CN109005966A - A kind of cuttage breeding method of Gesneriaceae - Google Patents

A kind of cuttage breeding method of Gesneriaceae Download PDF

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Publication number
CN109005966A
CN109005966A CN201810752459.5A CN201810752459A CN109005966A CN 109005966 A CN109005966 A CN 109005966A CN 201810752459 A CN201810752459 A CN 201810752459A CN 109005966 A CN109005966 A CN 109005966A
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China
Prior art keywords
cuttage
gesneriaceae
breeding method
aqueous solution
cuttage breeding
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CN201810752459.5A
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Chinese (zh)
Inventor
邱志敬
谢锐星
罗倩
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Shenzhen City Botanical Gardens Management Office (shenzhen City Garden Research Center)
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Shenzhen City Botanical Gardens Management Office (shenzhen City Garden Research Center)
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Priority to CN201810752459.5A priority Critical patent/CN109005966A/en
Publication of CN109005966A publication Critical patent/CN109005966A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention provides a kind of cuttage breeding methods of Gesneriaceae, belong to field of plant growing technology.The method of the invention is the following steps are included: enter matrix, 15~30d of culture for the Gesneriaceae mature tissue cuttage for dipping water of taking root;After the cuttage, into the matrix, every 5~9d pours a growth regulator aqueous solution;The effective component of the growth regulator aqueous solution includes blue powder disinfectant and root-inducing powder.Utilize method of the invention, after 25~30d of cuttage culture, rooting rate is 90%~100%, and adventitious bud germination percentage is 100%, the rooting rate and adventitious bud germination percentage of Gesneriaceae explant can be significantly improved, the cost of Gesneriaceae large-scale production is reduced.

