CN109302932A - A kind of cultural method keeping kwangsi mayten herb characteristic - Google Patents
A kind of cultural method keeping kwangsi mayten herb characteristic Download PDFInfo
- Publication number
- CN109302932A CN109302932A CN201811161340.7A CN201811161340A CN109302932A CN 109302932 A CN109302932 A CN 109302932A CN 201811161340 A CN201811161340 A CN 201811161340A CN 109302932 A CN109302932 A CN 109302932A
- Authority
- CN
- China
- Prior art keywords
- parts
- culture substrate
- powder
- water
- seed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/17—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing slag
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/24—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients to enhance the sticking of the active ingredients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Soil Sciences (AREA)
- Toxicology (AREA)
- Environmental & Geological Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Plant Substances (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to planting technology fields, specifically disclose a kind of cultural method for keeping kwangsi mayten herb characteristic, the specific steps are as follows: the first step, the preparation of culture substrate；Second step, seed preparation；Third step, sprouting processing；4th step, nursery is cultivated out.The invention has the advantage, that the cultural method by the application, it can be improved the germination percentage of seed, and the activity of the effective component contained in the kwangsi mayten herb that the activity for the effective component for improving outplanting rate, and containing in kwangsi mayten herb is obtained compared to tissue cultures is higher.
Description
[technical field]
The invention belongs to planting technology fields, and in particular to a kind of cultural method for keeping kwangsi mayten herb characteristic.
[background technique]
Kwangsi mayten herb (MaytenusguangxiensisC.Y.ChengetW.L.Sha) belongs to normal for Celastraceae Caulis Mayteni
Green shrub is Karst Region of Guangxi peculiar medicinal plants, and the Limestone Mountain for being distributed in the ground such as big new, Chongzuo, Guangxi, Pingxiang, Ningming fills
Cong Zhong.The root of kwangsi mayten herb, stem contain anticancer active constituent maytenin (Maytansine) and Mei Dengbulin in leaf
(Maytanprrine), have to malignant tumours such as breast cancer, digestive system cancer, lymph meat cancer, bone marrow cancer, chronic myelocytic leukemias
Significant curative effect.
The production of kwangsi mayten herb deteriorates due to living environment mainly by wild resource and the producing region masses disorderly adopts excessively for many years
It cuts down, wild resource reserves are fewer and fewer, Critical Condition are in, by " SOUTHERN CHINA and west and south limestone Precious, Rare, Endangered are planted
Name record " it is classified as rare extinction plants.
Most portion of tissue with kwangsi mayten herb carry out tissue cultures in the prior art, but pass through multiple tissue and train
Support after grow into tree after, tissue in active constituent, substantially reduce, for example, be equally one formula Chinese herbal medicine carry out bubble medicine
Wine, the herbal medicine efficacy effect of large quantities of large-scale productions be exactly it is good not as wild, therefore, it is necessary to carry out to kwangsi mayten herb
Seminal propagation, but the kwangsi mayten herb emergence rate of seminal propagation is low with outplanting rate, therefore being badly in need of finding one kind can be effective
Improve the method that kwangsi mayten herb seminal propagation improves emergence rate and outplanting rate.
[summary of the invention]
The object of the present invention is to provide a kind of cultural methods for keeping kwangsi mayten herb characteristic, pass through the cultivation side of the application
The activity of method, the effective component for can be improved the germination percentage of seed, and improving outplanting rate, and containing in kwangsi mayten herb is compared
The activity of the effective component contained in the kwangsi mayten herb that tissue cultures obtain is higher.
To achieve the goals above, The technical solution adopted by the invention is as follows:
A kind of cultural method keeping kwangsi mayten herb characteristic, the specific steps are as follows:
The first step, the preparation of culture substrate: culture substrate in parts by weight, is prepared: red soil by following raw material
10-15 parts native, 5-10 parts of coal ash, sand 8-12 parts middle, 10-15 parts of mushroom slag, 5-8 parts of sawdust, 5-10 parts of sugarcane top, 5-8 parts of chicken manure,
5-8 parts of rice washing water, bamboo silk 7-10 parts, 0.5-1.5 parts of EM bacterium, 1.5-2.5 parts of pomegranate leaf powder, 2.5-3.5 parts of leaf of Moringa powder, Phytolacca acinosa
1.5-3.5 parts of root powder；
Seed preparation: second step the full kwangsi mayten herb seed of grain is washed with clean water, soak is then immersed in
It is 10-15 minutes middle, it then pulls out, placement is dried at normal temperature, the use of power is then 250-300W, frequency is 200-300 conspicuous
Ultrasonication hereby 0.5-1.5 minutes is then that 2000-2500lx irradiates 12-15S, the behaviour of irradiation using ultraviolet ray intensity
Make mode are as follows: irradiation 3S- stops irradiation 10S, and then seed is placed at 32 DEG C and keeps the temperature preservation；
The soak is mixed to get by following raw material: water 100ml, bananas juice 20-25ml, bean sprout juice 15-20ml, kind
Tomato juice 10-20ml, rubber powder 1.5-3.5g；
Sprouting processing: culture substrate after culture substrate is carried out high-temperature sterilization, is laid in seedling bed immediately by third step
On, the thickness of culture substrate tiling is 15 centimetres, is 10 centimetres according to line-spacing for culture substrate and starts, and capable depth is 3-
5 centimetres, the temperature at detection culture substrate center reaches 30 DEG C, immediately by seed sowing in the row opened up, and between every seed
Every 10-15 centimetres, culture substrate is then backfilled, at being 25 DEG C in temperature, intensity of illumination 2000-2500lx, light application time is
12 hours daily, after sowing 3 days, starts watering to keep the humidity of culture substrate to be 30-40%, water is opened after sowing 10-12 days
Beginning pours promoting root growth liquid instead to keep culture substrate humidity for 30-40%, the germination until seed breaks ground；
The promoting root growth liquid is mixed to get by following raw material: basic element of cell division 5-8ml, gibberellin 3-5ml, auxin 8-
13ml, fleabane flower extracting solution 10-15ml, jasmonic 2.5-5.5ml, molasses 3-5g/ml, water 50-70ml；4th step, out nursery
Culture: after germination after long to 5-10 centimetres of seedling, start to seedling liquid fertilizer sprayed, 10-20Kg/ mus, other fields
Management is normally carried out, weeding, insect prevention, until nursery out.
