CN109315225A - A kind of gastrodin cultivation bacteria stick and preparation method thereof - Google Patents
A kind of gastrodin cultivation bacteria stick and preparation method thereof Download PDFInfo
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- CN109315225A CN109315225A CN201811245570.1A CN201811245570A CN109315225A CN 109315225 A CN109315225 A CN 109315225A CN 201811245570 A CN201811245570 A CN 201811245570A CN 109315225 A CN109315225 A CN 109315225A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Cultivation Of Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to technical field of cultivation of gastrodia elata, more particularly to a kind of gastrodin cultivation bacteria stick and preparation method thereof, present invention bacteria stick includes following material: branch of poplar, mulberry tree branch, pine needle, sodium alginate, shaddock leaf, thin rice gruel, corn soybean milk, peanut shell, peach-pit, the present invention also provides the preparation methods of the bacteria stick, bacteria stick low manufacture cost of the invention, without miscellaneous bacteria, insect pest and virus, and bacterium germination speed is fast, nutrient absorption utilization rate is high, conventional cultivation is carried out using bacteria stick of the invention, not only increase the active constituent content of Rhizoma Gastrodiae, drought resisting disease resistance, also shorten cultivation period.
Description
Technical field
The present invention relates to technical field of cultivation of gastrodia elata, and in particular to a kind of gastrodin cultivation bacteria stick and preparation method thereof.
Background technique
Rhizoma Gastrodiae is the orchid of a kind of unrooted, redgreen leaf, and Wild gastrodia is by thinking to guess at random the destructive shadow disorderly dug
Ring, resource is extremely limited, cannot absorb and manufacture nutrition, it is necessary to by with halimasch symbiosis could growth and development, this makes
Bacterium material becomes essential substance in gastrodin cultivation.
If patent No. CN201810054115.7 discloses a kind of bacteria stick and preparation method thereof for gastrodin cultivation, it is related to
Technical field of cultivation of gastrodia elata, bacteria stick of the present invention using bamboo as stick core, bamboo outer layer wrap respectively straw, sawdust or/and
Plant growth substance, empty in bamboo, outer layer straw has more gap, can effectively ensure that halimasch oxygen throughput；Straw and
Sawdust and Rice & peanut milk paste outside straw can provide sufficient glycogen and nutriment for the growth of halimasch, for mycelia and numb kind of life
It is long.Bacteria stick is made using discarded sawdust or branch in the invention, protects broadleaf tree to be cut down without limit, protects gloomy
Woods resource and environment；It solves the contradiction between Rhizoma Gastrodiae popularizing planting and ecological environmental protection, provides one for the plantation of Rhizoma Gastrodiae
A new approach, suitable for Rhizoma Gastrodiae plantation popularization and expansion, can be formed the planting scale of modernization, industrialization and standardization with
Meet demand.
For another example patent No. CN201510191984.0 discloses a kind of a kind of culture medium of halimasch parent species of offer, this hair
A kind of method using above-mentioned culture medium culture Armillaria mellea of bright the disclosed, which, which also discloses a kind of cotton stalk, makes
With the method for above-mentioned Armillaria mellea production bacteria stick, includes the following steps: cotton stalk pack sterilizing, then take liquid halimasch
4~6ml of strain is seeded on cotton stalk, and inoculation temperature is 20~22 DEG C；Cotton stalk after inoculation controlled at 20~
25 DEG C, moisture content keeps 40-50%, and growth time is bacteria stick to be made in 35~45 days, which also discloses a kind of cotton stalk
The method of the bacteria stick cultivation Rhizoma Gastrodiae of production guarantees the big low altitude area day Taro Aso of the four seasons temperature difference using the technical solution of the invention
Long stabilization, the method for the bacteria stick cultivation Rhizoma Gastrodiae of cotton stalk production, has found for a large amount of cotton stalk and plays its economic valence
The outlet of value solves and burns the environmental pollution of cotton stalk bring.
The prior art is not significant for the conversion absorptivity effect of effective component, so that effective component is lost, waste.
Summary of the invention
The present invention is in order to solve the above technical problems, the present invention provides a kind of gastrodin cultivation bacteria sticks and preparation method thereof.
Specifically realized according to following technical scheme:
The present invention provides a kind of gastrodin cultivation bacteria sticks, by weight include following material: 32-38 parts of branch of poplar,
Mulberry tree branch 25-37 parts, 21-29 parts of pine needle, 0.7-1.6 parts of sodium alginate, 8-15 parts of shaddock leaf, 16-22 parts of thin rice gruel, corn soybean milk
2-9 parts, 13-29 parts of peanut shell, 17-31 parts of peach-pit.
