CN108794205A - A kind of prevention and control vegetable insect disease type biological organic fertilizer and preparation method thereof - Google Patents
A kind of prevention and control vegetable insect disease type biological organic fertilizer and preparation method thereof Download PDFInfo
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- CN108794205A CN108794205A CN201810937501.0A CN201810937501A CN108794205A CN 108794205 A CN108794205 A CN 108794205A CN 201810937501 A CN201810937501 A CN 201810937501A CN 108794205 A CN108794205 A CN 108794205A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The present invention discloses a kind of prevention and control vegetable insect disease type biological organic fertilizer and preparation method thereof, belongs to technical field of biological fertilizer.The fertilizer is made of fish products processing fent, Brewery Wastewater Treatment sludge, fowl and animal excrement biogas slurry, the fibert dregs of rice, potassium fulvate, soil conditioner, complex micro organism fungicide, poly-aspartate and chitosan-silicon dioxide nano-particle according to certain weight ratio.The agricultural wastes such as the Fertilizer application fish products processing fent, organic nutrient substance is provided for vegetables, add the microbial bacterial agents such as bacillus, effectively inhibit the harmful microbes growth such as fungi, bacterium, virus in soil, effectively prevent the generation of various pest and disease damages in vegetables, this fertilizer is also equipped with promotion yield of vegetables simultaneously, and improvement soil texture adjusts soil acidity or alkalinity, improves the functions such as soil water regime.
Description
Technical field
The present invention relates to technical field of biological fertilizer, specially a kind of prevention and control vegetable insect disease type biological organic fertilizer and its system
Preparation Method.
Background technology
Vegetables are one of essential food in people's diet.Vegetables can provide a variety of dimension lifes necessary to human body
The nutriments such as element and minerals.It is counted according to international food and agricultural organization's nineteen ninety, the 90% of VC needed by human, the 60% of VA comes from vegetable
Dish.It is healthy and effective ingredient recognised by people in addition, there is diversified phytochemicals in vegetables, fruit at present
The many kinds of substance that nutrient in vegetable can effectively prevent chronic, degenerative disease is being found by people.The battalion of vegetables
Foster substance includes mainly protein, minerals, vitamin etc., and the content of these substances is higher, and the nutritive value of vegetables is also higher.
In addition, the content of the moisture and dietary fiber in vegetables is also important Nutrition quality indicator.In general, moisture is high, diet
The fresh and tender degree of the few vegetables of fiber is preferable, and edible value is also higher.But from the perspective of health care, dietary fiber is also that one kind must
Indispensable nutrient.The nutrient of vegetables can not be underestimated, and nineteen ninety world food and agricultural organization counts needed by human ascorbic
90%, the 60% of vitamin A is all from vegetables, it is seen that vegetables are huge to the contribution of human health.In addition, there are many being gone back in vegetables
Phytochemicals is the putative ingredient beneficial to health, such as carotenoid, two propen compounds, methyl vulcanization
Object etc. is closed, many vegetables also contain unique trace element, have special health-care efficacy to human body, such as the tomato in tomato
Prostaglandin etc. in red pigment, onion.
Currently, vegetable insect disease is an important factor for influencing China's yield of vegetables and security quality, can generally to cause vegetables
10% one the 30% of production loss；In plant disease epidemic, loss is more serious, is lost up to 60%-70% or more even absolutely according to analysis
Production.Chemical insecticide can effectively administer vegetable insect disease, but its is of high cost, efficacy time is short, long in the vegetable surface residence time,
It is difficult to decompose, easily causes people and animals' illness and environmental pollution.With the continuous promotion of quality of life, people are for quality of vegetable
Requirement it is higher and higher,.Therefore it is badly in need of developing a kind of ecological environment-friendly type fertilizer of effective prevention and control vegetable insect disease.
Invention content
To solve problems of the prior art, the present invention provide a kind of prevention and control vegetable insect disease type biological organic fertilizer and
Preparation method.The present invention is main with fish products processing fent, Brewery Wastewater Treatment sludge and fowl and animal excrement etc.
Raw material is aided with microbial bacterial agent and soil conditioner etc., on the one hand solves the problems, such as vegetables nutrient supply, on the other hand effectively
Inhibit the growth of the harmful microbes such as fungi, bacterium, the virus in soil, to the generation of effective prevention and control vegetable insect disease.
The technical solution adopted in the present invention is：
A kind of prevention and control vegetable insect disease type biological organic fertilizer, which is characterized in that it is made of the raw material of following parts by weight：Fish
400-600 parts of product processing fent, 200-400 parts of Brewery Wastewater Treatment sludge, 100-200 parts of fowl and animal excrement biogas slurry, hazel
Sub- dregs of rice 40-60 parts, 20-40 parts of potassium fulvate, 10-20 parts of soil conditioner, 1-5 parts of complex micro organism fungicide, poly-aspartate
1-5 parts and 1-3 parts of chitosan-silicon dioxide nano-particle.
