CN109730626A - Cavity endoscope detection device and three dimensional non-linear laser scanning cavity endoscope - Google Patents
Cavity endoscope detection device and three dimensional non-linear laser scanning cavity endoscope Download PDFInfo
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Abstract
The embodiment of the present invention provides a kind of cavity endoscope detection device and three dimensional non-linear laser scanning cavity endoscope.Wherein, above-mentioned cavity endoscope detection device includes handle housing and detection pipes, relay lens and object lens are provided in the detection channels of detection pipes, and the first optical path of formation and the second optical path, the first optical path include collimation lens, micro electromechanical scanning galvanometer, lens, dichroscope, relay lens and object lens；Second optical path includes object lens, relay lens and dichroscope.Cavity endoscope detection device provided in an embodiment of the present invention and three dimensional non-linear laser scanning cavity endoscope, which are used, is arranged the first optical path and the second optical path in the inner space of handle housing and detection pipes, realize the acquisition to the excitation of autofluorescence substance and two-photon and second harmonic signal, wherein, the use of relay lens is so that detection pipes can be extended as needed, it is easy to operate, easy to use to meet the detection needs to stomach intestinal tissue and oral cavity tissue etc..
Description
Technical field
The present embodiments relate to laser scanning endoscopic technique field more particularly to a kind of cavity endoscope detection devices
And three dimensional non-linear laser scanning cavity endoscope.
Background technique
Gastrointestinal cancer is to induce the second largest reason of developed country crowd cancer stricken death, and in recent years should
Trend is more and more obvious.Surgery radical operation is mainly used for the treatment of gastrointestinal cancer, but is embodied outer
When section's radical operation it needs to be determined that the operation excision specific range, therefore carry out operation consent, it is to be understood that tumour
Whether there is or not Cancer residuals etc. for good pernicious, invasive depth, transfer case and incisxal edge.Therefore biopsy is swollen for gastrointestinal tract under preoperative Gastrointestinal Endoscopes
Tumor tissue diagnosis is a critically important diagnostic evidence.And according to tumorous size, growth position, invasive depth etc., by gastric cancer
Art formula be divided into that stomach is cut entirely, stomach time is cut entirely, gastric resection and endoscopic inferior mucosa or submucous resection etc..
And at present, biopsy carries out iconography imaging usually based on Gastrointestinal Endoscopes supplemented by CT, MRI etc. under Gastrointestinal Endoscopes, or
Assessment is carried out to many gastrointestinal diseases with traditional white light laparoscope or endoscope to deposit.
But based on Gastrointestinal Endoscopes, the imaging of progress iconography haves the shortcomings that some inevitable supplemented by CT, MRI etc.,
For example be easy to cause intestinal tube or knurl bleeding in operation, need artificial drawing or squeeze, when Gastrointestinal Endoscopes cannot pass through intestinal tube,
Endoscopic biopsy is carried out repeatedly so that time delay, if causing severe haemorrhage also needs additional first aid hemostasis etc..And CT, MRI etc.
Complementary detection methods are unable to judge accurately the invasive depth and lymphatic metastasis of upper gastrointestinal road tumour in clinical practice
Situation.And gastroenteric tumor T is judged by stages by endoscopic ultrasonography, its accuracy of document report is only 44.7%~78%, insufficient
To become a reliable diagnostic criteria.Endoscopic ultrasonography is also ineffective to the preoperative judge of local resection operation, can not be accurate
Gastrointestinal mucosa level is segmented, and effect is also poor by stages to N.Therefore, just it is badly in need of one from the point of view of current gastrointestinal tract aided diagnosis technique
The new gastroenteric tumor diagnostic device of kind, with real-time detection stomach intestinal tissue in situ information.
Summary of the invention
For the technical problems in the prior art, the embodiment of the present invention provide a kind of cavity endoscope detection device and
Three dimensional non-linear laser scanning cavity endoscope.
In a first aspect, the embodiment of the present invention provides a kind of cavity endoscope detection device, comprising:
Handle housing and detection pipes, the handle housing are fixedly connected with the detection pipes, setting in the detection pipes
There are detection channels, relay lens and object lens are provided in the detection channels, the object lens are located at the channel of the detection channels
At mouthful, the light channel structure being arranged in the object lens, the relay lens and the handle housing forms the first optical path and the second optical path,
The axle center of the detection channels is overlapped with the detection pipes axle center, in which:
First optical path successively includes between the handle housing inner fiber general-purpose interface and the passway
Collimation lens, micro electromechanical scanning galvanometer, lens, dichroscope, the relay lens and the object lens, wherein first optical path
The passway is interfaced to from the fiber optic universal for conducting the received laser signal of the collimation lens；
Second optical path successively includes object lens between the passway and the fiber optic universal interface, institute
Relay lens and the dichroscope are stated, wherein second optical path is for conducting the collected optical signal of the object lens from described
Passway is to the fiber optic universal interface.
Second aspect, the embodiment of the present invention provide a kind of three dimensional non-linear laser scanning cavity endoscope, comprising:
Phosphor collection device, scanning collection controller, femtosecond pulse laser, fiber coupling module and the present invention are real
Apply the cavity endoscope detection device that first aspect provides, the phosphor collection device and the fiber coupling module are and institute
State cavity endoscope detection device fiber optic communication connection, the phosphor collection device and the cavity endoscope detection device with
The scanning collection controller electrical connection, in which:
The femtosecond pulse laser, for exporting pulsed laser signal to the fiber coupling module；
The fiber coupling module, for coupling the pulsed laser signal of the femtosecond pulse laser output, and
Transmit collimation lens described in the pulsed laser signal to the cavity endoscope detection device；
The cavity endoscope detection device exports the pulse laser letter after receiving the pulsed laser signal
Number autofluorescence substance intracellular to life entity, and generated after obtaining the autofluorescence substance excitation by the object lens
Fluorescence signal and second harmonic signal, and export the fluorescence signal and the second harmonic signal to the phosphor collection and fill
It sets；
The phosphor collection device converts institute after receiving the fluorescence signal and the second harmonic signal respectively
It states fluorescence signal and the second harmonic signal is corresponding electric signal；
The scanning collection controller sweeps the pulsed laser signal for controlling the micro electromechanical scanning galvanometer
Retouch and synchronous acquisition described in electric signal.
