CN109640645B - 用于治疗疾病的遗传修饰的细胞、组织和器官 - Google Patents

用于治疗疾病的遗传修饰的细胞、组织和器官 Download PDF

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CN109640645B
CN109640645B CN201780049966.6A CN201780049966A CN109640645B CN 109640645 B CN109640645 B CN 109640645B CN 201780049966 A CN201780049966 A CN 201780049966A CN 109640645 B CN109640645 B CN 109640645B
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cells
genetically modified
human
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cell
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CN109640645A (zh
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伯尔哈德·J·赫林
克里斯多佛·布尔拉克
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Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10967298B2 (en) 2012-03-15 2021-04-06 Flodesign Sonics, Inc. Driver and control for variable impedence load
US9950282B2 (en) 2012-03-15 2018-04-24 Flodesign Sonics, Inc. Electronic configuration and control for acoustic standing wave generation
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US10231817B2 (en) 2013-09-24 2019-03-19 Giner Life Sciences, Inc. System for gas treatment of a cell implant
CA2935960C (en) 2014-01-08 2023-01-10 Bart Lipkens Acoustophoresis device with dual acoustophoretic chamber
AU2015360502A1 (en) 2014-12-10 2017-06-29 Regents Of The University Of Minnesota Genetically modified cells, tissues, and organs for treating disease
US11021699B2 (en) 2015-04-29 2021-06-01 FioDesign Sonics, Inc. Separation using angled acoustic waves
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
CN110494543A (zh) 2016-10-19 2019-11-22 弗洛设计声能学公司 通过声学的亲和细胞提取
US11033666B2 (en) 2016-11-15 2021-06-15 Giner Life Sciences, Inc. Percutaneous gas diffusion device suitable for use with a subcutaneous implant
WO2018204867A1 (en) 2017-05-04 2018-11-08 Giner, Inc. Robust, implantable gas delivery device and methods, systems and devices including same
KR20220066413A (ko) 2017-12-14 2022-05-24 프로디자인 소닉스, 인크. 음향 트랜스듀서 구동기 및 제어기
EP4417212A3 (en) 2018-10-19 2024-12-04 Regents Of The University Of Minnesota Transplant tolerance induction with carbodiimide treated tolerizing vaccine
EP3760718A1 (en) * 2019-07-04 2021-01-06 Medizinische Hochschule Hannover Tissue for use as allogeneic or xenogeneic transplant and method for its production
CN111264469B (zh) * 2020-02-27 2022-03-25 西安交通大学医学院第一附属医院 一种基因免疫诱导的甲状腺相关性眼病动物模型的构建方法及雷帕霉素类药物的应用
WO2022005463A1 (en) * 2020-06-30 2022-01-06 National Yang-Ming University A glia cell and neuron co-culture system and method
CN111500693B (zh) * 2020-07-02 2020-09-22 中南大学 检测巨噬细胞中rfx1表达水平的试剂在制备巨噬细胞分型检测制剂中的应用
EP4380627A1 (en) * 2021-08-06 2024-06-12 Rutgers, the State University of New Jersey Method for producing genetically modified cells
EP4384544A1 (en) * 2021-08-11 2024-06-19 Sana Biotechnology, Inc. Genetically modified cells for allogeneic cell therapy
CN114958768B (zh) * 2022-06-02 2023-03-24 健颐生物科技发展(山东)有限公司 Fgf10旁分泌通用型人成纤维细胞制剂的制备方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6156306A (en) * 1994-08-17 2000-12-05 Albert Einstein College Of Medicine Of Yeshiva University Pancreatic β-cells for allogeneic transplantation without immunosuppression
CN1407851A (zh) * 1999-11-19 2003-04-02 韩国生命工学研究院 一种改进的通过体细胞核转移制备猪克隆胚胎的方法
CN1486365A (zh) * 2000-11-17 2004-03-31 异种(人类)免疫球蛋白在克隆转基因有蹄类动物中的表达
CN104046593A (zh) * 2013-03-14 2014-09-17 浙江大学 一种低免疫原性的人细胞及其制备方法
CN104789592A (zh) * 2015-03-19 2015-07-22 中国食品药品检定研究院 一种制备α-1,3GT基因敲除的非人哺乳动物的方法及应用
CN105612176A (zh) * 2012-07-31 2016-05-25 伊斯凯普治疗有限公司 Hla g修饰的细胞及方法

