CN109656014A - Multichannel phosphor collection device and three dimensional non-linear laser scanning cavity endoscope - Google Patents
Multichannel phosphor collection device and three dimensional non-linear laser scanning cavity endoscope Download PDFInfo
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Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B23/00—Telescopes, e.g. binoculars; Periscopes; Instruments for viewing the inside of hollow bodies; Viewfinders; Optical aiming or sighting devices
- G02B23/24—Instruments or systems for viewing the inside of hollow bodies, e.g. fibrescopes
- G02B23/2407—Optical details
- G02B23/2461—Illumination
- G02B23/2469—Illumination using optical fibres
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/04—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances
- A61B1/05—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances characterised by the image sensor, e.g. camera, being in the distal end portion
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/06—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements
- A61B1/07—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements using light-conductive means, e.g. optical fibres
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B23/00—Telescopes, e.g. binoculars; Periscopes; Instruments for viewing the inside of hollow bodies; Viewfinders; Optical aiming or sighting devices
- G02B23/24—Instruments or systems for viewing the inside of hollow bodies, e.g. fibrescopes
- G02B23/2476—Non-optical details, e.g. housings, mountings, supports
- G02B23/2484—Arrangements in relation to a camera or imaging device
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- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
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Abstract
The embodiment of the present invention provides a kind of multichannel phosphor collection device and three dimensional non-linear laser scanning cavity endoscope.Wherein, above-mentioned multichannel phosphor collection device includes binary channels phosphor collection module, illumination Multiplexing module and imaging Multiplexing module, after two-photon fluorescence signal and second harmonic signal that wherein binary channels phosphor collection module synchronization convergence illumination Multiplexing module and imaging Multiplexing module are collected into, corresponding electric signal is converted to.Multichannel phosphor collection device and three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention uses modular combination, pass through illumination Multiplexing module and the optical fiber multiplexing function being imaged in Multiplexing module, two-photon fluorescence signal and second harmonic signal before acquiring distal endoscope detection device, and it synchronizes and converges to binary channels phosphor collection module, so that binary channels phosphor collection module collection major part two-photon fluorescence signal and second harmonic signal, to realize more accurate eucaryotic cell structure imaging.
Description
Technical field
The present embodiments relate to laser scanning endoscopic technique field more particularly to a kind of multichannel phosphor collection device and
Three dimensional non-linear laser scanning cavity endoscope.
Background technique
Gastrointestinal cancer is to induce the second largest reason of developed country crowd cancer stricken death, and in recent years should
Trend is more and more obvious.Surgery radical operation is mainly used for the treatment of gastrointestinal cancer, but is embodied outer
When section's radical operation it needs to be determined that the operation excision specific range, therefore carry out operation consent, it is to be understood that tumour
Whether there is or not Cancer residuals etc. for good pernicious, invasive depth, transfer case and incisxal edge.Therefore biopsy is swollen for gastrointestinal tract under preoperative Gastrointestinal Endoscopes
Tumor tissue diagnosis is a critically important diagnostic evidence.And according to tumorous size, growth position, invasive depth etc., by gastric cancer
Art formula be divided into that stomach is cut entirely, stomach time is cut entirely, gastric resection and endoscopic inferior mucosa or submucous resection etc..
And it is newest at present for carrying out eucaryotic cell structure detection to gastrointestinal tissue, oral cavity and uterine cavity inner tissue in human abdominal cavity
The Gastrointestinal Endoscopes of imaging have the cavity endoscope based on two photon imaging technology, but are all mostly by the object lens in detection device
After being acquired to two-photon signal, single optical path processing is carried out by the phosphor collection device on backstage.
Single two-photon processing optical path is only integrated in above-mentioned phosphor collection device, function is excessively single, is unable to satisfy
The demand of multichannel collection fluorescence signal.
Summary of the invention
For the technical problems in the prior art, the embodiment of the present invention provides a kind of multichannel phosphor collection device and three
It ties up non-linear laser and scans cavity endoscope.
In a first aspect, the embodiment of the present invention provides a kind of multichannel phosphor collection device, comprising:
Binary channels phosphor collection module, illumination Multiplexing module and imaging Multiplexing module, the illumination Multiplexing module and institute
Imaging Multiplexing module is stated to connect with the binary channels phosphor collection module fiber optic communication, in which:
The illumination Multiplexing module for providing illumination optical signal for distal endoscope detection device, and is based on illumination light
After fine beam collects two-photon fluorescence signal and second harmonic signal, the two-photon fluorescence signal and second harmonic letter are transmitted
Number to the binary channels phosphor collection module；
The imaging Multiplexing module, for before distal endoscope detection device object lens test serum region carry out at
Picture, and after collecting two-photon fluorescence signal and second harmonic signal based on light field fiber optic bundle, transmit the two-photon fluorescence signal
With the second harmonic signal to the binary channels phosphor collection module；
The binary channels phosphor collection module, it is glimmering for collecting two-photon based on the object lens in distal endoscope detection device
Optical signal and second harmonic signal, and synchronize described pair that the convergence illumination Multiplexing module and the imaging Multiplexing module transmit
After photon fluorescence signal and the second harmonic signal, the two-photon fluorescence signal and the second harmonic signal are converted as phase
The electric signal answered.
Second aspect, the embodiment of the present invention provide a kind of three dimensional non-linear laser scanning cavity endoscope, comprising:
Cavity endoscope detection device, scanning collection controller, femtosecond pulse laser, fiber coupling module, Yi Jiben
The multichannel phosphor collection device that inventive embodiments first aspect provides, the multichannel phosphor collection device and the fiber coupling mould
Block is connect with the zoom-type cavity endoscope detection device fiber optic communication, the multichannel phosphor collection device and the cavity
Endoscope detection device is electrically connected with the scanning collection controller, in which:
The femtosecond pulse laser, for exporting pulsed laser signal to the fiber coupling module；
The fiber coupling module, for coupling the pulsed laser signal of the femtosecond pulse laser output, and
Transmit collimation lens of the pulsed laser signal into the cavity endoscope detection device；
The cavity endoscope detection device exports the pulse laser letter after receiving the pulsed laser signal
Number autofluorescence substance intracellular to life entity, and obtain the fluorescence signal generated after the autofluorescence substance excitation and
Second harmonic signal, and the fluorescence signal and the second harmonic signal are exported to the phosphor collection device；
The scanning collection controller, for controlling the micro electromechanical scanning galvanometer pair in the cavity endoscope detection device
The pulsed laser signal be scanned and synchronous acquisition described in electric signal.
Multichannel phosphor collection device and three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention uses
Modular combination passes through illumination Multiplexing module and the optical fiber multiplexing function being imaged in Multiplexing module, acquisition distal endoscope detection
Two-photon fluorescence signal and the second harmonic signal before device, and synchronize and converge to binary channels phosphor collection module, so that
Binary channels phosphor collection module can be collected into most of two-photon fluorescence signal before distal endoscope detection device and described
Second harmonic signal, to realize more accurate eucaryotic cell structure imaging.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is this hair
Bright some embodiments for those of ordinary skill in the art without creative efforts, can be with root
Other attached drawings are obtained according to these attached drawings.
