CN209847152U

CN209847152U - Adsorption type device, adsorption type microscope detection device and laser scanning microscope

Info

Publication number: CN209847152U
Application number: CN201920180242.1U
Authority: CN
Inventors: 吴润龙; 王爱民; 江文茂; 胡炎辉; 吴丹磊; 徐阳阳; 程和平
Original assignee: Beijing Chaowei Landscape Biology Technology Co Ltd
Current assignee: Beijing Chaowei Landscape Biology Technology Co Ltd
Priority date: 2019-01-31
Filing date: 2019-01-31
Publication date: 2019-12-27
Anticipated expiration: 2029-01-31

Abstract

An embodiment of the utility model provides an absorption formula device, absorption formula microscope detection device and laser scanning microscope. The suction device comprises an outer shell, a sucker, a cover glass and a movement device, wherein the sucker is embedded into a sucker hole, and the cover glass is fixed at a sucker sealing opening to form an inner closed space and an outer suction space; the fixed support of the movement device is fixed on the side wall of the shell, and the limiting block is connected with the fixed support in a sliding mode to drive the micro microscope probe to move up and down. The embodiment of the utility model provides an absorption formula device, absorption formula microscope detecting device and laser scanning microscope form through shell body, sucking disc and cover glass and make adsorption equipment adsorb the outer absorption space on skin and set up the interior enclosure space of probe, make the device adsorb back on skin, and miniature microscope probe sees through the cover glass and exports internal signal and receive external signal, and the whole device is miniaturized, avoids the life body activity to produce vibration influence, easy operation, convenient to use to the probe.

Description

Adsorption type device, adsorption type microscope detection device and laser scanning microscope

Technical Field

The embodiment of the utility model provides a relate to laser scanning microscope technical field, especially relate to an absorption formula device, absorption formula microscope detection device and laser scanning microscope.

Background

With the continuous development of medicine and biology, people have made remarkable progress on the research on the cell morphology, the tissue structure or the gastrointestinal fiber state in animal life, and particularly, the related technology of obtaining the biological cell morphology of a living body by obtaining a fluorescence signal and a second harmonic signal through the excitation of pulse laser radiation in a near infrared region and the detection by a proper high-sensitivity receiver has made remarkable results.

The related detection device for acquiring the morphology of the biological cells based on the fluorescence signal, the second harmonic signal and the CARS (Coherent anti-Stokes Raman Scattering) signal plays an important role in the application of the technology. The existing imaging equipment for detecting human body cells or tissues is mainly a three-dimensional nonlinear laser scanning microscope, wherein the laser scanning microscope is in a form of a laser scanning microscope based on a mechanical arm at present, namely, a detection device of the laser scanning microscope is installed on the mechanical arm, the detection device is moved by adjusting the mechanical arm, and then different tissue structures of a human body are detected in an aligning manner.

However, the detection device based on the mechanical arm in the three-dimensional nonlinear laser scanning microscope is easy to be affected by human body shake in specific operation due to the large volume and the large skin area corresponding to the probe, and the resolution requirement of three-dimensional nonlinear laser scanning imaging is generally high and is easily affected by vibration, so that the imaging quality is affected.

SUMMERY OF THE UTILITY MODEL

To the technical problem who exists among the prior art, the embodiment of the utility model provides an absorption formula device, absorption formula microscope detection device and laser scanning microscope.

In a first aspect, an embodiment of the present invention provides an adsorption type device for setting up a micro microscope probe, including:

shell body, sucking disc, cover glass and drive the telecontrol equipment that miniature microscope probe reciprocated, the sucking disc with the cover glass all set up in the shell body, wherein:

the sucking disc is embedded into a sucking disc hole formed in the bottom of the outer shell, the sucking disc is in interference fit with the sucking disc hole, and the cover glass is fixed on a sealing port of the sucking disc to form an inner closed space and an outer adsorption space of the adsorption device;

the telecontrol equipment set up in the enclosure space, the telecontrol equipment includes fixed bolster and stopper, wherein:

the fixed support is fixed on the side wall of the shell body, the limiting block is connected with the fixed support in a sliding mode relatively and used for driving the micro microscope probe to move up and down, and the micro microscope probe is aligned to the cover glass in the forward direction.

In a second aspect, an embodiment of the present invention provides an absorption microscope detecting device, including:

miniature microscope probe with the utility model provides an absorption formula device for setting up miniature microscope probe, wherein, miniature microscope probe is detachable to be fixed on the stopper, miniature microscope probe includes probe casing and fixed plate, probe casing top is provided with first opening and second opening, probe casing bottom is provided with the third opening, wherein:

first opening with be provided with first light path in the first passageway that forms between the third opening, the second opening with be provided with the second light path in the second passageway that forms between the third opening, wherein:

the first optical path sequentially comprises a collimating lens, a micro-electromechanical scanning galvanometer, a first lens, a second lens, a dichroic mirror and the objective lens, wherein the collimating lens, the micro-electromechanical scanning galvanometer, the first lens, the second lens, the dichroic mirror and the objective lens are positioned between the first port and the third port, and the first optical path is used for conducting laser signals received by the first port from the first port to the third port;

the second optical path sequentially comprises the objective lens and the dichroic mirror between the third port and the second port, and the second optical path is used for conducting the optical signal collected by the objective lens to the second port from the third port.