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5770201A (en) 1994-12-23 1998-06-23 Rijsuniversiteit Te Leiden HA-2 antigenic peptide
AU723003C (en) * 1995-08-04 2004-01-29 General Hospital Corporation, The Transgenic swine and swine cells having human HLA genes
JP4751493B2 (ja) 1996-03-20 2011-08-17 ブリストル−マイヤーズ スクイッブ カンパニー Gp39/cd40およびctla4/cd28/b7経路のブロックによって免疫反応を阻止する方法およびそれに使用する組成物
FR2760023B1 (fr) 1997-02-21 2004-05-07 Commissariat Energie Atomique Cellules eucaryotes exprimant a leur surface au moins une isoforme d'hla-g et leurs applications
EP1241931A4 (en) * 1999-09-13 2004-12-29 Univ Massachusetts CLONING PIGS USING DONORATION CELLS OR NUCLEAR CELLS OF DIFFERENTIATED CELLS AND PRODUCING PLURIPOTENT PIGS
CA2438501C (en) * 2001-02-14 2014-09-16 Leo T. Furcht Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof
WO2003054150A2 (en) * 2001-12-07 2003-07-03 The Ohio State University Research Foundation Apoptotic ebv-transformed lymphocytes, a therapeutic agent for post-transplant lymphoproliferative disorder
US20050123525A1 (en) 2003-11-13 2005-06-09 Ulrich Martin Composition and method for inducing immune tolerance towards cell, tissue and/or organ transplants
KR20090068646A (ko) * 2007-12-24 2009-06-29 (주)엠젠 Hla-g 유전자 및 daf 유전자를 발현하는 형질전환복제 돼지 및 그의 제조 방법
KR20100022684A (ko) * 2008-08-20 2010-03-03 (주)엠젠 세 개의 유전자를 발현하는 복합형질전환 복제 돼지 및 그의 제조 방법
WO2010037408A1 (en) 2008-09-30 2010-04-08 Curevac Gmbh Composition comprising a complexed (m)rna and a naked mrna for providing or enhancing an immunostimulatory response in a mammal and uses thereof
EP2184297A1 (en) 2008-11-07 2010-05-12 Hla-G Technologies HLA-G polypeptides and pharmaceutical uses thereof
EP2184070A1 (en) 2008-11-07 2010-05-12 Hla-G Technologies HLA-G proteins and pharmaceutical uses thereof
CA2745376A1 (en) * 2008-12-01 2010-06-10 Lifespan Extension Llc Methods and compositions for altering health, wellbeing, and lifespan
KR20170125406A (ko) 2009-08-11 2017-11-14 상가모 테라퓨틱스, 인코포레이티드 표적화 변형에 대한 동형접합성 유기체
AU2010282239A1 (en) * 2009-08-14 2012-03-08 Revivicor, Inc. Multi-transgenic pigs for diabetes treatment
US8734786B2 (en) * 2009-09-16 2014-05-27 Northwestern University Use of ECDI-fixed cell tolerance as a method for preventing allograft rejection
WO2012009337A2 (en) * 2010-07-12 2012-01-19 Dana-Farber Cancer Institute, Inc. Nlrc5 as a target to intervene mhc class 1-mediated immune responses
US20140045915A1 (en) * 2010-08-31 2014-02-13 The General Hospital Corporation Cancer-related biological materials in microvesicles
WO2012072096A1 (en) 2010-12-03 2012-06-07 Biontech Ag Method for cellular rna expression
NZ759163A (en) * 2011-02-14 2023-03-31 Revivicor Inc Genetically modified pigs for xenotransplantation of vascularized xenografts and derivatives thereof
EP3450563A1 (en) 2011-02-25 2019-03-06 Recombinetics, Inc. Genetically modified animals and methods for making the same
CN102911270A (zh) * 2011-08-05 2013-02-06 浙江大学 一种抑制移植器官慢性排斥反应的抗体的制备及纯化方法
KR102135601B1 (ko) * 2012-03-29 2020-07-20 더 트러스티스 오브 콜롬비아 유니버시티 인 더 시티 오브 뉴욕 탈모 질환의 치료 방법
WO2013188358A1 (en) * 2012-06-12 2013-12-19 The General Hospital Corporation Miniature swine transgenic for one or more coagulation factors
US20140115728A1 (en) * 2012-10-24 2014-04-24 A. Joseph Tector Double knockout (gt/cmah-ko) pigs, organs and tissues
UY35468A (es) 2013-03-16 2014-10-31 Novartis Ag Tratamiento de cáncer utilizando un receptor quimérico de antígeno anti-cd19
CA2928635C (en) * 2013-11-28 2022-06-21 Horizon Genomics Gmbh Somatic haploid human cell line
US20160060655A1 (en) * 2014-05-30 2016-03-03 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods to treat latent viral infections
SG10201911411YA (en) * 2014-06-26 2020-02-27 Regeneron Pharma Methods and compositions for targeted genetic modifications and methods of use
CN105586389A (zh) * 2014-10-21 2016-05-18 天津华大基因科技有限公司 试剂盒及其在检测遗传性骨病基因中的用途
BR112017008251B1 (pt) * 2014-10-22 2022-02-22 Indiana University Research And Technology Corporation Usos de um tecido de um porco transgênico que compreende um gene a(1,3) galactosiltransferase, cmah e ß4galnt2 interrompido e de um produto relacionado com a pele, e, métodos para preparação de um material de transplante para xenoenxerto, para produção de um composto de interesse e para produzir um reagente de cultura de células
AU2015360502A1 (en) * 2014-12-10 2017-06-29 Regents Of The University Of Minnesota Genetically modified cells, tissues, and organs for treating disease

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6156306A (en) * 1994-08-17 2000-12-05 Albert Einstein College Of Medicine Of Yeshiva University Pancreatic β-cells for allogeneic transplantation without immunosuppression
CN1407851A (zh) * 1999-11-19 2003-04-02 韩国生命工学研究院 一种改进的通过体细胞核转移制备猪克隆胚胎的方法
CN1486365A (zh) * 2000-11-17 2004-03-31 异种(人类)免疫球蛋白在克隆转基因有蹄类动物中的表达
CN105612176A (zh) * 2012-07-31 2016-05-25 伊斯凯普治疗有限公司 Hla g修饰的细胞及方法
CN104046593A (zh) * 2013-03-14 2014-09-17 浙江大学 一种低免疫原性的人细胞及其制备方法
CN104789592A (zh) * 2015-03-19 2015-07-22 中国食品药品检定研究院 一种制备α-1,3GT基因敲除的非人哺乳动物的方法及应用

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
"Suppression of human macrophage-mediated cytotoxicity by transgenic swine endothelial cell expression of HLA-G";Emilio L.Esquivel 等;《Transplant Immunology》;20150103;第32卷(第2期);第109-115页 *

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