CN209847125U - Zoom type cavity endoscope detection device and laser scanning cavity endoscope - Google Patents

Abstract

An embodiment of the utility model provides a zoom formula cavity endoscope detection device and laser scanning cavity endoscope. Wherein, above-mentioned formula of zooming cavity endoscope detection device includes handle casing and the detecting tube that reciprocates relatively through first transmission ring and second transmission ring, sets up the first light path including collimating lens, micro-electromechanical scanning mirror, lens, dichroscope, relay lens and objective and the second light path including objective, relay lens and dichroscope in the well siphunculus of handle casing. The embodiment of the utility model provides a cavity endoscope detection device and laser scanning cavity that can zoom passes through reciprocating between the drive ring, drives detecting tube and handle casing and reciprocates relatively, makes the light path structure in the handle casing can zoom the operation, realizes the cell imaging of the different degree of depth such as human abdominal cavity intestines and stomach tissue, oral cavity tissue and palace intracavity tissue, and the infiltration degree of depth, the transfer condition and the surgical operation incisal reason of accurate judgement tumour have whether cancer remain the relevant condition such as.

Description

Zoom type cavity endoscope detection device and laser scanning cavity endoscope

Technical Field

The embodiment of the utility model provides a relate to laser scanning endoscope technical field, especially relate to a formula cavity endoscope detection device zooms and laser scanning cavity endoscope.

Background

Gastrointestinal malignancies are the second leading cause of cancer death in the population of developed countries and this trend has become more evident in recent years. The treatment of gastrointestinal malignant tumor mainly adopts surgical radical resection, but the specific range of the surgical resection needs to be determined when the surgical radical resection is specifically carried out, so before the operation, the benign and malignant tumor, the infiltration depth, the metastasis condition, the existence of cancer residue at the cut edge and the like need to be known. Preoperative gastrointestinal endoscopic biopsy is therefore a very important diagnostic evidence for histological diagnosis of gastrointestinal tumors. The operative form of stomach cancer is divided into full stomach resection, secondary stomach resection, partial gastrectomy, endoscopic mucosal or submucosal resection and the like according to the size, the growth position, the infiltration depth and the like of a tumor body.

Currently, gastrointestinal endoscopic biopsy is usually based on gastrointestinal endoscopy, imaging is performed with the aid of CT, MRI, etc., or a number of gastrointestinal diseases are evaluated with a conventional white light laparoscope or endoscope.

However, there are some inevitable disadvantages in imaging by imaging using CT, MRI, etc. based on enteroscopy, such as easy bleeding of intestinal tube or tumor body in operation, manual pulling or squeezing, delay in time due to repeated endoscopic biopsy when enteroscopy cannot pass through the intestinal tube, and additional emergency hemostasis if severe bleeding is caused. In clinical practice, auxiliary examination means such as CT and MRI cannot accurately judge the infiltration depth of early gastrointestinal tumors and lymph node metastasis. The gastrointestinal tumor T stage is judged by ultrasonic endoscopy, and the accuracy reported in the literature is only 44.7% -78%, which is not enough to become a reliable diagnostic standard. The ultrasonic endoscope also has poor preoperative evaluation effect on the local resection, cannot accurately subdivide gastrointestinal mucosa levels, and has poor N staging effect. Therefore, in view of the current gastrointestinal auxiliary diagnostic technology, a new gastrointestinal tumor diagnostic device is urgently needed to detect the gastrointestinal tissue information at different depths in situ in real time.

SUMMERY OF THE UTILITY MODEL

To the technical problem who exists among the prior art, the embodiment of the utility model provides a zoom formula cavity endoscope detection device and laser scanning cavity endoscope.

In a first aspect, an embodiment of the present invention provides a zoom type cavity endoscope detection device, including:

handle casing and detecting tube, handle casing bottom is provided with well siphunculus and first drive ring, the detecting tube top is provided with second drive ring, first drive ring with second drive ring rotates to be connected, well siphunculus nestification is in the detecting tube and in the first drive ring, be provided with the light path structure that zooms the motor and be used for forming first light path and second light path in the handle casing, wherein:

the zoom motor is used for driving the second transmission ring to move up and down relative to the first transmission ring;

the first optical path sequentially comprises a collimating lens, a micro-electromechanical scanning galvanometer, a lens, a dichroic mirror, a relay lens and an objective lens, wherein the first optical path is used for transmitting a laser signal received by the collimating lens from the collimating lens to the objective lens;

the second optical path sequentially comprises the objective lens, the relay lens and the dichroic mirror, wherein the second optical path is used for conducting the optical signal collected by the objective lens to the dichroic mirror from the objective lens.

In a second aspect, an embodiment of the present invention provides an endoscope in a three-dimensional nonlinear laser scanning cavity, including:

fluorescence collection device, scanning acquisition controller, femto second pulse laser instrument, fiber coupling module and the embodiment of the utility model provides a zoom formula cavity endoscope detection device that the first aspect provided, fluorescence collection device with fiber coupling module all with zoom formula cavity endoscope detection device optical fiber communication connects, fluorescence collection device with zoom formula cavity endoscope detection device all with scanning acquisition controller electricity is connected, wherein:

the femtosecond pulse laser is used for outputting pulse laser signals to the optical fiber coupling module;

the optical fiber coupling module is used for coupling the pulse laser signal output by the femtosecond pulse laser and transmitting the pulse laser signal to the collimating lens in the zoom type cavity endoscope detection device;

the zoom type cavity endoscope detection device is used for receiving the pulse laser signal, outputting the pulse laser signal to an autofluorescent substance in a cell of a living body, acquiring a fluorescence signal and a second harmonic signal generated after the autofluorescent substance is excited through the objective lens, and outputting the fluorescence signal and the second harmonic signal to the fluorescence collection device;

the fluorescence collecting device is used for receiving the fluorescence signal and the second harmonic signal and then respectively converting the fluorescence signal and the second harmonic signal into corresponding electric signals;

and the scanning acquisition controller is used for controlling the micro-electromechanical scanning galvanometer to scan the pulse laser signals and synchronously acquire the electric signals.