Cavity endoscope detection device provided in an embodiment of the present invention is fixedly connected using handle housing and detection pipes
Constituted mode is arranged the first optical path and the second optical path in the inner space of two component parts, peeps to be formed in hand-held cavity
Mirror detection device, the first optical path include collimation lens, micro electromechanical scanning galvanometer, lens, dichroscope, the relay lens and institute
Object lens are stated, for conducting the laser signal of excitation autofluorescence substance；Second optical path includes object lens and the dichroscope, is used
In collection two-photon signal and second harmonic signal, wherein the use of relay lens is so that detection pipes can be prolonged as needed
It is long, it is easy to operate, easy to use to meet the detection needs to intraperitoneal gastrointestinal tissue, oral cavity tissue and uterine cavity inner tissue.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is this hair
Bright some embodiments for those of ordinary skill in the art without creative efforts, can be with root
Other attached drawings are obtained according to these attached drawings.
Fig. 1 is cavity endoscope detection device structural schematic diagram one provided in an embodiment of the present invention；
Fig. 2 is cavity endoscope detection device structural schematic diagram two provided in an embodiment of the present invention；
Fig. 3 is cavity endoscope detection device structural schematic diagram three provided in an embodiment of the present invention；
Fig. 4 is cavity endoscope detection device structural schematic diagram four provided in an embodiment of the present invention；
Fig. 5 is three dimensional non-linear laser scanning cavity endoscope structure schematic diagram one provided in an embodiment of the present invention；
Fig. 6 is three dimensional non-linear laser scanning cavity endoscope structure schematic diagram two provided in an embodiment of the present invention；
Fig. 7 is phosphor collection apparatus structure schematic diagram provided in an embodiment of the present invention；
Fig. 8 is the envelope of the box composite structure of three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention
Box structure schematic diagram one；
Fig. 9 is the envelope of the box composite structure of three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention
Box structure schematic diagram two；
Figure 10 provides the mesa structure schematic diagram one of three dimensional non-linear laser scanning cavity endoscope for the embodiment of the present invention；
The mesa structure schematic diagram two of Figure 11 embodiment of the present invention offer three dimensional non-linear laser scanning cavity endoscope.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art
Every other embodiment obtained without creative efforts, shall fall within the protection scope of the present invention.
At present based on Gastrointestinal Endoscopes, iconography imaging is carried out supplemented by CT, MRI etc. to obtain the good pernicious, leaching of tumour
Moisten depth, transfer case and incisxal edge whether there is or not relevant informations such as Cancer residuals, have the shortcomings that in concrete operations, for example is easy
Lead to intestinal tube or knurl bleeding, need artificial drawing or squeeze, when Gastrointestinal Endoscopes cannot pass through intestinal tube, carries out endoscopic biopsy repeatedly
So that time delay, if causing severe haemorrhage also needs additional first aid hemostasis etc..And the complementary detection methods such as CT, MRI,
The invasive depth and lymphatic metastasis situation of upper gastrointestinal road tumour are unable to judge accurately in clinical practice.And by ultrasound
Mirror judges gastroenteric tumor T by stages, its accuracy of document report is only 44.7%~78%, and being not enough to, which becomes one, reliably examines
Disconnected standard, and endoscopic ultrasonography is ineffective to the preoperative judge of local resection operation, can not precisely subdivided gastrointestinal mucosa level, and
To N, effect is also poor by stages.
And traditional white light laparoscope can assess many gastrointestinal diseases with endoscope, but the technology is only limitted to examine
Survey general form variation.Although being easy to find suspicious region, compared with In vivo detection technology, these technologies and false positive rate
And specificity etc. is related.White light endoscopy is associated with the extensive error that micro-variations diagnose, including ulcerative colitis
Or the inspection diagnosis including Barrett oesophagus and Flat Adenoma depauperation.Confocal endoscope combination laser technology, fluorescence are visited
Survey technology, fast scanning techniques etc. are because that can detect mucous membrane variation in microscopic scale, it is possible to for replacing tissue biopsy, and by
To extensive concern, which has highly sensitive and specificity.But be copolymerized burnt based endoscopic imaging technology still by
As the limitation of depth and fluorescent dye, since stomach and intestine sample has very strong absorption and scattering, imaging depth only to exist visible light
Superficial layer, and it also requires injecting specific fluorescent staining developer, operation is excessively complicated, cannot accurately obtain the leaching of tumour
Moistening depth, transfer case and surgical operation incisxal edge, whether there is or not the relevant informations such as Cancer residual.
And two-photon micro-imaging technique uses the longer femtosecond pulse laser of wavelength to have imaging as excitation light source
The features such as depth is deep, light injury is small, photobleaching region is small, phosphor collection is high-efficient, has in the imaging deep to biological tissue
There is epoch-making meaning.It is micro- that the W.Denk et al. of nineteen ninety Cornell University has developed First two-photon fluorescence in the world
Mirror, using the multi-photon micro-imaging technique based on nonlinear optics and femtosecond pulse.The technology is by utilizing living body
The second harmonic that the autofluorescence and collagen tissue that cell itself generates in tissue generate, can obtain sample real-time, quickly
Institutional framework and cellular morphology.Early in 1986, second harmonic was used for skin research and the research of coronary artery micro-imaging, card
Its real feasibility that be used to observe biological tissue.MPM also can be used as an important tool of cancer research.Cell itself produces
Raw autofluorescence from intracellular nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) (FAD),
It is 460nm that NADH, which issues wavelength, and the secondary oscillation harmonic wave of collagen is 370~390nm, so leading to when observing tumor specimen tissue
Often select the multiphoton microscope of 780~940nm range.MPM imaging is not only suitable with the tumor tissue pathology of standard, simultaneously
The additional information of tumor neogenetic process is also provided, such as can reflect the metabolism of tumor tissue cell by surveying rate value NADH/FAD
It is horizontal.
