CN109608535A - 一种优化的鸡α干扰素肽链及其重组表达工程菌株 - Google Patents
一种优化的鸡α干扰素肽链及其重组表达工程菌株 Download PDFInfo
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- CN109608535A CN109608535A CN201811642869.0A CN201811642869A CN109608535A CN 109608535 A CN109608535 A CN 109608535A CN 201811642869 A CN201811642869 A CN 201811642869A CN 109608535 A CN109608535 A CN 109608535A
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- alpha interferon
- chicken alpha
- pichia pastoris
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/56—IFN-alpha
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
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- Proteomics, Peptides & Aminoacids (AREA)
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- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
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CN201811642869.0A CN109608535B (zh) | 2018-12-29 | 2018-12-29 | 一种优化的鸡α干扰素肽链及其重组表达工程菌株 |
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CN201811642869.0A CN109608535B (zh) | 2018-12-29 | 2018-12-29 | 一种优化的鸡α干扰素肽链及其重组表达工程菌株 |
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CN109608535A true CN109608535A (zh) | 2019-04-12 |
CN109608535B CN109608535B (zh) | 2022-05-27 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112175065A (zh) * | 2020-10-10 | 2021-01-05 | 石家庄石牧药业有限公司 | 一种制备强活性、高表达量的重组鸡α干扰素新工艺 |
Citations (7)
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---|---|---|---|---|
CN1594568A (zh) * | 2004-06-24 | 2005-03-16 | 南京农业大学 | 一种重组鸡α干扰素的生产方法及其重组载体 |
CN101338314A (zh) * | 2008-06-16 | 2009-01-07 | 南京农业大学 | 一种重组鸡α干扰素基因及其重组载体 |
CN101928711A (zh) * | 2010-03-16 | 2010-12-29 | 南京农业大学 | 全基因合成鸡α干扰素基因及蛋白表达 |
CN101948845A (zh) * | 2010-09-01 | 2011-01-19 | 中国科学院微生物研究所 | 编码鸡α干扰素的优化基因及其在制备鸡α干扰素中的应用 |
CN102121013A (zh) * | 2009-11-27 | 2011-07-13 | 东北农业大学 | 可高效表达的成熟鸡α干扰素基因及其多肽的制备方法 |
CN106399321A (zh) * | 2016-08-27 | 2017-02-15 | 华南农业大学 | 一种制备融合表达重组鸡α干扰素的生产工艺 |
CN108220214A (zh) * | 2017-12-27 | 2018-06-29 | 华南农业大学 | 一种高效表达重组鸡α干扰素的工程菌 |
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2018
- 2018-12-29 CN CN201811642869.0A patent/CN109608535B/zh active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1594568A (zh) * | 2004-06-24 | 2005-03-16 | 南京农业大学 | 一种重组鸡α干扰素的生产方法及其重组载体 |
CN101338314A (zh) * | 2008-06-16 | 2009-01-07 | 南京农业大学 | 一种重组鸡α干扰素基因及其重组载体 |
CN102121013A (zh) * | 2009-11-27 | 2011-07-13 | 东北农业大学 | 可高效表达的成熟鸡α干扰素基因及其多肽的制备方法 |
CN101928711A (zh) * | 2010-03-16 | 2010-12-29 | 南京农业大学 | 全基因合成鸡α干扰素基因及蛋白表达 |
CN101948845A (zh) * | 2010-09-01 | 2011-01-19 | 中国科学院微生物研究所 | 编码鸡α干扰素的优化基因及其在制备鸡α干扰素中的应用 |
CN106399321A (zh) * | 2016-08-27 | 2017-02-15 | 华南农业大学 | 一种制备融合表达重组鸡α干扰素的生产工艺 |
CN108220214A (zh) * | 2017-12-27 | 2018-06-29 | 华南农业大学 | 一种高效表达重组鸡α干扰素的工程菌 |
Non-Patent Citations (5)
Title |
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KOTI SREEKRISHNA ET AL.: "Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastorid", 《GENE》 * |
MARGARET J. SEKELLICK ET AL.: "Chicken Interferon Gene: Cloning, Expression, and Analysis", 《JOURNAL OF INTERFERON RESEARCH》 * |
曹丁等: "鸡α-干扰素真核表达载体的改造及在毕赤酵母中的分泌表达", 《江苏农业科学》 * |
董亚青等: "鸡α-干扰素在毕赤酵母中的表达及其表达条件优化", 《江苏农业科学》 * |
陈红英等: "3个品种鸡α干扰素基因的克隆与序列分析", 《云南农业大学学报》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112175065A (zh) * | 2020-10-10 | 2021-01-05 | 石家庄石牧药业有限公司 | 一种制备强活性、高表达量的重组鸡α干扰素新工艺 |
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