The soil conditioner is oyster shell powder, dolomite dust and medical stone powder according to 3:5:8 ratio mixes.
The complex micro organism fungicide is prepared by following methods：By aspergillus niger, Bacillus cercus, withered grass gemma
It after bacillus and saccharomycete activation cultivates 15-20 hours at 25 DEG C in liquid seed culture medium respectively, is then connect according to 30 ‰
Kind amount is transferred in sterilized big tank culture medium, after being cultivated 25 hours at 30 DEG C, by aspergillus niger, Bacillus cercus, withered grass bud
Spore bacillus and saccharomycete are according to mass ratio 1:3:3:5 compound.
The chitosan-silicon dioxide nano-particle is prepared using following methods：
（1）1g chitosans are added in the acetum that 100mL mass concentrations are 3%, magnetic agitation 12h, obtain chitosan
Solution；
（2）By hexamethylene, surfactant TritonX-100 and n-hexyl alcohol by volume 4: 1: 1 be uniformly mixed and 3 times of weights are added
After the deionized water stirring 10min of amount, step is added（1）Gained chitosan solution, NaOH solution is added is by system adjusting PH
7, control water phase and the ratio between the amount of substance of surfactant TritonX-100 maintain 10: 1；After stirring 1h, silanization is tried
Agent TEOS and ammonium hydroxide are added to by 1.7: 1 volume ratio in solution, and after stirring evenly, reaction continues for 24 hours；After the completion of reaction, add
Enter acetone to be demulsified, particle is collected by centrifugation, washs nano particle, shell obtained with acetone, absolute ethyl alcohol, deionized water successively
Glycan-Nano particles of silicon dioxide.
The preparation method of prevention and control vegetable insect disease type biological organic fertilizer of the present invention, it includes the following steps：
（1）1g chitosans are added in the acetum that 100mL mass concentrations are 3%, magnetic agitation 12h, obtain chitosan
Solution, it is spare；
（2）By hexamethylene, surfactant TritonX-100 and n-hexyl alcohol by volume 4: 1: 1 be uniformly mixed and 3 times of weights are added
After the deionized water stirring 10min of amount, step is added（1）Gained chitosan solution, NaOH solution is added is by system adjusting PH
7, control water phase and the ratio between the amount of substance of surfactant TritonX-100 maintain 10: 1；After stirring 1h, silanization is tried
Agent TEOS and ammonium hydroxide are added to by 1.7: 1 volume ratio in solution, and after stirring evenly, reaction continues for 24 hours；After the completion of reaction, add
Enter acetone to be demulsified, particle is collected by centrifugation, washs nano particle, shell obtained with acetone, absolute ethyl alcohol, deionized water successively
Glycan-Nano particles of silicon dioxide, it is spare；
（3）After aspergillus niger, Bacillus cercus, bacillus subtilis and saccharomycete are activated respectively in liquid seed culture medium
It cultivates at 25 DEG C 15-20 hours, is then transferred in sterilized big tank culture medium according to 30 ‰ inoculum concentration, 25 are cultivated at 30 DEG C
After hour, by aspergillus niger, Bacillus cercus, bacillus subtilis and saccharomycete according to mass ratio 1:3:3:5 compoundings are made micro-
Bacteria agent is spare；
（4）Fish products processing fent and Brewery Wastewater Treatment sludge are uniformly mixed according to formula ratio, then to mixing
According to solid-liquid ratio 1kg in object：The MgCl of 0.4M is added in 30ml2Solution is stirred continuously, and is added after stirring 60min and is accounted for solid material
The sodium hydroxide solution of the 3.0mol/L of weight ratio 2% continues to stir 20min, and mixed material is obtained after then being dried in baking oven
Material A is continued to be placed in Muffle furnace and is roasted 10 hours at 450 ~ 650 DEG C under conditions of closed isolation air by A, to be cooled
To room temperature, it is ground into the powder material B not less than 100 mesh；
（5）Fowl and animal excrement biogas slurry is taken, flocculant is added thereto, activated carbon is added and is sufficiently stirred, is then removed by filtration precipitation
Biogas slurry that object obtains that treated；
（6）By step（5）Prepared treated biogas slurry, the fibert dregs of rice, complex micro organism fungicide, poly-aspartate and shell are poly-
Sugar-Nano particles of silicon dioxide is mixed thoroughly, and stirring is primary daily after 5 days, 5 days for first anaerobic fermentation, continuously stirs 20-25
It, tunning dries pulverizing is obtained powder C by control fermentation central temperature after fermentation at 55 ~ 65 DEG C；
（7）Powder material B, powder C and potassium fulvate, soil conditioner are uniformly mixed, comminutor is sent into and is granulated, dispensed after dry
Product of the present invention.
Step（5）The addition of middle flocculant accounts for the 6-8% of biogas slurry volume.