Using multi-photon imaging technique, multiphoton microscope is capable of providing real-time stomach intestinal tissue's structure and cellular morphology
Learn information.Multi-photon imaging technique has without exogenous marker tissue, extremely sensitive to collagen, small to the light injury of tissue and wear
The features such as depth is deep thoroughly, may be applied to the optical biopsy of gastroenteric tumor.There is presently no useful clinically two-photon abdomens
Hysteroscope and endoscope, and the cavity endoscope detection device based on two photon imaging, with real-time detection stomach intestinal tissue in situ
Information.
For real-time detection stomach intestinal tissue in situ information, the embodiment of the invention provides a kind of detections of cavity endoscope to fill
It sets, Fig. 1 is cavity endoscope detection device structural schematic diagram one provided in an embodiment of the present invention, as shown in Figure 1, peeping in the cavity
Mirror detection device includes:
Handle housing 11 and detection pipes 12, handle housing 11 are fixedly connected with detection pipes 12, are provided in detection pipes 12
Detection channels are provided with relay lens 122 and object lens 121 in detection channels, and object lens 121 are located at the passway of detection channels,
The light channel structure being arranged in object lens 121, relay lens 122 and handle housing 11 forms the first optical path and the second optical path, detection channels
Axle center be overlapped with 12 axle center of detection pipes, in which:
First optical path successively includes the collimation lens between 11 inner fiber general-purpose interface 111 of handle housing and passway
112, micro electromechanical scanning galvanometer 114, lens 115, dichroscope 116, relay lens 122 and object lens 121, wherein the first optical path is used
In the conduction received laser signal of collimation lens 112 from fiber optic universal interface 111 to passway；
Second optical path successively include object lens 121 between passway and fiber optic universal interface 111, relay lens 122 with
And dichroscope 116, wherein the second optical path is for conducting the collected optical signal of object lens 121 from passway to fiber optic universal interface
111。
Specifically, cavity endoscope detection device provided in an embodiment of the present invention is integrated with two optical paths, and one is to be used for
The first optical path of laser signal is conducted, which is mainly used for exciting autofluorescence substance；The other is collecting and passing
Lead the two-photon signal and second harmonic signal generated after autofluorescence substance is excited；Wherein, the portion in this two optical paths
Being divided line structure includes collimation lens 112, micro electromechanical scanning galvanometer 114, lens 115 and dichroscope 116, is integrated in handle case
In body 11, and relay lens 122 and object lens 121 are integrated in detection pipes 12, and object lens 121 are arranged at the passway of detection channels,
Relay lens 122 is set in detection channels in 121 inside of object lens, and relay lens 122 is collected for conduction object lens 121 over long distances
Two-photon signal and second harmonic signal to dichroscope 116, the image planes of object lens 121 are overlapped with the focal plane of relay lens 122,
The effect of relay lens is that will pass through the laser signal scanning area of micro electromechanical scanning mirror for passing picture with the ratio etc. of 1:1 first
At conduction to the image planes of object lens 121.Secondly in the light signal transduction to handle housing that object lens 121 can be also collected by relay lens
Light channel structure part, be collected via the fiber optic bundle of light channel structure, wherein dichroscope 116 can for it is long lead to it is short by anti-two
It is transmitted spontaneous glimmering for exciting when that is, setting length leads to short anti-dichroscope 116 to Look mirror 116 or short elongated anti-dichroscope 116
The pulsed laser signal of stimulative substance, the two-photon signal and second harmonic signal that reflecting and collecting arrives, as shown in Figure 1, at this point, should
Cavity endoscope detection device can be the detection device of laparoscope；Fig. 2 is cavity endoscope provided in an embodiment of the present invention detection
Apparatus structure schematic diagram two, as shown in Fig. 2, being reflected spontaneous for exciting when the dichroscope is short elongated anti-dichroscope
The pulsed laser signal of fluorescent material transmits the two-photon signal and second harmonic signal being collected into, dichroscope reflection
From fiber optic universal interface 111 be emitted be incident on after collimation lens 112, micro electromechanical scanning galvanometer 114, lens 115 two to
Laser signal in Look mirror 116 transmits the collected two-photon signal of object lens 121 and second harmonic signal to object lens 121, this
When, which can be the detection device of mouth mirror, and wherein the detection device of the mouth mirror also includes handle
12 two parts of shell 11 and detection pipes.
Cavity endoscope detection device provided in an embodiment of the present invention is fixedly connected using handle housing and detection pipes
Constituted mode is arranged the first optical path and the second optical path in the inner space of two component parts, peeps to be formed in hand-held cavity
Mirror detection device, the first optical path include collimation lens, liquid lens, micro electromechanical scanning galvanometer, lens, dichroscope, relay lens
And object lens, for conducting the laser signal of excitation autofluorescence substance；Second optical path includes object lens and dichroscope, is used for
Collect two-photon signal and second harmonic signal, wherein the use of relay lens so that detection pipes can be extended as needed,
It is easy to operate, easy to use to meet the detection needs to stomach intestinal tissue and oral cavity tissue.
