CN109123287A - 一种菠萝灵芝菌丝球饮品及其制备方法 - Google Patents
一种菠萝灵芝菌丝球饮品及其制备方法 Download PDFInfo
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- CN109123287A CN109123287A CN201811010390.5A CN201811010390A CN109123287A CN 109123287 A CN109123287 A CN 109123287A CN 201811010390 A CN201811010390 A CN 201811010390A CN 109123287 A CN109123287 A CN 109123287A
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- pineapple
- ganoderma lucidum
- drink
- pompon
- lucidum pompon
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- A—HUMAN NECESSITIES
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- A01G18/40—Cultivation of spawn
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
本发明公开了一种菠萝灵芝菌丝球饮品及其制备方法,属于生物技术、饮料和食品技术领域。本发明的菠萝灵芝菌丝球饮品以工厂化深层发酵生产所获得的新鲜灵芝菌丝球为原料,以普通菠萝果汁饮料加工工艺为基础,将灵芝菌丝球与菠萝果汁饮料有机结合,所制得的产品不仅色、香、味俱佳,而且营养丰富,成本低廉,食用方便。该加工方法的特点是科学加入新鲜灵芝菌丝球,增加了饮料新品种,赋予了饮料新的保健功能,提升饮料品质,消费市场广阔。灵芝能够调理心脑血管、胃、肝、肾、肺、内分泌、气血不足、神经衰弱和亚健康,具有美容肌肤、延年益寿、增强免疫力、改善睡眠、舒筋活络、增强体质等功效。
Description
技术领域
本发明主要涉及生物技术、饮料和食品技术领域,具体涉及一种菠萝灵芝 菌丝球饮品及其制备方法。
背景技术
果汁是一类消费群体广、消费量大的休闲食品。但是,传统果汁以鲜果汁 或鲜果汁配以水和食糖等制成,功能性品种较少。因此,开发新的功能型果汁 产品,满足不同消费群体的需求十分必要。
菠萝营养丰富,含有果糖、葡萄糖、维生素B、维生C、磷、柠檬酸、脂肪、 纤维、尼克酸、钾、钠、锌、钙、磷、铁、胡萝卜素、和蛋白质等物质;菠萝 还含有一种叫菠萝阮酶物质,能分解蛋白质,帮助消化。菠萝性平,味甘、微 酸、微涩、性微寒,具有清暑解渴、消食止泻、补脾胃、固元气、益气血、消食、 祛湿、养颜瘦身等功效,为夏令医食兼优的时令佳果,不过一次也不宜吃太多。 目前对于菠萝主要是直接食用或者制作成菠萝罐头、菠萝汁饮料等,现有的工 艺相对较复杂,成本较高,且成分单一。
