CN210055941U - Cavity endoscope detection device with adjustable objective lens and laser scanning cavity endoscope - Google Patents
Cavity endoscope detection device with adjustable objective lens and laser scanning cavity endoscope Download PDFInfo
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- CN210055941U CN210055941U CN201920180164.5U CN201920180164U CN210055941U CN 210055941 U CN210055941 U CN 210055941U CN 201920180164 U CN201920180164 U CN 201920180164U CN 210055941 U CN210055941 U CN 210055941U
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Abstract
The embodiment of the utility model provides a cavity endoscope detection device and laser scanning cavity endoscope with adjustable objective. The cavity endoscope detection device comprises a handle shell and a detection tube, wherein a relay lens and an infinite objective lens are arranged in a detection channel of the detection tube, a first light path and a second light path are formed, and the first light path comprises a collimating lens, a micro-electro-mechanical scanning galvanometer, a lens, a dichroic mirror, a relay lens and an infinite objective lens; the second optical path includes an infinity objective, a relay, and a dichroic mirror. The embodiment of the utility model provides a cavity endoscope detection device and three-dimensional nonlinear laser scanning cavity endoscope zoom the motor through the high accuracy in the detection passageway, drive infinity objective reciprocates, through zooming the operation to the histocyte that realizes the different degree of depth is surveyed, acquires the cell structure information of the different degree of depth, in order to satisfy the detection needs to gastrointestinal tract tissue and oral cavity tissue etc. easy operation, convenient to use.
Description
Technical Field
The embodiment of the utility model provides a relate to laser scanning endoscope technical field, especially relate to a cavity endoscope detection device and laser scanning cavity endoscope with adjustable objective.
Background
Gastrointestinal malignancies are the second leading cause of cancer death in the population of developed countries and this trend has become more evident in recent years. The treatment of gastrointestinal malignant tumor mainly adopts surgical radical resection, but the specific range of the surgical resection needs to be determined when the surgical radical resection is specifically carried out, so before the operation, the benign and malignant tumor, the infiltration depth, the metastasis condition, the existence of cancer residue at the cut edge and the like need to be known. Preoperative gastrointestinal endoscopic biopsy is therefore a very important diagnostic evidence for histological diagnosis of gastrointestinal tumors. The operative form of stomach cancer is divided into full stomach resection, secondary stomach resection, partial gastrectomy, endoscopic mucosal or submucosal resection and the like according to the size, the growth position, the infiltration depth and the like of a tumor body.
Currently, gastrointestinal endoscopic biopsy is usually based on gastrointestinal endoscopy, imaging is performed with the aid of CT, MRI, etc., or a number of gastrointestinal diseases are evaluated with a conventional white light laparoscope or endoscope.
However, there are some inevitable disadvantages in imaging by imaging using CT, MRI, etc. based on enteroscopy, such as easy bleeding of intestinal tube or tumor body in operation, manual pulling or squeezing, delay in time due to repeated endoscopic biopsy when enteroscopy cannot pass through the intestinal tube, and additional emergency hemostasis if severe bleeding is caused. In clinical practice, auxiliary examination means such as CT and MRI cannot accurately judge the infiltration depth of early gastrointestinal tumors and lymph node metastasis. The gastrointestinal tumor T stage is judged by ultrasonic endoscopy, and the accuracy reported in the literature is only 44.7% -78%, which is not enough to become a reliable diagnostic standard. The ultrasonic endoscope also has poor preoperative evaluation effect on the local resection, cannot accurately subdivide gastrointestinal mucosa levels, and has poor N staging effect. Therefore, in view of the current gastrointestinal auxiliary diagnostic technology, a new gastrointestinal tumor diagnostic device is urgently needed to detect the gastrointestinal tissue information in situ and in real time.
SUMMERY OF THE UTILITY MODEL
To the technical problem who exists among the prior art, the embodiment of the utility model provides a cavity endoscope detection device and laser scanning cavity endoscope with adjustable objective.
In a first aspect, an embodiment of the present invention provides a cavity endoscope detection device with adjustable objective lens, including:
handle casing and detecting tube, handle casing with detecting tube fixed connection, be provided with the detection passageway in the detecting tube, be provided with relay lens, infinite objective and be used for the drive in the detection passageway the zoom motor that infinite objective reciprocated, infinite objective with the zoom motor all is located the access point department of detection passageway, infinite objective the relay lens with the light path structure that sets up in the handle casing forms first light path and second light path, wherein:
the first optical path sequentially comprises a collimating lens, a micro-electromechanical scanning galvanometer, a first lens, a second lens, a dichroic mirror, the relay lens and the infinity objective lens, wherein the collimating lens, the micro-electromechanical scanning galvanometer, the first lens, the second lens, the dichroic mirror, the relay lens and the infinity objective lens are positioned between a general optical fiber interface and the passage port in the handle shell, and the first optical path is used for conducting a laser signal received by the collimating lens from the general optical fiber interface to the passage port;
the second optical path sequentially includes the infinity objective lens, the relay lens and the dichroic mirror between the channel port and the optical fiber universal interface, where the second optical path is used to conduct the optical signal collected by the infinity objective lens from the channel port to the optical fiber universal interface.
In a second aspect, an embodiment of the present invention provides an endoscope in a three-dimensional nonlinear laser scanning cavity, including:
fluorescence collection device, scanning acquisition controller, femto second pulse laser instrument, fiber coupling module and the embodiment of the utility model provides a cavity speculum detecting device with adjustable objective that the first aspect provided, fluorescence collection device with fiber coupling module all with cavity speculum detecting device fiber communication connection with adjustable objective, fluorescence collection device with cavity endoscope detecting device with adjustable objective all with scanning acquisition controller electricity is connected, wherein:
the femtosecond pulse laser is used for outputting pulse laser signals to the optical fiber coupling module;
the optical fiber coupling module is used for coupling the pulse laser signal output by the femtosecond pulse laser and transmitting the pulse laser signal to the collimating lens in the cavity endoscope detection device with the adjustable objective lens;
the cavity endoscope detection device with the adjustable objective lens is used for receiving the pulse laser signal, outputting the pulse laser signal to an autofluorescence substance in a cell of a living body, acquiring a fluorescence signal and a second harmonic signal generated after the autofluorescence substance is excited by the infinity objective lens, and outputting the fluorescence signal and the second harmonic signal to the fluorescence collection device;
the fluorescence collecting device is used for receiving the fluorescence signal and the second harmonic signal and then respectively converting the fluorescence signal and the second harmonic signal into corresponding electric signals;
and the scanning acquisition controller is used for controlling the micro-electromechanical scanning galvanometer to scan the pulse laser signals and synchronously acquire the electric signals.
