CN109005966A - A kind of cuttage breeding method of Gesneriaceae - Google Patents
A kind of cuttage breeding method of Gesneriaceae Download PDFInfo
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- CN109005966A CN109005966A CN201810752459.5A CN201810752459A CN109005966A CN 109005966 A CN109005966 A CN 109005966A CN 201810752459 A CN201810752459 A CN 201810752459A CN 109005966 A CN109005966 A CN 109005966A
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- Prior art keywords
- cuttage
- gesneriaceae
- breeding method
- aqueous solution
- cuttage breeding
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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Abstract
The present invention provides a kind of cuttage breeding methods of Gesneriaceae, belong to field of plant growing technology.The method of the invention is the following steps are included: enter matrix, 15~30d of culture for the Gesneriaceae mature tissue cuttage for dipping water of taking root;After the cuttage, into the matrix, every 5~9d pours a growth regulator aqueous solution;The effective component of the growth regulator aqueous solution includes blue powder disinfectant and root-inducing powder.Utilize method of the invention, after 25~30d of cuttage culture, rooting rate is 90%~100%, and adventitious bud germination percentage is 100%, the rooting rate and adventitious bud germination percentage of Gesneriaceae explant can be significantly improved, the cost of Gesneriaceae large-scale production is reduced.
Description
Technical field
The invention belongs to field of plant growing technology, and in particular to a kind of cuttage breeding method of Gesneriaceae.
Background technique
Gesneriaceae (Gesneriaceae) plant about 150 belongs to 3700 kinds, is distributed widely in tropical and subtropical region, I
State about 59 belongs to 493 kinds, is distributed mainly on the Changjiang river areas to the south.Section's plant flower type variation multiplicity, ornamental value height, leaf color spot
It refutes, plant type rounding or shape of crawling, and shade tolerance with higher.Primulina tabacum category is excellent ornamental flower resource, most of
Primulina tabacum platymiscium is alternatively arranged as medicinal;Hemiboea, Lysionotus are precious resources of medicinal plant, and lipstick flower belongs to
It is emerging ornamental flower on the market, there is great value of exploiting and utilizing, Petrocosmea plant type is graceful, exquisite unique, has huge
Big market developing and utilizingpotentiality.Other ornamental class Gesneriaceae such as African violet categories, gorgeous spot lettuce tongue fur category, gloxinia category
Deng prevailing already on flowers market, there is certain application value in landscape application.Wherein African violet
(Saintpaulia), gloxinia (Sinningia speciosa), lipstick flower (Aeschynanthuspulche) etc. are flowers
Commodity potted flower extremely popular in market, and perfect merchandized handling system is had been set up, but the ornamental plant excellent to other
Object germ plasm resource does not carry out commercialized development.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of Conandron ramondioides sieb. Et zuce can plant cuttage breeding method, it is easy, fast
It is fast, efficient, the rooting rate and adventitious bud germination percentage of Gesneriaceae explant are significantly improved, to improve cutting propagation
Success rate, improve input-output ratio.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of cuttage breeding methods of Gesneriaceae, comprising the following steps: will dip water of taking root
The cuttage of Gesneriaceae mature tissue enter matrix, cultivate 15~30d;
After the cuttage, into the matrix, every 5~9d pours a growth regulator aqueous solution;The growth regulating
The effective component of agent aqueous solution includes blue powder disinfectant and root-inducing powder.
Preferably, the environment of the culture is the greenhouse of sunshade, ventilation.
Preferably, the Gesneriaceae mature tissue includes mature leaf, blade-carrying petiole or stem section.
Preferably, the water of taking root is the aqueous solution for including heteroauxin and/or Nafusaku.
Preferably, the concentration of heteroauxin and/or Nafusaku is 0.2~1% in the water of taking root.
Preferably, the mode of the cuttage includes oblique cutting.
Preferably, the temperature of the culture is 18~32 DEG C.
Preferably, the air humidity of the culture is 50~95%.