Further, the culture substrate is prepared in accordance with the following methods:
(1) sugarcane top is crushed and sodium carboxymethylcellulose mixing is added, accumulation processing 36-58 hours, carboxymethyl cellulose
The additional amount of sodium is the 5-8% of sugarcane top weight；
(2) chicken manure is sterilized using ultraviolet light, exposure intensity 2500-3000lx, irradiation is turned over primary after ten minutes
Chicken manure continues irradiation 10 minutes, repeatedly 3-5 times；
(3) by red soil, coal ash, middle sand, mushroom slag, sawdust, treated sugarcane top, treated chicken manure, rice washing water, bamboo
Heap fermentation is built in mixing after silk, EM bacterium weigh according to parts by weight, and after fermentation 20-30 days, to fermentation material progress, stirring is subsequent continues heap
Fermentation process 5-20 days, and keep the fermentation material relative humidity for building heap in 30-40%, premix culture substrate can be obtained；
(4) boiling 10-20 points will be added in high-temperature steam cooker after picking pomegranate leaf in time, then takes out and is ground while hot
Slurry is worn into, ethanol solution is added and carries out water-bath refluxing extraction 30-50 minutes, takes filtrate, filter residue continues cycling through refluxing extraction 3-5
Secondary, then merging filtrate is spray-dried to obtain pomegranate leaf powder；
(5) it is dried after cleaning leaf of Moringa, 10 meshes is crossed after grinding, obtain leaf of Moringa powder；
(6) its root is taken after picking Phytolacca acinosa, is sliced, is dried naturally, powder is then polished into, and pokeberry root can be obtained
Powder；
(7) culture medium is can be obtained into premix culture substrate and pomegranate leaf powder, leaf of Moringa powder, pokeberry root powder after mixing
Matter.
Further, in step (4), ethanol solution and the volume ratio for grinding the pomegranate leaf being slurried are 5-8:1；Ethyl alcohol is molten
The mass concentration of liquid is 75%；The temperature of water-bath is 40 DEG C.
Further, in step (3), EM bacterium also first passes through activation processing.
Further, the temperature of the activation processing is 30-32 DEG C.
Further, the concrete operation step of the activation processing is: banana puree 5g, sweet potato mash 3g are mixed with EM bacterium and stirred
It mixes and uniformly obtains mixed soil, after placing 1 minute, take conical flask that suitable quantity of water is added to add peptone 3g, beef extract 5g heating water bath
Constant temperature is completely dissolved to being stirred continuously after 40 DEG C to peptone, beef extract, after it is naturally cool be cooled to 35 DEG C after be added banana polysaccharide 2g,
Polysaccharides in Bamboo Leaves 1.5g, sodium chloride 3.5g and water are settled to 500ml, are then that 30-40rpm/min shaking flask activation 8-10 is small in revolution
When.
It further illustrates, banana puree is that the androgynous ponding of slice addition stirs into mud after taking banana to remove the peel.
It further illustrates, sweet potato mash is that androgynous ponding is added after being sliced after taking sweet potato to remove the peel to stir into mud.
It further illustrates, the banana polysaccharide is prepared by following scheme: being sliced, dry after banana is removed the peel, powder
It is broken, enter in conical flask while conical flask is added in dehydrated potato powder from conical flask entrance shower water, banaina and water are according to weight
Than carrying out boiling water bath boiling 20-30 points then for 1:5, then three layers of filtered through gauze take filtrate, and filtrate is then used centrifuge
It is centrifuged, 3000-3500rpm is centrifuged 20-30S, takes supernatant, then carries out the concentrate for being concentrated into 30 ° of mother-in-law's U.S. degree, then
By mass fraction be 75% ethanol solution pour into side bevelling stirring, then generate a large amount of flocculent deposit, then temperature be-
2 DEG C to -3 DEG C freezing 5-8 hours, then reducing pressure is 140-155 pa, and temperature can be obtained dry 5-6 hours at 28 DEG C
Banana polysaccharide.Using dehydrated potato powder is added simultaneously and water can be improved the efficiency and recovery rate of extraction, recovery rate improves 0.5-
0.8%.
It further illustrates, the Polysaccharides in Bamboo Leaves is prepared by following scheme: by leaf of bamboo clean dry, crushing, then
Bamboo leaf powder is added to the distilled water of 5 times of volumes, is entered while conical flask is added in bamboo leaf powder from conical flask entrance spray distilled water water
It in conical flask, and is handled 3-5 minutes in the case where power is 250W microwave, then starts boiling 25-35 minutes, filter to take filtrate, subtract
Pressure is concentrated into 1.11 times of original volume, then temperature be -2 DEG C to -3 DEG C freezing 3-4 hours, be then centrifuged for machine 3000-
3500rpm is centrifuged 5-8 minutes, takes supernatant, and dehydrated alcohol is then added and carries out precipitating 2-3 times, is taken by ultrafiltration membrance filter
Precipitating obtains Polysaccharides in Bamboo Leaves after precipitating vacuum freeze-drying.Using be added bamboo leaf powder simultaneously and water can be improved extraction efficiency and
Recovery rate, recovery rate improve 0.3-0.6%.
In conclusion by adopting the above-described technical solution, the beneficial effects of the present invention are:
The application seed is impregnated by soak, can be improved the germination percentage of plantation.Wherein bananas juice is that banana is added 2
It carries out beating juice after the water of times volume and crosses filter residue, contain protein, sugar, potassium, vitamin A and C in bananas juice；Bean sprout juice is by soya bean
Dozen juice is carried out after the water of bud 2 times of volumes of addition and crosses filter residue, contains protein, vitamin C, carbohydrate, isodynamic enzyme etc. in bean sprout juice；Kind
Tomato, which is added after the water of 2 times of volumes, to carry out beating juice for tomato juice crosses filter residue, and tomatin, sugar, vitamin A, B.C, D are contained in tomato juice
And organic acid and enzyme etc., after bananas juice, bean sprout juice, tomato juice compounding will there is various trace elements, a great number of elements and enzyme to have
There is the effect of promoting germination to provide power, is bonded on seed coat after capable of being impregnated after rubber powder mixes, by purple
Outer light irradiation can promote the nutriment of neutron absorption soak, and the irradiation of ultraviolet light can make the activity for exciting embryo,
The stratification factor improves plantation germination percentage, shortens germinating time, can quickly judge whether not germinate to fill the gaps with seedlings in time, sends out
It is now that circulate operation as 2000-2500lx 3S- stopping irradiation 10S more effectively promotes to plant by irradiating ultraviolet light intensity
Son germination；After planting, seed needs to adapt to ambient enviroment and is incuded with itself environment, using similar in temperature and seed temperature
Culture substrate carries out just cultivation, and seed is more adaptable, and keeps the environment temperature of culture substrate periphery and culture medium temperature poor
One margin, so that slowly adaptive temperature reduces so as to ensure that seed will not seed after the temperature of culture substrate reduces
It initially senses the big difference of environment, guarantees that Interior Seed will not generate hormone response and cause suspend mode self-protection phenomenon, so as to
Enough guarantee the germination percentage of seed, and to control and not water immediately after planting, the original temperature of seed is reduced, so that seed is opened
Begin the temperature for just possessing germination, in the later period since the embedding seed of culture substrate will be in the at a temperature of life of an opposite constant temperature
It is long；When the root long of seed goes out, support seed being broken through into soil and grows seedling, the bud ratio of seed is low, also in that own
Embryo is short of power, and therefore, our additional promoting root growth liquid of application at this moment, fleabane flower extracting solution is by fleabane flower by conventional alcohol
Extracting solution is obtained after mentioning, and spends element, the additional basic element of cell division, gibberellin, auxin, molasses and jasmonic collective effect containing small cup
Promote the growth of root, molasses provide power element and also there is certain viscosity raw material is uniformly mixed and closely tie in promoting root growth liquid
It closes, the germination percentage of seed can be greatly improved after application.