灵芝是药食两用名贵真菌,自古以来,一直被视为延年益寿的珍品,对于 慢性支气管炎、冠心病、肝炎、高血脂症、神经衰弱、肿瘤病的辅助治疗、白 细胞减少症等疾病有不同程度的疗效[曾祥丽等(2004)菌物研究,2(1):68~77], 其主要药效成分为灵芝多糖和三萜。灵芝多糖具有抗肿瘤、免疫调节、降血糖、 降血脂、抗氧化和抗衰老等作用[左军等(2015)中医药信息,32(5):122~123]; 灵芝三萜具有保肝、抗肿瘤、抗HIV~1及HIV~1蛋白酶活性、抑制组胺释放、 抑制血管紧张素转化酶、抑制胆固醇合成、镇痛、抑制血小板聚集、抗氧化等 作用[李国华等(2015)中草药,46(12):1858~1862]。然而,野生灵芝资源匮乏;种植灵芝周期长,占地广,且受季节影响。因此,原料成本高。此外,传 统灵芝以子实体或孢子入药,前者纤维化、木质化程度高,苦味浓;后者产量 低、细胞壁厚。因此,二者都不适宜于直接作为食品原料。
深层发酵生产灵芝菌丝球具有周期短、产率高、过程易控制、产品重量稳 定、易分离等特点,而且所生产灵芝菌丝球未木质化、苦味淡,营养价值及有效 成分不低于子实体甚至某些指标高于子实体。其中,菌丝球粗蛋白比子实体高 2.47倍,必需氨基酸占总氨基酸的比例二者相当,粗多糖及多糖含量分别为子 实体的2.26倍和3.5倍,有效成分易分离,易被人体吸收和利用,可直接用于 食品加工[张李阳等(1998)中国食用菌,17(1):15~16]。不同菌种菌丝球的三萜 含量在0.38%~1.34%之间[吴学谦等(2009)中国林副特产,(5):1~3],与子实 体的0.4%~1.19%[付立忠等(2009)中国食用菌,28(4):38~40]相差不大。因 此,菌丝球具有极高的开发利用价值。可见,深层发酵生产灵芝菌丝球为灵芝 原料大规模工厂化生产提供了可行途径。本发明利用果汁这一人们易于接受、 其本身又易于吸收的形式和优点,配以对人体具有营养和保健作用的灵芝菌丝 球,增加了产品中菌丝球蛋白、必需氨基酸及维生素的含量,弥补了果汁营养 缺乏的不足,既增加了果汁新品种,还赋予了果汁新的功能,消费市场广阔。
发明内容
本发明的目的在于克服上述现有技术的不足之处而提供一种含有灵芝菌丝 球的菠萝饮品及其制备方法。
为实现上述目的,本发明采用的技术方案为:一种菠萝灵芝菌丝球饮品, 所述菠萝灵芝菌丝球饮品包含以下重量百分比的成分:90~99.5%菠萝果汁和 0.5~10%灵芝菌丝球组合或90~99.5%菠萝果浆和0.5~10%灵芝菌丝球组合。
本发明的饮品以菠萝汁为主要原料,接入灵芝菌种后再通过深层发酵形成 另一个特色的结合体——菠萝灵芝液体菌种饮品,具有菠萝的营养成分,同时 也还有灵芝的营养成分。所以,形成的菠萝灵芝液体菌种饮品其营养及功效会 更加突出,此使普通的菠萝汁也由此增值百倍,本发明结合传统医学理论和现 代科学理论,将灵芝菌丝球和菠萝汁合理搭配,将灵芝通过深层发酵工艺获得 灵芝菌丝球,将原料中的物质成分彻底分解转化产生有益的营养成分;多种营 养物质融入产品中,营养平衡、系统补给,相互配合,针对性的调理、补充营 养,达到了最佳的食用效果;并且获得了独特的口感。本发明产品安全无毒,长期饮用具有改善睡眠、补充维生素、氨基酸、延缓机体衰老等保健功效。本 发明配方科学,技术先进,工艺简便,易于实现标准化、规范化、工厂化生产。 并通过发酵产生了卓越的香味和独特的口感,易于消费者接受。
作为本发明所述菠萝灵芝菌丝球饮品的优选实施方式,所述的菠萝果汁为 菠萝果原汁或调配菠萝果汁,所述菠萝果浆为菠萝果原浆或调配菠萝果浆。
作为本发明所述菠萝灵芝菌丝球饮品的优选实施方式,所述菠萝灵芝菌丝 球饮品包含以下重量百分比的成分:92~98%菠萝果汁和2~8%灵芝菌丝球组合 或92~98%菠萝果浆和2~8%灵芝菌丝球组合。
