CN110402854A - A kind of high viability breeding method of C. guichenoti fry - Google Patents
A kind of high viability breeding method of C. guichenoti fry Download PDFInfo
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- CN110402854A CN110402854A CN201910734311.3A CN201910734311A CN110402854A CN 110402854 A CN110402854 A CN 110402854A CN 201910734311 A CN201910734311 A CN 201910734311A CN 110402854 A CN110402854 A CN 110402854A
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- 241000033272 Coreius guichenoti Species 0.000 title claims abstract description 39
- 238000009395 breeding Methods 0.000 title claims abstract description 24
- 230000035899 viability Effects 0.000 title claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 113
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 93
- 241000894006 Bacteria Species 0.000 claims abstract description 66
- 239000000463 material Substances 0.000 claims abstract description 59
- 239000004576 sand Substances 0.000 claims abstract description 53
- 239000002207 metabolite Substances 0.000 claims abstract description 39
- 238000000855 fermentation Methods 0.000 claims abstract description 34
- 230000004151 fermentation Effects 0.000 claims abstract description 34
- 230000004048 modification Effects 0.000 claims abstract description 24
- 238000012986 modification Methods 0.000 claims abstract description 24
- OZECDDHOAMNMQI-UHFFFAOYSA-H cerium(3+);trisulfate Chemical compound [Ce+3].[Ce+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O OZECDDHOAMNMQI-UHFFFAOYSA-H 0.000 claims abstract description 19
- 229910000333 cerium(III) sulfate Inorganic materials 0.000 claims abstract description 19
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 19
- 239000011734 sodium Substances 0.000 claims abstract description 19
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 claims abstract description 18
- 235000020681 well water Nutrition 0.000 claims abstract description 18
- 239000002349 well water Substances 0.000 claims abstract description 18
- 230000002776 aggregation Effects 0.000 claims abstract description 9
- 238000004220 aggregation Methods 0.000 claims abstract description 9
- KCZFLPPCFOHPNI-UHFFFAOYSA-N alumane;iron Chemical compound [AlH3].[Fe] KCZFLPPCFOHPNI-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000011081 inoculation Methods 0.000 claims abstract description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 50
- 229930006000 Sucrose Natural products 0.000 claims description 50
- 239000005720 sucrose Substances 0.000 claims description 50
- 230000010355 oscillation Effects 0.000 claims description 39
- 241000361919 Metaphire sieboldi Species 0.000 claims description 33
- 238000000034 method Methods 0.000 claims description 33
- 239000001963 growth medium Substances 0.000 claims description 27
- 238000004659 sterilization and disinfection Methods 0.000 claims description 26
- 239000002609 medium Substances 0.000 claims description 24
- 238000002360 preparation method Methods 0.000 claims description 21
- 238000003756 stirring Methods 0.000 claims description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 19
- 230000001580 bacterial effect Effects 0.000 claims description 19
- 241000595940 Notostraca Species 0.000 claims description 16
- 241001482311 Trionychidae Species 0.000 claims description 16
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 14
- 239000012153 distilled water Substances 0.000 claims description 14
- 230000035611 feeding Effects 0.000 claims description 14
- 238000001035 drying Methods 0.000 claims description 13
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 11
- 239000004411 aluminium Substances 0.000 claims description 10
- 229910052782 aluminium Inorganic materials 0.000 claims description 10
- 238000011218 seed culture Methods 0.000 claims description 10
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 9
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 9
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 9
- 235000019441 ethanol Nutrition 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 claims description 8
- 229920000153 Povidone-iodine Polymers 0.000 claims description 8
- 229910000831 Steel Inorganic materials 0.000 claims description 8
- 238000000498 ball milling Methods 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 8
- 239000011521 glass Substances 0.000 claims description 8
- 239000012528 membrane Substances 0.000 claims description 8
- 229960001621 povidone-iodine Drugs 0.000 claims description 8
- 108090000623 proteins and genes Proteins 0.000 claims description 8
- 102000004169 proteins and genes Human genes 0.000 claims description 8
- 239000010959 steel Substances 0.000 claims description 8
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 claims description 7
- 239000005695 Ammonium acetate Substances 0.000 claims description 7
- 229920001353 Dextrin Polymers 0.000 claims description 7
- 239000004375 Dextrin Substances 0.000 claims description 7
- 229920002472 Starch Polymers 0.000 claims description 7
- 230000004913 activation Effects 0.000 claims description 7
- 229940043376 ammonium acetate Drugs 0.000 claims description 7
- 235000019257 ammonium acetate Nutrition 0.000 claims description 7
- 239000001110 calcium chloride Substances 0.000 claims description 7
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 7
- 235000019425 dextrin Nutrition 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 7
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 7
- 239000011780 sodium chloride Substances 0.000 claims description 7
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 7
- 239000008107 starch Substances 0.000 claims description 7
- 235000019698 starch Nutrition 0.000 claims description 7
- 239000006228 supernatant Substances 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
- 229910052564 epsomite Inorganic materials 0.000 claims description 6
- 239000012299 nitrogen atmosphere Substances 0.000 claims description 6
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 238000005273 aeration Methods 0.000 claims description 2
- 150000001298 alcohols Chemical class 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 239000001888 Peptone Substances 0.000 claims 1
- 108010080698 Peptones Proteins 0.000 claims 1
- 235000019319 peptone Nutrition 0.000 claims 1
- 239000002244 precipitate Substances 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 15
- 201000010099 disease Diseases 0.000 abstract description 11
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 11
- 208000035240 Disease Resistance Diseases 0.000 abstract description 2
- 239000002054 inoculum Substances 0.000 description 12
- 230000008569 process Effects 0.000 description 12
- 238000012545 processing Methods 0.000 description 12
- 239000012467 final product Substances 0.000 description 11
- 230000000052 comparative effect Effects 0.000 description 10
- 241000251468 Actinopterygii Species 0.000 description 9
- 206010013786 Dry skin Diseases 0.000 description 9
- 238000005119 centrifugation Methods 0.000 description 9
- 230000000384 rearing effect Effects 0.000 description 9
- 239000012286 potassium permanganate Substances 0.000 description 7
- 235000018102 proteins Nutrition 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 6
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 6
- 235000011130 ammonium sulphate Nutrition 0.000 description 6
- 229960004756 ethanol Drugs 0.000 description 6
- 230000004907 flux Effects 0.000 description 6
- 238000004321 preservation Methods 0.000 description 6
- 108010009004 proteose-peptone Proteins 0.000 description 6
- 238000009423 ventilation Methods 0.000 description 6
- 229960000935 dehydrated alcohol Drugs 0.000 description 5
- 238000004090 dissolution Methods 0.000 description 5
- 241000607720 Serratia Species 0.000 description 4
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 241000607715 Serratia marcescens Species 0.000 description 3
- 239000006071 cream Substances 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 238000002386 leaching Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005189 flocculation Methods 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 210000003371 toe Anatomy 0.000 description 2
- ZELCNSAUMHNSSU-UHFFFAOYSA-N 3,5-diamino-2-[(4-sulfamoylphenyl)diazenyl]benzoic acid Chemical compound OC(=O)C1=CC(N)=CC(N)=C1N=NC1=CC=C(S(N)(=O)=O)C=C1 ZELCNSAUMHNSSU-UHFFFAOYSA-N 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 241001609213 Carassius carassius Species 0.000 description 1