In addition the culture substrate of the application with meet PH needed for kwangsi mayten herb germination process, carbon-nitrogen ratio, phosphorus, potassium with
And the needs of middle microelement, technological means of the EM bacterium Jing Guo the application can promote EM bacterium can a large amount of fast enrichings and activity
It is stronger, it is more thorough so as to improve each fermenting raw materials procedure decomposition, release the organic substance for being easier to absorb, micro member
Element etc., pomegranate leaf powder, leaf of Moringa powder, pokeberry root powder collective effect effectively raise the effect of sterilization.
[specific embodiment]
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, below with reference to of the invention specific
Embodiment is described in detail.In the following description, numerous specific details are set forth in order to facilitate a full understanding of the present invention.But
It is that the invention can be embodied in many other ways as described herein, those skilled in the art can be without prejudice to originally
Similar improvement is done in the case where invention intension, therefore the present invention is not limited to the specific embodiments disclosed below.
Embodiment 1:
1, raw material preparation:
Culture substrate is prepared in accordance with the following methods:
(1) EM bacterium is activated: slice is added androgynous ponding and stirs into mud after taking banana to remove the peel；It is sliced after taking sweet potato to remove the peel
Androgynous ponding is added afterwards and stirs into mud；It is sliced, dries after banana is removed the peel, crush, while conical flask is added in dehydrated potato powder
Enter in conical flask from conical flask entrance shower water, banaina and water are 1:5 according to weight ratio, then carry out boiling water bath boiling 20
Point, then three layers of filtered through gauze take filtrate, are then centrifuged filtrate using centrifuge, and 3000rpm is centrifuged 20S, takes supernatant
Liquid then carries out the concentrate for being concentrated into 30 ° of mother-in-law's U.S. degree, and the ethanol solution that mass fraction is 75% is then poured into side bevelling and is stirred
It mixes, then generates a large amount of flocculent deposit, be then -2 DEG C in temperature and freeze 5 hours, then reducing pressure is 140 pas, temperature
It is 5 hours dry at 28 DEG C, banana polysaccharide can be obtained.Using dehydrated potato powder being added and water can be improved the efficiency of extraction simultaneously
And recovery rate, recovery rate improve 0.5%；By leaf of bamboo clean dry, crushing, then by bamboo leaf powder be added 5 times of volumes distilled water,
Enter in conical flask while conical flask is added in bamboo leaf powder from conical flask entrance spray distilled water water, and in the case where power is 250W microwave
Processing 3 minutes, then starts boiling 25 minutes, filters to take filtrate, 1.11 times of original volume are concentrated under reduced pressure into, then in temperature
It is freezed 3 hours for -2 DEG C, is then centrifuged for machine 3000rpm and is centrifuged 5 minutes, take supernatant, dehydrated alcohol is then added and carries out precipitating 2
It is secondary, precipitating is taken by ultrafiltration membrance filter, after precipitating vacuum freeze-drying, obtains Polysaccharides in Bamboo Leaves.Using simultaneously be added bamboo leaf powder and
Water can be improved the efficiency and recovery rate of extraction, and recovery rate improves 0.3%；Banana puree 5g, sweet potato mash 3g are mixed with EM bacterium and stirred
It mixes and uniformly obtains mixed soil, after placing 1 minute, take conical flask that suitable quantity of water is added to add peptone 3g, beef extract 5g heating water bath
Constant temperature is completely dissolved to being stirred continuously after 40 DEG C to peptone, beef extract, after it is naturally cool be cooled to 35 DEG C after be added banana polysaccharide 2g,
Polysaccharides in Bamboo Leaves 1.5g, sodium chloride 3.5g and water are settled to 500ml, and then 30 DEG C, in revolution be that the activation of 30rpm/min shaking flask is 8 small
When；
(2) sugarcane top is crushed and sodium carboxymethylcellulose mixing is added, accumulation processing 36 hours, sodium carboxymethylcellulose
Additional amount is the 5% of sugarcane top weight；
(3) by chicken manure using ultraviolet light sterilize, exposure intensity 2500lx, irradiation turn over after ten minutes a chicken manure after
Continuous irradiation 10 minutes, repeatedly 3 times；
(4) by red soil, coal ash, middle sand, mushroom slag, sawdust, treated sugarcane top, treated chicken manure, rice washing water, bamboo
Heap fermentation is built in mixing after silk, EM bacterium weigh according to parts by weight, subsequent to fermentation material progress stirring to continue heap fermentation after fermentation 20 days
Processing 5 days, and keep the fermentation material relative humidity for building heap 30%, premix culture substrate can be obtained；
(5) boiling 10 in high-temperature steam cooker will be added after picking pomegranate leaf in time to divide, then take out and ground while hot
It is slurried, ethanol solution is added and carries out water-bath refluxing extraction 30 minutes, takes filtrate, filter residue continues cycling through refluxing extraction 3 times, merges filter
Then liquid is spray-dried to obtain pomegranate leaf powder；The volume ratio for the pomegranate leaf that ethanol solution and grinding are slurried is 5:1；Ethyl alcohol
The mass concentration of solution is 75%；The temperature of water-bath is 40 DEG C；
(6) it is dried after cleaning leaf of Moringa, 10 meshes is crossed after grinding, obtain leaf of Moringa powder；
(7) its root is taken after picking Phytolacca acinosa, is sliced, is dried naturally, powder is then polished into, and pokeberry root can be obtained
Powder；
(8) culture medium is can be obtained into premix culture substrate and pomegranate leaf powder, leaf of Moringa powder, pokeberry root powder after mixing
Matter.
