CN108901942A - A kind of method of Ba Shayu seedling artificial propagation - Google Patents

A kind of method of Ba Shayu seedling artificial propagation Download PDF

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Publication number
CN108901942A
CN108901942A CN201810659502.3A CN201810659502A CN108901942A CN 108901942 A CN108901942 A CN 108901942A CN 201810659502 A CN201810659502 A CN 201810659502A CN 108901942 A CN108901942 A CN 108901942A
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China
Prior art keywords
seedling
cultivating
shayu
water
hatching
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CN201810659502.3A
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Inventor
骆明飞
李江华
盘润洪
于方兆
邓樱
曾晓辉
刘湘
李勇
郭建谊
陈创华
李望东
罗志平
古群红
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Zhuhai Dayue Agricultural And Fisheries Technology Co Ltd
Zhuhai modern agricultural development center
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Zhuhai Dayue Agricultural And Fisheries Technology Co Ltd
Zhuhai modern agricultural development center
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Priority to CN201810659502.3A priority Critical patent/CN108901942A/en
Publication of CN108901942A publication Critical patent/CN108901942A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • A01K61/17Hatching, e.g. incubators
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a kind of methods of Ba Shayu seedling artificial propagation, this propagation method uses the cultivating system of controllable type, the ingenious outer circulation water system using formation such as cultivating workshop, cultivating pool and various common breeding facilities carries out artificial breeding, fine water needed for ensure that cultivation, survival rate is improved, provides direction for standardization nursery;Furthermore, Ba Shayu can be realized artificial propagation by this cultural method, and seedling raise period can shift to an earlier date 1-2 months in relatively present breeding method, it is particluarly suitable for South China of China and carries out pre-incubation, extend the cultivation season of Ba Shayu, condition is improved for cultivation of avoiding the peak hour, sale, Ba Shayu culture efficiency is greatly improved.

Description

A kind of method of Ba Shayu seedling artificial propagation
Technical field
The present invention relates to Ba Shayu cultural technique field more particularly to a kind of methods of Ba Shayu seedling artificial propagation.
Background technique
Ba Shayu is the important freshwater aquiculture kind of country in Southeast Asia.It belongs to Silurformes fish, belongs to the awns Nian that awns Nian belongs to and is Alepidote class is a kind of distinctive Quality and economy fish in the Mekong River valley.The fish is fast with growth, individual is big, yield height, food Property it is wide, easily raising, meat be delicate, delicious flavour, it is full of nutrition, without spinule between flesh, be conducive to the advantages that processing.Current year, seed was through one Year raising can be grown to 500-800 grams, and next year can grow to 1500-2000 grams, and maximum individual is up to 15 kilograms.
Ba Shayu started to introduce South China cultivation in 2009, but mainly by cultivation weather, seed breeding, feed Etc. the influences of factors fail to be widely applied.Ba Shayu is tropic fishes, and suitable growth water temperature is 20 DEG C~32 DEG C, optimum water temperature It is 24 DEG C~30 DEG C, when water temperature is down to 16 DEG C, Ba Shayu stops feed, and water temperature is at 10 DEG C hereinafter, then can frostbite or even death. Therefore, in South China, it is suitble to the season of Ba Sha fish culture to be generally the 5-10 month, traditional modes of reproduction is be easy to cause The problems such as breeding, cultivation and central selling, is concentrated in transition, influences the industry development of Ba Shayu.