In a third aspect, an embodiment of the present invention provides an adsorption type three-dimensional nonlinear laser scanning microscope, including:

fluorescence collection device, air exhaust device, scanning acquisition controller, femto second pulse laser instrument, fiber coupling module and the embodiment provides in the second aspect adsorption type microscope detecting device, fluorescence collection device with fiber coupling module all with adsorption type microscope detecting device optical fiber communication connects, fluorescence collection device with adsorption type microscope detecting device all with scanning acquisition controller electricity is connected, air exhaust device with adsorption type microscope detecting device electricity is connected, wherein:

the femtosecond pulse laser is used for outputting pulse laser signals to the optical fiber coupling module;

the optical fiber coupling module is used for coupling the pulse laser signal output by the femtosecond pulse laser and transmitting the pulse laser signal to the micro microscope probe in the adsorption type microscope detection device;

the absorption microscope detection device is used for receiving the pulse laser signal, outputting the pulse laser signal to an autofluorescence substance in a living body cell, acquiring a fluorescence signal and a second harmonic signal generated after the autofluorescence substance is excited, and outputting the fluorescence signal and the second harmonic signal to the fluorescence collection device;

the fluorescence collecting device is used for receiving the fluorescence signal and the second harmonic signal and then respectively converting the fluorescence signal and the second harmonic signal into corresponding electric signals;

the scanning acquisition controller is used for controlling the micro microscope probe to scan the pulse laser signal and acquire the electric signal;

and the air extracting device is used for extracting air from the outer adsorption space of the adsorption type microscope detection device so as to form negative pressure in the outer adsorption space.

The embodiment of the utility model provides an absorption formula device, absorption formula microscope detection device and absorption formula three-dimensional nonlinear laser scanning microscope for setting up miniature microscope probe adopts shell body, sucking disc and cover glass to form and can make absorption device adsorb the outer absorption space on the life skin and be used for placing the interior enclosure space of miniature microscope probe, wherein, sucking disc and sucking disc hole interference are connected, make sucking disc and sucking disc hole sealing contact, the cover glass is fixed in the seal port of sucking disc in order to realize sealing contact, thereby form interior enclosure space and outer absorption space; the movement device is integrally fixed on the side wall of the outer shell, and slides up and down relatively with the fixed support through the limiting block, so that the micro microscope probe is moved up and down, and the micro microscope probe is positively aligned with the cover glass to output an internal signal and receive an external signal through the cover glass, the whole device is miniaturized, and after the device is adsorbed on the skin of a human body, the micro microscope probe in the suction device can be prevented from vibrating and influencing by the movement of a living body, so that the operation is simple and the use is convenient.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic structural diagram of an adsorption apparatus for installing a micro microscope probe according to an embodiment of the present invention;

fig. 2 is a schematic diagram of a suction cup structure in an adsorption apparatus for installing a micro microscope probe 15 according to an embodiment of the present invention;

fig. 3 is a schematic structural diagram of an assembled absorption type device for installing a micro microscope probe according to an embodiment of the present invention;

fig. 4 is a schematic structural view of a probe fixing frame in an adsorption apparatus for installing a micro microscope probe according to an embodiment of the present invention;

fig. 5 is a schematic cross-sectional view of an assembled absorption device for installing a probe of a micro microscope according to an embodiment of the present invention;

fig. 6 is a schematic structural diagram of a micro microscope probe in the absorption microscope detection apparatus according to the embodiment of the present invention;

fig. 7 is a schematic structural diagram of a micro microscope probe in the absorption microscope detection apparatus according to the embodiment of the present invention;

fig. 8 is a schematic structural view of an adsorption type three-dimensional nonlinear laser scanning microscope provided in an embodiment of the present invention;

fig. 9 is a schematic structural view of the fluorescence collecting device provided in the embodiment of the present invention;

fig. 10 is a schematic view of an absorption type three-dimensional nonlinear laser scanning microscope provided by an embodiment of the present invention detecting human facial skin tissues;

fig. 11 is a schematic view of an absorption type three-dimensional nonlinear laser scanning microscope provided by the embodiment of the present invention detecting skin tissue of a chest of a human body;

fig. 12 is a schematic view of a plurality of detection devices of an absorption type three-dimensional nonlinear laser scanning microscope provided by an embodiment of the present invention detecting human skin tissues simultaneously;

fig. 13 is a schematic view of an absorption type three-dimensional nonlinear laser scanning microscope for detecting animal skin tissues according to an embodiment of the present invention;

fig. 14 is a schematic view of a box-type combination structure of an absorption type three-dimensional nonlinear laser scanning microscope according to an embodiment of the present invention;

fig. 15 is a schematic view of a box sealing structure of a box combination structure of an adsorption type three-dimensional nonlinear laser scanning microscope according to an embodiment of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

The existing related detection equipment for acquiring the form of biological cells based on fluorescence signals and second harmonic signals is mainly a three-dimensional nonlinear laser scanning microscope, and the form of the existing laser scanning microscope is mainly a laser scanning microscope based on a mechanical arm, namely, a detection device of the laser scanning microscope is installed on the mechanical arm, and the detection device is moved by adjusting the mechanical arm and then aligned to detect different tissue structures of a human body. However, the laser scanning microscope has a large volume, and the probe corresponds to a large skin area of a human body, so that in specific operation, the detection device is easily affected by shaking of the human body, and the imaging quality is affected.

In order to form images to biological cell form more stably, acquire its structural information, the embodiment of the utility model provides an absorption formula device for setting up miniature microscope probe, figure 1 does the embodiment of the utility model provides an absorption formula device schematic structure diagram for setting up miniature microscope probe that is used for providing, as shown in figure 1, this absorption formula device includes:

shell body 11, sucking disc 13, cover glass 12 and drive the telecontrol equipment 14 that miniature microscope probe 15 reciprocated, sucking disc 13 and cover glass 12 all set up in shell body 11, wherein:

the sucker 13 is embedded into a sucker hole 133 formed at the bottom of the outer shell 11, the sucker 13 is in interference fit with the sucker hole 133, and the cover glass 12 is fixed at a sealing port of the sucker 13 to form an inner closed space and an outer adsorption space of the adsorption device;

the exercise device 14 is disposed in the inner closed space, and the exercise device 14 includes a fixing bracket 141 and a limiting block 142, wherein:

the fixing bracket 141 is fixed on the side wall of the outer shell 11, and the limiting block 142 is connected with the fixing bracket 141 in a relatively sliding manner, so as to drive the micro microscope probe 15 to move up and down, wherein the micro microscope probe 15 is aligned to the cover glass 12 in the forward direction.

