CN210055956U

CN210055956U - Cavity endoscope detection device and three-dimensional nonlinear laser scanning cavity endoscope

Info

Publication number: CN210055956U
Application number: CN201920180629.7U
Authority: CN
Inventors: 吴润龙; 王爱民; 江文茂; 吴丹磊; 胡炎辉; 徐阳阳; 程和平
Original assignee: Beijing Chaowei Landscape Biology Technology Co Ltd
Current assignee: Beijing Chaowei Landscape Biology Technology Co Ltd
Priority date: 2019-01-31
Filing date: 2019-01-31
Publication date: 2020-02-14
Anticipated expiration: 2029-01-31

Abstract

The embodiment of the utility model provides a cavity endoscope detection device and three-dimensional nonlinear laser scanning cavity endoscope. The cavity endoscope detection device comprises a handle shell and a detection tube, wherein a relay lens and an objective lens are arranged in a detection channel of the detection tube, a first light path and a second light path are formed, and the first light path comprises a collimating lens, a micro-electro-mechanical scanning galvanometer, a lens, a dichroic mirror, a relay lens and an objective lens; the second optical path includes an objective lens, a relay lens, and a dichroic mirror. The embodiment of the utility model provides a cavity endoscope detection device and three-dimensional nonlinear laser scanning cavity endoscope adopt and set up first light path and second light path at the inner space of handle casing and detecting tube, realize the excitation and the collection of two-photon and second harmonic signal to autofluorescence material, wherein, the adoption of relay mirror makes the detecting tube can prolong as required to satisfy the detection needs to gastrointestinal tract tissue and oral cavity tissue etc., easy operation, convenient to use.

Description

Cavity endoscope detection device and three-dimensional nonlinear laser scanning cavity endoscope

Technical Field

The embodiment of the invention relates to the technical field of laser scanning endoscopes, in particular to a cavity endoscope detection device and a three-dimensional nonlinear laser scanning cavity endoscope.

Background

Gastrointestinal malignancies are the second leading cause of cancer death in the population of developed countries and this trend has become more evident in recent years. The treatment of gastrointestinal malignant tumor mainly adopts surgical radical resection, but the specific range of the surgical resection needs to be determined when the surgical radical resection is specifically carried out, so before the operation, the benign and malignant tumor, the infiltration depth, the metastasis condition, the existence of cancer residue at the cut edge and the like need to be known. Preoperative gastrointestinal endoscopic biopsy is therefore a very important diagnostic evidence for histological diagnosis of gastrointestinal tumors. The operative form of stomach cancer is divided into full stomach resection, secondary stomach resection, partial gastrectomy, endoscopic mucosal or submucosal resection and the like according to the size, the growth position, the infiltration depth and the like of a tumor body.

Currently, gastrointestinal endoscopic biopsy is usually based on gastrointestinal endoscopy, imaging is performed with the aid of CT, MRI, etc., or a number of gastrointestinal diseases are evaluated with a conventional white light laparoscope or endoscope.

However, there are some inevitable disadvantages in imaging by imaging using CT, MRI, etc. based on enteroscopy, such as easy bleeding of intestinal tube or tumor body in operation, manual pulling or squeezing, delay in time due to repeated endoscopic biopsy when enteroscopy cannot pass through the intestinal tube, and additional emergency hemostasis if severe bleeding is caused. In clinical practice, auxiliary examination means such as CT and MRI cannot accurately judge the infiltration depth of early gastrointestinal tumors and lymph node metastasis. The gastrointestinal tumor T stage is judged by ultrasonic endoscopy, and the accuracy reported in the literature is only 44.7% -78%, which is not enough to become a reliable diagnostic standard. The ultrasonic endoscope also has poor preoperative evaluation effect on the local resection, cannot accurately subdivide gastrointestinal mucosa levels, and has poor N staging effect. Therefore, in view of the current gastrointestinal auxiliary diagnostic technology, a new gastrointestinal tumor diagnostic device is urgently needed to detect the gastrointestinal tissue information in situ and in real time.

Disclosure of Invention

Aiming at the technical problems in the prior art, the embodiment of the invention provides a cavity endoscope detection device and a three-dimensional nonlinear laser scanning cavity endoscope.

In a first aspect, an embodiment of the present invention provides a cavity endoscope detection device, including:

handle casing and detecting tube, the handle casing with detecting tube fixed connection, be provided with the detection passageway in the detecting tube, be provided with relay lens and objective in the detection passageway, objective is located the passageway department of detection passageway, objective the relay lens with the light path structure that sets up in the handle casing forms first light path and second light path, the axle center of detecting the passageway with detecting tube axle center coincidence, wherein:

the first optical path sequentially comprises a collimating lens, a micro-electromechanical scanning galvanometer, a lens, a dichroic mirror, a relay lens and the objective lens, wherein the collimating lens, the micro-electromechanical scanning galvanometer, the lens, the dichroic mirror, the relay lens and the objective lens are positioned between the optical fiber general interface and the channel port in the handle shell, and the first optical path is used for conducting a laser signal received by the collimating lens from the optical fiber general interface to the channel port;

the second optical path sequentially includes the objective lens, the relay lens, and the dichroic mirror, which are located between the channel port and the optical fiber universal interface, where the second optical path is used to conduct the optical signal collected by the objective lens from the channel port to the optical fiber universal interface.