Optical path knot on the basis of the various embodiments described above, in cavity endoscope detection device provided in an embodiment of the present invention
Structure further includes liquid lens, and Fig. 3 is cavity endoscope detection device structural schematic diagram three provided in an embodiment of the present invention, such as Fig. 3
Shown, liquid lens 113 is between collimation lens 112 and micro electromechanical scanning galvanometer 114, to form the first new optical path, newly
The first optical path successively include collimation lens 112 between fiber optic universal interface 111 and passway, it is liquid lens 113, micro-
Electromechanical scanning galvanometer 114, lens 115, dichroscope 116 and object lens 121.I.e. liquid lens 113 setting so that, can pass through
Applying voltage or current to liquid lens 113 makes 113 surface of liquid lens generate the bending accordingly arrived, and then collimation lens
The directional light of 112 outgoing generates different focal powers.Specific optical path are as follows: laser signal is from fiber exit, by collimation lens 112
It is incident on liquid lens 113 in parallel afterwards, generates corresponding light focus from liquid lens 113 according to the voltage or current signal of load
Degree, outgoing are converging or diverging with light by micro electromechanical scanning galvanometer 114, lens 115, dichroscope 116, by relay lens 122
It is converged on sample after being transmitted to object lens 121.Wherein, the power variation that liquid lens 113 introduces can make 121 mouthfuls of object lens outgoing
The focus of laser signal be moved forward and backward in longitudinal direction, and the response speed of liquid lens 113 is very fast, scan frequency
In KHz magnitude, therefore the scanning imagery of quick longitudinal direction may be implemented.Wherein, liquid lens 113 do not apply voltage or
It is equivalent to parallel plate glass when current signal, without focal power and the focus after object lens 121 will not be made to generate laser signal
Any offset, to realize three-dimensional imaging.
Detection pipes on the basis of the various embodiments described above, in cavity endoscope detection device provided in an embodiment of the present invention
Several illumination channels are inside additionally provided with, Fig. 4 is cavity endoscope detection device structural schematic diagram four provided in an embodiment of the present invention,
As shown in figure 4, illumination channel 123 in be provided be used for transmission illumination optical signal illumination fiber optic bundle, wherein illumination channel 123 with
It is uniformly distributed centered on the axle center of detection channels.Detection pipes in cavity endoscope detection device i.e. provided in an embodiment of the present invention
Several illumination channels 123 are inside additionally provided with, which is both provided with illumination fiber optic bundle in each channel,
Lighting fiber has certain pore size angle, does not need lens and is used directly for divergent illumination, and illuminates channel 123 with detection channels
Axle center centered on be uniformly distributed, Uniform Illumination is provided for cavity endoscope detection device, before facilitating work viewing objective
Test serum zone state.
Detection pipes on the basis of the various embodiments described above, in cavity endoscope detection device provided in an embodiment of the present invention
It is inside additionally provided with observation channel, as shown in figure 4, observation channel is located between detection channels and illumination channel, in which:
It observes at the passway in channel and is provided with observation camera lens 124, the light field optical fiber in observation camera lens 124 and observation channel
Beam is connected, to obtain the test serum regional image information before object lens.Cavity endoscope i.e. provided in an embodiment of the present invention
Observation channel is additionally provided in detection pipes in detection device, which is located between detection channels and illumination channel, and
It is provided with observation camera lens 124 and light field fiber optic bundle, light field fiber optic bundle is imaging optical fiber bundle, caught for transmitting observation camera lens 124
Test serum regional image information before the object lens grasped, wherein observation channel can be one, or two formation are double
Three-dimensional light field cavity endoscope function is realized in visual observation.
Detection pipes on the basis of the various embodiments described above, in cavity endoscope detection device provided in an embodiment of the present invention
It is inside additionally provided with sorption channel, as shown in figure 4, sorption channel 125 is located between illumination channel and detection tube edges.It is i.e. of the invention
It is additionally provided in the detection pipes in cavity endoscope detection device that embodiment provides for inhaling cavity endoscope detection device
The sorption channel 125 being attached in test serum is formed negative by extracting the air in sorption channel 125 in sorption channel 125
Pressure, so that cavity endoscope detection device is adsorbed in test serum, wherein sorption channel 125 is located at illumination channel and detection
Between tube edges, that is, it is located on the outside of illumination channel, at the position of detection pipes avris.
Handle case on the basis of the various embodiments described above, in cavity endoscope detection device provided in an embodiment of the present invention
Button hole is offered on body, switching push button is provided in button hole, and switching push button is for switching different optical filters, to obtain not
The illumination optical signal of co-wavelength.The button hole of handle housing in cavity endoscope detection device i.e. provided in an embodiment of the present invention
It is inside provided with switching push button, the optical filter of optical signal can be illuminated with switch filtering different wave length by the switching push button, so that work
It can choose the illumination optical signal transmitted through the different wave length come as personnel, wherein the function of switching push button can also be by software certainly
Definition, modifies its corresponding function.
Button hole on the basis of the various embodiments described above, in cavity endoscope detection device provided in an embodiment of the present invention
It is inside additionally provided with imaged button, imaged button is for controlling the image-forming module being connected with light field fiber optic bundle to be measured before object lens
Tissue regions are imaged.In the button hole of handle housing in cavity endoscope detection device i.e. provided in an embodiment of the present invention
Be provided with imaged button, by the imaged button can control the image-forming module that is connected with light field fiber optic bundle to before object lens to
It surveys tissue regions and carries out imaging of taking pictures, wherein the function of imaged button can also be customized by software, modifies its corresponding function.
On the basis of the various embodiments described above, the absorption in cavity endoscope detection device provided in an embodiment of the present invention is logical
Road is a circular passage or several circular channels.I.e. for making cavity endoscope detection device be adsorbed on the absorption in test serum
It channel can be for one along the circular passage on the inside of detection pipes, or multiple circular channels, to form sufficiently large bear
Pressure, so that cavity endoscope detection device is adsorbed in test serum.