Description

A kind of cuttage breeding method of Gesneriaceae
Technical field
The invention belongs to field of plant growing technology, and in particular to a kind of cuttage breeding method of Gesneriaceae.
Background technique
Gesneriaceae (Gesneriaceae) plant about 150 belongs to 3700 kinds, is distributed widely in tropical and subtropical region, I State about 59 belongs to 493 kinds, is distributed mainly on the Changjiang river areas to the south.Section's plant flower type variation multiplicity, ornamental value height, leaf color spot It refutes, plant type rounding or shape of crawling, and shade tolerance with higher.Primulina tabacum category is excellent ornamental flower resource, most of Primulina tabacum platymiscium is alternatively arranged as medicinal;Hemiboea, Lysionotus are precious resources of medicinal plant, and lipstick flower belongs to It is emerging ornamental flower on the market, there is great value of exploiting and utilizing, Petrocosmea plant type is graceful, exquisite unique, has huge Big market developing and utilizingpotentiality.Other ornamental class Gesneriaceae such as African violet categories, gorgeous spot lettuce tongue fur category, gloxinia category Deng prevailing already on flowers market, there is certain application value in landscape application.Wherein African violet (Saintpaulia), gloxinia (Sinningia speciosa), lipstick flower (Aeschynanthuspulche) etc. are flowers Commodity potted flower extremely popular in market, and perfect merchandized handling system is had been set up, but the ornamental plant excellent to other Object germ plasm resource does not carry out commercialized development.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of Conandron ramondioides sieb. Et zuce can plant cuttage breeding method, it is easy, fast It is fast, efficient, the rooting rate and adventitious bud germination percentage of Gesneriaceae explant are significantly improved, to improve cutting propagation Success rate, improve input-output ratio.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of cuttage breeding methods of Gesneriaceae, comprising the following steps: will dip water of taking root The cuttage of Gesneriaceae mature tissue enter matrix, cultivate 15~30d;
After the cuttage, into the matrix, every 5~9d pours a growth regulator aqueous solution;The growth regulating The effective component of agent aqueous solution includes blue powder disinfectant and root-inducing powder.
Preferably, the environment of the culture is the greenhouse of sunshade, ventilation.
Preferably, the Gesneriaceae mature tissue includes mature leaf, blade-carrying petiole or stem section.
Preferably, the water of taking root is the aqueous solution for including heteroauxin and/or Nafusaku.
Preferably, the concentration of heteroauxin and/or Nafusaku is 0.2~1% in the water of taking root.
Preferably, the mode of the cuttage includes oblique cutting.
Preferably, the temperature of the culture is 18~32 DEG C.
Preferably, the air humidity of the culture is 50~95%.
Preferably, the effective component in the growth regulator aqueous solution is diluted with water 1000 times.
Preferably, the concentration of blue powder disinfectant and root-inducing powder ratio is 1 in the effective component of the growth regulator aqueous solution: 1。
The present invention provides a kind of cuttage breeding methods of Gesneriaceae, are outer with Gesneriaceae mature tissue Implant carries out cuttage, and the rooting rate and adventitious bud germination percentage of Gesneriaceae explant can be improved, meanwhile, the Conandron ramondioides sieb. Et zuce of preparation Section's plant seedlings high survival rate, it is easy to operate, production cost is reduced, facilitates Gesneriaceae and large-scale develops and utilizes.This In inventive embodiments, after cuttage culture 25~30 days, rooting rate is 90~100%, adventitious bud germination percentage 100%.
Specific embodiment
The present invention provides a kind of cuttage breeding methods of Gesneriaceae, comprising the following steps: will dip water of taking root The cuttage of Gesneriaceae mature tissue enter matrix, cultivate 15~30d;
After the cuttage, into the matrix, every 5~9d pours a growth regulator aqueous solution;The growth regulating The effective component of agent aqueous solution includes blue powder disinfectant and root-inducing powder.
In the cuttage breeding method of Gesneriaceae of the present invention, the Gesneriaceae for dipping water of taking root is planted first The cuttage of object mature tissue enters matrix.Gesneriaceae mature tissue of the present invention preferably includes mature leaf, with blade Petiole or stem section.There is no particular determinations in source of the present invention to the Gesneriaceae mature tissue, are preferably derived from Robust growth, disease-free maternal plant.When using blade-carrying petiole as explant, the present invention is preferably directly cut on maternal plant Petiole base is taken, it is described to cut off part leaf margin, to reduce transpiration;When the present invention is to using mature leaf or stem section as explant There is no particular determinations for acquisition modes.
The explant is dipped water of taking root by the present invention, and the effective component of the water of taking root preferably comprises heteroauxin And/or Nafusaku, more preferably heteroauxin or Nafusaku, most preferably heteroauxin.In water of the present invention of taking root The concentration of effective component is preferably 0.2%~1%, and more preferably 0.3%~0.8%, most preferably 0.5%.The present invention is to institute Stating the mode that dips of water of taking root, there is no particular determinations, and the explant is preferably dipped in 3~10s in the water of taking root.
The present invention enters the explant cuttage for dipping water of taking root in matrix, and the mode of the cuttage preferably includes oblique cutting.This Invent the mixture that the matrix preferably comprises perlite and vermiculite, the volume ratio of the perlite and vermiculite is preferably 1: (0.6~2), more preferably 1:(0.8~1.5), most preferably 1:1.The matrix is preferably filled in hole tray by the present invention, institute Stating hole tray is preferably 36 hole hole trays.When the present invention carries out cuttage as explant using mature leaf, when leaf area is larger, such as tobacco leaf report Spring lettuce tongue fur, Guilin primulina tabacum etc. form wound preferably at vein, and blade are tiled in matrix, cover institute in wound State matrix.The quantity of wound described in every leaf of the present invention is preferably 1~2.The present invention to the forming method of the wound not There is particular determination, preferably breaks and disconnected form wound.