Summary of the invention
In order to overcome the above-mentioned deficiencies of the prior art, the present invention provides a kind of using controllable artificial propagation mode, no The growth characteristics of Ba Shayu are suitable only for, also climate environment does not influence, and has many advantages, such as controllability, low cost, water-saving, efficient.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of method of Ba Shayu seedling artificial propagation, includes the following steps:
The preparation of step 1, breeding facility:It is ready for temporarily supporting and/or cultivating the cultivating workshop of parent population and seed, use In the hatching barrel of hatching fry and for carrying out marking thick cultivating pool;
Step 2, parent fish rearing:Select figure is normal, disease-free, energetic, sexal maturity preferably and 4 age of raun with On, the adult fish more than age of milter 5 is as parent population;In mid or late January, 25 DEG C~30 DEG C of water temperature, dissolved oxygen are in cultivating workshop Cultivation parent population 2 months, cultivation density 2-3 tail/m are carried out in the environment of 4mg/L or more2,, and in the morning at night feed adding Parent population feed added with vitamin, containing high protein, each feeding volume are the 2%~3% of parent population weight;
Step 3, artificial induced spawning:In mid or late March, selects physical strong mature brood fish and hasten parturition, female-male proportion 3: 1;Between 25 DEG C~30 DEG C, using dry method of artificial fertilization, male and female point pond is temporarily supported in parent population after hastening parturition, and is held for water temperature control when hastening parturition Continuous oxygenation, and stimulation parent population is carried out with certain continuous flowing water;
Step 4, hatching fry:Unsticking is carried out to the fertilized eggs hastened parturition using yellow mud or talcum powder, utilizes clear water It after rinsing fertilized eggs and pours into hatching barrel and is hatched, hatching water temperature is consistent with the water temperature hastened parturition, and hatching 20-22h can hatch Film completes hatching;
Step 5, seedling culture:The seedling that hatching is completed is transferred to cultivating workshop temporarily to be supported;Yellow capsule to seedling becomes After small, seedling enters opening breeding phase, and controlling cultivation density at this time is ten thousand tails of 3-4/m3, and the gradually decreased density in one week To ten thousand tails of 1-1.5/m3, food organisms during which and are in time fed, keep cultivating water temperature between 25 DEG C~30 DEG C, dissolved oxygen 4mg/ L or more.
As an improvement of the above technical solution, the concrete operations of step 1 are:
Cultivating workshop is built, and building area is 8-30m2Between cement pit, in cement pit be arranged discharge pipe line, plus Pipe line and oxygen increasing equipment;
The setting of hatching barrel, selects volume for 0.9-1.2m3, the tapered plastic barrel in bottom is provided in the plastic barrel Oxygenation pipeline and the water coke slurry device that water coke slurry can be carried out with the external world;
Cultivating pool is built, and excavates precipitating pond and biological treatment pond, the two face in the region close to cultivating workshop Product size is 1:1, and be connected to by pipeline, the total water body for precipitating pond and biological treatment pond is cultivating workshop water 5 times or more of body.
As an improvement of the above technical solution, in the build process of cultivating pool, biological treatment pond is by water pump and supports Workshop connection is grown, cultivating workshop can be connected to by pipeline with precipitating pond.
As an improvement of the above technical solution, the concrete operations of step 3 are:
Step 3.1, in mid or late March, select physical strong mature brood fish and hasten parturition, female-male proportion 3: 1, the company of offer Continuous water flow stimulates parent population;
Step 3.2 carries out injection oxytocic drug to raun and milter, wherein oxytocic drug be dioxone (DOM), HCG and LRH-A2;
Step 3.3 injects twice raun, injects LRH-A2 for the first time, and dosage is 10~15 μ g of per kilogram raun, The second needle is injected after having injected the first needle 10-12h, second of injection uses HCG800IU, LRH-A210~15ug and DOM8 The mixture of~10mg;While raun injects the second needle, the mixture halved to dosage is injected to milter;
Step 3.4, using dry method of artificial fertilization, squeeze the gonopore of raun, obtain ovum and be placed on a clean appearance In device, the gonopore for squeezing milter obtains sperm, and sperm is mixed with ovum, wherein the sperm of a milter and 3-5 raun Ovum mixed, be added with ovum quality ratio be 1:1 physiological saline carries out mixing sperm and ovum, incorporation time are 20-30s;
Step 3.5 after hastening parturition, carries out point pond to male female parent population and temporarily supports, and water temperature is 25 DEG C~30 DEG C, increases dissolved oxygen in water Amount, increased water flow stimulate parent population.
As an improvement of the above technical solution, step 4 further includes the following contents, and when hatching, hatching barrel is passed through constant flow Flowing water, in hatching barrel water body carry out oxygenation, control oxygenation amount and flowing water speed cooperation so that fish-egg can be in hatching barrel Interior soft rolling, and keep not sinking to hatching barrel bottom.