Step（5）Middle activated carbon addition accounts for the 12-15% of biogas slurry weight.
Aspergillus niger, Bacillus cercus, bacillus subtilis and saccharomycete used herein are commercially available gained, used
Flocculant is purchased from Wuhan Lv Yuan water-purifying materials Co., Ltd PAM series of products.The surfactant TritonX-100 is poly- second
Glycol octyl phenyl ether is a kind of nonionic surface active agent.The silylating reagent TEOS is ethyl orthosilicate.
The beneficial effects of the invention are as follows：
1, the present invention is based on fish products processing fent, Brewery Wastewater Treatment sludge, fowl and animal excrement and fibert dregs of rice etc.
Raw material is wanted, provides comprehensive organic nutrient substance for vegetables, while efficiently using waste, environmental protection；Processed fish production
Product leftover bits and pieces and Brewery Wastewater Treatment sludge contain the substances such as biomass carbon, and having improves soil physico-chemical property, delays fertilizer
Expect nutrients release, reduce the loss of fertilizer and soil nutrient and improves microbial activity, the application effect of retain water and nutrients, while
Good growing environment is provided for edaphon and vegetable growth；With the use of oyster shell powder, dolomite dust and wheat meal
The soil conditioners such as mountain flour can effectively improve soil texture, reduce soil alkaline harm, adjust soil acidity or alkalinity, improve soil
Water regime or repairing polluted soil etc.；
2, inventor by verifying repeatedly, and preferably aspergillus niger, Bacillus cercus, bacillus subtilis and saccharomycete are made
For the effective microbe of the present invention, and the optimum proportioning of each microbial inoculum has been obtained；Maximum of the saccharomycete as fruits and vegetables biocontrol bacterium
Advantage, which is it, can be bred using nutrition rapidly in relatively dry fruit and vegetable surfaces existence, and is influenced by insecticide small；It is black
Aspergillus, wax-like bud pole bacterium and bacillus subtilis can control the soil-borne fungus and bacterial disease of various vegetables and crops
Evil, and have certain growth-promoting functions to plant growth, its resistance is strong, can also generate a variety of antibacterial materials.Addition simultaneously
Chitosan-silicon dioxide nano-particle can act synergistically with microorganism, be generated after promotion complex micro organism fungicide fermentation various
The activity of enzyme and antibacterial material, and promote active material being uniformly distributed in the soil, effectively inhibit the hair of vegetable insect disease
It is raw；It can promote absorption of the vegetables to nutriments such as minerals simultaneously, promote vegetable growth, improve yield of vegetables.
Specific implementation mode
Technical scheme of the present invention is described further with reference to specific embodiment, but not limited to this.
Embodiment 1
A kind of prevention and control vegetable insect disease type biological organic fertilizer, which is characterized in that it is made of the raw material of following parts by weight：Fish
400 parts of product processing fent, 200 parts of Brewery Wastewater Treatment sludge, 100 parts of fowl and animal excrement biogas slurry, 40 parts of the fibert dregs of rice, yellow corruption
20 parts of sour potassium, 10 parts of soil conditioner, 1 part of complex micro organism fungicide, 1 part of poly-aspartate and chitosan-silicon dioxide nanometer
1 part of particle.
The soil conditioner is oyster shell powder, dolomite dust and medical stone powder according to 3:5:8 ratio mixes.
The complex micro organism fungicide is prepared by following methods：By aspergillus niger, Bacillus cercus, withered grass gemma
Cultivate 15 hours at 25 DEG C in liquid seed culture medium respectively after bacillus and saccharomycete activation, then according to 30 ‰ inoculum concentration
It is transferred in sterilized big tank culture medium, after being cultivated 25 hours at 30 DEG C, by aspergillus niger, Bacillus cercus, bacillus subtilis
Bacterium and saccharomycete are according to mass ratio 1:3:3:5 compound.
The chitosan-silicon dioxide nano-particle is prepared using following methods：
（1）1g chitosans are added in the acetum that 100mL mass concentrations are 3%, magnetic agitation 12h, obtain chitosan
Solution；
（2）By hexamethylene, surfactant TritonX-100 and n-hexyl alcohol by volume 4: 1: 1 be uniformly mixed and 3 times of weights are added
After the deionized water stirring 10min of amount, step is added（1）Gained chitosan solution, NaOH solution is added is by system adjusting PH
7, control water phase and the ratio between the amount of substance of surfactant TritonX-100 maintain 10: 1；After stirring 1h, silanization is tried
Agent TEOS and ammonium hydroxide are added to by 1.7: 1 volume ratio in solution, and after stirring evenly, reaction continues for 24 hours；After the completion of reaction, add
Enter acetone to be demulsified, particle is collected by centrifugation, washs nano particle, shell obtained with acetone, absolute ethyl alcohol, deionized water successively
Glycan-Nano particles of silicon dioxide.