Specifically, the embodiment of the present invention provides an outer shell 11, a suction cup 13 and a cover glass 12 in an adsorption device for disposing a micro microscope probe 15, which form an outer adsorption space for adsorbing the adsorption device on the skin of a living body and an inner closed space for placing the micro microscope probe 15, wherein the suction cup 13 and the cover glass 12 are disposed at the bottom of the outer shell 11, the bottom of the outer shell 11 is provided with a suction cup hole 133 for placing the suction cup 13, and the suction cup 13 and the suction cup hole 133 are in interference connection, so that the suction cup 13 and the suction cup hole 133 are in sealing contact, and then the cover glass 12 is fixed at the sealing port of the suction cup 13 to realize sealing contact, thereby forming the inner closed space and the outer adsorption space; the moving device 14 is integrally fixed on the side wall of the outer shell 11, the limiting block 142 and the fixing support 141 slide up and down relatively, the micro microscope probe 15 is fixed on the limiting block 142, driven by the limiting block 142, moves up and down, and after the fixing support 141 and the micro microscope probe 15 are fixed, the micro microscope probe 15 is aligned to the cover glass 12 in the forward direction to output an internal signal and receive an external signal through the cover glass 12, so that the zooming and three-dimensional imaging of the microscope are realized.

And the outer shell 11, the cover glass 12, the suction cup 13 and the movement device 14 in the figure 1 are combined into a whole to be a suction type device for arranging the micro microscope probe 15, the micro microscope probe 15 is fixed on the combined suction type device to be the suction type device for arranging the micro microscope probe 15, wherein the micro microscope probe 15 can be fixed on the limit block 142 of the moving device 14 by gluing, or detachably fixed on the limit block 142 by bolting, namely, the moving device 14 and the micro microscope probe 15 are connected through the bolt, the through hole on the stopper 141 and the first bolt hole 151 on the micro microscope probe 15, and another fixing device can be added between the stopper 141 and the micro microscope probe 15 to connect the moving device 14 and the micro microscope probe 15 through the through hole on the stopper and the second bolt hole 152 on the micro microscope probe 15.

The embodiment of the utility model provides an absorption formula device for setting up miniature microscope probe adopts shell body, sucking disc and coverslip formation can make the absorption device adsorb the outer absorption space on the life skin and be used for placing the interior enclosure space of miniature microscope probe, wherein, sucking disc and sucking disc hole interference are connected for sucking disc and sucking disc hole sealing contact, the coverslip is fixed in the seal port of sucking disc in order to realize sealing contact, thereby form interior enclosure space and outer absorption space; the motion device monolithic stationary is on the lateral wall of shell body, slides from top to bottom through stopper and fixed bolster relatively, realizes that miniature microscope probe reciprocates, and miniature microscope probe forward aims at the cover glass to see through the cover glass and export internal signal and receive external signal, the integrated device is miniaturized, and the back is adsorbed on human skin to the device, can avoid the organism activity to produce the vibration influence to miniature microscope probe among the suction attachment, easy operation, convenient to use.

On the basis of each embodiment, the embodiment of the utility model provides a sucking disc for setting up among the absorption formula device of miniature microscope probe still includes the absorption mouth, and fig. 2 does the embodiment of the utility model provides a sucking disc structure sketch map for setting up among the absorption formula device of miniature microscope probe 15, as shown in fig. 2, absorption mouth 132 is linked together with sealing port 131, wherein:

the outer side wall forming the suction port 132 is in interference connection with the suction cup hole. That is, the embodiment of the utility model provides a sucking disc 13 in the absorption formula device is still including adsorbing mouthful 132 except that sealing port 131, and sealing port 131 and adsorbing mouthful 132 are linked together, adsorbs mouthful 132 lateral wall and sucking disc hole interference connection for the sucking disc closely contacts with the shell body, thereby reunion cover glass forms seal structure.

On the basis of each embodiment, the embodiment of the utility model provides a telecontrol equipment for setting up among the absorption formula device of miniature microscope probe still includes the probe mount, and probe mount and the detachable fixed connection of stopper, miniature microscope probe pass through the probe mount detachable to be fixed on the stopper. Namely, the movement device in the adsorption device for arranging the micro microscope probe provided by the embodiment of the utility model also comprises a probe fixing frame, wherein the probe fixing frame can be fixed on the limiting block in a screw connection mode, the micro microscope probe is fixed on the limiting block through the probe fixing frame, wherein, fig. 3 is a schematic structural view of the combined absorption device for installing the micro microscope probe according to the embodiment of the present invention, as shown in fig. 3, the suction cup 13 is inserted into the suction cup hole of the outer housing 11, the cover glass 12 is fixed on the suction cup 13, the micro microscope probe 15 is fixed on the stopper by the probe fixing frame 16, namely, the through hole on the limiting block and the second bolt hole 152 shown in fig. 1 are used to fix the micro microscope probe 15 on one side of the probe fixing frame 16 and the limiting block on the other side, so that the micro microscope probe 15 is aligned to the cover glass 12 in the forward direction. Wherein, fig. 4 is the embodiment of the utility model provides a probe mount structural sketch map for setting up in the absorption formula device of miniature microscope probe, as shown in fig. 4, this probe mount is provided with stopper through-hole 161 and probe through-hole 162, and stopper through-hole 161 is used for connecting fixed stopper, and probe through-hole 162 is used for connecting miniature microscope probe.