本申请发明人经过大量实验研究发现,所述的菠萝果原汁/原浆或调配菠萝 果汁/果浆与灵芝菌丝球在此重量范围内,其感官评价较佳。
作为本发明所述菠萝灵芝菌丝球饮品的优选实施方式,所述菠萝灵芝菌丝 球饮品包含以下重量百分比的成分:94~96%菠萝果汁和4~6%灵芝菌丝球组合 或92~98%菠萝果汁和2~8%灵芝菌丝球组合。
本申请发明人经过大量实验研究发现,所述的菠萝果原汁/原浆/或调配菠萝 果汁/果浆与灵芝菌丝球在此重量范围内,其感官评价更佳。
作为本发明所述菠萝灵芝菌丝球饮品的优选实施方式,所述菠萝灵芝菌丝 球饮品包含以下重量百分比的成分:95%菠萝果汁和5%灵芝菌丝球组合或95% 菠萝果桨和5%灵芝菌丝球组合。
本申请发明人经过大量实验研究发现,所述的菠萝果原汁/原浆/或调配菠萝 果汁/果浆与灵芝菌丝球在此重量范围内,其在口感、色泽、香气方面最佳。
本发明的另一目的还在于提供一种菠萝灵芝菌丝球饮品的制作方法,为实 现这一目的,本发明采取以下步骤:在菠萝果汁或菠萝果浆中加入灵芝菌丝球, 混合均匀,经脱气、灌装、杀菌、冷却、包装,得到所述菠萝灵芝菌丝球饮品。
作为本发明的所述菠萝灵芝菌丝球饮品的制作方法的优选实施方式,所述 灵芝菌丝球采用以下方法制成:将活化的灵芝菌种接种于液体发酵培养基中进 行发酵培养,分离获得灵芝菌丝球。
作为本发明的所述菠萝灵芝菌丝球饮品的制作方法的优选实施方式,所述 液体发酵培养包括以下浓度的组分:马铃薯200g/L,葡萄糖10g/L,酵母膏5g/L, KH2PO4 3g/L,MgSO4·7H2O 1.5g/L,维生素B1 2mg/L,70mL微量元素溶液, pH4.8。
作为本发明的所述灵菠萝芝菌丝球饮品的制作方法的优选实施方式,所述 微量元素溶液包括以下浓度的组分:MgSO4·7H2O 3g/L,MnSO4·H2O 0.5g/L, NaCl 1g/L,FeSO4·7H2O 0.1g/L,CoCl2 0.1g/L,ZnSO4·7H2O 0.1g/L,CuSO4·5H2O 0.1g/L,KA1(SO4)2·12H2O 0.01g/L,H3BO3 0.01g/L,Na2MoO4·2H2O 0.01g/L。
作为本发明的所述菠萝灵芝菌丝球饮品的制作方法的优选实施方式,所述 发酵培养是在发酵罐中进行的,所述发酵培养的条件为:所述液体发酵培养基 的体积为发酵罐容积的50~70%,发酵罐的罐压为0.3MPa,搅拌转速为100~200 r/min,通气量为1/5~3/5罐容积/min,自然pH,发酵培养的温度为28±2℃,所 述发酵培养的时间为5~10天。
与现有技术相比,本发明具有如下有益效果:
本发明产品在传统菠萝果汁生产的基础上,配以对人体具有营养和保健作 用的灵芝菌丝球加工而成,白色的菌丝球在饮料中均匀分散、晶莹闪亮,似小小 明珠,外观十分诱人;且风味独特、口感软弹、细腻;增加了产品中菌丝球蛋 白、必需氨基酸及维生素的含量,弥补了果汁营养因素缺乏的不足,既增加了 果汁新品种,还赋予了果汁新的功能。本发明产品符合现代人的对休闲食品的 需求,开辟了灵芝利用新方向。
具体实施方式
为了更加简洁明了的展示本发明的技术方案、目的和优点,下面结合具体 实施例对本发明做进一步的详细描述。
实施例1
(1)灵芝菌丝球的制备:以灵芝为出发菌株,将试管母种活化后接种至液 体发酵培养基,进行种子培养,然后转入发酵罐进行液体发酵,分离获得菌丝 球:
液体发酵培养基的配方:马铃薯200g/L,葡萄糖10g/L,酵母膏5g/L,KH2PO4 3g/L,MgSO4·7H2O 1.5g/L,维生素B1 2mg/L,70mL微量元素溶液(MgSO4·7H2O 3g/L,MnSO4·H2O0.5g/L,NaCl 1g/L,FeSO4·7H2O 0.1g/L,CoCl2 0.1g/L, ZnSO4·7H2O 0.1g/L,CuSO4·5H2O 0.1g/L,KA1(SO4)2·12H2O 0.01g/L,H3BO3 0.01g/L,Na2MoO4·2H2O 0.01g/L),pH4.8。
种子培养的条件:在摇床上进行振荡培养,培养室温度为20℃~30℃,培养 5天~10天;
发酵罐发酵的条件:以5%~15%的接种量将种子液转接入发酵罐,装液量 为罐容积的50%~70%,温度28℃±2℃,罐压0.3MPa,搅拌转速100r/min~200 r/min,通气量为1/5罐容积/min~3/5罐容积/min,自然pH。
(2)菠萝果汁的制备:
采用通用技术制备的各种菠萝果原汁(原浆)或调配菠萝果汁(浆)。
(3)菠萝灵芝菌丝球饮品的制备步骤如下:
A调配:菠萝灵芝菌丝球饮品的成分重量按100kg计,在95kg菠萝果汁/ 果浆,加入5kg灵芝菌丝球,搅拌均匀;
B装罐:将步骤A所得到的调配料液,根据需要装罐;
C脱气:将步骤B所得装罐料液用真空脱气机脱气10~30min,真空度为 0.05~1.00MP;
D杀菌:将步骤C所得料液封口进行巴氏杀菌,杀菌温度为80℃~85℃, 杀菌时间为8min~12min;
E冷却:将经骤D杀菌后的产品冷却至35℃以下;
F包装:根据需要,按照要求进行。本实施例的菠萝果汁/果浆可为调配菠 萝果汁/果浆或菠萝果原果汁/果浆。
实施例2
本实施例方法除菠萝果汁/果浆为90kg,灵芝菌丝球为10.0kg之外,其余步 骤与实施例1相同。本实施例的菠萝果汁/果浆可为调配菠萝果汁/果浆或菠萝果 原果汁/果浆。
实施例3
本实施例方法除菠萝果原汁/原浆为92kg,灵芝菌丝球为8.0kg之外,其余 步骤与实施例1相同。本实施例的菠萝果汁/果浆可为调配菠萝果汁/果浆或菠萝 果原果汁/果浆。
实施例4
本实施例方法除菠萝果原汁/果浆为94kg,灵芝菌丝球为6.0kg之外,其余 步骤与实施例1相同。本实施例的菠萝果汁/果浆可为调配菠萝果汁/果浆或菠萝 果原果汁/果浆。
实施例5
本实施例方法除菠萝果汁/果浆为95.5kg,灵芝菌丝球为4.5kg之外,其余 步骤与实施例1相同。本实施例的菠萝果汁/果浆可为调配菠萝果汁/果浆或菠萝 果原果汁/果浆。
实施例6
本实施例方法除菠萝果果汁/果浆为96kg,灵芝菌丝球为4.0kg之外,其余 步骤与实施例1相同。本实施例的菠萝果汁/果浆可为调配菠萝果汁/果浆或菠萝 果原果汁/果浆。
实施例7
本实施例方法除菠萝果汁/果浆为98kg,灵芝菌丝球为2.0kg之外,其余步 骤与实施例1相同。本实施例的菠萝果汁/果浆可为调配菠萝果汁/果浆或菠萝果 原果汁/果浆。
实施例8
本实施例方法除菠萝果汁/果浆为99.5kg,灵芝菌丝球为0.5kg之外,其余 步骤与实施例1相同。
对比例1
本对比例与实施例1不同之处仅在于,菠萝果汁/果浆重量百分比为88%, 灵芝菌丝球的重量百分比为12%。
对比例2
本对比例与实施例1不同之处仅在于,菠萝果汁/果浆重量百分比为100%, 灵芝菌丝球的重量百分比为0%。
实施例9菠萝灵芝菌丝球饮品的各项理化指标
