CN210055989U

CN210055989U - Positioning type adsorption device, microscope detection device and laser scanning microscope

Info

Publication number: CN210055989U
Application number: CN201920180574.XU
Authority: CN
Inventors: 吴润龙; 王爱民; 吴丹磊; 江文茂; 胡炎辉; 徐阳阳; 程和平
Original assignee: Beijing Chaowei Landscape Biology Technology Co Ltd
Current assignee: Beijing Chaowei Landscape Biology Technology Co Ltd
Priority date: 2019-01-31
Filing date: 2019-01-31
Publication date: 2020-02-14
Anticipated expiration: 2029-01-31

Abstract

The embodiment of the utility model provides a locate mode adsorption equipment, microscope detection device and laser scanning microscope. The positioning type adsorption device comprises an outer shell, a base, a cover glass and a movement device, wherein the outer shell is detachably connected with the base through magnetic field force, and the cover glass is fixed at a sealing port of the sucker to form a built-in space and an external adsorption space; the movement device comprises a fixed support, a first driving block and a second driving block, the second driving block drives the first driving block and the fixed support to move along the Y-axis direction, and the first driving block drives the micro microscope probe to move along the X-axis direction. The embodiment of the utility model provides a locate mode adsorption equipment, microscope detecting device and laser scanning microscope realize that miniature microscope probe removes along XY axle direction in the horizontal plane through first drive block and second drive block, under unmovable adsorption equipment's the condition, look for the observation point, realize the accurate positioning of miniature microscope probe, easy operation, convenient to use.

Description

Positioning type adsorption device, microscope detection device and laser scanning microscope

Technical Field

The embodiment of the utility model provides a relate to laser scanning microscope technical field, especially relate to a locate mode adsorption equipment, microscope detection device and laser scanning microscope.

Background

With the continuous development of medicine and biology, people have made remarkable progress on the research on the cell morphology, the tissue structure or the gastrointestinal fiber state in animal life, and particularly, the related technology of obtaining the biological cell morphology of a living body by obtaining a fluorescence signal and a second harmonic signal through the excitation of pulse laser radiation in a near infrared region and the detection by a proper high-sensitivity receiver has made remarkable results.

The related detection device for acquiring the morphology of the biological cells based on the fluorescence signal, the second harmonic signal and the CARS (Coherent anti-Stokes Raman Scattering) signal plays an important role in the application of the technology. The existing imaging equipment for detecting human body cells or tissues is mainly a three-dimensional nonlinear laser scanning microscope, wherein the laser scanning microscope in the form of the existing laser scanning microscope mainly moves a microscope detection device through a mechanical arm, namely the detection device of the laser scanning microscope is arranged on the mechanical arm, the detection device is moved through the adjustment of the mechanical arm, and then different tissue structures of a human body are aligned and detected.

However, the detection device based on the mechanical arm in the three-dimensional nonlinear laser scanning microscope has a large volume, and the probe corresponds to a large skin area of a human body, so that in specific operation, specific point positions detected by the skin cannot be accurately positioned, the mechanical arm needs to be adjusted for many times, the detection device is moved for many times, the positioning effect is poor, displacement deviation is easily generated, and the imaging quality is affected.

SUMMERY OF THE UTILITY MODEL

To the technical problem who exists among the prior art, the embodiment of the utility model provides a locate mode adsorption equipment, microscope detection device and laser scanning microscope.

In a first aspect, an embodiment of the present invention provides a positioning type adsorption apparatus for setting up a micro microscope probe, including:

shell body, base, coverslip and drive the telecontrol equipment that miniature microscope probe removed in the horizontal plane, be provided with the sucking disc on the base, the sucking disc embedding the sucking disc that the shell body bottom was seted up is downthehole, the shell body with the base passes through magnetic field force and can dismantle the connection, wherein:

the cover glass is fixed at the sealing port of the sucker to form an internal space and an external adsorption space of the adsorption device;

the motion device set up in the built-in space, the motion device includes fixed bolster, first drive block and second drive block, first drive block with the second drive block respectively with fixed bolster fixed connection, the second drive block is fixed on the lateral wall of shell body, wherein:

the second driving block is used for driving the first driving block and the fixed support to move together in the Y-axis direction, and the first driving block is used for driving the micro microscope probe to move in the X-axis direction, wherein the micro microscope probe is aligned to the cover glass in the forward direction.

In a second aspect, an embodiment of the present invention provides an absorption microscope detecting device, including:

miniature microscope probe and the embodiment of the utility model provides a first aspect provides a locate mode adsorption equipment for setting up miniature microscope probe, miniature microscope probe is fixed on the first drive block, wherein:

miniature microscope probe includes probe housing and fixed plate, probe housing top is provided with first opening and second opening, probe housing bottom is provided with the third opening, be provided with in the third opening and be used for driving the zoom motor that objective reciprocated, the motor of zooming passes through the fixed plate can be dismantled and fix on the probe housing, wherein:

first opening with be provided with first light path in the first passageway that forms between the third opening, the second opening with be provided with the second light path in the second passageway that forms between the third opening, wherein:

the first optical path sequentially comprises a collimating lens, a micro-electromechanical scanning galvanometer, a first lens, a second lens, a dichroic mirror and the objective lens, wherein the collimating lens, the micro-electromechanical scanning galvanometer, the first lens, the second lens, the dichroic mirror and the objective lens are positioned between the first port and the third port, and the first optical path is used for conducting laser signals received by the first port from the first port to the third port;

the second optical path sequentially comprises the objective lens and the dichroic mirror between the third port and the second port, and the second optical path is used for conducting the optical signal collected by the objective lens to the second port from the third port.

In a third aspect, an embodiment of the present invention provides an adsorption type three-dimensional nonlinear laser scanning microscope, including:

fluorescence collection device, air exhaust device, scanning acquisition controller, femto second pulse laser instrument, fiber coupling module and the embodiment provides in the second aspect adsorption type microscope detecting device, fluorescence collection device with fiber coupling module all with adsorption type microscope detecting device optical fiber communication connects, fluorescence collection device with adsorption type microscope detecting device all with scanning acquisition controller electricity is connected, air exhaust device with adsorption type microscope detecting device electricity is connected, wherein:

the femtosecond pulse laser is used for outputting pulse laser signals to the optical fiber coupling module;

the optical fiber coupling module is used for coupling the pulse laser signal output by the femtosecond pulse laser and transmitting the pulse laser signal to the first port of the micro microscope probe in the adsorption microscope detection device;

the absorption microscope detection device is used for receiving the pulse laser signal, outputting the pulse laser signal to an autofluorescence substance in a living body cell, acquiring a fluorescence signal and a second harmonic signal generated after the autofluorescence substance is excited, and outputting the fluorescence signal and the second harmonic signal to the fluorescence collection device;

the fluorescence collecting device is used for receiving the fluorescence signal and the second harmonic signal and then respectively converting the fluorescence signal and the second harmonic signal into corresponding electric signals;

the scanning acquisition controller is used for controlling the micro microscope probe to scan the pulse laser signal and synchronously acquiring the electric signal;

and the air extracting device is used for extracting air from the outer adsorption space of the adsorption type microscope detection device so as to form negative pressure in the outer adsorption space.