It further, by weight include following material: 34 parts of branch of poplar, mulberry tree branch 31 parts, 24 parts of pine needle, alginic acid
1.1 parts of sodium, 12 parts of shaddock leaf, 19 parts of thin rice gruel, 5 parts of corn soybean milk, 21 parts of peanut shell, 23 parts of peach-pit.
It according to solid-to-liquid ratio is 1:(7-10 that the thin rice gruel, which is by rice, water) ratio in temperature be that 75-85 DEG C of condition is lauched
Boil 1.5-2h.
It is 1:(4-5 that the corn soybean milk is by corn, soya bean by weight) ratio mixing after, adding water to solid-to-liquid ratio is
Ratio 1:(17-20) is stirred under the conditions of temperature is 85-90 DEG C to 60-80 mesh excessively.
The present invention also provides a kind of preparation methods of gastrodin cultivation bacteria stick, include the following steps:
S1 energization processing: shaddock leaf, peanut shell, peach-pit were crushed to 50-60 mesh, pulverising step resulting material was set
After the processing 60-90s that is powered in container, it is spare to obtain mixture A；
S2 ultrasonication: corn soybean milk, thin rice gruel, sodium alginate are placed in ultrasonication in ultrasonic response container
It is spare to obtain mixture B by 20-30s；
The processing of S3 one time fermentation: it after mixture A, mixture B are sufficiently mixed, ferments under the conditions of being 20-33 DEG C in temperature
3-5 days, be powered processing 10-20s, obtains fermentation material A；
The processing of S4 secondary fermentation: being crushed to 50-60 mesh for branch of poplar, mulberry tree branch, spare；Then by fermentation material A with
Branch of poplar, mulberry tree branch, the pine needle of crushing are uniformly mixed, and are fermented 2-7 days under the conditions of being 15-23 DEG C in temperature, are obtained bacteria stick.
Be powered in the energization processing step processing voltage be 110-120V.
Ultrasonic frequency is 36-58KHz in the sonicating step.
Be powered in the one time fermentation processing step processing voltage be 160-180V.
The a length of 10-30cm of bacteria stick, diameter 8-15cm.
The beneficial effects of the present invention are:
Bacteria stick low manufacture cost of the invention is free of miscellaneous bacteria, insect pest and virus, and bacterium germination speed is fast, and nutrient absorption utilizes
Rate is high, carries out conventional cultivation using bacteria stick of the invention, not only increases active constituent content, the drought resisting disease resistance of Rhizoma Gastrodiae,
Also shorten cultivation period；Cultivating or so July can be harvested, and conversion ratio reaches 80.8% or more, and gastrodin content reaches
Reach 50.3% or more to 1.5% or more, gastrodia elata polysaccharide content.
Shaddock leaf, peanut shell, peach-pit, branch of poplar, mulberry tree branch are crushed to 50-60 mesh by the present invention, and bacterium has been effectively ensured
The porosity and oxygen-supply quantity of stick, aforementioned substances are cheap and easy to get, are the desirable feedstocks of microorganism good nutriment and compost, but
It is that this raw material has low containing miscellaneous bacteria and nutrient utilization, generally requires sterilized and effective component extraction process,
And the present invention uses the power on processing, on the one hand improve miscellaneous bacteria there are situations, on the other hand improve the materialization of effective component
Activity.
Corn soybean milk, thin rice gruel, sodium alginate are ultrasonically treated by the present invention, improve protein, polysaccharide etc. effectively at
The activity divided, while improving the adhesive property, viscous property and space structure of clayey ingredient.
Product after ultrasonic treatment and treated the product of being powered are subjected to fermentation process, on the one hand further leached effective
On the other hand ingredient inhibits varied bacteria growing, and may insure effective component and enzyme non-inactivation, and benefit using Sonicates
With nutritional status to fermentation material is changed in thin rice gruel, corn soybean milk, so that probiotics annexes corrupt strain, so that prebiotic
Strain forms superiority, and collaboration increases；It adds branch of poplar, mulberry tree to ferment, facilitates the growth of halimasch.
The present invention controls the combination of the space structures such as granularity and the sodium alginate of raw material, effectively controls oxygen-supply quantity, adjusts
Mycelia growth balance, and then have adjusted the growth balance of Rhizoma Gastrodiae.
Specific embodiment
Specific embodiments of the present invention will be described in further detail below, but the invention is not limited to these realities
Mode is applied, it is claimed to still fall within the claims in the present invention for any improvement or replacement on the present embodiment essence spirit
Range.