2. applying the above-mentioned raw material being prepared in following cultural methods:
A kind of cultural method keeping kwangsi mayten herb characteristic, the specific steps are as follows:
The first step, the preparation of culture substrate: culture substrate in parts by weight, is prepared: red soil by following raw material
Soil 10 parts, 5 parts of coal ash, 8 parts of middle sand, 10 parts of mushroom slag, 5 parts of sawdust, 5 parts of sugarcane top, 5 parts of chicken manure, 5 parts of rice washing water, bamboo silk 7 parts,
0.5 part of EM bacterium, 1.5 parts of pomegranate leaf powder, 2.5 parts of leaf of Moringa powder, 1.5 parts of pokeberry root powder；
Seed preparation: second step the full kwangsi mayten herb seed of grain is washed with clean water, soak is then immersed in
In 10 minutes, then pull out, placement dry at normal temperature, the use of power is then 250W, at the ultrasonic wave that frequency is 200 hertz
Reason 0.5 minute is then that 2000lx irradiates 12S, the mode of operation of irradiation using ultraviolet ray intensity are as follows: irradiation 3S- stops irradiation
Seed is then placed at 32 DEG C and keeps the temperature preservation by 10S；
The soak is mixed to get by following raw material: water 100ml, bananas juice 20ml, bean sprout juice 15ml, tomato juice
10ml, rubber powder 1.5g；
Sprouting processing: culture substrate after culture substrate is carried out high-temperature sterilization, is laid in seedling bed immediately by third step
On, the thickness of culture substrate tiling is 15 centimetres, is 10 centimetres according to line-spacing for culture substrate and starts, and capable depth is 3
Centimetre, the temperature at detection culture substrate center reaches 30 DEG C, immediately by seed sowing in the row opened up, and every seed interval
10 centimetres, then backfill culture substrate, at being 25 DEG C in temperature, intensity of illumination 2000lx, light application time be 12 hours daily,
After sowing 3 days, start watering to keep the humidity of culture substrate to be 30%, water starts to pour promoting root growth liquid instead to protect after sowing 10 days
Holding culture substrate humidity is 30%, the germination until seed breaks ground；
The promoting root growth liquid is mixed to get by following raw material: basic element of cell division 5ml, gibberellin 3ml, auxin 8ml, oil lamp
Spend extracting solution 10ml, jasmonic 2.5ml, molasses 3g/ml, water 50ml；4th step, nursery is cultivated out: seedling after germination
After long to 5 centimetres, starting to seedling liquid fertilizer sprayed, 10Kg/ mus, other field management are normally carried out, weeding, insect prevention, until
Nursery out.
Embodiment 2:
1, raw material preparation:
Culture substrate is prepared in accordance with the following methods:
(1) EM bacterium is activated: slice is added androgynous ponding and stirs into mud after taking banana to remove the peel；It is sliced after taking sweet potato to remove the peel
Androgynous ponding is added afterwards and stirs into mud；It is sliced, dries after banana is removed the peel, crush, while conical flask is added in dehydrated potato powder
Enter in conical flask from conical flask entrance shower water, banaina and water are 1:5 according to weight ratio, then carry out boiling water bath boiling 30
Point, then three layers of filtered through gauze take filtrate, are then centrifuged filtrate using centrifuge, and 3500rpm is centrifuged 30S, takes supernatant
Liquid then carries out the concentrate for being concentrated into 30 ° of mother-in-law's U.S. degree, and the ethanol solution that mass fraction is 75% is then poured into side bevelling and is stirred
It mixes, then generates a large amount of flocculent deposit, be then -3 DEG C in temperature and freeze 8 hours, then reducing pressure is 155 pas, temperature
It is 6 hours dry at 28 DEG C, banana polysaccharide can be obtained.Using dehydrated potato powder being added and water can be improved the efficiency of extraction simultaneously
And recovery rate, recovery rate improve 0.8%；By leaf of bamboo clean dry, crushing, then by bamboo leaf powder be added 5 times of volumes distilled water,
Enter in conical flask while conical flask is added in bamboo leaf powder from conical flask entrance spray distilled water water, and in the case where power is 250W microwave
Processing 5 minutes, then starts boiling 35 minutes, filters to take filtrate, 1.11 times of original volume are concentrated under reduced pressure into, then in temperature
It is freezed 4 hours for -3 DEG C, is then centrifuged for machine 3500rpm and is centrifuged 8 minutes, take supernatant, dehydrated alcohol is then added and carries out precipitating 3
It is secondary, precipitating is taken by ultrafiltration membrance filter, after precipitating vacuum freeze-drying, obtains Polysaccharides in Bamboo Leaves.Using simultaneously be added bamboo leaf powder and
Water can be improved the efficiency and recovery rate of extraction, and recovery rate improves 0.6%；Banana puree 5g, sweet potato mash 3g are mixed with EM bacterium and stirred
It mixes and uniformly obtains mixed soil, after placing 1 minute, take conical flask that suitable quantity of water is added to add peptone 3g, beef extract 5g heating water bath
Constant temperature is completely dissolved to being stirred continuously after 40 DEG C to peptone, beef extract, after it is naturally cool be cooled to 35 DEG C after be added banana polysaccharide 2g,
Polysaccharides in Bamboo Leaves 1.5g, sodium chloride 3.5g and water are settled to 500ml, and then 32 DEG C, in revolution be that the activation of 40rpm/min shaking flask is 10 small
When；
(2) sugarcane top is crushed and sodium carboxymethylcellulose mixing is added, accumulation processing 58 hours, sodium carboxymethylcellulose
Additional amount is the 8% of sugarcane top weight；
(3) by chicken manure using ultraviolet light sterilize, exposure intensity 3000lx, irradiation turn over after ten minutes a chicken manure after
Continuous irradiation 10 minutes, repeatedly 5 times；
(4) by red soil, coal ash, middle sand, mushroom slag, sawdust, treated sugarcane top, treated chicken manure, rice washing water, bamboo
Heap fermentation is built in mixing after silk, EM bacterium weigh according to parts by weight, subsequent to fermentation material progress stirring to continue heap fermentation after fermentation 30 days
Processing 20 days, and keep the fermentation material relative humidity for building heap 40%, premix culture substrate can be obtained；
(5) boiling 20 in high-temperature steam cooker will be added after picking pomegranate leaf in time to divide, then take out and ground while hot
It is slurried, ethanol solution is added and carries out water-bath refluxing extraction 50 minutes, takes filtrate, filter residue continues cycling through refluxing extraction 5 times, merges filter
Then liquid is spray-dried to obtain pomegranate leaf powder；The volume ratio for the pomegranate leaf that ethanol solution and grinding are slurried is 8:1；Ethyl alcohol
The mass concentration of solution is 75%；The temperature of water-bath is 40 DEG C；
(6) it is dried after cleaning leaf of Moringa, 10 meshes is crossed after grinding, obtain leaf of Moringa powder；
(7) its root is taken after picking Phytolacca acinosa, is sliced, is dried naturally, powder is then polished into, and pokeberry root can be obtained
Powder；
(8) culture medium is can be obtained into premix culture substrate and pomegranate leaf powder, leaf of Moringa powder, pokeberry root powder after mixing
Matter.