- 241000272201 Columbiformes Species 0.000 description 1
- 206010011409 Cross infection Diseases 0.000 description 1
- 241000252210 Cyprinidae Species 0.000 description 1
- MQJKPEGWNLWLTK-UHFFFAOYSA-N Dapsone Chemical compound C1=CC(N)=CC=C1S(=O)(=O)C1=CC=C(N)C=C1 MQJKPEGWNLWLTK-UHFFFAOYSA-N 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 241000594009 Phoxinus phoxinus Species 0.000 description 1
- 241001282157 Polymixia lowei Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- -1 aluminium ferrum silicate ion Chemical class 0.000 description 1
- PZZYQPZGQPZBDN-UHFFFAOYSA-N aluminium silicate Chemical compound O=[Al]O[Si](=O)O[Al]=O PZZYQPZGQPZBDN-UHFFFAOYSA-N 0.000 description 1
- 229910000323 aluminium silicate Inorganic materials 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
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- 239000000084 colloidal system Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000003311 flocculating effect Effects 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- DRXYRSRECMWYAV-UHFFFAOYSA-N mercury(I) nitrate Inorganic materials [Hg+].[O-][N+]([O-])=O DRXYRSRECMWYAV-UHFFFAOYSA-N 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
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- 231100000719 pollutant Toxicity 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/281—Treatment of water, waste water, or sewage by sorption using inorganic sorbents
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/286—Treatment of water, waste water, or sewage by sorption using natural organic sorbents or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/5236—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents
- C02F1/5245—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents using basic salts, e.g. of aluminium and iron
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Environmental & Geological Engineering (AREA)
- Animal Husbandry (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
- Hydrology & Water Resources (AREA)
- Zoology (AREA)
- Marine Sciences & Fisheries (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Inorganic Chemistry (AREA)
- Health & Medical Sciences (AREA)
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- Insects & Arthropods (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides a kind of high viability breeding methods of C. guichenoti fry, and it is few to cultivate C. guichenoti cause of disease using well water as water source.For the physiological requirements of C. guichenoti fry different phase, different feeds is provided in the different stages, using agreeable to the taste corresponding bait, effectively raises fry growth speed, fry is healthy and strong, and constitution is good, and disease resistance is good, and fry survival rate is high.Water-bed material be using modification infusorial earth load aggregation aluminium iron silicate, cerous sulfate, sodium humate, grignard sand thunder bacterium metabolite (grignard sand thunder bacterium ACCC01695 is activated, inoculation, fermentation obtain) and obtain.Improve water body environment, substantially reduce disease incidence, improves survival rate.
Description
Technical field
The present invention relates to technical field of aquaculture, more particularly to a kind of high viability cultivation side of C. guichenoti fry
Method.
Background technique
C. guichenoti Coreius guichenoti (Sauvage et Dabry) is under the jurisdiction of Cyprinidae, Minnow subfamily, copper fish category,
Local name has: square toes, watertight, round mouth, fertile a small bay in a river, square toes watertight, husky shell crucian carp (Ming River), peace fish for cats (Yunnan piece beauty), pigeon
Fish, beard fish (Jinsha jiang River middle reaches), fried dough twist fish etc..It is common under Upper Yangtze River mainstream, Jia Lingjiang River middle and lower reaches, Tuo Jiang, Jinsha jiang River
The water systems such as trip, Lower Reaches of Wujiang River (Ding Ruihua, 1995；Liu Lehe etc., 1990), it is Upper Yangtze River Endemic fish and important economic fish
Class.C. guichenoti meat tenderness is fertile, is rich in fatty (Ding Ruihua, 1995), is the important economic fish of Upper Yangtze River and rivers fishery
Important fished species.
However since overfishing, power plant construction are to caused by the destruction in C. guichenoti spawning ground and the fast development of industry
The influence of the factors such as water pollution, so that the wild resource of C. guichenoti sharply declines (Yang Zhi, 2009), therefore C. guichenoti object
Kind protection work is urgently carried out.Artificial propagation of fish and enhancement releasing are current development species conservations, increase having for population quantity
One of effect measure.The acquisition of perfect wild parent population and raise and train, artificial breeding and extensive seed rearing are to guarantee Technique in Fishes
The enhancement releasing prerequisite that everything goes well with your work carries out.
At present about C. guichenoti research report relate generally to biology (remaining will hall etc., 1984；Zheng Shuming, 1988；It is yellow
Lin in Xiu and Deng, 1990；Zheng Shuming and Wu Qing, 1998；Cheong Kuoc Va etc., 1999；Ge Qingxiu etc., 2001；Zhang Xianfang etc., 2005),
Physiology and biochemistry (Wang Youhui etc., 2005；Wang Youhui etc., 2005；Li Rong etc., 2008；Liu Jun etc., 2008；, point Wu Bin etc., 2008)
Sub- science of heredity (Liao little Lin, 2006；Xu Shuying, 2007；Yuan Xi equality, 2008；Kong Yan, 2010), stock number (Yu Gongliang etc.,
2002；Yang Zhi, 2009；Yang Zhi etc., 2011), ecological habit (Liu Lehe etc., 1990；Cao Wenxuan, 2008) etc., C. guichenoti fish
The artificial culture of seedling is also in a primary research stage, and conventional fry rearing method disease incidence is high, survival rate bottom.
Summary of the invention
Present invention aim to provide a kind of high viability breeding method of C. guichenoti fry, breeding method letter
It is single, it is easy to operate, fry disease incidence is reduced, fry survival rate is improved.
To achieve the above object, the present invention is achieved by the following scheme:
A kind of high viability breeding method of C. guichenoti fry, comprising the following steps:
(1) selection of Cultivation container；
(2) water quality management: being laid with one layer of water-bed material at the bottom, and pollution-free well water is educated as cultivation water source miniflow water planting,
It is aerated using oxygen increasing pump whole day；
(3) fry is put in a suitable place to breed, and different phase uses different bait feedings；
Wherein, the water-bed material is to utilize modification infusorial earth load aggregation aluminium iron silicate, cerous sulfate, sodium humate, lattice
Family name's sand thunder bacterium metabolite and obtain, the grignard sand thunder bacterium metabolite be grignard sand thunder bacterium ACCC01695 is activated, connect
Kind, fermentation obtain.
Preferably, water-bed material with a thickness of 5~8cm.
Preferably, the grignard sand thunder bacterium metabolite the preparation method is as follows: grignard sand thunder bacterium ACCC01695 activation after
It is inoculated in seed culture medium, 28~31 DEG C are cultivated 12~15 hours, and bacterial strain seed liquor is obtained；Bacterial strain seed liquor is taken to be inoculated in fermentation
In culture medium, fermented supernatant fluid is collected in 27~31 DEG C, 180~200rpm/min shaken cultivation, centrifugation, is precipitated to get a kind of lattice
Family name's sand thunder bacterium metabolite；Wherein, the formula of seed culture medium are as follows: 8~12g of casein peptone, 6~9g of soluble starch, dextrin 5
~8g, 3~5g of sodium chloride, ammonium acetate 2~4g, FeSO40.2~0.3g, CaCl23~4g, distilled water 900~1100mL, pH=
6.8~7.2,121 DEG C sterilize 20~30 minutes；The formula of fermentation medium are as follows: 4~6g of yeast extract,13C flag sucrose 0.2~
0.3g, MgSO4·7H2O5~8g, NaNO38~12g of 5~8g, KCl, 900~1100mL of distilled water, pH=6.8~7.2,
121 DEG C sterilize 20~30 minutes；13C flag sucrose terminates to cultivate when exhausting.
It is further preferred that being activated using LB culture medium, specific method is: by grignard sand thunder bacterium
ACCC01695 is transferred on LB culture medium from taking-up in the test tube slant of preservation of bacteria strain, and inoculum concentration is percentage by volume 2~3%,
Culture 24~72 hours is inverted in 28 DEG C of insulating boxs.