Embodiment 1
Present embodiments provide a kind of gastrodin cultivation bacteria stick, including following material: branch of poplar 32kg, mulberry tree branch 25kg,
Pine needle 21kg, sodium alginate 0.7kg, shaddock leaf 8kg, thin rice gruel 16kg, corn soybean milk 2kg, peanut shell 13kg, peach-pit 17kg；
The thin rice gruel be by rice, water be 1:7 according to solid-to-liquid ratio ratio under the conditions of temperature is 75 DEG C boiling 1.5h；
The corn soybean milk is by corn, soya bean be by weight 1:4 ratio mixing after, add water to solid-to-liquid ratio be 1:17
Ratio, temperature be 85 DEG C under the conditions of stirring to cross 60 meshes.
Embodiment 2
Present embodiments provide a kind of gastrodin cultivation bacteria stick, including following material: branch of poplar 38kg, mulberry tree branch 37kg,
Pine needle 29kg, sodium alginate 1.6kg, shaddock leaf 15kg, thin rice gruel 22kg, corn soybean milk 9kg, peanut shell 29kg, peach-pit 31kg；
The thin rice gruel be by rice, water be 1:10 according to solid-to-liquid ratio ratio under the conditions of temperature is 85 DEG C boiling 2h；
The corn soybean milk is by corn, soya bean be by weight 1:5 ratio mixing after, add water to solid-to-liquid ratio be 1:20
Ratio, temperature be 90 DEG C under the conditions of stirring to cross 80 meshes.
Embodiment 3
Present embodiments provide a kind of gastrodin cultivation bacteria stick, including following material: branch of poplar 35kg, mulberry tree branch 35kg,
Pine needle 23kg, sodium alginate 1kg, shaddock leaf 11kg, thin rice gruel 17kg, corn soybean milk 8kg, peanut shell 15kg, peach-pit 30kg；
The thin rice gruel be by rice, water be 1:9 according to solid-to-liquid ratio ratio under the conditions of temperature is 82 DEG C boiling 1.5h；
The corn soybean milk is by corn, soya bean be by weight 1:4.8 ratio mixing after, add water to solid-to-liquid ratio be 1:
18 ratio is stirred under the conditions of temperature is 88 DEG C to 65 meshes excessively.
Embodiment 4
Present embodiments provide a kind of gastrodin cultivation bacteria stick, including following material: branch of poplar 34kg, mulberry tree branch 31kg,
Pine needle 24kg, sodium alginate 1.1kg, shaddock leaf 12kg, thin rice gruel 19kg, corn soybean milk 5kg, peanut shell 21kg, peach-pit 23kg；
The thin rice gruel be by rice, water be 1:8 according to solid-to-liquid ratio ratio under the conditions of temperature is 80 DEG C boiling 2h；
The corn soybean milk is by corn, soya bean be by weight 1:4.5 ratio mixing after, add water to solid-to-liquid ratio be 1:
19 ratio is stirred under the conditions of temperature is 87 DEG C to 70 meshes excessively.
Embodiment 5
The present embodiment provides a kind of preparation method of gastrodin cultivation bacteria stick on the basis of embodiment 1-4 is any, including
Following steps:
S1 energization processing: shaddock leaf, peanut shell, peach-pit were crushed to 50-60 mesh, pulverising step resulting material was set
In in container, it is powered after processing 60-90s under conditions of voltage is 110-120V, it is spare obtains mixture A；
S2 ultrasonication: corn soybean milk, thin rice gruel, sodium alginate are placed in ultrasonic response container, in 36-58KHz
Under conditions of ultrasonication 20-30s, it is spare to obtain mixture B；
The processing of S3 one time fermentation: it after mixture A, mixture B are sufficiently mixed, ferments under the conditions of being 20-33 DEG C in temperature
3-5 days, be powered processing 10-20s under conditions of voltage is 160-180V, obtains fermentation material A；
The processing of S4 secondary fermentation: being crushed to 50-60 mesh for branch of poplar, mulberry tree branch, spare；Then by fermentation material A with
Branch of poplar, mulberry tree branch, the pine needle of crushing are uniformly mixed, and are fermented 2-7 days under the conditions of being 15-23 DEG C in temperature, are obtained bacteria stick, wherein bacterium
Stick a length of 10-30cm, diameter 8-15cm.
Comparative example 1
Using straw 1000kg, sawdust 200kg, calcium superphosphate 100kg and potassium dihydrogen phosphate 18kg, calcium sulfate, sulfuric acid
Magnesium and the mixture 35kg of soluble silica are as compost culture Rhizoma Gastrodiae.