The present invention cultivates 15~30d, the environment of the culture is preferably the greenhouse of sunshade, ventilation after cuttage.? In the present invention, the temperature of the culture is preferably 18~32 DEG C, more preferably 24~29 DEG C, most preferably 25~28 DEG C;It is described The relative humidity of culture is preferably 50~95%, and more preferably 60~85%, most preferably 65~80%.
The present invention is after the cuttage, and into the matrix, every 5~9d pours a growth regulator aqueous solution;The life The effective component of long regulator aqueous solution includes blue powder disinfectant and root-inducing powder.It is blue in growth regulator aqueous solution of the present invention The concentration of powder disinfectant and root-inducing powder is than being preferably 1:(0.6~1.5), more preferably 1:(0.8~1.2), most preferably 1:1, When in use, the concentration of blue powder disinfectant and root-inducing powder is 1000 times of aqueous solutions in the growth regulator aqueous solution.This hair There is no particular determinations in bright source and type to blue powder disinfectant and root-inducing powder in the growth regulator aqueous solution.The present invention The amount of spraying of the growth regulator aqueous solution preferably irrigates matrix.Growth regulator aqueous solution of the present invention sprays frequency Rate is preferably 6~8d primary, and more preferably 7d is primary.
The present invention preferably further includes the process of anti-moisture evaporation after spraying the growth regulator aqueous solution, described Preferably by plastic covering lid, the plastic lid is preferably the pallet cover with vent valve for anti-moisture evaporation, and it is big that stomata is adjusted It is small, prevent moisture from evaporating.
Present invention 15d or so after the cuttage, that is, can produce callus, and differentiate adventitious root and bud, formed Whole plant.
It is described in detail below with reference to cuttage breeding method of the embodiment to Gesneriaceae provided by the invention, But they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
Take adult petiole base of the primulina tabacum category with blade as explant, explant is dipped in water of taking root, and (concentration is 0.5% heteroauxin solution), the oblique cuttage of the petiole is entered into the matrix that perlite and vermiculite (1: 1, v/v) mix and (is loaded on 36 holes In hole tray) in, blade is stood on matrix face, and explant cuttage is after matrix, by blue powder disinfectant and root-inducing powder mixed preparing 1000 times of aqueous solutions are sprayed in 36 hole plug medias, matrix to be irrigated, and cover adjustable pore opening has airing function Pallet cover, cuttage growing environment is sunshade, in ventilative, ventilation greenhouse, and control environment temperature is 26~28 DEG C, relatively Humidity is 65~80%, pours an aqueous solution week, matrix is irrigated.After culture 30 days, rooting rate 100% is indefinite Bud germination percentage 100%.
Comparison: in addition to spraying for tap water, remaining operation is same as Example 1, and after cultivating same time, rooting rate is 30%, adventitious bud germination percentage 50%.
Embodiment 2
It takes the blade of adult gloxinia as explant, explant is dipped in into water of the taking root (heteroauxin that concentration is 0.5% Solution), the oblique cuttage of the blade is entered in the matrix that perlite and vermiculite (1: 1, v/v) mix, by blue powder disinfectant and root-inducing powder 1000 times of aqueous solutions of mixed preparing, are sprayed in matrix, matrix to be irrigated, and cover adjustable pore opening has airing function Pallet cover, cuttage growing environment is sunshade, in ventilative, ventilation greenhouse, and control environment temperature is 26~28 DEG C, relatively Humidity is 65~80%, pours an aqueous solution week, matrix is irrigated.After culture 25 days, rooting rate 100% is indefinite Bud germination percentage 100%.
Comparison: in addition to spraying for tap water, remaining operation is same as Example 2, and after cultivating same time, rooting rate is 20%, adventitious bud germination percentage 30%.
Embodiment 3
It takes the stem section of adult Hemiboea as explant, explant is dipped in into water of the taking root (indoles that concentration is 0.5% Acetic acid solution), the oblique cuttage of the stem section is entered in the matrix that perlite and vermiculite (1: 1, v/v) mix, by blue powder disinfectant and life 1000 times of aqueous solutions of root powder mixed preparing, are sprayed in matrix, matrix to be irrigated, and it is ventilative to cover having for adjustable pore opening The pallet cover of function, cuttage growing environment are sunshade, breathe freely, in the greenhouse of ventilation that control environment temperature is 26~28 DEG C, Relative humidity is 65~80%, pours an aqueous solution week, matrix is irrigated.After culture 20 days, rooting rate 95%, no Normal bud germination percentage 100%.
Comparison: in addition to spraying for tap water, remaining operation is same as Example 3, and after cultivating same time, rooting rate is 40%, adventitious bud germination percentage 50%.
Embodiment 4
It takes the stem section of adult Petrocosmea as explant, explant is dipped in into water of taking root (the indoles second that concentration is 0.5% Acid solution), the oblique cuttage of the stem section is entered in the matrix that perlite and vermiculite (1: 1, v/v) mix, by blue powder disinfectant and is taken root 1000 times of aqueous solutions of powder mixed preparing, are sprayed in matrix, matrix to be irrigated, and cover adjustable pore opening has ventilative function The pallet cover of energy, cuttage growing environment are sunshade, breathe freely, in the greenhouse of ventilation that control environment temperature is 25~28 DEG C, phase It is 65~80% to humidity, pours an aqueous solution week, matrix is irrigated.After culture 30 days, rooting rate 90% is indefinite Bud germination percentage 100%.
Comparison: in addition to spraying for tap water, remaining operation is same as Example 4, and after cultivating same time, rooting rate is 5%, adventitious bud germination percentage 20%.
The present invention provides a kind of cuttage breeding method of Gesneriaceae, after 25~30d of cuttage culture, rooting rate is 90%~100%, adventitious bud germination percentage is 100%, can significantly improve the rooting rate and not of Gesneriaceae explant Normal bud germination percentage reduces the cost of Gesneriaceae large-scale production.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered It is considered as protection scope of the present invention.