Wherein, fig. 5 is the utility model provides a section structure sketch map after the absorption formula device combination for setting up miniature microscope probe, as shown in fig. 5, sucking disc 13 imbeds the sucking disc hole of shell body 11, cover glass 12 is fixed on sucking disc 13, miniature microscope probe 15 is fixed on the stopper, and miniature microscope probe 15 is forward to aim at cover glass 12, and miniature microscope probe is located interior enclosure 17, the absorption formula device passes through the negative pressure that forms in the outer adsorption space 18, adsorb on the tissue such as skin of the life body that awaits measuring.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides an adsorption type device for setting up miniature microscope probe still includes the motor, and the motor sets up in interior enclosure space, wherein:

the motor is rotationally connected with the limiting block through the lead screw and is used for driving the limiting block to move up and down. Namely the embodiment of the utility model provides an absorption formula device for setting up miniature microscope probe still is provided with the motor, and this motor is used for the stopper that the drive was installed miniature microscope probe to reciprocate to adjust miniature microscope probe's external focal length, thereby realize the three-dimensional detection of the different degree of depth, different levels.

On the basis of each embodiment, the embodiment of the present invention provides an outer casing in an adsorption apparatus for setting a probe of a micro microscope, including a first casing and a second casing, as shown in fig. 1, wherein:

an accommodating space is arranged in the first shell 111, the sucker and the cover glass are both arranged in the accommodating space, and the first shell 111 and the second shell 112 are detachably and fixedly connected. That is, the embodiment of the present invention provides an outer casing in an adsorption apparatus for setting up a probe of a micro microscope, which comprises two parts, namely a first casing 111 and a second casing 112, wherein the first casing 111 has a holding space for setting up a suction cup and a cover glass, that is, the suction cup hole in the above embodiment is also arranged at the bottom of the first casing 111, the suction cup is embedded in the suction cup hole of the first casing 111, and then the cover glass and the second casing 112 are combined, so as to form an outer suction space and an inner closed space in the above embodiment, wherein the first casing 111 and the second casing 112 can be detached by a screw to be fixedly connected, so as to facilitate the assembly, disassembly and replacement of parts of the whole apparatus.

The embodiment of the utility model provides a still provide an absorption formula microscope detection device, this absorption formula microscope detection device include that miniature microscope probe and above-mentioned each embodiment provide be used for setting up the absorption formula device of miniature microscope probe, wherein, miniature microscope probe is detachable fixes on the stopper, and miniature microscope probe includes probe casing and fixed plate, and probe casing top is provided with first opening and second opening, and probe casing bottom is provided with the third opening, wherein:

be provided with first light path in the first passageway that forms between first opening and the third opening, be provided with the second light path in the second passageway that forms between second opening and the third opening, wherein:

the first light path sequentially comprises a collimating lens, a micro-electromechanical scanning galvanometer, a lens, a dichroic mirror and an objective lens which are positioned between the first through hole and the third through hole, wherein the first light path is used for conducting laser signals received by the first through hole from the first through hole to the third through hole;

the second optical path comprises an objective lens and a dichroic mirror which are arranged between the third port and the second port in sequence, wherein the second optical path is used for conducting optical signals collected by the objective lens to the second port from the third port.

Specifically, fig. 6 is a schematic structural diagram of a micro microscope probe in an absorption microscope detection device according to an embodiment of the present invention, as shown in fig. 6, a probe housing 150 of the micro microscope probe is provided with three openings, two channels are formed between the three openings, two optical paths are disposed in the two channels, which are respectively a first optical path and a second optical path, a section of overlapped portion is disposed between the two channels formed between the three openings, and a dichroic mirror 1544 and an objective 1545 shared by the two optical paths are disposed in the overlapped portion of the channels, wherein the first optical path is an emission optical path, which sequentially includes a collimating lens 157, a micro electromechanical scanning mirror 1, a micro electromechanical lens 1542, a dichroic mirror 1543, and an objective 1544, which are disposed between a first opening 154 and a third opening 156, the objective is fixed on the probe housing 150 through a fixing frame 159, that is to allow laser signals to enter from the first opening 154, the parallel light is transmitted through the first optical path, is emitted from the third port 156, and is incident to the first port, passes through the two lenses, and is emitted as parallel light; the second optical path is a collecting optical path and is used for outputting the collected optical signals to a collecting device connected with the probe of the micro microscope from the second port 155; and the laser signal transmitted by the first optical path may be incident into the objective 1544 in a vertical forward direction under the reflection of the dichroic mirror 1543, the dichroic mirror reflects the laser signal of the first optical path, transmits the optical signal collected by the second optical path, and the pitch angle of the dichroic mirror 1543 is adjustable.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides an absorption formula microscope detection device still includes:

the liquid lens is located between the collimating lens and the micro-electromechanical scanning galvanometer to form a new first light path, and the new first light path sequentially comprises the collimating lens, the liquid lens, the micro-electromechanical scanning galvanometer, a lens, a dichroic mirror and an objective lens which are located between the first through hole and the third through hole. That is to say, the embodiment of the utility model provides an among the adsorption type microscope detecting device in the multiplicable liquid lens 158 of first light path among the miniature microscope probe, figure 7 is the utility model provides an among the adsorption type microscope detecting device structure schematic diagram two of miniature microscope probe, as shown in figure 7, through increase liquid lens 158 on the miniature microscope probe structure shown in figure 7, realize the function of zooming. The liquid lens 158 is located between the collimating lens 157 and the micro-electromechanical scanning galvanometer 1541, and in the primary optical path structure shown in fig. 7, the laser signal is parallel light after passing through the collimating lens 157. After the liquid lens 158 is added, a voltage or a current is applied to the liquid lens 158 to generate a corresponding curvature on the surface of the liquid lens 158, thereby generating different optical powers for the parallel light. The specific light path is as follows: laser signals are emitted from the optical fibers, parallelly enter the liquid lens 158 after passing through the collimating lens 157, corresponding focal power is generated from the liquid lens 158 according to loaded voltage or current signals, and emitted convergent or divergent light is converged on a sample after passing through the micro-electro-mechanical scanning galvanometer 1541, the lens 1542, the dichroic mirror 1543 and the objective lens 1544. The focal power change introduced by the liquid lens 158 can make the focal point of the laser signal emitted from the port of the objective lens 1544 move back and forth in the depth direction, and the liquid lens 158 has a very fast response speed, and the scanning frequency is in the KHz order, so that fast depth direction scanning imaging can be realized. The liquid lens 158 is equivalent to a parallel flat glass when no voltage or current signal is applied, and has no optical power for the laser signal and does not cause any shift of the focus behind the objective lens. In particular use, the liquid lens 158 is complementary to the motion device, the position of the objective 1544 is adjusted by the motion device, and after coarse adjustment to the corresponding depth position, the system switches to a zoom scanning mode of the liquid lens 158 for rapid three-dimensional imaging of the specimen, wherein the zoom adjustment is also possible only through the liquid lens when the suction microscopy probe is not mounted on the motion device.

The embodiment of the utility model provides a still provide an absorption formula three-dimensional nonlinear laser scanning microscope, figure 8 is the embodiment of the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope structure schematic diagram, as shown in figure 8, this laser scanning microscope includes:

fluorescence collection device 56, air exhaust device 52, scanning acquisition controller 531, femtosecond pulse laser 541, optical fiber coupling module 542 and the adsorption type microscope detection device 51 provided by the above embodiment, the fluorescence collection device 56 and the optical fiber coupling module 542 are both connected with the adsorption type microscope detection device 51 through optical fiber communication, the fluorescence collection device 56 and the adsorption type microscope detection device 51 are both electrically connected with the scanning acquisition controller 531, the air exhaust device 52 is electrically connected with the adsorption type microscope detection device 51, wherein:

the femtosecond pulse laser 541 is used for outputting a pulse laser signal to the optical fiber coupling module 542;

the optical fiber coupling module 542 is used for coupling the pulse laser signal output by the femtosecond pulse laser 541 and transmitting the pulse laser signal to a micro microscope probe in the adsorption type microscope detection device;

the absorption microscope detection device is used for receiving the pulse laser signal, outputting the pulse laser signal to the autofluorescence substance in the living body cell, acquiring a fluorescence signal and a second harmonic signal generated after the autofluorescence substance is excited, and outputting the fluorescence signal and the second harmonic signal to the fluorescence collection device 56;

a fluorescence collecting device 56 for receiving the fluorescence signal and the second harmonic signal and converting the fluorescence signal and the second harmonic signal into corresponding electrical signals;

the scanning acquisition controller 531 is used for controlling the micro microscope probe to scan the pulse laser signal and synchronously acquire an electric signal;

and the air exhausting device 52 is used for exhausting the outer adsorption space of the adsorption type microscope detection device so as to form negative pressure in the outer adsorption space.

Specifically, the embodiment of the present invention provides an absorption type three-dimensional nonlinear laser scanning microscope, which comprises a fluorescence collecting device 56, an air extractor 52, a scanning collecting controller 531, a femtosecond pulse laser 541, an optical fiber coupling module 542 and an absorption type microscope detecting device 51, so as to form a three-dimensional nonlinear laser scanning microscope capable of being absorbed on human skin or penetrating into intestines and stomach of human body for detection, wherein the femtosecond pulse laser 541 can emit pulse laser signals for exciting autofluorescence in human skin cells, generating multiphoton fluorescence signals and second harmonic signals, including using the 920nm femtosecond pulse laser 541 to excite FAD and collagen in cells, exciting 500-600nm fluorescence signals and 460nm second harmonic signals, and exciting autofluorescence such as FAD or NADH in cells through the 780nm femtosecond pulse laser 541, to generate corresponding fluorescent and second harmonic signals;

the fluorescence collecting device 56 integrates two signal collecting optical paths, namely a fluorescence signal collecting optical path and a second harmonic signal collecting optical path, to respectively collect the fluorescence signal and the second harmonic signal; the scanning acquisition controller 531 controls a scanning galvanometer in the probe of the micro microscope to scan the pulse laser signal and excite the self-luminous fluorescent substance to generate a fluorescent signal and a second harmonic signal, and acquires a first electric signal and a second electric signal which are obtained by converting the fluorescent signal and the second harmonic signal by the fluorescent collection device 56; the air extractor 52 mainly includes an air extracting pump connected to an air extracting pipeline, an air extracting valve is set in the air extracting pipeline, the air extracting valve is electrically connected to the air extractor 52, the air extractor 52 controls the air extracting flow of the air extracting pipeline by adjusting the opening and closing of the air extracting valve, thereby realizing the air extracting control of the external adsorption space, and further adjusting the negative pressure in the external adsorption space, so that the adsorption device is adsorbed on the tissues of the skin, intestines and stomach of the living body through the effect of atmospheric pressure, and the adsorption type three-dimensional nonlinear laser scanning microscope can include a two-photon scanning microscope and a multiphoton scanning microscope according to the classification, wherein, when the femtosecond pulse laser can be replaced by a common continuous laser, a small aperture diaphragm is added, and the adsorption type three-dimensional nonlinear laser scanning microscope can also be adjusted to be a confocal microscope. The resolution of the adsorption type three-dimensional nonlinear laser scanning microscope can be set to 800nm, the imaging field can be 200 micrometers by 200 micrometers, and the imaging speed can be 26 frames (256 pixels by 256) or 13 frames (512 pixels by 512 pixels).