The embodiment of the utility model provides a locate mode adsorption equipment, absorption formula microscope detection device and absorption formula three-dimensional nonlinear laser scanning microscope for setting up miniature microscope probe adopt the magnetism between shell body and the base to inhale the effect and can dismantle the connection together, sucking disc and cover glass form can make the outer absorption space that adsorption equipment adsorbs on the live body skin and be used for placing the built-in space of miniature microscope probe, wherein, the cover glass is fixed in the seal port of sucking disc in order to realize sealing contact, form above-mentioned outer absorption space through the inner space of sucking disc; the motion device is arranged in the built-in space, and the monolithic stationary is on the lateral wall of shell body, through first drive block and second drive block, realize that miniature microscope probe removes along XY axle direction in the horizontal plane, the integrated device is miniaturized, the device adsorbs the back on human skin, under the condition of unmovable adsorption equipment, look for the observation point, realize the accurate positioning of miniature microscope probe, obtain the formation of image in different regions, thereby the better imaging region that the observation wants to observe, and is easy to operate, high durability and convenient use, the accessible removes XY position simultaneously, form images a plurality of sites in proper order, obtain bigger formation of image field of vision through the image concatenation at last.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic structural view of a positioning type adsorption device for arranging a micro microscope probe according to an embodiment of the present invention;

fig. 2 is a schematic structural view of a combined positioning type adsorption device according to an embodiment of the present invention;

fig. 3 is a schematic view illustrating a positioning type adsorption apparatus provided by an embodiment of the present invention performing remote positioning by using cross laser;

FIG. 4 is a schematic view of a suction cup structure disposed on a base in a positioning type suction device according to an embodiment of the present invention;

fig. 5 is a schematic sectional structure view of a combined positioning type adsorption device according to an embodiment of the present invention;

fig. 6 is a schematic view of a cross-sectional structure of a combined positioning type adsorption device according to an embodiment of the present invention;

fig. 7 is a schematic structural diagram of a micro microscope probe according to an embodiment of the present invention;

fig. 8 is a schematic view of a first optical path structure in a probe of a micro microscope according to an embodiment of the present invention;

fig. 9 is a schematic view showing a cross-sectional structure of a combined positioning type adsorption device according to an embodiment of the present invention;

fig. 10 is a schematic structural view of an adsorption type three-dimensional nonlinear laser scanning microscope provided in an embodiment of the present invention;

fig. 11 is a schematic structural view of a fluorescence collecting device according to an embodiment of the present invention;

fig. 12 is a schematic view of an absorption type three-dimensional nonlinear laser scanning microscope provided by an embodiment of the present invention detecting human facial skin tissues;

fig. 13 is a schematic view of an absorption type three-dimensional nonlinear laser scanning microscope provided by the embodiment of the present invention detecting skin tissue of a chest of a human body;

fig. 14 is a schematic view of a plurality of detection devices of an absorption type three-dimensional nonlinear laser scanning microscope provided by an embodiment of the present invention detecting human skin tissues simultaneously;

fig. 15 is a schematic view of an absorption type three-dimensional nonlinear laser scanning microscope for detecting animal skin tissues according to an embodiment of the present invention;

fig. 16 is a schematic view of a box-type combination structure of an absorption type three-dimensional nonlinear laser scanning microscope according to an embodiment of the present invention;

fig. 17 is a schematic view of a box sealing structure of a box combination structure of an adsorption type three-dimensional nonlinear laser scanning microscope according to an embodiment of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

The existing related detection equipment for acquiring the form of biological cells based on fluorescence signals and second harmonic signals is mainly a three-dimensional nonlinear laser scanning microscope, and the form of the laser scanning microscope at present mainly comprises a laser scanning microscope for moving a microscope detection device through a mechanical arm, namely a detection device of the laser scanning microscope is arranged on the mechanical arm, the detection device is moved through the adjustment of the mechanical arm, and then different tissue structures of a human body are aligned and detected. However, the detection device based on the mechanical arm in the three-dimensional nonlinear laser scanning microscope has a large volume, and the probe corresponds to a large skin area of a human body, so that in specific operation, specific point positions detected by the skin cannot be accurately positioned, the mechanical arm needs to be adjusted for many times, the detection device is moved for many times, the positioning effect is poor, displacement deviation is easily generated, and the imaging quality is affected.

In order to carry out accurate location formation of image to the organism tissue for the convenience, acquire its cell morphological structure information, the embodiment of the utility model provides a locate mode adsorption equipment for setting up miniature microscope probe, figure 1 does the embodiment of the utility model provides a locate mode adsorption equipment structure sketch map for setting up miniature microscope probe, as shown in figure 1, this locate mode adsorption equipment includes:

shell body 11, base 13, coverslip 12 and drive the telecontrol equipment that miniature microscope probe 15 removed in the horizontal plane are provided with sucking disc 131 on the base 13, and the sucking disc 131 embedding shell body 11 bottom is seted up downtheholely, and shell body 11 can be dismantled through magnetic field force with base 13 and be connected, wherein:

the cover glass 12 is fixed on the sealing port 1311 of the suction cup 131 to form an inner space and an outer adsorption space of the adsorption type device;

the movement device is disposed in the built-in space, and the movement device includes a fixed bracket 142, a first driving block 141 and a second driving block 143, the first driving block 141 and the second driving block 143 are respectively fixedly connected with the fixed bracket 142, and the second driving block 143 is fixed on the side wall of the outer housing 11, wherein:

the second driving block 143 is used to drive the first driving block 141 and the fixing bracket 142 to move together in the Y-axis direction, and the first driving block 141 is used to drive the micro microscope probe 15 to move in the X-axis direction, in which the micro microscope probe 15 is aligned in the normal direction with respect to the cover glass 12.

Specifically, the embodiment of the utility model provides an outer shell 11 and base 13 in the locate mode adsorption equipment for setting up micro microscope probe 15 constitute for the material that can each other magnetism inhale, or built-in magnetic object that has each other magnetism inhale, make accessible magnetic field force can dismantle the connection together each other, and be provided with sucking disc 131 on the base 13, outer shell 11 bottom is provided with the sucking disc hole that is used for imbedding sucking disc 131, be equipped with the sealing 1311 with sucking disc 131 inner space intercommunication on sucking disc 131, when the cover glass 12 lid is on sealing 1311, form built-in space and can make adsorption equipment adsorb the outer space that adsorbs on the life skin in this locate mode adsorption equipment.