Claims (10)

1. a kind of cuttage breeding method of Gesneriaceae, comprising the following steps: the Gesneriaceae for water of taking root will be dipped Mature tissue's cuttage enters matrix, cultivates 15~30d;
After the cuttage, into the matrix, every 5~9d pours a growth regulator aqueous solution;The growth regulator water The effective component of solution includes blue powder disinfectant and root-inducing powder.
2. cuttage breeding method according to claim 1, which is characterized in that the environment of the culture is sunshade, ventilation Greenhouse.
3. cuttage breeding method according to claim 1, which is characterized in that the Gesneriaceae mature tissue includes Mature leaf, blade-carrying petiole or stem section.
4. cuttage breeding method according to claim 1, which is characterized in that the water of taking root be include heteroauxin and/ Or the aqueous solution of Nafusaku.
5. cuttage breeding method according to claim 1 or 4, which is characterized in that in the water of taking root heteroauxin and/or The concentration of Nafusaku is 0.2~1%.
6. cuttage breeding method according to claim 1, which is characterized in that the mode of the cuttage includes oblique cutting.
7. cuttage breeding method according to claim 1, which is characterized in that the temperature of the culture is 18~32 DEG C.
8. cuttage breeding method according to claim 1, which is characterized in that the air humidity of the culture be 50~ 95%.
9. cuttage breeding method according to claim 1, which is characterized in that in the growth regulator aqueous solution it is effective at Divide and is diluted with water 1000 times.
10. cuttage breeding method according to claim 9, which is characterized in that the growth regulator aqueous solution it is effective at The concentration ratio of blue powder disinfectant and root-inducing powder is 1:1 in point.
CN201810752459.5A 2018-07-10 2018-07-10 A kind of cuttage breeding method of Gesneriaceae Pending CN109005966A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112931073A (en) * 2021-01-27 2021-06-11 中国科学院华南植物园 Method for naturally regressing Yaoshan moss
CN115997632A (en) * 2022-12-26 2023-04-25 湖南农业大学 Method for improving tobacco planting soil characteristics and tobacco leaf quality by intercropping flue-cured tobacco and endive

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011244705A (en) * 2010-05-24 2011-12-08 Idemitsu Kosan Co Ltd Method for cultivating plant
CN102405755A (en) * 2011-09-13 2012-04-11 中国科学院华南植物园 Method for breeding adventitious buds and adventitious roots by cuttage induction of primulina tabacum hance petioles
CN103947524A (en) * 2014-04-24 2014-07-30 中国科学院华南植物园 Breeding method for inducing adventitious buds and roots by cutting petioles of metabriggsia ovalifolia
CN104255272A (en) * 2014-10-14 2015-01-07 南京帝道农业科技有限公司 Rapid propagation method for root cutting of lysionotus pauciflorus
CN106550762A (en) * 2016-12-07 2017-04-05 贵州省林业科学研究院 A kind of Libo lip post lettuce tongue cuttage breeding method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011244705A (en) * 2010-05-24 2011-12-08 Idemitsu Kosan Co Ltd Method for cultivating plant
CN102405755A (en) * 2011-09-13 2012-04-11 中国科学院华南植物园 Method for breeding adventitious buds and adventitious roots by cuttage induction of primulina tabacum hance petioles
CN103947524A (en) * 2014-04-24 2014-07-30 中国科学院华南植物园 Breeding method for inducing adventitious buds and roots by cutting petioles of metabriggsia ovalifolia
CN104255272A (en) * 2014-10-14 2015-01-07 南京帝道农业科技有限公司 Rapid propagation method for root cutting of lysionotus pauciflorus
CN106550762A (en) * 2016-12-07 2017-04-05 贵州省林业科学研究院 A kind of Libo lip post lettuce tongue cuttage breeding method

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
李振宇等: "《中国苦苣苔科植物》", 31 January 2005, 河南科学技术出版社 *
章文才: "《果树研究法 第3版》", 31 May 1980, 中国农业出版社 *
赵日成: "长瓣马铃苣苔(Oreocharis auricula)的扦插繁殖技术和抗逆性研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 *
金雪琼等: "崀山唇柱苣苔的快速繁殖", 《植物生理学通讯》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112931073A (en) * 2021-01-27 2021-06-11 中国科学院华南植物园 Method for naturally regressing Yaoshan moss
CN115997632A (en) * 2022-12-26 2023-04-25 湖南农业大学 Method for improving tobacco planting soil characteristics and tobacco leaf quality by intercropping flue-cured tobacco and endive
CN115997632B (en) * 2022-12-26 2024-04-19 湖南农业大学 Method for improving tobacco planting soil characteristics and tobacco leaf quality by intercropping flue-cured tobacco and endive

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Application publication date: 20181218