In a second aspect, an embodiment of the present invention provides a three-dimensional nonlinear laser scanning cavity endoscope, including:

the cavity endoscope detection device comprises a fluorescence collection device, a scanning acquisition controller, a femtosecond pulse laser, an optical fiber coupling module and the cavity endoscope detection device provided by the first aspect of the embodiment of the invention, wherein the fluorescence collection device and the optical fiber coupling module are in optical fiber communication connection with the cavity endoscope detection device, and the fluorescence collection device and the cavity endoscope detection device are electrically connected with the scanning acquisition controller, wherein:

the femtosecond pulse laser is used for outputting pulse laser signals to the optical fiber coupling module;

the fiber coupling module is used for coupling the pulse laser signal output by the femtosecond pulse laser and transmitting the pulse laser signal to the collimating lens in the cavity endoscope detection device;

the cavity endoscope detection device is used for receiving the pulse laser signal, outputting the pulse laser signal to an autofluorescent substance in a cell of a living body, acquiring a fluorescence signal and a second harmonic signal generated after the autofluorescent substance is excited through the objective lens, and outputting the fluorescence signal and the second harmonic signal to the fluorescence collection device;

the fluorescence collecting device is used for receiving the fluorescence signal and the second harmonic signal and then respectively converting the fluorescence signal and the second harmonic signal into corresponding electric signals;

and the scanning acquisition controller is used for controlling the micro-electromechanical scanning galvanometer to scan the pulse laser signals and synchronously acquire the electric signals.

The cavity endoscope detection device provided by the embodiment of the invention adopts a construction mode that a handle shell and a detection tube are fixedly connected, a first light path and a second light path are arranged in the inner space of the two components to form the handheld cavity endoscope detection device, wherein the first light path comprises a collimating lens, a micro-electro-mechanical scanning galvanometer, a lens, a dichroic mirror, a relay mirror and an objective lens and is used for conducting a laser signal for exciting a spontaneous fluorescent substance; the second light path includes objective and the dichroic mirror for collect two-photon signal and second harmonic signal, wherein, the adoption of relay makes the detecting tube prolong as required, in order to satisfy the detection needs to stomach intestine tissue in the abdominal cavity, oral cavity tissue and tissue in the uterine cavity, easy operation, convenient to use.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and those skilled in the art can also obtain other drawings according to the drawings without creative efforts.

FIG. 1 is a first schematic structural diagram of a cavity endoscope detection device according to an embodiment of the present invention;

FIG. 2 is a schematic structural diagram of a cavity endoscope detection device according to an embodiment of the present invention;

FIG. 3 is a third schematic structural view of a cavity endoscope detection device provided by the embodiment of the present invention;

FIG. 4 is a fourth structural schematic view of a cavity endoscope detecting device provided by the embodiment of the present invention;

FIG. 5 is a first schematic view of a three-dimensional nonlinear laser scanning cavity endoscope according to an embodiment of the present invention;

FIG. 6 is a schematic view of a second endoscope structure in a three-dimensional nonlinear laser scanning cavity according to an embodiment of the present invention;

FIG. 7 is a schematic structural diagram of a fluorescence collection device according to an embodiment of the present invention;

FIG. 8 is a first box sealing structure diagram of a box combination structure of a three-dimensional nonlinear laser scanning cavity endoscope according to an embodiment of the present invention;

fig. 9 is a second box sealing structure diagram of the box combination structure of the three-dimensional nonlinear laser scanning cavity endoscope according to the embodiment of the present invention;

FIG. 10 is a first schematic diagram of a table-top configuration of a three-dimensional nonlinear laser scanning cavity endoscope according to an embodiment of the present invention;

FIG. 11 is a second schematic view of a desktop configuration of a three-dimensional nonlinear laser scanning endoscope.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

At present, the gastrointestinal endoscope is taken as a basis, imaging is carried out by taking CT, MRI and the like as assistance to obtain relevant information of malignancy, infiltration depth, metastasis condition, whether cancer residue exists at incisal margin and the like of tumors, and the method has some defects in specific operation, such as easy bleeding of intestinal tracts or tumor bodies, need of manual drawing or extrusion, delay of time due to repeated endoscopic biopsy when the gastrointestinal endoscope cannot pass through the intestinal tracts, and need of additional emergency hemostasis if severe bleeding is caused. In clinical practice, auxiliary examination means such as CT and MRI cannot accurately judge the infiltration depth of early gastrointestinal tumors and lymph node metastasis. The gastrointestinal tumor T stage is judged by the ultrasonic endoscope, the accuracy reported in the literature is only 44.7% -78%, the judgment is not enough to become a reliable diagnosis standard, the preoperative judgment effect of the ultrasonic endoscope on the local resection operation is poor, the gastrointestinal mucosa levels cannot be accurately subdivided, and the N stage effect is also poor.

While conventional white light laparoscopes and endoscopes can assess many gastrointestinal diseases, the technique is limited to detecting gross morphological changes. Although suspicious regions are easily found, these techniques are associated with false positive rates, specificity, and the like, as compared to in vivo detection techniques. White light endoscopy is associated with wide error in diagnosis of microscopic changes, including examination diagnosis of ulcerative colitis or Barrett's esophagus and flat adenomatous dysplasia. Confocal endoscopy combines laser technology, fluorescence detection technology, rapid scanning technology and the like, and is concerned widely because the confocal endoscope can detect mucosal changes at a microscopic level and can possibly be used for replacing tissue biopsy, and the imaging technology has high sensitivity and specificity. However, the confocal endoscopic imaging technology is still limited by the imaging depth and the fluorescent staining agent, because the gastrointestinal sample has strong absorption and scattering to visible light, the imaging depth is only on the superficial layer, and a specific fluorescent staining developer is required to be injected, the operation is too complicated, and the relevant information such as the infiltration depth of the tumor, the metastasis condition, the existence of cancer residue at the surgical incisal margin and the like cannot be accurately obtained.