The embodiment of the present invention also provides a kind of three dimensional non-linear laser scanning cavity endoscope, and Fig. 5 is the embodiment of the present invention
The three dimensional non-linear laser scanning cavity endoscope structure schematic diagram one of offer, as shown in figure 5, the three dimensional non-linear laser scanning
Cavity endoscope includes:
Phosphor collection device 56, scanning collection controller 531, femtosecond pulse laser, fiber coupling module and above-mentioned
The cavity endoscope detection device 1 that each embodiment provides, phosphor collection device 56 and fiber coupling module with cavity endoscope
1 fiber optic communication of detection device connection, phosphor collection device 56 and cavity endoscope detection device 1 with scanning collection controller
531 electrical connections, in which:
Femtosecond pulse laser, for exporting pulsed laser signal to fiber coupling module；
Fiber coupling module, for coupling the pulsed laser signal of femtosecond pulse laser output, and transmission pulse laser
Signal collimation lens into cavity endoscope detection device 1；
Cavity endoscope detection device 1, after receiving pulsed laser signal, output pulsed laser signal is thin to life entity
Autofluorescence substance intracellular, and the fluorescence signal and second harmonic that generate after the excitation of autofluorescence substance are obtained by object lens
Signal, and fluorescence signal and second harmonic signal are exported to phosphor collection device 56；
Phosphor collection device 56, after receiving fluorescence signal and second harmonic signal, difference conversion fluorescence signal and two
Rd harmonic signal is corresponding electric signal；
Scanning collection controller 531 is scanned pulsed laser signal for controlling micro electromechanical scanning galvanometer, Yi Jitong
Step acquisition electric signal.
Specifically, three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention includes phosphor collection device
56, scanning collection controller 531, femtosecond pulse laser, fiber coupling module and cavity endoscope detection device 1, thus
Form the three dimensional non-linear laser scanning detected using two photon imaging technology to human body gastrointestinal tissue and oral cavity tissue
Cavity endoscope, wherein femtosecond pulse laser can be used for exciting human gastrointestinal tissue and mouth with emission pulse laser signal
Autofluorescence substance in cavity tissue cell generates multiphoton fluorescence signal and second harmonic signal, including using flying for 920nm
FAD and collagen in pulse per second (PPS) laser activated cell, excite 500-600nm fluorescence signal and 460nm it is secondary humorous
Wave signal, and pass through 780nm femtosecond pulse laser activated cell in FAD or the autofluorescences substance such as NADH, to produce
Raw corresponding fluorescence signal and second harmonic signal, wherein femtosecond pulse laser and fiber coupling module are grouped together into
Laser emitting module 540；
Wherein, phosphor collection device 56 is integrated with two paths of signals and collects optical path, respectively fluorescence signal collection optical path and two
Rd harmonic signal collects optical path, the collection respectively of Lai Shixian fluorescence signal and second harmonic signal；Scanning collection controller 531 is controlled
Micro electromechanical scanning galvanometer processed is scanned pulsed laser signal and autofluorescence substance is excited to generate fluorescence signal and secondary humorous
Wave signal, and the first electric signal and second that acquisition 56 conversion fluorescence signal of phosphor collection device and second harmonic signal obtain
Electric signal；The three dimensional non-linear laser scanning cavity endoscope can be divided into according to the difference of 1 structure of cavity endoscope detection device
Laparoscope and mouth mirror.Wherein, the resolution ratio of the three dimensional non-linear laser scanning cavity endoscope may be configured as 800nm, imaging
The visual field can be 400 microns * 400 microns, and image taking speed can be 26 frames (256*256 pixel) or 13 frames (512*512 pixel).
Three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention is adopted using phosphor collection device, scanning
Collect controller, femtosecond pulse laser, fiber coupling module and cavity endoscope detection device, utilizes two-photon to be formed
The three dimensional non-linear laser scanning cavity endoscope that imaging technique detects human body gastrointestinal tissue and oral cavity tissue, passes through
Liquid lens adjusts objective focal length, realizes the 3-D scanning of laser scanning microscope, passes through femtosecond pulse laser activated cell
Interior autofluorescence substance obtains multiphoton fluorescence signal and second harmonic signal, realizes that laser scanning microscope is non-linear, passes through
Phosphor collection device collects fluorescence signal and second harmonic signal, and is converted to corresponding electric signal, and then passes through the telecommunications
Number obtain the fluorescent image etc. of corresponding reflection cell tissue structure, wherein the use of cavity endoscope detection device is so that work
Can flexibly gastrointestinal tissue, oral cavity tissue and uterine cavity inner tissue in human abdominal cavity be detected by making personnel, to human body
, only need to be to human abdomen when gastrointestinal tissue is detected, the pain of operation cost and patient can so be reduced by opening up an osculum
Hardship can carry out Non-invasive detection when uterine cavity inner tissue is imaged by nature channel (vagina), and equipment operation is simple, user
Just.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror, further includes lighting module and image-forming module, as shown in figure 5, lighting module 534 and image-forming module 533 with peeped in cavity
Mirror detection device fiber optic communication connection, in which:
Lighting module 534 successively includes illuminating lens 5342, variable filter 5341 and lighting source 5343, illuminating lens
5342 are connected with illumination fiber optic bundle, and lighting source is for providing illumination optical signal；
Image-forming module 533 successively includes imaging len 5331 and camera 5332, imaging len 5331 and light field fiber optic bundle phase
Connection, camera 5332 is for obtaining test serum regional image information.Three dimensional non-linear laser i.e. provided in an embodiment of the present invention
Scanning cavity endoscope is additionally provided with lighting module 534 and image-forming module 533, wherein lighting module 534 successively includes illumination
Lens 5342, variable filter 5341 and lighting source 5343, wherein lighting source passes through electric variable optical filter runner, can
To switch different optical filters, to obtain the illumination optical signal of different wave length, basic principle is not interfere two-photon fluorescence imaging,
Such as obtain autofluorescence and when second harmonic, red or infrared optical filter can be switched to, with obtain 370nm,
The illumination optical signal of 635nm or infrared 850nm, 940nm, illumination optical signal enter illumination fiber optic bundle by Lens Coupling；
Image-forming module 533 includes successively imaging len and camera, and lens focus is imaged on camera, bright for directly observing
?.Wherein camera is two corresponding with binocular light field fiber optic bundle, and light field imaging and two photon imaging form multi-modal laparoscope, bright