Fig. 1 is multichannel phosphor collection apparatus structure schematic diagram provided in an embodiment of the present invention；
Fig. 2 is the binary channels phosphor collection modular structure in multichannel phosphor collection device provided in an embodiment of the present invention
Schematic diagram；
Fig. 3 is the three dimensional non-linear laser scanning cavity endoscope structure schematic diagram that one embodiment of the invention provides；
Fig. 4 be another embodiment of the present invention provides three dimensional non-linear laser scanning cavity endoscope structure schematic diagram；
Fig. 5 is the cavity endoscope detection device structural schematic diagram that one embodiment of the invention provides；
Fig. 6 be another embodiment of the present invention provides cavity endoscope detection device structural schematic diagram；
Fig. 7 is the three dimensional non-linear laser scanning cavity endoscope structure schematic diagram that yet another embodiment of the invention provides；
Fig. 8 is the box composite structure for the three dimensional non-linear laser scanning cavity endoscope that one embodiment of the invention provides
Joint sealing structural schematic diagram；
Fig. 9 be another embodiment of the present invention provides three dimensional non-linear laser scanning cavity endoscope box composite structure
Joint sealing structural schematic diagram；
Figure 10 provides the mesa structure schematic diagram of three dimensional non-linear laser scanning cavity endoscope for one embodiment of the invention；
Another embodiment of the present invention provides the mesa structure schematic diagrames of three dimensional non-linear laser scanning cavity endoscope by Figure 11.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art
Every other embodiment obtained without creative efforts, shall fall within the protection scope of the present invention.
At present based on Gastrointestinal Endoscopes, iconography imaging is carried out supplemented by CT, MRI etc. to obtain the good pernicious, leaching of tumour
Moisten depth, transfer case and incisxal edge whether there is or not relevant informations such as Cancer residuals, have the shortcomings that in concrete operations, for example is easy
Lead to intestinal tube or knurl bleeding, need artificial drawing or squeeze, when Gastrointestinal Endoscopes cannot pass through intestinal tube, carries out endoscopic biopsy repeatedly
So that time delay, if causing severe haemorrhage also needs additional first aid hemostasis etc..And the complementary detection methods such as CT, MRI,
The invasive depth and lymphatic metastasis situation of upper gastrointestinal road tumour are unable to judge accurately in clinical practice.And by ultrasound
Mirror judges gastroenteric tumor T by stages, its accuracy of document report is only 44.7%~78%, and being not enough to, which becomes one, reliably examines
Disconnected standard, and endoscopic ultrasonography is ineffective to the preoperative judge of local resection operation, can not precisely subdivided gastrointestinal mucosa level, and
To N, effect is also poor by stages.
And traditional white light laparoscope can assess many gastrointestinal diseases with endoscope, but the technology is only limitted to examine
Survey general form variation.Although being easy to find suspicious region, compared with In vivo detection technology, these technologies and false positive rate
And specificity etc. is related.White light endoscopy is associated with the extensive error that micro-variations diagnose, including ulcerative colitis
Or the inspection diagnosis including Barrett oesophagus and Flat Adenoma depauperation.Confocal endoscope combination laser technology, fluorescence are visited
Survey technology, fast scanning techniques etc. are because that can detect mucous membrane variation in microscopic scale, it is possible to for replacing tissue biopsy, and by
To extensive concern, which has highly sensitive and specificity.But be copolymerized burnt based endoscopic imaging technology still by
As the limitation of depth and fluorescent dye, since stomach and intestine sample has very strong absorption and scattering, imaging depth only to exist visible light
Superficial layer, and it also requires injecting specific fluorescent staining developer, operation is excessively complicated, cannot accurately obtain the leaching of tumour
Moistening depth, transfer case and surgical operation incisxal edge, whether there is or not the relevant informations such as Cancer residual.
And two-photon micro-imaging technique uses the longer femtosecond pulse laser of wavelength to have imaging as excitation light source
The features such as depth is deep, light injury is small, photobleaching region is small, phosphor collection is high-efficient, has in the imaging deep to biological tissue
There is epoch-making meaning.It is micro- that the W.Denk et al. of nineteen ninety Cornell University has developed First two-photon fluorescence in the world
Mirror, using the multi-photon micro-imaging technique based on nonlinear optics and femtosecond pulse.The technology is by utilizing living body
The second harmonic that the autofluorescence and collagen tissue that cell itself generates in tissue generate, can obtain sample real-time, quickly
Institutional framework and cellular morphology.Early in 1986, second harmonic was used for skin research and the research of coronary artery micro-imaging, card
Its real feasibility that be used to observe biological tissue.MPM also can be used as an important tool of cancer research.Cell itself produces
Raw autofluorescence from intracellular nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) (FAD),
It is 460nm that NADH, which issues wavelength, and the secondary oscillation harmonic wave of collagen is 370~390nm, so leading to when observing tumor specimen tissue
Often select the multiphoton microscope of 780~940nm range.MPM imaging is not only suitable with the tumor tissue pathology of standard, simultaneously
The additional information of tumor neogenetic process is also provided, such as can reflect the metabolism of tumor tissue cell by surveying rate value NADH/FAD
It is horizontal.
Using multi-photon imaging technique, multiphoton microscope is capable of providing real-time stomach intestinal tissue's structure and cellular morphology
Learn information.Multi-photon imaging technique has without exogenous marker tissue, extremely sensitive to collagen, small to the light injury of tissue and wear
The features such as depth is deep thoroughly, may be applied to the optical biopsy of gastroenteric tumor.There is presently no useful clinically two-photon abdomens
Hysteroscope and endoscope, and the cavity endoscope detection device based on two photon imaging, with real-time detection stomach intestinal tissue in situ
Information.
It is newest at present for gastrointestinal tissue in human abdominal cavity, oral cavity and uterine cavity inner tissue carry out eucaryotic cell structure detection at
The Gastrointestinal Endoscopes of picture have the cavity endoscope based on two photon imaging technology, but are all mostly by the object lens pair in detection device
After two-photon signal is acquired, single optical path processing is carried out by the phosphor collection device on backstage.In above-mentioned phosphor collection device
It is only integrated with single two-photon processing optical path, function is excessively single, is unable to satisfy the demand that multichannel collects fluorescence signal.
In order to realize to the collection of the multichannel of fluorescence signal, the embodiment of the invention provides a kind of multichannel phosphor collection device,
Fig. 1 is multichannel phosphor collection apparatus structure schematic diagram provided in an embodiment of the present invention, as shown in Figure 1, the device includes:
Binary channels phosphor collection module 11, illumination Multiplexing module 12 and imaging Multiplexing module 13, the illumination are multiplexed mould
Block 12 and the imaging Multiplexing module 13 are connect with 11 fiber optic communication of binary channels phosphor collection module, in which:
The illumination Multiplexing module 12, for providing illumination optical signal for distal endoscope detection device, and based on illumination
After fiber optic bundle collects two-photon fluorescence signal and second harmonic signal, the two-photon fluorescence signal and the second harmonic are transmitted
Signal is to the binary channels phosphor collection module；
The imaging Multiplexing module 13, for before distal endoscope detection device object lens test serum region carry out at
Picture, and after collecting two-photon fluorescence signal and second harmonic signal based on light field fiber optic bundle, transmit the two-photon fluorescence signal
With the second harmonic signal to the binary channels phosphor collection module；
The binary channels phosphor collection module 11, for collecting two-photon based on the object lens in distal endoscope detection device
Fluorescence signal and second harmonic signal, and the synchronous convergence illumination Multiplexing module 12 and the imaging Multiplexing module 13 transmit
After the two-photon fluorescence signal and the second harmonic signal, the two-photon fluorescence signal and second harmonic letter are converted
Number be corresponding electric signal.
