
Optical fiber 7 is simultaneously finally transmitted to elements such as outside photoelectric detector and its attached lenses, dichroscope, optical filters.
Described coupled lens 1 are achromat-design, and design wavelength is 600nm to any 2 wavelength between 1700nm,
Usually 817nm and 1064nm, but the two wavelength are not limited to, material is optical glass or high molecular polymer, and there is increasing on surface
The optical coating of strong transmissivity, for by the ultrafast laser of the two-dimensional scan of the elements such as outside two-dimensional scanner and its attached lenses
Import multigroup relay lens 2；
Described relay lens 2 is achromat-design, and design wavelength is 600nm to any 2 wavelength between 1700nm, generally
For 817nm and 1064nm, but the two wavelength are not limited to, material is optical glass or high molecular polymer, and surface has enhancing saturating
The optical coating of rate is penetrated, the two-dimensional scan plane for ultrafast laser carries out relay imaging, has sky between multigroup relay lens 2
Gas gap, the two-dimensional scan plane of ultrafast laser are focused in the air gap, and two adjacent groups relay lens 2 is by rotatable
Mechanical structure is connected；
Described dichroscope 3 is cube structure, and material is optical glass or high molecular polymer, and there is enhancing transmissivity on surface
Optical coating, inside has the optical thin film in 45 degree of angles with incident light axis, for transmiting the ultrafast of 600nm-1700nm
Laser simultaneously reflects visible light wave range（350nm-700nm）Non linear optical signal；
Described object lens 4 are achromat-design, and design wavelength is 600nm to any 2 wavelength between 1700nm, is usually
817nm and 1064nm, but the two wavelength are not limited to, material is optical glass or high molecular polymer, and surface has enhancing to transmit
The optical coating of rate, for the ultrafast laser of incidence to be focused in external sample, non linear optical signal is inspired, and to fall
The mode for penetrating formula detection collects non linear optical signal；
The material of described speculum 5 is optical glass or high molecular polymer, and there is the optical coating of enhancing reflectivity on surface, if
Meter wavelength is 350nm to 700nm, for will be seen that the non linear optical signal of optical band reflects 90 degree；
Described fiber coupling lens 6 are achromat-design, and design wavelength is 350nm to any 2 wavelength between 700nm,
Usually 400nm and 633nm, but the two wavelength are not limited to, material is optical glass or high molecular polymer, and there is enhancing on surface
The optical coating of transmissivity, the non linear optical signal for will be seen that optical band focus on and are coupled into optical fiber 7；
The described multimode fibre of optical fiber 7, transmission wavelength are 350nm to 700nm.
The operation principle of the present invention is such：Illustrated with realizing exemplified by TPEAF, SHG and CARS detection, it is outside ultrafast
LASER Light Source needs to provide 817nm femtosecond laser and 1064nm picosecond laser.The two is accurate coaxial conllinear, wherein
817nm femtosecond lasers are used for TPEAF, SHG exciting light, produce 500nm TPEAF and 400nm SHG signals, also serve as
CARS pump light, produce 633nm CARS signals；1064nm picosecond laser is used as CARS stokes light.Comprising
The ultrashort pulse of 817nm femtosecond laser and 1064nm picosecond laser（Hereinafter referred to as ultrashort pulse）By outer
Portion's two-dimensional scanner carries out two-dimensional scan, passes through scanning lens, a series of scanning light such as sleeve lens and micro objective afterwards
Road, entering the present invention by coupling object lens 1.It is the achromat-design for 817nm and 1064nm wavelength to couple object lens 1.It is more
Group relay lens 2 is the achromat-design for 817nm and 1064nm wavelength, and the two-dimensional scan plane for ultrafast laser is carried out
Relay imaging, there is the air gap between multigroup relay lens 2, the two-dimensional scan plane of ultrafast laser is focused on the air gap
In, ultrashort pulse broadening that self phase modulation that avoiding to compensate is brought, and by medium belt in excitation light path
The group delay dispersion come can carry out precommpression to compensate by outside pulse shortener to ultrashort pulse.Two adjacent groups relaying is saturating
Mirror 2 is connected by rotatable mechanical structure, it is achieved thereby that the bending transmission of two-dimensional scan ultrafast laser.N groups relay
Lens 2.N continues two-dimensional scan plane in the air gap that is imaged between N group relay lens 2.N and dichroscope 3,
Ultrafast laser passes through dichroscope 3（Transmit 600nm-1700nm；Reflect 350nm-700nm）It is and scanning focused outside by object lens 4
In portion's sample, in the TPEAF that focal point inspires, SHG, CARS non linear optical signals are collected by object lens 4 again.Object lens 4 are pin
To the achromat-design of 817nm and 1064nm wavelength, it means that object lens 4 for 400nm SHG, 500nm TPEAF and
633nm CARS signals have larger aberration, and this part aberration will carry out part compensation by fiber coupling lens 6.It is non-
Linear optics signal reflects 90 degree after dichroscope 3 reflects 90 degree, then by speculum 5, is coupled into by fiber coupling lens 6
Optical fiber 7 is simultaneously finally transmitted to elements such as outside photoelectric detector and its attached lenses, dichroscope, optical filters.Fiber coupling is saturating
Mirror 6 is the achromat-design for 400nm and 633nm wavelength, although there is certain aberration for 500nm wavelength, due to
Wavelength is at a distance of nearer, it should influences little.The present invention realizes excitation light path with collecting the separate design of light path, avoids volume
Greatly, baroque multi-wavelength apochromatic lens design, and 817nm and 1064nm is directed in excitation light path, collecting light path
Small volume, achromatic lens simple in construction design are used for 400nm and 633nm.Optical fiber is major diameter multimode fibre.It is outside
Photoelectric detector is multiple photomultipliers or avalanche diode.Non linear optical signal will not by dichroscope outside multi-disc
The Signal separator of co-wavelength is out detected by different photomultiplier or avalanche diode.
