CN108553559A - A kind of vegetables and fruits extraction composition formula and preparation method for preventing type II diabetes - Google Patents
A kind of vegetables and fruits extraction composition formula and preparation method for preventing type II diabetes Download PDFInfo
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Abstract
The vegetables and fruits extraction composition formula and preparation method that the invention discloses a kind of for preventing type II diabetes, include the raw material components of following parts by weight：5~30 parts of longevity greens/mustard green, 4~20 parts of konjaku, 3~15 parts of sea-buckthorn, 2~10 parts to day fruit, 2 10 parts of pueraria lobata and 2 10 parts of Siraitia grosvenorii.A kind of vegetables and fruits plant extracts preparation method for preventing type II diabetes includes the following steps：S1, by clean longevity greens/mustard green, konjaku, sea-buckthorn, pueraria lobata, Siraitia grosvenorii and decladding to day fruit, low temperature dries spare；S2, longevity greens/mustard green is crushed, 80 100 mesh sieve is crossed, with 45% ethanol as solvent, solid-to-liquid ratio 1：10, fully stir evenly.Compared with prior art, a kind of apply in chronic metabolism of blood glucose disease areas is provided, it is composed of pure natural vegetables and fruits plant extracts, is without any side effects, added without external addition anti-corrosion, pigment chemical reagent, the vegetables and fruits plant extracts combination formula for preventing type II diabetes.
Description
Technical field
The present invention relates to a kind of plant extracts for preventing diabetes, and more specifically more particularly to one kind is used for
Prevent the vegetables and fruits extraction composition formula and preparation method of type II diabetes.
Background technology
Diabetes are a kind of characterized by hyperglycemia, the metabolic disease group of chronic, multifactor induction, be it is a kind of with
Chronic blood glucose levels increase the metabolic disease group being characterized.Long-standing hyperglycemia when diabetes, can cause multisystem
Damage, especially eye, kidney, heart, blood vessel, the chronic lesion of nerve, dysfunction, if diabetes do not obtain enough controls
System, can also cause some complication, serious complication to include：Angiocardiopathy, kidney failure, ablepsia, neuropathy, diabetes
Foot (or even amputation) etc., the incidence of diabetes are in continue ascendant trend in the world, and the death rate is only second to heart and brain blood
Pipe disease and cancer are referred to as the third-largest killer of human health.
According to hygiene department's statistical data in 2017, the current Type I diabetes patient in China was close to 6 million peoples, type II diabetes
For patient up to 8 million peoples, also more than 100,000,000 diabetes forerunner group, country need to be that Type I diabetes patient with severe symptoms consumption is huge every year
E Te cures subsidy fund, and diabetes bring heavy burden to social economy and personal lifestyle, and active prevention and treatment diabetes are
It is extremely urgent.
Patients with NIDDM is treated by oral drugs at present, because the characteristic of long-term administration, chemicals must
Certain toxic side effect can be so brought to patient, or even the initiation " drug induced disease " having；Chemicals can only take stopgap measures, and control
Liver kidney is further injured again while blood glucose, II type patient is accelerated and marches toward the paces of complication.
" integration of drinking and medicinal herbs " is one of the contribution to mankind's most worthy among Chinese original medicine, and Chinese medicine always emphasizes " medicine
Treat and be not so good as dietotherapy ", for chemicals are used for a long time there are the present situation of certain toxic side effect, people, which have invested sight, to be had
In the Chinese medicine or food materials of function of reducing blood sugar, public usually boiled medicine by technology restriction stews soup, stewes and fry or eat it raw, and all there is hypoglycemic work(
Imitate faint and with the worries of other bio-toxicities, traditional Chinese medicine high temperature decocts the Chinese medicine or health products of formula of stewing soup, each middle drug
Or the bioactivity degree of nutrition by high temperature totally, it is difficult to play effect, this be also clinical treatment diabetes almost by
The basic reason of Western medicine monopolization, and take toxic side effect caused by chemicals for a long time, damage diabetic liver, kidney
Equal tissues, make patients with NIDDM induce the increased risk of complication, for this purpose, it is proposed that a kind of for preventing II type sugar
The vegetables and fruits extraction composition formula and preparation method of urine disease.