Preferably, the effective component in the growth regulator aqueous solution is diluted with water 1000 times.
Preferably, the concentration of blue powder disinfectant and root-inducing powder ratio is 1 in the effective component of the growth regulator aqueous solution:
1。
The present invention provides a kind of cuttage breeding methods of Gesneriaceae, are outer with Gesneriaceae mature tissue
Implant carries out cuttage, and the rooting rate and adventitious bud germination percentage of Gesneriaceae explant can be improved, meanwhile, the Conandron ramondioides sieb. Et zuce of preparation
Section's plant seedlings high survival rate, it is easy to operate, production cost is reduced, facilitates Gesneriaceae and large-scale develops and utilizes.This
In inventive embodiments, after cuttage culture 25~30 days, rooting rate is 90~100%, adventitious bud germination percentage 100%.
Specific embodiment
The present invention provides a kind of cuttage breeding methods of Gesneriaceae, comprising the following steps: will dip water of taking root
The cuttage of Gesneriaceae mature tissue enter matrix, cultivate 15~30d;
After the cuttage, into the matrix, every 5~9d pours a growth regulator aqueous solution;The growth regulating
The effective component of agent aqueous solution includes blue powder disinfectant and root-inducing powder.
In the cuttage breeding method of Gesneriaceae of the present invention, the Gesneriaceae for dipping water of taking root is planted first
The cuttage of object mature tissue enters matrix.Gesneriaceae mature tissue of the present invention preferably includes mature leaf, with blade
Petiole or stem section.There is no particular determinations in source of the present invention to the Gesneriaceae mature tissue, are preferably derived from
Robust growth, disease-free maternal plant.When using blade-carrying petiole as explant, the present invention is preferably directly cut on maternal plant
Petiole base is taken, it is described to cut off part leaf margin, to reduce transpiration;When the present invention is to using mature leaf or stem section as explant
There is no particular determinations for acquisition modes.
The explant is dipped water of taking root by the present invention, and the effective component of the water of taking root preferably comprises heteroauxin
And/or Nafusaku, more preferably heteroauxin or Nafusaku, most preferably heteroauxin.In water of the present invention of taking root
The concentration of effective component is preferably 0.2%~1%, and more preferably 0.3%~0.8%, most preferably 0.5%.The present invention is to institute
Stating the mode that dips of water of taking root, there is no particular determinations, and the explant is preferably dipped in 3~10s in the water of taking root.
The present invention enters the explant cuttage for dipping water of taking root in matrix, and the mode of the cuttage preferably includes oblique cutting.This
Invent the mixture that the matrix preferably comprises perlite and vermiculite, the volume ratio of the perlite and vermiculite is preferably 1:
(0.6~2), more preferably 1:(0.8~1.5), most preferably 1:1.The matrix is preferably filled in hole tray by the present invention, institute
Stating hole tray is preferably 36 hole hole trays.When the present invention carries out cuttage as explant using mature leaf, when leaf area is larger, such as tobacco leaf report
Spring lettuce tongue fur, Guilin primulina tabacum etc. form wound preferably at vein, and blade are tiled in matrix, cover institute in wound
State matrix.The quantity of wound described in every leaf of the present invention is preferably 1~2.The present invention to the forming method of the wound not
There is particular determination, preferably breaks and disconnected form wound.
The present invention cultivates 15~30d, the environment of the culture is preferably the greenhouse of sunshade, ventilation after cuttage.?
In the present invention, the temperature of the culture is preferably 18~32 DEG C, more preferably 24~29 DEG C, most preferably 25~28 DEG C;It is described
The relative humidity of culture is preferably 50~95%, and more preferably 60~85%, most preferably 65~80%.