The two-photon microscopic imaging technology adopts a femtosecond pulse laser with longer wavelength as an excitation light source, has the characteristics of deep imaging depth, small light damage, small photobleaching area, high fluorescence collection efficiency and the like, and has epoch-making significance in deep imaging of biological tissues. The first two-photon fluorescence microscope in the world was developed by w.denk et al at cornell university in 1990, using a multi-photon microscopic imaging technique based on nonlinear optics and femtosecond pulsed laser. The technology can rapidly obtain the tissue structure and the cell morphology of the specimen in real time by utilizing autofluorescence generated by cells in living tissues and second harmonic generated by collagen tissues. As early as 1986, the second harmonic was used for skin studies and coronary microscopic imaging studies, confirming its feasibility for being used to observe biological tissues. MPM is also an important tool for cancer research. The autofluorescence generated by the cell is derived from Nicotinamide Adenine Dinucleotide (NADH) and Flavin Adenine Dinucleotide (FAD) in the cell, the wavelength emitted by NADH is 460nm, and the second oscillation harmonic of collagen is 370-390 nm, so that a multiphoton microscope with a range of 780-940 nm is usually selected when observing tumor sample tissues. Not only does MPM imaging match standard tumor histopathology, it also provides additional information about the tumor neogenesis process, as can the metabolic levels of tumor tissue cells by measuring the ratio NADH/FAD.

With multiphoton imaging techniques, multiphoton microscopy is capable of providing real-time gastrointestinal tract tissue structure and cell morphology information. The multi-photon imaging technology has the characteristics of no need of exogenous labeled tissues, extremely sensitivity to collagen, small photodamage to tissues, deep penetration depth and the like, and can be possibly applied to optical biopsy of gastrointestinal tumors. There are no clinically available two-photon laparoscopes and endoscopes, and no two-photon imaging-based intracavity scope detection device to detect gastrointestinal tract tissue information in situ and in real time.

In order to detect gastrointestinal tract tissue information in situ in real time, an embodiment of the present invention provides a cavity endoscope detection device, fig. 1 is a schematic structural diagram of the cavity endoscope detection device provided in the embodiment of the present invention, as shown in fig. 1, the cavity endoscope detection device includes:

handle casing 11 and detecting tube 12, handle casing 11 and detecting tube 12 fixed connection are provided with the detection passageway in the detecting tube 12, are provided with relay lens 122 and objective 121 in the detection passageway, and objective 121 is located the passageway department of detecting the passageway, and the light path structure that sets up in objective 121, relay lens 122 and the handle casing 11 forms first light path and second light path, detects the axle center and the coincidence of detecting tube 12 axle center of passageway, wherein:

the first optical path sequentially comprises a collimating lens 112, a micro-electromechanical scanning galvanometer 114, a lens 115, a dichroic mirror 116, a relay mirror 122 and an objective lens 121, which are positioned between the optical fiber universal interface 111 and the passage port in the handle shell 11, wherein the first optical path is used for conducting the laser signal received by the collimating lens 112 from the optical fiber universal interface 111 to the passage port;

the second optical path sequentially includes an objective lens 121, a relay lens 122 and a dichroic mirror 116, which are located between the passage port and the universal fiber interface 111, where the second optical path is used to conduct the optical signal collected by the objective lens 121 from the passage port to the universal fiber interface 111.

Specifically, the cavity endoscope detection device provided by the embodiment of the invention integrates two optical paths, one is a first optical path for conducting a laser signal, and the laser signal is mainly used for exciting a spontaneous fluorescent substance; the other is to collect and transmit two-photon signals and second harmonic signals generated after the spontaneous fluorescent substance is excited; the two optical paths include a collimating lens 112, a micro-electromechanical scanning galvanometer 114, a lens 115 and a dichroic mirror 116, which are integrated in the handle housing 11, a relay lens 122 and an objective lens 121 are integrated in the detection tube 12, the objective lens 121 is disposed at a channel port of a detection channel, the relay lens 122 is disposed inside the objective lens 121 and disposed in the detection channel, the relay lens 122 is used for conducting a two-photon signal and a second harmonic signal collected by the objective lens 121 to the dichroic mirror 116 in a long distance, an image plane of the objective lens 121 coincides with a focal plane of the relay lens 122, the relay lens is used for image transmission firstly, and a laser signal scanning area passing through the micro-electromechanical scanning mirror is conducted to an image plane of the objective lens 121 in an equal ratio of 1: 1. Secondly, the relay lens also conducts the optical signal collected by the objective lens 121 to the optical path structure part in the handle shell and collects the optical signal through the optical fiber bundle of the optical path structure, wherein the dichroic mirror 116 can be a long-pass short-dichroic mirror 116 or a short-pass long-dichroic mirror 116, that is, when the long-pass short-dichroic mirror 116 is arranged, a pulse laser signal for exciting the autofluorescence is transmitted, and the collected two-photon signal and the second harmonic signal are reflected, as shown in fig. 1, at this time, the endoscopic detection device in the cavity can be a detection device of a laparoscope; fig. 2 is a schematic structural diagram of a second cavity endoscope detection device according to an embodiment of the present invention, as shown in fig. 2, when the dichroic mirror is a short-pass long-pass dichroic mirror, the dichroic mirror reflects a pulse laser signal for exciting a spontaneous fluorescent substance, transmits the collected two-photon signal and a second harmonic signal, reflects a laser signal emitted from the optical fiber universal interface 111 and incident on the dichroic mirror 116 after passing through the collimating lens 112, the micro-electromechanical scanning mirror 114, and the lens 115 to the objective lens 121, and transmits the two-photon signal and the second harmonic signal collected by the objective lens 121, at this time, the cavity endoscope detection device may be a detection device of a mouth mirror, where the detection device of the mouth mirror also includes two parts, namely, a handle housing 11 and a detection tube 12.

The cavity endoscope detection device provided by the embodiment of the invention adopts a construction mode that a handle shell and a detection tube are fixedly connected, a first light path and a second light path are arranged in the inner space of the two components to form the handheld cavity endoscope detection device, and the first light path comprises a collimating lens, a liquid lens, a micro-electro-mechanical scanning galvanometer, a lens, a dichroic mirror, a relay mirror and an objective lens and is used for conducting and exciting a laser signal of an autofluorescence substance; the second light path comprises an objective lens and a dichroic mirror and is used for collecting two-photon signals and second harmonic signals, wherein the detection tube can be prolonged as required by the adoption of the relay lens, so that the detection requirements of gastrointestinal tract tissues and oral tissues are met, and the device is simple in operation and convenient to use.