Invention content
The purpose of the present invention is to solve disadvantages existing in the prior art, and the one kind proposed is for preventing II type sugar
The vegetables and fruits extraction composition formula and preparation method of urine disease, provide it is a kind of apply in chronic metabolism of blood glucose disease areas, by pure day
Right vegetables and fruits plant extracts is composed, is without any side effects, being added without external addition anti-corrosion, pigment chemical reagent, is used for
Prevent the vegetables and fruits plant extracts combination formula of type II diabetes.
To achieve the above object, the present invention provides the following technical solutions：A kind of vegetables and fruits for preventing type II diabetes carry
Composition formula and preparation method are taken, the raw material components of following parts by weight are included：5~30 parts of longevity greens/mustard green, 4~20 parts of konjaku, 3
~15 parts of sea-buckthorn, 2~10 parts to day fruit, 2-10 parts of pueraria lobata and 2-10 parts of Siraitia grosvenorii.
Preferably, a kind of vegetables and fruits plant extracts preparation method for preventing type II diabetes, includes the following steps：
S1, by clean longevity greens/mustard green, konjaku, sea-buckthorn, pueraria lobata, Siraitia grosvenorii and decladding to day fruit, low temperature dries spare；
S2, longevity greens/mustard green is crushed, 80-100 mesh sieve is crossed, with 45% ethanol as solvent, solid-to-liquid ratio 1：10, fully stir
It is even, 12 hours are stood, is extracted 30 minutes at≤45 DEG C, separation obtains longevity greens/mustard green extract, the longevity greens/mustard green after filtrate recycling ethanol
Residue is remained to extract in next step and be used again；
S3, decladding is broken to day fruit powder, mistake 80-100 mesh sieve, with 50% ethanol as solvent, solid-to-liquid ratio 1：10, fully
Stirring stands 12 hours, extract 30 minutes at≤45 DEG C, detaches and obtains to day berry extract, after filtrate recycling ethanol to day
Fruit residue is remained to extract in next step and be used again；
S4, it is mixed to day fruit residue and longevity greens/mustard green residue, with 90% ethanol as solvent, solid-to-liquid ratio 1：15, it fills
Point stirring, extract 50 minutes at≤45 DEG C, filters ethyl alcohol, then with extracting n-butyl alcohol, obtain butanol extraction liquid, by extracting n-butyl alcohol
Liquid is concentrated into appropriate washing with reduction vaporization method and discards, then is eluted with 30%~100% ethanol solution, the extraction that eluent obtains
Object is detached through HPLC again, and separation obtains alkaloids；
S5, sea-buckthorn is crushed, 80-100 mesh sieve is crossed, with 60% ethanol as solvent, solid-to-liquid ratio 1：15, it is sufficiently stirred,
12 hours are stood, is extracted 30 minutes at≤45 DEG C, filtrate recycling ethanol obtains hippophae rhamnoides；
S6, pueraria lobata is crushed, 100-120 mesh sieve is crossed, with water as solvent, solid-to-liquid ratio 1：20, be sufficiently stirred, 45 DEG C with
Under, vacuum degree be 0.1mmHg-1.0mmHg negative pressure extract 90 minutes, aqueous extract with macroreticular resin remove impurity, be concentrated under reduced pressure
Afterwards, Puerarin class extract is obtained；
S7, Siraitia grosvenorii is crushed, 80-100 mesh sieve is crossed, with water as solvent, solid-to-liquid ratio 1：15, it is sufficiently stirred, at 45 DEG C
Below, vacuum degree is that 0.1mmHg-1.0mmHg negative pressure is extracted 60 minutes, and aqueous extract macroreticular resin removes impurity, and decompression is dense
After contracting, Fructus Monordicae extract is obtained；
S8, konjaku is crushed, 80-100 mesh sieve is crossed, with 40% ethanol as solvent, solid-to-liquid ratio 1：10, it is sufficiently stirred,
12 hours are stood, is extracted 30 minutes at≤45 DEG C, filtrate recycling ethanol obtains glucomannan substance；
S9, above-mentioned all substances are mixed in a certain ratio, are sent into vacuum decompression drying machine, vacuum degree 0.1mmHg-
1.0mmHg, the dry obtained extract powder at≤45 DEG C.