Specifically, multichannel phosphor collection device provided in an embodiment of the present invention mainly includes three modules, respectively bilateral
Road phosphor collection module 11, illumination Multiplexing module 12 and imaging Multiplexing module 13, illuminate Multiplexing module 12 and the imaging is multiple
It is connect with 11 fiber optic communication of binary channels phosphor collection module with module 13, illuminates Multiplexing module 12 and imaging multiplexing
Module 13 can include two-photon fluorescence signal and second harmonic signal with collecting part fluorescence signal, meanwhile, illumination multiplexing mould
Block 12, which also has, provides the function of illumination optical signal for distal endoscope detection device, and imaging Multiplexing module 13 also has to distal end
The function that test serum region before endoscope detection device object lens is imaged, illumination Multiplexing module 12 and imaging Multiplexing module
Collected two-photon fluorescence signal and second harmonic signal are transferred to binary channels phosphor collection module 11 by optical fiber by 13, double
Channel fluorescence collection module 11 collects two-photon fluorescence signal and secondary humorous self by object lens in distal endoscope detection device
Wave signal, and synchronous convergence illumination Multiplexing module 12 and the imaging collected two-photon fluorescence signal of Multiplexing module 13 and secondary humorous
Wave signal converts the two-photon fluorescence signal and the second harmonic signal as corresponding electric signal, passes through telecommunications to realize
Number corresponding eucaryotic cell structure image is obtained, i.e. binary channels phosphor collection module 11 will illuminate Multiplexing module 12 and imaging Multiplexing module
13 transmission two-photon fluorescence signals and self by object lens acquire two-photon fluorescence signal pool all the way, conversion convergence after
Two-photon fluorescence signal be the first electric signal, binary channels phosphor collection module 11 will illuminate Multiplexing module 12 and imaging multiplexing mould
The second harmonic signal and pool another way self by the second harmonic signal that object lens acquire that block 13 transmits, after conversion convergence
Second harmonic signal be the second electric signal, wherein illumination Multiplexing module passes through illumination fiber optic bundle and distal endoscope detection device
Fiber optic communication connection, provides illumination optical signal, and synchronous collection distal endoscope detection device for distal endoscope detection device
Preceding two-photon fluorescence signal and second harmonic signal；Multiplexing module is imaged to fill by light field fiber optic bundle and distal endoscope detection
Fiber optic communication connection is set, the test serum region before distal endoscope detection device object lens is imaged, and synchronous collection is remote
Two-photon fluorescence signal and second harmonic signal before holding endoscope detection device.
Multichannel phosphor collection device provided in an embodiment of the present invention use modular combination, by illumination Multiplexing module and at
As the optical fiber multiplexing function in Multiplexing module, two-photon fluorescence signal before acquiring distal endoscope detection device and described secondary
Harmonic signal, and synchronize and converge to binary channels phosphor collection module, so that multichannel phosphor collection device is received relative to existing fluorescence
Optical fiber single channel is collected by object lens in acquisition means and collects two-photon fluorescence signal and second harmonic signal, can be collected into distal end
Before sight glass detection device, more two-photon fluorescence signals and the second harmonic signal, and pass through binary channels phosphor collection mould
Block converts two-photon fluorescence signal and second harmonic signal as corresponding electric signal, with realize more accurate eucaryotic cell structure at
Picture.
Illumination on the basis of the various embodiments described above, in multichannel phosphor collection device provided in an embodiment of the present invention
Multiplexing module includes that optical path is collected in illumination path and the first multiplexing, as shown in Figure 1, in which:
Institute's illumination path successively includes that illumination fiber optic bundle 121, first is multiplexed dichroscope 122, variable filter 123, shines
Bright lens 124 and lighting source 125；
It successively includes that the multiplexing dichroscope 122, first of illumination fiber optic bundle 121, first is multiple that optical path is collected in first multiplexing
With collecting lens 126 and the first transmission fiber 127.It is described in multichannel phosphor collection device i.e. provided in an embodiment of the present invention
Illumination Multiplexing module has the function of illumination and phosphor collection, and corresponding optical path is that optical path is collected in illumination path and the first multiplexing,
In, illumination path include illumination fiber optic bundle 121, first be multiplexed dichroscope 122, variable filter 123, illuminating lens 124 with
And lighting source 125, lighting source 125 launch illumination optical signal to illuminating lens 124, converge to variable filter 123, pass through
After filtering, transmission the first multiplexing dichroscope 122 is transmitted to distal endoscope detection device through illumination fiber optic bundle 121, peeps to interior
Mirror detection device provides illumination, meanwhile, it successively includes that illumination fiber optic bundle 121, first is multiplexed dichroic that optical path is collected in the first multiplexing
Mirror 122, first is multiplexed collecting lens 126 and the first transmission fiber 127, and illumination fiber optic bundle 121 acquires distal endoscope detection
Two-photon fluorescence signal and the second harmonic signal before device are simultaneously transferred to the first multiplexing dichroscope 122, multiple through first
The first multiplexing collecting lens 126 are reflexed to dichroscope 122, convergence closes beam to the first transmission fiber 127, through the first transmission light
Fibre 127 is transmitted to binary channels phosphor collection module, improves phosphor collection efficiency, thus achieve the purpose that improve signal noise ratio (snr) of image,
Wherein, lighting source 125 passes through 123 runner of electric variable optical filter, can switch different optical filters, to obtain different wave length
Illumination optical signal, basic principle is not interfere two-photon fluorescence imaging, for example obtain autofluorescence and when second harmonic,
Red or infrared optical filter can be switched to, to obtain the illumination light letter of 370nm, 635nm or infrared 850nm, 940nm
Number, illumination optical signal enters illumination fiber optic bundle 121 by Lens Coupling.
