CN109673444A

CN109673444A - A kind of high-yield planting method of drought lotus root

Info

Publication number: CN109673444A
Application number: CN201710765120.4A
Authority: CN
Inventors: 谭美才
Original assignee: Dahua Natural Longevity Food Co Ltd
Current assignee: Dahua Natural Longevity Food Co Ltd
Priority date: 2017-08-30
Filing date: 2017-08-30
Publication date: 2019-04-26

Abstract

The present invention relates to agricultural technology fields, and in particular to a kind of high-yield planting method of drought lotus root includes the following steps, (1) field processing: including selection of land, site preparation and ridge；(2) seed treatment: non-irrigated lotus root seed of the selection without lesion of rotting, each drought lotus root seed is a section kind block, each kind of bud 2-3, block band, kind of block is carried out disinfection using thimerosal, the thimerosal include in parts by weight hundred mattress of 1-5 part clearly, 3-8 parts of salsola collina extracting solutions, 3-8 parts of swainson pea extracting solutions, 3-8 parts of castor-oil plant extracting solutions, 20-50 parts of distilled water；(3) it sows；(4) field management: including weeding, fertilising, ridging and the prevention and control of plant diseases, pest control.The invention has the advantages that this method is simple and easy, survival rate can be improved, reduce pest and disease damage incidence, increase non-irrigated lotus root tiller number, and reduce the harmful components of waste, is sufficiently fed non-irrigated lotus root beneficial to sized molecules in release waste and is absorbed and utilized, shorten the high yield period of non-irrigated lotus root, economic value with higher.

Description

A kind of high-yield planting method of drought lotus root

[technical field]

The present invention relates to agricultural technology fields, and in particular to a kind of high-yield planting method of drought lotus root.

[background technique]

Non-irrigated lotus root, cauline leaf are grown in Subtropical hill mountainous region as taro alias banana dasheen, beauty's edible canna like canna, root tuber, It is that Cannaceae canna belongs to perennial unifacial leaf herbaceous plant.Non-irrigated lotus root originates in Malaysia, introduces China the 1950s Southern mountain areas, since its fertility is strong, easy to raise is now distributed widely in Yunnan Province of China, Guizhou, Sichuan, Chongqing, river The provinces such as south, Hunan, Guangxi.Early lotus root is full of nutrition, and fat content is very low, except starch-containing, glucose, eats that liquid eggs is white, diet is fine Dimension is outer, also contains calcium, iron, phosphorus, 17 kinds of amino acid and multivitamin, have improve a poor appetite, digestion-promoting rouge, Appetizing spleen-tonifying are made With.The bean vermicelli processed using dry lotus root starch has the characteristics that late-maturing resistance to rotten, long boil is not pasted, and smooth in taste can be used as chafing dish, fry Powder, cold and dressed with sauce, soup such as boil at the culinary art food materials.As people are continuously increased healthy food demand, the demands such as dry lotus root starch, dry lotus root vermicelli Amount will be gradually increased.

Guangxi hydrothermal resources are abundant, and loosing soil, permeability is strong, are suitable for non-irrigated lotus root growth." the Guangxi 2011-2015 kind The planning of Zhi Ye superior product Regional Distribution " it points out, early lotus root is the two of the Ways of Special Agricultural Products that Nanning City, Hechi City are given priority to, 2015 annual planting areas were doubled than 2005, and stem tuber per unit area yield improves 30%.Current drought lotus root cultivation management is extensive, cultivation technique Fall behind relatively, based on growing naturally in field, Around the house, the natural environment of growth is poor, low output.Therefore one is found A suitable planting patterns improves the yield of non-irrigated lotus root, can be only achieved to the purpose of increasing peasant income.

[summary of the invention]

Goal of the invention of the invention is: for above-mentioned problem, the present invention provides a kind of high-yield planting method of non-irrigated lotus root, This method is simple and easy, and survival rate can be improved, and reduces pest and disease damage Probability, increases the tiller number of non-irrigated lotus root bud, and can reduce useless The harmful components of gurry discharge in waste and are sufficiently fed and are absorbed and utilized to non-irrigated lotus root beneficial to sized molecules, shorten the rich of non-irrigated lotus root Term improves the quality of non-irrigated lotus root, economic value with higher.

To achieve the goals above, The technical solution adopted by the invention is as follows:

A kind of high-yield planting method of drought lotus root, specifically includes following steps,

(1) field is handled: including selection of land, site preparation and ridge；

A, selection of land: the clayed soil that selection is sunny, soil property is fertile, soil layer is deep, loose fertilizer conservation water-retaining property is strong；

B, site preparation: digging to field before passing the winter, and deep ploughs 25-35 centimetres when digging, and shines the soil wind-engaging sun climbed over Dry to pass the winter, the upturned soil tedded deep ploughing when site preparation is thin, carefully harrows hack, makes that the soil is porous；

C, ridge: a height of 30 centimetres of row, on row face wide 80 for centimetre, it is wide 40 centimetres on the intermediate groove face of row；

(2) seed treatment: non-irrigated lotus root seed of the selection without lesion of rotting, each drought lotus root seed is a section kind block, each kind of block Bud 2-3, band, kind block is carried out disinfection using thimerosal,

In parts by weight, including hundred mattress of 1-5 part is clear, 3-8 part salsola collina extracting solutions, 3-8 parts of hardship horses for the thimerosal Beans extracting solution, 3-8 parts of castor-oil plant extracting solutions, 20-50 parts of distilled water；

(3) it sows: the kind block after disinfection is sowed, 1-3 kind of blocks of each cave hole sowing, width 25-35 is cheated in each cave Centimetre, deep 20-25 centimetres, the cave hole held successfully first backfills the 3-5 cm thick that loosens the soil, and applies biological organic fertilizer 1.5-2kg later, connects On biological organic fertilizer return one layer of about 1-3 cm thick of lid loose fine earth, dissipate biological organic fertilizer 0.5-1kg again later in pine On fine earth, then the loose fine earth of one layer of 5-7 cm thick of lid is returned, then enter the kind block flicking after disinfection in fine earth, and plants block Kind block upward, is finally returned training leveling with moist soil, makes 2-3 centimetres of block of Hui Tuman lid kind, 100-120 lis of the line-spacing of sowing by bud without exception Rice, 100-110 centimetres of spacing in the rows；

The biological organic fertilizer includes that following raw material is made in parts by weight: 20-30 parts by weight peat, 20-30 weight Maize pulp, 5-10 parts by weight mulberry-leaf extract, 5-10 parts by weight rice bran after measuring part bagasse, 20-30 parts by weight fermentation alcohol Extract, 3-5 parts by weight Rhodopseudomonas palustris, 3-5 parts by weight sour soil bacillus, 3-5 parts by weight lactobacillus acidophilus；

(4) field management: including weeding, fertilising, ridging and the prevention and control of plant diseases, pest control；And the field management in two stages into Row carries out when seedling is 30-40 centimetres long after the first stage is non-irrigated lotus root kind block insemination and emergence；Second stage be current year 8-9 month or It is carried out before blooming；

S1, weeding: mode of the application herbicide in conjunction with artificial weeding carries out weeding, and when applying herbicide, herbicide is not It is direct with non-irrigated lotus root stem or blade, guarantee on the ground in field without weeds；

S2, fertilising: the first stage is cheated with the cave that every plant of non-irrigated lotus root kind block is sowed as center of radius, and radius is 5-10 centimetres and opens depth 30-50g urea then earthing is added in 5 centimetres wide 5 centimetres of ditch；Second stage is with the cave hole of every plant of non-irrigated lotus root kind block sowing For center of radius, radius is 5-10 centimetres and opens deep 5 centimetres wide 5 centimetres of ditch, applies 50-80g phosphorus potash fertilizer then earthing, then will Cornstalk straw or straw are averagely bedded in the left and right sides of non-irrigated lotus root root, later in the fine lower liquid manure with field head manufacture of weather sun It fills respectively and pours Hang Gouli, the liquid manure amount for filling leaching has seen water with ground, and the water-rich soil flip lid in ditch is arrived again every two days On cornstalk straw or straw around non-irrigated lotus root root；

S3, ridging: to promote the growth of non-irrigated lotus root and preventing the big lodging of later period wind, growth period need to earth up 1-2 times；

S4, the prevention and control of plant diseases, pest control: being directed to bud rot, stem rot, and gram bacterium star or rotten ichor for spraying 500 times of dilution are prevented It controls；The ZhiBingLing(SIC) of 1000 times of dilution is sprayed for virosis or viral ichor is prevented and treated；For rust apply the mould pulvis of star into Row prevention and treatment；1000 times of liquid are diluted for budworm application 40% dimethoate emulsifiable concentrate of mass concentration to spray；It is dense for any of several broadleaf plants insect parasite which hurts rice plant application quality 90% trichlorfon technical, 800 times of liquid of dilution are spent to spray.

It further illustrates, in step (2), in parts by weight, including 3 part of hundred mattress is clear, 5 parts of pigs hairs for the thimerosal Dish extracting solution, 5 parts of swainson pea extracting solutions, 5 parts of castor-oil plant extracting solutions, 40 parts of distilled water.

