CN109486803B - 工程化苯丙氨酸解氨酶多肽 - Google Patents

工程化苯丙氨酸解氨酶多肽 Download PDF

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Publication number
CN109486803B
CN109486803B CN201811343013.3A CN201811343013A CN109486803B CN 109486803 B CN109486803 B CN 109486803B CN 201811343013 A CN201811343013 A CN 201811343013A CN 109486803 B CN109486803 B CN 109486803B
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pal
amino acid
polypeptide
engineered
seq
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CN109486803A (zh
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吉伽特·W·哈思曼
尼古拉斯·J·阿加德
本杰明·米杰茨
乔纳森·弗罗姆
张希云
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Codexis Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/01—Ammonia-lyases (4.3.1)
    • C12Y403/01005—Phenylalanine ammonia-lyase (4.3.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/01—Ammonia-lyases (4.3.1)
    • C12Y403/01024—Phenylalanine ammonia-lyase (4.3.1.24)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
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  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Neurosurgery (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Neurology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
CN201811343013.3A 2013-04-18 2014-04-17 工程化苯丙氨酸解氨酶多肽 Expired - Fee Related CN109486803B (zh)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201361813586P 2013-04-18 2013-04-18
US61/813,586 2013-04-18
US201361897932P 2013-10-31 2013-10-31
US61/897,932 2013-10-31
PCT/US2014/034500 WO2014172541A2 (en) 2013-04-18 2014-04-17 Engineered phenylalanine ammonia lyase polypeptides
CN201480035201.3A CN105324483B (zh) 2013-04-18 2014-04-17 工程化苯丙氨酸解氨酶多肽

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CN109486803B true CN109486803B (zh) 2022-10-25

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PL2986722T3 (pl) 2013-04-18 2019-10-31 Codexis Inc Skonstruowane polipeptydy amoniakoliazy fenyloalaniny
EP3145536B1 (en) * 2014-04-16 2021-10-27 Codexis, Inc. Engineered tyrosine ammonia lyase
EP3882259B1 (en) 2015-05-13 2026-01-28 Synlogic Operating Company, Inc. Bacteria engineered to reduce hyperphenylalaninemia
CN106636048A (zh) * 2016-12-14 2017-05-10 江南大学 一株酶活提高的苯丙氨酸脱氨酶突变体
CN106755160B (zh) * 2017-01-17 2019-08-02 湖州颐辉生物科技有限公司 一种组氨酸解氨酶及其用途
AU2018217495B2 (en) * 2017-02-13 2021-03-04 Codexis, Inc. Engineered phenylalanine ammonia lyase polypeptides
CN110914288B (zh) * 2017-05-27 2023-05-05 宁波酶赛生物工程有限公司 工程化醛缩酶多肽及其应用
EP3642221A1 (en) 2017-06-21 2020-04-29 Synlogic Operating Company, Inc. Bacteria for the treatment of disorders
EP3807409A4 (en) * 2018-06-12 2022-08-03 Codexis, Inc. Engineered tyrosine ammonia lyase
EP3820833A4 (en) * 2018-07-12 2022-08-03 Codexis, Inc. MODIFIED AMMONIA-LYASE PHENYLALANINE POLYPEPTIDES
JP7780186B2 (ja) * 2018-10-29 2025-12-04 コデクシス， インコーポレイテッド 操作されたｄｎａポリメラーゼバリアント
AU2019397401A1 (en) * 2018-12-14 2021-06-17 Codexis, Inc. Engineered tyrosine ammonia lyase
WO2021168060A1 (en) * 2020-02-19 2021-08-26 Trustees Of Tufts College Methods for engineering amino acid ammonia lyase enzymes and enzymes thereby obtained
WO2021188819A1 (en) * 2020-03-20 2021-09-23 Synlogic Operating Company, Inc. Microorganisms engineered to reduce hyperphenylalaninemia
WO2022146718A1 (en) * 2020-12-31 2022-07-07 Synlogic Operating Company, Inc. Microorganisms engineered to reduce hyperphenylalaninemia
AU2022279241A1 (en) * 2021-05-21 2023-11-30 Syntis Bio, Inc. Engineered methionine gamma lyase variants
CN114015636B (zh) * 2021-12-08 2023-09-29 天地壹号饮料股份有限公司 重组醋酸菌及其制备方法和应用
CN114457151B (zh) * 2022-01-27 2024-06-11 宁夏医科大学总医院 一种用于检测苯丙氨酸羟化酶基因突变的检测试剂盒及其检测方法
CN117417925A (zh) * 2022-07-18 2024-01-19 浙江泽科塔生物医药有限公司 Pal变体、包含该pal变体的药物组合物以及用于制备该pal变体的方法
CN115518691B (zh) * 2022-10-14 2023-11-21 天津大学 一种具有类漆酶活性的人工酶及制备方法和应用
CN115873837A (zh) * 2022-11-18 2023-03-31 安徽盈科生物科技有限公司 高表达的新型的苯丙氨酸解氨酶
CN116478975B (zh) * 2023-06-16 2023-09-01 苏州优信合生技术有限公司 高活性苯丙氨酸解氨酶突变体及其表达菌株
JPWO2025063255A1 (enExample) * 2023-09-19 2025-03-27

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US20190055536A1 (en) 2019-02-21
US10870843B2 (en) 2020-12-22
US10144924B2 (en) 2018-12-04
EP3521423A1 (en) 2019-08-07
JP7129715B2 (ja) 2022-09-02
EP2986722A2 (en) 2016-02-24
MX2015014662A (es) 2016-03-03
US20200032235A1 (en) 2020-01-30
AU2019272057B2 (en) 2021-05-27
US20190055535A1 (en) 2019-02-21
PL2986722T3 (pl) 2019-10-31
JP6637410B2 (ja) 2020-01-29
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EP2986722A4 (en) 2017-02-22
US20200040325A1 (en) 2020-02-06
US10487319B2 (en) 2019-11-26
PE20151775A1 (es) 2015-12-02
AU2014253900A1 (en) 2015-11-12
ES2890955T3 (es) 2022-01-25
CN105324483B (zh) 2018-11-30
US20200399627A1 (en) 2020-12-24
JP2020185016A (ja) 2020-11-19
CN105324483A (zh) 2016-02-10
US20140314843A1 (en) 2014-10-23
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US11136571B2 (en) 2021-10-05
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US9611468B2 (en) 2017-04-04
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US20190241882A1 (en) 2019-08-08
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US10975367B2 (en) 2021-04-13
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US20180016568A1 (en) 2018-01-18
US11401515B2 (en) 2022-08-02
US10640759B2 (en) 2020-05-05
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US20200032236A1 (en) 2020-01-30
PT2986722T (pt) 2019-06-21
JP2016516428A (ja) 2016-06-09
EP3521423B1 (en) 2021-07-21
CN109486803A (zh) 2019-03-19
JP2018166524A (ja) 2018-11-01
US11913045B2 (en) 2024-02-27
EA034034B1 (ru) 2019-12-20
MY193198A (en) 2022-09-26
AU2014253900B2 (en) 2019-09-19
US10717974B2 (en) 2020-07-21
US20200032233A1 (en) 2020-01-30
JP2020185017A (ja) 2020-11-19

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