It is further preferred that fermented and cultured is ventilation culture, air flux is 10~12L/min.
It is further preferred that the inoculum concentration of bacterial strain seed liquor in the fermentation medium is percentage by volume 6~8%.
It is further preferred that 1/2 formula ratio is first added when preparing fermentation medium13C flag sucrose, shaken cultivation process
Middle monitoring13The dosage of C flag sucrose,13C flag sucrose adds surplus when exhausting in batches13C flag sucrose, until all
's13C flag sucrose all exhausts, and culture terminates；13The number of adding of C flag sucrose is 3~5 times, and each additional amount is identical.
It is further preferred that centrifugal condition are as follows: 8000~12000rpm/min is centrifuged 15~20 minutes；Intermediate processing are as follows:
It is precipitated and is collected using 50~80% ammonium sulfate of mass concentration.
Preferably, the modification infusorial earth the preparation method is as follows: in parts by weight, by 1 part of neopelex
It is added in the water of 5~8 times of weight, stirring and dissolving, is then heated with stirring to 70~80 DEG C, 2~3 parts of activated diatomaceous earths, nitrogen is added
It stirs 3~4 hours, filters under gas atmosphere, washing is drying to obtain.
It is further preferred that the activated diatomaceous earth is diatomite to be added in the water of 5~6 times of weight, 800~1000W
Microwave treatment 3~5 minutes, centrifugation, 120~130 DEG C of dryings 2~3 hours to obtain the final product.
It is further preferred that dry process conditions are as follows: 60~70 DEG C drying 12~18 hours.
Preferably, the water-bed material the preparation method is as follows: in parts by weight, by 1 part of polymeric aluminium ferrum silicate, 0.1~
0.2 part of cerous sulfate, 3~4 parts of sodium humates, 0.3~0.4 part of grignard sand thunder bacterium metabolite pour into 15~20 parts of dehydrated alcohols
In, then 8~10 parts of modification infusorial earths, second of supersonic oscillations, microwave is added in first time supersonic oscillations, abundant ball milling
Processing, third time supersonic oscillations, ethyl alcohol are volatilized naturally to get the water-bed material.
It is further preferred that the place of first time supersonic oscillations, second of supersonic oscillations and third time supersonic oscillations
Managing the time is respectively 30~40 minutes, 10~15 minutes, 30~40 minutes.
It is further preferred that the process conditions of microwave treatment are as follows: 600~800W microwave treatment 3~5 minutes.
Preferably, select the circular glass steel culturing jar of diameter 2m, 0.8~1m of depth as Cultivation container in step (1).
Preferably, in step (2), pollution-free well water is injected after Cultivation container is disinfected in advance, the depth of water 0.6~
0.8m is disinfected for further preferably in advance 1~2 day using 10ppm potassium permanganate.
Preferably, the specific method of step (3) is: first setting up net cage in Cultivation container, selects 4~5 days round mouths of membrane
Copper fish and water flower seedling is put into raising in cage, 2000~3000 tails of density/m3, it 18~22 DEG C of fry rearing water temperature, feeds daily 4 times,
2~3g/ of fairy shrimp, ten thousand tail is fed within 1st~10 day, 4~5g/ of fairy shrimp, ten thousand tail is fed within 11~20 days；It is after 10 days that fry point is dilute simultaneously
It is transferred to Cultivation container from net cage directly to cultivate, 1000~1500 tails of density/m3, reach 2cm or so within 20 days, using the water earthworm minced
It is fed after earthworm disinfection, by dilute, 500~800 tails of density/m of fry point after 30 days3, reach 4cm or so within 60 days, hereafter use soft-shelled turtle
Production dough is fed after material, water earthworm are mixed according to mass ratio 1:2~4.
It is further preferred that the net cage aperture is less than 1.0mm, the size of net cage is 1.4m*1.4m*0.6m.
It is further preferred that feeding daily 4 times, time 8:00,12:00,18:00,24:00.
It is further preferred that the water earthworm size minced is less than 1mm, water earthworm disinfection is soaked using 5ppm povidone iodine
Bubble 30 minutes；The protein quality content of soft-shelled turtle material is greater than 45%.
The beneficial effects of the present invention are:
The present invention is laid with one layer of water-bed material at the bottom, and pollution-free well water is educated as cultivation water source miniflow water planting, using increasing
Oxygen pump whole day aeration, fry are put in a suitable place to breed, and different phase uses different bait feedings, greatly reduce fry by above-mentioned breeding method
Disease incidence improves fry survival rate.It is specific as follows:
1, it is few to be cultivated as water source using well water for C. guichenoti cause of disease.C. guichenoti is cultivated with river and lake water source easily to be infected
Ich, the treatment method without significant effective in addition to mercurous nitrate (being forbidden to use), routine cultivate the death rate to the disease at present
It is high.
2, for the physiological requirements of C. guichenoti fry different phase, different feeds is provided in the different stages, is used
Agreeable to the taste corresponding bait effectively raises fry growth speed, and fry is healthy and strong, and constitution is good, and disease resistance is good, fry survival
Rate is high.
3, water-bed material is to utilize modification infusorial earth load aggregation aluminium iron silicate, cerous sulfate, sodium humate, grignard sand thunder bacterium
Metabolite (grignard sand thunder bacterium ACCC01695 is activated, inoculation, fermentation obtain) and obtain.Polymeric aluminium ferrum silicate ion degree is high, tool
There is the double action of flocculation and disinfection；Cerous sulfate after ball-milling treatment there is powerful electrical neutralization and net to catch volume and sweep work
With strengthening flocculating effect；Sodium humate has very strong adsorption capacity, and has colloid property, can promote feed ingredient
Activated absorption；Grignard sand thunder bacterium metabolite has bactericidal effect, and the immunity of C. guichenoti can be improved, substantially reduce morbidity
Rate improves survival rate.The purification of polymeric aluminium ferrum silicate, cerous sulfate, sodium humate cooperative achievement to water body improves water body environment,
Disease incidence is substantially reduced, survival rate is improved.The present invention is loaded using modification infusorial earth, with porous structure, large specific surface area,
On the one hand play the role of adsorption-flocculation, purifying water body, the polymer aluminium silicate on the other hand loaded to it to the pollutant in water body
Iron, cerous sulfate, sodium humate, grignard sand thunder bacterium metabolite etc. play similar slow releasing function, long-term slow release, thus for a long time
Above-mentioned effect is played, plays the role of reducing disease incidence.
4, using small dimension Cultivation container, it is effectively reduced the cross-infection of disease.
5, the laying depth of water-bed material should be adapted with the depth of water, and thickness accounting is too small, and to can not achieve good water body net
Change, causes disease incidence to increase, influence fry survival rate；Thickness accounting crosses the living space that conference ties up C. guichenoti fry,
It will lead to fry survival rate to be lower.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
Grignard sand thunder bacterium ACCC01695 of the present invention is purchased from Chinese agriculture Microbiological Culture Collection administrative center.