It further illustrates, the hair dish extracting solution is added 75% ethyl alcohol of mass concentration after cleaning hair dish and is heated to reflux It extracts 30 minutes, filters to take filtrate, be then concentrated into 1.01 times of original volume.

It further illustrates, the swainson pea extracting solution is with hot-water soak 5 minutes of 40 degrees Celsius by horsebean then by beans Skin removes, and the water for being subsequently added into 2 times of volumes is beaten, and 0.2 times of slurry weight of diatomite stirring is then added, is then added After the ethyl alcohol stirring that mass fraction is 75% stands 12 hours, by filtering to take filtrate.

It further illustrates, the castor-oil plant extracting solution is that mass fraction is added as the progress of 80% ethyl alcohol after crushing castor leaf It is heated to 70 degrees Celsius of refluxing extractions 3 times, 30 minutes every time, then filters to take filtrate, 2 times of (v/v) water then are added in filtrate, Then active carbon is added and is heated to 40 degrees Celsius of decolorations 30 minutes, the membrane filtration that then aperture is 1-5 μm takes filtrate, then Filtrate is obtained into concentrate through molecular cut off after 500Da.

It further illustrates, in step (3), the mulberry-leaf extract is the ethyl alcohol for the use of mass concentration being 15% by mulberry leaf Solution impregnates 5 minutes, and passes through microwave radiation exaraction simultaneously, and then microwave power recycles ethyl alcohol for 200-300W, by mulberry leaf 3 times of volume distilled water are added to carry out in U-tube heating and refluxing extraction 2 hours, takes filtrate, then continues to repeat filter residue Heating and refluxing extraction 2 hours, filtrate is taken, filtrate twice is merged, then adds the distilled water of 5 times of volumes, 0.1 times of volume Cellulase heat while stirring 10 minutes, then stop heating standing it is cooling after use three layers of filtered through gauze, take filtrate dense It is reduced to 1.12 times of elementary volume and then mulberry-leaf extract can be obtained after being spray-dried；The rice bran extract is by rice bran The distilled water of 4 times of volumes is added after cleaning by ultrasonic extraction 5 minutes, supersonic frequency 100-120KHZ, it is poly- to be subsequently added into shell Sugar stirring starts be separated by solid-liquid separation after standing within 1 minute to take filtrate, and the cellulose that weight ratio is 1:1-2:2-3 will be added in filtrate Enzyme, protease and amylase are hydrolyzed, and filtrate is then obtained by filtration, and matter is added after concentrating the filtrate to 1.01 times of original volume Measure the ethanol solution that concentration is 70% and stir and stand, via hole diameter is that 1-3 μm of membrane filtration goes filtrate, then by filtrate be passed through through It crosses the ion exchange column after 0.02mol/L phosphate buffer impregnates to be filtered, the flow velocity that filtrate is passed through ion exchange column is Then filtrate is obtained rice bran extract by freeze-drying or vacuum drying or forced air drying by 0.2-0.3mL/min.

It further illustrates, in step (3), the Rhodopseudomonas palustris, sour soil bacillus, lactobacillus acidophilus make Also pass through acclimation with preceding.

It further illustrates, the acclimation specific method of the Rhodopseudomonas palustris is: Rhodopseudomonas palustris is connect Kind in first domestication culture medium in 20-25 degrees Celsius culture 8-10 hour, then take by first tame cultivate after strain 0.1cm²25-30 degrees Celsius culture 8-10 hours in the second domestication culture medium are added, then take after the second domestication culture Strain 0.1cm²It is added in third domestication culture medium to cultivate 8-10 hours in 30-35 degrees Celsius and can screen the natural pond after being tamed Damp red pseudomonas；Wherein the formula of the first domestication culture medium is: sludge supernatant 200g/L, potassium dihydrogen phosphate 6g/L, nitric acid Sodium 5g/L, potassium chloride 3g/L, magnesium sulfate 2g/L, sucrose 30g/L, distilled water are settled to 1L；The formula of second domestication culture medium is: Sludge supernatant 400g/L, potassium dihydrogen phosphate 6g/L, sodium nitrate 5g/L, potassium chloride 3g/L, magnesium sulfate 2g/L, sucrose 30g/L steam Distilled water 1 is settled to L；The formula of third domestication culture medium is: sludge supernatant 800g/L, potassium dihydrogen phosphate 6g/L, sodium nitrate 5g/ L, potassium chloride 3g/L, magnesium sulfate 2g/L, sucrose 30g/L, distilled water 1 are settled to L.

Further illustrate, the acclimation specific method of the sour soil bacillus is: by sour soil bacillus be added by Magnesium chloride 0.5g/L, sodium bicarbonate 0.5g/L, sodium chloride 0.8g/L, yeast powder 8g/L, glucose 20g/L, distilled water 1L, pH= The activation culture of 6.5 compositions is shaking table culture 20-30 hours lower based on 29 degrees Celsius；Then the bacterium solution after culture out living is taken into 10mL Be added I domestication culture medium in it is shaking table culture 10-12 hour lower in 30 degrees Celsius, then will I domestication culture medium tame after It is shaking table culture 10-12 hours lower in 31 degrees Celsius in Section II domestication culture medium that bacterium solution takes 20mL to be added, and then tames Section II and trains It is shaking table culture 10-12 hours lower in 32 degrees Celsius in Section III domestication culture medium that bacterium solution after supporting base domestication takes 40mL to be added, then 60mL is taken to be added in Section IV domestication culture medium in 33 degrees Celsius of lower shaking table cultures the bacterium solution after Section III domestication culture medium domestication The sour soil bacillus after being tamed can be screened within 10-12 hours；Wherein the formula of I domestication culture medium is: alcohol waste residue Cleaning solution 100g/L, potassium dihydrogen phosphate 5g/L, ferric citrate 0.5g/L, sodium chloride 3g/L, microelement 0.6g/L, distilled water It is settled to 1L；The formula of Section II domestication culture medium is: alcohol waste residue cleaning solution 200g/L, potassium dihydrogen phosphate 5g/L, ironic citrate Ammonium 0.5g/L, sodium chloride 3g/L, microelement 0.6g/L, distilled water are settled to 1L；The formula of Section III domestication culture medium is: wine Smart waste residue cleaning solution 300g/L, potassium dihydrogen phosphate 5g/L, ferric citrate 0.5g/L, sodium chloride 3g/L, microelement 0.6g/L, Distilled water is settled to 1L；The formula of Section IV domestication culture medium is: alcohol waste residue cleaning solution 400g/L, potassium dihydrogen phosphate 5g/L, lemon Lemon acid iron ammonium 0.5g/L, sodium chloride 3g/L, microelement 0.6g/L, distilled water are settled to 1L.

It further illustrates, the acclimation specific method of the lactobacillus acidophilus is: the lactobacillus acidophilus of preservation is added To YDP slant medium culture 24-48 hours, then picking area is 0.1cm²Lawn be seeded to No.1 domestication culture medium In it is shaking table culture 12-15 hours lower in 25 degrees Celsius, then will No.1 tame culture medium in tame after bacterium solution take 0.1cm²It is added 28 degrees Celsius of shaking table culture 12-15 hours lower, bacterium solutions after then being tamed in No. two domestication culture mediums in No. two domestication culture mediums Take 0.1cm²Be added in No. three domestication culture mediums 32 degrees Celsius it is shaking table culture 12-15 hours lower, can screen after being tamed Lactobacillus acidophilus；Wherein, the formula of No.1 domestication culture medium is: sugar industry waste residue cleaning solution 100g/L, calcium chloride 3g/L, chlorination Potassium 3g/L, ammonium chloride 3g/L, ferric sulfate 3g/L, polysorbas20 mL, trace element solution 20mL, distilled water are settled to 1L；No. two are tamed and dociled The formula for changing culture medium is: sugar industry waste residue cleaning solution 300g/L, calcium chloride 3g/L, potassium chloride 3g/L, ammonium chloride 3g/L, sulfuric acid Iron 3g/L, polysorbas20 mL, trace element solution 20mL, distilled water are settled to 1L；The formula of No. three domestication culture mediums is: sugar industry Waste residue cleaning solution 900g/L, calcium chloride 3g/L, potassium chloride 3g/L, ammonium chloride 3g/L, ferric sulfate 3g/L, polysorbas20 mL, micro member Plain solution 20mL, distilled water are settled to 1L.

The preparation method of biological organic fertilizer is: in parts by weight, by the above-mentioned 5-10 parts by weight mulberry leaf being prepared The red spirillum of 1-5 parts by weight purple bacterium, 3-5 parts by weight marsh after extract, 5-10 parts by weight rice bran extract and domestication is red false single Born of the same parents bacterium, 3-5 parts by weight sour soil bacillus, 3-5 parts by weight lactobacillus acidophilus and 20-30 parts by weight peat, 20-30 parts by weight are sweet Maize pulp mixing after bagasse, 20-30 parts by weight fermentation alcohol builds heap and ferments 2-3 days.