On the basis of the foregoing embodiments, the optical path structure in the cavity endoscope detection device provided in the embodiment of the present invention further includes a liquid lens, fig. 3 is a schematic structural diagram three of the cavity endoscope detection device provided in the embodiment of the present invention, as shown in fig. 3, the liquid lens 113 is located between the collimating lens 112 and the micro-electromechanical scanning galvanometer 114 to form a new first optical path, and the new first optical path sequentially includes the collimating lens 112, the liquid lens 113, the micro-electromechanical scanning galvanometer 114, the lens 115, the dichroic mirror 116, and the objective lens 121, which are located between the optical fiber universal interface 111 and the passage port. That is, the liquid lens 113 is disposed such that the surface of the liquid lens 113 can be correspondingly curved by applying a voltage or a current to the liquid lens 113, and thus parallel light emitted from the collimating lens 112 can be aligned to generate different powers. The specific light path is as follows: laser signals are emitted from the optical fibers, parallelly enter the liquid lens 113 after passing through the collimating lens 112, corresponding focal power is generated from the liquid lens 113 according to loaded voltage or current signals, and emitted convergent or divergent light passes through the micro-electro-mechanical scanning galvanometer 114, the lens 115 and the dichroic mirror 116, is transmitted to the objective lens 121 through the relay lens 122 and is converged on a sample. The focal power change introduced by the liquid lens 113 can make the focal point of the laser signal emitted from the opening of the objective lens 121 move back and forth in the depth direction, and the liquid lens 113 has a very fast response speed, and the scanning frequency is in the order of KHz, so that fast depth direction scanning imaging can be realized. The liquid lens 113 is equivalent to parallel plate glass when no voltage or current signal is applied, and has no focal power to the laser signal and does not cause any shift of the focus behind the objective lens 121, thereby realizing three-dimensional stereo imaging.

On the basis of the above embodiments, a plurality of illumination channels are further disposed in the probe tube of the endoscopic cavity detection device provided in the embodiment of the present invention, fig. 4 is a schematic structural diagram of the endoscopic cavity detection device provided in the embodiment of the present invention, and as shown in fig. 4, an illumination fiber bundle for transmitting an illumination light signal is disposed in the illumination channel 123, wherein the illumination channel 123 is uniformly distributed with the axis of the probe channel as the center. That is, the detection tube in the cavity endoscope detection device provided by the embodiment of the present invention is further provided with a plurality of illumination channels 123, more than one illumination channel 123 is provided, each illumination channel is provided with an illumination fiber bundle, the illumination fibers have a certain aperture angle, and can be directly used for divergent illumination without a lens, and the illumination channels 123 are uniformly distributed with the axis of the detection channel as the center, so as to provide uniform illumination for the cavity endoscope detection device, thereby facilitating the work and observation of the state of the tissue region to be detected in front of the objective lens.

On the basis of the above embodiments, the detection tube in the cavity endoscope detection device provided by the embodiment of the present invention is further provided with an observation channel, as shown in fig. 4, the observation channel is located between the detection channel and the illumination channel, wherein:

an observation lens 124 is arranged at the channel opening of the observation channel, and the observation lens 124 is connected with the bright field optical fiber bundle in the observation channel to acquire image information of the tissue area to be measured in front of the objective lens. That is, the detection tube in the cavity endoscope detection device provided by the embodiment of the present invention is further provided with an observation channel, the observation channel is located between the detection channel and the illumination channel, and is provided with an observation lens 124 and a bright field optical fiber bundle, the bright field optical fiber bundle is an imaging optical fiber bundle and is used for transmitting image information of a tissue area to be detected in front of the objective lens captured by the observation lens 124, wherein one or two observation channels may form binocular observation, so as to realize a function of the stereo bright field cavity endoscope.

On the basis of the above embodiments, the probe tube in the endoscopic cavity detection apparatus provided by the embodiment of the present invention further includes an absorption channel, as shown in fig. 4, the absorption channel 125 is located between the illumination channel and the edge of the probe tube. That is, the probe tube in the endoscopic cavity probe device provided in the embodiment of the present invention further has an adsorption channel 125 for adsorbing the endoscopic cavity probe device to the tissue to be detected, and negative pressure is formed in the adsorption channel 125 by extracting air in the adsorption channel 125, so that the endoscopic cavity probe device is adsorbed to the tissue to be detected, wherein the adsorption channel 125 is located between the illumination channel and the edge of the probe tube, that is, located outside the illumination channel and near the side of the probe tube.

On the basis of the above embodiments, the handle casing of the cavity endoscope detection device provided by the embodiments of the present invention is provided with a button hole, and a switch button is disposed in the button hole and used for switching different optical filters to obtain illumination light signals with different wavelengths. Namely, the switch button is arranged in the button hole of the handle shell in the cavity endoscope detection device provided by the embodiment of the invention, and the optical filters for filtering the illumination light signals with different wavelengths can be switched by the switch button, so that the worker can select the transmitted illumination light signals with different wavelengths, and the function of the switch button can be customized by software to modify the corresponding function.

On the basis of the above embodiments, the button hole in the cavity endoscope detection device provided in the embodiments of the present invention is further provided with an imaging button, and the imaging button is used to control an imaging module connected to the brightfield optical fiber bundle to image a tissue region to be detected in front of the objective lens. Namely, the imaging button is arranged in the button hole of the handle shell in the cavity endoscope detection device provided by the embodiment of the invention, the imaging module connected with the bright field optical fiber bundle can be controlled by the imaging button to photograph and image the tissue area to be detected in front of the objective lens, and the function of the imaging button can be customized by software to modify the corresponding function.