Embodiment 1
A kind of high viability breeding method of C. guichenoti fry, comprising the following steps:
(1) selection of Cultivation container；
(2) water quality management: being laid with one layer of water-bed material at the bottom, and pollution-free well water is educated as cultivation water source miniflow water planting,
It is aerated using oxygen increasing pump whole day；
(3) fry is put in a suitable place to breed, and different phase uses different bait feedings；
Wherein, water-bed material is husky using modification infusorial earth load aggregation aluminium iron silicate, cerous sulfate, sodium humate, grignard
Thunder bacterium metabolite and obtain, the grignard sand thunder bacterium metabolite be grignard sand thunder bacterium ACCC01695 is activated, inoculation,
Fermentation obtains.Water-bed material with a thickness of 5cm.
Grignard sand thunder bacterium metabolite the preparation method is as follows: grignard sand thunder bacterium ACCC01695 activation after be inoculated in seed
In culture medium, 28 DEG C are cultivated 12 hours, obtain bacterial strain seed liquor；Bacterial strain seed liquor is taken to be inoculated in fermentation medium, 27 DEG C,
Fermented supernatant fluid is collected in 180rpm/min shaken cultivation, centrifugation, is precipitated to get a kind of grignard sand thunder bacterium metabolite；Wherein,
The formula of seed culture medium are as follows: casein peptone 8g, soluble starch 6g, dextrin 5g, sodium chloride 3g, ammonium acetate 2g, FeSO4
0.2g, CaCl23g, 900mL, pH=6.8,121 DEG C of distilled water sterilize 20 minutes；The formula of fermentation medium are as follows: yeast extract
4g,13C flag sucrose 0.2g, MgSO4·7H2O 5g, NaNO35g, KCl 8g, 900mL, pH=6.8,121 DEG C of distilled water sterilizings
20 minutes；13C flag sucrose terminates to cultivate when exhausting.It is activated using LB culture medium, specific method is: by grignard sand
Thunder bacterium ACCC01695 is transferred on LB culture medium from taking-up in the test tube slant of preservation of bacteria strain, and inoculum concentration is percentage by volume
2%, culture 24 hours is inverted in 28 DEG C of insulating boxs.Fermented and cultured is ventilation culture, air flux 10L/min.Bacterium
The inoculum concentration of strain seed liquor in the fermentation medium is percentage by volume 6%.1/2 formula ratio is first added when preparing fermentation medium
's13C flag sucrose, shaken cultivation monitor in the process13The dosage of C flag sucrose,13C flag sucrose adds residue when exhausting in batches
Amount13C flag sucrose, until all13C flag sucrose all exhausts, and culture terminates；13The number of adding of C flag sucrose is 3
Secondary, each additional amount is identical.Centrifugal condition are as follows: 8000rpm/min is centrifuged 15 minutes；Intermediate processing are as follows: utilize mass concentration
50% ammonium sulfate is precipitated and is collected.
Modification infusorial earth the preparation method is as follows: by 1kg neopelex be added 5 times of weight water in, stirring
Then dissolution is heated with stirring to 70 DEG C, 2kg activated diatomaceous earth is added, stirs 3 hours under nitrogen atmosphere, filters, washing, and 60 DEG C
Dry 12 hours to obtain the final product.Activated diatomaceous earth is diatomite to be added in the water of 5 times of weight, 800W microwave treatment 3 minutes, centrifugation,
120 DEG C of dryings 2 hours to obtain the final product.
Water-bed material the preparation method is as follows: by 1kg polymeric aluminium ferrum silicate, 0.1kg cerous sulfate, 3kg sodium humate,
0.3kg grignard sand thunder bacterium metabolite pours into 15kg dehydrated alcohol, then first time supersonic oscillations, abundant ball milling is added
8kg modification infusorial earth, second of supersonic oscillations, microwave treatment, third time supersonic oscillations, ethyl alcohol are volatilized naturally to get institute
State water-bed material.The processing time of first time supersonic oscillations, second of supersonic oscillations and third time supersonic oscillations is distinguished
It is 30 minutes, 10 minutes, 30 minutes.The process conditions of microwave treatment are as follows: 600W microwave treatment 3 minutes.
Select the circular glass steel culturing jar of diameter 2m, depth 0.8m as Cultivation container in step (1).
In step (2), by Cultivation container shift to an earlier date 1 day disinfection treatment (disinfection treatment of 10ppm potassium permanganate) inject no dirt afterwards
Contaminate well water, depth of water 0.6m.
The specific method of step (3) is: first setting up net cage in Cultivation container, selects 4 days C. guichenoti spray seedlings of membrane
It is put into raising in cage, 3000 tails of density/m3, 18 DEG C of fry rearing water temperature, feed daily 4 times, feed fairy shrimp within the 1st~10 day
Ten thousand tail of 2g/ feeds ten thousand tail of fairy shrimp 4g/ for 11~20 days；It is fry is point dilute and be transferred to Cultivation container from net cage and directly train after 10 days
It educates, 1000 tails of density/m3, reach 2cm or so within 20 days, is fed after being sterilized using the water earthworm minced, it is after 30 days that fry point is dilute,
500 tails of density/m3, reach 4cm or so within 60 days, make dough after hereafter mixing using soft-shelled turtle material, water earthworm according to mass ratio 1:2
It feeds.Net cage aperture is less than 1.0mm, and the size of net cage is 1.4m*1.4m*0.6m.It feeds 4 times daily, time 8:00,
12:00,18:00,24:00.The water earthworm size minced is less than 1mm, and water earthworm disinfection impregnates 30 points using 5ppm povidone iodine
Clock；The protein quality content of soft-shelled turtle material is greater than 45%.
Embodiment 2
A kind of high viability breeding method of C. guichenoti fry, comprising the following steps:
(1) selection of Cultivation container；
(2) water quality management: being laid with one layer of water-bed material at the bottom, and pollution-free well water is educated as cultivation water source miniflow water planting,
It is aerated using oxygen increasing pump whole day；
(3) fry is put in a suitable place to breed, and different phase uses different bait feedings；
Wherein, water-bed material is husky using modification infusorial earth load aggregation aluminium iron silicate, cerous sulfate, sodium humate, grignard
Thunder bacterium metabolite and obtain, the grignard sand thunder bacterium metabolite be grignard sand thunder bacterium ACCC01695 is activated, inoculation,
Fermentation obtains.Water-bed material with a thickness of 8cm.
Grignard sand thunder bacterium metabolite the preparation method is as follows: grignard sand thunder bacterium ACCC01695 activation after be inoculated in seed
In culture medium, 31 DEG C are cultivated 15 hours, obtain bacterial strain seed liquor；Bacterial strain seed liquor is taken to be inoculated in fermentation medium, 31 DEG C,
Fermented supernatant fluid is collected in 200rpm/min shaken cultivation, centrifugation, is precipitated to get a kind of grignard sand thunder bacterium metabolite；Wherein,
The formula of seed culture medium are as follows: casein peptone 12g, soluble starch 9g, dextrin 8g, sodium chloride 5g, ammonium acetate 4g, FeSO4
0.3g, CaCl24g, 1100mL, pH=7.2,121 DEG C of distilled water sterilize 30 minutes；The formula of fermentation medium are as follows: yeast leaching
Cream 6g,13C flag sucrose 0.3g, MgSO4·7H2O 8g, NaNO38g, KCl12g, distilled water 1100mL, pH=7.2,121 DEG C
Sterilizing 30 minutes；13C flag sucrose terminates to cultivate when exhausting.It is activated using LB culture medium, specific method is: by lattice
Family name's sand thunder bacterium ACCC01695 is transferred on LB culture medium from taking-up in the test tube slant of preservation of bacteria strain, and inoculum concentration is volume basis
Number 3% is inverted culture 72 hours in 28 DEG C of insulating boxs.Fermented and cultured is ventilation culture, air flux 12L/min.