The technique effect and advantage of the present invention：
1, than existing diabetes medicament, present invention harm without any side effects：Existing diabetes medicament is nearly all chemistry
Medicine, side effect endanger greatly patient, and inventive formulation composition derives from safe and non-toxic vegetables and fruits food materials.
2, the present invention is not simply milled primary raw material to match and stir evenly so simply, the required master of the present invention
It wants substance to be extracted item by item by advanced technology, rejects toxicant, leave behind useful composition, then carry out science again and match
Fang Ercheng.
3, the present invention jumps out traditional Chinese medicine must decoct the theory stewed soup through high temperature, use low temperature to extract skill in preparation process
Art, the activity degree damage of various nutriments make the big optimization of biological utilisation.
4, scientific formulation：By testing verification repeatedly, just reaches optimum formula, be full of intelligence and wisdom and the examination of research staff
Painstaking sweat during wrong.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, below in conjunction with specific embodiment, to this
Invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, not
For limiting the present invention.Based on the embodiments of the present invention, those of ordinary skill in the art are not before making creative work
The every other embodiment obtained is put, shall fall within the protection scope of the present invention.
Embodiment 1
A kind of vegetables and fruits plant extracts preparation method for preventing type II diabetes is made pulvis and is included the following steps：
S1,50 kilograms of longevity greens/mustard green, 40 kilograms of konjaku, 30 kilograms of sea-buckthorn, 20 kilograms of pueraria lobata, 20 kilograms of Siraitia grosvenorii are cleaned,
To the 20 kilograms of decladdings of day fruit, low temperature dries spare；
S2, longevity greens/mustard green is crushed, 80-100 mesh sieve is crossed, with 45% ethanol as solvent, solid-to-liquid ratio 1：10, fully stir
It is even, 12 hours are stood, is extracted 30 minutes at≤45 DEG C, separation obtains about 5 kilograms of longevity greens/mustard green extract, after filtrate recycling ethanol
Longevity greens/mustard green residue is remained to extract in next step and be used again；
S3, decladding is broken to day fruit powder, mistake 80-100 mesh sieve, with 50% ethanol as solvent, solid-to-liquid ratio 1：10, fully
Stirring stands 12 hours, is extracted 30 minutes at≤45 DEG C, and separation is obtained to about 3 kilograms of day berry extract, after filtrate recycling ethanol
To day fruit residue, remain to extract in next step and use again；
S4, it is mixed to day fruit residue and longevity greens/mustard green residue, with 90% ethanol as solvent, solid-to-liquid ratio 1：15, it fills
Point stirring, extract 50 minutes at≤45 DEG C, filters ethyl alcohol, then with extracting n-butyl alcohol, obtain butanol extraction liquid, by extracting n-butyl alcohol
Liquid is concentrated into appropriate washing with reduction vaporization method and discards, then is eluted with 30%~100% ethanol solution, the extraction that eluent obtains
Object is detached through HPLC again, and separation obtains about 2 kilograms of alkaloids；
S5, sea-buckthorn is crushed, 80-100 mesh sieve is crossed, with 60% ethanol as solvent, solid-to-liquid ratio 1：15, it is sufficiently stirred,
12 hours are stood, is extracted 30 minutes at≤45 DEG C, filtrate recycling ethanol obtains about 4 kilograms of hippophae rhamnoides；
S6, pueraria lobata is crushed, 100-120 mesh sieve is crossed, with water as solvent, solid-to-liquid ratio 1：20, be sufficiently stirred, 45 DEG C with
Under, vacuum degree be 0.1mmHg-1.0mmHg negative pressure extract 90 minutes, aqueous extract with macroreticular resin remove impurity, be concentrated under reduced pressure
Afterwards, about 3 kilograms of Puerarin class extract is obtained；
S7, Siraitia grosvenorii is crushed, 80-100 mesh sieve is crossed, with water as solvent, solid-to-liquid ratio 1：15, it is sufficiently stirred, at 45 DEG C
Below, vacuum degree is that 0.1mmHg-1.0mmHg negative pressure is extracted 60 minutes, and aqueous extract macroreticular resin removes impurity, and decompression is dense
After contracting, about 3 kilograms of Fructus Monordicae extract is obtained；
S8, konjaku is crushed, 80-100 mesh sieve is crossed, with 40% ethanol as solvent, solid-to-liquid ratio 1：10, it is sufficiently stirred,
12 hours are stood, is extracted 30 minutes at≤45 DEG C, filtrate recycling ethanol obtains about 6 kilograms of glucomannan substance；
S9, by the mixing of about 26 kilograms of above-mentioned all substances, be sent into vacuum decompression drying machine, vacuum degree 0.1mmHg-
1.0mmHg, dry obtained about 10 kilograms of extract powder, packed products at≤45 DEG C.