The present invention is after the cuttage, and into the matrix, every 5~9d pours a growth regulator aqueous solution;The life
The effective component of long regulator aqueous solution includes blue powder disinfectant and root-inducing powder.It is blue in growth regulator aqueous solution of the present invention
The concentration of powder disinfectant and root-inducing powder is than being preferably 1:(0.6~1.5), more preferably 1:(0.8~1.2), most preferably 1:1,
When in use, the concentration of blue powder disinfectant and root-inducing powder is 1000 times of aqueous solutions in the growth regulator aqueous solution.This hair
There is no particular determinations in bright source and type to blue powder disinfectant and root-inducing powder in the growth regulator aqueous solution.The present invention
The amount of spraying of the growth regulator aqueous solution preferably irrigates matrix.Growth regulator aqueous solution of the present invention sprays frequency
Rate is preferably 6~8d primary, and more preferably 7d is primary.
The present invention preferably further includes the process of anti-moisture evaporation after spraying the growth regulator aqueous solution, described
Preferably by plastic covering lid, the plastic lid is preferably the pallet cover with vent valve for anti-moisture evaporation, and it is big that stomata is adjusted
It is small, prevent moisture from evaporating.
Present invention 15d or so after the cuttage, that is, can produce callus, and differentiate adventitious root and bud, formed
Whole plant.
It is described in detail below with reference to cuttage breeding method of the embodiment to Gesneriaceae provided by the invention,
But they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
Take adult petiole base of the primulina tabacum category with blade as explant, explant is dipped in water of taking root, and (concentration is
0.5% heteroauxin solution), the oblique cuttage of the petiole is entered into the matrix that perlite and vermiculite (1: 1, v/v) mix and (is loaded on 36 holes
In hole tray) in, blade is stood on matrix face, and explant cuttage is after matrix, by blue powder disinfectant and root-inducing powder mixed preparing
1000 times of aqueous solutions are sprayed in 36 hole plug medias, matrix to be irrigated, and cover adjustable pore opening has airing function
Pallet cover, cuttage growing environment is sunshade, in ventilative, ventilation greenhouse, and control environment temperature is 26~28 DEG C, relatively
Humidity is 65~80%, pours an aqueous solution week, matrix is irrigated.After culture 30 days, rooting rate 100% is indefinite
Bud germination percentage 100%.
Comparison: in addition to spraying for tap water, remaining operation is same as Example 1, and after cultivating same time, rooting rate is
30%, adventitious bud germination percentage 50%.
Embodiment 2
It takes the blade of adult gloxinia as explant, explant is dipped in into water of the taking root (heteroauxin that concentration is 0.5%
Solution), the oblique cuttage of the blade is entered in the matrix that perlite and vermiculite (1: 1, v/v) mix, by blue powder disinfectant and root-inducing powder
1000 times of aqueous solutions of mixed preparing, are sprayed in matrix, matrix to be irrigated, and cover adjustable pore opening has airing function
Pallet cover, cuttage growing environment is sunshade, in ventilative, ventilation greenhouse, and control environment temperature is 26~28 DEG C, relatively
Humidity is 65~80%, pours an aqueous solution week, matrix is irrigated.After culture 25 days, rooting rate 100% is indefinite
Bud germination percentage 100%.
Comparison: in addition to spraying for tap water, remaining operation is same as Example 2, and after cultivating same time, rooting rate is
20%, adventitious bud germination percentage 30%.
Embodiment 3
It takes the stem section of adult Hemiboea as explant, explant is dipped in into water of the taking root (indoles that concentration is 0.5%
Acetic acid solution), the oblique cuttage of the stem section is entered in the matrix that perlite and vermiculite (1: 1, v/v) mix, by blue powder disinfectant and life
1000 times of aqueous solutions of root powder mixed preparing, are sprayed in matrix, matrix to be irrigated, and it is ventilative to cover having for adjustable pore opening
The pallet cover of function, cuttage growing environment are sunshade, breathe freely, in the greenhouse of ventilation that control environment temperature is 26~28 DEG C,
Relative humidity is 65~80%, pours an aqueous solution week, matrix is irrigated.After culture 20 days, rooting rate 95%, no
Normal bud germination percentage 100%.
