CN109182166A - 一株具有缓解便秘功效的鼠李糖乳杆菌及其应用 - Google Patents
一株具有缓解便秘功效的鼠李糖乳杆菌及其应用 Download PDFInfo
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Abstract
一株具有缓解便秘功效的鼠李糖乳杆菌及其应用，菌株为鼠李糖乳杆菌Lactobacillus rhamnosus NCU2217，已于2018年4月9日保藏在中国微生物菌种保藏管理委员会普通微生物中心，地址：北京市朝阳区北辰西路1号院3号，中国科学院微生物研究所，其简称为CGMCC，保藏编号为CGMCC NO15573，本发明采用的鼠李糖乳杆菌CGMCC NO15573能够加快小肠蠕动并具有部分通便效果，便秘小鼠在摄入鼠李糖乳杆菌CGMCC NO15573后，乙酸、丙酸、正丁酸和总短链脂肪酸水平均有明显提高，短链脂肪酸总体含量增加能够降低肠道环境的pH值，结肠组织染色切片结果显示鼠李糖乳杆菌CGMCC NO15573具有修复结肠粘膜损伤的特性。
Description
技术领域
本发明属微生物领域，具体是涉及一株具有缓解便秘功效的鼠李糖乳杆菌 及其应用。
背景技术
鼠李糖乳杆菌是近年来国外研究较多的一种益生乳酸细菌，其广泛存在于 传统的发酵乳制品和人体胃肠道中。已有大量资料表明，鼠李糖乳杆菌在益生 菌领域有很广阔的开发及应用前景。目前研究结果已经证实鼠李糖乳杆菌具有 以下益生功能：(1)抗菌作用。报道称，鼠李糖乳杆菌对食物及肠道中的一些 致病菌具有抑制作用，其发酵所产生的抑菌物质对革兰氏阳性、阴性菌及多种 致病菌均有抑制作用；(2)抗氧化作用。研究表明，鼠李糖乳杆菌的菌悬液、 无细胞提取液及胞外多糖均具有较强的抗氧化活性；(3)免疫调节作用。鼠李 糖乳杆菌能够增强小鼠肠道及系统的免疫应答，并且对小鼠细胞的迟发型变态 反应、脾淋巴细胞增值能力以及NK细胞活性也具有显著效果；(4)治疗肠道 疾病。
发明内容
本发明的目的是提供一株具有缓解便秘功效的鼠李糖乳杆菌。
为实现以上目的，本发明提供了以下技术方案：
一株具有缓解便秘功效的鼠李糖乳杆菌及其应用，菌株为鼠李糖乳杆菌Lactobacillus rhamnosus NCU2217已于2018年4月9日保藏在中国微生物 菌种保藏管理委员会普通微生物中心，地址：北京市朝阳区北辰西路1号院3 号，中国科学院微生物研究所，其简称为CGMCC，保藏编号为CGMCC NO15573。 一株具有缓解便秘功效的鼠李糖乳杆菌及其应用：所述的鼠李糖乳杆菌 NCU2217为活菌或灭活菌或该菌株的衍生物。
一株具有缓解便秘功效的鼠李糖乳杆菌及其应用：所述应用为应用于食品领 域，所述的食品可以是发酵果蔬、发酵乳、乳酪、含乳饮料、乳粉或其他含有 该菌株或该菌株衍生物的食品中的任何一种。
一株具有缓解便秘功效的鼠李糖乳杆菌及其应用：本发明所提供的菌株及其组合物具有缓解便秘的功效，具体包括：对洛哌丁胺诱导的便秘小鼠的粪便指标 具有显著的缓解作用，可明显提高便秘小鼠的肠道蠕动水平，并促进结肠上皮 细胞与黏膜损伤的修复。
一株具有缓解便秘功效的鼠李糖乳杆菌及其应用：所述应用为：鼠李糖乳杆菌 以食品的形式组成组合物，发酵大宗果蔬益生菌发酵果蔬产品，包括：泡菜、 饮料、浆料，无需添加香精香料、色素、酸味剂、稳定剂、防腐剂食品添加剂， 所有风味由益生菌发酵果蔬原料产生。
本发明的有益效果：
鼠李糖乳杆菌CGMCC NO15573能够加快小肠蠕动并具有部分通便效果，便 秘小鼠在摄入鼠李糖乳杆菌CGMCC NO15573后，乙酸、丙酸、正丁酸和总短链 脂肪酸水平均有明显提高，短链脂肪酸总体含量增加能够降低肠道环境的pH 值，结肠组织染色切片结果显示鼠李糖乳杆菌CGMCC NO15573具有修复结肠粘 膜损伤的特性。