In conclusion by adopting the above-described technical solution, the beneficial effects of the present invention are:

Thimerosal in the application passes through compound botanical extracting solution, has more beneficial Disinfection Effect, and hundred mattresses first are clear Metabolic capabilities by destroying fungal cell play the role of disinfection and sterilization, and adhesion is difficult to fall from kind of a block surface clean by force Residual rate is excessively high, bactericidal range limitation, and non-irrigated lotus root kind block, which digs out to clean from soil, saves as seed, and surface still can be containing big The thallus of amount can be sterilized using hundred mattress clear solutions, can be long in kind of the block appearance residence time after being sterilized using it, although can inhibit to have The formation of evil flora, but the time remains can enter in kind of block in the subsurface wastewater infiltration system with good humidity too long, influences the growth of kind of block Development and quality, kind block can also contain harmful thimerosal ingredient, and safety is not strong, and compounding salsola collina extracting solution, swainson pea mention Bactericidal range first after liquid, castor-oil plant extracting solution is taken to expand, they can inhibit the formation of different floras, but in plant extraction liquid The substance that is formed of inhibition flora be that the inhibition played of the substances such as vegetal alkaloid, volatile oil, ketone, soaping agents is made With remaining in kind of block appearance earthing after thimerosal after planting, under the conditions of good humidity and temperature, these substances are native Microbial bacteria after the domestication contained in biological organic fertilizer in earth is decomposed into small molecule carbon nitrogen source and is absorbed and utilized by kind of a block, and pig Hair dish extracting solution, swainson pea extracting solution, castor-oil plant extracting solution show alkalescent, can neutralize the clear acidity of hundred mattresses, and hundred mattresses of reduction are clear to be glued Put forth effort, mitigate hundred mattresses clearly because good environment adventicous species block inside a possibility that, and when make biological organic fertilizer through domestication Lactobacillus acidophilus and sour soil bacillus afterwards can be broken down into clearly small molecule carbon source using hundred mattresses and be supplied to kind of a block absorption It utilizes.Biological organic fertilizer is contained using the polysaccharide for being up to 95% or more in mulberry-leaf extract, rice bran extract containing purity, polysaccharide There is multiclass such as glucose, galactolipin, arabinose, mannose, uronic acid etc., by applicants have found that mentioning using mulberry leaf Take object, rice bran extract combination Rhodopseudomonas palustris, sour soil bacillus, lactobacillus acidophilus quickly can be grown to highest life Long curve, to decompose the raw materials such as the maize pulp after peat, bagasse, fermentation alcohol, quickly so as to shorten preparation biology The time of organic fertilizer finds that Rhodopseudomonas palustris, sour soil bacillus, lactobacillus acidophilus pass through special domestication after study The organic matter 5.32% that can will be released after the fermenting raw materials such as maize pulp after peat, bagasse, fermentation alcohol after combination, nitrogen 1.02%, phosphoric acid 0.34%, ammonium nitrogen 320ppm, nitrate nitrogen 9ppm, rapid available phosphorus 102ppm, available potassium 288ppm, and using should COD value of the combined zymocyte liquid in the peat after everfermentation reduce the COD value in 78%, bagasse reduce 82%, The COD value in maize pulp after fermentation alcohol reduces 86%, can utilize peat, bagasse, hair by new technological means The benefit materials contained in maize pulp after ferment alcohol, and the COD value of waste can be reduced, it avoids waste stacking from not handling and makes The phenomenon that at environmental pollution or abandoning waste available resources.

[specific embodiment]

In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below Embodiment is closed, the present invention is further explained.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, It is not intended to limit the present invention.

Embodiment 1:

B, site preparation: digging to field before passing the winter, and deep ploughs 25 centimetres when digging, dried the native wind-engaging sun climbed over In the winter, the upturned soil tedded deep ploughing when site preparation is thin, carefully harrows hack, makes that the soil is porous；

(2) seed treatment: non-irrigated lotus root seed of the selection without lesion of rotting, each drought lotus root seed is a section kind block, each kind of block Bud 2, band, kind block are carried out disinfection using thimerosal,

In parts by weight, including 1 part of hundred mattress is clear, 3 parts of salsola collina extracting solutions, 3 parts of swainson peas extract for the thimerosal Liquid, 3 parts of castor-oil plant extracting solutions, 20 parts of distilled water；

Hair dish extracting solution is added 75% ethyl alcohol of mass concentration after cleaning hair dish and carries out heating and refluxing extraction 30 minutes, filtering Filtrate is taken, 1.01 times of original volume are then concentrated into；

The swainson pea extracting solution is then skin of beancurd is removed to hot-water soak 5 minutes of 40 degrees Celsius of horsebean, then The water that 2 times of volumes are added is beaten, and 0.2 times of slurry weight of diatomite stirring is then added, and mass fraction is then added and is After 75% ethyl alcohol stirring stands 12 hours, by filtering to take filtrate；

Castor-oil plant extracting solution is after crushing castor leaf, and it is that 80% ethyl alcohol be heated to 70 degrees Celsius times that mass fraction, which is added, Stream extracts 3 times, 30 minutes every time, then filters to take filtrate, and 2 times of (v/v) water then are added in filtrate, and active carbon is then added and adds Heat is decolourized 30 minutes to 40 degrees Celsius, and then aperture is 1 μm of membrane filtration, filtrate is taken, then by filtrate through molecular cut off Concentrate is obtained after 500Da；

(3) it sows: the kind block after disinfection is sowed, a kind of block of each cave hole sowing, each cave hole is 25 centimetres wide, 20 centimetres deep, the cave hole held successfully first backfills 3 cm thicks that loosen the soil, and applies biological organic fertilizer 1.5kg later, then in biological organic The fertile loose fine earth for returning one layer of about 1 cm thick of lid above, dissipates biological organic fertilizer 0.5kg on loose fine earth, then return lid again later The loose fine earth of one layer of 5 cm thick then enters the kind block flicking after disinfection in fine earth, and the bud for planting block is without exception upward, finally uses Kind block is returned training leveling by moist soil, makes Hui Tuman lid kinds 2 centimetres of block, 100 centimetres of the line-spacing of sowing, and 100 centimetres of spacing in the rows；

The biological organic fertilizer includes that following raw material is made in parts by weight: 20 parts by weight peat, 20 parts by weight are sweet Maize pulp, 5 parts by weight mulberry-leaf extracts after bagasse, 20 parts by weight fermentation alcohols, 5 parts by weight rice bran extracts, 3 parts by weight natural ponds Damp red pseudomonas, 3 parts by weight sour soil bacillus, 3 parts by weight lactobacillus acidophilus；

Wherein, mulberry-leaf extract is by mulberry leaf using alcohol solution dipping 5 minutes that mass concentration is 15%, and is passed through simultaneously Microwave radiation exaraction is crossed, then microwave power recycles ethyl alcohol for 200W, 3 times of volume distilled water are added in U-tube in mulberry leaf It carries out heating and refluxing extraction 2 hours, takes filtrate, then continue to filter residue be repeated to heating and refluxing extraction 2 hours, take filtrate, Filtrate twice is merged, the cellulase of the distilled water, 0.1 times of volume that then add 5 times of volumes heats while stirring 10 Minute, then stop heating standing it is cooling after use three layers of filtered through gauze, take filtrate be concentrated into 1.12 times of elementary volume then into Mulberry-leaf extract can be obtained after row spray drying；The rice bran extract is the distillation that 4 times of volumes are added after cleaning rice bran Water was subsequently added into after chitosan stirring is stood for 1 minute by ultrasonic extraction 5 minutes, supersonic frequency 100KHZ and starts to be consolidated Liquid separation takes filtrate, and cellulase, protease and amylase that weight ratio is 1:1:2 will be added in filtrate and be hydrolyzed, then Filtrate is obtained by filtration, addition mass concentration is quiet for 70% ethanol solution stirring after concentrating the filtrate to 1.01 times of original volume It sets, via hole diameter is that 1 μm of membrane filtration goes filtrate, and then filtrate is passed through after the immersion of 0.02mol/L phosphate buffer Ion exchange column be filtered, the flow velocity that filtrate is passed through ion exchange column is 0.2mL/min, then that filtrate is dry by freezing Dry or vacuum drying or forced air drying obtain rice bran extract；

And Rhodopseudomonas palustris, sour soil bacillus, lactobacillus acidophilus are also passing through acclimation using preceding.