The embodiment of the utility model provides an absorption three-dimensional nonlinear laser scanning microscope adopts fluorescence collection device, air exhaust device, scanning acquisition controller, femto second pulse laser, fiber coupling module and absorption formula microscope detection device, thereby form and can adsorb the three-dimensional nonlinear laser scanning microscope that human skin or deepen human intestines and stomach go on surveying, adjust the focus through adjusting the distance of miniature microscope probe and glass slide, realize laser scanning microscope's three-dimensional scanning, excite intracellular fluorescent substance spontaneous emission through femto second pulse laser and obtain multiphoton fluorescence signal and second harmonic signal, realize laser scanning microscope nonlinearity, collect fluorescence signal and second harmonic signal through fluorescence collection device, and convert corresponding electric signal, and then obtain corresponding fluorescence image etc. that reflects cell tissue structure through this electric signal, wherein, the adoption of absorption formula microscope detection device can avoid the organism activity to produce the vibration influence to miniature microscope probe, thereby avoid the vibration to influence the formation of image quality, and above-mentioned laser scanning microscope can realize multiple imaging mode and include XY formation of image, XZ formation of image and 3D formation of image, wherein XY formation of image is for carrying out the horizontal scanning formation of image on certain floor of certain degree of depth of cell structure, XZ formation of image is from the XZ section formation of image of certain degree of depth down on the top layer, 3D formation of image is from certain degree of depth down on the top layer, all carry out XY formation of image on every degree of depth, the reconsitution becomes 3D image, equipment operation is simple, high durability and convenient use.

On the basis of each embodiment, fig. 9 is the utility model discloses fluorescence collection device schematic structure diagram that the embodiment provided, as shown in fig. 9, the utility model discloses the fluorescence collection device that provides includes optic fibre general interface 781, first photomultiplier 782, second photomultiplier 783 and is located the first collection light path between optic fibre general interface 781 and the first photomultiplier 782, is located the second collection light path between optic fibre general interface 781 and the second photomultiplier 783, wherein:

the first collecting light path sequentially comprises a coupling collecting lens 71, an infrared filter 72, a first dichroic mirror 73, a first filter 74 and a first collecting lens 75, wherein the first collecting light path is used for collecting the fluorescent signal received by the fluorescent collecting device, and the first photomultiplier 782 is used for converting the fluorescent signal into a first electrical signal;

the second collecting optical path sequentially includes a coupling collecting lens 71, an infrared filter 72, a first dichroic mirror 73, a second dichroic mirror 76, a second filter 77, and a second collecting lens 78, wherein the second collecting optical path is configured to collect a second harmonic signal received by the fluorescence collecting device, and the second photomultiplier 783 is configured to convert the second harmonic signal into a second electrical signal. That is, the fluorescence collecting device provided by the embodiment of the present invention has a two-way signal collecting function, and integrates two light paths, wherein the first dichroic mirror 73 in the first collecting light path is a transmission fluorescence signal, the dichroic mirror for reflecting the second harmonic, the second dichroic mirror 76 and the first dichroic mirror 73 are the same dichroic mirror for reflecting the second harmonic, the first optical filter 74 is used for transmitting the fluorescence signal and filtering the rest interference signals, and the second optical filter 77 is used for transmitting the corresponding second harmonic signal and filtering the rest interference signals, for example, when using 780nm femtosecond fiber laser to excite the autofluorescence substance in the human body surface skin cell, 390nm second harmonic signal and 450 and 600nm two-photon autofluorescence signal can be obtained, the wavelength passes through above 420nm, the dichroic mirror reflected by the wavelength below 420, that is, the first dichroic mirror 73 can separate the two-way fluorescence, clean second harmonic signals and fluorescence signals can be obtained by using the first filter 74 of 390 + -20 nm and the second filter 77 of 450-600nm, respectively.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope still includes the industrial computer, as shown in FIG. 8, industrial computer 532 is connected with scanning acquisition controller 531 electricity, wherein:

the industrial personal computer 532 is configured to obtain the first electrical signal and the second electrical signal acquired by the scanning acquisition controller 531, generate a first fluorescent image based on the first electrical signal, and generate a second fluorescent image based on the second electrical signal. That is to say the embodiment of the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope still includes the industrial computer 532 with scan acquisition controller 531 electricity is connected, this industrial computer 532 generates first fluorescence image and generates second fluorescence image based on the second signal of telecommunication based on first signal of telecommunication, can be used for showing cell structure and fiber structure information respectively, wherein install control software on the industrial computer, through control software, send control command to the scanner to control scan acquisition controller, acquire above-mentioned first signal of telecommunication and second signal of telecommunication.

On the basis of each embodiment, the embodiment of the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope still includes the display, as shown in FIG. 8, display 55 is connected with industrial computer 532 electricity for show first fluorescence image and second fluorescence image. That is, the embodiment of the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope still includes the display 55 that is used for showing first fluorescence image and second fluorescence image, through display 55, the staff can directly acquire first fluorescence image and second fluorescence image's relevant information.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides an among the absorption formula three-dimensional nonlinear laser scanning microscope absorption formula microscope detection device be a plurality ofly. Namely the embodiment of the utility model provides a fluorescence collection device and fiber coupling module can be simultaneously with a plurality of absorption formula microscope detection device fiber communication connection, a plurality of detection device of integration in an absorption formula three-dimensional nonlinear laser scanning microscope system promptly to realize surveying the different tissue positions of life body simultaneously, thereby carry out contrastive analysis.