The embodiment of the utility model provides a cavity endoscope detection device with adjustable objective and laser scanning cavity inside speculum adopt the constitution mode of handle casing and detecting tube fixed connection, set up first light path and second light path in the inner space of two component parts, form the cavity inside speculum detection device with adjustable handheld objective, first light path includes collimating lens, liquid lens, micro-electromechanical scanning galvanometer, first lens, second lens, dichroic mirror, relay mirror and infinity objective for the laser signal of conduction excitation autofluorescence material; the second light path includes infinity objective and dichroic mirror for collect two-photon signal and second harmonic signal, wherein, zooms the motor through the high accuracy in the detection passageway, drive infinity objective reciprocates, through zooming the operation, surveys in order to realize the histiocyte of the different degree of depth, acquires the cell structure information of the different degree of depth, in order to satisfy the detection needs to human abdominal cavity internal gastrointestinal tract tissue, oral cavity tissue and palace intracavity tissue, easy operation, convenient to use.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic structural view of a cavity endoscope detection device with an adjustable objective lens according to an embodiment of the present invention;
FIG. 2 is a schematic structural view of an endoscopic cavity detection device with an adjustable objective lens according to another embodiment of the present invention;
fig. 3 is a schematic structural view of a cavity endoscope detection device with an adjustable objective lens according to another embodiment of the present invention;
FIG. 4 is a schematic structural view of an endoscopic cavity detection device with an adjustable objective lens according to another embodiment of the present invention;
fig. 5 is a schematic view of an endoscope structure in a three-dimensional nonlinear laser scanning cavity according to an embodiment of the present invention;
fig. 6 is a schematic view of an endoscope structure in a three-dimensional nonlinear laser scanning cavity according to another embodiment of the present invention;
fig. 7 is a schematic structural view of a fluorescence collecting device according to an embodiment of the present invention;
fig. 8 is a schematic view of a box sealing structure of a box combination structure of a three-dimensional nonlinear laser scanning cavity endoscope according to an embodiment of the present invention;
fig. 9 is a schematic view of a box sealing structure of a box combination structure of a three-dimensional nonlinear laser scanning cavity endoscope according to another embodiment of the present invention;
fig. 10 is a schematic view of a table-type structure of an endoscope in a three-dimensional nonlinear laser scanning cavity according to an embodiment of the present invention;
fig. 11 is a schematic diagram of a table-top configuration of a three-dimensional nonlinear laser scanning cavity endoscope according to another embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
At present, the gastrointestinal endoscope is taken as a basis, imaging is carried out by taking CT, MRI and the like as assistance to obtain relevant information of malignancy, infiltration depth, metastasis condition, whether cancer residue exists at incisal margin and the like of tumors, and the method has some defects in specific operation, such as easy bleeding of intestinal tracts or tumor bodies, need of manual drawing or extrusion, delay of time due to repeated endoscopic biopsy when the gastrointestinal endoscope cannot pass through the intestinal tracts, and need of additional emergency hemostasis if severe bleeding is caused. In clinical practice, auxiliary examination means such as CT and MRI cannot accurately judge the infiltration depth of early gastrointestinal tumors and lymph node metastasis. The gastrointestinal tumor T stage is judged by the ultrasonic endoscope, the accuracy reported in the literature is only 44.7% -78%, the judgment is not enough to become a reliable diagnosis standard, the preoperative judgment effect of the ultrasonic endoscope on the local resection operation is poor, the gastrointestinal mucosa levels cannot be accurately subdivided, and the N stage effect is also poor.
While conventional white light laparoscopes and endoscopes can assess many gastrointestinal diseases, the technique is limited to detecting gross morphological changes. Although suspicious regions are easily found, these techniques are associated with false positive rates, specificity, and the like, as compared to in vivo detection techniques. White light endoscopy is associated with wide error in diagnosis of microscopic changes, including examination diagnosis of ulcerative colitis or Barrett's esophagus and flat adenomatous dysplasia. Confocal endoscopy combines laser technology, fluorescence detection technology, rapid scanning technology and the like, and is concerned widely because the confocal endoscope can detect mucosal changes at a microscopic level and can possibly be used for replacing tissue biopsy, and the imaging technology has high sensitivity and specificity. However, the confocal endoscopic imaging technology is still limited by the imaging depth and the fluorescent staining agent, because the gastrointestinal sample has strong absorption and scattering to visible light, the imaging depth is only on the superficial layer, and a specific fluorescent staining developer is required to be injected, the operation is too complicated, and the relevant information such as the infiltration depth of the tumor, the metastasis condition, the existence of cancer residue at the surgical incisal margin and the like cannot be accurately obtained.
The two-photon microscopic imaging technology adopts a femtosecond pulse laser with longer wavelength as an excitation light source, has the characteristics of deep imaging depth, small light damage, small photobleaching area, high fluorescence collection efficiency and the like, and has epoch-making significance in deep imaging of biological tissues. The first two-photon fluorescence microscope in the world was developed by w.denk et al at cornell university in 1990, using a multi-photon microscopic imaging technique based on nonlinear optics and femtosecond pulsed laser. The technology can rapidly obtain the tissue structure and the cell morphology of the specimen in real time by utilizing autofluorescence generated by cells in living tissues and second harmonic generated by collagen tissues. As early as 1986, the second harmonic was used for skin studies and coronary microscopic imaging studies, confirming its feasibility for being used to observe biological tissues. MPM is also an important tool for cancer research. The autofluorescence generated by the cell is derived from Nicotinamide Adenine Dinucleotide (NADH) and Flavin Adenine Dinucleotide (FAD) in the cell, the wavelength emitted by NADH is 460nm, and the second oscillation harmonic of collagen is 370-390 nm, so that a multiphoton microscope with a range of 780-940 nm is usually selected when observing tumor sample tissues. Not only does MPM imaging match standard tumor histopathology, it also provides additional information about the tumor neogenesis process, as can the metabolic levels of tumor tissue cells by measuring the ratio NADH/FAD.