Imaging on the basis of the various embodiments described above, in multichannel phosphor collection device provided in an embodiment of the present invention
Multiplexing module includes that optical path is collected in imaging optical path and the second multiplexing, as shown in Figure 1, in which:
The imaging optical path successively include light field fiber optic bundle 131, second be multiplexed dichroscope 132, imaging len 133 and
Camera 134；
It successively includes that the multiplexing dichroscope 132, second of light field fiber optic bundle 131, second is multiple that optical path is collected in second multiplexing
With collecting lens 135 and the second transmission fiber 136.It is described in multichannel phosphor collection device i.e. provided in an embodiment of the present invention
Imaging Multiplexing module has the function of imaging and phosphor collection, and corresponding optical path is that optical path is collected in imaging optical path and the second multiplexing,
Wherein, the imaging optical path successively includes that light field fiber optic bundle 131, second is multiplexed dichroscope 132, imaging len 133 and phase
Machine 134, camera 134 is multiplexed dichroscope 132 by light field fiber optic bundle 131, second, imaging len 133 captures distal endoscope
Test serum area information before detection device object lens passes through the optical fiber multiplexing function of light field fiber optic bundle 131, light field fiber optic bundle
Two-photon fluorescence signal before 131 acquisition distal endoscope detection devices and the second harmonic signal and it is transferred to the second multiplexing
Dichroscope 132 reflexes to the second multiplexing collecting lens 135 through the second multiplexing dichroscope 132, converges to the second transmission fiber
136, it is transmitted to binary channels phosphor collection module through the second transmission fiber 136, wherein camera 134 can be two and binocular bright field light
Fine beam 131 is corresponding, light field imaging and the multi-modal laparoscope of two photon imaging composition, light field binocular three-dimensional stereo laparoscope mode,
Carry out wide-field sample view, the basic pattern of main detection sample.For there is suspicious or interested region, can switch to
Two-photon mode carries out autofluorescence and Second Harmonic Imaging, observes the cell grade form of sample, provides for further judgement
Foundation, wherein camera 134 can be the imaging device based on image devices such as CCD or CMOS.
On the basis of the various embodiments described above, Fig. 2 is the institute in multichannel phosphor collection device provided in an embodiment of the present invention
Binary channels phosphor collection modular structure schematic diagram is stated, as shown in Fig. 2, in multichannel phosphor collection device provided in an embodiment of the present invention
The binary channels phosphor collection module include that object lens collect optical fiber, fiber optic universal interface 881, the first photomultiplier tube 882, the
Two photomultiplier tubes 883 and between fiber optic universal interface 881 and the first photomultiplier tube 882 first collect optical path,
Second between fiber optic universal interface 881 and the second photomultiplier tube 883 collects optical path, in which:
First transmission fiber, second transmission fiber and the object lens collect optical fiber with the fiber optic universal
Interface Fiber communication connection；
First collection optical path successively includes coupled lens 81, infrared fileter 82, the first dichroscope 83, the first optical filter
84 and first collecting lens 85, wherein the first collection optical path is for collecting the fluorescence signal that phosphor collection device receives, and the
One photomultiplier tube 882 is the first electric signal for conversion fluorescence signal；
Second collection optical path successively includes coupled lens 81, infrared fileter 82, the first dichroscope 83, the second dichroic
Mirror 86, the second optical filter 87 and the second collecting lens 88, wherein the second collection optical path is for collecting the reception of phosphor collection device
The second harmonic signal arrived, the second photomultiplier tube 883 is for converting second harmonic signal as the second electric signal.I.e. the present invention is real
The binary channels phosphor collection module in the multichannel phosphor collection device of example offer is provided and is integrated with two paths of signals collection optical path, point
Not Wei fluorescence signal collection optical path and second harmonic signal collect optical path, collect respectively and converted illumination Multiplexing module, imaging be multiple
It is the first electric signal and illumination multiplexing mould with the two-photon fluorescence signal that module and binary channels phosphor collection module collection arrive
The second harmonic that block, imaging Multiplexing module and binary channels phosphor collection module collection arrive is the second electric signal, wherein it is handled
Fluorescence signal and second harmonic signal in distal endoscope detection device object lens acquire fluorescence signal and second harmonic
It signal and illumination Multiplexing module and Multiplexing module is imaged converges to fluorescence signal in binary channels phosphor collection module and secondary
Harmonic signal.
Wherein, first collect optical path in the first dichroscope 83 be transillumination Multiplexing module, imaging Multiplexing module with
And the two-photon fluorescence signal that binary channels phosphor collection module collection arrives, indirect illumination Multiplexing module, imaging Multiplexing module and
The dichroscope for the second harmonic that binary channels phosphor collection module collection arrives, the second dichroscope 86 and the first dichroscope 83 are
Same dichroscope, is used for reflected second harmonics, and the first optical filter 84 filters out remaining interference letter for transmiting fluorescence signal
Number, the second optical filter 87 filters out remaining interference signal for transmiting corresponding second harmonic signal, for example, flying using 780nm
When autofluorescence substance in second optical fiber laser exciting human abdominal cavity or Stomatocyte, the second harmonic letter of 390nm can be obtained
Number and 450-600nm two-photon auto flourescence signals, passed through by 420nm above wavelength, 420 or less wavelength reflections two to
Look mirror i.e. the first dichroscope 83 can separate two-way fluorescence, use the first optical filter 84 and 450- of 390 ± 20nm respectively
The second optical filter 87 available clean second harmonic signal and fluorescence signal of 600nm, wherein object lens collect optical fiber and remote
Object lens in the endoscope detection device of end are connected, to transmit the two-photon fluorescence signal being collected into and second harmonic signal to light
Fine general-purpose interface.
Illumination on the basis of the various embodiments described above, in multichannel phosphor collection device provided in an embodiment of the present invention
Multiplexing module is multiple.Multiple illumination multiplexing moulds can be set in multichannel phosphor collection device i.e. provided in an embodiment of the present invention
Block collects fluorescence signal and second harmonic signal to realize to greatest extent.
Imaging on the basis of the various embodiments described above, in multichannel phosphor collection device provided in an embodiment of the present invention
Multiplexing module is multiple.Multiple imaging multiplexing moulds can be set in multichannel phosphor collection device i.e. provided in an embodiment of the present invention
Block collects fluorescence signal and second harmonic signal to realize to greatest extent.
On the basis of the various embodiments described above, multichannel phosphor collection device provided in an embodiment of the present invention, as shown in Figure 1,
It further include industrial personal computer 14, the binary channels phosphor collection module is connect with 14 fiber optic communication of industrial personal computer, the illumination multiplexing
Module and the imaging Multiplexing module are with the industry control mechatronics, and the industrial personal computer 14 is for obtaining the first electric signal and the
Two electric signals, and the first fluorescent image is generated based on the first electric signal and the second fluorescent image is generated based on the second electric signal.
The collected fluorescence signal of multichannel phosphor collection device i.e. provided in an embodiment of the present invention and second harmonic signal rotate into correspondence
The first electric signal and the second electric signal after, obtained by industrial personal computer 14 and generate corresponding first fluorescent image and the second fluorescence
Image, the industrial personal computer are based on the first electric signal and generate the first fluorescent image and generate the second fluorogram based on the second electric signal
Picture can be respectively used to display eucaryotic cell structure and fibre structure information, control software is wherein equipped on industrial personal computer, soft by controlling
Part sends control instruction to scanner, to control scanning collection controller, to obtain above-mentioned first electric signal and the second telecommunications
Number.