The acclimation specific method of the Rhodopseudomonas palustris is: Rhodopseudomonas palustris is inoculated in the first domestication It is cultivated 8 hours in culture medium in 20 degrees Celsius, then takes the strain 0.1cm after the first domestication culture²The second domestication is added It is cultivated 8 hours for 25 degrees Celsius in culture medium, then takes the strain 0.1cm after the second domestication culture²Third domestication training is added It supports to cultivate 8 hours in base in 30 degrees Celsius and can screen the Rhodopseudomonas palustris after being tamed；Wherein the first domestication culture The formula of base is: sludge supernatant 200g/L, potassium dihydrogen phosphate 6g/L, sodium nitrate 5g/L, potassium chloride 3g/L, magnesium sulfate 2g/L, Sucrose 30g/L, distilled water are settled to 1L；The formula of second domestication culture medium is: sludge supernatant 400g/L, potassium dihydrogen phosphate 6g/L, sodium nitrate 5g/L, potassium chloride 3g/L, magnesium sulfate 2g/L, sucrose 30g/L, distilled water 1 are settled to L；Third tames culture medium Formula be: sludge supernatant 800g/L, potassium dihydrogen phosphate 6g/L, sodium nitrate 5g/L, potassium chloride 3g/L, magnesium sulfate 2g/L, sugarcane Sugared 30g/L, distilled water 1 are settled to L；

The acclimation specific method of the sour soil bacillus is: sour soil bacillus is added by magnesium chloride 0.5g/ L, the work of sodium bicarbonate 0.5g/L, sodium chloride 0.8g/L, yeast powder 8g/L, glucose 20g/L, distilled water 1L, pH=6.5 composition Change culture and is based on 29 degrees Celsius lower shaking table culture 20 hours；Then take 10mL that I domestication training is added the bacterium solution after culture out living Support in base in 30 degrees Celsius lower shaking table culture 10 hours, then take 20mL to be added the bacterium solution after I domestication culture medium domestication II tame culture medium in 31 degrees Celsius lower shaking table culture 10 hours, then by Section II domestication culture medium domestication after bacterium solution take 40mL be added Section III domestication culture medium in 32 degrees Celsius lower shaking table culture 10 hours, then by Section III domestication culture medium domestication Bacterium solution afterwards takes to screen within 10 hours in 60mL addition Section IV domestication culture medium in 33 degrees Celsius of lower shaking table cultures and be tamed Sour soil bacillus afterwards；Wherein the formula of I domestication culture medium is: alcohol waste residue cleaning solution 100g/L, potassium dihydrogen phosphate 5g/ L, ferric citrate 0.5g/L, sodium chloride 3g/L, microelement 0.6g/L, distilled water are settled to 1L；Section II tames culture medium Formula is: alcohol waste residue cleaning solution 200g/L, potassium dihydrogen phosphate 5g/L, ferric citrate 0.5g/L, sodium chloride 3g/L, micro member Plain 0.6g/L, distilled water are settled to 1L；The formula of Section III domestication culture medium is: alcohol waste residue cleaning solution 300g/L, biphosphate Potassium 5g/L, ferric citrate 0.5g/L, sodium chloride 3g/L, microelement 0.6g/L, distilled water are settled to 1L；Section IV domestication culture The formula of base is: alcohol waste residue cleaning solution 400g/L, potassium dihydrogen phosphate 5g/L, ferric citrate 0.5g/L, sodium chloride 3g/L, micro- Secondary element 0.6g/L, distilled water are settled to 1L；

The acclimation specific method of the lactobacillus acidophilus is: the lactobacillus acidophilus of preservation being added to the inclined-plane YDP and is trained After supporting base culture 24 hours, then picking area is 0.1cm²Lawn be seeded to No.1 domestication culture medium under 25 degrees Celsius Shaking table culture 12 hours, then No.1 is tamed to bacterium solution after taming in culture medium take 0.1cm²It is added 28 in No. two domestication culture mediums Degrees Celsius lower shaking table culture 12 hours, bacterium solution took 0.1cm after then taming in No. two domestication culture mediums²No. three domestication trainings are added 32 degrees Celsius lower shaking table culture 12 hours in base are supported, the lactobacillus acidophilus after being tamed can be screened；Wherein, No.1 domestication training The formula for supporting base is: sugar industry waste residue cleaning solution 100g/L, calcium chloride 3g/L, potassium chloride 3g/L, ammonium chloride 3g/L, ferric sulfate 3g/L, polysorbas20 mL, trace element solution 20mL, distilled water are settled to 1L；The formula of No. two domestication culture mediums is: sugar industry is useless Slag cleaning solution 300g/L, calcium chloride 3g/L, potassium chloride 3g/L, ammonium chloride 3g/L, ferric sulfate 3g/L, polysorbas20 mL, microelement Solution 20mL, distilled water are settled to 1L；The formula of No. three domestication culture mediums is: sugar industry waste residue cleaning solution 900g/L, calcium chloride 3g/L, potassium chloride 3g/L, ammonium chloride 3g/L, ferric sulfate 3g/L, polysorbas20 mL, trace element solution 20mL, distilled water are settled to 1L；

The preparation method of biological organic fertilizer is: in parts by weight, by the above-mentioned mulberry-leaf extract being prepared, rice bran Rhodopseudomonas palustris, sour soil bacillus, lactobacillus acidophilus and part peat, bagasse, fermented wine after extract and domestication Maize pulp mixing after essence builds heap and ferments 3 days；

(4) field management: including weeding, fertilising, ridging and the prevention and control of plant diseases, pest control；And the field management in two stages into Row, after the first stage is non-irrigated lotus root kind block insemination and emergence, seedling carries out when 30 centimetres long；Second stage is August part of current year or blooms Preceding progress；

S2, fertilising: the first stage is cheated with the cave that every plant of non-irrigated lotus root kind block is sowed as center of radius, and radius is 5 centimetres and opens 5 lis deeply 30g urea then earthing is added in wide 5 centimetres of the ditch of rice；Second stage is cheated with the cave of every plant of non-irrigated lotus root kind block sowing as radius Center, radius is 5 centimetres and opens deep 5 centimetres wide 5 centimetres of ditch, applies 50g phosphorus potash fertilizer then earthing, then by cornstalk straw or rice Grass is averagely bedded in the left and right sides of non-irrigated lotus root root, fills in the fine lower liquid manure with field head manufacture of weather sun respectively pour row later In ditch, the liquid manure amount for filling leaching has seen water with ground, every two days again around the water-rich soil flip lid to non-irrigated lotus root root in ditch Cornstalk straw or straw on；

S3, ridging: to promote the growth of non-irrigated lotus root and preventing the big lodging of later period wind, growth period need to earth up 1 time；

Embodiment 2:

B, site preparation: digging to field before passing the winter, and deep ploughs 35 centimetres when digging, dried the native wind-engaging sun climbed over In the winter, the upturned soil tedded deep ploughing when site preparation is thin, carefully harrows hack, makes that the soil is porous；

(2) seed treatment: non-irrigated lotus root seed of the selection without lesion of rotting, each drought lotus root seed is a section kind block, each kind of block Bud 3, band, kind block are carried out disinfection using thimerosal,

In parts by weight, including 5 part of hundred mattress is clear, 8 parts of salsola collina extracting solutions, 8 parts of swainson peas extract for the thimerosal Liquid, 8 parts of castor-oil plant extracting solutions, 50 parts of distilled water；

Castor-oil plant extracting solution is after crushing castor leaf, and it is that 80% ethyl alcohol be heated to 70 degrees Celsius times that mass fraction, which is added, Stream extracts 3 times, 30 minutes every time, then filters to take filtrate, and 2 times of (v/v) water then are added in filtrate, and active carbon is then added and adds Heat is decolourized 30 minutes to 40 degrees Celsius, and then aperture is 5 μm of membrane filtration, filtrate is taken, then by filtrate through molecular cut off Concentrate is obtained after 500Da；

(3) it sows: the kind block after disinfection is sowed, 3 kinds of blocks of each cave hole sowing, each cave hole is 35 centimetres wide, 25 centimetres deep, the cave hole held successfully first backfills 5 cm thicks that loosen the soil, and applies biological organic fertilizer 2kg later, then in biological organic fertilizer The loose fine earth of one layer of about 3 cm thick of lid is returned above, dissipates biological organic fertilizer 1kg again later on loose fine earth, then returns one layer 7 of lid The loose fine earth of cm thick then enters the kind block flicking after disinfection in fine earth, and the bud for planting block is without exception upward, finally uses moist soil handle Kind of block returns training leveling, makes 3 centimetres of block of Hui Tuman lid kind, 120 centimetres of the line-spacing of sowing, and 110 centimetres of spacing in the rows；

The biological organic fertilizer includes that following raw material is made in parts by weight: 30 parts by weight peat, 30 parts by weight are sweet Maize pulp, 10 parts by weight mulberry-leaf extracts, 10 parts by weight rice bran extracts, 5 parts by weight after bagasse, 30 parts by weight fermentation alcohols Rhodopseudomonas palustris, 5 parts by weight sour soil bacillus, 5 parts by weight lactobacillus acidophilus；