Comparison: in addition to spraying for tap water, remaining operation is same as Example 3, and after cultivating same time, rooting rate is
40%, adventitious bud germination percentage 50%.
Embodiment 4
It takes the stem section of adult Petrocosmea as explant, explant is dipped in into water of taking root (the indoles second that concentration is 0.5%
Acid solution), the oblique cuttage of the stem section is entered in the matrix that perlite and vermiculite (1: 1, v/v) mix, by blue powder disinfectant and is taken root
1000 times of aqueous solutions of powder mixed preparing, are sprayed in matrix, matrix to be irrigated, and cover adjustable pore opening has ventilative function
The pallet cover of energy, cuttage growing environment are sunshade, breathe freely, in the greenhouse of ventilation that control environment temperature is 25~28 DEG C, phase
It is 65~80% to humidity, pours an aqueous solution week, matrix is irrigated.After culture 30 days, rooting rate 90% is indefinite
Bud germination percentage 100%.
Comparison: in addition to spraying for tap water, remaining operation is same as Example 4, and after cultivating same time, rooting rate is
5%, adventitious bud germination percentage 20%.
The present invention provides a kind of cuttage breeding method of Gesneriaceae, after 25~30d of cuttage culture, rooting rate is
90%~100%, adventitious bud germination percentage is 100%, can significantly improve the rooting rate and not of Gesneriaceae explant
Normal bud germination percentage reduces the cost of Gesneriaceae large-scale production.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of cuttage breeding method of Gesneriaceae, comprising the following steps: the Gesneriaceae for water of taking root will be dipped
Mature tissue's cuttage enters matrix, cultivates 15~30d;
After the cuttage, into the matrix, every 5~9d pours a growth regulator aqueous solution;The growth regulator water
The effective component of solution includes blue powder disinfectant and root-inducing powder.
2. cuttage breeding method according to claim 1, which is characterized in that the environment of the culture is sunshade, ventilation
Greenhouse.
3. cuttage breeding method according to claim 1, which is characterized in that the Gesneriaceae mature tissue includes
Mature leaf, blade-carrying petiole or stem section.
4. cuttage breeding method according to claim 1, which is characterized in that the water of taking root be include heteroauxin and/
Or the aqueous solution of Nafusaku.
5. cuttage breeding method according to claim 1 or 4, which is characterized in that in the water of taking root heteroauxin and/or
The concentration of Nafusaku is 0.2~1%.
6. cuttage breeding method according to claim 1, which is characterized in that the mode of the cuttage includes oblique cutting.
7. cuttage breeding method according to claim 1, which is characterized in that the temperature of the culture is 18~32 DEG C.
8. cuttage breeding method according to claim 1, which is characterized in that the air humidity of the culture be 50~
95%.
9. cuttage breeding method according to claim 1, which is characterized in that in the growth regulator aqueous solution it is effective at
Divide and is diluted with water 1000 times.
10. cuttage breeding method according to claim 9, which is characterized in that the growth regulator aqueous solution it is effective at
The concentration ratio of blue powder disinfectant and root-inducing powder is 1:1 in point.
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Cited By (2)
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CN112931073A (en) * | 2021-01-27 | 2021-06-11 | 中国科学院华南植物园 | Method for naturally regressing Yaoshan moss |
CN115997632A (en) * | 2022-12-26 | 2023-04-25 | 湖南农业大学 | Method for improving tobacco planting soil characteristics and tobacco leaf quality by intercropping flue-cured tobacco and endive |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN112931073A (en) * | 2021-01-27 | 2021-06-11 | 中国科学院华南植物园 | Method for naturally regressing Yaoshan moss |
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CN115997632B (en) * | 2022-12-26 | 2024-04-19 | 湖南农业大学 | Method for improving tobacco planting soil characteristics and tobacco leaf quality by intercropping flue-cured tobacco and endive |
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Application publication date: 20181218 |