The preparation method of prevention and control vegetable insect disease type biological organic fertilizer of the present invention, which is characterized in that it includes following
Step：
（1）1g chitosans are added in the acetum that 100mL mass concentrations are 3%, magnetic agitation 12h, obtain chitosan
Solution, it is spare；
（2）By hexamethylene, surfactant TritonX-100 and n-hexyl alcohol by volume 4: 1: 1 be uniformly mixed and 3 times of weights are added
After the deionized water stirring 10min of amount, step is added（1）Gained chitosan solution, NaOH solution is added is by system adjusting PH
7, control water phase and the ratio between the amount of substance of surfactant TritonX-100 maintain 10: 1；After stirring 1h, silanization is tried
Agent TEOS and ammonium hydroxide are added to by 1.7: 1 volume ratio in solution, and after stirring evenly, reaction continues for 24 hours；After the completion of reaction, add
Enter acetone to be demulsified, particle is collected by centrifugation, washs nano particle, shell obtained with acetone, absolute ethyl alcohol, deionized water successively
Glycan-Nano particles of silicon dioxide, it is spare；
（3）After aspergillus niger, Bacillus cercus, bacillus subtilis and saccharomycete are activated respectively in liquid seed culture medium
It cultivates at 25 DEG C 15-20 hours, is then transferred in sterilized big tank culture medium according to 30 ‰ inoculum concentration, 25 are cultivated at 30 DEG C
After hour, by aspergillus niger, Bacillus cercus, bacillus subtilis and saccharomycete according to mass ratio 1:3:3:5 compoundings are made micro-
Bacteria agent is spare；
（4）Fish products processing fent and Brewery Wastewater Treatment sludge are uniformly mixed according to formula ratio, then to mixing
According to solid-liquid ratio 1kg in object：The MgCl of 0.4M is added in 30ml2Solution is stirred continuously, and is added after stirring 60min and is accounted for solid material
The sodium hydroxide solution of the 3.0mol/L of weight ratio 2% continues to stir 20min, and mixed material is obtained after then being dried in baking oven
Material A is continued to be placed in Muffle furnace and is roasted 10 hours at 450 DEG C under conditions of closed isolation air, is cooled to room by A
Wen Hou is ground into the powder material B not less than 100 mesh；
（5）Fowl and animal excrement biogas slurry is taken, flocculant is added thereto, activated carbon is added and is sufficiently stirred, is then removed by filtration precipitation
Biogas slurry that object obtains that treated；
（6）By step（5）Prepared treated biogas slurry, the fibert dregs of rice, complex micro organism fungicide, poly-aspartate and shell are poly-
Sugar-Nano particles of silicon dioxide is mixed thoroughly, and stirring is primary daily after 5 days, 5 days for first anaerobic fermentation, continuously stirs 20 days,
Tunning dries pulverizing is obtained powder C by control fermentation central temperature after fermentation at 55 DEG C；
（7）Powder material B, powder C and potassium fulvate, soil conditioner are uniformly mixed, comminutor is sent into and is granulated, dispensed after dry
Product of the present invention.
Step（5）The addition of middle flocculant accounts for the 6% of biogas slurry volume.
Step（5）Middle activated carbon addition accounts for the 12% of biogas slurry weight.
Embodiment 2
A kind of prevention and control vegetable insect disease type biological organic fertilizer of the present invention, which is characterized in that it is by the raw material system of following parts by weight
At：600 parts of fish products processing fent, 400 parts of Brewery Wastewater Treatment sludge, 200 parts of fowl and animal excrement biogas slurry, the fibert dregs of rice 60
Part, 40 parts of potassium fulvate, 20 parts of soil conditioner, 5 parts of complex micro organism fungicide, 5 parts of poly-aspartate and chitosan-titanium dioxide
3 parts of silicon nano.
The soil conditioner is oyster shell powder, dolomite dust and medical stone powder according to 3:5:8 ratio mixes.
The complex micro organism fungicide is prepared by following methods：By aspergillus niger, Bacillus cercus, withered grass gemma
Cultivate 20 hours at 25 DEG C in liquid seed culture medium respectively after bacillus and saccharomycete activation, then according to 30 ‰ inoculum concentration
It is transferred in sterilized big tank culture medium, after being cultivated 25 hours at 30 DEG C, by aspergillus niger, Bacillus cercus, bacillus subtilis
Bacterium and saccharomycete are according to mass ratio 1:3:3:5 compound.
The chitosan-silicon dioxide nano-particle is prepared using following methods：
（1）1g chitosans are added in the acetum that 100mL mass concentrations are 3%, magnetic agitation 12h, obtain chitosan
Solution；
（2）By hexamethylene, surfactant TritonX-100 and n-hexyl alcohol by volume 4: 1: 1 be uniformly mixed and 3 times of weights are added
After the deionized water stirring 10min of amount, step is added（1）Gained chitosan solution, NaOH solution is added is by system adjusting PH
7, control water phase and the ratio between the amount of substance of surfactant TritonX-100 maintain 10: 1；After stirring 1h, silanization is tried
Agent TEOS and ammonium hydroxide are added to by 1.7: 1 volume ratio in solution, and after stirring evenly, reaction continues for 24 hours；After the completion of reaction, add
Enter acetone to be demulsified, particle is collected by centrifugation, washs nano particle, shell obtained with acetone, absolute ethyl alcohol, deionized water successively
Glycan-Nano particles of silicon dioxide.