On the basis of above-mentioned each embodiment, the embodiment of the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope still includes the regulation optic fibre for fluorescence collection device and fiber coupling module respectively with absorption formula microscope detection device between the optic fibre transmission be connected, wherein:

the length of the adjusting optical fiber can be adjusted. Namely the embodiment of the utility model provides a fluorescence collection device and optical fiber coupling module among absorption formula three-dimensional nonlinear laser scanning microscope carry out optical fiber transmission through adjustable length's regulation optic fibre and absorption formula microscope detection device respectively and are connected to the realization is according to different experiment scene needs, carry out nimble detection device that removes, avoid limited optical fiber length's restriction, wherein, the length adjustable who adjusts optic fibre, for the optic fibre through changing different length, realize the application of various occasions, can carry out the optic fibre change of different length as required at any time.

To illustrate the application scenario of the adsorption type three-dimensional nonlinear laser scanning microscope provided by the embodiment of the present invention more clearly, a legend is further used to explain, fig. 10 is a schematic diagram of the adsorption type three-dimensional nonlinear laser scanning microscope provided by the embodiment of the present invention for detecting the skin tissue of the face of a human body, as shown in fig. 10, the adsorption type microscope detection device 51 is adsorbed on the face of a human body by the air-extracting function of the air-extracting device 52, wherein, the first device 53 integrates a scanning acquisition controller and an industrial personal computer, the industrial personal computer is electrically connected with the display 55, the second device 54 integrates a femtosecond pulse laser, an optical fiber coupling module and a fluorescence collection device, the optical fiber coupling module and the fluorescence collection device are both in optical fiber transmission connection with the adsorption type microscope detection device 51, wherein, the working principle of the adsorption type three-dimensional nonlinear laser scanning microscope is the same as that of the, and will not be described in detail herein.

Wherein, fig. 11 is the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope surveys human chest skin tissue schematic diagram, as 11 shows, the function of bleeding through air exhaust device 52, adsorb absorption formula microscope detection device 51 at human chest, wherein, scan acquisition controller and industrial computer have been integrated in first device 53, the industrial computer is connected with display 55 electricity, second device 54 has integrated femto second pulse laser, optic fibre coupling module and fluorescence collection device all are connected with absorption formula microscope detection device 51 optical fiber transmission, wherein, this absorption formula three-dimensional nonlinear laser scanning microscope theory of operation is the same with above-mentioned each embodiment, here is no longer repeated.

Wherein, fig. 12 is a schematic view of the absorption type three-dimensional nonlinear laser scanning microscope multiple detection devices provided by the embodiment of the present invention for simultaneously detecting skin tissue of human body, as shown in fig. 12, the multiple absorption type microscope detection devices 51 are respectively and simultaneously absorbed on the face, chest and leg of human body by the air-extracting function of the air-extracting device 52, wherein, the first device 53 integrates a scanning acquisition controller and an industrial control computer, the industrial control computer is electrically connected with the display 55, the second device 54 integrates a femtosecond pulse laser, an optical fiber coupling module and a fluorescence collecting device, the optical fiber coupling module and the fluorescence collecting device are both in optical fiber transmission connection with the absorption type microscope detection device 51, thereby, under the effect of the multiple absorption type microscope detection devices, the simultaneous detection of skin tissue structures of different parts of human body is realized, the operation is simple, the use is convenient, and because the absorption type microscope detection device and the second device are connected by optical fiber communication, the length of the optical fiber can be adjusted, so that the human body to be measured can move freely. The working principle of the absorption type three-dimensional nonlinear laser scanning microscope is the same as that of the above embodiments, and the details are not repeated here. Fig. 13 is the embodiment of the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope surveys animal skin tissue sketch map, as shown in fig. 13, equally can be through air exhaust device 52's air exhaust function, adsorb absorption formula microscope detection device 51 on the skin tissue of life entity, wherein, scan acquisition controller and industrial computer have been integrated in first device 53, the industrial computer is connected with display 55 electricity, second device 54 has integrated femto second pulse laser, fiber coupling module and fluorescence collection device all are connected with absorption formula microscope detection device 51 optical fiber transmission, its theory of operation is the same with above-mentioned each embodiment.

To the three-dimensional nonlinear laser scanning microscope of absorption formula that each above-mentioned embodiment provided, the utility model discloses the embodiment still provides another embodiment, fig. 14 is the utility model discloses the embodiment provides box integrated configuration schematic diagram of three-dimensional nonlinear laser scanning microscope of absorption formula, as shown in fig. 14, this three-dimensional nonlinear laser scanning microscope's of absorption formula scanning microscope scanning acquisition controller 531, industrial computer 532, air exhaust device 52, fluorescence collection device 56 and femto second pulse laser and fiber coupling integrated module 540 together, integrated together in portable suitcase, the incasement has the industrial computer of taking the display screen, and integrated display 55 on the case lid of suitcase; the absorption type microscope detection device 51 is absorbed on the skin tissue of a human body to be detected, is in optical fiber communication connection with the optical fiber coupling and fluorescence collection device 56 in the box body, is connected with the air pump through an air extraction pipeline, and is electrically connected with the power plug, the scanning acquisition controller 531 and the industrial personal computer 532. Wherein figure 15 is the utility model provides an absorption formula three-dimensional nonlinear laser scanning microscope's box integrated configuration's joint sealing structure sketch map, as shown in figure 15, integrated display 55 is in the same place with the box integration of installing each module on the case lid, makes things convenient for whole equipment to remove to and change workplace, and this display 55 when using, can place outward on the box, in order to make things convenient for the staff to acquire the information on the display. After the adsorption type three-dimensional nonlinear laser scanning microscope is used, a worker can carry the equipment box by hand, so that the workplace can be conveniently replaced, and particularly, the equipment can be more conveniently used in hospitals, laboratories or outdoor places.