The embodiment of the present invention also provides a kind of three dimensional non-linear laser scanning cavity endoscope, and Fig. 3 is that the present invention one is implemented
The three dimensional non-linear laser scanning cavity endoscope structure schematic diagram that example provides, as shown in figure 3, the three dimensional non-linear laser scanning
Cavity endoscope includes:
Cavity endoscope detection device 18, scanning collection controller 15, femtosecond pulse laser, fiber coupling module, with
And the multichannel phosphor collection device that the various embodiments described above provide, the multichannel phosphor collection device and the fiber coupling module are equal
It is connect with 18 fiber optic communication of cavity endoscope detection device, the multichannel phosphor collection device and the cavity endoscope are visited
Device 18 is surveyed to be electrically connected with the scanning collection controller 15, in which:
The femtosecond pulse laser, for exporting pulsed laser signal to the fiber coupling module；
The fiber coupling module, for coupling the pulsed laser signal of the femtosecond pulse laser output, and
Transmit collimation lens of the pulsed laser signal into the cavity endoscope detection device 18；
The cavity endoscope detection device 18 exports the pulse laser after receiving the pulsed laser signal
The signal autofluorescence substance intracellular to life entity, and obtain the fluorescence signal generated after the autofluorescence substance excitation
And second harmonic signal, and the fluorescence signal and the second harmonic signal are exported to the phosphor collection device；
The scanning collection controller 15, for controlling the vibration of the micro electromechanical scanning in the cavity endoscope detection device 18
Mirror to the pulsed laser signal be scanned and synchronous acquisition described in electric signal.
Specifically, three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention includes that cavity endoscope is visited
Survey what device 18, scanning collection controller 15, femtosecond pulse laser, fiber coupling module and the various embodiments described above provided
Multichannel phosphor collection device detects human body gastrointestinal tissue and oral cavity tissue using two photon imaging technology to be formed
Three dimensional non-linear laser scanning cavity endoscope, wherein femtosecond pulse laser can be with emission pulse laser signal for swashing
The autofluorescence substance in human body gastrointestinal tissue and oral tissue cell is sent out, multiphoton fluorescence signal and second harmonic letter are generated
Number, FAD and collagen in the femtosecond pulse laser activated cell including using 920nm excite the fluorescence of 500-600nm
The second harmonic signal of signal and 460nm, and pass through 780nm femtosecond pulse laser activated cell in FAD or NADH
Equal autofluorescences substance, to generate corresponding fluorescence signal and second harmonic signal, wherein femtosecond pulse laser and optical fiber coupling
Molding block is grouped together into laser emitting module；
Wherein, multichannel phosphor collection device includes binary channels phosphor collection module 11, illumination Multiplexing module 12 and imaging
Multiplexing module 13 is integrated with two paths of signals and collects optical path, and respectively fluorescence signal collection optical path and second harmonic signal collects light
The collection respectively of road, Lai Shixian fluorescence signal and second harmonic signal；Scanning collection controller 15 controls micro electromechanical scanning galvanometer
Pulsed laser signal is scanned and autofluorescence substance is excited to generate fluorescence signal and second harmonic signal, and is acquired glimmering
The first electric signal and the second electric signal that light collecting device conversion fluorescence signal and second harmonic signal obtain；The three dimensional non-linear
Laser scanning cavity endoscope can be divided into laparoscope and mouth mirror as schemed according to the difference of 18 structure of cavity endoscope detection device
Shown in 3, cavity endoscope detection device 18 is laparoscope detection device, then the three dimensional non-linear laser scanning cavity endoscope is
Laparoscope.Fig. 4 be another embodiment of the present invention provides three dimensional non-linear laser scanning cavity endoscope structure schematic diagram, such as scheme
Shown in 4, cavity endoscope detection device 18 is mouth mirror detection device, then the three dimensional non-linear laser scanning cavity endoscope is
Mouth mirror.Wherein, the resolution ratio of the three dimensional non-linear laser scanning cavity endoscope may be configured as 800nm, and visual field can be
400 microns * 400 microns, image taking speed can be 26 frames (256*256 pixel) or 13 frames (512*512 pixel).
Three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention uses multichannel phosphor collection device, sweeps
Acquisition controller, femtosecond pulse laser, fiber coupling module and cavity endoscope detection device are retouched, to be formed using double
The laser scanning cavity endoscope that photon imaging technology detects human body gastrointestinal tissue and oral cavity tissue, by using more
Binary channels phosphor collection module, illumination Multiplexing module and imaging Multiplexing module visit cavity endoscope in the phosphor collection device of road
The fluorescence signal and second harmonic signal generated after cell activation before survey device carries out multichannel collection, and is accordingly imaged, and obtains
Corresponding cell tissue structural information is obtained, equipment operation is simple, easy to use.
On the basis of the various embodiments described above, three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention
In the cavity endoscope detection device include that outer fixed shell and interior clamping device, Fig. 5 provide for one embodiment of the invention
Cavity endoscope detection device structural schematic diagram, as shown in figure 5, the interior clamping device 182 is set to the outer set casing
In body 181, it is provided with zoom motor on the inside of the outer fixed shell 181, to drive the interior clamping device 182 relatively outer solid
Fixed shell 181 moves up and down, and is provided with the optical path knot for being used to form the first optical path and the second optical path in the interior clamping device 182
Structure, in which:
First optical path successively includes collimation lens 1821, micro electromechanical scanning galvanometer 1822, lens 1823, dichroscope
1824, relay lens 1825 and object lens 1826, wherein first optical path is for conducting, the collimation lens 1821 is received to swash
Optical signal is from the collimation lens 1821 to the object lens 1826；
Second optical path successively includes the object lens 1826, the relay lens 1825 and the dichroscope 1824,
Wherein second optical path is for conducting the collected optical signal of the object lens 1826 from the object lens 1826 to the dichroic
Mirror 1824.Cavity endoscope detection in three dimensional non-linear laser scanning cavity endoscope i.e. provided in an embodiment of the present invention
Device includes 182 two primary structures of outer fixed shell 181 and interior clamping device, wherein interior clamping device 182 is an entirety,
It is built-in with transmission laser signal and collects the optical path of two-photon signal and second harmonic signal, respectively the first optical path and the second light
Road, the first optical path include collimation lens 1821, micro electromechanical scanning galvanometer 1822, lens, dichroscope, relay lens 1825 and object
Mirror 1826 passes through the first light for the laser signal of autofluorescence substance in exciting human gastrointestinal tissue or oral tissue cell
After collimation lens 1821, micro electromechanical scanning galvanometer 1822, lens, dichroscope, relay lens 1825 and object lens 1826 in road,
It is emitted on autofluorescence substance from object lens 1826, excitation autofluorescence substance generates two-photon signal and second harmonic signal
Afterwards, two-photon signal and second harmonic signal are collected by object lens 1826, and by the relay lens 1825 and two in the second optical path
To Look mirror, it is collected into for obtaining the detection information of gastrointestinal tissue or oral cavity tissue to be measured in phosphor collection device, it is swollen to judge
Whether there is or not the relevant informations such as Cancer residual for invasive depth, transfer case and the surgical operation incisxal edge of tumor, wherein passing through outer fixed shell
The zoom motor that 181 insides are arranged can be to entire interior clamping device 182 including the first optical path and the second optical path in device
Light channel structure move up and down, with realize different depth histocyte detection, obtain different depth eucaryotic cell structure letter
Breath.