Wherein, mulberry-leaf extract is by mulberry leaf using alcohol solution dipping 5 minutes that mass concentration is 15%, and is passed through simultaneously Microwave radiation exaraction is crossed, then microwave power recycles ethyl alcohol for 300W, 3 times of volume distilled water are added in U-tube in mulberry leaf It carries out heating and refluxing extraction 2 hours, takes filtrate, then continue to filter residue be repeated to heating and refluxing extraction 2 hours, take filtrate, Filtrate twice is merged, the cellulase of the distilled water, 0.1 times of volume that then add 5 times of volumes heats while stirring 10 Minute, then stop heating standing it is cooling after use three layers of filtered through gauze, take filtrate be concentrated into 1.12 times of elementary volume then into Mulberry-leaf extract can be obtained after row spray drying；The rice bran extract is the distillation that 4 times of volumes are added after cleaning rice bran Water was subsequently added into after chitosan stirring is stood for 1 minute by ultrasonic extraction 5 minutes, supersonic frequency 120KHZ and starts to be consolidated Liquid separation takes filtrate, and cellulase, protease and amylase that weight ratio is 1:2:3 will be added in filtrate and be hydrolyzed, then Filtrate is obtained by filtration, addition mass concentration is quiet for 70% ethanol solution stirring after concentrating the filtrate to 1.01 times of original volume It sets, via hole diameter is that 3 μm of membrane filtration goes filtrate, and then filtrate is passed through after the immersion of 0.02mol/L phosphate buffer Ion exchange column be filtered, the flow velocity that filtrate is passed through ion exchange column is 0.3mL/min, then that filtrate is dry by freezing Dry or vacuum drying or forced air drying obtain rice bran extract；

The acclimation specific method of the Rhodopseudomonas palustris is: Rhodopseudomonas palustris is inoculated in the first domestication It is cultivated 10 hours in culture medium in 25 degrees Celsius, then takes the strain 0.1cm after the first domestication culture²The second domestication is added It is cultivated 10 hours for 30 degrees Celsius in culture medium, then takes the strain 0.1cm after the second domestication culture²Third domestication training is added It supports to cultivate 10 hours in base in 35 degrees Celsius and can screen the Rhodopseudomonas palustris after being tamed；Wherein the first domestication culture The formula of base is: sludge supernatant 200g/L, potassium dihydrogen phosphate 6g/L, sodium nitrate 5g/L, potassium chloride 3g/L, magnesium sulfate 2g/L, Sucrose 30g/L, distilled water are settled to 1L；The formula of second domestication culture medium is: sludge supernatant 400g/L, potassium dihydrogen phosphate 6g/L, sodium nitrate 5g/L, potassium chloride 3g/L, magnesium sulfate 2g/L, sucrose 30g/L, distilled water 1 are settled to L；Third tames culture medium Formula be: sludge supernatant 800g/L, potassium dihydrogen phosphate 6g/L, sodium nitrate 5g/L, potassium chloride 3g/L, magnesium sulfate 2g/L, sugarcane Sugared 30g/L, distilled water 1 are settled to L；

The acclimation specific method of the sour soil bacillus is: sour soil bacillus is added by magnesium chloride 0.5g/ L, the work of sodium bicarbonate 0.5g/L, sodium chloride 0.8g/L, yeast powder 8g/L, glucose 20g/L, distilled water 1L, pH=6.5 composition Change culture and is based on 29 degrees Celsius lower shaking table culture 30 hours；Then take 10mL that I domestication training is added the bacterium solution after culture out living Support in base in 30 degrees Celsius lower shaking table culture 12 hours, then take 20mL to be added the bacterium solution after I domestication culture medium domestication II tame culture medium in 31 degrees Celsius lower shaking table culture 12 hours, then by Section II domestication culture medium domestication after bacterium solution take 40mL be added Section III domestication culture medium in 32 degrees Celsius lower shaking table culture 12 hours, then by Section III domestication culture medium domestication Bacterium solution afterwards takes to screen within 12 hours in 60mL addition Section IV domestication culture medium in 33 degrees Celsius of lower shaking table cultures and be tamed Sour soil bacillus afterwards；Wherein the formula of I domestication culture medium is: alcohol waste residue cleaning solution 100g/L, potassium dihydrogen phosphate 5g/ L, ferric citrate 0.5g/L, sodium chloride 3g/L, microelement 0.6g/L, distilled water are settled to 1L；Section II tames culture medium Formula is: alcohol waste residue cleaning solution 200g/L, potassium dihydrogen phosphate 5g/L, ferric citrate 0.5g/L, sodium chloride 3g/L, micro member Plain 0.6g/L, distilled water are settled to 1L；The formula of Section III domestication culture medium is: alcohol waste residue cleaning solution 300g/L, biphosphate Potassium 5g/L, ferric citrate 0.5g/L, sodium chloride 3g/L, microelement 0.6g/L, distilled water are settled to 1L；Section IV domestication culture The formula of base is: alcohol waste residue cleaning solution 400g/L, potassium dihydrogen phosphate 5g/L, ferric citrate 0.5g/L, sodium chloride 3g/L, micro- Secondary element 0.6g/L, distilled water are settled to 1L；

The acclimation specific method of the lactobacillus acidophilus is: the lactobacillus acidophilus of preservation being added to the inclined-plane YDP and is trained After supporting base culture 48 hours, then picking area is 0.1cm²Lawn be seeded to No.1 domestication culture medium under 25 degrees Celsius Shaking table culture 15 hours, then No.1 is tamed to bacterium solution after taming in culture medium take 0.1cm²It is added 28 in No. two domestication culture mediums Degrees Celsius lower shaking table culture 15 hours, bacterium solution took 0.1cm after then taming in No. two domestication culture mediums²No. three domestication trainings are added 32 degrees Celsius lower shaking table culture 15 hours in base are supported, the lactobacillus acidophilus after being tamed can be screened；Wherein, No.1 domestication training The formula for supporting base is: sugar industry waste residue cleaning solution 100g/L, calcium chloride 3g/L, potassium chloride 3g/L, ammonium chloride 3g/L, ferric sulfate 3g/L, polysorbas20 mL, trace element solution 20mL, distilled water are settled to 1L；The formula of No. two domestication culture mediums is: sugar industry is useless Slag cleaning solution 300g/L, calcium chloride 3g/L, potassium chloride 3g/L, ammonium chloride 3g/L, ferric sulfate 3g/L, polysorbas20 mL, microelement Solution 20mL, distilled water are settled to 1L；The formula of No. three domestication culture mediums is: sugar industry waste residue cleaning solution 900g/L, calcium chloride 3g/L, potassium chloride 3g/L, ammonium chloride 3g/L, ferric sulfate 3g/L, polysorbas20 mL, trace element solution 20mL, distilled water are settled to 1L；

The preparation method of biological organic fertilizer is: in parts by weight, by the above-mentioned mulberry-leaf extract being prepared, rice bran Rhodopseudomonas palustris, sour soil bacillus, lactobacillus acidophilus and peat, bagasse, fermentation alcohol after extract and domestication Maize pulp mixing afterwards builds heap and ferments 2 days；

(4) field management: including weeding, fertilising, ridging and the prevention and control of plant diseases, pest control；And the field management in two stages into Row, after the first stage is non-irrigated lotus root kind block insemination and emergence, seedling carries out when 40 centimetres long；Second stage is September part of current year or blooms Preceding progress；

S2, fertilising: the first stage is cheated with the cave that every plant of non-irrigated lotus root kind block is sowed as center of radius, and radius is 10 centimetres and opens deep 5 50g urea then earthing is added in centimetre wide 5 centimetres of ditch；Second stage is half with the cave hole of every plant of non-irrigated lotus root kind block sowing Diameter center, radius is 10 centimetres and opens deep 5 centimetres wide 5 centimetres of ditch, applies 80g phosphorus potash fertilizer then earthing, then by cornstalk straw Or straw is averagely bedded in the left and right sides of non-irrigated lotus root root, fills leaching respectively in the fine lower liquid manure with field head manufacture of weather sun later To in row ditch, the liquid manure amount for filling leaching has seen water with ground, every two days again the water-rich soil flip lid in ditch to non-irrigated lotus root root On the cornstalk straw or straw of surrounding；

S3, ridging: to promote the growth of non-irrigated lotus root and preventing the big lodging of later period wind, growth period need to earth up 2 times；

Embodiment 3:

B, site preparation: digging to field before passing the winter, and deep ploughs 30 centimetres when digging, dried the native wind-engaging sun climbed over In the winter, the upturned soil tedded deep ploughing when site preparation is thin, carefully harrows hack, makes that the soil is porous；

In parts by weight, including 3 part of hundred mattress is clear, 5 parts of salsola collina extracting solutions, 5 parts of swainson peas extract for the thimerosal Liquid, 5 parts of castor-oil plant extracting solutions, 40 parts of distilled water；

Castor-oil plant extracting solution is after crushing castor leaf, and it is that 80% ethyl alcohol be heated to 70 degrees Celsius times that mass fraction, which is added, Stream extracts 3 times, 30 minutes every time, then filters to take filtrate, and 2 times of (v/v) water then are added in filtrate, and active carbon is then added and adds Heat is decolourized 30 minutes to 40 degrees Celsius, and then aperture is 3 μm of membrane filtration, filtrate is taken, then by filtrate through molecular cut off Concentrate is obtained after 500Da；