Embodiment 2
A kind of vegetables and fruits plant extracts preparation method for preventing type II diabetes, it includes following step to make granule
Suddenly：
S1,50 kilograms of longevity greens/mustard green, 40 kilograms of konjaku, 30 kilograms of sea-buckthorn, 20 kilograms of pueraria lobata, 20 kilograms of Siraitia grosvenorii are cleaned,
To the 20 kilograms of decladdings of day fruit, low temperature dries spare；
S2, longevity greens/mustard green is crushed, 80-100 mesh sieve is crossed, with 45% ethanol as solvent, solid-to-liquid ratio 1：10, fully stir
It is even, 12 hours are stood, is extracted 30 minutes at≤45 DEG C, separation obtains about 5 kilograms of longevity greens/mustard green extract, after filtrate recycling ethanol
Longevity greens/mustard green residue is remained to extract in next step and be used again；
S3, decladding is broken to day fruit powder, mistake 80-100 mesh sieve, with 50% ethanol as solvent, solid-to-liquid ratio 1：10, fully
Stirring stands 12 hours, is extracted 30 minutes at≤45 DEG C, and separation is obtained to about 3 kilograms of day berry extract, after filtrate recycling ethanol
To day fruit residue, remain to extract in next step and use again；
S4, it is mixed to day fruit residue and longevity greens/mustard green residue, with 90% ethanol as solvent, solid-to-liquid ratio 1：15, it fills
Point stirring, extract 50 minutes at≤45 DEG C, filters ethyl alcohol, then with extracting n-butyl alcohol, obtain butanol extraction liquid, by extracting n-butyl alcohol
Liquid is concentrated into appropriate washing with reduction vaporization method and discards, then is eluted with 30%~100% ethanol solution, the extraction that eluent obtains
Object is detached through HPLC again, and separation obtains about 2 kilograms of alkaloids；
S5, sea-buckthorn is crushed, 80-100 mesh sieve is crossed, with 60% ethanol as solvent, solid-to-liquid ratio 1：15, it is sufficiently stirred,
12 hours are stood, is extracted 30 minutes at≤45 DEG C, filtrate recycling ethanol obtains about 4 kilograms of hippophae rhamnoides；
S6, pueraria lobata is crushed, 100-120 mesh sieve is crossed, with water as solvent, solid-to-liquid ratio 1：20, be sufficiently stirred, 45 DEG C with
Under, vacuum degree be 0.1mmHg-1.0mmHg negative pressure extract 90 minutes, aqueous extract with macroreticular resin remove impurity, be concentrated under reduced pressure
Afterwards, about 3 kilograms of Puerarin class extract is obtained；
S7, Siraitia grosvenorii is crushed, 80-100 mesh sieve is crossed, with water as solvent, solid-to-liquid ratio 1：15, it is sufficiently stirred, at 45 DEG C
Below, vacuum degree is that 0.1mmHg-1.0mmHg negative pressure is extracted 60 minutes, and aqueous extract macroreticular resin removes impurity, and decompression is dense
After contracting, about 3 kilograms of Fructus Monordicae extract is obtained；
S8, konjaku is crushed, 80-100 mesh sieve is crossed, with 40% ethanol as solvent, solid-to-liquid ratio 1：10, it is sufficiently stirred,
12 hours are stood, is extracted 30 minutes at≤45 DEG C, filtrate recycling ethanol obtains about 6 kilograms of glucomannan substance；
S9, by the mixing of about 26 kilograms of above-mentioned all substances, 1 kilogram of denaturation cornstarch is added and does auxiliary material, it is full and uniform, lead to
Granulator granulation is crossed, vacuum drier is sent into after granulation, granule is made about after dry in vacuum degree 0.1mmHg-1.0mmHg
11 kilograms, packed products.