Wherein, the setting of relay lens 1825 is conducted for long range for exciting autofluorescence substance in 1826 inside of object lens
Laser signal from dichroscope to object lens 1826, and the conduction collected two-photon signal of object lens 1826 and second harmonic
Signal to dichroscope, the image planes of laser signal object lens 1826 are overlapped with the focal plane of relay lens 1825, will pass through micro electromechanical scanning
The laser signal scanning area of mirror with ratio of 1:1 etc. than conduction at the image planes of object lens 1826, wherein relay lens 1825 can be with
Extended according to specific needs or is shortened.
Wherein, dichroscope 1824, which can according to need to be set as growing, leads to short anti-dichroscope or short elongated anti-dichroic
Mirror transmits the pulsed laser signal for exciting autofluorescence substance, reflecting and collecting arrives when that is, setting length leads to short anti-dichroscope
Two-photon signal and second harmonic signal, at this point, the varifocal cavity endoscope detection device can be laparoscope detection
Device；When the dichroscope 1824 is short elongated anti-dichroscope, the pulse laser for exciting autofluorescence substance is reflected
Signal, transmits the two-photon signal and second harmonic signal being collected into, and dichroscope reflection is by collimation lens 1821, micro-
The laser signal on dichroscope is incident on after electromechanical scanning galvanometer 1822, lens by relay lens 1825 to object lens 1826, thoroughly
The collected two-photon signal of object lens 1826 and second harmonic signal are penetrated, at this point, the varifocal cavity endoscope detection device
It can be the detection device of mouth mirror.
Optical path knot on the basis of the various embodiments described above, in cavity endoscope detection device provided in an embodiment of the present invention
Structure further includes liquid lens, Fig. 6 be another embodiment of the present invention provides cavity endoscope detection device structural schematic diagram, such as scheme
Shown in 6, the liquid lens 1820 is between the collimation lens 1821 and the micro electromechanical scanning galvanometer 1822, to be formed
The first new optical path, the first new optical path successively includes collimation lens 1821, the liquid lens 1820, described micro electronmechanical
Scanning galvanometer 1822, the lens 1823, the dichroscope 1824 and the object lens 1826.That is liquid lens 1820 is set
Set so that, can by liquid lens 1820 apply voltage or current make 1820 surface of liquid lens generate accordingly arrive it is curved
Song, and then the directional light that collimation lens 1821 are emitted generates different focal powers.Specific optical path are as follows: laser signal goes out from optical fiber
It penetrates, is incident on liquid lens 1820 in parallel after collimation lens 1821, from liquid lens 1820 according to the voltage or electricity of load
Flow signal and generate corresponding focal power, outgoing be converging or diverging with light by micro electromechanical scanning galvanometer 1822, lens 1823, two to
Look mirror 1824 converges on sample after relay lens 1825 is transmitted to object lens 1826.Wherein, the light that liquid lens 1820 introduces
Focal power variation can be such that the focus of the laser signal of 1826 mouthfuls of object lens outgoing is moved forward and backward in longitudinal direction, and liquid lens 1820
Response speed it is very fast, the scanning imagery of quick longitudinal direction may be implemented in KHz magnitude in scan frequency.Its
In, liquid lens 1820 is equivalent to parallel plate glass when not applying voltage or current signal, to laser signal without focal power
And the focus after object lens 1826 will not be made to generate any offset, to realize three-dimensional imaging.When specifically used, the liquid
Body lens 1820 are complementary with zoom motor 183,1826 position of object lens adjusted by zoom motor 183, in coarse adjustment to corresponding depth
Behind position, system is switched to 1820 zoom scan mode of liquid lens, carries out quick three-dimensional imaging to sample, wherein when variable
Burnt cavity endoscope detection device can also carry out when not installing zoom motor 183 only by liquid lens 1820
Zoom adjustment.
On the basis of the various embodiments described above, three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention
In the outer fixed shell include handle housing and detection pipes, as shown in figure 5, the handle housing 1811 and the detection
Pipe 1812 is fixedly connected, and the zoom motor is set to 1811 inside of handle housing, is provided in the detection pipes 1812
Detection channels, in which:
The collimation lens 1821, the micro electromechanical scanning galvanometer 1822, the lens and institute in the light channel structure
It states dichroscope to be respectively positioned in the handle housing 1811, the relay lens and the object lens in the light channel structure are respectively positioned on
In the detection channels, the object lens are located at the passway of the detection channels；
It is additionally provided with several illumination channels 1022 in the detection pipes 1812, is provided with and is used in the illumination channel 1022
The illumination fiber optic bundle of transmission illumination optical signal, wherein the illumination channel 1022 is uniform centered on the axle center of the detection channels
Distribution；
Observation channel is additionally provided in the detection pipes 1812, the observation channel is located at the detection channels and the photograph
Between bright channel 1022, observation camera lens is provided at the passway in the observation channel, the observation camera lens and the observation are logical
Light field fiber optic bundle in road is connected, to obtain the test serum regional image information before the object lens.I.e. the present invention is implemented
Several illumination channels 1022 are additionally provided in the detection pipes 1812 in cavity endoscope detection device that example provides, illuminate channel
The illumination fiber optic bundle for being used for transmission illumination optical signal is provided in 1022, wherein illumination channel 1022 is with the axle center of detection channels
Center is uniformly distributed.If being additionally provided in the detection pipes 1812 in cavity endoscope detection device i.e. provided in an embodiment of the present invention
Dry illumination channel 1022,1022 more than one of illumination channel are both provided with illumination fiber optic bundle, lighting fiber tool in each channel
There is certain pore size angle, does not need lens and be used directly for divergent illumination, and illuminate channel 1022 with the axle center of detection channels and be
Center is uniformly distributed, and Uniform Illumination is provided for cavity endoscope detection device, to facilitate the test serum before work viewing objective
Zone state.
Observation channel, the sight are additionally provided in detection pipes in cavity endoscope detection device provided in an embodiment of the present invention
It surveys channel to be located between detection channels and illumination channel, and is provided with observation camera lens 1021 and light field fiber optic bundle, light field fiber optic bundle
As imaging optical fiber bundle, for transmitting the test serum regional image information before observing the object lens that camera lens captures, wherein observing
Channel can be one, or two formation binocular observations realize three-dimensional light field cavity endoscope function, shown in Fig. 4, i.e.,
For binocular observation, Fig. 7 is the three dimensional non-linear laser scanning cavity endoscope structure schematic diagram that yet another embodiment of the invention provides,
As shown in fig. 7, imaging Multiplexing module 13 is one, corresponding observation camera lens 1021 is also one, and as monocular is observed.
On the basis of the various embodiments described above, in varifocal cavity endoscope detection device provided in an embodiment of the present invention
Be additionally provided with sorption channel in the detection pipes, as shown in Figure 1, the sorption channel 1023 be located at the illumination channel with it is described
It detects between tube edges.It is also set up in detection pipes in varifocal cavity endoscope detection device i.e. provided in an embodiment of the present invention
Have for making varifocal cavity endoscope detection device be adsorbed on the sorption channel 1023 in test serum, it is logical by extracting absorption
Air in road 1023 forms negative pressure in sorption channel 1023 so that varifocal cavity endoscope detection device be adsorbed on to
Survey tissue, wherein sorption channel 1023 is located between illumination channel and detection tube edges, that is, is located on the outside of illumination channel, leans on
At the position of nearly detection pipes avris.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror further includes air extractor, as shown in figure 3, air extractor 17 includes mainly aspiration pump, passes through exhaust pipe and sorption channel phase
Even, extraction valve is set in exhaust pipe, extraction valve is electrically connected with air extractor 17, and air extractor 17 is opened by adjusting extraction valve
The size closed and be opened and closed, controls the extraction flow of exhaust pipe, to realize that the pumping to sorption channel controls, and then adjusts
Negative pressure in sorption channel, so that zoom-type cavity endoscope detection device is adsorbed on human abdominal cavity by the effect of atmospheric pressure
The tissues such as interior stomach and intestine, oral cavity and uterine cavity reduce bio-tissue activity bring motion artifacts, so that imaging is more steady
It is fixed, clear.