The moving device for driving the micro microscope probe 15 to move in the horizontal plane is arranged in the built-in space, and comprises a fixed support 142, a first driving block 141 and a second driving block 143, wherein the first driving block 141 and the second driving block 143 both comprise a sliding block and a fixed block, the first fixed block of the first driving block 141 is fixed on the fixed support 142, and the first sliding block of the first driving block 141 slides relative to the first fixed block under the driving of a first motor; the second sliding block of the second driving block 143 is fixed on the fixing bracket 142, the second fixing block of the second driving block 143 is fixed on the side wall of the outer shell 11, and the second sliding block of the second driving block 143 slides relative to the second fixing block under the driving of the second motor; the micro microscope probe 15 can be fixed on the first slider of the first driving block 141, so that the first driving block 141 can drive the micro microscope probe 15 to move along the X-axis direction, the second driving block 143 can drive the first driving block 141 and the fixing bracket 142 and the micro microscope probe 15 fixed on the first driving block 141 to move along the Y-axis direction together, so that the adsorption device can be roughly positioned on the skin tissue to be tested of the living body, and the micro microscope probe 15 can be driven to move along the X-axis and the Y-axis directions by the movement device, so that the micro microscope probe 15 can be accurately positioned in the horizontal plane, and meanwhile, the micro microscope probe 15 can be driven by the motor to move along the X-axis and the Y-axis directions, so that the imaging of different regions can be realized while the microscope adsorption device is not moved, and the imaging region desired to be observed can be better observed. Meanwhile, the displacement table can step by the same distance through program control, and imaged images are spliced, so that an imaging result of N times of visual field can be obtained.

And after the fixing support 142 and the micro microscope probe 15 are fixed, the micro microscope probe 15 is aligned to the cover glass 12 in the forward direction to output an internal signal and receive an external signal through the cover glass 12, thereby realizing the zoom of the microscope and the three-dimensional imaging. And the outer shell 11, the base 13, the cover glass 12 and the moving device in the figure 1 are combined into a whole to be a positioning type adsorption device for arranging the micro microscope probe 15. Fig. 2 is the embodiment of the utility model provides a locate mode adsorption equipment structural schematic diagram after the combination, as shown in fig. 2, miniature microscope probe 15 fixes on the adsorption equipment after the combination promptly for being provided with the locate mode adsorption equipment of miniature microscope probe 15, wherein miniature microscope probe 15 is located built-in space, fixes on the lateral wall of shell body 11, and miniature microscope probe 15's objective is forward to aim at the coverslip, and adsorption microscope probe's shell body 11 can be dismantled through magnetic field force with base 13 and be connected.

The embodiment of the utility model provides a locate mode adsorption equipment for setting up miniature microscope probe adopts the magnetic attraction effect between shell body and the base can be dismantled and link together, sucking disc and cover glass form the outer absorption space that can make adsorption equipment adsorb on the live body skin and the built-in space that is used for placing miniature microscope probe, wherein, the cover glass is fixed in the sealing port of sucking disc in order to realize sealed contact, forms above-mentioned outer absorption space through the inner space of sucking disc; the movement device is arranged in the built-in space and integrally fixed on the side wall of the shell, the micro microscope probe moves in the horizontal plane along the XY axis direction through the first driving block and the second driving block, the whole device is miniaturized, after the device is adsorbed on the skin of a human body, the observation point is searched while the adsorption device is not moved, the precise positioning of the micro microscope probe is realized, the imaging of different areas is obtained, and therefore the better observation of the imaging area to be observed is realized, the operation is simple, and the use is convenient.

On the basis of each embodiment, the embodiment of the utility model provides a locate mode adsorption equipment for setting up miniature microscope probe, as shown in fig. 1, still include:

first word laser instrument 16 and the first word laser instrument 17 of second, first word laser instrument 16 and the first word laser instrument 17 of second all set up in shell body 11 bottom, through transmitting cover glass and outwards adsorbing the interior transmission word laser in the space, wherein:

a first in-line laser 16 for emitting in-line laser coincident with the X axis, and a second in-line laser 17 for emitting in-line laser coincident with the Y axis. Promptly for the convenient location is surveyed, the embodiment of the utility model provides a 11 bottoms of shell body for setting up locate mode adsorption equipment of miniature microscope probe have still set up two word lasers, be located the X axle and the Y axle direction of sucking disc respectively, all transmit word laser in the outside adsorption space through the transmission cover glass, a word laser and the X axle coincidence of first word laser 16 transmission, a word laser and the Y axle coincidence of second word laser 17 transmission, thereby make the cross locating point of the cross laser that obtains coincide with the original point of XY axle and the objective optical axis center coincidence of miniature microscope probe, and the cross also coincides with XY axle. Fig. 3 is the embodiment of the utility model provides a locate mode adsorption equipment carries out remote location sketch map through cross laser, as shown in fig. 3, when locate mode adsorption equipment 1 that has miniature microscope probe is far away from the skin tissue of the life body that awaits measuring, the accessible transmission advances the cross laser in the outer adsorption space and carries out remote location, then passes through the telecontrol equipment again, carries out the accurate positioning of XY axle direction to more convenient, the accurate imaging information who acquires different regions.

miniature camera 18 and miniature illumination source 19, miniature camera 18 and miniature illumination source 19 all set up in shell body 11 bottom, wherein:

the miniature camera is a miniature CCD or a miniature CMOS;

the miniature illuminating light source is a miniature LED or an illuminating optical fiber bundle;

the lens of the miniature camera 18 faces the outer adsorption space, and the angle of the lens of the miniature camera 18 can be adjusted;

and the miniature illumination light source 19 is used for emitting light signals to the outside of the adsorption space. In order to make things convenient for the accurate positioning more promptly, the embodiment of the utility model provides a shell body 11 bottom for setting up miniature microscope probe 15's locate mode adsorption equipment has still set up miniature camera 18 and miniature illuminating light source 19, use distortionless wide-angle lens before the miniature camera 18, the camera lens is towards outside absorption space, see through the coverslip and can obtain great field of vision, when adsorption equipment adsorbs on skin, can observe miniature microscope probe 15's objective and the relative position of skin through miniature camera 18, rethread telecontrol equipment moves miniature microscope probe 15 to the position of required observation along XY axle direction. The lens angle of the miniature camera 18 can be adjusted up and down according to the requirement; the micro-lighting source 19 can emit light signals to the outside of the absorption space, and is used for providing illumination for the micro-camera 18 when the absorption device is absorbed on the skin, wherein the wavelength of the micro-lighting source for providing illumination is separated from the wavelength of the fluorescence imaging, for example, the wavelength of the second harmonic signal for imaging is 390nm and the wavelength of the two-photon autofluorescence signal for imaging is 450-600nm, and the wavelength of the LED lamp can be selected from 370nm, 635nm or infrared 850nm, 940 nm.

