CN109415735A - 高效表达重组人血清白蛋白工程菌的构建 - Google Patents
高效表达重组人血清白蛋白工程菌的构建 Download PDFInfo
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Abstract
本发明提供了一种高效表达重组人血清白蛋白的方法,其特征在于包括以下步骤:在酵母宿主细胞中,将(a)人血清白蛋白基因与(b)一种或多种rHSA表达促进因子基因进行共表达。
Description
PCT国内申请,说明书已公开。
Claims (14)
- PCT国内申请,权利要求书已公开。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202011139443.0A CN112831501A (zh) | 2016-05-04 | 2017-05-04 | 高效表达重组人血清白蛋白工程菌的构建 |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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CN201610289898 | 2016-05-04 | ||
CN2016102898988 | 2016-05-04 | ||
PCT/CN2017/083079 WO2017190671A1 (zh) | 2016-05-04 | 2017-05-04 | 高效表达重组人血清白蛋白工程菌的构建 |
Related Child Applications (1)
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CN202011139443.0A Division CN112831501A (zh) | 2016-05-04 | 2017-05-04 | 高效表达重组人血清白蛋白工程菌的构建 |
Publications (2)
Publication Number | Publication Date |
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CN109415735A true CN109415735A (zh) | 2019-03-01 |
CN109415735B CN109415735B (zh) | 2020-11-13 |
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CN201780027907.9A Active CN109415735B (zh) | 2016-05-04 | 2017-05-04 | 高效表达重组人血清白蛋白工程菌的构建 |
CN202011139443.0A Pending CN112831501A (zh) | 2016-05-04 | 2017-05-04 | 高效表达重组人血清白蛋白工程菌的构建 |
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Country Status (8)
Country | Link |
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US (1) | US20190330707A1 (zh) |
EP (1) | EP3453763B1 (zh) |
JP (2) | JP7014774B2 (zh) |
CN (2) | CN109415735B (zh) |
AU (1) | AU2017259656B2 (zh) |
CA (1) | CA3023046A1 (zh) |
IL (1) | IL262752B (zh) |
WO (1) | WO2017190671A1 (zh) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20190330707A1 (en) | 2016-05-04 | 2019-10-31 | Shenzhen Protgen Ltd. | Construction of engineering bacteria for high expression of recombinant human serum albumin |
CN109988803A (zh) * | 2019-05-16 | 2019-07-09 | 齐智 | 一种高效生产重组人血清白蛋白的发酵方法 |
CN111363688A (zh) * | 2020-03-16 | 2020-07-03 | 通化安睿特生物制药股份有限公司 | 一种提高重组人白蛋白表达质量减少降解的方法和用途 |
CN112143662B (zh) * | 2020-08-31 | 2023-01-06 | 浙江工业大学 | 一种重组毕赤酵母工程菌及在制备嘌呤中的应用 |
CN112898407B (zh) * | 2021-01-25 | 2022-07-12 | 南京医科大学 | 重组驼源血清白蛋白的制备方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102643847A (zh) * | 2011-02-17 | 2012-08-22 | 华东理工大学 | 多基因共表达体系及含二硫键功能性蛋白的生产方法 |
CN102766648A (zh) * | 2012-07-25 | 2012-11-07 | 浙江大学 | 人血清白蛋白与白介素1受体拮抗剂融合蛋白的表达系统 |
CN104152484A (zh) * | 2014-08-13 | 2014-11-19 | 青岛蔚蓝生物集团有限公司 | 一种提高毕赤酵母分泌型外源蛋白表达量的方法 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0638771A (ja) * | 1990-10-31 | 1994-02-15 | Tonen Corp | ヒトプロテインジスルフィドイソメラーゼ遺伝子の発現方法および該遺伝子との共発現によるポリペプチドの製造方法 |
EP0509841A3 (en) * | 1991-04-18 | 1993-08-18 | Tonen Corporation | Co-expression system of protein disulfide isomerase gene and useful polypeptide gene and process for producing the polypeptide using its system |