As an improvement of the above technical solution, step 5 the specific steps are:
Step 5.1, after fish-egg whole rupture of membranes is hatched into seedling body, be transferred to cultivating workshop with the suction of soft siphon pipe and carried out temporarily It supports, temporarily feeding water temperature is between 25 DEG C~30 DEG C, and dissolved oxygen is 4mg/L or more;
Step 5.2, membrane 2-3 days, after the yellow capsule of seedling becomes smaller, seedling enters opening breeding phase, at this time control cultivation Density is ten thousand tails of 3-4/m3, food organisms are fed in time, feed 5-10/ml of food density;
Step 5.3, membrane 4-7 days need to increase and feed food organisms, feed 8-10/ml of food density, and reduce cultivation Density is to ten thousand tails of 2-2.5/m3, keep cultivating 25 DEG C of water temperature or more, inflation keeps dissolved oxygen 4mg/l or more;
Gradually transition feeds artifical compound feed after a week for step 5.4, membrane, can feed hand fit completely within 10-12 days Feed, feeding volume account for fish body weight 5-8%;It keeps cultivating 25 DEG C of water temperature or more, inflation keeps dissolved oxygen 4mg/l or more.
As an improvement of the above technical solution, the food organisms be fairy shrimp nauplius, wheel animalcule, small-sized cladocera and The one or more of water earthworm.
As an improvement of the above technical solution, further comprising the steps of after step 5:Step 6, seedling cultivate 14-17 days Later, when fry growth to specification is 2.5-3.1cm, fry is transferred in ordinary pond and is cultivated.
Beneficial effects of the present invention have:
Ba Shayu can be realized artificial propagation by this propagation method, and seedling raise period can be mentioned in relatively present breeding method Preceding 1-2 months, it is particluarly suitable for South China of China and carries out pre-incubation, extend the cultivation season of Ba Shayu, it is feeding to avoid the peak hour It grows, sell the condition of improving, Ba Shayu culture efficiency is greatly improved;This cultural method uses the cultivating system of controllable type, ingenious Carry out artificial breeding using the outer circulation water system of the formation such as cultivating workshop, cultivating pool and various common breeding facilities, protects Fine water needed for having demonstrate,proved cultivation, improves survival rate, provides direction for standardization nursery.
Specific embodiment
A kind of method of Ba Shayu seedling artificial propagation of the invention, includes the following steps:
The preparation of step 1, breeding facility:It is ready for temporarily supporting and/or cultivating the cultivating workshop of parent population and seed, use In the hatching barrel of hatching fry and for carrying out marking thick cultivating pool;
Step 2, parent fish rearing:Select figure is normal, disease-free, energetic, sexal maturity preferably and 4 age of raun with On, the adult fish more than age of milter 5 is as parent population;In mid or late January, 25 DEG C~30 DEG C of water temperature, dissolved oxygen are in cultivating workshop Cultivation parent population 2 months, cultivation density 2-3 tail/m are carried out in the environment of 4mg/L or more2,, and in the morning at night feed adding Parent population feed added with vitamin, containing high protein, each feeding volume are the 2%~3% of parent population weight;
Step 3, artificial induced spawning:In mid or late March, selects physical strong mature brood fish and hasten parturition, female-male proportion 3: 1;Between 25 DEG C~30 DEG C, using dry method of artificial fertilization, male and female point pond is temporarily supported in parent population after hastening parturition, and is held for water temperature control when hastening parturition Continuous oxygenation, and stimulation parent population is carried out with certain continuous flowing water;
Step 4, hatching fry:Unsticking is carried out to the fertilized eggs hastened parturition using yellow mud or talcum powder, utilizes clear water It after rinsing fertilized eggs and pours into hatching barrel and is hatched, hatching water temperature is consistent with the water temperature hastened parturition, and hatching 20-22h can hatch Film completes hatching;
Step 5, seedling culture:The seedling that hatching is completed is transferred to cultivating workshop temporarily to be supported;Yellow capsule to seedling becomes After small, seedling enters opening breeding phase, and controlling cultivation density at this time is ten thousand tails of 3-4/m3, and the gradually decreased density in one week To ten thousand tails of 1-1.5/m3, food organisms during which and are in time fed, keep cultivating water temperature between 25 DEG C~30 DEG C, dissolved oxygen 4mg/ L or more.
Ba Shayu can be realized artificial propagation by this propagation method, and seedling raise period can be mentioned in relatively present breeding method Preceding 1-2 months, it is particluarly suitable for South China of China and carries out pre-incubation, extend the cultivation season of Ba Shayu, it is feeding to avoid the peak hour It grows, sell the condition of improving, Ba Shayu culture efficiency is greatly improved;This cultural method uses the cultivating system of controllable type, ingenious Carry out artificial breeding using the outer circulation water system of the formation such as cultivating workshop, cultivating pool and various common breeding facilities, protects Fine water needed for having demonstrate,proved cultivation, improves survival rate, provides direction for standardization nursery.