Wherein, Fig. 8 is the box combination for the three dimensional non-linear laser scanning cavity endoscope that one embodiment of the invention provides
The joint sealing structural schematic diagram of structure, as shown in figure 8, it is integrated to be integrated in display 55 and the cabinet for being equipped with modules on case lid
Together, facilitate whole equipment mobile, and replacement workplace, and the display 55 is when in use, case can be placed in addition
On body, to facilitate staff to obtain the information on display, wherein in the three dimensional non-linear laser scanning cavity endoscope
Varifocal cavity endoscope detection device 1 is mouth mirror detection device.When having used in the three dimensional non-linear laser scanning cavity
After sight glass, staff can portable equipment case, convenient changing workplace uses especially in hospital, laboratory or outdoor location
The equipment can be more convenient.
Wherein, Fig. 9 be another embodiment of the present invention provides box group of three dimensional non-linear laser scanning cavity endoscope
The joint sealing structural schematic diagram of structure is closed, as shown in figure 9, being integrated in display 55 and the cabinet collection for being equipped with modules on case lid
At, facilitating whole equipment mobile together, and replacement workplace, and the display 55 is when in use, can other than be placed on
On cabinet, to facilitate staff to obtain the information on display, wherein in the three dimensional non-linear laser scanning cavity endoscope
Varifocal cavity endoscope detection device 1 be laparoscope detection device, and laparoscope detection device can be arranged simultaneously it is multiple.
After having used the three dimensional non-linear laser scanning cavity endoscope, staff can portable equipment case, convenient changing yard
Institute can be more convenient using the equipment especially in hospital, laboratory or outdoor location.
On the basis of the various embodiments described above, three dimensional non-linear laser scanning cavity endoscope provided in an embodiment of the present invention
In varifocal cavity endoscope detection device be it is multiple.Phosphor collection device i.e. provided in an embodiment of the present invention and fiber coupling
Module can be connect with multiple varifocal cavity endoscope detection device fiber optic communications simultaneously, i.e., sweep in a three dimensional non-linear laser
It retouches and integrates multiple detection devices in cavity endoscopic system, to be detected while realization to gastrointestinal tissue different parts, thus into
Row comparative analysis.
On the basis of the various embodiments described above, peeped in three dimensional non-linear laser scanning cavity provided in an embodiment of the present invention
Mirror further includes adjusting optical fiber, detects and fills with varifocal cavity endoscope respectively for phosphor collection device and fiber coupling module
Optical fiber between setting transmits connection, in which:
Adjust the adjustable in length of optical fiber.Three dimensional non-linear laser scanning cavity endoscope i.e. provided in an embodiment of the present invention
In phosphor collection device and fiber coupling module pass through length-adjustable adjusting optical fiber and varifocal cavity endoscope respectively and visit
Device progress optical fiber transmission connection is surveyed to carry out flexible movement detector to realize according to different experiments scene needs, avoided
Limit the limitation of fiber lengths, wherein the adjustable in length of optical fiber is adjusted, to realize various by the optical fiber for replacing different length
The application of occasion can carry out the optical fiber replacement of different length at any time as needed.
For the three dimensional non-linear laser scanning cavity endoscope that the various embodiments described above provide, the embodiment of the present invention is also provided
Another specific embodiment, Figure 10 provide three dimensional non-linear laser scanning cavity endoscope for one embodiment of the invention
Mesa structure schematic diagram, as shown in Figure 10, the three dimensional non-linear laser scanning cavity endoscope include that air extractor 52, first fills
Set 53, second device 54, display 55 and varifocal cavity endoscope detection device 1, wherein be integrated in first device 53
Scanning collection controller and industrial personal computer, industrial personal computer are electrically connected with display 55, second device 54 be integrated with femtosecond pulse laser,
Fiber coupling module and phosphor collection device, lighting module and image-forming module, fiber coupling module and phosphor collection device are equal
It transmits and connects with 51 optical fiber of absorption type microscope detection device, wherein varifocal cavity endoscope detection device 1 is mouth mirror spy
Survey device, for detecting human oral cavity tissue, with understand good pernicious, invasive depth, transfer case and the incisxal edge of tumour whether there is or not
The information such as Cancer residual, the absorption type three dimensional non-linear laser scanning microscope working principle is identical as the various embodiments described above, herein
It repeats no more.
Wherein, another embodiment of the present invention provides the mesa structures of three dimensional non-linear laser scanning cavity endoscope to show by Figure 11
It is intended to, as shown in figure 11, which also includes air extractor 52, first device 53, the
Two devices 54, display 55 and varifocal cavity endoscope detection device 1, wherein be integrated with scanning in first device 53 and adopt
Collection controller and industrial personal computer, industrial personal computer are electrically connected with display 55, and second device 54 is integrated with femtosecond pulse laser, optical fiber coupling
Mold block and phosphor collection device, lighting module and image-forming module, fiber coupling module and phosphor collection device with absorption
51 optical fiber of formula microscope detection device transmission connection, wherein varifocal cavity endoscope detection device 1 is laparoscope detection dress
It sets, which is embedded in human abdomen, detects to gastrointestinal tissue, to understand the good pernicious, infiltration of tumour deeply
Whether there is or not the information such as Cancer residual for degree, transfer case and incisxal edge, and the laparoscope furthermore based on the laparoscope detection device can also be used
In carrying out the detection of uterine cavity inner tissue to women, the cavity endoscope principle phase of imaging of tissue principle and the various embodiments described above is detected
Together.
Above-mentioned, although the foregoing specific embodiments of the present invention is described with reference to the accompanying drawings, not to invention protection scope
Limitation, those skilled in the art should understand that, based on the technical solutions of the present invention, those skilled in the art are not required to
The above is only preferred embodiment of the present application, are not intended to restrict the invention, for those skilled in the art, this
Application can have various modifications and variations.Within the spirit and principles of this application, made any modification, equivalent replacement,
Improve etc., it should be included within the scope of protection of this application.
The apparatus embodiments described above are merely exemplary, wherein unit can be as illustrated by the separation member
Or may not be and be physically separated, component shown as a unit may or may not be physical unit, i.e.,
It can be located in one place, or may be distributed over multiple network units.It can select according to the actual needs therein
Some or all of the modules achieves the purpose of the solution of this embodiment.Those of ordinary skill in the art are not paying creative labor
In the case where dynamic, it can understand and implement.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: it still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features；
And these are modified or replaceed, technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution spirit and
Range.