Comparative example 2
Present embodiments provide a kind of gastrodin cultivation bacteria stick, including following material: branch of poplar 35kg, mulberry tree branch 35kg,
Pine needle 23kg, shaddock leaf 11kg, thin rice gruel 17kg, corn soybean milk 8kg, peanut shell 15kg, peach-pit 30kg；
The thin rice gruel be by rice, water be 1:9 according to solid-to-liquid ratio ratio under the conditions of temperature is 82 DEG C boiling 1.5h；
The corn soybean milk is by corn, soya bean be by weight 1:4.8 ratio mixing after, add water to solid-to-liquid ratio be 1:
18 ratio is stirred under the conditions of temperature is 88 DEG C to 65 meshes excessively.
Effect example
Using embodiment 3, comparative example 1, the bacteria stick of comparative example 2 and traditional bacteria stick, identical method and growth ring are utilized
Border is colonized 16 hoary hair fiber crops according to every pool, has been colonized 20 pools altogether and has been cultivated, and statistics Rhizoma Gastrodiae growing state is as shown in table 1；
Table 1
| Crop cycle (moon) | At numb rate (%) | Arrow fiber crops yield (pool g/) | Hoary hair fiber crops yield (pool g/) | |
| Embodiment 1 | 7 | 98.2 | 1493.26 | 164.04 |
| Comparative example 1 | 9 | 95.1 | 1471.44 | 161.35 |
| Comparative example 2 | 8.5 | 93.6 | 1465.66 | 162.18 |
| Traditional bacteria stick | 12.5 | 89.9 | 1446.92 | 160.60 |
Claims (9)
1. a kind of gastrodin cultivation bacteria stick, which is characterized in that by weight include following material: 32-38 parts of branch of poplar, mulberry tree
Branch 25-37 parts, 21-29 parts of pine needle, 0.7-1.6 parts of sodium alginate, 8-15 parts of shaddock leaf, 16-22 parts of thin rice gruel, corn soybean milk 2-9
Part, 13-29 parts of peanut shell, 17-31 parts of peach-pit.
2. gastrodin cultivation bacteria stick as described in claim 1, which is characterized in that by weight include following material: poplar
34 parts of branch, mulberry tree branch 31 parts, 24 parts of pine needle, 1.1 parts of sodium alginate, 12 parts of shaddock leaf, 19 parts of thin rice gruel, 5 parts of corn soybean milk, peanut
21 parts of shell, 23 parts of peach-pit.
3. gastrodin cultivation bacteria stick as described in claim 1, which is characterized in that the thin rice gruel is by rice, water according to solid-liquid
Than the ratio for 1:(7-10) under the conditions of temperature is 75-85 DEG C boiling 1.5-2h.
4. gastrodin cultivation bacteria stick as described in claim 1, which is characterized in that the corn soybean milk be by corn, soya bean by
Weight ratio is 1:(4-5) ratio mixing after, add water to solid-to-liquid ratio be 1:(17-20) ratio, temperature be 85-90 DEG C of condition
It is lower to stir to 60-80 mesh excessively.
5. the preparation method of gastrodin cultivation bacteria stick as described in claim 1, which comprises the steps of:
S1 energization processing: shaddock leaf, peanut shell, peach-pit were crushed to 50-60 mesh, pulverising step resulting material is placed in appearance
It is powered after processing 60-90s in device, it is spare to obtain mixture A；
S2 ultrasonication: corn soybean milk, thin rice gruel, sodium alginate are placed in ultrasonication 20- in ultrasonic response container
It is spare to obtain mixture B by 30s；
The processing of S3 one time fermentation: after mixture A, mixture B are sufficiently mixed, ferment 3-5 under the conditions of being 20-33 DEG C in temperature
It, be powered processing 10-20s, obtains fermentation material A；
The processing of S4 secondary fermentation: being crushed to 50-60 mesh for branch of poplar, mulberry tree branch, spare；Then by fermentation material A and crushing
Branch of poplar, mulberry tree branch, pine needle be uniformly mixed, in temperature be 15-23 DEG C under the conditions of ferment 2-7 days, obtain bacteria stick.
6. the preparation method of gastrodin cultivation bacteria stick as described in claim 1, which is characterized in that in the energization processing step
The voltage handled that is powered is 110-120V.
7. the preparation method of gastrodin cultivation bacteria stick as described in claim 1, which is characterized in that the sonicating step
Middle ultrasonic frequency is 36-58KHz.
8. the preparation method of gastrodin cultivation bacteria stick as described in claim 1, which is characterized in that the one time fermentation processing step
The voltage handled that is powered in rapid is 160-180V.
9. the preparation method of gastrodin cultivation bacteria stick as described in claim 1, which is characterized in that a length of 10- of bacteria stick
30cm, diameter 8-15cm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117337723A (en) * | 2023-11-24 | 2024-01-05 | 西南林业大学 | Gastrodia elata cultivation medium and preparation method and application thereof |
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