In the present invention, the concrete operations of step 1 are:
Cultivating workshop is built, and building area is 8-30m2Between cement pit, in cement pit be arranged discharge pipe line, plus Pipe line and oxygen increasing equipment;
The setting of hatching barrel, selects volume for 0.9-1.2m3, the tapered plastic barrel in bottom is provided in the plastic barrel Oxygenation pipeline and the water coke slurry device that water coke slurry can be carried out with the external world;
Cultivating pool is built, and excavates precipitating pond and biological treatment pond, the two face in the region close to cultivating workshop Product size is 1:1, and be connected to by pipeline, the total water body for precipitating pond and biological treatment pond is cultivating workshop water 5 times or more of body.
In the build process of cultivating pool, biological treatment pond is connected to by water pump with cultivating workshop, and cultivating workshop passes through Pipeline can be connected to precipitating pond.The water quality that processing cultivating workshop can be carried out using precipitating pond, i.e., used cultivating workshop The water quality crossed carries out sediment miscellaneous object, reduces the microorganism in water quality, and the disease for reducing parent population either seedling occurs;Utilize biology The water quality precipitated can be effectively treated in processing pond, the water body in sedimentation basin can be carried out microbiological treatment, carry out rich water It either sterilizes, facilitates and utilized.The application mini-bus shark artificial culture seedling that is set as of entire cultivating pool provides Good water body directly affects surviving for seedling.
In addition, the concrete operations of step 3 are in the present invention:
Step 3.1, in mid or late March, select physical strong mature brood fish and hasten parturition, female-male proportion 3: 1, the company of offer Continuous water flow stimulates parent population;
Step 3.2 carries out injection oxytocic drug to raun and milter, wherein oxytocic drug be dioxone (DOM), HCG and LRH-A2;
Step 3.3 injects twice raun, injects LRH-A2 for the first time, and dosage is 10~15 μ g of per kilogram raun, The second needle is injected after having injected the first needle 10-12h, second of injection uses HCG800IU, LRH-A210~15ug and DOM8 The mixture of~10mg;While raun injects the second needle, the mixture halved to dosage is injected to milter;
Step 3.4, using dry method of artificial fertilization, squeeze the gonopore of raun, obtain ovum and be placed on a clean appearance In device, the gonopore for squeezing milter obtains sperm, and sperm is mixed with ovum, wherein the sperm of a milter and 3-5 raun Ovum mixed, be added with ovum quality ratio be 1:1 physiological saline carries out mixing sperm and ovum, incorporation time are 20-30s;
Step 3.5 after hastening parturition, carries out point pond to male female parent population and temporarily supports, and water temperature is 25 DEG C~30 DEG C, increases dissolved oxygen in water Amount, increased water flow stimulate parent population.
In above-mentioned steps 3.4, the operation specifically squeezed is that a people embraces fish, and the upward tail of head is downward and pins reproduction with hand Hole, can be to avoid in order to avoid ovum flows in water, and another people then uses hand soft with holding caudal peduncle and being dried fish body abdomen with towel Ground squeezes abdomen, and using first rear portion, the mode of rear front is squeezed.In step 3.5, increased water flow stimulates parent population, Ke Yiyou Effect restores parent population.
In the present invention, step 4 further includes the following contents, and when hatching, hatching barrel is passed through the flowing water of constant flow, to hatching barrel Interior water body carries out oxygenation, controls the cooperation of oxygenation amount and flowing water speed, so that fish-egg can softly roll in hatching barrel, and protects It holds and does not sink to hatching barrel bottom.Fish-egg can be effectively prevented in this manner there is the case where anoxic is suffocated It is existing, while the breeding situation under wild environment can also be simulated, improve survival rate.
In the present invention, step 5 the specific steps are:
Step 5.1, after fish-egg whole rupture of membranes is hatched into seedling body, be transferred to cultivating workshop with the suction of soft siphon pipe and carried out temporarily It supports, temporarily feeding water temperature is between 25 DEG C~30 DEG C, and dissolved oxygen is 4mg/L or more;
Step 5.2, membrane 2-3 days, after the yellow capsule of seedling becomes smaller, seedling enters opening breeding phase, at this time control cultivation Density is ten thousand tails of 3-4/m3, food organisms are fed in time, feed 5-10/ml of food density;
Step 5.3, membrane 4-7 days need to increase and feed food organisms, feed 8-10/ml of food density, and reduce cultivation Density is to ten thousand tails of 2-2.5/m3, keep cultivating 25 DEG C of water temperature or more, inflation keeps dissolved oxygen 4mg/l or more;
Gradually transition feeds artifical compound feed after a week for step 5.4, membrane, can feed hand fit completely within 10-12 days Feed, feeding volume account for fish body weight 5-8%;It keeps cultivating 25 DEG C of water temperature or more, inflation keeps dissolved oxygen 4mg/l or more.