The preparation method of prevention and control vegetable insect disease type biological organic fertilizer of the present invention, which is characterized in that it includes following
Step：
（1）1g chitosans are added in the acetum that 100mL mass concentrations are 3%, magnetic agitation 12h, obtain chitosan
Solution, it is spare；
（2）By hexamethylene, surfactant TritonX-100 and n-hexyl alcohol by volume 4: 1: 1 be uniformly mixed and 3 times of weights are added
After the deionized water stirring 10min of amount, step is added（1）Gained chitosan solution, NaOH solution is added is by system adjusting PH
7, control water phase and the ratio between the amount of substance of surfactant TritonX-100 maintain 10: 1；After stirring 1h, silanization is tried
Agent TEOS and ammonium hydroxide are added to by 1.7: 1 volume ratio in solution, and after stirring evenly, reaction continues for 24 hours；After the completion of reaction, add
Enter acetone to be demulsified, particle is collected by centrifugation, washs nano particle, shell obtained with acetone, absolute ethyl alcohol, deionized water successively
Glycan-Nano particles of silicon dioxide, it is spare；
（3）After aspergillus niger, Bacillus cercus, bacillus subtilis and saccharomycete are activated respectively in liquid seed culture medium
It cultivates at 25 DEG C 15-20 hours, is then transferred in sterilized big tank culture medium according to 30 ‰ inoculum concentration, 25 are cultivated at 30 DEG C
After hour, by aspergillus niger, Bacillus cercus, bacillus subtilis and saccharomycete according to mass ratio 1:3:3:5 compoundings are made micro-
Bacteria agent is spare；
（4）Fish products processing fent and Brewery Wastewater Treatment sludge are uniformly mixed according to formula ratio, then to mixing
According to solid-liquid ratio 1kg in object：The MgCl of 0.4M is added in 30ml2Solution is stirred continuously, and is added after stirring 60min and is accounted for solid material
The sodium hydroxide solution of the 3.0mol/L of weight ratio 2% continues to stir 20min, and mixed material is obtained after then being dried in baking oven
Material A is continued to be placed in Muffle furnace and is roasted 10 hours at 650 DEG C under conditions of closed isolation air, is cooled to room by A
Wen Hou is ground into the powder material B not less than 100 mesh；
（5）Fowl and animal excrement biogas slurry is taken, flocculant is added thereto, activated carbon is added and is sufficiently stirred, is then removed by filtration precipitation
Biogas slurry that object obtains that treated；
（6）By step（5）Prepared treated biogas slurry, the fibert dregs of rice, complex micro organism fungicide, poly-aspartate and shell are poly-
Sugar-Nano particles of silicon dioxide is mixed thoroughly, and stirring is primary daily after 5 days, 5 days for first anaerobic fermentation, continuously stirs 25 days,
Tunning dries pulverizing is obtained powder C by control fermentation central temperature after fermentation at 65 DEG C；
（7）Powder material B, powder C and potassium fulvate, soil conditioner are uniformly mixed, comminutor is sent into and is granulated, dispensed after dry
Product of the present invention.
Step（5）The addition of middle flocculant accounts for the 8% of biogas slurry volume.
Step（5）Middle activated carbon addition accounts for the 15% of biogas slurry weight.
Embodiment 3
A kind of prevention and control vegetable insect disease type biological organic fertilizer, which is characterized in that it is made of the raw material of following parts by weight：Fish
500 parts of product processing fent, 300 parts of Brewery Wastewater Treatment sludge, 150 parts of fowl and animal excrement biogas slurry, 50 parts of the fibert dregs of rice, yellow corruption
30 parts of sour potassium, 15 parts of soil conditioner, 3 parts of complex micro organism fungicide, 3 parts of poly-aspartate and chitosan-silicon dioxide nanometer
2 parts of particle.
The soil conditioner is oyster shell powder, dolomite dust and medical stone powder according to 3:5:8 ratio mixes.
The complex micro organism fungicide is prepared by following methods：By aspergillus niger, Bacillus cercus, withered grass gemma
Cultivate 17 hours at 25 DEG C in liquid seed culture medium respectively after bacillus and saccharomycete activation, then according to 30 ‰ inoculum concentration
It is transferred in sterilized big tank culture medium, after being cultivated 25 hours at 30 DEG C, by aspergillus niger, Bacillus cercus, bacillus subtilis
Bacterium and saccharomycete are according to mass ratio 1:3:3:5 compound.