The inoculum concentration of bacterial strain seed liquor in the fermentation medium is percentage by volume 8%.1/2 formula is first added when preparing fermentation medium
Amount13C flag sucrose, shaken cultivation monitor in the process13The dosage of C flag sucrose,13It is added in batches when C flag sucrose exhausts surplus
Surplus13C flag sucrose, until all13C flag sucrose all exhausts, and culture terminates；13C flag sucrose adds number
It is 5 times, each additional amount is identical.Centrifugal condition are as follows: 12000rpm/min is centrifuged 20 minutes；Intermediate processing are as follows: dense using quality
80% ammonium sulfate is spent to be precipitated and collected.
Modification infusorial earth the preparation method is as follows: by 1kg neopelex be added 8 times of weight water in, stirring
Then dissolution is heated with stirring to 80 DEG C, 3kg activated diatomaceous earth is added, stirs 4 hours under nitrogen atmosphere, filters, washing, and 70 DEG C
Dry 18 hours to obtain the final product.Activated diatomaceous earth is diatomite to be added in the water of 6 times of weight, 1000W microwave treatment 5 minutes, centrifugation,
130 DEG C of dryings 3 hours to obtain the final product.
Water-bed material the preparation method is as follows: by 1kg polymeric aluminium ferrum silicate, 0.2kg cerous sulfate, 4kg sodium humate,
0.4kg grignard sand thunder bacterium metabolite pours into 20kg dehydrated alcohol, then first time supersonic oscillations, abundant ball milling is added
10kg modification infusorial earth, second of supersonic oscillations, microwave treatment, third time supersonic oscillations, ethyl alcohol are volatilized naturally to get institute
State water-bed material.The processing time of first time supersonic oscillations, second of supersonic oscillations and third time supersonic oscillations is distinguished
It is 40 minutes, 15 minutes, 40 minutes.The process conditions of microwave treatment are as follows: 800W microwave treatment 5 minutes.
Select the circular glass steel culturing jar of diameter 2m, depth 1m as Cultivation container in step (1).
In step (2), by Cultivation container shift to an earlier date 2 days disinfection treatment (disinfection treatment of 10ppm potassium permanganate) inject no dirt afterwards
Contaminate well water, depth of water 0.8m.
The specific method of step (3) is: first setting up net cage in Cultivation container, selects 5 days C. guichenoti spray seedlings of membrane
It is put into raising in cage, 2000 tails of density/m3, 22 DEG C of fry rearing water temperature, feed daily 4 times, feed fairy shrimp within the 1st~10 day
Ten thousand tail of 3g/ feeds ten thousand tail of fairy shrimp 5g/ for 11~20 days；It is fry is point dilute and be transferred to Cultivation container from net cage and directly train after 10 days
It educates, 1500 tails of density/m3, reach 2cm or so within 20 days, is fed after being sterilized using the water earthworm minced, it is after 30 days that fry point is dilute,
800 tails of density/m3, reach 4cm or so within 60 days, make dough after hereafter mixing using soft-shelled turtle material, water earthworm according to mass ratio 1:4
It feeds.Net cage aperture is less than 1.0mm, and the size of net cage is 1.4m*1.4m*0.6m.It feeds 4 times daily, time 8:00,
12:00,18:00,24:00.The water earthworm size minced is less than 1mm, and water earthworm disinfection impregnates 30 points using 5ppm povidone iodine
Clock；The protein quality content of soft-shelled turtle material is greater than 45%.
Embodiment 3
A kind of high viability breeding method of C. guichenoti fry, comprising the following steps:
(1) selection of Cultivation container；
(2) water quality management: being laid with one layer of water-bed material at the bottom, and pollution-free well water is educated as cultivation water source miniflow water planting,
It is aerated using oxygen increasing pump whole day；
(3) fry is put in a suitable place to breed, and different phase uses different bait feedings；
Wherein, water-bed material is husky using modification infusorial earth load aggregation aluminium iron silicate, cerous sulfate, sodium humate, grignard
Thunder bacterium metabolite and obtain, the grignard sand thunder bacterium metabolite be grignard sand thunder bacterium ACCC01695 is activated, inoculation,
Fermentation obtains.Water-bed material with a thickness of 6cm.
Grignard sand thunder bacterium metabolite the preparation method is as follows: grignard sand thunder bacterium ACCC01695 activation after be inoculated in seed
In culture medium, 30 DEG C are cultivated 14 hours, obtain bacterial strain seed liquor；Bacterial strain seed liquor is taken to be inoculated in fermentation medium, 29 DEG C,
Fermented supernatant fluid is collected in 190rpm/min shaken cultivation, centrifugation, is precipitated to get a kind of grignard sand thunder bacterium metabolite；Wherein,
The formula of seed culture medium are as follows: casein peptone 10g, soluble starch 8g, dextrin 6g, sodium chloride 4g, ammonium acetate 3g, FeSO4
0.25g, CaCl23.5g, 1000mL, pH=7,121 DEG C of distilled water sterilize 25 minutes；The formula of fermentation medium are as follows: yeast leaching
Cream 5g,13C flag sucrose 0.25g, MgSO4·7H2O 6g, NaNO37g, KCl 10g, distilled water 1000mL, pH=7.1,121
DEG C sterilizing 24 minutes；13C flag sucrose terminates to cultivate when exhausting.It is activated using LB culture medium, specific method is: will
Grignard sand thunder bacterium ACCC01695 is transferred on LB culture medium from taking-up in the test tube slant of preservation of bacteria strain, and inoculum concentration is volume hundred
Score 2.5% is inverted culture 36 hours in 28 DEG C of insulating boxs.Fermented and cultured is ventilation culture, air flux 11L/
min.The inoculum concentration of bacterial strain seed liquor in the fermentation medium is percentage by volume 7%.1/2 is first added when preparing fermentation medium
Formula ratio13C flag sucrose, shaken cultivation monitor in the process13The dosage of C flag sucrose,13C flag sucrose is mended in batches when exhausting
Add surplus13C flag sucrose, until all13C flag sucrose all exhausts, and culture terminates；13C flag sucrose is added
Number is 4 times, and each additional amount is identical.Centrifugal condition are as follows: 11000rpm/min is centrifuged 18 minutes；Intermediate processing are as follows: utilize matter
Amount 70% ammonium sulfate of concentration is precipitated and is collected.
Modification infusorial earth the preparation method is as follows: by 1kg neopelex be added 6 times of weight water in, stirring
Then dissolution is heated with stirring to 75 DEG C, 2.5kg activated diatomaceous earth is added, stirs 3.5 hours under nitrogen atmosphere, filters, washing,
65 DEG C of dryings 15 hours to obtain the final product.Activated diatomaceous earth is diatomite to be added in the water of 5 times of weight, 900W microwave treatment 4 minutes, from
The heart, 125 DEG C of dryings 2 hours to obtain the final product.