5.3 further supplement through the above steps can both prevent fry in opening breeding phase because very fastly raw It is long and cause mutually to kill and devour, avoid the fry death rate from increasing, albumen needed for feeding biological feed early period and both can guaranteeing seedling Matter and nutriment, moreover it is possible to improve the vigor of seedling predation.Children can more be improved compared to existing feed by wherein feeding biological feed The survival rate of seedling.
In the present invention, the food organisms be fairy shrimp nauplius, wheel animalcule, small-sized cladocera and water earthworm one kind Or it is a variety of.These food organisms are common biology, are easy to obtain, cultivate at low cost.
In addition, further comprising the steps of after step 5:After step 6, seedling cultivate 14-17 days, when fry growth to rule When lattice are 2.5-3.1cm, fry is transferred in ordinary pond and is cultivated.Since fry growth has reached the specification, then Cultivated using cultivating pool, aquaculture cost will be gradually increased, in addition, fry under the specification, carry out transfer fry or In packaging process, the vigor of fry is relatively high, moves high survival rate behind the pool.
The above, only better embodiment of the invention, but the present invention is not limited to above-described embodiments, as long as The technical effect of the present invention is achieved by any identical or similar means for it, all should belong to protection scope of the present invention.

Claims (8)

1. a kind of method of Ba Shayu seedling artificial propagation, which is characterized in that include the following steps:
The preparation of step 1, breeding facility:It is ready for temporarily supporting and/or cultivating the cultivating workshop of parent population and seed, for incubating Change the hatching barrel of fry and for carrying out marking thick cultivating pool;
Step 2, parent fish rearing:Selection figure is normal, disease-free, energetic, sexal maturity is preferable and raun 4 is more than age, male The adult fish more than age of fish 5 is as parent population;In mid or late January, 25 DEG C~30 DEG C of water temperature in cultivating workshop, dissolved oxygen be 4mg/L with On environment in carry out cultivation parent population 2 months, cultivation density 2-3 tail/m2,, and in the morning with carry out feeding at night added with dimension life Element, the parent population feed containing high protein, each feeding volume are the 2%~3% of parent population weight;
Step 3, artificial induced spawning:In mid or late March, selects physical strong mature brood fish and hasten parturition, female-male proportion 3: 1;It urges Water temperature control is between 25 DEG C~30 DEG C when production, and using dry method of artificial fertilization, male and female point pond is temporarily supported in parent population after hastening parturition, lasting to increase Oxygen, and stimulation parent population is carried out with certain continuous flowing water;
Step 4, hatching fry:Unsticking is carried out to the fertilized eggs hastened parturition using yellow mud or talcum powder, is rinsed using clear water After fertilized eggs and pour into hatching barrel and hatched, hatching water temperature is consistent with the water temperature hastened parturition, hatch 20-22h can incubation of membrane, it is complete At hatching;
Step 5, seedling culture:The seedling that hatching is completed is transferred to cultivating workshop temporarily to be supported;After the yellow capsule of seedling becomes smaller, Seedling enters opening breeding phase, and controlling cultivation density at this time is ten thousand tails of 3-4/m3, and in one week gradually decreased density to 1- 1.5 ten thousand tails/m3, during which and in time feed food organisms, keep cultivating water temperature between 25 DEG C~30 DEG C, dissolved oxygen be 4mg/L with On.
2. a kind of method of Ba Shayu seedling artificial propagation according to claim 1, which is characterized in that the specific behaviour of step 1 As:
Cultivating workshop is built, and building area is 8-30m2Between cement pit, in cement pit be arranged discharge pipe line, heating tube Road and oxygen increasing equipment;
The setting of hatching barrel, selects volume for 0.9-1.2m3, the tapered plastic barrel in bottom is provided with oxygen increasing tube in the plastic barrel Road and the water coke slurry device that water coke slurry can be carried out with the external world;
Cultivating pool is built, and excavates precipitating pond in the region close to cultivating workshop and biological treatment pond, the two area are big Small scale is 1:1, and be connected to by pipeline, the total water body for precipitating pond and biological treatment pond is cultivating workshop water body 5 times or more.