The chitosan-silicon dioxide nano-particle is prepared using following methods：
（1）1g chitosans are added in the acetum that 100mL mass concentrations are 3%, magnetic agitation 12h, obtain chitosan
Solution；
（2）By hexamethylene, surfactant TritonX-100 and n-hexyl alcohol by volume 4: 1: 1 be uniformly mixed and 3 times of weights are added
After the deionized water stirring 10min of amount, step is added（1）Gained chitosan solution, NaOH solution is added is by system adjusting PH
7, control water phase and the ratio between the amount of substance of surfactant TritonX-100 maintain 10: 1；After stirring 1h, silanization is tried
Agent TEOS and ammonium hydroxide are added to by 1.7: 1 volume ratio in solution, and after stirring evenly, reaction continues for 24 hours；After the completion of reaction, add
Enter acetone to be demulsified, particle is collected by centrifugation, washs nano particle, shell obtained with acetone, absolute ethyl alcohol, deionized water successively
Glycan-Nano particles of silicon dioxide.
The preparation method of prevention and control vegetable insect disease type biological organic fertilizer of the present invention, which is characterized in that it includes following
Step：
（1）1g chitosans are added in the acetum that 100mL mass concentrations are 3%, magnetic agitation 12h, obtain chitosan
Solution, it is spare；
（2）By hexamethylene, surfactant TritonX-100 and n-hexyl alcohol by volume 4: 1: 1 be uniformly mixed and 3 times of weights are added
After the deionized water stirring 10min of amount, step is added（1）Gained chitosan solution, NaOH solution is added is by system adjusting PH
7, control water phase and the ratio between the amount of substance of surfactant TritonX-100 maintain 10: 1；After stirring 1h, silanization is tried
Agent TEOS and ammonium hydroxide are added to by 1.7: 1 volume ratio in solution, and after stirring evenly, reaction continues for 24 hours；After the completion of reaction, add
Enter acetone to be demulsified, particle is collected by centrifugation, washs nano particle, shell obtained with acetone, absolute ethyl alcohol, deionized water successively
Glycan-Nano particles of silicon dioxide, it is spare；
（3）After aspergillus niger, Bacillus cercus, bacillus subtilis and saccharomycete are activated respectively in liquid seed culture medium
It cultivates at 25 DEG C 17 hours, is then transferred in sterilized big tank culture medium according to 30 ‰ inoculum concentration, it is small that 25 are cultivated at 30 DEG C
Shi Hou, by aspergillus niger, Bacillus cercus, bacillus subtilis and saccharomycete according to mass ratio 1:3:3:Micro- life is made in 5 compoundings
Object microbial inoculum is spare；
（4）Fish products processing fent and Brewery Wastewater Treatment sludge are uniformly mixed according to formula ratio, then to mixing
According to solid-liquid ratio 1kg in object：The MgCl of 0.4M is added in 30ml2Solution is stirred continuously, and is added after stirring 60min and is accounted for solid material
The sodium hydroxide solution of the 3.0mol/L of weight ratio 2% continues to stir 20min, and mixed material is obtained after then being dried in baking oven
Material A is continued to be placed in Muffle furnace and is roasted 10 hours at 550 DEG C under conditions of closed isolation air, is cooled to room by A
Wen Hou is ground into the powder material B not less than 100 mesh；
（5）Fowl and animal excrement biogas slurry is taken, flocculant is added thereto, activated carbon is added and is sufficiently stirred, is then removed by filtration precipitation
Biogas slurry that object obtains that treated；
（6）By step（5）Prepared treated biogas slurry, the fibert dregs of rice, complex micro organism fungicide, poly-aspartate and shell are poly-
Sugar-Nano particles of silicon dioxide is mixed thoroughly, and stirring is primary daily after 5 days, 5 days for first anaerobic fermentation, continuously stirs 23 days,
Tunning dries pulverizing is obtained powder C by control fermentation central temperature after fermentation at 60 DEG C；
（7）Powder material B, powder C and potassium fulvate, soil conditioner are uniformly mixed, comminutor is sent into and is granulated, dispensed after dry
Product of the present invention.
Step（5）The addition of middle flocculant accounts for the 7% of biogas slurry volume.
Step（5）Middle activated carbon addition accounts for the 14% of biogas slurry weight.
Comparative example 1
A kind of prevention and control vegetable insect disease type biological organic fertilizer, which is characterized in that it is made of the raw material of following parts by weight：Fish
400 parts of product processing fent, 200 parts of Brewery Wastewater Treatment sludge, 100 parts of fowl and animal excrement biogas slurry, 40 parts of the fibert dregs of rice, yellow corruption
1 part of 20 parts of sour potassium, 10 parts of soil conditioner and poly-aspartate.