Embodiment 3
A kind of vegetables and fruits plant extracts preparation method for preventing type II diabetes is done piece agent and is included the following steps：
S1,50 kilograms of longevity greens/mustard green, 40 kilograms of konjaku, 30 kilograms of sea-buckthorn, 20 kilograms of pueraria lobata, 20 kilograms of Siraitia grosvenorii are cleaned,
To the 20 kilograms of decladdings of day fruit, low temperature dries spare；
S2, longevity greens/mustard green is crushed, 80-100 mesh sieve is crossed, with 45% ethanol as solvent, solid-to-liquid ratio 1：10, fully stir
It is even, 12 hours are stood, is extracted 30 minutes at≤45 DEG C, separation obtains about 5 kilograms of longevity greens/mustard green extract, after filtrate recycling ethanol
Longevity greens/mustard green residue is remained to extract in next step and be used again；
S3, decladding is broken to day fruit powder, mistake 80-100 mesh sieve, with 50% ethanol as solvent, solid-to-liquid ratio 1：10, fully
Stirring stands 12 hours, is extracted 30 minutes at≤45 DEG C, and separation is obtained to about 3 kilograms of day berry extract, after filtrate recycling ethanol
To day fruit residue, remain to extract in next step and use again；
S4, it is mixed to day fruit residue and longevity greens/mustard green residue, with 90% ethanol as solvent, solid-to-liquid ratio 1：15, it fills
Point stirring, extract 50 minutes at≤45 DEG C, filters ethyl alcohol, then with extracting n-butyl alcohol, obtain butanol extraction liquid, by extracting n-butyl alcohol
Liquid is concentrated into appropriate washing with reduction vaporization method and discards, then is eluted with 30%~100% ethanol solution, the extraction that eluent obtains
Object is detached through HPLC again, and separation obtains about 2 kilograms of alkaloids；
S5, sea-buckthorn is crushed, 80-100 mesh sieve is crossed, with 60% ethanol as solvent, solid-to-liquid ratio 1：15, it is sufficiently stirred,
12 hours are stood, is extracted 30 minutes at≤45 DEG C, filtrate recycling ethanol obtains about 4 kilograms of hippophae rhamnoides；
S6, pueraria lobata is crushed, 100-120 mesh sieve is crossed, with water as solvent, solid-to-liquid ratio 1：20, be sufficiently stirred, 45 DEG C with
Under, vacuum degree be 0.1mmHg-1.0mmHg negative pressure extract 90 minutes, aqueous extract with macroreticular resin remove impurity, be concentrated under reduced pressure
Afterwards, about 3 kilograms of Puerarin class extract is obtained；
S7, Siraitia grosvenorii is crushed, 80-100 mesh sieve is crossed, with water as solvent, solid-to-liquid ratio 1：15, it is sufficiently stirred, at 45 DEG C
Below, vacuum degree is that 0.1mmHg-1.0mmHg negative pressure is extracted 60 minutes, and aqueous extract macroreticular resin removes impurity, and decompression is dense
After contracting, about 3 kilograms of Fructus Monordicae extract is obtained；
S8, konjaku is crushed, 80-100 mesh sieve is crossed, with 40% ethanol as solvent, solid-to-liquid ratio 1：10, it is sufficiently stirred,
12 hours are stood, is extracted 30 minutes at≤45 DEG C, filtrate recycling ethanol obtains about 6 kilograms of glucomannan substance；
S9, by the mixing of about 26 kilograms of above-mentioned all substances, be sent into vacuum decompression drying machine, vacuum degree 0.1mmHg-
1.0mmHg, dry obtained about 10 kilograms of the extract powder at≤45 DEG C；
1 kilogram of S10, addition denaturation cornstarch do auxiliary material, full and uniform, and by tabletting machine, it is public to obtain tablet about 11
Jin, packed products.