3. a kind of method of Ba Shayu seedling artificial propagation according to claim 2, which is characterized in that cultivating pool is built In the process, biological treatment pond is connected to by water pump with cultivating workshop, and cultivating workshop can be connected to by pipeline with precipitating pond.
4. a kind of method of Ba Shayu seedling artificial propagation according to claim 1, which is characterized in that the specific behaviour of step 3 As:
Step 3.1, in mid or late March, select physical strong mature brood fish and hasten parturition, female-male proportion 3: 1 provides continuous water Stream stimulation parent population;
Step 3.2 carries out injection oxytocic drug to raun and milter, and wherein oxytocic drug is dioxone (DOM), HCG and LRH- A2;
Step 3.3 injects twice raun, injects LRH-A2 for the first time, and dosage is 10~15 μ g of per kilogram raun, is infusing Inject the second needle after shooting away the first needle 10-12h, second injection using HCG800IU, LRH-A210~15ug and DOM8~ The mixture of 10mg;While raun injects the second needle, the mixture halved to dosage is injected to milter;
Step 3.4, using dry method of artificial fertilization, squeeze the gonopore of raun, obtain ovum and be placed in a clean container, The gonopore for squeezing milter obtains sperm, sperm is mixed with ovum, wherein the ovum of the sperm of a milter and 3-5 raun It is mixed, being added with ovum quality ratio is 1:1 physiological saline carries out mixing sperm and ovum, incorporation time 20-30s;
Step 3.5 after hastening parturition, carries out point pond to male female parent population and temporarily supports, and water temperature is 25 DEG C~30 DEG C, increases dissolved oxygen in water, adds Mass-flow stimulates parent population.
5. a kind of method of Ba Shayu seedling artificial propagation according to claim 1, which is characterized in that step 4 further include with Lower content, when hatching, hatching barrel is passed through the flowing water of constant flow, carries out oxygenation to the water body in hatching barrel, control oxygenation amount and The cooperation of flowing water speed so that fish-egg can softly roll in hatching barrel, and keeps not sinking to hatching barrel bottom.
6. a kind of method of Ba Shayu seedling artificial propagation according to claim 1, which is characterized in that the specific step of step 5 Suddenly it is:
Step 5.1, after fish-egg whole rupture of membranes is hatched into seedling body, be transferred to cultivating workshop with the suction of soft siphon pipe and temporarily supported, Temporarily feeding water temperature is between 25 DEG C~30 DEG C, and dissolved oxygen is 4mg/L or more;
Step 5.2, membrane 2-3 days, after the yellow capsule of seedling becomes smaller, seedling enters opening breeding phase, controls cultivation density at this time For ten thousand tails of 3-4/m3, food organisms are fed in time, feed 5-10/ml of food density;
Step 5.3, membrane 4-7 days need to increase and feed food organisms, feed 8-10/ml of food density, and reduce cultivation density To ten thousand tails of 2-2.5/m3, keep cultivating 25 DEG C of water temperature or more, inflation keeps dissolved oxygen 4mg/l or more;
Gradually transition feeds artifical compound feed after a week for step 5.4, membrane, can feed artifical compound feed completely within 10-12 days, Feeding volume accounts for fish body weight 5-8%;It keeps cultivating 25 DEG C of water temperature or more, inflation keeps dissolved oxygen 4mg/l or more.
7. a kind of method of Ba Shayu seedling artificial propagation according to claim 1 or 6, which is characterized in that the bait is raw Object be fairy shrimp nauplius, wheel animalcule, small-sized cladocera and water earthworm one or more.
8. a kind of method of Ba Shayu seedling artificial propagation according to claim 7, which is characterized in that also wrapped after step 5 Include following steps:
After step 6, seedling cultivate 14-17 days, when fry growth to specification is 2.5-3.1cm, fry is transferred to commonly It is cultivated in pond.
CN201810659502.3A 2018-06-25 2018-06-25 A kind of method of Ba Shayu seedling artificial propagation Pending CN108901942A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110402854A (en) * 2019-08-09 2019-11-05 武汉中科瑞华生态科技股份有限公司 A kind of high viability breeding method of C. guichenoti fry

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