In the preparation method of above-mentioned fertilizer in addition to without containing the preparation for meeting microbial bacterial agent and addition, remaining same embodiment
1。
Comparative example 2
A kind of prevention and control vegetable insect disease type biological organic fertilizer, which is characterized in that it is made of the raw material of following parts by weight：Fish
400 parts of product processing fent, 200 parts of Brewery Wastewater Treatment sludge, 100 parts of fowl and animal excrement biogas slurry, 40 parts of the fibert dregs of rice, yellow corruption
1 part of 20 parts of sour potassium, 10 parts of soil conditioner, 1 part of complex micro organism fungicide and poly-aspartate.
In the preparation method of above-mentioned fertilizer in addition to preparation and addition without containing chitosan-silicon dioxide nano-particle,
Remaining same embodiment 1.
Fertilizer application is tested
Application examples 1
Test site：It chooses 6 pieces of Suixuan County vegetable cultivation base in Hubei Province experimental plot to be tested, experimental plot soil regime one
It causes, soil fertility is uniform.
For studying object：Celery, kind are Jin Nan realities celery No. 1, are planted in a manner of outdoor sowing plus covering with ground sheeting.
Experiment grouping：Experiment sets embodiment 1-3, comparative example 1-2 and 6 processing groups of blank example, and blank example is without using any
Fertilizer is grown naturally；The dosage and method of application all same of embodiment 1-3 and comparative example 1-2 fertilizer.Count celery pest and disease damage hair
Raw situation, statistical result are as shown in the table：
1 celery of table plants application effect
It can be seen that from the result in above-mentioned table using fertilizer prepared by 1-3 of the embodiment of the present invention, can effectively promote celery production
Amount promotes celery quality, the generation of effective its pest and disease damage of prevention and control.And lacked the comparative example 1 of complex micro organism fungicide, prevention and control
Disease pest ability is decreased obviously；The comparative example 2 of chitosan-silicon dioxide nano-particle is not added, and disease resistance ability is than comparative example 1
It is slightly good, but can not show a candle to the fertilizer efficiency of 1-3 of the embodiment of the present invention.Therefore, nutrient formula of the present invention is reasonable, is provided for celery abundant
Nutriment, effect of increasing production are apparent；The supplies such as scientific matching microbial bacterial agent and nano-particle simultaneously make fertilizer sterilization suppression
Bacterium is with obvious effects, the generation of effective prevention and control pest and disease damage.
Application examples 2
Test site：It chooses 6 pieces of Suixuan County vegetable cultivation base in Hubei Province experimental plot to be tested, experimental plot soil regime one
It causes, soil fertility is uniform.
For studying object：Chinese cabbage, kind are emerald green No. 3 of Beijing.
Experiment grouping：Experiment sets embodiment 1-3, comparative example 1-2 and 6 processing groups of blank example, and blank example is without using any
Fertilizer is grown naturally；The dosage and method of application all same of embodiment 1-3 and comparative example 1-2 fertilizer.Count Chinese cabbage pest and disease damage hair
Raw situation, statistical result are as shown in the table：
2 Chinese cabbage of table plants application effect
The fertilizer using 1-3 of the embodiment of the present invention is can be seen that by data in table, can effectively promote Chinese cabbage yield, while its
Downy mildew, soft rot, downy mildew incidence can be controlled in 5% hereinafter, having and significantly carrying with blank example compared with comparative example 1-2
It rises.Nutrient formula of the present invention is reasonable, and microbial bacterial agent selects science, while promoting Chinese cabbage yield, its disease pest of effective prevention and control
Harmful generation.
It should be noted that above-described embodiment is only the section Example for realizing the preferred embodiment of the present invention, Er Feiquan
Portion's embodiment.Obviously, it is based on the above embodiment of the present invention, those of ordinary skill in the art are not making creative work
Under the premise of other all embodiments for being obtained, should all belong to the scope of protection of the invention.
Claims (7)
1. a kind of prevention and control vegetable insect disease type biological organic fertilizer, which is characterized in that it is made of the raw material of following parts by weight：Fish
400-600 parts of class product processing fent, 200-400 parts of Brewery Wastewater Treatment sludge, 100-200 parts of fowl and animal excrement biogas slurry,
40-60 parts of the fibert dregs of rice, 20-40 parts of potassium fulvate, 10-20 parts of soil conditioner, 1-5 parts of complex micro organism fungicide, poly- asparagus fern ammonia
Sour 1-5 parts and 1-3 parts of chitosan-silicon dioxide nano-particle.
2. prevention and control vegetable insect disease type biological organic fertilizer according to claim 1, which is characterized in that the soil conditioner is
Oyster shell powder, dolomite dust and medical stone powder are according to 3:5:8 ratio mixes.
3. prevention and control vegetable insect disease type biological organic fertilizer according to claim 1, which is characterized in that the composite microbial bacteria
Agent is prepared by following methods：Exist respectively after aspergillus niger, Bacillus cercus, bacillus subtilis and saccharomycete are activated
It is cultivated at 25 DEG C in liquid seed culture medium 15-20 hours, is then transferred to sterilized big tank culture according to 30 ‰ inoculum concentration
In base, after being cultivated 25 hours at 30 DEG C, by aspergillus niger, Bacillus cercus, bacillus subtilis and saccharomycete according to mass ratio
1:3:3:5 compound.