Claims (10)
1. a kind of multichannel phosphor collection device characterized by comprising
Binary channels phosphor collection module, illumination Multiplexing module and imaging Multiplexing module, the illumination Multiplexing module and it is described at
As Multiplexing module is connect with the binary channels phosphor collection module fiber optic communication, in which:
The illumination Multiplexing module for providing illumination optical signal for distal endoscope detection device, and is based on illumination fiber optic bundle
After collecting two-photon fluorescence signal and second harmonic signal, the two-photon fluorescence signal and the second harmonic signal are transmitted extremely
The binary channels phosphor collection module；
The imaging Multiplexing module, for the test serum region before distal endoscope detection device object lens to be imaged, and
After collecting two-photon fluorescence signal and second harmonic signal based on light field fiber optic bundle, the two-photon fluorescence signal and described is transmitted
Second harmonic signal is to the binary channels phosphor collection module；
The binary channels phosphor collection module, for collecting two-photon fluorescence letter based on the object lens in distal endoscope detection device
Number and second harmonic signal, and the two-photon of the synchronous convergence illumination Multiplexing module and the imaging Multiplexing module transmission
After fluorescence signal and the second harmonic signal, it is corresponding for converting the two-photon fluorescence signal and the second harmonic signal
Electric signal.
2. multichannel phosphor collection device according to claim 1, which is characterized in that the illumination Multiplexing module includes illumination light
Optical path is collected in road and the first multiplexing, in which:
Institute's illumination path successively include the illumination fiber optic bundle, first multiplexing dichroscope, variable filter, illuminating lens and
Lighting source；
It successively includes the illumination fiber optic bundle, the first multiplexing dichroscope, the first multiplexing collection that optical path is collected in first multiplexing
Lens and the first transmission fiber.
3. multichannel phosphor collection device according to claim 2, which is characterized in that the imaging Multiplexing module includes imaging
Optical path is collected in road and the second multiplexing, in which:
The imaging optical path successively includes the light field fiber optic bundle, the second multiplexing dichroscope, imaging len and camera；
It successively includes the light field fiber optic bundle, the second multiplexing dichroscope, the second multiplexing collection that optical path is collected in second multiplexing
Lens and the second transmission fiber.
4. multichannel phosphor collection device according to claim 3, which is characterized in that the binary channels phosphor collection module includes
Object lens are collected optical fiber, fiber optic universal interface, the first photomultiplier tube, the second photomultiplier tube and are connect positioned at the fiber optic universal
First between mouth and first photomultiplier tube collects optical path, is located at the fiber optic universal interface and second photoelectricity times
Second increased between pipe collects optical path, in which:
First transmission fiber, second transmission fiber and the object lens collect optical fiber with the fiber optic universal interface
Fiber optic communication connection；
The first collection optical path successively includes coupled lens, infrared fileter, the first dichroscope, the first optical filter and the
One collecting lens, wherein the first collection optical path is described for collecting the fluorescence signal that phosphor collection device receives
First photomultiplier tube is for converting the fluorescence signal as the first electric signal；
The second collection optical path successively includes the coupled lens, the infrared fileter, first dichroscope, second
Dichroscope, the second optical filter and the second collecting lens, wherein the second collection optical path is for collecting phosphor collection device
The second harmonic signal received, second photomultiplier tube is for converting the second harmonic signal as the second telecommunications
Number.
5. multichannel phosphor collection device according to claim 3, which is characterized in that the illumination Multiplexing module is multiple.
6. multichannel phosphor collection device according to claim 3, which is characterized in that the imaging Multiplexing module is multiple.
7. multichannel phosphor collection device according to claim 4, which is characterized in that it further include industrial personal computer, the binary channels
Phosphor collection module is connect with the industrial personal computer fiber optic communication, and the illumination Multiplexing module and the imaging Multiplexing module are and institute
Industry control mechatronics are stated, the industrial personal computer is generated for obtaining the first electric signal and the second electric signal, and based on the first electric signal
First fluorescent image and based on the second electric signal generate the second fluorescent image.
8. a kind of three dimensional non-linear laser scanning cavity endoscope characterized by comprising
Cavity endoscope detection device, scanning collection controller, femtosecond pulse laser, fiber coupling module and right are wanted
Seek the described in any item multichannel phosphor collection devices of 1-7, the multichannel phosphor collection device and the fiber coupling module with
The cavity endoscope detection device fiber optic communication connection, the multichannel phosphor collection device and cavity endoscope detection dress
It sets and equal is electrically connected with the scanning collection controller, in which:
The femtosecond pulse laser, for exporting pulsed laser signal to the fiber coupling module；
The fiber coupling module for coupling the pulsed laser signal of the femtosecond pulse laser output, and transmits
Collimation lens of the pulsed laser signal into the cavity endoscope detection device；
The cavity endoscope detection device exports the pulsed laser signal extremely after receiving the pulsed laser signal
The intracellular autofluorescence substance of life entity, and obtain the fluorescence signal generated after autofluorescence substance excitation and secondary
Harmonic signal, and the fluorescence signal and the second harmonic signal are exported to the phosphor collection device；
The scanning collection controller, for controlling the micro electromechanical scanning galvanometer in the cavity endoscope detection device to described
Pulsed laser signal be scanned and synchronous acquisition described in electric signal.
9. three dimensional non-linear laser scanning cavity endoscope according to claim 8, which is characterized in that peeped in the cavity
Mirror detection device includes outer fixed shell and interior clamping device, and the interior clamping device is set in the outer set casing body, institute
It states outer fixed case inside and is provided with zoom motor, to drive the relatively outer fixed shell of the interior clamping device to move up and down,
The light channel structure for being used to form the first optical path and the second optical path is provided in the interior clamping device, in which:
First optical path successively includes collimation lens, micro electromechanical scanning galvanometer, lens, dichroscope, relay lens and object lens,
Wherein first optical path is for conducting the received laser signal of the collimation lens from the collimation lens to the object lens；
Second optical path successively includes the object lens, the relay lens and the dichroscope, wherein second optical path
For conducting the collected optical signal of the object lens from the object lens to the dichroscope.
10. three dimensional non-linear laser scanning cavity endoscope according to claim 9, which is characterized in that the outer fixation
Shell includes handle housing and detection pipes, and the handle housing is fixedly connected with the detection pipes, the zoom motor setting
On the inside of the handle housing, detection channels are provided in the detection pipes, in which:
The collimation lens, the micro electromechanical scanning galvanometer, the lens and the dichroscope in the light channel structure is equal
In the handle housing, the relay lens and the object lens in the light channel structure are respectively positioned in the detection channels,
The object lens are located at the passway of the detection channels；
It is additionally provided with several illumination channels in the detection pipes, is provided in the illumination channel and is used for transmission illumination optical signal
Illumination fiber optic bundle, wherein the illumination channel is uniformly distributed centered on the axle center of the detection channels；
Be additionally provided with observation channel in the detection pipes, the observation channel be located at the detection channels and the illumination channel it
Between, observation camera lens, the bright field light in the observation camera lens and the observation channel are provided at the passway in the observation channel
Fine beam is connected, to obtain the test serum regional image information before the object lens.
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