On the basis of the above embodiments, the suction channel in the endoscopic cavity detection device provided by the embodiments of the present invention is an annular channel or several circular channels. The adsorption channel used for adsorbing the cavity endoscope detection device on the tissue to be detected can be an annular channel along the inner side of the detection tube or a plurality of circular channels to form enough negative pressure, so that the cavity endoscope detection device is adsorbed on the tissue to be detected.

The embodiment of the present invention further provides a three-dimensional nonlinear laser scanning cavity endoscope, fig. 5 is a schematic structural diagram of the three-dimensional nonlinear laser scanning cavity endoscope provided in the embodiment of the present invention, as shown in fig. 5, the three-dimensional nonlinear laser scanning cavity endoscope includes:

fluorescence collection device 56, scanning acquisition controller 531, femto second pulse laser, fiber coupling module and the cavity endoscope detection device 1 that each above-mentioned embodiment provided, fluorescence collection device 56 and fiber coupling module all with cavity endoscope detection device 1 optical fiber communication connection, fluorescence collection device 56 and cavity endoscope detection device 1 all with scanning acquisition controller 531 electricity be connected, wherein:

the optical fiber coupling module is used for coupling a pulse laser signal output by the femtosecond pulse laser and transmitting the pulse laser signal to a collimating lens in the cavity endoscope detection device 1;

the cavity endoscope detection device 1 is used for receiving the pulse laser signal, outputting the pulse laser signal to the autofluorescence substance in the living body cell, acquiring a fluorescence signal and a second harmonic signal generated after the autofluorescence substance is excited through an objective lens, and outputting the fluorescence signal and the second harmonic signal to the fluorescence collection device 56;

a fluorescence collecting device 56 for receiving the fluorescence signal and the second harmonic signal and converting the fluorescence signal and the second harmonic signal into corresponding electrical signals;

and the scanning acquisition controller 531 is used for controlling the micro-electromechanical scanning galvanometer to scan the pulse laser signals and synchronously acquire electric signals.

Specifically, the three-dimensional nonlinear laser scanning cavity endoscope provided by the embodiment of the present invention comprises a fluorescence collecting device 56, a scanning collecting controller 531, a femtosecond pulse laser, a fiber coupling module and a cavity endoscope detecting device 1, thereby forming a three-dimensional nonlinear laser scanning cavity endoscope for detecting gastrointestinal tissues and oral tissues of a human body by using a two-photon imaging technology, wherein the femtosecond pulse laser can emit pulse laser signals for exciting autofluorescence substances in gastrointestinal tissues and oral tissue cells of the human body, generating multiphoton fluorescence signals and second harmonic signals, including using a 920nm femtosecond pulse laser to excite FAD and collagen in cells, exciting a 500-600nm fluorescence signal and a 460nm second harmonic signal, and exciting autofluorescence substances such as FAD and NADH in cells by a 780nm femtosecond pulse laser, to generate corresponding fluorescence signal and second harmonic signal, wherein the femtosecond pulse laser and the fiber coupling module are combined together to form a laser emission module 540;

the fluorescence collecting device 56 integrates two signal collecting optical paths, namely a fluorescence signal collecting optical path and a second harmonic signal collecting optical path, to respectively collect the fluorescence signal and the second harmonic signal; the scanning acquisition controller 531 controls the micro-electromechanical scanning galvanometer to scan the pulse laser signals and excite the autofluorescence substance to generate fluorescence signals and second harmonic signals, and acquires first electric signals and second electric signals obtained by converting the fluorescence signals and the second harmonic signals by the fluorescence collecting device 56; the three-dimensional nonlinear laser scanning cavity endoscope can be divided into a laparoscope and a stomatoscope according to the difference of the structure of the cavity endoscope detection device 1. The resolution of the three-dimensional nonlinear laser scanning cavity endoscope can be set to 800nm, the imaging field of view can be 400 micrometers by 400 micrometers, and the imaging speed can be 26 frames (256 pixels by 256) or 13 frames (512 pixels by 512 pixels).

The invention provides a three-dimensional nonlinear laser scanning cavity endoscope, which adopts a fluorescence collecting device, a scanning collecting controller, a femtosecond pulse laser, an optical fiber coupling module and a cavity endoscope detecting device, thereby forming the three-dimensional nonlinear laser scanning cavity endoscope for detecting human gastrointestinal tissues and oral tissues by utilizing a two-photon imaging technology, adjusting the focal length of an objective lens through a liquid lens to realize the three-dimensional scanning of a laser scanning microscope, exciting intracellular autofluorescence substances through the femtosecond pulse laser to obtain multiphoton fluorescence signals and second harmonic signals to realize the nonlinearity of the laser scanning microscope, collecting the fluorescence signals and the second harmonic signals through the fluorescence collecting device, converting the fluorescence signals into corresponding electric signals, and further obtaining corresponding fluorescence images reflecting the cell tissue structure and the like through the electric signals, wherein the adoption of the cavity endoscope detecting device enables a worker to flexibly perform the detection on the gastrointestinal tissues in the abdominal cavity of the human body, Oral cavity tissue and palace intracavity tissue are surveyed, when surveying human intestines and stomach tissue, only need to set up a osculum and can so reduce operation cost and patient's misery, when organizing the formation of image in the palace intracavity, accessible nature passageway (vagina) carries out non-invasive detection, equipment easy operation, convenient to use.