Water-bed material the preparation method is as follows: by 1kg polymeric aluminium ferrum silicate, 0.15kg cerous sulfate, 3.5kg sodium humate,
0.35kg grignard sand thunder bacterium metabolite pours into 18kg dehydrated alcohol, then first time supersonic oscillations, abundant ball milling is added
9kg modification infusorial earth, second of supersonic oscillations, microwave treatment, third time supersonic oscillations, ethyl alcohol are volatilized naturally to get institute
State water-bed material.The processing time of first time supersonic oscillations, second of supersonic oscillations and third time supersonic oscillations is distinguished
It is 35 minutes, 12 minutes, 33 minutes.The process conditions of microwave treatment are as follows: 750W microwave treatment 4 minutes.
Select the circular glass steel culturing jar of diameter 2m, depth 0.9m as Cultivation container in step (1).
In step (2), by Cultivation container shift to an earlier date 2 days disinfection treatment (disinfection treatment of 10ppm potassium permanganate) inject no dirt afterwards
Contaminate well water, depth of water 0.7m.
The specific method of step (3) is: first setting up net cage in Cultivation container, selects 4 days C. guichenoti spray seedlings of membrane
It is put into raising in cage, 2500 tails of density/m3, 20 DEG C of fry rearing water temperature, feed daily 4 times, feed fairy shrimp within the 1st~10 day
Ten thousand tail of 2.5g/ feeds ten thousand tail of fairy shrimp 4.5g/ for 11~20 days；It is fry is point dilute and to be transferred to Cultivation container from net cage straight after 10 days
Connect cultivation, 1200 tails of density/m3, reach 2cm or so within 20 days, using feeding after the water earthworm disinfection minced, by fry after 30 days
Divide dilute, 700 tails of density/m3, reach 4cm or so within 60 days, made after hereafter being mixed using soft-shelled turtle material, water earthworm according to mass ratio 1:3
It is fed as dough.Net cage aperture is less than 1.0mm, and the size of net cage is 1.4m*1.4m*0.6m.It feeds 4 times daily, the time is
8:00,12:00,18:00,24:00.The water earthworm size minced is less than 1mm, and water earthworm disinfection is impregnated using 5ppm povidone iodine
30 minutes；The protein quality content of soft-shelled turtle material is greater than 45%.
Comparative example 1
A kind of high viability breeding method of C. guichenoti fry, comprising the following steps:
(1) selection of Cultivation container；
(2) water quality management: being laid with one layer of water-bed material at the bottom, and pollution-free well water is educated as cultivation water source miniflow water planting,
It is aerated using oxygen increasing pump whole day；
(3) fry is put in a suitable place to breed, and different phase uses different bait feedings；
Wherein, water-bed material is husky using modification infusorial earth load aggregation aluminium iron silicate, cerous sulfate, sodium humate, cement
Thunder Salmonella metabolite and obtain, the serratia marcescens metabolite be Serratia marcescans ATCC 14756 is activated,
Inoculation, fermentation obtain.Water-bed material with a thickness of 6cm.
Serratia marcescens metabolite the preparation method is as follows: Serratia marcescans ATCC 14756 activation after be inoculated in
In seed culture medium, 30 DEG C are cultivated 14 hours, obtain bacterial strain seed liquor；Bacterial strain seed liquor is taken to be inoculated in fermentation medium, 29 DEG C,
Fermented supernatant fluid is collected in 190rpm/min shaken cultivation, centrifugation, is precipitated to get a kind of serratia marcescens metabolite；Its
In, the formula of seed culture medium are as follows: casein peptone 10g, soluble starch 8g, dextrin 6g, sodium chloride 4g, ammonium acetate 3g,
FeSO40.25g, CaCl23.5g, 1000mL, pH=7,121 DEG C of distilled water sterilize 25 minutes；The formula of fermentation medium are as follows: ferment
Female medicinal extract 5g,13C flag sucrose 0.25g, MgSO4·7H2O 6g, NaNO37g, KCl 10g, distilled water 1000mL, pH=7.1,
121 DEG C sterilize 24 minutes；13C flag sucrose terminates to cultivate when exhausting.It is activated using LB culture medium, specific method is:
Serratia marcescans ATCC 14756 is transferred on LB culture medium from taking-up in the test tube slant of preservation of bacteria strain, inoculum concentration is body
Percentage 2.5% is accumulated, is inverted culture 36 hours in 28 DEG C of insulating boxs.Fermented and cultured is ventilation culture, and air flux is
11L/min.The inoculum concentration of bacterial strain seed liquor in the fermentation medium is percentage by volume 7%.Prepare fermentation medium Shi Xianjia
Enter 1/2 formula ratio13C flag sucrose, shaken cultivation monitor in the process13The dosage of C flag sucrose,13When C flag sucrose exhausts
Surplus is added in batches13C flag sucrose, until all13C flag sucrose all exhausts, and culture terminates；13C flag sucrose
Add number be 4 times, each additional amount is identical.Centrifugal condition are as follows: 11000rpm/min is centrifuged 18 minutes；Intermediate processing are as follows:
It is precipitated and is collected using 70% ammonium sulfate of mass concentration.
Modification infusorial earth the preparation method is as follows: by 1kg neopelex be added 6 times of weight water in, stirring
Then dissolution is heated with stirring to 75 DEG C, 2.5kg activated diatomaceous earth is added, stirs 3.5 hours under nitrogen atmosphere, filters, washing,
65 DEG C of dryings 15 hours to obtain the final product.Activated diatomaceous earth is diatomite to be added in the water of 5 times of weight, 900W microwave treatment 4 minutes, from
The heart, 125 DEG C of dryings 2 hours to obtain the final product.
Water-bed material the preparation method is as follows: by 1kg polymeric aluminium ferrum silicate, 0.15kg cerous sulfate, 3.5kg sodium humate,
0.35kg grignard sand thunder bacterium metabolite pours into 18kg dehydrated alcohol, then first time supersonic oscillations, abundant ball milling is added
9kg modification infusorial earth, second of supersonic oscillations, microwave treatment, third time supersonic oscillations, ethyl alcohol are volatilized naturally to get institute
State water-bed material.The processing time of first time supersonic oscillations, second of supersonic oscillations and third time supersonic oscillations is distinguished
It is 35 minutes, 12 minutes, 33 minutes.The process conditions of microwave treatment are as follows: 750W microwave treatment 4 minutes.
Select the circular glass steel culturing jar of diameter 2m, depth 0.9m as Cultivation container in step (1).
In step (2), by Cultivation container shift to an earlier date 2 days disinfection treatment (disinfection treatment of 10ppm potassium permanganate) inject no dirt afterwards
Contaminate well water, depth of water 0.7m.