Embodiment 4
A kind of vegetables and fruits plant extracts preparation method for preventing type II diabetes, it includes following step to make capsule
Suddenly：
S1,50 kilograms of longevity greens/mustard green, 40 kilograms of konjaku, 30 kilograms of sea-buckthorn, 20 kilograms of pueraria lobata, 20 kilograms of Siraitia grosvenorii are cleaned,
To the 20 kilograms of decladdings of day fruit, low temperature dries spare；
S2, longevity greens/mustard green is crushed, 80-100 mesh sieve is crossed, with 45% ethanol as solvent, solid-to-liquid ratio 1：10, fully stir
It is even, 12 hours are stood, is extracted 30 minutes at≤45 DEG C, separation obtains about 5 kilograms of longevity greens/mustard green extract, after filtrate recycling ethanol
Longevity greens/mustard green residue is remained to extract in next step and be used again；
S3, decladding is broken to day fruit powder, mistake 80-100 mesh sieve, with 50% ethanol as solvent, solid-to-liquid ratio 1：10, fully
Stirring stands 12 hours, is extracted 30 minutes at≤45 DEG C, and separation is obtained to about 3 kilograms of day berry extract, after filtrate recycling ethanol
To day fruit residue, remain to extract in next step and use again；
S4, it is mixed to day fruit residue and longevity greens/mustard green residue, with 90% ethanol as solvent, solid-to-liquid ratio 1：15, it fills
Point stirring, extract 50 minutes at≤45 DEG C, filters ethyl alcohol, then with extracting n-butyl alcohol, obtain butanol extraction liquid, by extracting n-butyl alcohol
Liquid is concentrated into appropriate washing with reduction vaporization method and discards, then is eluted with 30%~100% ethanol solution, the extraction that eluent obtains
Object is detached through HPLC again, and separation obtains about 2 kilograms of alkaloids；
S5, sea-buckthorn is crushed, 80-100 mesh sieve is crossed, with 60% ethanol as solvent, solid-to-liquid ratio 1：15, it is sufficiently stirred,
12 hours are stood, is extracted 30 minutes at≤45 DEG C, filtrate recycling ethanol obtains about 4 kilograms of hippophae rhamnoides；
S6, pueraria lobata is crushed, 100-120 mesh sieve is crossed, with water as solvent, solid-to-liquid ratio 1：20, be sufficiently stirred, 45 DEG C with
Under, vacuum degree be 0.1mmHg-1.0mmHg negative pressure extract 90 minutes, aqueous extract with macroreticular resin remove impurity, be concentrated under reduced pressure
Afterwards, about 3 kilograms of Puerarin class extract is obtained；
S7, Siraitia grosvenorii is crushed, 80-100 mesh sieve is crossed, with water as solvent, solid-to-liquid ratio 1：15, it is sufficiently stirred, at 45 DEG C
Below, vacuum degree is that 0.1mmHg-1.0mmHg negative pressure is extracted 60 minutes, and aqueous extract macroreticular resin removes impurity, and decompression is dense
After contracting, about 3 kilograms of Fructus Monordicae extract is obtained；
S8, konjaku is crushed, 80-100 mesh sieve is crossed, with 40% ethanol as solvent, solid-to-liquid ratio 1：10, it is sufficiently stirred,
12 hours are stood, is extracted 30 minutes at≤45 DEG C, filtrate recycling ethanol obtains about 6 kilograms of glucomannan substance；
S9, by the mixing of about 26 kilograms of above-mentioned all substances, be sent into vacuum decompression drying machine, vacuum degree 0.1mmHg-
1.0mmHg, dry obtained about 10 kilograms of the extract powder at≤45 DEG C；
S10, by about 10 kilograms of extract powder, be sent into capsule filling machine, hard capsule or soft capsule packed products be made.