Field binocular three-dimensional stereo laparoscope mode, carries out wide-field sample view, the basic pattern of main detection sample.It can for having
Doubtful or interested region, can switch to two-photon mode, carries out autofluorescence and Second Harmonic Imaging, observes the cell of sample
Grade form provides foundation for further judgement., wherein camera can set for the imaging based on image devices such as CCD or CMOS
It is standby.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror further includes air extractor, as shown in figure 5, air extractor 52 includes mainly aspiration pump, passes through exhaust pipe and sorption channel phase
Even, extraction valve is set in exhaust pipe, extraction valve is electrically connected with air extractor 52, and air extractor 52 is opened by adjusting extraction valve
The size closed and be opened and closed, controls the extraction flow of exhaust pipe, to realize that the pumping to sorption channel controls, and then adjusts
Negative pressure in sorption channel, so that effect of the cavity endoscope detection device by atmospheric pressure, is adsorbed on human body stomach or oral cavity
Equal tissues, reduce bio-tissue activity bring motion artifacts, so that imaging is more stable, clear.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror further includes industrial personal computer, as shown in figure 5, industrial personal computer 532 is electrically connected with scanning collection controller 531, in which:
Industrial personal computer 532 is for obtaining collected first electric signal of scanning collection controller 531 and the second electric signal, and base
The first fluorescent image is generated in the first electric signal and the second fluorescent image is generated based on the second electric signal.That is the embodiment of the present invention
The three dimensional non-linear laser scanning cavity endoscope of offer further includes the industrial personal computer 532 being electrically connected with scanning collection controller 531,
The industrial personal computer 532 is based on the first electric signal and generates the first fluorescent image and generate the second fluorescent image based on the second electric signal,
It can be respectively used to display eucaryotic cell structure and fibre structure information, control software is wherein installed on industrial personal computer, by controlling software,
Control instruction is sent to scanner, to control scanning collection controller, to obtain above-mentioned first electric signal and the second electric signal.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror further includes display, as shown in figure 5, display 55 is electrically connected with industrial personal computer 532, for showing the first fluorescent image and the
Two fluorescent images.Three dimensional non-linear laser scanning cavity endoscope i.e. provided in an embodiment of the present invention further includes for showing first
The display 55 of fluorescent image and the second fluorescent image, by display 55, staff can directly acquire the first fluorogram
The relevant information of picture and the second fluorescent image.
Wherein, Fig. 6 is three dimensional non-linear laser scanning cavity endoscope structure schematic diagram two provided in an embodiment of the present invention,
As shown in fig. 6, the three dimensional non-linear laser scanning cavity endoscope also includes:
Phosphor collection device 56, scanning collection controller 531, femtosecond pulse laser, fiber coupling module and above-mentioned
The cavity endoscope detection device 1 of each embodiment offer, air extractor 52, industrial personal computer 532, lighting module 534, image-forming module
533, phosphor collection device 56 and fiber coupling module are connect with 1 fiber optic communication of cavity endoscope detection device, phosphor collection
Device 56 and cavity endoscope detection device 1 are electrically connected with scanning collection controller 531, wherein each part mentioned above module or dress
The function of setting is identical as the apparatus function effect in the various embodiments described above, including femtosecond pulse laser and fiber coupling module group
It is combined to form laser emitting module 540, lighting module 534 successively includes illuminating lens 5342,5341 and of variable filter
Lighting source 5343, image-forming module 533 successively include imaging len and camera, the three dimensional non-linear laser scanning cavity endoscope
In cavity endoscope detection device 1 be mouth mirror detection device, include in the light channel structure of the cavity endoscope detection device
Liquid lens, acts on identical as the liquid lens for including in the various embodiments described above, and optical path is also and in the various embodiments described above
Corresponding optical path is identical.
On the basis of the various embodiments described above, Fig. 7 is phosphor collection apparatus structure schematic diagram provided in an embodiment of the present invention,
As shown in fig. 7, phosphor collection device provided in an embodiment of the present invention includes collecting fiber optic universal interface 881, the first photomultiplier transit
Pipe 882, the second photomultiplier tube 883 and positioned at collect between fiber optic universal interface 881 and the first photomultiplier tube 882 the
One collects optical path, collects optical path positioned at second collected between fiber optic universal interface 881 and the second photomultiplier tube 883, in which:
First collection optical path successively includes coupling collecting lens 81, infrared fileter 82, the filter of the first dichroscope 83, first
Mating plate 84 and the first collecting lens 85, wherein the first collection optical path is used to collect the fluorescence letter that phosphor collection device receives
Number, the first photomultiplier tube 882 is the first electric signal for conversion fluorescence signal；
Second collection optical path successively includes coupling collecting lens 81, infrared fileter 82, the first dichroscope the 83, the 2nd 2
To Look mirror 86, the second optical filter 87 and the second collecting lens 88, wherein the second collection optical path is for collecting phosphor collection device
The second harmonic signal received, the second photomultiplier tube 883 is for converting second harmonic signal as the second electric signal.That is this hair
The phosphor collection device that bright embodiment provides has simple two-way signal collecting function, is integrated with two-way optical path, wherein first collects light
The first dichroscope 83 in road is transmission fluorescence signal, the dichroscope of reflected second harmonics, the second dichroscope 86 and the
One dichroscope 83 is same dichroscope, is used for reflected second harmonics, and the first optical filter 84 is filtered for transmiting fluorescence signal
Except remaining interference signal, the second optical filter 87 filters out remaining interference signal for transmiting corresponding second harmonic signal, for example,
When using autofluorescence substance in the femto second optical fiber laser exciting human abdominal cavity 780nm or Stomatocyte, it can be obtained 390nm's
The two-photon auto flourescence signals of second harmonic signal and 450-600nm, are passed through by 420nm above wavelength, 420 or less wavelength
The dichroscope of reflection i.e. the first dichroscope 83 can separate two-way fluorescence, use the first optical filter of 390 ± 20nm respectively
The second optical filter 87 available clean second harmonic signal and fluorescence signal of 84 and 450-600nm.
