CN104524564A - 一种鲫鱼疱疹病毒病复合疫苗制剂及制备方法和应用 - Google Patents
一种鲫鱼疱疹病毒病复合疫苗制剂及制备方法和应用 Download PDFInfo
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Abstract
Description
ddH2O | 29μL |
10×buffer J | 10μL |
质粒DNA(0.4μg/μL) | 50μL |
SacⅠ | 10μL |
BSA | 1μL |
实验组 | 接种物 | 剂量 | 数量 |
A组 | 鲫鱼疱疹病毒病复合疫苗制剂(本发明研制) | 100微升 | 90尾 |
B组 | 鲫鱼疱疹病毒病复合疫苗制剂(本发明研制) | 200微升 | 90尾 |
C组 | 鲫鱼疱疹病毒病复合疫苗制剂(本发明研制) | 300微升 | 90尾 |
D组 | 阳性对照(鲤疱疹病毒Ⅱ型细胞灭活疫苗) | 100微升 | 90尾 |
E组 | 生理盐水对照 | 100微升 | 90尾 |
实验组 | 接种物 | 剂量 | 数量 |
A组 | 鲫鱼疱疹病毒病复合疫苗制剂(本发明研制) | 100微升 | 56尾 |
B组 | 鲫鱼疱疹病毒病复合疫苗制剂(本发明研制) | 200微升 | 57尾 |
C组 | 鲫鱼疱疹病毒病复合疫苗制剂(本发明研制) | 300微升 | 66尾 |
D组 | 阳性对照(鲤疱疹病毒Ⅱ型细胞灭活疫苗) | 100微升 | 50尾 |
E组 | 生理盐水对照 | 100微升 | 20尾 |
Claims (7)
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105755014A (zh) * | 2016-03-23 | 2016-07-13 | 中国科学院水生生物研究所 | CaHV-TNFR特异基因及应用 |
CN108743933A (zh) * | 2018-05-29 | 2018-11-06 | 苏州大学 | 基于酵母表达载体的蛋白微晶包埋鲤疱疹病毒ii型-草鱼出血病毒亚单位疫苗的构建方法 |
CN110452926A (zh) * | 2019-08-07 | 2019-11-15 | 西北农林科技大学 | 一种展示CyHV-2膜蛋白的重组杆状病毒及其制备方法和应用 |
CN110468111A (zh) * | 2019-08-07 | 2019-11-19 | 西北农林科技大学 | 一种展示CyHV-2膜蛋白的重组杆状病毒及其制备方法和应用 |
CN110564750A (zh) * | 2019-08-30 | 2019-12-13 | 中国水产科学研究院长江水产研究所 | 一种鲫造血器官坏死症酵母口服疫苗及应用 |
CN112279896A (zh) * | 2018-11-05 | 2021-01-29 | 共鳞实业(深圳)有限公司 | 一种用于鱼类预防或治疗CyHV-2感染的试剂及应用 |
CN113925961A (zh) * | 2021-10-14 | 2022-01-14 | 武汉科前生物股份有限公司 | 一种酵母来源的副猪嗜血杆菌hps06257蛋白亚单位疫苗制备方法及应用 |
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CN105755014A (zh) * | 2016-03-23 | 2016-07-13 | 中国科学院水生生物研究所 | CaHV-TNFR特异基因及应用 |
CN108743933A (zh) * | 2018-05-29 | 2018-11-06 | 苏州大学 | 基于酵母表达载体的蛋白微晶包埋鲤疱疹病毒ii型-草鱼出血病毒亚单位疫苗的构建方法 |
CN112279896A (zh) * | 2018-11-05 | 2021-01-29 | 共鳞实业(深圳)有限公司 | 一种用于鱼类预防或治疗CyHV-2感染的试剂及应用 |
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