Test example 1
(1) test material：Extract powder prepared by the embodiment of the present invention 1.
(2) subjects：II diabetic 48, wherein male 26, women 22, the course of disease 1~3 year.
Patient is divided into two groups, every group of each 24 patient, control group is 12 male, female 12, average age (55.83 ±
2.85) year.Treatment group male 14, women 10, average age (58.94 ± 2.95) year.All patients are diabetes B
Patient and with hyperlipemia.48 patients are before reception test, all without use of exogenous insulin.Two groups of patients height,
Weight, gender, age etc. no significant difference (P>0.05).
(3) therapy：Control group:Its blood fat is detected with blood glucose before treatment, and is suffered from by diabetic diet management
Person's diet, low fat, less salt.In addition to this, also to remind patient that can carry out moderate exercise after a meal；Treatment group:It is right before treatment
Its blood fat is detected with blood glucose, and presses diabetic diet management, and carries out modest movement, provides mouth on this basis for patient
The vegetables and fruits plant extract powders treatment of the clothes present invention, respectively takes 8g before empty stomach sooner or later, persistently takes 30 days
(4) treatment effectiveness evaluation standard：Significant effect:Blood fat declines apparent with blood glucose.Effectively:Under blood fat, blood glucose have
Drop, fall are little.In vain：Blood fat, blood glucose and treatment are preceding without significant change.Total effective rate=(significant effect number of cases+effectively
Number of cases)/total number of cases × 100%.
(5) observation index：It is each at the 7th day, the 15th day and the 30th day respectively to the fasting blood-glucose of two groups of patients, postprandial blood sugar
Detection is primary, totally 3 times；At the 15th day and the 30th day, each detection was primary respectively for glycosylated hemoglobin, triglyceride, totally 2 times；Observation
Fasting blood-glucose, the specific situation of change of postprandial blood sugar, glycosylated hemoglobin, triglyceride of the patient between 30d.
(6) statistical method：The analysis of data, measurement data are carried out to above-mentioned treatment using SPSS16.0 statistics softwares
It is indicated using mean ± standard deviation (x ± s), carry out t inspections, enumeration data carries out chi-square criterion, has system for difference when P ＜ 0.05
Meter learns meaning.
(7) result：
1 two groups of pretherapy and post-treatment fasting blood glucose levels of patient of table compare
1 two groups of pretherapy and post-treatment fasting blood glucose levels of patient of table compare [FBG (mmol/L)]
From table 2 it can be seen that the equal no significant difference of fasting blood glucose level (* P ＞ 0.05) before two groups of patient's treatments, having can
Compare property.After treatment 30 days, treatment group's fasting blood-glucose is significantly lower than control group, and ▲ P ＜ 0.05 have statistical significance.
2 two groups of pretherapy and post-treatment level of postprandial blood sugar of patient of table compare
2 two groups of pretherapy and post-treatment level of postprandial blood sugar of patient of table compare [2h PBG (mmol/L)]
From table 2 it can be seen that the equal no significant difference of postprandial 2 hours blood glucose levels (* P ＞ 0.05) before two groups of patient's treatments,
It is comparable.After treatment 30 days, the postprandial 2 hours blood glucose for the treatment of group is significantly lower than control group, and ▲ P ＜ 0.05 have statistics
Meaning.
3 two groups of pretherapy and post-treatment glycated hemoglobin levels of patient of table compare
3 two groups of pretherapy and post-treatment glycated hemoglobin levels of patient of table compare [HbA1c (mmol/L]
From table 3 it can be seen that the equal no significant difference of glycated hemoglobin levels (* P ＞ 0.05) before two groups of patient's treatments, tool
There is comparativity.It after treatment 30 days, is reduced before glycosylated hemoglobin is relatively treated after treatment group 30 days, Δ P ＜ 0.05 have statistics
Meaning, glycosylated hemoglobin is less than control group after treatment group 30 days, and ▲ P ＜ 0.05 have statistical significance.