With multiphoton imaging techniques, multiphoton microscopy is capable of providing real-time gastrointestinal tract tissue structure and cell morphology information. The multi-photon imaging technology has the characteristics of no need of exogenous labeled tissues, extremely sensitivity to collagen, small photodamage to tissues, deep penetration depth and the like, and can be possibly applied to optical biopsy of gastrointestinal tumors. There are no clinically available two-photon laparoscopes and endoscopes, and no objective-adjustable intracavity endoscope detection device based on two-photon imaging, to detect gastrointestinal tract tissue information in situ and in real time.
For the different degree of depth's of normal position real-time detection intestines and stomach tissue information, the embodiment of the utility model provides a cavity endoscope detection device with adjustable objective, figure 1 does the utility model relates to an embodiment provides a cavity endoscope detection device with adjustable objective structural schematic diagram, as shown in figure 1, this cavity endoscope detection device with adjustable objective includes:
handle casing 11 and detecting tube 12, handle casing 11 with detecting tube 12 fixed connection, be provided with the detection passageway in the detecting tube, be provided with relay lens 117, infinity objective 118 and be used for the drive in the detection passageway infinity objective reciprocates zoom motor 119, infinity objective 118 with zoom motor 119 all is located detect the passageway department of passageway, infinity objective 118 relay lens 117 with the light path structure that sets up in the handle casing forms first light path and second light path, wherein:
the first optical path sequentially comprises a collimating lens 112, a micro-electromechanical scanning galvanometer 113, a first lens 114, a second lens 115, a dichroic mirror 116, the relay mirror 117 and the infinity objective 118, which are located between a general optical fiber interface 111 and the passage port in the handle housing, wherein the first optical path is used for conducting the laser signal received by the collimating lens 112 from the general optical fiber interface to the passage port;
the second optical path sequentially includes the infinity objective 118, the relay 117 and the dichroic mirror 116 between the channel port and the fiber-optic common interface, wherein the second optical path is used for conducting the optical signal collected by the infinity objective 118 from the channel port to the fiber-optic common interface.
Specifically, the cavity endoscope detection device with the adjustable objective lens provided by the embodiment of the invention integrates two optical paths, one is a first optical path for conducting a laser signal, and the laser signal is mainly used for exciting a spontaneous fluorescent substance; the other is to collect and transmit two-photon signals and second harmonic signals generated after the spontaneous fluorescent substance is excited; wherein, the partial optical path structure in these two optical paths includes collimating lens 112, micro-electromechanical scanning galvanometer 113, first lens 114, second lens 115 and dichroic mirror 116, and is integrated in handle shell 11, and relay lens 117 and infinity objective 118 are integrated in the detection tube, and infinity objective 118 is set at the passage mouth of the detection passage, and passage mouth still is provided with zoom motor 119, such as high precision zoom motor of voice coil motor, and is used for driving infinity objective to move up and down, through zooming operation, to realize tissue cell detection of different depths, obtain cell structure information of different depths, wherein infinity objective can be installed in high precision zoom motor.
The relay lens 117 is arranged in the detection channel at the inner side of the infinity objective lens, the relay lens 117 is used for conducting two-photon signals and second harmonic signals collected by the infinity objective lens to the dichroic mirror in a long distance, an image plane of the infinity objective lens 118 is overlapped with a focal plane of the relay lens 117, the relay lens is used for image transmission firstly, and a laser signal scanning area passing through the micro-electromechanical scanning mirror is conducted to an image plane of the infinity objective lens 118 in an equal ratio of 1: 1. Secondly, the relay lens also conducts the optical signal collected by the infinity objective lens to the optical path structure part in the handle shell and collects the optical signal through the optical fiber bundle of the optical path structure, wherein the dichroic mirror 116 can be a long-pass short-dichroic mirror or a short-pass long-dichroic mirror, namely when the long-pass short-dichroic mirror is arranged, a pulse laser signal for exciting the autofluorescence is transmitted, and the collected two-photon signal and the second harmonic signal are reflected, as shown in fig. 1, at this time, the objective lens adjustable cavity endoscope detection device can be a detection device of the laparoscope; fig. 2 is a schematic structural diagram of a cavity endoscope detection device with an adjustable objective lens according to another embodiment of the present invention, as shown in fig. 2, when the dichroic mirror 116 is a short-pass long-reflective dichroic mirror, a pulse laser signal for exciting an autofluorescence substance is reflected, a two-photon signal and a second harmonic signal are collected by transmission, the dichroic mirror reflects a two-photon signal and a second harmonic signal emitted from the optical fiber general interface 111 and incident on the dichroic mirror 116 through the collimating lens 112, the micro-electromechanical scanning galvanometer 113, the first lens 114, and the second lens 115 to the infinity objective 118, and the infinity objective 118 collects the two-photon signal and the second harmonic signal, at this time, the cavity endoscope detection device with the adjustable objective lens can be a detection device of a mouth mirror, wherein the detection device of the mouth mirror also includes two parts, namely, a handle housing 11 and a detection tube 12.
The embodiment of the utility model provides a cavity endoscope detection device with adjustable objective adopts the constitution mode of handle casing and detecting tube fixed connection, sets up first light path and second light path in the inner space of two component parts, forms the cavity endoscope detection device with adjustable handheld objective, and first light path includes collimating lens, liquid lens, micro-electromechanical scanning galvanometer, first lens, second lens, dichroic mirror, relay lens and infinite objective for the laser signal of conduction excitation autofluorescence material; the second light path includes infinity objective and dichroic mirror for collect two-photon signal and second harmonic signal, wherein, zooms the motor through the high accuracy in the detection passageway, drive infinity objective reciprocates, through zooming the operation, surveys in order to realize the histiocyte of the different degree of depth, acquires the cell structure information of the different degree of depth, in order to satisfy the detection needs to human abdominal cavity internal gastrointestinal tract tissue, oral cavity tissue and palace intracavity tissue, easy operation, convenient to use.