On the basis of the above embodiments, the embodiment of the present invention provides a suction cup in a positioning type suction device for setting a probe of a micro microscope, further comprising a suction port and an air extraction port, fig. 4 is a schematic view of a suction cup structure arranged on a base in the positioning type suction device provided by the embodiment of the present invention, as shown in fig. 4, the suction port 1312 is communicated with the sealing port 1311, and is used for being sucked on a living body to be measured through an external suction space;

the pumping port 1313 communicates with the outer adsorption space to pump the gas in the outer adsorption space. That is, the suction cup 131 disposed on the base 13 in the absorption microscope detecting device provided by the embodiment of the present invention further includes an absorption port 1312 and an extraction port 1313 in addition to the sealing port 1311, and the sealing port 1311 is communicated with the absorption port 1312 to form an internal space of the suction cup 131, when the cover glass is hermetically fixed on the sealing port 1311, the internal space of the suction cup 131 forms an external absorption space, and the absorption port 1312 is absorbed on the living body to be measured through the external absorption space; the air exhaust port 1313 communicates with the outer adsorption space, and the air in the outer adsorption space can be exhausted through the air exhaust port 1313, thereby forming a negative pressure in the outer adsorption space. Under the action of external pressure, the adsorption type microscope detection device is adsorbed on tissues such as skin of a to-be-detected living body.

Wherein, fig. 5 is the schematic view of the sectional structure of the positioning type adsorption device after combination, as shown in fig. 5, the suction cup is embedded into the suction cup hole of the outer shell 11, the cover glass 12 is fixed on the suction cup, the micro microscope probe 15 is fixed on the side wall of the outer shell 11 through the moving device 14, and the objective of the micro microscope probe 15 is aligned to the cover glass 12 in the forward direction. The micro microscope probe 15 is located in the built-in space 1314, the absorption type microscope detection device is absorbed on tissues such as skin of a to-be-detected living body through negative pressure formed in the outer absorption space 1315, an air suction opening 1313 of the suction cup is communicated with a through hole 1111 in the outer shell and used for placing an air suction pipeline, and the first linear laser 16 and the micro camera 18 are fixed at the bottom of the outer shell 11.

Wherein, fig. 6 is the schematic view of the sectional structure of the positioning type adsorption device after combination, as shown in fig. 6, the micro microscope probe 15 is located in the built-in space, and the objective of the micro microscope probe 15 is directed to the cover glass, and the second linear laser 17 and the micro illumination light source 19 are fixed at the bottom of the outer shell 11.

On the basis of the above embodiments, the embodiment of the present invention provides a fixing bracket for use in a positioning type absorption device for setting a micro microscope probe, including an X-axis bracket and a Y-axis bracket, the X-axis bracket and the Y-axis bracket being fixedly connected to each other in a perpendicular manner, wherein:

the X-axis support is detachably and fixedly connected with the first driving block, and the Y-axis support is detachably and fixedly connected with the second driving block. That is to say the embodiment of the utility model provides a fixed bolster for setting up in miniature microscope probe's locate mode adsorption equipment is the L type, and it includes X-axis support and Y-axis support, X-axis support and Y-axis support mutually perpendicular fixed connection, and fixed connection can be dismantled with the first mount of first drive block to X-axis support, and fixed connection can be dismantled with the second slider of second drive block to realize that miniature microscope probe can follow XY axle direction and remove under telecontrol equipment's drive.

The embodiment of the utility model provides a still provide an absorption formula microscope detection device, this absorption formula microscope detection device includes:

the micro microscope probe and the positioning type adsorption device for setting the micro microscope probe provided by the above embodiments, the micro microscope probe is fixed on the first driving block, wherein:

miniature microscope probe is including probe housing and fixed plate, and probe housing top is provided with first opening and second opening, and probe housing bottom is provided with the third opening, is provided with in the third opening to be used for driving the zoom motor that objective reciprocated, and the motor of zooming can be dismantled through the fixed plate and fix on probe housing, wherein:

be provided with first light path in the first passageway that forms between first opening and the third opening, be provided with the second light path in the second passageway that forms between second opening and the third opening, wherein:

the first light path sequentially comprises a collimating lens, a micro-electromechanical scanning galvanometer, a first lens, a second lens, a dichroic mirror and an objective lens, wherein the collimating lens, the micro-electromechanical scanning galvanometer, the first lens, the second lens, the dichroic mirror and the objective lens are positioned between the first port and the third port;

the second optical path comprises an objective lens and a dichroic mirror which are arranged between the third port and the second port in sequence, wherein the second optical path is used for conducting optical signals collected by the objective lens to the second port from the third port.

Specifically, fig. 7 is a schematic structural diagram of a micro microscope probe according to an embodiment of the present invention, as shown in fig. 7, three openings are disposed on a probe housing 151 of the micro microscope probe, two channels are formed between the three openings, two optical paths are disposed in the two channels, which are respectively a first optical path and a second optical path, a section of overlapped portion is disposed between the two channels formed between the three openings, and a dichroic mirror 1544 and an objective 1545 shared by the two optical paths are disposed in the overlapped portion of the channels, wherein the first optical path is an emission optical path, and sequentially includes a collimating lens 157 located between the first opening 154 and the third opening 156, a micro electro mechanical scanning galvanometer 1541, a first lens 1542, a second lens 1543, a dichroic mirror 1544, and an objective 1545, that is, a laser signal enters from the first opening 154, is conducted through the first optical path, and exits from the third opening 156, parallel light incident to the first through opening is emitted in a parallel light mode after passing through the two lenses; the second optical path is a collecting optical path and is used for outputting the collected optical signals to a collecting device connected with the probe of the micro microscope from the second port 155; the objective lens shared by the first optical path and the second optical path is installed in a high-precision zoom motor 153, and can move up and down under the driving of the zoom motor, wherein the zoom motor 153 is detachably fixed on the probe shell 151 through a fixing plate 152; and the laser signal that the first light path transmits is under the reflection of dichroic mirror 1544, can be incident into objective 1545 perpendicularly forward, the dichroic mirror reflects the laser signal of the first light path, transmit the optical signal that the second light path gathers, and the pitch angle of this dichroic mirror 1544 is adjustable, objective 1545 is the infinity objective, wherein the step precision of the above-mentioned zooming motor is less than 1 micron.