The embodiment of the utility model provides a formula of zooming cavity endoscope detection device laser scanning cavity endoscope reciprocates for the first driving ring of handle casing through the second driving ring of the motor drive detecting tube that zooms, in order to realize that the detecting tube reciprocates for the light path structure that the handle casing set up, and then make objective among the light path structure reciprocate for detection device, in order to zoom the operation, realize being in human abdominal cavity internal gastrointestinal tissue, oral cavity tissue and the internal tissue of palace chamber are surveyed time measuring, can carry out the histiocyte formation of image of the different degree of depth, in order to acquire the structural information of the different degree of depth of histiocyte, thereby judge the infiltration degree of depth of tumour more accurately, the condition of transferring and the surgery incisal edge have the relevant circumstances such as no cancer remains, and is simple in operation, high durability and convenient use.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic structural diagram of a zoom type cavity endoscope detection device provided by an embodiment of the present invention;

FIG. 2 is a schematic structural view of a second variable focus cavity endoscope detection device according to an embodiment of the present invention;

fig. 3 is a third schematic structural view of a zoom type cavity endoscope detection device provided by the embodiment of the present invention;

fig. 4 is a schematic structural diagram of a zoom type cavity endoscope detection device provided by the embodiment of the present invention;

fig. 5 is a schematic view of a structure of an endoscope in a three-dimensional nonlinear laser scanning cavity according to an embodiment of the present invention;

fig. 6 is a schematic view of a structure of an endoscope in a three-dimensional nonlinear laser scanning cavity according to an embodiment of the present invention;

fig. 7 is a schematic structural view of a fluorescence collecting device according to an embodiment of the present invention;

fig. 8 is a first box sealing structure diagram of a box combination structure of a three-dimensional nonlinear laser scanning cavity endoscope according to an embodiment of the present invention;

fig. 9 is a second schematic diagram of a box sealing structure of a box combination structure of a three-dimensional nonlinear laser scanning cavity endoscope provided in the embodiment of the present invention;

fig. 10 is a first schematic diagram of a table-type structure of a three-dimensional nonlinear laser scanning cavity endoscope according to an embodiment of the present invention;

fig. 11 is a schematic diagram of a table type structure of a three-dimensional nonlinear laser scanning endoscope.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

At present, the gastrointestinal endoscope is taken as a basis, imaging is carried out by taking CT, MRI and the like as assistance to obtain relevant information of malignancy, infiltration depth, metastasis condition, whether cancer residue exists at incisal margin and the like of tumors, and the method has some defects in specific operation, such as easy bleeding of intestinal tracts or tumor bodies, need of manual drawing or extrusion, delay of time due to repeated endoscopic biopsy when the gastrointestinal endoscope cannot pass through the intestinal tracts, and need of additional emergency hemostasis if severe bleeding is caused. In clinical practice, auxiliary examination means such as CT and MRI cannot accurately judge the infiltration depth of early gastrointestinal tumors and lymph node metastasis. The gastrointestinal tumor T stage is judged by the ultrasonic endoscope, the accuracy reported in the literature is only 44.7% -78%, the judgment is not enough to become a reliable diagnosis standard, the preoperative judgment effect of the ultrasonic endoscope on the local resection operation is poor, the gastrointestinal mucosa levels cannot be accurately subdivided, and the N stage effect is also poor.

While conventional white light laparoscopes and endoscopes can assess many gastrointestinal diseases, the technique is limited to detecting gross morphological changes. Although suspicious regions are easily found, these techniques are associated with false positive rates, specificity, and the like, as compared to in vivo detection techniques. White light endoscopy is associated with wide error in diagnosis of microscopic changes, including examination diagnosis of ulcerative colitis or Barrett's esophagus and flat adenomatous dysplasia. Confocal endoscopy combines laser technology, fluorescence detection technology, rapid scanning technology and the like, and is concerned widely because the confocal endoscope can detect mucosal changes at a microscopic level and can possibly be used for replacing tissue biopsy, and the imaging technology has high sensitivity and specificity. However, the confocal endoscopic imaging technology is still limited by the imaging depth and the fluorescent staining agent, because the gastrointestinal sample has strong absorption and scattering to visible light, the imaging depth is only on the superficial layer, and a specific fluorescent staining developer is required to be injected, the operation is too complicated, and the relevant information such as the infiltration depth of the tumor, the metastasis condition, the existence of cancer residue at the surgical incisal margin and the like cannot be accurately obtained.

The two-photon microscopic imaging technology adopts a femtosecond pulse laser with longer wavelength as an excitation light source, has the characteristics of deep imaging depth, small light damage, small photobleaching area, high fluorescence collection efficiency and the like, and has epoch-making significance in deep imaging of biological tissues. The first two-photon fluorescence microscope in the world was developed by w.denk et al at cornell university in 1990, using a multi-photon microscopic imaging technique based on nonlinear optics and femtosecond pulsed laser. The technology can rapidly obtain the tissue structure and the cell morphology of the specimen in real time by utilizing autofluorescence generated by cells in living tissues and second harmonic generated by collagen tissues. As early as 1986, the second harmonic was used for skin studies and coronary microscopic imaging studies, confirming its feasibility for being used to observe biological tissues. MPM is also an important tool for cancer research. The autofluorescence generated by the cell is derived from Nicotinamide Adenine Dinucleotide (NADH) and Flavin Adenine Dinucleotide (FAD) in the cell, the wavelength emitted by NADH is 460nm, and the second oscillation harmonic of collagen is 370-390 nm, so that a multiphoton microscope with a range of 780-940 nm is usually selected when observing tumor sample tissues. Not only does MPM imaging match standard tumor histopathology, it also provides additional information about the tumor neogenesis process, as can the metabolic levels of tumor tissue cells by measuring the ratio NADH/FAD.

With multiphoton imaging techniques, multiphoton microscopy is capable of providing real-time gastrointestinal tract tissue structure and cell morphology information. The multi-photon imaging technology has the characteristics of no need of exogenous labeled tissues, extremely sensitivity to collagen, small photodamage to tissues, deep penetration depth and the like, and can be possibly applied to optical biopsy of gastrointestinal tumors. There are no clinically available two-photon laparoscopes and endoscopes, and no two-photon imaging-based intracavity scope detection device to detect gastrointestinal tract tissue information in situ and in real time.