取实施例1至8制备的菠萝灵芝菌丝球饮品按GB/47892~2010、GB 4789.3~2010、GB/T5009.11~1996、GB/T7500899112~1996、GB/T5009.13~1996 检测标准比较各项指标,结果见表1。
表1菠萝灵芝菌丝球饮品的各项理化指标
从表1中可看出,本发明的菠萝灵芝菌丝球饮品的各项理化指标均符合标 准。
实施例10菠萝灵芝菌丝球饮品的感官评价
对实施例1至8制备的菠萝灵芝菌丝球饮品以及对比例进行感官评价,经 选取有丰富饮料品尝经验的60人进行品尝比较实施例1至8和对比例1至2的 饮料,采用不记名打分制,分别从产品的色泽、口感、香气、喜好度进行评分, 每项满分为10分,色泽以白色的菌丝球在饮料中均匀分散、晶莹闪亮,似小小 明珠为10分;香气以独特的菠萝味和淡淡的灵芝味为10分;口感以酸甜爽口、 细腻顺滑为10分,分数越高说明效果越好,并对是否喜欢产品的程度进行总体 评价,结果如表2所示:
表2感官评定结果
从表2可看出,本发明所制备的菠萝灵芝菌丝球饮品,在口感、色泽和香 气均得到了认可,对比例1中饮品的成分的含量在本发明的范围之外,对比例2 的饮品不含有灵芝菌丝球成分,而从结果可看出,所制得的饮品与对比例相比, 在色泽、口感、香气方面认可度明显更高,可见,本发明在两种成分共同搭配, 以及在本发明的配比范围内,其效果最佳,且在番石榴果汁添加灵芝菌丝球, 在色泽、口感、香气方面均得到提升,而以实施例5的效果最佳,其显著高于 其他实施例,这是本申请发明人意外发现的。
实施例11对菠萝灵芝菌丝球饮品部分成分分析
样品:本实施例5制备的菠萝灵芝菌丝球饮品。
成分分析方法:总糖(多糖)、粗三萜、总酚、粗蛋白、粗脂肪、微量元素、 氨基酸
1)总糖(多糖)测定:采用苯酚硫酸法。
2)还原糖测定:采用DNS法(3,5~二硝基水杨酸比色法)。
3)粗脂肪测定:取50g菠萝灵芝菌丝球饮品原浆,冷冻干燥后研细,全部移 入滤纸筒内,并将滤纸筒放入脂肪抽提器的抽提桶中。加入乙醚,在60℃下回 流抽提8小时。抽提完全后,将脂肪接受瓶置于101℃~105℃烘箱中烘干至恒重, 并记录重量。
4)粗蛋白测定:取50g菠萝灵芝菌丝球饮品原浆,冷冻干燥后研细。采用 凯氏定氮法测样品中粗蛋白含量。
5)粗三萜含量的测定:①乙醇提取物的制备。取200g菠萝灵芝菌丝球饮品 原浆,冷冻干燥后研成细粉。干粉与乙醇以(0.1~1)g:20mL混合,搅拌2小 时,静置过夜。用抽滤和倒吸的方法吸出滤液(上层清液用倒吸法,下层用抽滤 法)。如此反复抽提3次,合并滤液后于40~45℃减压浓缩。将浓缩得到的粘稠 浸膏用真空干燥箱干燥,得到乙醇提取物膏状物。②标准曲线的制作。将齐墩 果酸(≥98%,中药固体制剂研究中心)用甲醇配成0.2mg/mL的标准溶液,精 密吸取该液0、0.2、0.3、0.4、0.5、0.6、0.7和0.8mL,分别置于具塞试管中, 挥去溶剂,准确加入5%香草醛的冰醋酸溶液0.4mL、高氯酸1.6mL,混匀,置 70℃恒温水浴中加热15分钟,冷却至室温,并转移至10mL容量瓶中加乙酸乙 酯稀释至刻度,摇匀,在560nm波长处测定光吸收值,以齐墩果酸重量为横坐 标(x),吸光值为纵坐标(y)做标准曲线,求出回归方程:y=8.047x+0.0248, r2=0.9991(n=6)。③粗三萜含量测定方法。取浓度为1mg/mL的乙醇提取物 0.5mL,按标准曲线的测定方法,测出其在560nm下的OD560值,根据标准曲 线计算乙醇提取物中粗三萜的重量,按下式求出各提取物粗三萜的含量:
乙醇提取物粗三萜含量(%)=m/c×v。其中:m为供试液中粗三萜总量(mg); c为供试液的浓度(1mg/mL);v为供试液体积(mL)。再计算出杏鲍菇发酵饮料原 浆粗三萜含量(%,mg/g)。
6)总酚含量的测定:①菠萝灵芝菌丝球饮品原浆提取物制备同上。②标准曲 线的制作。没食子酸(≥98%,中药固体制剂研究中心)为标准品,将没食子酸用 甲醇配成0.1mg/mL的标准溶液,精密吸取该液0、10、20、30、40、50、 60、70、80、90、100μg/mL,分别加入1mL Folin~酚试剂,室温放置3~4min, 再加入10%Na2CO3饱和溶液1mL,用H2O稀释至10mL,在暗处反应90min后, 在760nm处测定其吸收值。以没食子酸的重量为横坐标(x),吸光值为纵坐标(y) 做标准曲线,求出回归方程:y=0.0097x+0.114,r2=0.9956(n=6)。③总酚含量的测 定方法。按标准曲线的测定方法,取浓度为1mg/mL的乙醇提取物1mL,测其在760nm下的OD760值,根据标准曲线计算各提取物中总酚的百分含量。乙醇 提取物中总酚含量(%)=m/c×v,其中:m为供试液中总酚总量(mg);c为供试液 的浓度(1mg/mL);v为供试液体积(mL)。再计算菠萝灵芝菌丝球饮品原浆总酚含 量(%,mg/g)。
7)微量元素分析:铜、铁、锌采用火焰原子吸收光谱法。含量低的砷、硒、 铅用电感耦合等离子体质谱仪进行检测。
8)氨基酸含量测定。取100g菠萝灵芝菌丝球饮品原浆,冷冻干燥后研细, 送国家饲料重量监督检验中心检测。
粗纤维测定采用称重法,即饮料原浆过滤后的干物质重量。
维生素C、维生素B1、维生素B2的测定方法参照食品安全国家标准进行。GB5413.18~2010(维生素C);GB 5413.11~2010(维生素B1);GB5413.12~2010(维 生素B2)。
结果菠萝灵芝菌丝球饮品原浆主要营养成分见表3。
表3灵芝发酵饮料原浆主要营养成分(%)
从表3可知,菠萝灵芝菌丝球饮品主要营养成分非常丰富,含对人体有营 养价值的糖类、粗蛋白、粗脂肪和维生素类。此外,还含具有抗氧化、抗肿瘤 功效的粗三萜和酚类物质。
菠萝灵芝菌丝球饮品微量元素检测结果:灵芝发酵饮料原浆微量元素检测 结果表明,饮料原浆含有对人体有益的元素硒35μg/mL、锌100μg/mL、铁 210μg/mL;未检测到有害重金属离子。
菠萝灵芝菌丝球饮品氨基酸种类及含量分析,菠萝灵芝菌丝球饮品氨基酸 种类及含量分析分析见表4。
表4菠萝灵芝菌丝球饮品氨基酸种类及含量分析(%,mg/g)
氨基酸 | 含量 | 氨基酸 | 含量 | 氨基酸 | 含量 |
天冬氨酸 | 2.51 | 酪氨酸 | 0.63 | 组氨酸 | 0.21 |
谷氨酸 | 6.43 | 脯氨酸 | 0.81 | 甘氨酸 | 1.32 |
丙氨酸 | 2.03 | 胱氨酸 | 0.83 | 亮氨酸 | 0.68 |
精氨酸 | 3.47 | 苏氨酸 | 1.37 | 色氨酸 | 1.54 |
蛋氨酸 | 0.23 | 异亮氨酸 | 0.67 | 缬氨酸 | 1.04 |
苯丙氨酸 | 1.57 | 赖氨酸 | 0.63 | ||
必须氨基酸 | 7.73 | 氨基酸总量 | 23.97 |
从表4可看出,本实施例5的饮品中不仅氨基酸种类齐全,而且含人体必 需的8种氨基酸。
应用例1