4. prevention and control vegetable insect disease type biological organic fertilizer according to claim 1, which is characterized in that the chitosan-dioxy
SiClx nano-particle is prepared using following methods：
（1）1g chitosans are added in the acetum that 100mL mass concentrations are 3%, magnetic agitation 12h, obtain chitosan
Solution；
（2）By hexamethylene, surfactant TritonX-100 and n-hexyl alcohol by volume 4: 1: 1 be uniformly mixed and 3 times of weights are added
After the deionized water stirring 10min of amount, step is added（1）Gained chitosan solution, NaOH solution is added is by system adjusting PH
7, control water phase and the ratio between the amount of substance of surfactant TritonX-100 maintain 10: 1；After stirring 1h, silanization is tried
Agent TEOS and ammonium hydroxide are added to by 1.7: 1 volume ratio in solution, and after stirring evenly, reaction continues for 24 hours；After the completion of reaction, add
Enter acetone to be demulsified, particle is collected by centrifugation, washs nano particle, shell obtained with acetone, absolute ethyl alcohol, deionized water successively
Glycan-Nano particles of silicon dioxide.
5. according to the preparation method of any one of the claim 1-4 prevention and control vegetable insect disease type biological organic fertilizers, feature exists
In it includes the following steps：
（1）1g chitosans are added in the acetum that 100mL mass concentrations are 3%, magnetic agitation 12h, obtain chitosan
Solution, it is spare；
（2）By hexamethylene, surfactant TritonX-100 and n-hexyl alcohol by volume 4: 1: 1 be uniformly mixed and 3 times of weights are added
After the deionized water stirring 10min of amount, step is added（1）Gained chitosan solution, NaOH solution is added is by system adjusting PH
7, control water phase and the ratio between the amount of substance of surfactant TritonX-100 maintain 10: 1；After stirring 1h, silanization is tried
Agent TEOS and ammonium hydroxide are added to by 1.7: 1 volume ratio in solution, and after stirring evenly, reaction continues for 24 hours；After the completion of reaction, add
Enter acetone to be demulsified, particle is collected by centrifugation, washs nano particle, shell obtained with acetone, absolute ethyl alcohol, deionized water successively
Glycan-Nano particles of silicon dioxide, it is spare；
（3）After aspergillus niger, Bacillus cercus, bacillus subtilis and saccharomycete are activated respectively in liquid seed culture medium
It cultivates at 25 DEG C 15-20 hours, is then transferred in sterilized big tank culture medium according to 30 ‰ inoculum concentration, 25 are cultivated at 30 DEG C
After hour, by aspergillus niger, Bacillus cercus, bacillus subtilis and saccharomycete according to mass ratio 1:3:3:5 compoundings are made micro-
Bacteria agent is spare；
（4）Fish products processing fent and Brewery Wastewater Treatment sludge are uniformly mixed according to formula ratio, then to mixing
According to solid-liquid ratio 1kg in object：The MgCl of 0.4M is added in 30ml2Solution is stirred continuously, and is added after stirring 60min and is accounted for solid material
The sodium hydroxide solution of the 3.0mol/L of weight ratio 2% continues to stir 20min, and mixed material is obtained after then being dried in baking oven
Material A is continued to be placed in Muffle furnace and is roasted 10 hours at 450 ~ 650 DEG C under conditions of closed isolation air by A, to be cooled
To room temperature, it is ground into the powder material B not less than 100 mesh；
（5）Fowl and animal excrement biogas slurry is taken, flocculant is added thereto, activated carbon is added and is sufficiently stirred, is then removed by filtration precipitation
Biogas slurry that object obtains that treated；
（6）By step（5）Prepared treated biogas slurry and the fibert dregs of rice, complex micro organism fungicide, poly-aspartate, shell are poly-
Sugar-Nano particles of silicon dioxide is mixed thoroughly, and stirring is primary daily after 5 days, 5 days for first anaerobic fermentation, continuously stirs 20-25
It, tunning dries pulverizing is obtained powder C by control fermentation central temperature after fermentation at 55 ~ 65 DEG C；
（7）Powder material B, powder C and potassium fulvate, soil conditioner are uniformly mixed, comminutor is sent into and is granulated, dispensed after dry
Product of the present invention.
6. the preparation method of prevention and control vegetable insect disease type biological organic fertilizer according to claim 5, which is characterized in that step
（5）The addition of middle flocculant accounts for the 6-8% of biogas slurry volume.
7. according to the preparation method of prevention and control vegetable insect disease type biological organic fertilizer described in claim 5, which is characterized in that step（5）
Middle activated carbon addition accounts for the 12-15% of biogas slurry weight.
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