Claims (8)
- A kind of 1. nonlinear optics flexible endoscope imaging device, it is characterised in that：It includes one group of coupled lens, and multigroup relaying is saturating Mirror, a piece of dichroscope, a piece of speculum, one group of fiber coupling lens, one group of object lens and an optical fiber；The input of coupled lens End is connected with elements such as outside two-dimensional scanner and its attached lenses；The output end of coupled lens is defeated with first group of relay lens Enter end to be connected；The output end of first group of relay lens is connected with the input of second group of relay lens；By that analogy, N-1 groups The output end of relay lens is connected with the input of N group relay lens；The output end of N group relay lens and dichroscope Input is connected；One output end of dichroscope is connected with the input of object lens；The output end of object lens is connected with external sample； Another output end of dichroscope is connected with the input of speculum；The output end of speculum and the input of fiber coupling lens End is connected；The output end of fiber coupling lens is connected with the input of optical fiber；The output end of optical fiber and outside photoelectric detector and The elements such as its attached lenses, dichroscope, optical filter are connected；The elements such as outside two-dimensional scanner and its attached lenses sweep two dimension The ultrafast laser retouched imports first group of relay lens by coupled lens, and first group of relay lens exists two-dimensional scan planar imaging In the air gap between first group of relay lens and second group of relay lens, second group of relay lens by two-dimensional scan plane after It is continuous to be imaged in the air gap between second group of relay lens and the 3rd group of relay lens, by that analogy, N group relay lens Two-dimensional scan plane is continued in the air gap that is imaged between N groups relay lens and dichroscope, ultrafast laser passes through Dichroscope is simultaneously focused in external sample by objective scan, is received in the non linear optical signal that focal point inspires by object lens Collection, 90 degree are reflected after dichroscope reflects 90 degree, then by speculum, optical fiber is coupled into by fiber coupling lens and is finally passed Transport to the elements such as outside photoelectric detector and its attached lenses, dichroscope, optical filter.
- A kind of 2. nonlinear optics flexible endoscope imaging device as claimed in claim 1, it is characterised in that：Described coupling is saturating Mirror is achromat-design, and design wavelength is 600nm to any 2 wavelength between 1700nm, usually 817nm and 1064nm, But the two wavelength are not limited to, material is optical glass or high molecular polymer, and there is the optical coating of enhancing transmissivity on surface.
- A kind of 3. nonlinear optics flexible endoscope imaging device as claimed in claim 1, it is characterised in that：Described relaying is saturating Mirror is achromat-design, and design wavelength is 600nm to any 2 wavelength between 1700nm, usually 817nm and 1064nm, But the two wavelength are not limited to, material is optical glass or high molecular polymer, and there is the optical coating of enhancing transmissivity on surface, uses Relay imaging is carried out in the two-dimensional scan plane of ultrafast laser, there is the air gap between multigroup relay lens, ultrafast laser Two-dimensional scan plane is focused in the air gap, and adjacent two groups of relay lens are connected by rotatable mechanical structure.
- A kind of 4. nonlinear optics flexible endoscope imaging device as claimed in claim 1, it is characterised in that：Described dichroic Mirror is cube structure, and material is optical glass or high molecular polymer, and there are the optical coating of enhancing transmissivity, inside tool in surface There is the optical thin film in 45 degree of angles with incident light axis.
- A kind of 5. nonlinear optics flexible endoscope imaging device as claimed in claim 1, it is characterised in that：Described object lens are Achromat-design, design wavelength are 600nm to any 2 wavelength between 1700nm, usually 817nm and 1064nm, but not It is limited to the two wavelength, material is optical glass or high molecular polymer, and there is the optical coating of enhancing transmissivity on surface, for inciting somebody to action Incident ultrafast laser is focused in external sample, inspires non linear optical signal, and collected in a manner of falling to penetrate formula detection Non linear optical signal.
- A kind of 6. nonlinear optics flexible endoscope imaging device as claimed in claim 1, it is characterised in that：Described speculum Material be optical glass or high molecular polymer, there is an optical coating of enhancing reflectivity on surface, design wavelength be 350nm extremely 700nm。
- A kind of 7. nonlinear optics flexible endoscope imaging device as claimed in claim 1, it is characterised in that：Described optical fiber coupling Conjunction lens are achromat-design, and design wavelength is 350nm to any 2 wavelength between 700nm, usually 400nm and 633nm, but the two wavelength are not limited to, material is optical glass or high molecular polymer, and there is the optics of enhancing transmissivity on surface Plated film, the non linear optical signal for will be seen that optical band focus on and are coupled into optical fiber.
- A kind of 8. nonlinear optics flexible endoscope imaging device as claimed in claim 1, it is characterised in that：Described optical fiber is Multimode fibre, transmission wavelength are 350nm to 700nm.
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