On the basis of each embodiment, the embodiment of the utility model provides an optical path structure among adjustable cavity endoscope detection device of objective still includes liquid lens, fig. 3 is the utility model discloses adjustable cavity endoscope detection device of objective structure schematic diagram that still another embodiment provided, as shown in fig. 3, liquid lens 110 is located collimating lens 112 and the micro-electromechanical scanning mirror 113 between to form new first light path, new first light path includes collimating lens 112, liquid lens 110, the micro-electromechanical scanning mirror 113 that shakes, first lens 114, second lens 115, dichroic mirror 116 and infinity objective 118 that are located between optical fiber general interface 111 and the entrance. That is, the liquid lens is arranged such that the surface of the liquid lens 110 can be correspondingly curved by applying a voltage or a current to the liquid lens, and thus the parallel light emitted from the collimating lens 112 can be aligned to generate different powers. The specific light path is as follows: laser signals are emitted from the optical fibers, parallelly enter the liquid lens after passing through the collimating lens, corresponding focal power is generated from the liquid lens 110 according to loaded voltage or current signals, and emitted convergent or divergent light passes through the micro-electro-mechanical scanning galvanometer, the first lens, the second lens and the dichroic mirror, is transmitted to the infinity objective through the relay lens, and is converged on a sample. The focal power change introduced by the liquid lens can enable the focal point of a laser signal emitted from an infinite objective lens port to move back and forth in the depth direction, the response speed of the liquid lens is very high, and the scanning frequency of the liquid lens is in the order of KHz, so that rapid depth direction scanning imaging can be realized. The liquid lens is equivalent to parallel plate glass when no voltage or current signal is applied, and has no focal power to the laser signal and can not cause any deviation of a focus behind an infinite objective lens, so that three-dimensional imaging is realized. When the cavity endoscope detection device is used specifically, the liquid lens is complementary with the zoom motor 119, the position of the infinite objective lens is adjusted through the zoom motor 119, after the system is roughly adjusted to the corresponding depth position, the system is switched to a liquid lens zoom scanning mode, and a sample is rapidly three-dimensionally imaged, wherein when the cavity endoscope detection device is not provided with the zoom motor 119, the zoom adjustment can be performed only through the liquid lens.
On the basis of each embodiment, the embodiment of the utility model provides a still be provided with a plurality of illumination passageways in the detection pipe among the adjustable cavity endoscope detection device of objective, fig. 4 is the utility model discloses cavity endoscope detection device with adjustable objective structure sketch map that still another embodiment provided is shown in fig. 4, is provided with the illumination fiber bundle that is used for transmitting illumination optical signal in the illumination passageway 123, and wherein illumination passageway 123 uses the axle center of surveying the passageway as center evenly distributed. Namely the embodiment of the utility model provides a still be provided with a plurality of illumination passageways 123 in the detection pipe among the cavity endoscope detection device with adjustable objective, this illumination passageway 123 is more than one, all be provided with the illumination fiber bundle in every passageway, illumination fiber has certain aperture angle, do not need lens can directly be used for dispersing the illumination, and illumination passageway 123 uses the axle center of detecting the passageway as central evenly distributed, provide even illumination for cavity endoscope detection device with adjustable objective to the tissue area state that awaits measuring before convenient work observation infinity objective.
On the basis of each embodiment, the embodiment of the utility model provides a still be provided with in the detecting tube among the adjustable cavity endoscope detecting device of objective and observe the passageway, as shown in fig. 4, observe the passageway and be located between detecting channel and the illumination passageway, wherein:
an observation lens 124 is arranged at the channel opening of the observation channel, and the observation lens 124 is connected with the bright field optical fiber bundle in the observation channel to acquire image information of the tissue area to be detected in front of the infinity objective. That is to say the embodiment of the utility model provides a still be provided with in the probe tube among the adjustable cavity endoscope detecting device of objective and survey the passageway, should survey the passageway and be located between survey passageway and the illumination channel, and be provided with observation lens 124 and bright field fiber bundle, bright field fiber bundle is imaging fiber bundle promptly for the tissue region image information that awaits measuring before the infinity objective that transmission observation lens 124 caught, wherein survey the passageway and can be one, also can be for two formation two mesh observations, realize the internal speculum function of three-dimensional bright field cavity.
On the basis of the above embodiments, the embodiment of the present invention provides a suction channel is further disposed in the detection tube of the cavity endoscope detection device with an adjustable objective lens, as shown in fig. 4, the suction channel 125 is located between the illumination channel and the edge of the detection tube. That is to say, the embodiment of the utility model provides a still be provided with in the probe tube among the adjustable cavity endoscope detecting device of objective and be used for making the adjustable cavity endoscope detecting device of objective adsorb the absorption passageway 125 on the tissue that awaits measuring, through the air in the extraction absorption passageway 125, form the negative pressure in absorption passageway 125 for the adjustable cavity endoscope detecting device of objective adsorbs on the tissue that awaits measuring, wherein, it is located between illumination passageway and the probe tube edge to adsorb passageway 125, is located the illumination passageway outside promptly, is close to the position department of probe tube avris.
On the basis of each embodiment, the embodiment of the utility model provides a button hole has been seted up on the handle casing among the adjustable cavity endoscope detecting device of objective, and the button hole is provided with the switching button, and the switching button is used for switching different light filters to acquire the illumination light signal of different wavelengths. Namely, the embodiment of the utility model provides a switching button is provided with in the button hole of the handle casing among the adjustable cavity endoscope detection device of objective, can switch the light filter of filtering different wavelength illumination light signal through this switching button for the staff can select the illumination light signal of the different wavelength of coming of transmission, and wherein the function of switching button is still accessible software self-defined, modifies its corresponding function.
On the basis of each embodiment, the embodiment of the utility model provides a button hole still is provided with the imaging button among the adjustable cavity endoscope detecting device of objective, and the imaging button is used for controlling the imaging module who is connected with the bright field fiber bundle and images to the tissue region that awaits measuring before the infinity objective. Namely the embodiment of the utility model provides a cavity endoscope detection device with adjustable objective in handle casing's button hole is provided with the imaging button, can control the imaging module that is connected with bright field fiber bundle through this imaging button and shoot the formation of image to the tissue region that awaits measuring before the infinity objective, wherein the function of imaging button still accessible software is self-defined, modifies its corresponding function.
On the basis of the above embodiments, the embodiment of the present invention provides a suction channel in a cavity endoscope detection device with an adjustable objective lens, which is an annular channel or a plurality of circular channels. The adsorption channel used for enabling the cavity endoscope detection device with the adjustable objective lens to be adsorbed on the tissue to be detected can be an annular channel along the inner side of the detection tube or a plurality of circular channels so as to form enough negative pressure, and the cavity endoscope detection device with the adjustable objective lens is adsorbed on the tissue to be detected.