The specific method of step (3) is: first setting up net cage in Cultivation container, selects 4 days C. guichenoti spray seedlings of membrane
It is put into raising in cage, 2500 tails of density/m3, 20 DEG C of fry rearing water temperature, feed daily 4 times, feed fairy shrimp within the 1st~10 day
Ten thousand tail of 2.5g/ feeds ten thousand tail of fairy shrimp 4.5g/ for 11~20 days；It is fry is point dilute and to be transferred to Cultivation container from net cage straight after 10 days
Connect cultivation, 1200 tails of density/m3, reach 2cm or so within 20 days, using feeding after the water earthworm disinfection minced, by fry after 30 days
Divide dilute, 700 tails of density/m3, reach 4cm or so within 60 days, made after hereafter being mixed using soft-shelled turtle material, water earthworm according to mass ratio 1:3
It is fed as dough.Net cage aperture is less than 1.0mm, and the size of net cage is 1.4m*1.4m*0.6m.It feeds 4 times daily, the time is
8:00,12:00,18:00,24:00.The water earthworm size minced is less than 1mm, and water earthworm disinfection is impregnated using 5ppm povidone iodine
30 minutes；The protein quality content of soft-shelled turtle material is greater than 45%.
Comparative example 2
A kind of high viability breeding method of C. guichenoti fry, comprising the following steps:
(1) selection of Cultivation container；
(2) water quality management: being laid with one layer of water-bed material at the bottom, and pollution-free well water is educated as cultivation water source miniflow water planting,
It is aerated using oxygen increasing pump whole day；
(3) fry is put in a suitable place to breed, and different phase uses different bait feedings；
Wherein, water-bed material is metabolized using modification infusorial earth load aggregation aluminium iron silicate, sodium humate, grignard sand thunder bacterium
Product and obtain, the grignard sand thunder bacterium metabolite be grignard sand thunder bacterium ACCC01695 is activated, inoculation, fermentation obtain.
Water-bed material with a thickness of 6cm.
Grignard sand thunder bacterium metabolite the preparation method is as follows: grignard sand thunder bacterium ACCC01695 activation after be inoculated in seed
In culture medium, 30 DEG C are cultivated 14 hours, obtain bacterial strain seed liquor；Bacterial strain seed liquor is taken to be inoculated in fermentation medium, 29 DEG C,
Fermented supernatant fluid is collected in 190rpm/min shaken cultivation, centrifugation, is precipitated to get a kind of grignard sand thunder bacterium metabolite；Wherein,
The formula of seed culture medium are as follows: casein peptone 10g, soluble starch 8g, dextrin 6g, sodium chloride 4g, ammonium acetate 3g, FeSO4
0.25g, CaCl23.5g, 1000mL, pH=7,121 DEG C of distilled water sterilize 25 minutes；The formula of fermentation medium are as follows: yeast leaching
Cream 5g,13C flag sucrose 0.25g, MgSO4·7H2O 6g, NaNO37g, KCl 10g, distilled water 1000mL, pH=7.1,121
DEG C sterilizing 24 minutes；13C flag sucrose terminates to cultivate when exhausting.It is activated using LB culture medium, specific method is: will
Grignard sand thunder bacterium ACCC01695 is transferred on LB culture medium from taking-up in the test tube slant of preservation of bacteria strain, and inoculum concentration is volume hundred
Score 2.5% is inverted culture 36 hours in 28 DEG C of insulating boxs.Fermented and cultured is ventilation culture, air flux 11L/
min.The inoculum concentration of bacterial strain seed liquor in the fermentation medium is percentage by volume 7%.1/2 is first added when preparing fermentation medium
Formula ratio13C flag sucrose, shaken cultivation monitor in the process13The dosage of C flag sucrose,13C flag sucrose is mended in batches when exhausting
Add surplus13C flag sucrose, until all13C flag sucrose all exhausts, and culture terminates；13C flag sucrose is added
Number is 4 times, and each additional amount is identical.Centrifugal condition are as follows: 11000rpm/min is centrifuged 18 minutes；Intermediate processing are as follows: utilize matter
Amount 70% ammonium sulfate of concentration is precipitated and is collected.
Modification infusorial earth the preparation method is as follows: by 1kg neopelex be added 6 times of weight water in, stirring
Then dissolution is heated with stirring to 75 DEG C, 2.5kg activated diatomaceous earth is added, stirs 3.5 hours under nitrogen atmosphere, filters, washing,
65 DEG C of dryings 15 hours to obtain the final product.Activated diatomaceous earth is diatomite to be added in the water of 5 times of weight, 900W microwave treatment 4 minutes, from
The heart, 125 DEG C of dryings 2 hours to obtain the final product.
Water-bed material the preparation method is as follows: by 1kg polymeric aluminium ferrum silicate, 3.5kg sodium humate, 0.35kg grignard sand thunder
Bacterium metabolite pours into 18kg dehydrated alcohol, first time supersonic oscillations, abundant ball milling, and 9kg modification infusorial earth is then added,
Second of supersonic oscillations, microwave treatment, third time supersonic oscillations, ethyl alcohol are volatilized naturally to get the water-bed material.First
The processing time of secondary supersonic oscillations, second of supersonic oscillations and third time supersonic oscillations is respectively 35 minutes, 12 minutes,
33 minutes.The process conditions of microwave treatment are as follows: 750W microwave treatment 4 minutes.
Select the circular glass steel culturing jar of diameter 2m, depth 0.9m as Cultivation container in step (1).
In step (2), by Cultivation container shift to an earlier date 2 days disinfection treatment (disinfection treatment of 10ppm potassium permanganate) inject no dirt afterwards
Contaminate well water, depth of water 0.7m.
The specific method of step (3) is: first setting up net cage in Cultivation container, selects 4 days C. guichenoti spray seedlings of membrane
It is put into raising in cage, 2500 tails of density/m3, 20 DEG C of fry rearing water temperature, feed daily 4 times, feed fairy shrimp within the 1st~10 day
Ten thousand tail of 2.5g/ feeds ten thousand tail of fairy shrimp 4.5g/ for 11~20 days；It is fry is point dilute and to be transferred to Cultivation container from net cage straight after 10 days
Connect cultivation, 1200 tails of density/m3, reach 2cm or so within 20 days, using feeding after the water earthworm disinfection minced, by fry after 30 days
Divide dilute, 700 tails of density/m3, reach 4cm or so within 60 days, made after hereafter being mixed using soft-shelled turtle material, water earthworm according to mass ratio 1:3
It is fed as dough.Net cage aperture is less than 1.0mm, and the size of net cage is 1.4m*1.4m*0.6m.It feeds 4 times daily, the time is
8:00,12:00,18:00,24:00.The water earthworm size minced is less than 1mm, and water earthworm disinfection is impregnated using 5ppm povidone iodine
30 minutes；The protein quality content of soft-shelled turtle material is greater than 45%.
Comparative example 3
A kind of high viability breeding method of C. guichenoti fry, comprising the following steps:
(1) selection of Cultivation container；
(2) water quality management: pollution-free well water is educated as cultivation water source miniflow water planting, is aerated using oxygen increasing pump whole day；
(3) fry is put in a suitable place to breed, and different phase uses different bait feedings.
Select the circular glass steel culturing jar of diameter 2m, depth 0.9m as Cultivation container in step (1).
In step (2), by Cultivation container shift to an earlier date 2 days disinfection treatment (disinfection treatment of 10ppm potassium permanganate) inject no dirt afterwards
Contaminate well water, depth of water 0.7m.