2. applying the above-mentioned raw material being prepared in following cultural methods:
A kind of cultural method keeping kwangsi mayten herb characteristic, the specific steps are as follows:
The first step, the preparation of culture substrate: culture substrate in parts by weight, is prepared: red soil by following raw material
15 parts of soil, 10 parts of coal ash, 12 parts of middle sand, 15 parts of mushroom slag, 8 parts of sawdust, 10 parts of sugarcane top, 8 parts of chicken manure, 8 parts of rice washing water, bamboo silk 10
Part, 1.5 parts of EM bacterium, 2.5 parts of pomegranate leaf powder, 3.5 parts of leaf of Moringa powder, 3.5 parts of pokeberry root powder；
Seed preparation: second step the full kwangsi mayten herb seed of grain is washed with clean water, soak is then immersed in
In 15 minutes, then pull out, placement dry at normal temperature, the use of power is then 300W, at the ultrasonic wave that frequency is 300 hertz
Reason 1.5 minutes is then that 2500lx irradiates 15S, the mode of operation of irradiation using ultraviolet ray intensity are as follows: irradiation 3S- stops irradiation
Seed is then placed at 32 DEG C and keeps the temperature preservation by 10S；
The soak is mixed to get by following raw material: water 100ml, bananas juice 25ml, bean sprout juice 20ml, tomato juice
20ml, rubber powder 3.5g；
Sprouting processing: culture substrate after culture substrate is carried out high-temperature sterilization, is laid in seedling bed immediately by third step
On, the thickness of culture substrate tiling is 15 centimetres, is 10 centimetres according to line-spacing for culture substrate and starts, and capable depth is 5
Centimetre, the temperature at detection culture substrate center reaches 30 DEG C, immediately by seed sowing in the row opened up, and every seed interval
15 centimetres, then backfill culture substrate, at being 25 DEG C in temperature, intensity of illumination 2500lx, light application time be 12 hours daily,
After sowing 3 days, start watering to keep the humidity of culture substrate to be 40%, water starts to pour promoting root growth liquid instead to protect after sowing 12 days
Holding culture substrate humidity is 40%, the germination until seed breaks ground；
The promoting root growth liquid is mixed to get by following raw material: basic element of cell division 8ml, gibberellin 5ml, auxin 13ml, lamp
Small cup spends extracting solution 15ml, jasmonic 5.5ml, molasses 5g/ml, water 70ml；4th step, nursery is cultivated out: young after germination
After long to 10 centimetres of seedling, start to seedling liquid fertilizer sprayed, 20Kg/ mus, other field management are normally carried out, weeding, insect prevention, directly
To nursery out.
Embodiment 3:
1, raw material preparation:
Culture substrate is prepared in accordance with the following methods:
(1) EM bacterium is activated: slice is added androgynous ponding and stirs into mud after taking banana to remove the peel；It is sliced after taking sweet potato to remove the peel
Androgynous ponding is added afterwards and stirs into mud；It is sliced, dries after banana is removed the peel, crush, while conical flask is added in dehydrated potato powder
Enter in conical flask from conical flask entrance shower water, banaina and water are 1:5 according to weight ratio, then carry out boiling water bath boiling 24
Point, then three layers of filtered through gauze take filtrate, are then centrifuged filtrate using centrifuge, and 3450rpm is centrifuged 25S, takes supernatant
Liquid then carries out the concentrate for being concentrated into 30 ° of mother-in-law's U.S. degree, and the ethanol solution that mass fraction is 75% is then poured into side bevelling and is stirred
It mixes, then generates a large amount of flocculent deposit, be then -2 DEG C in temperature and freeze 6 hours, then reducing pressure is 150 pas, temperature
It is 5.3 hours dry at 28 DEG C, banana polysaccharide can be obtained.Using dehydrated potato powder being added and water can be improved the effect of extraction simultaneously
Rate and recovery rate, recovery rate improve 0.7%；By leaf of bamboo clean dry, crushing, then by the distillation of bamboo leaf powder 5 times of volumes of addition
Water enters in conical flask while conical flask is added in bamboo leaf powder from conical flask entrance spray distilled water water, and is that 250W is micro- in power
It is handled 4 minutes under wave, then starts boiling 30 minutes, filter to take filtrate, 1.11 times of original volume are concentrated under reduced pressure into, then in temperature
Degree freezes 3.5 hours for -3 DEG C, is then centrifuged for machine 3250rpm and is centrifuged 7 minutes, takes supernatant, and dehydrated alcohol is then added and carries out
Precipitating 3 times, takes precipitating by ultrafiltration membrance filter, after precipitating vacuum freeze-drying, obtains Polysaccharides in Bamboo Leaves.Using being added the leaf of bamboo simultaneously
Powder and water can be improved the efficiency and recovery rate of extraction, and recovery rate improves 0.55%；Banana puree 5g, sweet potato mash 3g and EM bacterium are mixed
Conjunction is uniformly mixing to obtain mixed soil, after placing 1 minute, takes conical flask that suitable quantity of water is added to add peptone 3g, beef extract 5g water-bath
Heated constant temperature is completely dissolved to being stirred continuously after 40 DEG C to peptone, beef extract, after it is naturally cool be cooled to 35 DEG C after that banana is added is more
Sugared 2g, Polysaccharides in Bamboo Leaves 1.5g, sodium chloride 3.5g and water are settled to 500ml, and then 31 DEG C, in revolution be that 35rpm/min shaking flask is living
Change 9 hours；
(2) sugarcane top is crushed and sodium carboxymethylcellulose mixing is added, accumulation processing 42 hours, sodium carboxymethylcellulose
Additional amount is the 6% of sugarcane top weight；
(3) by chicken manure using ultraviolet light sterilize, exposure intensity 2600lx, irradiation turn over after ten minutes a chicken manure after
Continuous irradiation 10 minutes, repeatedly 4 times；
(4) by red soil, coal ash, middle sand, mushroom slag, sawdust, treated sugarcane top, treated chicken manure, rice washing water, bamboo
Heap fermentation is built in mixing after silk, EM bacterium weigh according to parts by weight, subsequent to fermentation material progress stirring to continue heap fermentation after fermentation 26 days
Processing 12 days, and keep the fermentation material relative humidity for building heap 36%, premix culture substrate can be obtained；
(5) boiling 17 in high-temperature steam cooker will be added after picking pomegranate leaf in time to divide, then take out and ground while hot
It is slurried, ethanol solution is added and carries out water-bath refluxing extraction 39 minutes, takes filtrate, filter residue continues cycling through refluxing extraction 4 times, merges filter
Then liquid is spray-dried to obtain pomegranate leaf powder；The volume ratio for the pomegranate leaf that ethanol solution and grinding are slurried is 6:1；Ethyl alcohol
The mass concentration of solution is 75%；The temperature of water-bath is 40 DEG C；
(6) it is dried after cleaning leaf of Moringa, 10 meshes is crossed after grinding, obtain leaf of Moringa powder；
(7) its root is taken after picking Phytolacca acinosa, is sliced, is dried naturally, powder is then polished into, and pokeberry root can be obtained
Powder；
(8) culture medium is can be obtained into premix culture substrate and pomegranate leaf powder, leaf of Moringa powder, pokeberry root powder after mixing
Matter.