The embodiment of the utility model provides a still provide a three-dimensional nonlinear laser scanning cavity inside speculum, fig. 5 does the utility model relates to a three-dimensional nonlinear laser scanning cavity inside speculum structure sketch map that the embodiment provided, as shown in fig. 5, this three-dimensional nonlinear laser scanning cavity inside speculum includes:
the femtosecond pulse laser is used for outputting pulse laser signals to the optical fiber coupling module;
the optical fiber coupling module is used for coupling the pulse laser signal output by the femtosecond pulse laser and transmitting the pulse laser signal to a collimating lens in the cavity endoscope detection device 1 with the adjustable objective lens;
the cavity endoscope detection device 1 with the adjustable objective lens is used for receiving the pulse laser signal, outputting the pulse laser signal to the autofluorescence substance in the living body cell, acquiring a fluorescence signal and a second harmonic signal generated after the autofluorescence substance is excited through the infinity objective lens, and outputting the fluorescence signal and the second harmonic signal to the fluorescence collection device 56;
a fluorescence collecting device 56 for receiving the fluorescence signal and the second harmonic signal and converting the fluorescence signal and the second harmonic signal into corresponding electrical signals;
and the scanning acquisition controller 531 is used for controlling the micro-electromechanical scanning galvanometer to scan the pulse laser signals and synchronously acquire electric signals.
Specifically, the embodiment of the present invention provides a three-dimensional nonlinear laser scanning cavity endoscope, which comprises a fluorescence collecting device 56, a scanning collecting controller 531, a femtosecond pulse laser, a fiber coupling module and a cavity endoscope detecting device 1 with an adjustable objective lens, thereby forming a three-dimensional nonlinear laser scanning cavity endoscope for detecting gastrointestinal tissues and oral tissues by using a two-photon imaging technology, wherein the femtosecond pulse laser can emit pulse laser signals for exciting autofluorescence in gastrointestinal tissues and oral tissue cells of a human body, generating multiphoton fluorescence signals and second harmonic signals, including using the 920nm femtosecond pulse laser to excite FAD and collagen in cells, exciting 500-600nm fluorescence signals and 460nm second harmonic signals, and exciting autofluorescence such as FAD or NADH in cells by 780nm femtosecond pulse laser, to generate corresponding fluorescence signal and second harmonic signal, wherein the femtosecond pulse laser and the fiber coupling module are combined together to form a laser emission module 540;
the fluorescence collecting device 56 integrates two signal collecting optical paths, namely a fluorescence signal collecting optical path and a second harmonic signal collecting optical path, to respectively collect the fluorescence signal and the second harmonic signal; the scanning acquisition controller 531 controls the micro-electromechanical scanning galvanometer to scan the pulse laser signals and excite the autofluorescence substance to generate fluorescence signals and second harmonic signals, and acquires first electric signals and second electric signals obtained by converting the fluorescence signals and the second harmonic signals by the fluorescence collecting device 56; the three-dimensional nonlinear laser scanning cavity endoscope can be divided into a laparoscope and a mouth mirror according to the difference of the structures of the cavity endoscope detection devices 1 with adjustable objective lenses. The resolution of the three-dimensional nonlinear laser scanning cavity endoscope can be set to 800nm, the imaging field of view can be 400 micrometers by 400 micrometers, and the imaging speed can be 26 frames (256 pixels by 256) or 13 frames (512 pixels by 512 pixels).
The embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope adopts fluorescence collection device, the scanning acquisition controller, femto second pulse laser, fiber coupling module and the adjustable cavity endoscope detection device of objective, thereby form the three-dimensional nonlinear laser scanning cavity endoscope that utilizes two-photon imaging technique to survey human intestines and stomach tissue and oral cavity tissue, adjust infinite objective focal length through liquid lens, realize laser scanning microscope's three-dimensional scanning, excite intracellular autofluorescence material and obtain multiphoton fluorescence signal and second harmonic signal through femto second pulse laser, realize laser scanning microscope nonlinearity, collect fluorescence signal and second harmonic signal through fluorescence collection device, and convert corresponding electric signal into, and then obtain corresponding fluorescence image etc. that reflects cell tissue structure through this electric signal, wherein, the adoption of the cavity endoscope detection device with the adjustable objective lens can make the staff can be nimble to survey the gastrointestinal tissue, the oral tissue and the intrauterine tissue in the abdominal cavity of the human body, when surveying the gastrointestinal tissue of the human body, only need to set up a small opening to the abdomen of the human body, so the operation cost and the pain of a patient can be reduced, when imaging the intrauterine tissue, the noninvasive detection can be carried out through a natural channel (vagina), the equipment operation is simple, and the use is convenient.
On the basis of each embodiment, the embodiment of the utility model provides a cavity endoscope is scanned to three-dimensional nonlinear laser still includes lighting module and imaging module, as shown in fig. 5, lighting module 534 and imaging module 533 are all with the cavity endoscope detection device optical fiber communication connection that objective is adjustable, wherein:
the illumination module 534 sequentially comprises an illumination lens 5342, a variable filter 5341 and an illumination light source 5343, wherein the illumination lens 5342 is connected with the illumination optical fiber bundle, and the illumination light source is used for providing an illumination light signal;
the imaging module 533 sequentially includes an imaging lens 5331 and a camera 5332, the imaging lens 5331 is connected to the bright field optical fiber bundle, and the camera 5332 is configured to acquire image information of a tissue region to be measured. That is to say, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope still is provided with illumination module 534 and imaging module 533, wherein, illumination module 534 includes illuminating lens 5342, variable optical filter 5341 and illuminating light source 5343 in proper order, wherein, illuminating light source can switch different optical filters through electronic variable optical filter runner, in order to obtain the illuminating light signal of different wavelengths, the fundamental principle is that two-photon fluorescence imaging is not disturbed, for example when obtaining autofluorescence and second harmonic, can switch to red or infrared optical filter 635, in order to obtain 370nm, illuminating light nm or infrared 850nm, the illuminating light signal of 940nm, illuminating light signal gets into illumination fiber bundle through the lens coupling;
the imaging module 533 sequentially includes an imaging lens and a camera, the lens being focused on the camera for direct observation of bright field. The two cameras correspond to binocular bright field optical fiber bundles, bright field imaging and two-photon imaging form a multi-mode laparoscope, and bright field binocular three-dimensional laparoscope is in a mode, so that large-view sample observation is carried out, and the basic appearance of a sample is mainly observed. For the suspicious or interested area, the method can be switched to a two-photon mode to perform autofluorescence and second harmonic imaging, and observe the cell grade morphology of the sample, thereby providing a basis for further judgment. Wherein the camera may be an imaging device based on an imaging device such as a CCD or CMOS.