For the different degree of depth's of normal position real-time detection intestines and stomach tissue information, the embodiment of the utility model provides a formula cavity endoscope detection device zooms, figure 1 does the embodiment of the utility model provides a formula cavity endoscope detection device zooms structure schematic diagram one, as shown in figure 1, this formula cavity endoscope detection device zooms includes:

handle casing 11 and detecting tube 12, handle casing 11 bottom is provided with well siphunculus 13 and first drive ring 111, and detecting tube 12 top is provided with second drive ring 121, and first drive ring 111 rotates with second drive ring 121 to be connected, and well siphunculus 13 nestification is provided with the light path structure that zooms motor 119 and be used for forming first light path and second light path in detecting tube 12 and first drive ring 111 in, handle casing 11, wherein:

a zoom motor 119 for driving the second driving ring 121 to move up and down with respect to the first driving ring 111;

the first optical path sequentially comprises a collimating lens 113, a micro-electromechanical scanning galvanometer 114, a lens 115, a dichroic mirror 116, a relay lens 117 and an objective lens 118, wherein the first optical path is used for conducting a laser signal received by the collimating lens 113 from the collimating lens 113 to the objective lens 118;

the second optical path includes an objective lens 118, a relay lens 117, and a dichroic mirror 116 in sequence, wherein the second optical path is used for guiding the optical signal collected by the objective lens 118 from the objective lens 118 to the dichroic mirror 116.

Specifically, the embodiment of the present invention provides a zoom type cavity endoscope detection device, which comprises two main structures, namely a handle housing 11 and a detection tube 12, wherein the handle housing 11 has a cavity therein, an opening is formed at the bottom end of the housing, a through tube 13 is disposed at the opening, a light path structure forming a first light path and a second light path is formed in the handle housing 11, the first light path comprises a collimating lens 113, a micro-electromechanical scanning galvanometer 114, a lens 115, a dichroic mirror 116, a relay mirror 117 and an objective lens 118, and is used for exciting a laser signal of a spontaneous fluorescent substance in gastrointestinal tissue or oral tissue cells of a human body, the laser signal passes through the collimating lens 113, the micro-electromechanical scanning galvanometer 114, the lens 115, the dichroic mirror 116, the relay mirror 117 and the objective lens 118 in the first light path, and exits from the objective lens 118 to the spontaneous fluorescent substance, after exciting the spontaneous fluorescent, two-photon signals and second harmonic signals are collected through an objective lens 118, and the two-photon signals and the second harmonic signals pass through a relay lens 117 and a dichroic mirror 116 in a second light path, and are collected into a fluorescence collecting device to obtain detection information of gastrointestinal tissues or oral tissues to be detected so as to judge related information such as tumor infiltration depth, metastasis condition, and whether cancer residues exist at the surgical cutting edge, wherein a first transmission ring 111 is arranged outside an opening at the bottom end of a handle shell 11, a middle through pipe 13 is nested in the first transmission ring 111, a second transmission ring 121 at the top end of a detection pipe 12 is connected with the first transmission ring 111 in a relatively rotating fit manner, a zoom motor 119 arranged in the handle shell 11 drives the second transmission ring 121 and the first transmission ring 111 to move up and down relatively, including a relative rotating manner, and directly driven to vertically move up and down, so that the detection pipe 12 includes a light path structure relative to, move up and down, so that the whole optical path structure can move up and down relative to the channel port of the detection channel in the detection tube 12, tissue cells at different depths are detected, and cell structure information at different depths is acquired.

The relay lens 117 is arranged inside the objective lens 118 and used for conducting a laser signal for exciting the spontaneous fluorescent material from the dichroic mirror 116 to the objective lens 118 in a long distance, conducting a two-photon signal and a second harmonic signal collected by the objective lens 118 to the dichroic mirror 116, wherein the image plane of the laser signal objective lens 118 is overlapped with the focal plane of the relay lens 117, and conducting a laser signal scanning area passing through the micro-electromechanical scanning lens to the image plane of the objective lens 118 in an equal ratio of 1:1, and the relay lens 117 can be lengthened or shortened according to specific needs.

The dichroic mirror 116 may be set as a long-pass short-dichroic mirror 116 or a short-pass long-dichroic mirror 116 as required, that is, when the long-pass short-dichroic mirror 116 is set, a pulse laser signal for exciting a spontaneous fluorescent substance is transmitted, and a collected two-photon signal and a second harmonic signal are reflected, as shown in fig. 1, at this time, the zoom type cavity endoscope detection device may be a detection device of a laparoscope or a hysteroscope; fig. 2 is the utility model provides a zoom formula cavity endoscope detection device structure sketch map two, as shown in fig. 2, when this dichroic mirror 116 is short-pass long reverse dichroic mirror 116, the reflection is used for arousing autofluorescence's pulse laser signal, two photon signal and second harmonic signal that the transmission was collected, this dichroic mirror 116 reflects through collimating lens 113, micro-electromechanical scanning mirror 114 that shakes, the laser signal that incides on dichroic mirror 116 behind lens 115 passes through relay lens 117 to objective 118, two photon signal and second harmonic signal that transmission objective 118 gathered, at this moment, this zoom formula cavity endoscope detection device can be the detection device of mouth mirror.

The embodiment of the utility model provides a formula of zooming cavity endoscope detection device reciprocates for handle shell's first drive ring through the second drive ring of the motor drive probe tube that zooms, in order to realize that the probe tube reciprocates for handle shell includes the light path structure that sets up in the handle shell, and then make objective among the light path structure reciprocate for detection device, in order to zoom the operation, realize being in stomach and intestine tissue in human abdominal cavity, oral cavity tissue and palace intracavity tissue are surveyed time measuring, can carry out the histiocyte imaging of the different degree of depth, in order to acquire the structural information of the different degree of depth of histiocyte, thereby judge the infiltration degree of depth of tumour more accurately, it has the relevant circumstances such as no cancer remains to shift the condition and surgery incisal reason, and is easy operation, high durability and convenient use.