2. applying the above-mentioned raw material being prepared in following cultural methods:
A kind of cultural method keeping kwangsi mayten herb characteristic, the specific steps are as follows:
The first step, the preparation of culture substrate: culture substrate in parts by weight, is prepared: red soil by following raw material
Soil 13 parts, 7 parts of coal ash, 10 parts of middle sand, 14 parts of mushroom slag, 7 parts of sawdust, 6 parts of sugarcane top, 6 parts of chicken manure, 6 parts of rice washing water, bamboo silk 8 parts,
1 part of EM bacterium, 2 parts of pomegranate leaf powder, 3 parts of leaf of Moringa powder, 2 parts of pokeberry root powder；
Seed preparation: second step the full kwangsi mayten herb seed of grain is washed with clean water, soak is then immersed in
In 13 minutes, then pull out, placement dry at normal temperature, the use of power is then 280W, at the ultrasonic wave that frequency is 250 hertz
Reason 1 minute is then that 2700lx irradiates 14S, the mode of operation of irradiation using ultraviolet ray intensity are as follows: irradiation 3S- stops irradiation
Seed is then placed at 32 DEG C and keeps the temperature preservation by 10S；
The soak is mixed to get by following raw material: water 100ml, bananas juice 23ml, bean sprout juice 18ml, tomato juice
16ml, rubber powder 2.5g；
Sprouting processing: culture substrate after culture substrate is carried out high-temperature sterilization, is laid in seedling bed immediately by third step
On, the thickness of culture substrate tiling is 15 centimetres, is 10 centimetres according to line-spacing for culture substrate and starts, and capable depth is 4
Centimetre, the temperature at detection culture substrate center reaches 30 DEG C, immediately by seed sowing in the row opened up, and every seed interval
13 centimetres, then backfill culture substrate, at being 25 DEG C in temperature, intensity of illumination 2200lx, light application time be 12 hours daily,
After sowing 3 days, start watering to keep the humidity of culture substrate to be 37%, water starts to pour promoting root growth liquid instead to protect after sowing 11 days
Holding culture substrate humidity is 35%, the germination until seed breaks ground；
The promoting root growth liquid is mixed to get by following raw material: basic element of cell division 7ml, gibberellin 4ml, auxin 10ml, lamp
Small cup spends extracting solution 13ml, jasmonic 3.5ml, molasses 4g/ml, water 60ml；4th step, nursery is cultivated out: young after germination
After long to 7 centimetres of seedling, start to seedling liquid fertilizer sprayed, 14Kg/ mus, other field management are normally carried out, weeding, insect prevention, directly
To nursery out.
Test proves 1:
Comparative example 1:
Almost the same with the method for embodiment 3, difference is in the raw material of culture substrate without 2 parts of pomegranate leaf powder, Moringa
3 parts of leaf powder, 2 parts of pokeberry root powder.
Comparative example 2:
Almost the same with the method for embodiment 3, difference is in the raw material of culture substrate without 2 parts of pomegranate leaf powder, Phytolacca acinosa
2 parts of root powder.
Comparative example 3:
Almost the same with the method for embodiment 3, difference is in the raw material of culture substrate without 3 parts of stone leaf of Moringa powder, quotient
2 parts of land root powder.
The scheme of embodiment 1-3 and comparative example 1-3 is subjected to Experimental Comparison in certain precious farm of Baise moral and collects test
Data, are classified into 6 experimental plots, and each cell respectively sows 50 seeds in seedling bed at a seedling bed, and the sowing time is
On April in 2017 8.In the hair of on April 28th, 2017, on May 8th, 2017, on May 18th, 2017 to kwangsi mayten herb seed
Bud situation is recorded.As a result it see the table below:
Table 1
As seen from the above table, using technical solution pomegranate leaf powder, leaf of Moringa powder, the pokeberry root powder of the application, in contain ketone
Class, phenols, organic acid etc. have the function of antibacterial, and triple interaction can promote antibacterial prevention range and antibacterial width
Degree is capable of the continued growth of health after promoting seed to sprout.
Test proves 2:
Control group 1:
Almost the same with the method for embodiment 2, difference is that EM bacterium is activated: by banana puree 5g, sweet potato mash 3g and EM
Heating water bath constant temperature is to being stirred continuously to egg after 40 DEG C after bacterium adds suitable quantity of water to add peptone 3g, the mixing of beef extract 5g suitable quantity of water
White peptone, beef extract are completely dissolved, after it is naturally cool be cooled to 35 DEG C after be added banana polysaccharide 2g, Polysaccharides in Bamboo Leaves 1.5g, sodium chloride 3.5g
Be settled to 500ml with water, then 32 DEG C, revolution be 40rpm/min shaking flask activate 10 hours.
Control group 2:
Almost the same with the method for embodiment 2, difference is EM bacterium without activation processing.
Control group 3:
Almost the same with the method for embodiment 2, difference is culture substrate without 15 parts of red soil, 10 parts of coal ash, middle sand
12 parts, 15 parts of mushroom slag.
Control group 4:
Almost the same with the method for embodiment 2, difference is culture substrate without 8 parts of sawdust, 10 parts of sugarcane top, chicken manure 8
Part, 8 parts of rice washing water, 10 parts of bamboo silk.
Control group 5:
Almost the same with the method for embodiment 2, difference is that culture substrate is red soil+garden mould 1:1 ratio composition.
The culture medium that embodiment 1-3 and control group 1-5 are prepared carries out physicochemical property detection, and pH value uses pH acidity
The measurement of meter method, the content of organic matter are surveyed using the measurement of dichromic acid oxidation Outside Heating Method, total nitrogen content using Micro-kjoldahl method
Fixed, content of tatal phosphorus and full potassium content are measured using plasma emission spectrometry, quick-acting nitrogen contents are measured using alkaline hydrolysis-diffusion method,
Available phosphorus contents are using bisgallic acid extraction-plasma emission spectrometry measurement, quick-acting potassium content using ammonium acetate extraction-plasma hair
Penetrate spectrographic determination.