On the basis of each embodiment, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope, still include air exhaust device, as shown in fig. 5, air exhaust device 52 mainly includes the aspiration pump, link to each other with the absorption passageway through the exhaust pipe, set up the bleeder valve in the exhaust pipe, the bleeder valve is connected with air exhaust device 52 electricity, air exhaust device 52 is through the switch of adjustment bleeder valve and the size of switching, the bleed-off flow of control bleeder pipe, thereby realize the control of bleeding to the absorption passageway, and then adjust the negative pressure in the absorption passageway, make the adjustable cavity endoscope detection device of objective pass through the effect of atmospheric pressure, adsorb on tissues such as human intestines and stomach or oral cavity, reduce the motion artifact that biological tissue activity brought, make the formation of image more stable, it is clear.
On the basis of above-mentioned each embodiment, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope still includes the industrial computer, as shown in fig. 5, industrial computer 532 is connected with scanning acquisition controller 531 electricity, wherein:
the industrial personal computer 532 is configured to obtain the first electrical signal and the second electrical signal acquired by the scanning acquisition controller 531, generate a first fluorescent image based on the first electrical signal, and generate a second fluorescent image based on the second electrical signal. Namely the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope still includes the industrial computer 532 with scan acquisition controller 531 electricity is connected, this industrial computer 532 is based on first signal of telecommunication generation first fluorescence image and based on the second signal of telecommunication generation second fluorescence image, can be used for showing cell structure and fibrous structure information respectively, wherein installs control software on the industrial computer, through control software, sends control command to the scanner to control scan acquisition controller, acquire above-mentioned first signal of telecommunication and second signal of telecommunication.
On the basis of above-mentioned each embodiment, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope still includes the display, as shown in fig. 5, display 55 is connected with industrial computer 532 electricity for show first fluorescence image and second fluorescence image. Namely, the embodiment of the utility model provides a three-dimensional nonlinear laser scanning cavity endoscope is still including being used for showing display 55 of first fluorescence image and second fluorescence image, through display 55, the staff can directly acquire the relevant information of first fluorescence image and second fluorescence image.
Wherein, fig. 6 is a schematic view of the endoscope structure in the three-dimensional nonlinear laser scanning cavity provided by another embodiment of the present invention, as shown in fig. 6, the endoscope structure in the three-dimensional nonlinear laser scanning cavity also includes:
the fluorescence collection device 56, the scanning collection controller 531, the femtosecond pulse laser, the fiber coupling module, and the cavity endoscope detection device 1 with adjustable objective lens, the air extractor 52, the industrial personal computer 532, the illumination module 534 and the imaging module 533 provided by the above embodiments, wherein the fluorescence collection device 56 and the fiber coupling module are both in fiber communication connection with the cavity endoscope detection device 1 with adjustable objective lens, the fluorescence collection device 56 and the cavity endoscope detection device 1 with adjustable objective lens are both electrically connected with the scanning collection controller 531, wherein the functions of the above modules or devices are the same as the functions of the devices in the above embodiments, the modules or devices comprise the femtosecond pulse laser and the fiber coupling module which are combined together to form the laser emission module 540, the illumination module 534 sequentially comprises an illumination lens 5342, a variable optical filter 5341 and an illumination light source 5343, and the imaging module 533 sequentially comprises an imaging lens and a camera, the cavity endoscope detection device 1 with the adjustable objective lens in the three-dimensional nonlinear laser scanning cavity endoscope is a mouth endoscope detection device, a light path structure of the cavity endoscope detection device with the adjustable objective lens comprises a liquid lens, the function of the liquid lens is the same as that of the liquid lens in each embodiment, and the light path is the same as that of the corresponding light path in each embodiment.
On the basis of the foregoing embodiments, fig. 7 is a structural schematic diagram of the fluorescence collecting device provided by the embodiment of the present invention, as shown in fig. 7, the embodiment of the present invention provides a fluorescence collecting device including collecting the general optical fiber interface 881, the first photomultiplier 882, the second photomultiplier 883, and the first collecting optical path between the collecting general optical fiber interface 881 and the first photomultiplier 882, and the second collecting optical path between the collecting general optical fiber interface 881 and the second photomultiplier 883, wherein:
the first collecting light path sequentially comprises a coupling collecting lens 81, an infrared filter 82, a first dichroic mirror 83, a first filter 84 and a first collecting lens 85, wherein the first collecting light path is used for collecting the fluorescent signal received by the fluorescent collecting device, and the first photomultiplier 882 is used for converting the fluorescent signal into a first electrical signal;
the second collecting light path sequentially includes a coupling collecting lens 81, an infrared filter 82, a first dichroic mirror 83, a second dichroic mirror 86, a second filter 87, and a second collecting lens 88, wherein the second collecting light path is configured to collect a second harmonic signal received by the fluorescence collecting device, and the second photomultiplier 883 is configured to convert the second harmonic signal into a second electrical signal. That is, the fluorescence collecting device provided by the embodiment of the present invention has a two-way signal collecting function, and integrates two light paths, wherein the first dichroic mirror 83 in the first collecting light path is a transmission fluorescence signal, the dichroic mirror for reflecting the second harmonic, the second dichroic mirror 86 and the first dichroic mirror 83 are the same dichroic mirror, and are used for reflecting the second harmonic, the first optical filter 84 is used for transmitting the fluorescence signal, and filtering the rest interference signals, and the second optical filter 87 is used for transmitting the corresponding second harmonic signal, and filtering the rest interference signals, for example, when using 780nm femtosecond fiber laser to excite the autofluorescence substance in the human abdominal cavity or oral cavity cell, 390nm second harmonic signal and 450 and 600nm two-photon autofluorescence signal can be obtained, the wavelength passes through above 420nm, the dichroic mirror with wavelength reflection below 420, that is, the first dichroic mirror 83, can separate two paths of fluorescence, clean second harmonic signals and fluorescence signals can be obtained by using the first filter 84 of 390 + -20 nm and the second filter 87 of 450-600nm, respectively.