On the basis of each embodiment, the embodiment of the utility model provides a zoom and be provided with the detection passageway in the detecting tube among the formula cavity endoscope detecting device, be provided with light path passageway in the well siphunculus, well siphunculus nestification is in detecting the passageway, detects the passway department of passageway and light path passageway's passway parallel and level, and the passway department of detecting the passageway is provided with the cover glass, wherein:

the collimating lens, the micro-electro-mechanical scanning galvanometer, the lens, the dichroic mirror, the relay lens and the objective lens in the light path structure are all positioned between the optical fiber general interface of the handle shell and the channel port of the light path channel;

the objective lens and the relay lens are both arranged in the light path channel, and the objective lens is positioned at the channel opening of the light path channel. That is, the middle through pipe in the zoom type cavity endoscope detection device provided by the embodiment of the present invention is not only nested in the first transmission ring, but also nested in the detection channel and the second transmission ring of the detection device, the middle through pipe is provided with a light path channel, the light path structure for forming the first light path and the second light path is located between the optical fiber general interface in the handle casing and the passage port of the light path channel, the optical fiber general interface is externally connected with various transmission optical fibers, wherein, the objective lens and the relay lens shared by the first light path and the second light path are both arranged in the light path channel, because the passage port of the detection channel is flush with the passage port of the light path channel, the objective lens at the passage port of the light path channel is also located at the passage port of the detection channel, thereby when the detection pipe moves up and down relative to the handle casing, the objective lens arranged at the passage port of the light path channel can, so with according to the experimental needs, adjustable objective and the distance between the cover glass to carry out the histiocyte formation of image of the different degree of depth, obtain the structural information of the different degree of depth of histiocyte, thereby judge more accurately that the infiltration degree of depth, the transfer condition and the surgery operation incisal margin of tumour have relevant condition such as cancer residue, easy operation, convenient to use.

On the basis of each embodiment, the embodiment of the utility model provides an optical path structure among the formula of zooming cavity endoscope detection device still includes liquid lens, and fig. 3 is the utility model provides a formula of zooming cavity endoscope detection device structure schematic diagram is three, as shown in fig. 3, liquid lens 110 is located collimating lens 113 and the micro-electromechanical scanning mirror 114 that shakes to form new first optical path, new first optical path includes collimating lens 113, liquid lens 110, the micro-electromechanical scanning mirror 114 that shakes, lens 115, dichroic mirror and objective 118 in proper order. That is, the liquid lens 110 is disposed such that the surface of the liquid lens 110 can be correspondingly curved by applying a voltage or a current to the liquid lens 110, and thus parallel light emitted from the collimating lens 113 can be aligned to generate different powers. The specific light path is as follows: laser signals are emitted from the optical fibers, parallelly enter the liquid lens 110 after passing through the collimating lens 113, corresponding focal power is generated from the liquid lens 110 according to loaded voltage or current signals, and emitted convergent or divergent light passes through the micro-electro-mechanical scanning galvanometer 114, the lens and the dichroic mirror, is transmitted to the objective lens 118 through the relay lens 117, and is converged on a sample. The focal power change introduced by the liquid lens 110 can make the focal point of the laser signal emitted from the port of the objective lens 118 move back and forth in the depth direction, and the response speed of the liquid lens 110 is very fast, and the scanning frequency is in the order of KHz, so that fast depth direction scanning imaging can be realized. The liquid lens 110 is equivalent to parallel plate glass when no voltage or current signal is applied, and has no focal power to the laser signal and does not cause any offset of the focus behind the objective lens 118, thereby realizing three-dimensional stereo imaging. In specific use, the liquid lens 110 is complementary to the zoom motor 119, the position of the objective lens 118 is adjusted through the zoom motor 119, after coarse adjustment to the corresponding depth position, the system is switched to a liquid lens 110 zoom scanning mode to perform rapid three-dimensional imaging on a sample, wherein when the zoom type cavity endoscope detection device is not provided with the zoom motor 119, the zoom adjustment can be performed only through the liquid lens 110.

On the basis of each embodiment, the embodiment of the utility model provides a still be provided with a plurality of illumination passageways in the detection pipe among the formula of zooming cavity endoscope detection device, fig. 4 is the utility model provides a formula of zooming cavity endoscope detection device structure schematic diagram is four, as shown in fig. 4, is provided with the illumination fiber bundle that is used for transmitting illumination optical signal in the illumination passageway 123, and wherein illumination passageway 123 uses the axle center of detecting the passageway as central evenly distributed. That is to say, the embodiment of the utility model provides a zoom in detection tube among formula cavity endoscope detection device still is provided with a plurality of illumination passageways 123, this illumination passageway 123 is more than one, all be provided with the illumination fiber bundle in every passageway, illumination fiber has certain aperture angle, do not need lens can directly be used for dispersing the illumination, and illumination passageway 123 uses the axle center of detecting the passageway as central evenly distributed, provide even illumination for zooming formula cavity endoscope detection device to tissue region state that awaits measuring before convenient work observation objective.

On the basis of each embodiment, the embodiment of the utility model provides a zoom formula cavity endoscope detection device in still be provided with in the detecting tube and observe the passageway, as shown in fig. 4, observe the passageway and be located between detecting channel and the illumination passageway, wherein:

an observation lens 122 is arranged at the channel opening of the observation channel, and the observation lens 122 is connected with the bright field optical fiber bundle in the observation channel and used for acquiring image information of a tissue area to be detected in front of the objective lens. That is to say the embodiment of the utility model provides a zoom formula cavity endoscope detection device in the detecting tube in still be provided with the observation passageway, should observe the passageway and be located between detection passageway and the illumination channel, and be provided with observation camera lens 122 and bright field fiber bundle, bright field fiber bundle is imaging fiber bundle promptly for the regional image information of the tissue that awaits measuring before the objective that transmission observation camera lens 122 caught, wherein observe the passageway can be one, also can be for two formation two mesh observations, realize three-dimensional bright field cavity endoscope function.

On the basis of the above embodiments, the embodiment of the present invention provides a zoom type cavity endoscope detection device, wherein an absorption channel is further disposed in the detection tube, and as shown in fig. 4, the absorption channel 124 is located between the illumination channel and the edge of the detection tube. That is to say, the embodiment of the utility model provides a zoom in detection tube among the formula cavity endoscope detection device still be provided with in being used for making zoom in the formula cavity endoscope detection device adsorb the absorption passageway 124 on the tissue that awaits measuring, adsorb the air in the passageway 124 through the extraction, form the negative pressure in adsorbing the passageway 124, make zoom in formula cavity endoscope detection device adsorb on the tissue that awaits measuring, wherein, adsorb passageway 124 and be located between illumination passageway and the detecting tube edge, be located the illumination passageway outside promptly, be close to the position department of detecting tube avris.