Wherein, fig. 8 is the first light path structure sketch map in the miniature microscope probe that the embodiment of the utility model provides, as shown in fig. 8, the utility model provides an in the miniature microscope probe second focus 73 of first lens 71 and the coincidence of the first focus of second lens 72, micro-electromechanical scanning galvanometer lie in first focus 75 of first lens 71, the back pupil face 74 of objective lie in the second focus of second lens 72 to form the conjugation between the light beam of incidenting to micro-electromechanical scanning galvanometer and the light beam of output to objective back pupil face 74. That is to say, the embodiment of the utility model provides an among the first light path structure in the miniature microscope probe for scanning incident laser signal's micro-electromechanical scanning galvanometer installs in first focus department 75 of first lens 71, and the coincidence of the second focus 73 of first lens 71 and the first focus of second lens 72 is through adjusting the motor that zooms for the back pupil face 74 of objective is located the first focusAt the second focal point of the two lenses 72, the conjugate is formed between the light beam incident on the micro-electromechanical scanning galvanometer and the light beam output to the back pupil surface 74 of the objective lens, so that D is obtained₂/D₁＝F₂/F₁Wherein D is₂Is the spot diameter, D, of the beam incident on the objective lens back pupil surface 74₁Is the spot diameter, F, of the light beam incident on the first focal plane of the first lens 71₂Is the focal length, F, of the second lens 72₁Is the focal length of the first lens 71; tan theta₂/tanθ₁＝F₁/F₂，θ₁Is the scan angle, θ, of the incident beam at the first focal plane of the first lens 71₂For the scanning angle of the incident beam at the objective lens back pupil surface 74, the incident laser beam is expanded by adjusting the first lens 71 and the second lens 72, so that the beam entering the objective lens fills the whole objective lens back pupil surface 74, to realize high-resolution imaging.

The embodiment of the utility model provides an absorption formula microscope detection device adopts the miniature microscope probe that installs objective additional in the locate mode absorption device and can adjust from top to bottom, move in the miniature microscope probe horizontal plane of movement device drive and carry out different imaging region accurate positioning, reciprocate through zooming motor drive miniature microscope probe, carry out the accurate detection of the different degree of depth to the life body cell form that awaits measuring, under the inter combination, thereby realize surveying the three-dimensional accurate positioning of the different regional different degree of depth of life body tissue, acquire the high resolution imaging of the cell structure of the different regional different degree of depth, and the method is simple and convenient to operate.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides an absorption formula microscope detection device still includes:

the liquid lens is positioned between the collimating lens and the micro-electromechanical scanning galvanometer to form a new first light path, and the new first light path sequentially comprises the collimating lens, the liquid lens, the micro-electromechanical scanning galvanometer, the first lens, the second lens, the dichroic mirror and the objective lens, wherein the collimating lens is positioned between the first port and the third port. That is to say, the embodiment of the present invention provides an multiplicable liquid lens 158 of first light path in the miniature microscope probe in the absorption type microscope detection device, fig. 9 is the embodiment of the present invention provides a schematic diagram of the section structure of the positioning type absorption device after combination, as shown in fig. 9, through increasing the liquid lens 158 on the miniature microscope probe 15 structure shown in fig. 9, the function of zooming is realized. The liquid lens 158 is located between the collimating lens 157 and the micro-electromechanical scanning galvanometer 1541, and in the primary optical path structure shown in fig. 9, the laser signal is parallel light after passing through the collimating lens 157. After the liquid lens 158 is added, a voltage or a current is applied to the liquid lens 158 to generate a corresponding curvature on the surface of the liquid lens 158, thereby generating different optical powers for the parallel light. The specific light path is as follows: laser signals are emitted from the optical fibers, parallelly enter the liquid lens 158 after passing through the collimating lens, corresponding focal power is generated from the liquid lens 158 according to loaded voltage or current signals, and emitted convergent or divergent light is converged on a sample after passing through the micro-electro-mechanical scanning galvanometer 1541, the first lens, the second lens, the dichroic mirror and the objective lens. The focal power change introduced by the liquid lens 158 can make the focal point of the laser signal emitted from the objective lens port move back and forth in the depth direction, and the liquid lens 158 has a very fast response speed, and the scanning frequency is in the order of KHz, so that fast depth direction scanning imaging can be realized. The liquid lens 158 is equivalent to a parallel flat glass when no voltage or current signal is applied, and has no optical power for the laser signal and does not cause any shift of the focus behind the objective lens. In specific use, the liquid lens 158 is complementary to a zoom motor, the position of an objective lens is adjusted through the zoom motor, after the objective lens is roughly adjusted to a corresponding depth position, the system is switched to a liquid lens 158 zoom scanning mode, and a sample is rapidly imaged in three dimensions, wherein when the adsorption type microscope detection device is not provided with the zoom motor, the zoom adjustment can be performed only through the liquid lens.

On the basis of the above embodiments, the embodiment of the present invention provides an outer casing in a positioning type absorption device for setting a micro microscope probe, including a first casing and a second casing, as shown in fig. 1, wherein:

an accommodating space is arranged in the first shell 111, the movement device is arranged in the accommodating space, and the first shell 111 and the second shell 112 are detachably and fixedly connected. That is, the embodiment of the present invention provides an outer casing in a positioning type adsorption device for setting a probe of a micro microscope, which comprises two parts, namely a first casing 111 and a second casing 112, wherein the first casing 111 has a containing space for setting a motion device, that is, a sucker hole in the above embodiment is also arranged at the bottom of the first casing 111, a sucker is embedded in the sucker hole of the first casing 111, and then a cover glass and the second casing 112 are combined to form an outer adsorption space and an inner space in the above embodiment, wherein the first casing 111 and the second casing 112 can be detached by a screw to be fixedly connected, so as to facilitate the assembly, disassembly and replacement of parts of the whole device.

The embodiment of the utility model provides a still provide an absorption formula three-dimensional nonlinear laser scanning microscope, fig. 10 does the embodiment of the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope structure schematic diagram, as shown in fig. 10, this laser scanning microscope includes:

fluorescence collection device 56, air exhaust device 52, scanning acquisition controller 531, femtosecond pulse laser 541, optical fiber coupling module 542 and the adsorption type microscope detection device 51 provided by the above embodiment, the fluorescence collection device 56 and the optical fiber coupling module 542 are both connected with the adsorption type microscope detection device 51 through optical fiber communication, the fluorescence collection device 56 and the adsorption type microscope detection device 51 are both electrically connected with the scanning acquisition controller 531, the air exhaust device 52 is electrically connected with the adsorption type microscope detection device 51, wherein:

the femtosecond pulse laser 541 is used for outputting a pulse laser signal to the optical fiber coupling module 542;

the optical fiber coupling module 542 is used for coupling the pulse laser signal output by the femtosecond pulse laser 541 and transmitting the pulse laser signal to a first port of a micro microscope probe in the adsorption type microscope detection device;

the absorption microscope detection device is used for receiving the pulse laser signal, outputting the pulse laser signal to the autofluorescence substance in the living body cell, acquiring a fluorescence signal and a second harmonic signal generated after the autofluorescence substance is excited through the objective lens, and outputting the fluorescence signal and the second harmonic signal to the fluorescence collection device 56;

a fluorescence collecting device 56 for receiving the fluorescence signal and the second harmonic signal and converting the fluorescence signal and the second harmonic signal into corresponding electrical signals;

the scanning acquisition controller 531 is used for controlling the micro microscope probe to scan the pulse laser signal and synchronously acquire an electric signal;

and the air exhausting device 52 is used for exhausting the outer adsorption space of the adsorption type microscope detection device so as to form negative pressure in the outer adsorption space.