(3) it sows: the kind block after disinfection is sowed, 2 kinds of blocks of each cave hole sowing, each cave hole is 29 centimetres wide, 22 centimetres deep, the cave hole held successfully first backfills 4 cm thicks that loosen the soil, and applies biological organic fertilizer 1.8kg later, then in biological organic The fertile loose fine earth for returning one layer of about 2 cm thick of lid above, dissipates biological organic fertilizer 0.8kg on loose fine earth, then return lid again later The loose fine earth of one layer of 6 cm thick then enters the kind block flicking after disinfection in fine earth, and the bud for planting block is without exception upward, finally uses Kind block is returned training leveling by moist soil, makes Hui Tuman lid kinds 2 centimetres of block, 110 centimetres of the line-spacing of sowing, and 105 centimetres of spacing in the rows；

The biological organic fertilizer includes that following raw material is made in parts by weight: 25 parts by weight peat, 25 parts by weight are sweet Maize pulp, 6 parts by weight mulberry-leaf extracts after bagasse, 25 parts by weight fermentation alcohols, 7 parts by weight rice bran extracts, 4 parts by weight natural ponds Damp red pseudomonas, 4 parts by weight sour soil bacillus, 4 parts by weight lactobacillus acidophilus；

Wherein, mulberry-leaf extract is by mulberry leaf using alcohol solution dipping 5 minutes that mass concentration is 15%, and is passed through simultaneously Microwave radiation exaraction is crossed, then microwave power recycles ethyl alcohol for 250W, 3 times of volume distilled water are added in U-tube in mulberry leaf It carries out heating and refluxing extraction 2 hours, takes filtrate, then continue to filter residue be repeated to heating and refluxing extraction 2 hours, take filtrate, Filtrate twice is merged, the cellulase of the distilled water, 0.1 times of volume that then add 5 times of volumes heats while stirring 10 Minute, then stop heating standing it is cooling after use three layers of filtered through gauze, take filtrate be concentrated into 1.12 times of elementary volume then into Mulberry-leaf extract can be obtained after row spray drying；The rice bran extract is the distillation that 4 times of volumes are added after cleaning rice bran Water was subsequently added into after chitosan stirring is stood for 1 minute by ultrasonic extraction 5 minutes, supersonic frequency 110KHZ and starts to be consolidated Liquid separation takes filtrate, and cellulase, protease and amylase that weight ratio is 1:1:3 will be added in filtrate and be hydrolyzed, then Filtrate is obtained by filtration, addition mass concentration is quiet for 70% ethanol solution stirring after concentrating the filtrate to 1.01 times of original volume It sets, via hole diameter is that 2 μm of membrane filtration goes filtrate, and then filtrate is passed through after the immersion of 0.02mol/L phosphate buffer Ion exchange column be filtered, filtrate be passed through ion exchange column flow velocity be 0.25mL/min, then by filtrate through freezing Dry or vacuum drying or forced air drying obtain rice bran extract；

The acclimation specific method of the Rhodopseudomonas palustris is: Rhodopseudomonas palustris is inoculated in the first domestication It is cultivated 9 hours in culture medium in 23 degrees Celsius, then takes the strain 0.1cm after the first domestication culture²The second domestication is added It is cultivated 9 hours for 28 degrees Celsius in culture medium, then takes the strain 0.1cm after the second domestication culture²Third domestication training is added It supports to cultivate 9 hours in base in 33 degrees Celsius and can screen the Rhodopseudomonas palustris after being tamed；Wherein the first domestication culture The formula of base is: sludge supernatant 200g/L, potassium dihydrogen phosphate 6g/L, sodium nitrate 5g/L, potassium chloride 3g/L, magnesium sulfate 2g/L, Sucrose 30g/L, distilled water are settled to 1L；The formula of second domestication culture medium is: sludge supernatant 400g/L, potassium dihydrogen phosphate 6g/L, sodium nitrate 5g/L, potassium chloride 3g/L, magnesium sulfate 2g/L, sucrose 30g/L, distilled water 1 are settled to L；Third tames culture medium Formula be: sludge supernatant 800g/L, potassium dihydrogen phosphate 6g/L, sodium nitrate 5g/L, potassium chloride 3g/L, magnesium sulfate 2g/L, sugarcane Sugared 30g/L, distilled water 1 are settled to L；

The acclimation specific method of the sour soil bacillus is: sour soil bacillus is added by magnesium chloride 0.5g/ L, the work of sodium bicarbonate 0.5g/L, sodium chloride 0.8g/L, yeast powder 8g/L, glucose 20g/L, distilled water 1L, pH=6.5 composition Change culture and is based on 29 degrees Celsius lower shaking table culture 25 hours；Then take 10mL that I domestication training is added the bacterium solution after culture out living Support in base in 30 degrees Celsius lower shaking table culture 11 hours, then take 20mL to be added the bacterium solution after I domestication culture medium domestication II tame culture medium in 31 degrees Celsius lower shaking table culture 11 hours, then by Section II domestication culture medium domestication after bacterium solution take 40mL be added Section III domestication culture medium in 32 degrees Celsius lower shaking table culture 11 hours, then by Section III domestication culture medium domestication Bacterium solution afterwards takes to screen within 11 hours in 60mL addition Section IV domestication culture medium in 33 degrees Celsius of lower shaking table cultures and be tamed Sour soil bacillus afterwards；Wherein the formula of I domestication culture medium is: alcohol waste residue cleaning solution 100g/L, potassium dihydrogen phosphate 5g/ L, ferric citrate 0.5g/L, sodium chloride 3g/L, microelement 0.6g/L, distilled water are settled to 1L；Section II tames culture medium Formula is: alcohol waste residue cleaning solution 200g/L, potassium dihydrogen phosphate 5g/L, ferric citrate 0.5g/L, sodium chloride 3g/L, micro member Plain 0.6g/L, distilled water are settled to 1L；The formula of Section III domestication culture medium is: alcohol waste residue cleaning solution 300g/L, biphosphate Potassium 5g/L, ferric citrate 0.5g/L, sodium chloride 3g/L, microelement 0.6g/L, distilled water are settled to 1L；Section IV domestication culture The formula of base is: alcohol waste residue cleaning solution 400g/L, potassium dihydrogen phosphate 5g/L, ferric citrate 0.5g/L, sodium chloride 3g/L, micro- Secondary element 0.6g/L, distilled water are settled to 1L；

The acclimation specific method of the lactobacillus acidophilus is: the lactobacillus acidophilus of preservation being added to the inclined-plane YDP and is trained After supporting base culture 32 hours, then picking area is 0.1cm²Lawn be seeded to No.1 domestication culture medium under 25 degrees Celsius Shaking table culture 14 hours, then No.1 is tamed to bacterium solution after taming in culture medium take 0.1cm²It is added 28 in No. two domestication culture mediums Degrees Celsius lower shaking table culture 14 hours, bacterium solution took 0.1cm after then taming in No. two domestication culture mediums²No. three domestication trainings are added 32 degrees Celsius lower shaking table culture 14 hours in base are supported, the lactobacillus acidophilus after being tamed can be screened；Wherein, No.1 domestication training The formula for supporting base is: sugar industry waste residue cleaning solution 100g/L, calcium chloride 3g/L, potassium chloride 3g/L, ammonium chloride 3g/L, ferric sulfate 3g/L, polysorbas20 mL, trace element solution 20mL, distilled water are settled to 1L；The formula of No. two domestication culture mediums is: sugar industry is useless Slag cleaning solution 300g/L, calcium chloride 3g/L, potassium chloride 3g/L, ammonium chloride 3g/L, ferric sulfate 3g/L, polysorbas20 mL, microelement Solution 20mL, distilled water are settled to 1L；The formula of No. three domestication culture mediums is: sugar industry waste residue cleaning solution 900g/L, calcium chloride 3g/L, potassium chloride 3g/L, ammonium chloride 3g/L, ferric sulfate 3g/L, polysorbas20 mL, trace element solution 20mL, distilled water are settled to 1L；

(4) field management: including weeding, fertilising, ridging and the prevention and control of plant diseases, pest control；And the field management in two stages into Row, after the first stage is non-irrigated lotus root kind block insemination and emergence, seedling carries out when 35 centimetres long；Second stage is carried out before the blooming of current year；

S2, fertilising: the first stage is cheated with the cave that every plant of non-irrigated lotus root kind block is sowed as center of radius, and radius is 8 centimetres and opens 5 lis deeply 40g urea then earthing is added in wide 5 centimetres of the ditch of rice；Second stage is cheated with the cave of every plant of non-irrigated lotus root kind block sowing as radius Center, radius is 8 centimetres and opens deep 5 centimetres wide 5 centimetres of ditch, applies 70g phosphorus potash fertilizer then earthing, then by cornstalk straw or rice Grass is averagely bedded in the left and right sides of non-irrigated lotus root root, fills in the fine lower liquid manure with field head manufacture of weather sun respectively pour row later In ditch, the liquid manure amount for filling leaching has seen water with ground, every two days again around the water-rich soil flip lid to non-irrigated lotus root root in ditch Cornstalk straw or straw on；

Comparative example 1:

Implantation methods are same as Example 1, and difference is the composition of thimerosal are as follows: 3 part of hundred mattress be clear, 40 parts of distilled water.