Wherein, Fig. 8 is the box combination knot of three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention
The joint sealing structural schematic diagram one of structure, as shown in figure 8, it is integrated to be integrated in display 55 and the cabinet for being equipped with modules on case lid
Together, facilitate whole equipment mobile, and replacement workplace, and the display 55 is when in use, case can be placed in addition
On body, to facilitate staff to obtain the information on display, wherein in the three dimensional non-linear laser scanning cavity endoscope
Cavity endoscope detection device 1 is mouth mirror detection device.After having used the three dimensional non-linear laser scanning cavity endoscope,
Staff can portable equipment case, convenient changing workplace uses the equipment especially in hospital, laboratory or outdoor location
It can be more convenient.
Wherein, Fig. 9 is the box combination knot of three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention
The joint sealing structural schematic diagram two of structure, as shown in figure 9, it is integrated to be integrated in display 55 and the cabinet for being equipped with modules on case lid
Together, facilitate whole equipment mobile, and replacement workplace, and the display 55 is when in use, case can be placed in addition
On body, to facilitate staff to obtain the information on display, wherein in the three dimensional non-linear laser scanning cavity endoscope
Cavity endoscope detection device 1 be laparoscope detection device, and laparoscope detection device can be arranged simultaneously it is multiple.When having used
After the three dimensional non-linear laser scanning cavity endoscope, staff can portable equipment case, convenient changing workplace especially exists
Hospital, laboratory or outdoor location, can be more convenient using the equipment.
On the basis of the various embodiments described above, three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention
In cavity endoscope detection device be it is multiple.Phosphor collection device and fiber coupling module i.e. provided in an embodiment of the present invention can
It is connect simultaneously with multiple cavities endoscope detection device fiber optic communication, i.e., in a three dimensional non-linear laser scanning cavity endoscope
Multiple detection devices are integrated in system, to detect while realization to gastrointestinal tissue different parts, to compare and analyze.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror further includes adjusting optical fiber, for phosphor collection device and fiber coupling module respectively between cavity endoscope detection device
Optical fiber transmit connection, in which:
Adjust the adjustable in length of optical fiber.Three dimensional non-linear laser scanning cavity endoscope i.e. provided in an embodiment of the present invention
In phosphor collection device and fiber coupling module pass through length-adjustable adjusting optical fiber and cavity endoscope detection device respectively
Optical fiber transmission connection is carried out to carry out flexible movement detector to realize according to different experiments scene needs, avoid limited optical fiber
The limitation of length, wherein the adjustable in length of optical fiber is adjusted, to realize various occasions by the optical fiber for replacing different length
Using the optical fiber that can carry out different length at any time as needed is replaced.
For the three dimensional non-linear laser scanning cavity endoscope that the various embodiments described above provide, the embodiment of the present invention is also provided
Another specific embodiment, Figure 10 provide the platform of three dimensional non-linear laser scanning cavity endoscope for the embodiment of the present invention
Formula structural schematic diagram one, as shown in Figure 10, the three dimensional non-linear laser scanning cavity endoscope include that air extractor 52, first fills
Set 53, second device 54, display 55 and cavity endoscope detection device 1, wherein be integrated with scanning in first device 53 and adopt
Collection controller and industrial personal computer, industrial personal computer are electrically connected with display 55, and second device 54 is integrated with femtosecond pulse laser, optical fiber coupling
Mold block and phosphor collection device, lighting module and image-forming module, fiber coupling module and phosphor collection device with absorption
51 optical fiber of formula microscope detection device transmission connection, wherein cavity endoscope detection device 1 is mouth mirror detection device, is used for
Human oral cavity tissue is detected, whether there is or not Cancer residuals etc. to believe with the good pernicious, invasive depth, transfer case and the incisxal edge that understand tumour
Breath, the absorption type three dimensional non-linear laser scanning microscope working principle is identical as the various embodiments described above, and details are not described herein again.
Wherein, Figure 11 embodiment of the present invention provides the mesa structure schematic diagram of three dimensional non-linear laser scanning cavity endoscope
Two, as shown in figure 11, which also includes air extractor 52, first device 53, second
Device 54, display 55 and cavity endoscope detection device 1, wherein scanning collection controller is integrated in first device 53
And industrial personal computer, industrial personal computer are electrically connected with display 55, second device 54 be integrated with femtosecond pulse laser, fiber coupling module and
Phosphor collection device, lighting module and image-forming module, fiber coupling module and phosphor collection device with absorption type microscope
51 optical fiber of detection device transmission connection, wherein cavity endoscope detection device 1 is laparoscope detection device, laparoscope detection
Device is embedded in human abdomen, detects to gastrointestinal tissue, with understand the good pernicious, invasive depth of tumour, transfer case and
Whether there is or not the information such as Cancer residual for incisxal edge.
Above-mentioned, although the foregoing specific embodiments of the present invention is described with reference to the accompanying drawings, not to invention protection scope
Limitation, those skilled in the art should understand that, based on the technical solutions of the present invention, those skilled in the art are not required to
The above is only preferred embodiment of the present application, are not intended to restrict the invention, for those skilled in the art, this
Application can have various modifications and variations.Within the spirit and principles of this application, made any modification, equivalent replacement,
Improve etc., it should be included within the scope of protection of this application.
The apparatus embodiments described above are merely exemplary, wherein unit can be as illustrated by the separation member
Or may not be and be physically separated, component shown as a unit may or may not be physical unit, i.e.,
It can be located in one place, or may be distributed over multiple network units.It can select according to the actual needs therein
Some or all of the modules achieves the purpose of the solution of this embodiment.Those of ordinary skill in the art are not paying creative labor
In the case where dynamic, it can understand and implement.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: it still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features；
And these are modified or replaceed, technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution spirit and
Range.