Specifically, the embodiment of the present invention provides an absorption type three-dimensional nonlinear laser scanning microscope, which comprises a fluorescence collecting device 56, an air extractor 52, a scanning collecting controller 531, a femtosecond pulse laser 541, an optical fiber coupling module 542 and an absorption type microscope detecting device 51, so as to form a three-dimensional nonlinear laser scanning microscope capable of being absorbed on human skin or penetrating into intestines and stomach of human body for detection, wherein the femtosecond pulse laser 541 can emit pulse laser signals for exciting autofluorescence in human skin cells, generating multiphoton fluorescence signals and second harmonic signals, including using the 920nm femtosecond pulse laser 541 to excite FAD and collagen in cells, exciting 500-600nm fluorescence signals and 460nm second harmonic signals, and exciting autofluorescence such as FAD or NADH in cells through the 780nm femtosecond pulse laser 541, to generate corresponding fluorescent and second harmonic signals;

the fluorescence collecting device 56 integrates two signal collecting optical paths, namely a fluorescence signal collecting optical path and a second harmonic signal collecting optical path, to respectively collect the fluorescence signal and the second harmonic signal; the scanning acquisition controller 531 controls a scanning galvanometer in the probe of the micro microscope to scan the pulse laser signal and excite the self-luminous fluorescent substance to generate a fluorescent signal and a second harmonic signal, and acquires a first electric signal and a second electric signal which are obtained by converting the fluorescent signal and the second harmonic signal by the fluorescent collection device 56; the air extracting device 52 mainly comprises an air extracting pump connected with an air extracting pipeline, the air extracting pipeline is connected with the air extracting opening in the above embodiment, an air extracting valve is arranged in the air extracting pipeline, the air extracting valve is electrically connected with the air extracting device 52, the air extracting device 52 controls the air extracting flow of the air extracting pipeline by adjusting the opening and closing of the air extracting valve, thereby realizing the air extracting control of the external adsorption space, further adjust the negative pressure in the external adsorption space to ensure that the adsorption device is adsorbed on the tissues of the skin, the intestines and stomach and the like of the living body under the action of the atmospheric pressure, and the absorption type three-dimensional nonlinear laser scanning microscope can comprise a two-photon scanning microscope, a multi-photon scanning microscope and the like according to the classification, wherein, when the femtosecond pulse laser can be replaced by a common continuous laser, an aperture diaphragm is added, and the adsorption type three-dimensional nonlinear laser scanning microscope can be adjusted to be a confocal microscope. The resolution of the adsorption type three-dimensional nonlinear laser scanning microscope can be set to 800nm, the imaging field can be 200 micrometers by 200 micrometers, and the imaging speed can be 26 frames (256 pixels by 256) or 13 frames (512 pixels by 512 pixels).

The embodiment of the utility model provides an absorption three-dimensional nonlinear laser scanning microscope adopts fluorescence collection device, air exhaust device, scanning acquisition controller, femto second pulse laser, fiber coupling module and absorption formula microscope detection device, thereby form and can adsorb the three-dimensional nonlinear laser scanning microscope that human skin or deepen human intestines and stomach go on surveying, adjust the focus through adjusting the distance of miniature microscope probe and glass slide, realize laser scanning microscope's three-dimensional scanning, excite intracellular fluorescent substance spontaneous emission through femto second pulse laser and obtain multiphoton fluorescence signal and second harmonic signal, realize laser scanning microscope nonlinearity, collect fluorescence signal and second harmonic signal through fluorescence collection device, and convert corresponding electric signal, and then obtain corresponding fluorescence image etc. that reflects cell tissue structure through this electric signal, wherein, the adoption of absorption formula microscope detection device can avoid the organism activity to produce the vibration influence to miniature microscope probe, thereby avoid the vibration to influence the formation of image quality, and above-mentioned laser scanning microscope can realize multiple imaging mode and include XY formation of image, XZ formation of image and 3D formation of image, wherein XY formation of image is for carrying out the horizontal scanning formation of image on certain floor of certain degree of depth of cell structure, XZ formation of image is from the XZ section formation of image of certain degree of depth down on the top layer, 3D formation of image is from certain degree of depth down on the top layer, all carry out XY formation of image on every degree of depth, the reconsitution becomes 3D image, equipment operation is simple, high durability and convenient use.

On the basis of the foregoing embodiments, fig. 11 is a structural schematic diagram of the fluorescence collecting device provided by the embodiment of the present invention, as shown in fig. 11, the embodiment of the present invention provides a fluorescence collecting device including an optical fiber universal interface 881, a first photomultiplier 882, a second photomultiplier 883, a first collecting optical path between the optical fiber universal interface 881 and the first photomultiplier 882, and a second collecting optical path between the optical fiber universal interface 881 and the second photomultiplier 883, wherein:

the first collecting light path sequentially comprises a coupling collecting lens 81, an infrared filter 82, a first dichroic mirror 83, a first filter 84 and a first collecting lens 85, wherein the first collecting light path is used for collecting the fluorescent signal received by the fluorescent collecting device, and the first photomultiplier 882 is used for converting the fluorescent signal into a first electrical signal;

the second collecting light path sequentially includes a coupling collecting lens 81, an infrared filter 82, a first dichroic mirror 83, a second dichroic mirror 86, a second filter 87, and a second collecting lens 88, wherein the second collecting light path is configured to collect a second harmonic signal received by the fluorescence collecting device, and the second photomultiplier 883 is configured to convert the second harmonic signal into a second electrical signal. That is, the fluorescence collecting device provided by the embodiment of the present invention has a two-way signal collecting function, and integrates two light paths, wherein the first dichroic mirror 83 in the first collecting light path is a transmission fluorescence signal, the dichroic mirror for reflecting the second harmonic, the second dichroic mirror 86 and the first dichroic mirror 83 are the same dichroic mirror, and are used for reflecting the second harmonic, the first optical filter 84 is used for transmitting the fluorescence signal, and filtering the rest interference signals, and the second optical filter 87 is used for transmitting the corresponding second harmonic signal and filtering the rest interference signals, for example, when using 880nm femtosecond fiber laser to excite the autofluorescence substance in the human body surface skin cell, 390nm second harmonic signal and 450 and 600nm two-photon autofluorescence signal can be obtained, the wavelength passes through above 420nm, the dichroic mirror reflected by the wavelength below 420, that is, the first dichroic mirror 83 can separate the two-way fluorescence, clean second harmonic signals and fluorescence signals can be obtained by using the first filter 84 of 390 + -20 nm and the second filter 87 of 450-600nm, respectively.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope still includes the industrial computer, as shown in FIG. 10, industrial computer 532 is connected with scanning acquisition controller 531 electricity, wherein:

the industrial personal computer 532 is configured to obtain the first electrical signal and the second electrical signal acquired by the scanning acquisition controller 531, generate a first fluorescent image based on the first electrical signal, and generate a second fluorescent image based on the second electrical signal. That is to say the embodiment of the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope still includes the industrial computer 532 with scan acquisition controller 531 electricity is connected, this industrial computer 532 generates first fluorescence image and generates second fluorescence image based on the second signal of telecommunication based on first signal of telecommunication, can be used for showing cell structure and fiber structure information respectively, wherein install control software on the industrial computer, through control software, send control command to the scanner to control scan acquisition controller, acquire above-mentioned first signal of telecommunication and second signal of telecommunication.

On the basis of each embodiment, the embodiment of the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope still includes the display, as shown in fig. 10, display 55 is connected with industrial computer 532 electricity for show first fluorescence image and second fluorescence image. That is, the embodiment of the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope still includes the display 55 that is used for showing first fluorescence image and second fluorescence image, through display 55, the staff can directly acquire first fluorescence image and second fluorescence image's relevant information.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides an among the absorption formula three-dimensional nonlinear laser scanning microscope absorption formula microscope detection device be a plurality ofly. Namely the embodiment of the utility model provides a fluorescence collection device and fiber coupling module can be simultaneously with a plurality of absorption formula microscope detection device fiber communication connection, a plurality of detection device of integration in an absorption formula three-dimensional nonlinear laser scanning microscope system promptly to realize surveying the different tissue positions of life body simultaneously, thereby carry out contrastive analysis.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope still includes the regulation optic fibre for fluorescence collection device and fiber coupling module respectively with absorption formula microscope detection device between the optic fibre transmission be connected, wherein:

the length of the adjusting optical fiber can be adjusted. Namely the embodiment of the utility model provides a fluorescence collection device and optical fiber coupling module among absorption formula three-dimensional nonlinear laser scanning microscope carry out optical fiber transmission through adjustable length's regulation optic fibre and absorption formula microscope detection device respectively and are connected to the realization is according to different experiment scene needs, carry out nimble detection device that removes, avoid limited optical fiber length's restriction, wherein, the length adjustable who adjusts optic fibre, for the optic fibre through changing different length, realize the application of various occasions, can carry out the optic fibre change of different length as required at any time.

To illustrate the application scenario of the adsorption type three-dimensional nonlinear laser scanning microscope provided by the embodiment of the present invention more clearly, a legend is further used to explain, fig. 12 is a schematic diagram of the adsorption type three-dimensional nonlinear laser scanning microscope provided by the embodiment of the present invention for detecting the skin tissue of the face of a human body, as shown in fig. 12, the adsorption type microscope detection device 51 is adsorbed on the face of a human body by the air-extracting function of the air-extracting device 52, wherein, the first device 53 integrates a scanning acquisition controller and an industrial personal computer, the industrial personal computer is electrically connected with the display 55, the second device 54 integrates a femtosecond pulse laser, an optical fiber coupling module and a fluorescence collection device, the optical fiber coupling module and the fluorescence collection device are both in optical fiber transmission connection with the adsorption type microscope detection device 51, wherein, the working principle of the adsorption type three-dimensional nonlinear laser scanning microscope is the same as that of the, and will not be described in detail herein.

Wherein, fig. 13 is the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope surveys human chest skin tissue schematic diagram, as shown in 13, through air exhaust device 52's air exhaust function, adsorb absorption formula microscope detection device 51 at human chest, wherein, scan acquisition controller and industrial computer have been integrated in first device 53, the industrial computer is connected with display 55 electricity, second device 54 has integrated femto second pulse laser, optic fibre coupling module and fluorescence collection device all are connected with absorption formula microscope detection device 51 optical fiber transmission, wherein, this absorption formula three-dimensional nonlinear laser scanning microscope theory of operation is the same with above-mentioned each embodiment, here is no longer repeated.

Wherein, fig. 14 is a schematic view of the absorption type three-dimensional nonlinear laser scanning microscope detecting devices provided by the embodiment of the present invention for simultaneously detecting skin tissue of human body, as shown in fig. 14, a plurality of absorption type microscope detecting devices 51 are respectively and simultaneously absorbed on the face, chest and leg of human body by the air-extracting function of the air-extracting device 52, wherein, the first device 53 integrates a scanning acquisition controller and an industrial control computer, the industrial control computer is electrically connected with the display 55, the second device 54 integrates a femtosecond pulse laser, an optical fiber coupling module and a fluorescence collecting device, the optical fiber coupling module and the fluorescence collecting device are both in optical fiber transmission connection with the absorption type microscope detecting device 51, thereby, under the effect of the absorption type microscope detecting devices, the simultaneous detection of skin tissue structures of different parts of human body is realized, the operation is simple, the use is convenient, and because the absorption type microscope detecting device and the second device adopt optical fiber transmission connection, the length of the optical fiber can be adjusted, so that the human body to be measured can move freely. The working principle of the absorption type three-dimensional nonlinear laser scanning microscope is the same as that of the above embodiments, and the details are not repeated here. Fig. 15 is the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope surveys animal skin tissue sketch map, as shown in fig. 15, equally can be through air exhaust device 52's air exhaust function, adsorb absorption formula microscope detection device 51 on the skin tissue of life entity, wherein, scan acquisition controller and industrial computer have been integrated in first device 53, the industrial computer is connected with display 55 electricity, second device 54 has integrateed femto second pulse laser, fiber coupling module and fluorescence collection device all are connected with absorption formula microscope detection device 51 optical fiber transmission, its theory of operation is the same with above-mentioned each embodiment.