The specific method of step (3) is: first setting up net cage in Cultivation container, selects 4 days C. guichenoti spray seedlings of membrane
It is put into raising in cage, 2500 tails of density/m3, 20 DEG C of fry rearing water temperature, feed daily 4 times, feed fairy shrimp within the 1st~10 day
Ten thousand tail of 2.5g/ feeds ten thousand tail of fairy shrimp 4.5g/ for 11~20 days；It is fry is point dilute and to be transferred to Cultivation container from net cage straight after 10 days
Connect cultivation, 1200 tails of density/m3, reach 2cm or so within 20 days, using feeding after the water earthworm disinfection minced, by fry after 30 days
Divide dilute, 700 tails of density/m3, reach 4cm or so within 60 days, made after hereafter being mixed using soft-shelled turtle material, water earthworm according to mass ratio 1:3
It is fed as dough.Net cage aperture is less than 1.0mm, and the size of net cage is 1.4m*1.4m*0.6m.It feeds 4 times daily, the time is
8:00,12:00,18:00,24:00.The water earthworm size minced is less than 1mm, and water earthworm disinfection is impregnated using 5ppm povidone iodine
30 minutes；The protein quality content of soft-shelled turtle material is greater than 45%.
Test example
Be utilized respectively during in July, 2018~2019 year June the method for Examples 1 to 3 or comparative example 1~3 simultaneously into
Row C. guichenoti is cultivated, and statistics puts mantissa's (according to predetermined breeding density) and harvest mantissa in a suitable place to breed, is calculated survival rate, be the results are shown in Table 1.
Table 1. is cultivated situation and is compared
| Put mantissa (tail) in a suitable place to breed | It harvests mantissa (tail) | Survival rate (%) | |
| Embodiment 1 | 5652 | 5387 | 95.3 |
| Embodiment 2 | 5024 | 4798 | 95.5 |
| Embodiment 3 | 5495 | 5319 | 96.8 |
| Comparative example 1 | 5495 | 4698 | 85.5 |
| Comparative example 2 | 5495 | 4407 | 80.2 |
| Comparative example 3 | 5495 | 3313 | 60.3 |
As shown in Table 1, the method for Examples 1 to 3 cultivates the survival rate height of C. guichenoti.Comparative example 1 uses Serratia
It is prepared by bacterium ATCC14756 (being purchased from Shanghai North Connaught biological technology CO., LTD.) substitution grignard sand thunder bacterium ACCC01695, comparative example 2
Cerous sulfate is omitted when water-bed material, comparative example 3 is not laid with water-bed material at the bottom, and the survival rate of C. guichenoti is decreased obviously,
Illustrate that grignard sand thunder bacterium metabolite improves with specificity, close bacterial strain replacement gained metabolite C. guichenoti immunity
Significant lower to its immunity raising degree, the synergistic purifications water body such as cerous sulfate and polymeric aluminium ferrum silicate in water-bed material mentions
The survival rate of high C. guichenoti.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (9)
1. a kind of high viability breeding method of C. guichenoti fry, which comprises the following steps:
(1) selection of Cultivation container；
(2) water quality management: it is laid with one layer of water-bed material at the bottom, pollution-free well water is educated as cultivation water source miniflow water planting, used
Oxygen increasing pump whole day aeration；
(3) fry is put in a suitable place to breed, and different phase uses different bait feedings；
Wherein, the water-bed material is husky using modification infusorial earth load aggregation aluminium iron silicate, cerous sulfate, sodium humate, grignard
Thunder bacterium metabolite and obtain, the grignard sand thunder bacterium metabolite be grignard sand thunder bacterium ACCC01695 is activated, inoculation,
Fermentation obtains.
2. breeding method according to claim 1, which is characterized in that the thickness of water-bed material is about 5~8cm.
3. breeding method according to claim 1, which is characterized in that the preparation method of the grignard sand thunder bacterium metabolite
It is as follows: to be inoculated in seed culture medium after grignard sand thunder bacterium ACCC01695 activation, 28~31 DEG C are cultivated 12~15 hours, and bacterium is obtained
Strain seed liquor；Bacterial strain seed liquor is taken to be inoculated in fermentation medium, 27~31 DEG C, 180~200rpm/min shaken cultivation, from
The heart collects fermented supernatant fluid, precipitates to get a kind of grignard sand thunder bacterium metabolite；Wherein, the formula of seed culture medium are as follows: junket
8~12g of peptone, 6~9g of soluble starch, 5~8g of dextrin, 3~5g of sodium chloride, ammonium acetate 2~4g, FeSO40.2~
0.3g, CaCl23~4g, 900~1100mL, pH=6.8~7.2,121 DEG C of distilled water sterilize 20~30 minutes；Fermented and cultured
The formula of base are as follows: 4~6g of yeast extract,13C flag sucrose 0.2~0.3g, MgSO4·7H2O 5~8g, NaNO35~8g, KCl
8~12g, 900~1100mL, pH=6.8~7.2,121 DEG C of distilled water sterilize 20~30 minutes；13Knot when C flag sucrose exhausts
Beam culture.
4. breeding method according to claim 1, which is characterized in that the modification infusorial earth the preparation method is as follows: with
Parts by weight meter 1 part of neopelex is added in the water of 5~8 times of weight, then stirring and dissolving is heated with stirring to 70
~80 DEG C, 2~3 parts of activated diatomaceous earths are added, are stirred 3~4 hours under nitrogen atmosphere, filter, washing is drying to obtain.
5. breeding method according to claim 1, which is characterized in that it is described the bottom material the preparation method is as follows: with weight
Part meter is measured, by 1 part of polymeric aluminium ferrum silicate, 0.1~0.2 part of cerous sulfate, 3~4 parts of sodium humates, 0.3~0.4 part of grignard sand thunder bacterium
Metabolite pours into 15~20 parts of dehydrated alcohols, first time supersonic oscillations, abundant ball milling, and 8~10 parts of modifications are then added
Diatomite, second of supersonic oscillations, microwave treatment, third time supersonic oscillations, ethyl alcohol are volatilized naturally to get the water-bed material
Material.
6. breeding method according to claim 1, which is characterized in that select diameter 2m, 0.8~1m of depth in step (1)
Circular glass steel culturing jar as Cultivation container.
7. breeding method according to claim 1, which is characterized in that the specific method of step (3) is: first in Cultivation container
Middle erection net cage selects 4~5 days C. guichenoti spray seedlings of membrane to be put into raising in cage, 2000~3000 tails of density/m3, fry
18~22 DEG C of water temperature are cultivated, feeds daily 4 times, feeds within the 1st~10 day 2~3g/ of fairy shrimp, ten thousand tail, feed fairy shrimp within 11~20 days
4~5g/, ten thousand tail；Fry is point dilute and be transferred to Cultivation container from net cage and directly cultivate, 1000~1500 tails of density/m after 10 days3,
Reach 2cm or so within 20 days, is fed after being sterilized using the water earthworm minced, it is after 30 days that fry point is dilute, 500~800 tail of density/
m3, reach 4cm or so within 60 days, production dough is fed after hereafter mixing using soft-shelled turtle material, water earthworm according to mass ratio 1:2~4.
8. breeding method according to claim 1, which is characterized in that the net cage aperture is less than 1.0mm, the size of net cage
For 1.4m*1.4m*0.6m.
9. breeding method according to claim 1, which is characterized in that the water earthworm size minced is less than 1mm, water earthworm
Earthworm disinfection is impregnated 30 minutes using 5ppm povidone iodine；The protein quality content of soft-shelled turtle material is greater than 45%.
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| CN116349624B (en) * | 2023-03-10 | 2024-10-22 | 中国长江三峡集团有限公司中华鲟研究所 | Batch cultivation method for round-mouth copper fish fries |
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