Wherein, fig. 8 is the utility model provides a box-sealing structure schematic diagram of box integrated configuration of three-dimensional nonlinear laser scanning cavity endoscope, as shown in fig. 8, display 55 is integrated together with the box integration of installing each module on the case lid, make things convenient for whole equipment to remove, and change workplace, and this display 55 when using, can place outward on the box, in order to make things convenient for the staff to acquire the information on the display, wherein speculum detecting device 1 in the cavity that objective adjustable among this three-dimensional nonlinear laser scanning cavity endoscope is mouth mirror detecting device. After the three-dimensional nonlinear laser scanning cavity endoscope is used, a worker can carry the equipment box by hand, so that the working place can be conveniently replaced, and the equipment can be more conveniently used particularly in hospitals, laboratories or outdoor places.
Wherein, fig. 9 is the utility model discloses another embodiment provides a box integrated configuration's of three-dimensional nonlinear laser scanning cavity endoscope envelope structure sketch map, as shown in fig. 9, display 55 is integrated together with the box integration of installing each module on the case lid, make things convenient for whole equipment to remove, and change the workplace, and this display 55 when using, can place outward on the box, in order to make things convenient for the staff to acquire the information on the display, wherein interior speculum 1 of the adjustable cavity of objective among this three-dimensional nonlinear laser scanning cavity endoscope is peritoneoscope detecting device, and peritoneoscope detecting device can set up a plurality ofly simultaneously. After the three-dimensional nonlinear laser scanning cavity endoscope is used, a worker can carry the equipment box by hand, so that the working place can be conveniently replaced, and the equipment can be more conveniently used particularly in hospitals, laboratories or outdoor places.
On the basis of above-mentioned each embodiment, the embodiment of the utility model provides an adjustable cavity endoscope detection device of objective among the three-dimensional nonlinear laser scanning cavity endoscope is a plurality of. Namely the embodiment of the utility model provides a fluorescence collection device and fiber coupling module can be simultaneously with the adjustable cavity speculum detecting device fiber communication connection of a plurality of objective, a plurality of detecting device of integration in a three-dimensional nonlinear laser scanning cavity speculum system promptly to the realization is surveyed the different positions of intestines and stomach tissue simultaneously, thereby carries out contrastive analysis.
On the basis of each embodiment, the embodiment of the utility model provides a speculum in three-dimensional nonlinear laser scanning cavity still includes the regulation optic fibre for fluorescence collection device and fiber coupling module respectively with objective adjustable cavity speculum detection device between the optical fiber transmission be connected, wherein:
the length of the adjusting optical fiber can be adjusted. Namely the embodiment of the utility model provides a fluorescence collection device and fiber coupling module in three-dimensional nonlinear laser scanning cavity endoscope carry out fiber transmission through adjustable length regulation optic fibre and adjustable cavity speculum detecting device of objective respectively and are connected, in order to realize according to different experiment scene needs, carry out nimble detection device that removes, avoid limited fiber length's restriction, wherein, the length adjustable who adjusts optic fibre, for the optic fibre through changing different length, realize the application of various occasions, can carry out the optic fibre change of different length as required at any time.
To the three-dimensional nonlinear laser scanning cavity endoscope provided by the above embodiments, the embodiment of the present invention provides another specific implementation manner, fig. 10 is a schematic diagram of a table structure of the three-dimensional nonlinear laser scanning cavity endoscope provided by an embodiment of the present invention, as shown in fig. 10, the three-dimensional nonlinear laser scanning cavity endoscope includes an air extractor 52, a first device 53, a second device 54, a display 55, and a cavity endoscope detection device 1 with an adjustable objective lens, wherein the first device 53 integrates a scanning acquisition controller and an industrial personal computer, the industrial personal computer is electrically connected with the display 55, the second device 54 integrates a femtosecond pulse laser, an optical fiber coupling module and a fluorescence collection device, an illumination module and an imaging module, the optical fiber coupling module and the fluorescence collection device are all connected with an absorption microscope detection device 51 through optical fiber transmission, the objective-adjustable cavity endoscope detection device 1 is a mouth cavity endoscope detection device, and is used for detecting oral tissues of a human body so as to know information such as benign and malignant tumors, infiltration depth, metastasis conditions, and whether cancer residues exist at incisional edges.
Wherein, fig. 11 shows another embodiment of the present invention provides a table-type structural schematic diagram of a three-dimensional nonlinear laser scanning cavity endoscope, as shown in fig. 11, the three-dimensional nonlinear laser scanning cavity endoscope also comprises an air extractor 52, a first device 53, a second device 54, a display 55 and a cavity endoscope detection device 1 with an adjustable objective lens, wherein the first device 53 integrates a scanning acquisition controller and an industrial control computer, the industrial control computer is electrically connected with the display 55, the second device 54 integrates a femtosecond pulse laser, an optical fiber coupling module and a fluorescence collection device, an illumination module and an imaging module, the optical fiber coupling module and the fluorescence collection device are both in optical fiber transmission connection with an absorption microscope detection device 51, wherein the cavity endoscope detection device 1 with an adjustable objective lens is a laparoscope detection device, the laparoscope detection device is embedded in the abdomen of a human body, the gastrointestinal tissues are detected to know the information of malignancy and benign of the tumor, infiltration depth, metastasis condition, whether cancer residue exists at the incisal margin and the like.
Although the present invention has been described with reference to the accompanying drawings, it is not intended to limit the scope of the invention, and it should be understood by those skilled in the art that the above is not required to be limited to the preferred embodiments of the present invention, but rather, the present invention is not limited to the above embodiments. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.
The above-described embodiments of the apparatus are merely illustrative, and the units described as separate parts may or may not be physically separate, and the parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of the present embodiment. One of ordinary skill in the art can understand and implement it without inventive effort.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.