附图说明
图1鼠李糖乳杆菌CGMCC NO15573对便秘小鼠粪便短链脂肪酸和pH的影响。
图2结肠组织H&E染色病理学观察。
具体实施方式
实验例
下面结合附图和实施例、测试数据，对本发明进行具体描述：
选取造模成功的40只小鼠随机分成模型组(Constipation)、鼠李糖乳 杆菌CGMCCNO15573低剂量组(1.0×107CFU/mL)、鼠李糖乳杆菌CGMCC NO15573中剂量组(1.0×108CFU/mL)组和鼠李糖乳杆菌CGMCC NO15573高剂 量组(1.0×109CFU/mL)，按照10mL/kg的剂量给药，正常组(Normal)和 模型组灌胃同剂量生理盐水，连续灌胃15天，实验过程中，称量体重，并测 定采食量、饮水量，第15天给药后，6h内观察粪便性状，收集粪便，计数并 称重，收集的粪便置于真空冷冻干燥机内冻干，计算粪便含水量，(计算公式： 含水量(％)＝[粪便湿重(g)–粪便干重(g)]÷粪便湿重(g)×100) 最后一次给药24h后，灌胃墨汁，30min后脱锥处死，打开腹腔，分离小肠 肠管，将小肠在无张力情况下拉成直线进行测量肠管长度与墨汁推进长度。墨 汁推进率计算公式：墨汁推进率(％)＝墨汁推进长度(cm)÷小肠总长度(cm) ×100另外，取结肠组织并置于10％福尔马林固定液中固定，采用苏木素伊红(hematoxylin and eosin，H&E)染色进行病理学观察，实验结果见表1、2 和说明书附图1、2。
表1鼠李糖乳杆菌CGMCC NO15573干预对便秘小鼠饮食、饮水及粪便指标的影响
表2鼠李糖乳杆菌CGMCC NO15573对便秘小鼠肠道推进率的影响
结果显示，鼠李糖乳杆菌CGMCC NO15573各组小肠墨汁推进长度能够比便 秘模型组至少快47％，提示鼠李糖乳杆菌CGMCC NO15573能够加快小肠蠕动并 具有部分通便效果，此外，实验结果还显示便秘小鼠在摄入鼠李糖乳杆菌CGMCC NO15573后，乙酸、丙酸、正丁酸和总短链脂肪酸水平均有明显提高，短链脂 肪酸总体含量增加能够降低肠道环境的pH值。此外，结肠组织染色切片结果 还显示鼠李糖乳杆菌CGMCC NO15573具有修复结肠粘膜损伤的特性。
Claims (5)
1.一株具有缓解便秘功效的鼠李糖乳杆菌及其应用，菌株为鼠李糖乳杆菌Lactobacillus rhamnosus NCU2217，已于2018年4月9日保藏在中国微生物菌种保藏管理委员会普通微生物中心，地址：北京市朝阳区北辰西路1号院3号，中国科学院微生物研究所，其简称为CGMCC，保藏编号为CGMCC NO15573。
2.根据权利要求1所述的一株具有缓解便秘功效的鼠李糖乳杆菌及其应用，其特征在于：所述的鼠李糖乳杆菌NCU2217为活菌或灭活菌或该菌株的衍生物。
3.根据权利要求1所述的一株具有缓解便秘功效的鼠李糖乳杆菌及其应用，其特征在于：所述应用为应用于食品领域，所述的食品可以是发酵果蔬、发酵乳、乳酪、含乳饮料、乳粉或其他含有该菌株或该菌株衍生物的食品中的任何一种。
4.根据权利要求1所述的一株具有缓解便秘功效的鼠李糖乳杆菌及其应用，其特征在于：本发明所提供的菌株及其组合物具有缓解便秘的功效，具体包括：对洛哌丁胺诱导的便秘小鼠的粪便指标具有显著的缓解作用，可明显提高便秘小鼠的肠道蠕动水平，并促进结肠上皮细胞与黏膜损伤的修复。
5.根据权利要求1所述的一株具有缓解便秘功效的鼠李糖乳杆菌及其应用，其特征在于：所述应用为：鼠李糖乳杆菌以食品的形式组成组合物，发酵大宗果蔬益生菌发酵果蔬产品，包括：泡菜、饮料、浆料，无需添加香精香料、色素、酸味剂、稳定剂、防腐剂食品添加剂，所有风味由益生菌发酵果蔬原料产生。
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