To the three-dimensional nonlinear laser scanning microscope of absorption formula that each above-mentioned embodiment provided, the utility model discloses the embodiment still provides another embodiment, fig. 16 is the utility model discloses the embodiment provides the box integrated configuration schematic diagram of three-dimensional nonlinear laser scanning microscope of absorption formula, as shown in fig. 16, this three-dimensional nonlinear laser scanning microscope's of absorption formula scanning microscope scanning acquisition controller 531, industrial computer 532, air exhaust device 52, fluorescence collection device 56 and femto second pulse laser and fiber coupling integrated module 540 together, integrated together in portable suitcase, the incasement has the industrial computer of taking the display screen, and integrated display 55 on the case lid of suitcase; the absorption type microscope detection device 51 is absorbed on the skin tissue of a human body to be detected, is in optical fiber communication connection with the optical fiber coupling and fluorescence collection device 56 in the box body, is connected with the air pump through an air extraction pipeline, and is electrically connected with the power plug, the scanning acquisition controller 531 and the industrial personal computer 532. Wherein figure 17 is the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope's box integrated configuration's joint sealing structure sketch map, as shown in figure 17, integrated display 55 is in the same place with the box integration of installing each module on the case lid, makes things convenient for whole equipment to remove to and change workplace, and this display 55 when using, can place outward on the box, in order to make things convenient for the staff to acquire the information on the display. After the adsorption type three-dimensional nonlinear laser scanning microscope is used, a worker can carry the equipment box by hand, so that the workplace can be conveniently replaced, and particularly, the equipment can be more conveniently used in hospitals, laboratories or outdoor places.

It should be further noted that, in the adsorption type three-dimensional nonlinear laser scanning microscope provided in the above embodiments, after changing the wavelength of the femtosecond pulse laser and adjusting the filtering range of each filter, the CARS signal can be collected on the tissue with partial fluorescence and non-SHG (second harmonic Generation) signal activity, so as to adjust the adsorption type micro CARS microscope, and specific adjustment parameters can be set according to specific requirements.

Although the present invention has been described with reference to the accompanying drawings, it is not intended to limit the scope of the invention, and it should be understood by those skilled in the art that the above is not required to be limited to the preferred embodiments of the present invention, but rather, the present invention is not limited to the above embodiments. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

The above-described embodiments of the apparatus are merely illustrative, and the units described as separate parts may or may not be physically separate, and the parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of the present embodiment. One of ordinary skill in the art can understand and implement it without inventive effort.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.

Claims

1. A locate mode adsorption equipment for setting up miniature microscope probe which characterized in that includes:

the cover glass is fixed at the sealing port of the sucker to form an internal space and an external adsorption space of the positioning type adsorption device;

the second driving block is used for driving the first driving block and the fixed support to move together along the Y-axis direction, and the first driving block is used for driving the micro microscope probe to move along the X-axis direction, wherein the micro microscope probe is aligned to the cover glass in the forward direction.

2. The apparatus of claim 1, further comprising:

first word laser instrument and first word laser instrument of second, first word laser instrument with first word laser instrument of second all set up in the shell body bottom, through the transmission the cover glass to transmit a word laser in the outer absorption space, wherein:

the first in-line laser is used for emitting in-line laser coincident with the X axis; and the second linear laser is used for emitting linear laser superposed with the Y axis.

3. The apparatus according to claim 1 or 2, further comprising:

miniature camera and miniature illumination source, miniature camera with miniature illumination source all set up in the shell body bottom, wherein:

the miniature camera is a miniature CCD or a miniature CMOS;

the lens of the miniature camera faces the outer adsorption space, and the angle of the lens of the miniature camera can be adjusted;

the miniature illumination light source is used for emitting light signals for providing illumination into the outer adsorption space.

4. The positioning type adsorption device for arranging the micro-microscope probe according to claim 1 or 2, wherein the suction cup further comprises an adsorption port and an air suction port, the adsorption port is communicated with the sealing port and is used for adsorbing a life body to be measured through the outer adsorption space;

the air pumping port is communicated with the outer adsorption space and used for pumping the air in the outer adsorption space.

5. The apparatus according to claim 1 or 2, wherein the fixed support comprises an X-axis support and a Y-axis support, and the X-axis support and the Y-axis support are vertically and fixedly connected to each other, wherein:

the X-axis support is detachably and fixedly connected with the first driving block, and the Y-axis support is detachably and fixedly connected with the second driving block.

6. An absorption microscope probe, comprising:

a micro microscope probe and the positioning and adsorbing device for setting a micro microscope probe according to any one of claims 1 to 5, the micro microscope probe being fixed on the first driving block, wherein:

7. The absorption microscope probe of claim 6, wherein the micro microscope probe further comprises:

the liquid lens is positioned between the collimating lens and the micro-electromechanical scanning galvanometer to form a new first light path, and the new first light path sequentially comprises the collimating lens, the liquid lens, the micro-electromechanical scanning galvanometer, the first lens, the second lens, the dichroic mirror and the objective lens, wherein the collimating lens is positioned between the first port and the third port.

8. An absorption type three-dimensional nonlinear laser scanning microscope, comprising:

a fluorescence collection device, an air extraction device, a scanning collection controller, a femtosecond pulse laser, an optical fiber coupling module, and the absorption microscope detection device of claim 6 or 7, wherein the fluorescence collection device and the optical fiber coupling module are both connected with the absorption microscope detection device in an optical fiber communication manner, the fluorescence collection device and the absorption microscope detection device are both electrically connected with the scanning collection controller, and the air extraction device is electrically connected with the absorption microscope detection device, wherein:

the absorption microscope detection device is used for receiving the pulse laser signal, outputting the pulse laser signal to an autofluorescent substance in a living body cell, acquiring a fluorescence signal and a second harmonic signal generated after the autofluorescent substance is excited through the objective lens, and outputting the fluorescence signal and the second harmonic signal to the fluorescence collection device;

9. The absorption three-dimensional nonlinear laser scanning microscope according to claim 8, wherein the fluorescence collecting device comprises a fiber-optic universal interface, a first photomultiplier, a second photomultiplier, and a first collecting optical path between the fiber-optic universal interface and the first photomultiplier and a second collecting optical path between the fiber-optic universal interface and the second photomultiplier, wherein:

the first collecting light path sequentially comprises a coupling collecting lens, an infrared filter, a first dichroic mirror, a first filter and a first collecting lens, wherein the first collecting light path is used for collecting the fluorescent signals received by the fluorescent collecting device, and the first photomultiplier is used for converting the fluorescent signals into first electric signals;

the second collecting light path sequentially comprises the coupling collecting lens, the infrared filter, the first dichroic mirror, the second filter and the second collecting lens, wherein the second collecting light path is used for collecting the second harmonic signals received by the fluorescence collecting device, and the second photomultiplier is used for converting the second harmonic signals into second electric signals.

10. The absorption three-dimensional nonlinear laser scanning microscope according to claim 8, wherein the absorption microscope detecting device is plural.

11. The absorption three-dimensional nonlinear laser scanning microscope according to claim 8, further comprising adjusting optical fibers for optical fiber transmission connection between the fluorescence collecting device and the optical fiber coupling module and the absorption microscope detecting device, respectively, wherein:

the length of the adjusting optical fiber is adjustable.