本发明的饮品不仅具有良好的口感、香气,其对人体具有营养和保健作用;
选择患有失眠、慢性胃炎、易感冒21人,连续服用实施例5的饮料30天, 每天服用500ml,期间不改变原来的饮食习惯,正常饮食。发现饮用后烦躁、精 神不集中、心慌等症状均有改善;饮用后较饮用前睡眠明显改善,精神气足, 胃酸明显减轻;饮用前后心率无显著改变。
以上所述仅为本发明的较佳实施例而已,并不用以限制本发明,凡在本发 明的精神和原则之内,所做的任何修改、等同替换、改进等,均应包含在本发 明保护的范围之内。
Claims (10)
1.一种菠萝灵芝菌丝球饮品,其特征在于,所述菠萝灵芝菌丝球饮品包含以下重量百分比的成分:90~99.5%菠萝果汁和0.5~10%灵芝菌丝球组合或90~99.5%菠萝果浆和0.5~10%灵芝菌丝球组合。
2.根据权利要求1所述的菠萝灵芝菌丝球饮品,其特征在于,所述的菠萝果汁为菠萝果原汁或调配菠萝果汁,所述菠萝果浆为菠萝果原浆或调配菠萝果浆。
3.根据权利要求2所述的菠萝灵芝菌丝球饮品,其特征在于,所述菠萝灵芝菌丝球饮品包含以下重量百分比的成分:92~98%菠萝果汁和2~8%灵芝菌丝球组合或92~98%菠萝果浆和2~8%灵芝菌丝球组合。
4.根据权利要求2所述的菠萝灵芝菌丝球饮品,其特征在于,所述菠萝灵芝菌丝球饮品包含以下重量百分比的成分:94~96%菠萝果汁和4~6%灵芝菌丝球组合或92~98%菠萝果汁和2~8%灵芝菌丝球组合。
5.根据权利要求2所述的菠萝灵芝菌丝球饮品,其特征在于,所述菠萝灵芝菌丝球饮品包含以下重量百分比的成分:95%菠萝果汁和5%灵芝菌丝球组合或95%菠萝果桨和5%灵芝菌丝球组合。
6.一种根据权利要求1~5任一所述的菠萝灵芝菌丝球饮品的制备方法,其特征在于:在菠萝果汁或菠萝果浆中加入灵芝菌丝球,混合均匀,经脱气、灌装、杀菌、冷却、包装,得到所述菠萝灵芝菌丝球饮品。
7.根据权利要求6所述的菠萝灵芝菌丝球饮品的制备方法,其特征在于,所述菠萝灵芝菌丝球采用以下方法制备得到:将活化的灵芝菌种接种于液体发酵培养基中进行发酵培养,分离获得灵芝菌丝球。
8.根据权利要求7所述菠萝灵芝菌丝球饮品的制备方法,其特征在于,所述液体发酵培养包括以下浓度的组分:马铃薯200g/L,葡萄糖10g/L,酵母膏5g/L,KH2PO4 3g/L,MgSO4·7H2O 1.5g/L,维生素B1 2mg/L,70mL微量元素溶液,pH4.8。
9.根据权利要求8所述菠萝灵芝菌丝球饮品的制备方法,其特征在于,所述微量元素溶液包括以下浓度的组分:MgSO4·7H2O 3g/L,MnSO4·H2O 0.5g/L,NaCl 1g/L,FeSO4·7H2O0.1g/L,CoCl2 0.1g/L,ZnSO4·7H2O 0.1g/L,CuSO4·5H2O 0.1g/L,KA1(SO4)2·12H2O0.01g/L,H3BO3 0.01g/L,Na2MoO4·2H2O 0.01g/L。
10.根据权利要求7所述菠萝灵芝菌丝球饮品的制备方法,其特征在于,所述发酵培养是在发酵罐中进行的,所述发酵培养的条件为:所述液体发酵培养基的体积为发酵罐容积的50~70%,发酵罐的罐压为0.3MPa,搅拌转速为100~200r/min,通气量为1/5~3/5罐容积/min,自然pH,发酵培养的温度为28±2℃,所述发酵培养的时间为5~10天。
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