Claims (10)
1. a kind of cavity endoscope detection device characterized by comprising
Handle housing and detection pipes, the handle housing are fixedly connected with the detection pipes, and spy is provided in the detection pipes
Channel to be surveyed, is provided with relay lens and object lens in the detection channels, the object lens are located at the passway of the detection channels,
The light channel structure being arranged in the object lens, the relay lens and the handle housing forms the first optical path and the second optical path, described
The axle center of detection channels is overlapped with the detection pipes axle center, in which:
First optical path successively includes the collimation between the handle housing inner fiber general-purpose interface and the passway
Lens, micro electromechanical scanning galvanometer, lens, dichroscope, the relay lens and the object lens, wherein first optical path is used for
It conducts the received laser signal of the collimation lens and is interfaced to the passway from the fiber optic universal；
During second optical path successively includes object lens between the passway and the fiber optic universal interface, is described
After mirror and the dichroscope, wherein second optical path is for conducting the collected optical signal of the object lens from the channel
Mouthful to the fiber optic universal interface.
2. cavity endoscope detection device according to claim 1, which is characterized in that the light channel structure further includes that liquid is saturating
Mirror, the liquid lens is between the collimation lens and the micro electromechanical scanning galvanometer, to form the first new optical path, institute
State the first new optical path successively and include collimation lens, the liquid between the fiber optic universal interface and the passway
Lens, the micro electromechanical scanning galvanometer, the lens, the dichroscope and the object lens.
3. cavity endoscope detection device according to claim 1 or claim 2, which is characterized in that be additionally provided in the detection pipes
Several illumination channels, it is described to illuminate the illumination fiber optic bundle for being provided in channel and being used for transmission illumination optical signal, wherein the illumination
Channel is uniformly distributed centered on the axle center of the detection channels.
4. cavity endoscope detection device according to claim 3, which is characterized in that be additionally provided with sight in the detection pipes
Channel is surveyed, the observation channel is located between the detection channels and the illumination channel, in which:
Observation camera lens, the bright field light in the observation camera lens and the observation channel are provided at the passway in the observation channel
Fine beam is connected, to obtain the test serum regional image information before the object lens.
5. cavity endoscope detection device according to claim 3, which is characterized in that be additionally provided with suction in the detection pipes
Attached channel, the sorption channel is between the illumination channel and the detection tube edges.
6. cavity endoscope detection device according to claim 4, which is characterized in that offered on the handle housing by
Buttonhole is provided with switching push button in the button hole, and the switching push button is for switching different optical filters, to obtain different waves
The long illumination optical signal.
7. cavity endoscope detection device according to claim 6, which is characterized in that be additionally provided with into the button hole
As button, the imaged button is for controlling the image-forming module being connected with the light field fiber optic bundle to be measured before the object lens
Tissue regions are imaged.
8. a kind of three dimensional non-linear laser scanning cavity endoscope characterized by comprising
Phosphor collection device, scanning collection controller, femtosecond pulse laser, fiber coupling module and claim 1-7 appoint
Cavity endoscope detection device described in one, the phosphor collection device and the fiber coupling module are and in the cavity
Sight glass detection device fiber optic communication connection, the phosphor collection device and the cavity endoscope detection device with the scanning
Acquisition controller electrical connection, in which:
The femtosecond pulse laser, for exporting pulsed laser signal to the fiber coupling module；
The fiber coupling module for coupling the pulsed laser signal of the femtosecond pulse laser output, and transmits
Collimation lens described in the pulsed laser signal to the cavity endoscope detection device；
The cavity endoscope detection device exports the pulsed laser signal extremely after receiving the pulsed laser signal
The intracellular autofluorescence substance of life entity, and obtained by the object lens generate after autofluorescence substance excitation it is glimmering
Optical signal and second harmonic signal, and the fluorescence signal and the second harmonic signal are exported to the phosphor collection device；
The phosphor collection device is converted described glimmering respectively after receiving the fluorescence signal and the second harmonic signal
Optical signal and the second harmonic signal are corresponding electric signal；
The scanning collection controller is scanned the pulsed laser signal for controlling the micro electromechanical scanning galvanometer,
And electric signal described in synchronous acquisition.
9. three dimensional non-linear laser scanning cavity endoscope according to claim 8, which is characterized in that further include illumination mould
Block and image-forming module, the lighting module and the image-forming module connect with the cavity endoscope detection device fiber optic communication
It connects, in which:
The lighting module successively includes illuminating lens, variable filter and lighting source, the illuminating lens and lighting fiber
Beam is connected, and the lighting source is for providing illumination optical signal；
The image-forming module successively includes imaging len and camera, and the imaging len is connected with light field fiber optic bundle, the phase
Machine is for obtaining test serum regional image information.
10. three dimensional non-linear laser scanning cavity endoscope according to claim 8, which is characterized in that the fluorescence is received
Acquisition means include collecting fiber optic universal interface, the first photomultiplier tube, the second photomultiplier tube and being located at the collection optical fiber
First between general-purpose interface and first photomultiplier tube collects optical path, is located at the collection fiber optic universal interface and described
Second between second photomultiplier tube collects optical path, in which:
It is described first collection optical path successively include coupling collecting lens, infrared fileter, the first dichroscope, the first optical filter with
And first collecting lens, wherein the first collection optical path for collecting the fluorescence signal that phosphor collection device receives,
First photomultiplier tube is for converting the fluorescence signal as the first electric signal；
It is described second collection optical path successively include the coupling collecting lens, the infrared fileter, first dichroscope,
Second dichroscope, the second optical filter and the second collecting lens, wherein the second collection optical path is for collecting phosphor collection
The second harmonic signal that device receives, second photomultiplier tube is for converting the second harmonic signal as second
Electric signal.
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| CN107456202A (en) * | 2017-10-01 | 2017-12-12 | 凝辉(天津)科技有限责任公司 | A kind of nonlinear optics flexible endoscope imaging device |
| CN108937824A (en) * | 2018-08-02 | 2018-12-07 | 深圳大学 | A kind of acquisition methods of based endoscopic imaging device and Fluorescence lifetime distribution image |
| CN210055956U (en) * | 2019-01-31 | 2020-02-14 | 北京超维景生物科技有限公司 | Cavity endoscope detection device and three-dimensional nonlinear laser scanning cavity endoscope |
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