On the basis of the above embodiments, the embodiment of the utility model provides a button hole is opened on the handle casing in the zoom type cavity endoscope detection device, a switching button and an imaging button are arranged in the button hole, the switching button is used for switching different optical filters to obtain the illumination light signals with different wavelengths;

the imaging button is used for controlling an imaging module connected with the bright field optical fiber bundle to image a tissue area to be detected in front of the objective lens. Namely, the switching button is arranged in the button hole of the handle shell in the zoom type cavity endoscope detection device provided by the embodiment of the utility model, and the optical filters for filtering the illumination light signals with different wavelengths can be switched through the switching button, so that the workers can select the illumination light signals with different wavelengths; an imaging button is arranged in a button hole of a handle shell in the zoom type cavity endoscope detection device, an imaging module connected with a brightfield optical fiber bundle can be controlled to photograph and image a tissue area to be detected in front of an objective lens through the imaging button, and the functions of the switching button and the imaging button can be customized through software to modify the corresponding functions.

The embodiment of the utility model provides a still provide a three-dimensional nonlinear laser scanning cavity inside speculum, fig. 5 does the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity inside speculum structure sketch map one, as shown in fig. 5, this three-dimensional nonlinear laser scanning cavity inside speculum includes:

fluorescence collection device 56, scanning acquisition controller 531, femto second pulse laser instrument, optical fiber coupling module and the formula of zooming cavity endoscope detecting device 1 that above-mentioned each embodiment provided, fluorescence collection device 56 and optical fiber coupling module all with zoom formula cavity endoscope detecting device 1 optical fiber communication connection, fluorescence collection device and zoom formula cavity endoscope detecting device all with scanning acquisition controller 531 electricity be connected, wherein:

the femtosecond pulse laser is used for outputting pulse laser signals to the optical fiber coupling module;

the optical fiber coupling module is used for coupling a pulse laser signal output by the femtosecond pulse laser and transmitting the pulse laser signal to a collimating lens in the zoom type cavity endoscope detection device 1;

the zoom type cavity endoscope detection device 1 is used for receiving the pulse laser signal, outputting the pulse laser signal to the autofluorescence substance in the living body cell, acquiring a fluorescence signal and a second harmonic signal generated after the autofluorescence substance is excited through the objective lens, and outputting the fluorescence signal and the second harmonic signal to the fluorescence collection device 56;

a fluorescence collecting device 56 for receiving the fluorescence signal and the second harmonic signal and converting the fluorescence signal and the second harmonic signal into corresponding electrical signals;

and the scanning acquisition controller 531 is used for controlling the micro-electromechanical scanning galvanometer to scan the pulse laser signals and synchronously acquire electric signals.

Specifically, the embodiment of the present invention provides a three-dimensional nonlinear laser scanning cavity endoscope, which comprises a fluorescence collecting device 56, a scanning collecting controller 531, a femtosecond pulse laser, a fiber coupling module and a zoom cavity endoscope detecting device 1, thereby forming a three-dimensional nonlinear laser scanning cavity endoscope for detecting gastrointestinal tissues and oral tissues by using a two-photon imaging technique, wherein the femtosecond pulse laser can emit pulse laser signals for exciting autofluorescence in gastrointestinal tissues and oral tissue cells of a human body, generating multiphoton fluorescence signals and second harmonic signals, including using the 920nm femtosecond pulse laser to excite FAD and collagen in cells, exciting 500-600nm fluorescence signals and 460nm second harmonic signals, and exciting autofluorescence such as FAD or NADH in cells by 780nm femtosecond pulse laser, to generate corresponding fluorescence signal and second harmonic signal, wherein the femtosecond pulse laser and the fiber coupling module are combined together to form a laser emission module 540;

the fluorescence collecting device 56 integrates two signal collecting optical paths, namely a fluorescence signal collecting optical path and a second harmonic signal collecting optical path, to respectively collect the fluorescence signal and the second harmonic signal; the scanning acquisition controller 531 controls the micro-electromechanical scanning galvanometer to scan the pulse laser signal and excite the autofluorescence substance to generate a fluorescence signal and a second harmonic signal, and acquires a first electric signal and a second electric signal obtained by converting the fluorescence signal and the second harmonic signal by the fluorescence collection device; the three-dimensional nonlinear laser scanning cavity endoscope can be divided into a laparoscope and a mouth mirror according to different structures of the zoom type cavity endoscope detection device 1. The resolution of the three-dimensional nonlinear laser scanning cavity endoscope can be set to 800nm, the imaging field of view can be 400 micrometers by 400 micrometers, and the imaging speed can be 26 frames (256 pixels by 256) or 13 frames (512 pixels by 512 pixels).

The embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope adopts fluorescence collection device, scanning acquisition controller, femto second pulse laser, optic fibre coupling module and zooming formula cavity endoscope detection device, thereby form the laser scanning cavity endoscope that utilizes two-photon imaging technique to survey human intestines and stomach tissue and oral cavity tissue, adjust objective focal length through liquid lens and zoom motor, realize that laser scanning microscope carries out three-dimensional scanning to the cell structure of different degree of depth, arouse intracellular fluorescent substance spontaneous acquisition multiphoton fluorescence signal and second harmonic signal through femto second pulse laser, realize laser scanning microscope nonlinearity, collect fluorescence signal and second harmonic signal through fluorescence collection device, and convert corresponding electric signal, and then obtain corresponding fluorescence image etc. that reflects cell tissue structure through this electric signal, wherein, the adoption of the zoom type cavity endoscope detection device can lead the staff to be flexible to detect the gastrointestinal tissues, the oral tissues and the tissues in the uterine cavity in the abdominal cavity of the human body, when the gastrointestinal tissues of the human body are detected, only a small opening is required to be formed in the abdomen of the human body, so that the operation cost and the pain of a patient are reduced, when the tissues in the uterine cavity are imaged, noninvasive detection can be carried out through a natural channel (vagina), the equipment is simple to operate and convenient to use.