Comparative example 2:

Implantation methods are same as Example 1, and difference is that the biological organic fertilizer includes following original in parts by weight Material is made: maize pulp, 13 parts by weight brown sugar, 4 after 25 parts by weight peat, 25 parts by weight bagasse, 25 parts by weight fermentation alcohols Parts by weight Rhodopseudomonas palustris, 4 parts by weight sour soil bacillus, 4 parts by weight lactobacillus acidophilus.

Comparative example 3:

Implantation methods are same as Example 1, and difference is that the biological organic fertilizer includes following original in parts by weight Material is made: maize pulp, 7 parts by weight rice brans after 25 parts by weight peat, 25 parts by weight bagasse, 25 parts by weight fermentation alcohols extract Object, the red spirillum of 3 parts by weight purple bacterium, 4 parts by weight Rhodopseudomonas palustris, 4 parts by weight sour soil bacillus, 4 parts by weight acidophilus creams Bacillus, wherein Rhodopseudomonas palustris, sour soil bacillus, lactobacillus acidophilus are without acclimation and screening.

Comparative example 4:

Implantation methods are same as Example 1, and difference is that the biological organic fertilizer includes following original in parts by weight Material is made: maize pulp, 13 parts by weight brown sugar, 4 after 25 parts by weight peat, 25 parts by weight bagasse, 25 parts by weight fermentation alcohols Parts by weight Rhodopseudomonas palustris, 4 parts by weight sour soil bacillus, 4 parts by weight lactobacillus acidophilus, the wherein red false unit cell in marsh Bacterium, sour soil bacillus, lactobacillus acidophilus are without acclimation and screening.

Test 1: the planting patterns of embodiment 1-3 and comparative example 1-4 is cultivated into 77 mu of ground respectively and is tested, is distinguished per acre It is planted according to embodiment 1-3 with comparative example 1-4.Work as harvesting, record the heavy amount of harvesting per acre,

Table 1

	Yield (kg/hm²)
		Embodiment 1	86542.2
Embodiment 2	87261.4
		Embodiment 3	86842.5
Comparative example 1	76421.2
		Comparative example 2	78847.3
Comparative example 3	66426.1
		Comparative example 4	61947.5

As seen from the above table, using planting patterns (processing method, fertilizer etc.) of the invention the yield of non-irrigated lotus root is mentioned significantly It arrives, and is found during cultivation, the appearance pest and disease damage probability of embodiment 1-3 is small, and high survival rate, germinating time is fast, and tiller number Be also an important factor for increasing income more.

Test two:

Control group 1: Rhodopseudomonas palustris

Control group 2: sour soil bacillus

Control group 3: lactobacillus acidophilus

Control group 4: Rhodopseudomonas palustris, sour soil bacillus, lactobacillus acidophilus

Control group 5: Rhodopseudomonas palustris after domestication,

Control group 6: sour soil bacillus after domestication,

Control group 7: the lactobacillus acidophilus after domestication

Control group 8: the sour soil bacillus after Rhodopseudomonas palustris, domestication after domestication, the acidophilus cream bar after domestication Bacterium

Maize pulp after peat, bagasse, fermentation alcohol is filtered after the water of 5 times of weight is mixed and impregnated 10 hours Filtrate is taken, is respectively obtained peat cleaning solution (A), the maize pulp cleaning solution (C) after bagasse cleaning solution (B), fermentation alcohol, then COD value in three kinds of cleaning solutions, peat cleaning solution (A) COD value 4021.3mg/L, bagasse cleaning solution (B) COD value are detected respectively Maize pulp cleaning solution (C) COD value 6001.7mg/L after 5102.4mg/L, fermentation alcohol adds respectively in these three cleaning solutions The microbial bacteria for entering control group 1-8, according to the amount that 0.01g microbial bacteria is added in every 100mL, then in temperature at 30 degrees Celsius Lower shaking table culture detected COD value in every kind of cleaning solution after 15 hours, then COD removal rate in calculating.

The detection method of COD value is as follows:

Take 20mL water sample to be added in the conical flask of 500mL, then plus 0.025mol/L potassium bichromate solution 10mL and take few The mercuric sulfate solid of amount is added in conical flask and shakes up；It installs and is heated to reflux device, silver sulfate-sulfuric acid solution of 33mL is taken to flow back The addition of the top of pipe slowly begins to warm up reflux 2h after cooling several minutes of solution, stop heating and after solution is cooling It is slowly added into the distilled water of 89mL above condenser pipe, after solution is cooled to room temperature, 3 drop 1,10- phenanthroline indicators are added It is terminal that solution, which becomes bronzing through blue-green by yellow with the color that iron ammonium sulfate titrand is titrated to solution,.Note Record ml V1 of lower iron ammonium sulfate standard titration solution consumption；By identical step with 20.0mL distilled water substitute sample into Line blank experiment, other reagents and Specimen Determination method are all consistent, and consumed iron ammonium sulfate is titrated when recording blank Ml V2 of titrand.

COD (mg/L)=C1 (V2-V1) 8000/V0

In formula: the concentration (mol/L) of C1-- iron ammonium sulfate standard titration solution

The volume (ml) of ammonium ferric sulfate standard titration solution consumed by V2--- blank assay

The volume (ml) of consumed iron ammonium sulfate standard titration solution when V1--- Specimen Determination

The volume (ml) of V0--- sample

Scaled value of the molal weight of 8000-----1/4 oxygen as unit of mg/L.

COD removal rate=(cleaning solution COD value after cleaning solution COD value-reaction before reacting)/cleaning solution COD value before reacting, and Parallel testing three times is done in detection every time, is averaged.

Table 2

As seen from the above table, it is fermented using single strain, the effect for removing COD is not so good as the effect of three's compounded combination Fruit, strain of the single strain after special domestication has better removal ability, by three's strain compounding group after domestication The COD value closed in removal waste has synergistic effect.

The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously Limitation of the scope of the invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art, Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection model of the invention It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.

Claims

1. a kind of high-yield planting method of drought lotus root, it is characterised in that: following steps are specifically included,

B, site preparation: digging to field before passing the winter, and deep ploughs 25-35 centimetres when digging, dried the native wind-engaging sun climbed over In the winter, the upturned soil tedded deep ploughing when site preparation is thin, carefully harrows hack, makes that the soil is porous；

(2) seed treatment: non-irrigated lotus root seed of the selection without lesion of rotting, each drought lotus root seed are a section kind block, each kind of block band bud 2-3, kind block is carried out disinfection using thimerosal,

In parts by weight, including hundred mattress of 1-5 part is clear, 3-8 parts of salsola collina extracting solutions, 3-8 parts of swainson peas mention for the thimerosal Take liquid, 3-8 parts of castor-oil plant extracting solutions, 20-50 parts of distilled water；

(3) it sows: the kind block after disinfection is sowed, 1-3 kind of blocks of each cave hole sowing, each cave hole is 25-35 centimetres wide, Deep 20-25 centimetres, the cave hole held successfully first backfills the 3-5 cm thick that loosens the soil, and applies biological organic fertilizer 1.5-2kg later, then exists The loose fine earth that one layer of about 1-3 cm thick of lid is returned above biological organic fertilizer, dissipates biological organic fertilizer 0.5-1kg in loose fine earth again later On, then the loose fine earth of one layer of 5-7 cm thick of lid is returned, then the kind block flicking after disinfection is entered in fine earth, and plant the bud one of block Kind block upward, is finally returned training leveling with moist soil by rule, makes Hui Tuman lid kind 2-3 centimetres of block, 100-120 centimetres of the line-spacing of sowing, 100-110 centimetres of spacing in the rows；

The biological organic fertilizer includes that following raw material is made in parts by weight: 20-30 parts by weight peat, 20-30 parts by weight Maize pulp, 5-10 parts by weight mulberry-leaf extract, 5-10 parts by weight rice bran after bagasse, 20-30 parts by weight fermentation alcohol extract Object, 3-5 parts by weight Rhodopseudomonas palustris, 3-5 parts by weight sour soil bacillus, 3-5 parts by weight lactobacillus acidophilus；

(4) field management: including weeding, fertilising, ridging and the prevention and control of plant diseases, pest control；And the field management carries out in two stages, After first stage is non-irrigated lotus root kind block insemination and emergence, carried out when seedling is 30-40 centimetres long；Second stage is the 8-9 month of current year or opens Spend preceding progress；

S1, weeding: mode of the application herbicide in conjunction with artificial weeding carries out weeding, when applying herbicide herbicide not with drought Lotus root stem or blade are direct, guarantee on the ground in field without weeds；

S2, fertilising: the first stage is cheated with the cave that every plant of non-irrigated lotus root kind block is sowed as center of radius, and radius is 5-10 centimetres and opens 5 lis deeply 30-50g urea then earthing is added in wide 5 centimetres of the ditch of rice；Second stage is half with the cave hole of every plant of non-irrigated lotus root kind block sowing Diameter center, radius is 5-10 centimetres and opens deep 5 centimetres wide 5 centimetres of ditch, applies 50-80g phosphorus potash fertilizer then earthing, then by corn Stalk straw or straw are averagely bedded in the left and right sides of non-irrigated lotus root root, distinguish later in the fine lower liquid manure with field head manufacture of weather sun Filling pours Hang Gouli, and the liquid manure amount for filling leaching has seen water with ground, every two days again the water-rich soil flip lid in ditch to non-irrigated lotus root On cornstalk straw or straw around root；

S4, the prevention and control of plant diseases, pest control: being directed to bud rot, stem rot, and gram bacterium star or rotten ichor for spraying 500 times of dilution are prevented and treated； The ZhiBingLing(SIC) of 1000 times of dilution is sprayed for virosis or viral ichor is prevented and treated；Prevented for the rust application mould pulvis of star It controls；1000 times of liquid are diluted for budworm application 40% dimethoate emulsifiable concentrate of mass concentration to spray；Mass concentration is applied for any of several broadleaf plants insect parasite which hurts rice plant 90% trichlorfon technical dilutes 800 times of liquid and sprays.