It should be further noted that, in the adsorption type three-dimensional nonlinear laser scanning microscope provided in the above embodiments, after changing the wavelength of the femtosecond pulse laser and adjusting the filtering range of each filter, the CARS signal can be collected on the tissue with partial fluorescence and non-SHG (second harmonic Generation) signal activity, so as to adjust the adsorption type micro CARS microscope, and specific adjustment parameters can be set according to specific requirements.

Although the present invention has been described with reference to the accompanying drawings, it is not intended to limit the scope of the invention, and it should be understood by those skilled in the art that the above is not required to be limited to the preferred embodiments of the present invention, but rather, the present invention is not limited to the above embodiments. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

The above-described embodiments of the apparatus are merely illustrative, and the units described as separate parts may or may not be physically separate, and the parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of the present embodiment. One of ordinary skill in the art can understand and implement it without inventive effort.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.

Claims

1. An adsorption-type device for setting up a micro-microscope probe, comprising:

2. The suction device for setting up a micro-microscope probe according to claim 1, wherein the suction cup further comprises a suction port in communication with the sealing port, wherein:

the outer side wall forming the adsorption port is in interference connection with the sucker hole.

3. The adsorption-type device for arranging a micro microscope probe according to claim 1, wherein the movement device further comprises a probe fixing frame, the probe fixing frame is detachably and fixedly connected with the limiting block, and the micro microscope probe is detachably fixed on the limiting block through the probe fixing frame.

4. The suction device for positioning a micro-microscope probe according to claim 1, further comprising a motor disposed within the inner enclosed space, wherein:

the motor is connected with the limiting block in a rotating mode through a lead screw and is used for driving the limiting block to move up and down.

5. The suction device for setting up a micro-microscope probe according to claim 1, wherein the outer housing comprises a first housing and a second housing, wherein:

the glass cover is characterized in that an accommodating space is formed in the first shell, the sucker and the cover glass are arranged in the accommodating space, and the first shell and the second shell are detachably and fixedly connected.

6. An absorption microscope probe device, comprising a micro microscope probe and the absorption microscope probe device of any one of claims 1 to 5, wherein the micro microscope probe is detachably fixed on the stopper, the micro microscope probe comprises a probe housing and a fixing plate, the probe housing is provided with a first opening and a second opening at the top end, the probe housing is provided with a third opening at the bottom end, wherein:

the first optical path sequentially comprises a collimating lens, a micro-electromechanical scanning galvanometer, a lens, a dichroic mirror and an objective lens, wherein the collimating lens, the micro-electromechanical scanning galvanometer, the lens, the dichroic mirror and the objective lens are positioned between the first port and the third port, and the first optical path is used for conducting the laser signal received by the first port from the first port to the third port;

7. The absorption microscope probe of claim 6, wherein the micro microscope probe further comprises:

the liquid lens is positioned between the collimating lens and the micro-electromechanical scanning galvanometer to form a new first light path, and the new first light path sequentially comprises the collimating lens, the liquid lens, the micro-electromechanical scanning galvanometer, the lens, the dichroic mirror and the objective lens, wherein the collimating lens, the liquid lens, the micro-electromechanical scanning galvanometer, the lens and the objective lens are positioned between the first through hole and the third through hole.

8. An absorption type three-dimensional nonlinear laser scanning microscope, comprising:

a fluorescence collection device, an air extraction device, a scanning collection controller, a femtosecond pulse laser, an optical fiber coupling module, and the absorption microscope detection device of claim 6 or 7, wherein the fluorescence collection device and the optical fiber coupling module are both connected with the absorption microscope detection device in an optical fiber communication manner, the fluorescence collection device and the absorption microscope detection device are both electrically connected with the scanning collection controller, and the air extraction device is electrically connected with the absorption microscope detection device, wherein:

the scanning acquisition controller is used for controlling the micro microscope probe to scan the pulse laser signal and synchronously acquiring the electric signal;

9. The absorption three-dimensional nonlinear laser scanning microscope according to claim 8, wherein the fluorescence collecting device comprises a fiber-optic universal interface, a first photomultiplier, a second photomultiplier, and a first collecting optical path between the fiber-optic universal interface and the first photomultiplier and a second collecting optical path between the fiber-optic universal interface and the second photomultiplier, wherein: the first collecting light path sequentially comprises a coupling collecting lens, an infrared filter, a first dichroic mirror, a first filter and a first collecting lens, wherein the first collecting light path is used for collecting the fluorescent signals received by the fluorescent collecting device, and the first photomultiplier is used for converting the fluorescent signals into first electric signals;

the second collecting light path sequentially comprises the coupling collecting lens, the infrared filter, the first dichroic mirror, the second filter and the second collecting lens, wherein the second collecting light path is used for collecting the second harmonic signals received by the fluorescence collecting device, and the second photomultiplier is used for converting the second harmonic signals into second electric signals.

10. The absorption three-dimensional nonlinear laser scanning microscope according to claim 8, wherein the absorption microscope detecting device is plural.

11. The absorption three-dimensional nonlinear laser scanning microscope according to claim 8, further comprising adjusting optical fibers for optical fiber transmission connection between the fluorescence collecting device and the optical fiber coupling module and the absorption microscope detecting device, respectively, wherein:

the length of the adjusting optical fiber is adjustable.