On the basis of the foregoing embodiments, the three-dimensional nonlinear laser scanning cavity endoscope provided in the embodiments of the present invention further includes an illumination module and an imaging module, as shown in fig. 5, the illumination module 534 and the imaging module 533 are both connected to the cavity endoscope detection device through optical fiber communication, where:

the illumination module 534 sequentially comprises an illumination lens 5342, a variable filter 5341 and an illumination light source 5343, wherein the illumination lens 5342 is connected with the illumination optical fiber bundle, and the illumination light source is used for providing an illumination light signal;

the imaging module 533 sequentially includes an imaging lens 5331 and a camera 5332, the imaging lens 5331 is connected to the bright field optical fiber bundle, and the camera 5332 is configured to acquire image information of a tissue region to be measured. That is, the endoscope in the three-dimensional nonlinear laser scanning cavity provided in the embodiment of the present invention is further provided with an illumination module 534 and an imaging module 533, wherein the illumination module 534 sequentially includes an illumination lens 5342, a variable optical filter 5341 and an illumination light source 5343, wherein the illumination light source can switch different optical filters through an electric variable optical filter wheel to obtain illumination light signals with different wavelengths, the basic principle is that two-photon fluorescence imaging is not interfered, for example, when autofluorescence and second harmonic are obtained, the illumination light signals can be switched to a red or infrared optical filter to obtain illumination light signals of 370nm, 635nm, infrared 850nm, or 940nm, and the illumination light signals enter an illumination optical fiber bundle through lens coupling;

the imaging module 533 sequentially includes an imaging lens and a camera, the lens being focused on the camera for direct observation of bright field. The two cameras correspond to binocular bright field optical fiber bundles, bright field imaging and two-photon imaging form a multi-mode laparoscope, and bright field binocular three-dimensional laparoscope is in a mode, so that large-view sample observation is carried out, and the basic appearance of a sample is mainly observed. For the suspicious or interested area, the method can be switched to a two-photon mode to perform autofluorescence and second harmonic imaging, and observe the cell grade morphology of the sample, thereby providing a basis for further judgment. Wherein the camera may be an imaging device based on an imaging device such as a CCD or CMOS.

On the basis of the above embodiments, the endoscope in the three-dimensional nonlinear laser scanning cavity provided by the embodiment of the present invention further includes an air extracting device, as shown in fig. 5, the air extracting device 52 mainly includes an air extracting pump connected to the adsorption channel through an air extracting pipeline, an air extracting valve is disposed in the air extracting pipeline, the air extracting valve is electrically connected to the air extracting device 52, the air extracting device 52 controls the air extracting flow of the air extracting pipeline by adjusting the opening and closing of the air extracting valve, so as to realize air extracting control of the adsorption channel, and further adjust the negative pressure in the adsorption channel, so that the endoscope in the cavity is adsorbed on tissues such as intestines, stomach, or oral cavity of a human body through the action of atmospheric pressure, thereby reducing motion artifacts caused by the movement of biological tissues, and making the imaging more.

On the basis of the above embodiments, the endoscope in the three-dimensional nonlinear laser scanning cavity provided by the embodiments of the present invention further includes an industrial personal computer, as shown in fig. 5, the industrial personal computer 532 is electrically connected with a scanning acquisition controller 531, wherein:

the industrial personal computer 532 is configured to obtain the first electrical signal and the second electrical signal acquired by the scanning acquisition controller 531, generate a first fluorescent image based on the first electrical signal, and generate a second fluorescent image based on the second electrical signal. The endoscope in the three-dimensional nonlinear laser scanning cavity provided by the embodiment of the invention further comprises an industrial personal computer 532 which is electrically connected with the scanning acquisition controller 531, the industrial personal computer 532 generates a first fluorescence image based on a first electric signal and a second fluorescence image based on a second electric signal, and the first fluorescence image and the second fluorescence image can be respectively used for displaying information of a cell structure and a fiber structure, wherein the industrial personal computer is provided with control software, and sends a control instruction to the scanner through the control software so as to control the scanning acquisition controller to acquire the first electric signal and the second electric signal.

On the basis of the above embodiments, the endoscope in the three-dimensional nonlinear laser scanning cavity provided by the embodiment of the present invention further includes a display, as shown in fig. 5, the display 55 is electrically connected to the industrial personal computer 532, and is configured to display the first fluorescence image and the second fluorescence image. Namely, the endoscope in the three-dimensional nonlinear laser scanning cavity provided by the embodiment of the invention further comprises a display 55 for displaying the first fluorescence image and the second fluorescence image, and through the display 55, a worker can directly acquire the relevant information of the first fluorescence image and the second fluorescence image.

Fig. 6 is a schematic structural diagram of a second endoscope in a three-dimensional nonlinear laser scanning cavity provided in an embodiment of the present invention, and as shown in fig. 6, the endoscope in the three-dimensional nonlinear laser scanning cavity also includes:

the fluorescence collection device 56, the scanning acquisition controller 531, the femtosecond pulse laser, the fiber coupling module, and the cavity endoscope detection device 1, the air extractor 52, the industrial personal computer 532, the illumination module 534, and the imaging module 533 provided by the above embodiments, wherein the fluorescence collection device 56 and the fiber coupling module are all connected with the cavity endoscope detection device 1 through optical fiber communication, the fluorescence collection device 56 and the cavity endoscope detection device 1 are both electrically connected with the scanning acquisition controller 531, wherein the functions of the above modules or devices are the same as the functions of the devices in the above embodiments, the fluorescence collection device and the cavity endoscope detection device are combined together to form a laser emission module 540, the illumination module 534 sequentially comprises an illumination lens 5342, a variable optical filter 5341 and an illumination light source 5343, the imaging module 533 sequentially comprises an imaging lens and a camera, the cavity endoscope detection device 1 in the three-dimensional nonlinear laser scanning cavity endoscope is a stomatoscope detection device, the optical path structure of the cavity endoscope detection device comprises a liquid lens, the function of the liquid lens is the same as that of the liquid lens in the embodiments, and the optical path is also the same as that of the corresponding optical path in the embodiments.