Table 2
| Full nitrogen g/kg | Full phosphorus g/kg | Full potassium g/kg | Available nitrogen mg/kg | |
| Embodiment 1 | 9.23 | 0.78 | 2.01 | 31.25 |
| Embodiment 2 | 10.02 | 0.85 | 2.12 | 32.25 |
| Embodiment 3 | 10.30 | 0.89 | 2.21 | 33.05 |
| Control group 1 | 9.09 | 0.68 | 1.74 | 25.68 |
| Control group 2 | 8.06 | 0.60 | 1.61 | 22.26 |
| Control group 3 | 7.98 | 0.61 | 1.76 | 26.21 |
| Control group 4 | 8.17 | 0.63 | 1.63 | 25.68 |
| Control group 5 | 5.36 | 0.54 | 1.33 | 19.36 |
| Rapid available phosphorus mg/kg | Available potassium mg/kg | PH value | Content of organic matter g/kg | |
| Embodiment 1 | 30.25 | 298.88 | 5.48 | 332.65 |
| Embodiment 2 | 31.26 | 301.25 | 5.62 | 348.64 |
| Embodiment 3 | 32.08 | 315.36 | 5.68 | 358.25 |
| Control group 1 | 27.65 | 265.48 | 5.20 | 302.52 |
| Control group 2 | 25.69 | 243.57 | 5.14 | 287.65 |
| Control group 3 | 26.95 | 255.36 | 5.03 | 289.69 |
| Control group 4 | 27.46 | 247.36 | 4.88 | 278.95 |
| Control group 5 | 22.36 | 215.26 | 4.69 | 269.25 |
Test proves 3:
By the seedling that the embodiment of the present application 1-3 is obtained and the kwangsi mayten herb that wild picking is arrived and after bud tissue cultures
Obtained kwangsi mayten herb is planted, picks the stem of kwangsi mayten herb respectively, maytenin is extracted using conventional extraction process, and
To 200 ± 10g of small white mouse, figure is close, the age in days after 1 tire of postpartum is identical, processing equally suffers from mammary cancer 1 00, is respectively divided into 5
Group, every group 20, using maytenin oral agents feed (embodiment 1-3, wild kwangsi mayten herb, tissue culture growth it is wide
The maytenin that western Caulis Mayteni extracts), 3 times a day, each 1g, 14 days as a treatment course check after treating a course for the treatment of
Breast cancer treatment effect；As a result it see the table below:
Table 3
As seen from the above table, contain maytenin amount using the kwangsi mayten herb that the application seeds cultivation comes out to be number two,
Therapeutic effect with it is wild close, than tissue cultures it is efficient improve it is very much.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitation of the scope of the invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection model of the invention
It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (5)
1. a kind of cultural method for keeping kwangsi mayten herb characteristic, which is characterized in that specific step is as follows:
The first step, the preparation of culture substrate: culture substrate in parts by weight, is prepared by following raw material: red soil 10-
15 parts, 5-10 parts of coal ash, sand 8-12 parts middle, 10-15 parts of mushroom slag, 5-8 parts of sawdust, 5-10 parts of sugarcane top, 5-8 parts of chicken manure, rice washing
5-8 parts of water, bamboo silk 7-10 parts, 0.5-1.5 parts of EM bacterium, 1.5-2.5 parts of pomegranate leaf powder, 2.5-3.5 parts of leaf of Moringa powder, pokeberry root powder
1.5-3.5 part；
Seed preparation: second step the full kwangsi mayten herb seed of grain is washed with clean water, is then immersed in soak
It 10-15 minutes, then pulls out, it is then 250-300W using power, frequency is 200-300 hertz that placement is dried at normal temperature
Ultrasonication 0.5-1.5 minutes, then using ultraviolet ray intensity be 2000-2500lx irradiate 12-15S, the operation of irradiation
Mode are as follows: irradiation 3S- stops irradiation 10S, and then seed is placed at 32 DEG C and keeps the temperature preservation；
The soak is mixed to get by following raw material: water 100ml, bananas juice 20-25ml, bean sprout juice 15-20ml, tomato juice
10-20ml, rubber powder 1.5-3.5g；
Third step, culture substrate: after culture substrate is carried out high-temperature sterilization, being laid on seedling bed by sprouting processing immediately, trains
The thickness for supporting matrix tiling is 15 centimetres, is 10 centimetres according to line-spacing for culture substrate and starts, and capable depth is 3-5 lis
The temperature of rice, detection culture substrate center reaches 30 DEG C, immediately by seed sowing in the row opened up, and every seed interval
10-15 centimetres, culture substrate is then backfilled, at being 25 DEG C in temperature, intensity of illumination 2000-2500lx, light application time 12
Hour daily, after sowing 3 days, starts watering and keeps the humidity of culture substrate for 30-40%, water starts after sowing 10-12 days
Pour promoting root growth liquid instead to keep culture substrate humidity for 30-40%, the germination until seed breaks ground；
The promoting root growth liquid is mixed to get by following raw material: basic element of cell division 5-8ml, gibberellin 3-5ml, auxin 8-13ml,
Fleabane flower extracting solution 10-15ml, jasmonic 2.5-5.5ml, molasses 3-5g/ml, water 50-70ml；
4th step, nursery is cultivated out: after germination after long to 5-10 centimetres of seedling, being started to seedling liquid fertilizer sprayed, 10-
20Kg/ mus, other field management are normally carried out, weeding, insect prevention, until nursery out.