2. a kind of high-yield planting method of non-irrigated lotus root according to claim 1, it is characterised in that: described to disappear in step (2) In parts by weight, including 3 part of hundred mattress is clear, 5 parts of salsola collina extracting solutions, 5 parts of swainson pea extracting solutions, 5 portions of castor-oil plants extract for venom Liquid, 40 parts of distilled water.

3. a kind of high-yield planting method of non-irrigated lotus root according to claim 1, it is characterised in that: in step (3), the mulberry Leaf extract is by mulberry leaf using alcohol solution dipping 5 minutes that mass concentration is 15%, and passes through microwave radiation exaraction simultaneously, Then microwave power recycles ethyl alcohol for 200-300W, mulberry leaf 3 times of volume distilled water of addition are heated back in U-tube Stream extracts 2 hours, takes filtrate, then continues to filter residue be repeated to heating and refluxing extraction 2 hours, take filtrate, by filter twice Liquid merges, and the cellulase of the distilled water, 0.1 times of volume that then add 5 times of volumes heats while stirring 10 minutes, then stops Only heating uses three layers of filtered through gauze after standing cooling, after taking filtrate to be concentrated into 1.12 times of elementary volume and then be spray-dried Mulberry-leaf extract can be obtained；The rice bran extract is that the distilled water of 4 times of volumes of addition after cleaning rice bran is mentioned by ultrasound It takes 5 minutes, supersonic frequency 100-120KHZ, is subsequently added into and starts be separated by solid-liquid separation after chitosan stirring is stood for 1 minute to take Filtrate will be added cellulase, protease and amylase that weight ratio is 1:1-2:2-3 and be hydrolyzed, then filters in filtrate Filtrate is obtained, the ethanol solution stirring standing that mass concentration is 70%, warp is added after concentrating the filtrate to 1.01 times of original volume Aperture is that 1-3 μm of membrane filtration goes filtrate, then by filtrate be passed through after the immersion of 0.02mol/L phosphate buffer from Sub- exchange column is filtered, and the flow velocity that filtrate is passed through ion exchange column is 0.2-0.3mL/min, then that filtrate is dry by freezing Dry or vacuum drying or forced air drying obtain rice bran extract.

4. a kind of high-yield planting method of non-irrigated lotus root according to claim 1, it is characterised in that: in step (3), the natural pond Damp red pseudomonas, sour soil bacillus, lactobacillus acidophilus are also passing through acclimation using preceding.

5. a kind of high-yield planting method of non-irrigated lotus root according to claim 4, it is characterised in that: the Rhodopseudomonas palustris Acclimation specific method be: by Rhodopseudomonas palustris be inoculated in the first domestication culture medium in 20-25 degrees Celsius cultivate 8-10 hours, then take the strain 0.1cm after the first domestication culture²25-30 degrees Celsius is added in the second domestication culture medium Culture 8-10 hours then takes the strain 0.1cm after the second domestication culture²It is added in third domestication culture medium in 30-35 Degree Celsius culture can screen the Rhodopseudomonas palustris after being tamed in 8-10 hours；The wherein formula of the first domestication culture medium It is: sludge supernatant 200g/L, potassium dihydrogen phosphate 6g/L, sodium nitrate 5g/L, potassium chloride 3g/L, magnesium sulfate 2g/L, sucrose 30g/ L, distilled water are settled to 1L；The formula of second domestication culture medium is: sludge supernatant 400g/L, potassium dihydrogen phosphate 6g/L, nitric acid Sodium 5g/L, potassium chloride 3g/L, magnesium sulfate 2g/L, sucrose 30g/L, distilled water 1 are settled to L；The formula of third domestication culture medium is: Sludge supernatant 800g/L, potassium dihydrogen phosphate 6g/L, sodium nitrate 5g/L, potassium chloride 3g/L, magnesium sulfate 2g/L, sucrose 30g/L steam Distilled water 1 is settled to L.

6. a kind of high-yield planting method of non-irrigated lotus root according to claim 4, it is characterised in that: the sour soil bacillus Acclimation specific method is: sour soil bacillus is added by magnesium chloride 0.5g/L, sodium bicarbonate 0.5g/L, sodium chloride 0.8g/L, yeast powder 8g/L, glucose 20g/L, distilled water 1L, the activation culture that pH=6.5 is formed are based on shaking under 29 degrees Celsius Bed culture 20-30 hours；Then it takes 10mL to be added in I domestication culture medium the bacterium solution after culture out living to shake under 30 degrees Celsius Then bacterium solution after I domestication culture medium domestication is taken 20mL to be added in Section II domestication culture medium in 31 by bed culture 10-12 hours Degrees Celsius lower shaking table culture 10-12 hours, then take 40mL that Section III domestication is added the bacterium solution after Section II domestication culture medium domestication It is shaking table culture 10-12 hours lower in 32 degrees Celsius in culture medium, the bacterium solution after Section III domestication culture medium domestication is then taken into 60mL It is added in Section IV domestication culture medium in 33 degrees Celsius of lower shaking table culture 10-12 hours sour soil gemma that can be screened after being tamed Bacillus；Wherein the formula of I domestication culture medium is: alcohol waste residue cleaning solution 100g/L, potassium dihydrogen phosphate 5g/L, ferric citrate 0.5g/L, sodium chloride 3g/L, microelement 0.6g/L, distilled water are settled to 1L；The formula of Section II domestication culture medium is: alcohol is useless Slag cleaning solution 200g/L, potassium dihydrogen phosphate 5g/L, ferric citrate 0.5g/L, sodium chloride 3g/L, microelement 0.6g/L, distillation Water is settled to 1L；The formula of Section III domestication culture medium is: alcohol waste residue cleaning solution 300g/L, potassium dihydrogen phosphate 5g/L, citric acid Iron ammonium 0.5g/L, sodium chloride 3g/L, microelement 0.6g/L, distilled water are settled to 1L；The formula of Section IV domestication culture medium is: wine Smart waste residue cleaning solution 400g/L, potassium dihydrogen phosphate 5g/L, ferric citrate 0.5g/L, sodium chloride 3g/L, microelement 0.6g/L, Distilled water is settled to 1L.

7. a kind of high-yield planting method of non-irrigated lotus root according to claim 4, it is characterised in that: the lactobacillus acidophilus is tamed and dociled Changing processing specific method is: the lactobacillus acidophilus of preservation being added to YDP slant medium culture 24-48 hours, is then chosen Taking area is 0.1cm²Lawn be seeded to No.1 domestication culture medium in it is shaking table culture 12-15 hours lower in 25 degrees Celsius, then No.1 is tamed to bacterium solution after taming in culture medium and takes 0.1cm²28 degrees Celsius of lower shaking table culture 12- in No. two domestication culture mediums are added 15 hours, bacterium solution took 0.1cm after then taming in No. two domestication culture mediums²It is added in No. three domestication culture mediums under 32 degrees Celsius Shaking table culture 12-15 hours, the lactobacillus acidophilus after being tamed can be screened；Wherein, the formula of No.1 domestication culture medium is: Sugar industry waste residue cleaning solution 100g/L, calcium chloride 3g/L, potassium chloride 3g/L, ammonium chloride 3g/L, ferric sulfate 3g/L, polysorbas20 mL, Trace element solution 20mL, distilled water are settled to 1L；The formula of No. two domestication culture mediums is: sugar industry waste residue cleaning solution 300g/ L, calcium chloride 3g/L, potassium chloride 3g/L, ammonium chloride 3g/L, ferric sulfate 3g/L, polysorbas20 mL, trace element solution 20mL, distillation Water is settled to 1L；The formula of No. three domestication culture mediums is: sugar industry waste residue cleaning solution 900g/L, calcium chloride 3g/L, potassium chloride 3g/ L, ammonium chloride 3g/L, ferric sulfate 3g/L, polysorbas20 mL, trace element solution 20mL, distilled water are settled to 1L.