On the basis of each embodiment, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope still includes lighting module and imaging module, as shown in fig. 5, lighting module 534 and imaging module 533 all with zoom formula cavity endoscope detection device fiber communication connection, wherein:

the illumination module 534 sequentially comprises an illumination lens 5342, a variable filter 5341 and an illumination light source 5343, wherein the illumination lens 5342 is connected with the illumination optical fiber bundle, and the illumination light source is used for providing an illumination light signal;

the imaging module 533 sequentially includes an imaging lens 5331 and a camera 5332, the imaging lens 5331 is connected to the bright field optical fiber bundle, and the camera 5332 is configured to acquire image information of a tissue region to be measured. That is to say, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope still is provided with illumination module 534 and imaging module 533, wherein, illumination module 534 includes illuminating lens 5342, variable optical filter 5341 and illuminating light source 5343 in proper order, wherein, illuminating light source can switch different optical filters through electronic variable optical filter runner, in order to obtain the illuminating light signal of different wavelengths, the fundamental principle is that two-photon fluorescence imaging is not disturbed, for example when obtaining autofluorescence and second harmonic, can switch to red or infrared optical filter 635, in order to obtain 370nm, illuminating light nm or infrared 850nm, the illuminating light signal of 940nm, illuminating light signal gets into illumination fiber bundle through the lens coupling;

the imaging module 533 sequentially includes an imaging lens and a camera, the lens being focused on the camera for direct observation of bright field. The two cameras correspond to binocular bright field optical fiber bundles, bright field imaging and two-photon imaging form a multi-mode laparoscope, and bright field binocular three-dimensional laparoscope is in a mode, so that large-view sample observation is carried out, and the basic appearance of a sample is mainly observed. For the suspicious or interested area, the method can be switched to a two-photon mode to perform autofluorescence and second harmonic imaging, observe the cell-level morphology of the sample and provide a basis for further judgment, wherein the camera can be imaging equipment based on imaging devices such as CCD or CMOS.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope, still include air exhaust device, as shown in fig. 5, air exhaust device 52 mainly includes the aspiration pump, link to each other with the absorption passageway through the exhaust pipe, set up the bleeder valve in the exhaust pipe, the bleeder valve is connected with air exhaust device 52 electricity, air exhaust device 52 is through the switch of adjustment bleeder valve and the size of switching, the bleed-off flow of control exhaust pipe, thereby realize the control of bleeding to the absorption passageway, and then adjust the negative pressure in the absorption passageway, make zooming formula cavity endoscope detection device pass through the effect of atmospheric pressure, adsorb on human abdominal cavity intestines and stomach, on tissues such as oral cavity and palace chamber, reduce the motion artifact that the organism tissue activity brought, make the formation of image more stable, it is clear.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope still includes the industrial computer, as shown in fig. 5, industrial computer 532 is connected with scanning acquisition controller 531 electricity, wherein:

the industrial personal computer 532 is configured to obtain the first electrical signal and the second electrical signal acquired by the scanning acquisition controller 531, generate a first fluorescent image based on the first electrical signal, and generate a second fluorescent image based on the second electrical signal. Namely the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope still includes the industrial computer 532 with scan acquisition controller 531 electricity is connected, this industrial computer 532 is based on first signal of telecommunication generation first fluorescence image and based on the second signal of telecommunication generation second fluorescence image, can be used for showing cell structure and fibrous structure information respectively, wherein installs control software on the industrial computer, through control software, sends control command to the scanner to control scan acquisition controller, acquire above-mentioned first signal of telecommunication and second signal of telecommunication.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope still includes the display, as shown in fig. 5, display 55 is connected with industrial computer 532 electricity for show first fluorescence image and second fluorescence image. Namely, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope is still including being used for showing display 55 of first fluorescence image and second fluorescence image, through display 55, the staff can directly acquire the relevant information of first fluorescence image and second fluorescence image.

Wherein, fig. 6 is the utility model discloses an embodiment provides a speculum structure sketch map two in three-dimensional nonlinear laser scanning cavity, as shown in fig. 6, this speculum in three-dimensional nonlinear laser scanning cavity also includes:

the fluorescence collection device 56, the scanning collection controller 531, the femtosecond pulse laser, the optical fiber coupling module, and the zoom type cavity endoscope detection device 1, the air extractor 52, the industrial personal computer 532, the illumination module 534, and the imaging module 533 provided by the above embodiments, wherein the fluorescence collection device 56 and the optical fiber coupling module are both in optical fiber communication connection with the zoom type cavity endoscope detection device 1, the fluorescence collection device 56 and the zoom type cavity endoscope detection device 1 are both electrically connected with the scanning collection controller 531, wherein the functions of the above modules or devices are the same as the functions of the devices in the above embodiments, the modules or devices include the femtosecond pulse laser and the optical fiber coupling module which are combined together to form the laser emission module 540, the illumination module 534 sequentially includes the illumination lens 5342, the variable optical filter 5341, and the illumination light source 5343, and the imaging module 533 sequentially includes the imaging lens and the camera, the zoom type cavity endoscope detection device 1 in the three-dimensional nonlinear laser scanning cavity endoscope is a mouth endoscope detection device, a light path structure of the zoom type cavity endoscope detection device comprises a liquid lens, the function of the liquid lens is the same as that of the liquid lens in each embodiment, and the light path is also the same as that of the corresponding light path in each embodiment.

On the basis of the foregoing embodiments, fig. 7 is a structural schematic diagram of the fluorescence collecting device provided by the embodiment of the present invention, as shown in fig. 7, the embodiment of the present invention provides a fluorescence collecting device including collecting the general optical fiber interface 881, the first photomultiplier 882, the second photomultiplier 883, and the first collecting optical path between the collecting general optical fiber interface 881 and the first photomultiplier 882, and the second collecting optical path between the collecting general optical fiber interface 881 and the second photomultiplier 883, wherein:

the first collecting light path sequentially comprises a coupling lens 81, an infrared filter 82, a first dichroic mirror 83, a first filter 84 and a first collecting lens 85, wherein the first collecting light path is used for collecting the fluorescent signal received by the fluorescent collecting device, and the first photomultiplier 882 is used for converting the fluorescent signal into a first electrical signal;

the second collecting light path sequentially includes a coupling lens 81, an infrared filter 82, a first dichroic mirror 83, a second dichroic mirror 86, a second filter 87, and a second collecting lens 88, wherein the second collecting light path is configured to collect a second harmonic signal received by the fluorescence collecting device, and the second photomultiplier 883 is configured to convert the second harmonic signal into a second electrical signal. That is, the fluorescence collecting device provided by the embodiment of the present invention has a two-way signal collecting function, and integrates two light paths, wherein the first dichroic mirror 83 in the first collecting light path is a transmission fluorescence signal, the dichroic mirror for reflecting the second harmonic, the second dichroic mirror 86 and the first dichroic mirror 83 are the same dichroic mirror, and are used for reflecting the second harmonic, the first optical filter 84 is used for transmitting the fluorescence signal, and filtering the rest interference signals, and the second optical filter 87 is used for transmitting the corresponding second harmonic signal, and filtering the rest interference signals, for example, when using 780nm femtosecond fiber laser to excite the autofluorescence substance in the human abdominal cavity or oral cavity cell, 390nm second harmonic signal and 450 and 600nm two-photon autofluorescence signal can be obtained, the wavelength passes through above 420nm, the dichroic mirror with wavelength reflection below 420, that is, the first dichroic mirror 83, can separate two paths of fluorescence, clean second harmonic signals and fluorescence signals can be obtained by using the first filter 84 of 390 + -20 nm and the second filter 87 of 450-600nm, respectively.