On the basis of the foregoing embodiments, fig. 7 is a schematic structural diagram of a fluorescence collecting device according to an embodiment of the present invention, and as shown in fig. 7, the fluorescence collecting device according to an embodiment of the present invention includes a collecting fiber-optic universal interface 881, a first photomultiplier 882, a second photomultiplier 883, a first collecting optical path between the collecting fiber-optic universal interface 881 and the first photomultiplier 882, and a second collecting optical path between the collecting fiber-optic universal interface 881 and the second photomultiplier 883, wherein:

the first collecting light path sequentially comprises a coupling collecting lens 81, an infrared filter 82, a first dichroic mirror 83, a first filter 84 and a first collecting lens 85, wherein the first collecting light path is used for collecting the fluorescent signal received by the fluorescent collecting device, and the first photomultiplier 882 is used for converting the fluorescent signal into a first electrical signal;

the second collecting light path sequentially includes a coupling collecting lens 81, an infrared filter 82, a first dichroic mirror 83, a second dichroic mirror 86, a second filter 87, and a second collecting lens 88, wherein the second collecting light path is configured to collect a second harmonic signal received by the fluorescence collecting device, and the second photomultiplier 883 is configured to convert the second harmonic signal into a second electrical signal. That is, the fluorescence collecting device provided in the embodiment of the present invention has a two-way signal collecting function, and integrates two-way optical paths, wherein the first dichroic mirror 83 in the first collecting optical path is a dichroic mirror for transmitting fluorescence signals and reflecting second harmonics, the second dichroic mirror 86 and the first dichroic mirror 83 are the same dichroic mirrors for reflecting second harmonics, the first optical filter 84 is used for transmitting fluorescence signals and filtering out the rest interference signals, the second optical filter 87 is used for transmitting corresponding second harmonic signals and filtering out the rest interference signals, for example, when 780nm femtosecond fiber laser is used to excite autofluorescence in abdominal cavity or oral cavity of human body, 390nm second harmonic signals and 450 and 600nm two-photon autofluorescence signals can be obtained, the wavelengths pass through above 420nm, the dichroic mirror reflected by wavelengths below 420, that is, the first dichroic mirror 83, can separate fluorescence, clean second harmonic signals and fluorescence signals can be obtained by using the first filter 84 of 390 + -20 nm and the second filter 87 of 450-600nm, respectively.

Fig. 8 is a schematic diagram of a box-sealing structure of a box-type combination structure of a three-dimensional nonlinear laser scanning cavity endoscope according to an embodiment of the present invention, as shown in fig. 8, a display 55 integrated on a box cover is integrated with a box body provided with modules, so as to facilitate movement of the whole device and replacement of a work place, and the display 55 can be placed on the box body to facilitate a worker to obtain information on the display when in use, wherein the cavity endoscope detection device 1 in the three-dimensional nonlinear laser scanning cavity endoscope is a mouth endoscope detection device. After the three-dimensional nonlinear laser scanning cavity endoscope is used, a worker can carry the equipment box by hand, so that the working place can be conveniently replaced, and the equipment can be more conveniently used particularly in hospitals, laboratories or outdoor places.

Fig. 9 is a schematic diagram of a second box sealing structure of a box combination structure of a three-dimensional nonlinear laser scanning cavity endoscope according to an embodiment of the present invention, and as shown in fig. 9, a display 55 integrated on a box cover is integrated with a box body provided with modules, so as to facilitate movement of the whole apparatus and replacement of a work place, and when the display 55 is used, the display can be externally placed on the box body to facilitate a worker to obtain information on the display, wherein the cavity endoscope detection device 1 in the three-dimensional nonlinear laser scanning cavity endoscope is a laparoscope detection device, and a plurality of laparoscope detection devices can be simultaneously provided. After the three-dimensional nonlinear laser scanning cavity endoscope is used, a worker can carry the equipment box by hand, so that the working place can be conveniently replaced, and the equipment can be more conveniently used particularly in hospitals, laboratories or outdoor places.

On the basis of the above embodiments, the number of the cavity endoscope detection devices in the three-dimensional nonlinear laser scanning cavity endoscope provided by the embodiments of the present invention is plural. The fluorescence collecting device and the optical fiber coupling module provided by the embodiment of the invention can be simultaneously in optical fiber communication connection with a plurality of cavity endoscope detection devices, namely, a plurality of detection devices are integrated in a three-dimensional nonlinear laser scanning cavity endoscope system so as to realize the simultaneous detection of different parts of gastrointestinal tissues and further carry out contrastive analysis.

On the basis of the above embodiments, the three-dimensional nonlinear laser scanning cavity endoscope provided in the embodiments of the present invention further includes an adjusting optical fiber for optical fiber transmission connection between the fluorescence collecting device and the optical fiber coupling module and the cavity endoscope detecting device, respectively, wherein:

the length of the adjusting optical fiber can be adjusted. The fluorescence collecting device and the optical fiber coupling module in the three-dimensional nonlinear laser scanning cavity endoscope provided by the embodiment of the invention are respectively connected with the cavity endoscope detection device through the adjustable-length adjusting optical fiber in an optical fiber transmission manner, so that the detection device can be flexibly moved according to different experimental scene requirements, the limitation of the limited optical fiber length is avoided, the length of the adjusting optical fiber can be adjusted, and the optical fiber can be replaced at any time in various occasions by replacing optical fibers with different lengths.