2. a kind of cultural method for keeping kwangsi mayten herb characteristic according to claim 1, it is characterised in that: the culture
Matrix is prepared in accordance with the following methods:
(1) sugarcane top is crushed and sodium carboxymethylcellulose mixing is added, accumulation processing 36-58 hours, sodium carboxymethylcellulose
Additional amount is the 5-8% of sugarcane top weight；
(2) chicken manure is sterilized using ultraviolet light, a chicken manure is turned in exposure intensity 2500-3000lx, irradiation after ten minutes
Continue irradiation 10 minutes, repeatedly 3-5 times；
(3) by red soil, coal ash, middle sand, mushroom slag, sawdust, treated sugarcane top, treated chicken manure, rice washing water, bamboo silk,
Heap fermentation is built in mixing after EM bacterium weighs according to parts by weight, after fermentation 20-30 days, is carried out the subsequent heap of continuing of stirring to fermentation material and is fermented
Processing 5-20 days, and keep the fermentation material relative humidity for building heap in 30-40%, premix culture substrate can be obtained；
(4) boiling 10-20 points will be added in high-temperature steam cooker after picking pomegranate leaf in time, then takes out and is ground into while hot
Slurry is added ethanol solution and carries out water-bath refluxing extraction 30-50 minutes, takes filtrate, and filter residue continues cycling through refluxing extraction 3-5 times, closes
And filtrate, it is then spray-dried to obtain pomegranate leaf powder；
(5) it is dried after cleaning leaf of Moringa, 10 meshes is crossed after grinding, obtain leaf of Moringa powder；
(6) its root is taken after picking Phytolacca acinosa, is sliced, is dried naturally, powder is then polished into, and pokeberry root powder can be obtained；
(7) culture substrate is can be obtained into premix culture substrate and pomegranate leaf powder, leaf of Moringa powder, pokeberry root powder after mixing.
3. a kind of cultural method for keeping kwangsi mayten herb characteristic according to claim 2, it is characterised in that: in step
(4) in, ethanol solution and the volume ratio for grinding the pomegranate leaf being slurried are 5-8:1；The mass concentration of ethanol solution is 75%；Water-bath
Temperature be 40 DEG C.
4. a kind of cultural method for keeping kwangsi mayten herb characteristic according to claim 2, it is characterised in that: in step
(3) in, EM bacterium also first passes through activation processing.
5. a kind of cultural method for keeping kwangsi mayten herb characteristic according to claim 4, it is characterised in that: the activation
The temperature of processing is 30-32 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811161340.7A CN109302932A (en) | 2018-09-30 | 2018-09-30 | A kind of cultural method keeping kwangsi mayten herb characteristic |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811161340.7A CN109302932A (en) | 2018-09-30 | 2018-09-30 | A kind of cultural method keeping kwangsi mayten herb characteristic |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109302932A true CN109302932A (en) | 2019-02-05 |
Family
ID=65225309
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811161340.7A Pending CN109302932A (en) | 2018-09-30 | 2018-09-30 | A kind of cultural method keeping kwangsi mayten herb characteristic |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109302932A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283068A (en) * | 2011-07-04 | 2011-12-21 | 普洱市民族传统医药研究所 | Technology for artificially cultivating maytenus tree |
| CN103718694A (en) * | 2013-12-17 | 2014-04-16 | 广西壮族自治区中国科学院广西植物研究所 | Method for promoting seed germination of Kwangsi mayten herb |
| CN104255275A (en) * | 2014-10-15 | 2015-01-07 | 张家港贸安贸易有限公司 | Seed propagation and seedling technology of maytenus confertiflorus |
| CN105028942A (en) * | 2015-09-15 | 2015-11-11 | 管天球 | Ecological feed for rhizomys sinensis gray |
| CN107056350A (en) * | 2017-04-28 | 2017-08-18 | 德保县广鑫贸易有限公司 | A kind of high-yield planting method of selenium-rich soya bean |
| CN107197638A (en) * | 2017-06-22 | 2017-09-26 | 广西南宁荣威德新能源科技有限公司 | A kind of method for improving picria fel tarrae seed germination rate |
-
2018
- 2018-09-30 CN CN201811161340.7A patent/CN109302932A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283068A (en) * | 2011-07-04 | 2011-12-21 | 普洱市民族传统医药研究所 | Technology for artificially cultivating maytenus tree |
| CN103718694A (en) * | 2013-12-17 | 2014-04-16 | 广西壮族自治区中国科学院广西植物研究所 | Method for promoting seed germination of Kwangsi mayten herb |
| CN104255275A (en) * | 2014-10-15 | 2015-01-07 | 张家港贸安贸易有限公司 | Seed propagation and seedling technology of maytenus confertiflorus |
| CN105028942A (en) * | 2015-09-15 | 2015-11-11 | 管天球 | Ecological feed for rhizomys sinensis gray |
| CN107056350A (en) * | 2017-04-28 | 2017-08-18 | 德保县广鑫贸易有限公司 | A kind of high-yield planting method of selenium-rich soya bean |
| CN107197638A (en) * | 2017-06-22 | 2017-09-26 | 广西南宁荣威德新能源科技有限公司 | A kind of method for improving picria fel tarrae seed germination rate |
Non-Patent Citations (1)
| Title |
|---|
| 张钦德等: "《绿色道地药材规范化生产技新技术》", 31 May 2013, 山东人民出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103798024B (en) | A kind of implantation methods of celery | |
| CN105052479B (en) | A kind of beautiful millettia root seed seedling method on scale | |
| CN106718459A (en) | A kind of organic paddy rice implantation methods | |
| CN104255281A (en) | Sealwort cultivation method | |
| CN109220060A (en) | A kind of rhizoma polygonati seed seedling-raising method | |
| CN104350907A (en) | Technology for planting and disease control of fructus amomi | |
| CN103548441A (en) | Artificial seedling growing method of Gleditsia sinensis Lam seeds | |
| CN109247177A (en) | A kind of implantation methods improving kwangsi mayten herb emergence rate | |
| CN107371939A (en) | Planting method for improving yield of kiwi fruits | |
| CN106561428A (en) | Soilless culture method for pumpkins | |
| CN108812116A (en) | A kind of composite stereo ecology method for interplanting cultivation of capsicum, sponge gourd and water spinach | |
| CN106797783A (en) | A kind of cultural method of walnut forest interplanting radix bupleuri | |
| CN106665043A (en) | Tomato planting method | |
| CN106105992A (en) | A kind of method of Ramulus et folium taxi cuspidatae seminal propagation | |
| CN109496724B (en) | Efficient planting method for emilia sonchifolia | |
| CN107896890A (en) | The method for culturing seedlings and implantation methods of a kind of beautiful millettia root | |
| CN106912218A (en) | A kind of germination accelerating method for improving Hawthorn Seeds germination percentage | |
| CN106982722A (en) | The method for culturing seedlings of Momordica grosvenori | |
| CN107278571A (en) | Bletilla seedling field-transplanting method | |
| CN111011187A (en) | Method for rejuvenating and culturing rheum officinale seedlings | |
| CN106613833A (en) | Fiddlehead spore seedling culture method | |
| CN106069524A (en) | A kind of Tonnae Sinensis dwarfing planting method | |
| CN114731885B (en) | Seedling raising method for eucommia ulmoides | |
| CN105532219A (en) | Onion planting method capable of improving mouth-feel | |
| CN105493880A (en) | Green and high-yield tea-oil tree-tea tree-sweet potato-tarragon mixed planting method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190205 |
|
| WD01 | Invention patent application deemed withdrawn after publication |