Wherein, fig. 8 is the utility model provides a box integrated configuration's of three-dimensional nonlinear laser scanning cavity endoscope joint sealing structure sketch map one, as shown in fig. 8, display 55 is integrated together with the box integration of installing each module on the case lid, make things convenient for whole equipment to remove, and change the workplace, and this display 55 when using, can place outward on the box, in order to make things convenient for the staff to acquire the information on the display, wherein speculum detecting device 1 is oral cavity mirror detecting device in the zoom formula cavity among this three-dimensional nonlinear laser scanning cavity endoscope. After the three-dimensional nonlinear laser scanning cavity endoscope is used, a worker can carry the equipment box by hand, so that the working place can be conveniently replaced, and the equipment can be more conveniently used particularly in hospitals, laboratories or outdoor places.

Wherein, fig. 9 is the utility model provides a box integrated configuration's of three-dimensional nonlinear laser scanning cavity endoscope joint sealing structure sketch map two, as shown in fig. 9, display 55 is integrated together with the box integration of installing each module on the case lid, make things convenient for whole equipment to remove, and change workplace, and this display 55 when using, can place outward on the box, in order to make things convenient for the staff to acquire the information on the display, wherein detection device 1 is peritoneoscope detection device in the zoom formula cavity in this three-dimensional nonlinear laser scanning cavity endoscope, and peritoneoscope detection device can set up a plurality ofly simultaneously. After the three-dimensional nonlinear laser scanning cavity endoscope is used, a worker can carry the equipment box by hand, so that the working place can be conveniently replaced, and the equipment can be more conveniently used particularly in hospitals, laboratories or outdoor places.

On the basis of above-mentioned each embodiment, the utility model provides a zoom formula cavity endoscope detection device in three-dimensional nonlinear laser scanning cavity endoscope is a plurality of. Namely the embodiment of the utility model provides a fluorescence collection device and fiber coupling module can be simultaneously with a plurality of formula cavity endoscope detection device optical fiber communication connection that zoom, a plurality of detection device of integration in a three-dimensional nonlinear laser scanning cavity endoscope system promptly to the realization is surveyed the different positions of intestines and stomach tissue simultaneously, thereby carries out contrastive analysis.

On the basis of each embodiment, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope still is including adjusting optic fibre for fluorescence collection device and optical fiber coupling module respectively with zoom formula cavity endoscope detection device between the optic fibre transmission be connected, wherein:

the length of the adjusting optical fiber can be adjusted. Namely the embodiment of the utility model provides a fluorescence collection device and fiber coupling module in three-dimensional nonlinear laser scanning cavity endoscope carry out optical fiber transmission through adjustable length's regulation optic fibre and formula cavity endoscope detecting device that zooms respectively and are connected, in order to realize according to different experiment scene needs, carry out nimble detection device that removes, avoid limited fiber length's restriction, wherein, the length adjustable of adjusting optic fibre, for the optic fibre through changing different length, realize the application of various occasions, can carry out the optic fibre change of different length as required at any time.

To the three-dimensional nonlinear laser scanning cavity endoscope provided by the above embodiments, the embodiment of the present invention provides another specific implementation manner, fig. 10 is a schematic diagram of a table structure of the three-dimensional nonlinear laser scanning cavity endoscope provided by the embodiment of the present invention, as shown in fig. 10, the three-dimensional nonlinear laser scanning cavity endoscope includes an air extractor 52, a first device 53, a second device 54, a display 55 and a zoom type cavity endoscope detection device 1, wherein the first device 53 integrates a scanning acquisition controller and an industrial personal computer, the industrial personal computer is electrically connected with the display 55, the second device 54 integrates a femtosecond pulse laser, an optical fiber coupling module and a fluorescence collection device, an illumination module and an imaging module, the optical fiber coupling module and the fluorescence collection device are all connected with an absorption type microscope detection device 51 through optical fiber transmission, the zoom type cavity endoscope detection device 1 is a mouth cavity endoscope detection device, and is used for detecting oral tissues of a human body so as to know information such as benign and malignant tumors, infiltration depth, metastasis conditions, and whether cancer residues exist at incisional edges.

Wherein, fig. 11 is a schematic diagram of a table structure of a three-dimensional nonlinear laser scanning cavity endoscope, as shown in fig. 11, the three-dimensional nonlinear laser scanning cavity endoscope also includes an air extractor 52, a first device 53, a second device 54, a display 55 and a zoom type cavity endoscope detection device 1, wherein, the first device 53 integrates a scanning acquisition controller and an industrial control computer, the industrial control computer is electrically connected with the display 55, the second device 54 integrates a femtosecond pulse laser, an optical fiber coupling module and a fluorescence collection device, an illumination module and an imaging module, the optical fiber coupling module and the fluorescence collection device are both in optical fiber transmission connection with an adsorption type microscope detection device 51, wherein, the zoom type cavity endoscope detection device 1 is a laparoscope detection device, the laparoscope detection device is embedded into the abdomen of a human body to detect gastrointestinal tissues, in addition, the laparoscope based on the laparoscope detection device can also be used for detecting the tissues in the uterine cavity of a woman, and the imaging principle of the detected tissues is the same as that of the cavity endoscope of each embodiment.

Although the present invention has been described with reference to the accompanying drawings, it is not intended to limit the scope of the invention, and it should be understood by those skilled in the art that the above is not required to be limited to the preferred embodiments of the present invention, but rather, the present invention is not limited to the above embodiments. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

The above-described embodiments of the apparatus are merely illustrative, and the units described as separate parts may or may not be physically separate, and the parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of the present embodiment. One of ordinary skill in the art can understand and implement it without inventive effort.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.