As to the three-dimensional nonlinear laser scanning cavity endoscope provided in the above embodiments, the embodiment of the present invention provides another specific implementation manner, fig. 10 is a schematic diagram of a table structure of the three-dimensional nonlinear laser scanning cavity endoscope provided in the embodiment of the present invention, as shown in fig. 10, the three-dimensional nonlinear laser scanning cavity endoscope includes an air pumping device 52, a first device 53, a second device 54, a display 55, and a cavity endoscope detection device 1, wherein the first device 53 integrates a scanning acquisition controller and an industrial personal computer, the industrial personal computer is electrically connected to the display 55, the second device 54 integrates a femtosecond pulse laser, an optical fiber coupling module, a fluorescence collection device, an illumination module, and an imaging module, the optical fiber coupling module and the fluorescence collection device are all in optical fiber transmission connection with an adsorption microscope detection device 51, wherein the cavity endoscope detection device 1 is a mouth endoscope detection device, the absorption type three-dimensional nonlinear laser scanning microscope is used for detecting oral tissue of a human body so as to know information such as benign and malignant tumors, infiltration depth, metastasis conditions, and whether cancer residues exist at cut edges.

Fig. 11 is a schematic diagram of a table structure of a three-dimensional nonlinear laser scanning cavity endoscope, as shown in fig. 11, the three-dimensional nonlinear laser scanning cavity endoscope also includes an air pumping device 52, a first device 53, a second device 54, a display 55 and a cavity endoscope detection device 1, wherein the first device 53 integrates a scanning acquisition controller and an industrial personal computer, the industrial personal computer is electrically connected with the display 55, the second device 54 integrates a femtosecond pulse laser, an optical fiber coupling module, a fluorescence collection device, an illumination module and an imaging module, the optical fiber coupling module and the fluorescence collection device are all in optical fiber transmission connection with an adsorption microscope detection device 51, the cavity endoscope detection device 1 is a laparoscope detection device, and the laparoscope detection device is embedded in the abdomen of a human body to detect gastrointestinal tissues so as to know the quality and malignancy of a tumor, Depth of infiltration, metastasis, and presence or absence of cancer residues at the margin of resection.

Although the present invention has been described with reference to the specific embodiments shown in the drawings, it is not intended to limit the scope of the present invention, and it should be understood by those skilled in the art that the above description is not only a preferred embodiment of the present invention, but also the present invention is not limited thereto, and various changes and modifications may be made by those skilled in the art based on the technical solution of the present invention. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

The above-described embodiments of the apparatus are merely illustrative, and the units described as separate parts may or may not be physically separate, and the parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of the present embodiment. One of ordinary skill in the art can understand and implement it without inventive effort.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims

1. A device for endoscopic detection in a cavity, comprising:

2. The cavity endoscopy apparatus of claim 1, wherein the optical path structure further comprises a liquid lens disposed between the collimating lens and the mems mirror to form a new first optical path, the new first optical path comprising, in order, the collimating lens, the liquid lens, the mems mirror, the lens, the dichroic mirror, and the objective lens disposed between the fiber optic common interface and the port.

3. The cavity endoscope detecting device according to claim 1 or 2, wherein a plurality of illuminating channels are further arranged in the detecting tube, illuminating optical fiber bundles for transmitting illuminating light signals are arranged in the illuminating channels, and the illuminating channels are uniformly distributed by taking the axis of the detecting channel as a center.

4. The borescope probe device of claim 3, further comprising a viewing channel disposed within the probe tube, the viewing channel being located between the probe channel and the illumination channel, wherein:

and an observation lens is arranged at the channel opening of the observation channel and is connected with the bright field optical fiber bundle in the observation channel so as to acquire image information of the tissue area to be detected in front of the objective lens.

5. The borescope probe device of claim 3, further comprising an adsorption channel disposed within the probe tube, the adsorption channel being located between the illumination channel and the probe tube edge.

6. The device as claimed in claim 4, wherein said handle housing defines a button hole, and a switch button is disposed in said button hole for switching different filters to obtain said illumination light signals of different wavelengths.

7. An endoscopic cavity detection device as claimed in claim 6, wherein an imaging button is further disposed in said button hole, said imaging button being configured to control an imaging module connected to said brightfield fiber bundle to image a tissue region to be detected in front of said objective lens.

8. A three-dimensional nonlinear laser scanning cavity endoscope is characterized by comprising:

a fluorescence collection device, a scan acquisition controller, a femtosecond pulse laser, a fiber coupling module, and the intracavity scope detection device of any one of claims 1-7, wherein the fluorescence collection device and the fiber coupling module are both in fiber communication with the intracavity scope detection device, and the fluorescence collection device and the intracavity scope detection device are both electrically connected to the scan acquisition controller, wherein:

9. The three-dimensional nonlinear laser scanning cavity endoscope of claim 8, further comprising an illumination module and an imaging module, both of which are in fiber-optic communication connection with the cavity endoscope detection device, wherein:

the illumination module sequentially comprises an illumination lens, a variable optical filter and an illumination light source, the illumination lens is connected with the illumination optical fiber bundle, and the illumination light source is used for providing an illumination light signal;

the imaging module sequentially comprises an imaging lens and a camera, the imaging lens is connected with the bright field optical fiber bundle, and the camera is used for acquiring image information of a tissue area to be detected.

10. The three-dimensional nonlinear laser scanning cavity endoscope in accordance with claim 8, wherein the fluorescence collecting device comprises a collecting fiber optic universal interface, a first photomultiplier tube, a second photomultiplier tube, and a first collecting light path between the collecting fiber optic universal interface and the first photomultiplier tube, a second collecting light path between the collecting fiber optic universal interface and the second photomultiplier tube, wherein:

the first collecting light path sequentially comprises a coupling collecting lens, an infrared filter, a first dichroic mirror, a first filter and a first collecting lens, wherein the first collecting light path is used for collecting the fluorescent signals received by the fluorescent collecting device, and the first photomultiplier is used for converting the fluorescent signals into first electric signals;

the second collecting light path sequentially comprises the coupling collecting lens, the infrared filter, the first dichroic mirror, the second filter and the second collecting lens, wherein the second collecting light path is used for collecting the second harmonic signals received by the fluorescence collecting device, and the second photomultiplier is used for converting the second harmonic signals into second electric signals.