JPH06189769A (ja) * | 1992-10-30 | 1994-07-12 | Green Cross Corp:The | 変異型aox2プロモーター、それを担持するベクター、形質転換体および異種蛋白質の製造方法 |
US5521086A (en) * | 1993-09-16 | 1996-05-28 | Cephalon, Inc. | Secretion sequence for the production of a heterologous protein in yeast |
GB0329681D0 (en) * | 2003-12-23 | 2004-01-28 | Delta Biotechnology Ltd | Gene expression technique |
CN101665798B (zh) * | 2008-09-06 | 2012-11-21 | 浙江我武生物科技股份有限公司 | 一种制备重组人血清白蛋白与干扰素α融合蛋白的方法 |
EP2565262A1 (en) * | 2011-08-31 | 2013-03-06 | VTU Holding GmbH | Protein expression |
SG11201700665YA (en) * | 2014-07-30 | 2017-02-27 | Daiichi Sankyo Co Ltd | Method for improved high-level secretory production of protein |
US20190330707A1 (en) | 2016-05-04 | 2019-10-31 | Shenzhen Protgen Ltd. | Construction of engineering bacteria for high expression of recombinant human serum albumin |
-
2017
- 2017-05-04 US US16/099,159 patent/US20190330707A1/en active Pending
- 2017-05-04 CN CN201780027907.9A patent/CN109415735B/zh active Active
- 2017-05-04 WO PCT/CN2017/083079 patent/WO2017190671A1/zh unknown
- 2017-05-04 JP JP2019510736A patent/JP7014774B2/ja active Active
- 2017-05-04 CN CN202011139443.0A patent/CN112831501A/zh active Pending
- 2017-05-04 CA CA3023046A patent/CA3023046A1/en active Pending
- 2017-05-04 EP EP17792497.4A patent/EP3453763B1/en active Active
- 2017-05-04 AU AU2017259656A patent/AU2017259656B2/en active Active
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2018
- 2018-11-04 IL IL262752A patent/IL262752B/en unknown
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2022
- 2022-01-17 JP JP2022005006A patent/JP7448977B2/ja active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102643847A (zh) * | 2011-02-17 | 2012-08-22 | 华东理工大学 | 多基因共表达体系及含二硫键功能性蛋白的生产方法 |
CN102766648A (zh) * | 2012-07-25 | 2012-11-07 | 浙江大学 | 人血清白蛋白与白介素1受体拮抗剂融合蛋白的表达系统 |
CN104152484A (zh) * | 2014-08-13 | 2014-11-19 | 青岛蔚蓝生物集团有限公司 | 一种提高毕赤酵母分泌型外源蛋白表达量的方法 |
Non-Patent Citations (1)
Title |
---|
TIZIANA LODI ET AL.: "Secretion of Human Serum Albumin by Kluyveromyces lactis Overexpressing KlPDI1 and KlERO1", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 * |
Also Published As
Publication number | Publication date |
---|---|
CN109415735B (zh) | 2020-11-13 |
EP3453763A4 (en) | 2019-11-27 |
EP3453763A1 (en) | 2019-03-13 |
CN112831501A (zh) | 2021-05-25 |
IL262752B (en) | 2022-01-01 |
IL262752A (en) | 2018-12-31 |
JP2019516401A (ja) | 2019-06-20 |
AU2017259656B2 (en) | 2023-01-12 |
CA3023046A1 (en) | 2017-11-09 |
JP2022043355A (ja) | 2022-03-15 |
AU2017259656A1 (en) | 2018-12-20 |
EP3453763B1 (en) | 2021-12-22 |
WO2017190671A1 (zh) | 2017-11-09 |
JP7014774B2 (ja) | 2022-02-01 |
JP7448977B2 (ja) | 2024-03-13 |
US20190330707A1 (en) | 2019-10-31 |
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