Claims (10)

1. A variable focus cavity endoscopic probe apparatus, comprising:

handle casing and detecting tube, handle casing bottom is provided with well siphunculus and first drive ring, the detecting tube top is provided with second drive ring, first drive ring with second drive ring rotates to be connected, well siphunculus nestification is in the detecting tube and in the first drive ring, be provided with the light path structure that zooms the motor and be used for forming first light path and second light path in the handle casing, wherein:

the zoom motor is used for driving the second transmission ring to move up and down relative to the first transmission ring;

the first optical path sequentially comprises a collimating lens, a micro-electromechanical scanning galvanometer, a lens, a dichroic mirror, a relay lens and an objective lens, wherein the first optical path is used for transmitting a laser signal received by the collimating lens from the collimating lens to the objective lens;

the second optical path sequentially comprises the objective lens, the relay lens and the dichroic mirror, wherein the second optical path is used for conducting the optical signal collected by the objective lens to the dichroic mirror from the objective lens.

2. The zoom type cavity endoscope detection device according to claim 1, wherein a detection channel is arranged in the detection tube, a light path channel is arranged in the middle through tube, the middle through tube is nested in the detection channel, a channel port of the detection channel is flush with a channel port of the light path channel, and a cover glass is arranged at the channel port of the detection channel, wherein:

the collimating lens, the micro-electromechanical scanning galvanometer, the lens, the dichroic mirror, the relay lens and the objective lens in the optical path structure are all positioned between the optical fiber general interface of the handle shell and the channel port of the optical path channel;

the objective lens and the relay lens are arranged in the light path channel, and the objective lens is positioned at the position of a channel opening of the light path channel.

3. An endoscopic detection apparatus in a zoom cavity according to claim 1 or 2, wherein the optical path structure further comprises a liquid lens, the liquid lens is located between the collimating lens and the micro-electromechanical scanning galvanometer to form a new first optical path, and the new first optical path sequentially comprises the collimating lens, the liquid lens, the micro-electromechanical scanning galvanometer, the lens, the dichroic mirror, and the objective lens.

4. The variable focus cavity endoscope detection device according to claim 2, wherein a plurality of illumination channels are further arranged in the detection tube, and illumination fiber bundles for transmitting illumination light signals are arranged in the illumination channels, wherein the illumination channels are uniformly distributed around the axis of the detection channel.

5. The variable focus cavity endoscopic detection apparatus according to claim 4, wherein an observation channel is further disposed within said detection tube, said observation channel being located between said detection channel and said illumination channel, wherein:

and an observation lens is arranged at the channel opening of the observation channel and is connected with the bright field optical fiber bundle in the observation channel so as to acquire image information of the tissue area to be detected in front of the objective lens.

6. The variable focus cavity endoscopic detection apparatus according to claim 4, wherein an absorption channel is further disposed in the detection tube, and the absorption channel is located between the illumination channel and the detection tube edge.

7. The variable focus cavity endoscope detection device according to claim 5, wherein said handle housing has a button hole, and a switch button and an imaging button are disposed in said button hole, and said switch button is used for switching different filters to obtain said illumination light signals with different wavelengths;

the imaging button is used for controlling an imaging module connected with the bright field optical fiber bundle to image a tissue area to be detected in front of the objective lens.

8. A three-dimensional nonlinear laser scanning cavity endoscope is characterized by comprising:

a fluorescence collection device, a scan acquisition controller, a femtosecond pulse laser, an optical fiber coupling module, and the zoom type cavity endoscope detection device of any one of claims 1-7, wherein the fluorescence collection device and the optical fiber coupling module are both in optical fiber communication connection with the zoom type cavity endoscope detection device, and are both electrically connected with the scan acquisition controller, wherein:

the femtosecond pulse laser is used for outputting pulse laser signals to the optical fiber coupling module;

the optical fiber coupling module is used for coupling the pulse laser signal output by the femtosecond pulse laser and transmitting the pulse laser signal to the collimating lens in the zoom type cavity endoscope detection device;

the zoom type cavity endoscope detection device is used for receiving the pulse laser signal, outputting the pulse laser signal to an autofluorescent substance in a cell of a living body, acquiring a fluorescence signal and a second harmonic signal generated after the autofluorescent substance is excited through the objective lens, and outputting the fluorescence signal and the second harmonic signal to the fluorescence collection device;

the fluorescence collecting device is used for receiving the fluorescence signal and the second harmonic signal and then respectively converting the fluorescence signal and the second harmonic signal into corresponding electric signals;

and the scanning acquisition controller is used for controlling the micro-electromechanical scanning galvanometer to scan the pulse laser signals and synchronously acquire the electric signals.

9. The three-dimensional nonlinear laser scanning cavity endoscope of claim 8, further comprising an illumination module and an imaging module, both of which are in fiber-optic communication connection with the cavity endoscope detection device, wherein:

the illumination module sequentially comprises an illumination lens, a variable optical filter and an illumination light source, the illumination lens is connected with the illumination optical fiber bundle, and the illumination light source is used for providing an illumination light signal;

the imaging module sequentially comprises an imaging lens and a camera, the imaging lens is connected with the bright field optical fiber bundle, and the camera is used for acquiring image information of a tissue area to be detected.

10. The three-dimensional nonlinear laser scanning cavity endoscope in accordance with claim 8, wherein the fluorescence collecting device comprises a collecting fiber optic universal interface, a first photomultiplier tube, a second photomultiplier tube, and a first collecting light path between the collecting fiber optic universal interface and the first photomultiplier tube, a second collecting light path between the collecting fiber optic universal interface and the second photomultiplier tube, wherein:

the first collecting light path sequentially comprises a coupling lens, an infrared filter, a first dichroic mirror, a first filter and a first collecting lens, wherein the first collecting light path is used for collecting the fluorescent signals received by the fluorescent collecting device, and the first photomultiplier is used for converting the fluorescent signals into first electric signals;

the second collecting light path sequentially comprises the coupling lens, the infrared filter, the first dichroic mirror, the second filter and the second collecting lens, wherein the second collecting light path is used for collecting the second harmonic signals received by the fluorescence collecting device, and the second photomultiplier is used for converting the second harmonic signals into second electric signals.