Claims (10)
1. An objective adjustable borescope probe apparatus comprising:
handle casing and detecting tube, handle casing with detecting tube fixed connection, be provided with the detection passageway in the detecting tube, be provided with relay lens, infinite objective and be used for the drive in the detection passageway the zoom motor that infinite objective reciprocated, infinite objective with the zoom motor all is located the access point department of detection passageway, infinite objective the relay lens with the light path structure that sets up in the handle casing forms first light path and second light path, wherein:
the first optical path sequentially comprises a collimating lens, a micro-electromechanical scanning galvanometer, a first lens, a second lens, a dichroic mirror, the relay lens and the infinity objective lens, wherein the collimating lens, the micro-electromechanical scanning galvanometer, the first lens, the second lens, the dichroic mirror, the relay lens and the infinity objective lens are positioned between a general optical fiber interface and the passage port in the handle shell, and the first optical path is used for conducting a laser signal received by the collimating lens from the general optical fiber interface to the passage port;
the second optical path sequentially includes the infinity objective lens, the relay lens and the dichroic mirror between the channel port and the optical fiber universal interface, where the second optical path is used to conduct the optical signal collected by the infinity objective lens from the channel port to the optical fiber universal interface.
2. The objective-adjustable cavity endoscope inspection device according to claim 1, wherein the optical path structure further comprises a liquid lens, the liquid lens is located between the collimating lens and the micro-electromechanical scanning galvanometer to form a new first optical path, and the new first optical path sequentially comprises the collimating lens, the liquid lens, the micro-electromechanical scanning galvanometer, the first lens, the second lens, the dichroic mirror, and the infinity objective lens located between the optical fiber universal interface and the passage port.
3. The objective lens adjustable cavity endoscope detecting device as recited in claim 1 or 2, wherein a plurality of illumination channels are further disposed in the detecting tube, and illumination optical fiber bundles for transmitting illumination light signals are disposed in the illumination channels, wherein the illumination channels are uniformly distributed around the axis of the detecting channel.
4. The objective adjustable borescope probe device of claim 3, further comprising a viewing channel disposed within the probe tube, the viewing channel being located between the probe channel and the illumination channel, wherein:
and an observation lens is arranged at the channel opening of the observation channel and is connected with the bright field optical fiber bundle in the observation channel so as to acquire image information of the tissue area to be detected in front of the infinity objective.
5. The objective lens adjustable cavity endoscope probe of claim 3, wherein an absorption channel is further provided in the probe tube, the absorption channel being located between the illumination channel and the probe tube edge.
6. The objective adjustable cavity endoscope probe of claim 4, wherein said handle housing has a button hole, and a switch button is disposed in said button hole, and said switch button is used to switch different filters to obtain said illumination light signals with different wavelengths.
7. The objective lens adjustable cavity endoscope detecting device of claim 6, characterized in that an imaging button is further disposed in the button hole, and the imaging button is used for controlling an imaging module connected with the bright field fiber bundle to image a tissue region to be detected in front of the infinity objective.
8. A three-dimensional nonlinear laser scanning cavity endoscope is characterized by comprising:
a fluorescence collection device, a scan acquisition controller, a femtosecond pulse laser, a fiber coupling module, and the intracavity scope detection device of any one of claims 1-7, wherein the fluorescence collection device and the fiber coupling module are both in fiber communication with the intracavity scope detection device, and the fluorescence collection device and the intracavity scope detection device are both electrically connected to the scan acquisition controller, wherein:
the femtosecond pulse laser is used for outputting pulse laser signals to the optical fiber coupling module;
the fiber coupling module is used for coupling the pulse laser signal output by the femtosecond pulse laser and transmitting the pulse laser signal to the collimating lens in the cavity endoscope detection device;
the cavity endoscope detection device is used for receiving the pulse laser signal, outputting the pulse laser signal to an autofluorescence substance in a cell of a living body, acquiring a fluorescence signal and a second harmonic signal generated after the autofluorescence substance is excited by the infinity objective lens, and outputting the fluorescence signal and the second harmonic signal to the fluorescence collection device;
the fluorescence collecting device is used for receiving the fluorescence signal and the second harmonic signal and then respectively converting the fluorescence signal and the second harmonic signal into corresponding electric signals;
and the scanning acquisition controller is used for controlling the micro-electromechanical scanning galvanometer to scan the pulse laser signals and synchronously acquire the electric signals.
9. The three-dimensional nonlinear laser scanning cavity endoscope of claim 8, further comprising an illumination module and an imaging module, both of which are in fiber-optic communication connection with the cavity endoscope detection device, wherein:
the illumination module sequentially comprises an illumination lens, a variable optical filter and an illumination light source, the illumination lens is connected with the illumination optical fiber bundle, and the illumination light source is used for providing an illumination light signal;
the imaging module sequentially comprises an imaging lens and a camera, the imaging lens is connected with the bright field optical fiber bundle, and the camera is used for acquiring image information of a tissue area to be detected.
10. The three-dimensional nonlinear laser scanning cavity endoscope in accordance with claim 8, wherein the fluorescence collecting device comprises a collecting fiber optic universal interface, a first photomultiplier tube, a second photomultiplier tube, and a first collecting light path between the collecting fiber optic universal interface and the first photomultiplier tube, a second collecting light path between the collecting fiber optic universal interface and the second photomultiplier tube, wherein:
the first collecting light path sequentially comprises a coupling collecting lens, an infrared filter, a first dichroic mirror, a first filter and a first collecting lens, wherein the first collecting light path is used for collecting the fluorescent signals received by the fluorescent collecting device, and the first photomultiplier is used for converting the fluorescent signals into first electric signals;
the second collecting light path sequentially comprises the coupling collecting lens, the infrared filter, the first dichroic mirror, the second filter and the second collecting lens, wherein the second collecting light path is used for collecting the second harmonic signals received by the fluorescence collecting device, and the second photomultiplier is used for converting the second harmonic signals into second electric signals.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109674438A (en) * | 2019-01-31 | 2019-04-26 | 北京超维景生物科技有限公司 | The adjustable cavity endoscope detection device of object lens and laser scanning cavity endoscope |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109674438A (en) * | 2019-01-31 | 2019-04-26 | 北京超维景生物科技有限公司 | The adjustable cavity endoscope detection device of object lens and laser scanning cavity endoscope |
| CN109674438B (en) * | 2019-01-31 | 2024-02-27 | 北京超维景生物科技有限公司 | Objective lens adjustable cavity endoscope detection device and laser scanning cavity endoscope |
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