4 two groups of pretherapy and post-treatment triglyceride levels of patient of table compare
4 two groups of pretherapy and post-treatment triglyceride levels of patient of table compare [TG (mmol/L)]
From table 4, it can be seen that the equal no significant difference of triglyceride levels (* P ＞ 0.05) before two groups of patient's treatments, having can
Compare property.After treatment 30 days, treatment group's triglycerides is significantly lower than control group, and ▲ P ＜ 0.05 have statistical significance.
The pretherapy and post-treatment indices effect of 5 two groups of patients of table compares
The pretherapy and post-treatment indices effect of 5 two groups of patients of table compares [↓ (%)]
As can be seen from Table 5, diabetic diet managing patient diet, low fat, less salt and appropriate fortune are pressed to two groups of patients
After dynamic management, the blood glucose and triglycerides index of control group are declined after 30 days；Treatment group is in above-mentioned diet and motion pipe
Under the premise of reason, the vegetables and fruits plant extract powders of the present invention are taken orally after 30 days, and indices significantly reduce, and treatment group patient takes
Obtained preferable therapeutic effect.
To sum up, diabetic science diet and moderate exercise on the basis of, take orally the present invention vegetables and fruits plant carry
Object combination formula is taken, can effectively reduce blood glucose and blood fat, and the raw material of the present invention is derived from vegetables and fruits food, it can be long-term
It takes, there is positive effect for prevention type II diabetes.
In summary：A kind of vegetables and fruits extraction composition formula and preparation side for preventing type II diabetes provided by the invention
Method, compared with prior art, provide it is a kind of apply in chronic metabolism of blood glucose disease areas, by pure natural vegetables and fruits plant extract
Object is composed, is without any side effects, being added without external addition anti-corrosion, pigment chemical reagent, for preventing type II diabetes
Vegetables and fruits plant extracts combination formula.
Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
With technical scheme described in the above embodiments is modified or equivalent replacement of some of the technical features,
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in the present invention's
Within protection domain.
Claims (2)
1. a kind of vegetables and fruits extraction composition formula and preparation method for preventing type II diabetes, it is characterised in that：Including following heavy
Measure the raw material components of number：5~30 parts of longevity greens/mustard green, 4~20 parts of konjaku, 3~15 parts of sea-buckthorn, 2~10 parts to day fruit,
The Siraitia grosvenorii of 2-10 parts of pueraria lobata and 2-10 part.
2. a kind of vegetables and fruits plant extracts preparation method for preventing type II diabetes according to claim 1, special
Sign is：Include the following steps：
S1, by clean longevity greens/mustard green, konjaku, sea-buckthorn, pueraria lobata, Siraitia grosvenorii and decladding to day fruit, low temperature dries spare；
S2, longevity greens/mustard green is crushed, 80-100 mesh sieve is crossed, with 45% ethanol as solvent, solid-to-liquid ratio 1：10, fully stir evenly, it is quiet
It sets 12 hours, is extracted 30 minutes at≤45 DEG C, separation obtains longevity greens/mustard green extract, and the longevity greens/mustard green after filtrate recycling ethanol is remaining
Object is remained to extract in next step and be used again；
S3, decladding is broken to day fruit powder, mistake 80-100 mesh sieve, with 50% ethanol as solvent, solid-to-liquid ratio 1：10, fully stir
Mix, stand 12 hours, extract 30 minutes at≤45 DEG C, detach and obtain to day berry extract, after filtrate recycling ethanol to day fruit
Residue is remained to extract in next step and be used again；
S4, it is mixed to day fruit residue and longevity greens/mustard green residue, with 90% ethanol as solvent, solid-to-liquid ratio 1：15, fully stir
Mix, extracted 50 minutes at≤45 DEG C, filter ethyl alcohol, then with extracting n-butyl alcohol, butanol extraction liquid, butanol extraction liquid is used
Reduction vaporization method is concentrated into appropriate washing and discards, then is eluted with 30%~100% ethanol solution, and the extract that eluent obtains is again
It is detached through HPLC, separation obtains alkaloids；
S5, sea-buckthorn is crushed, 80-100 mesh sieve is crossed, with 60% ethanol as solvent, solid-to-liquid ratio 1：15, it is sufficiently stirred, stands
It 12 hours, is extracted 30 minutes at≤45 DEG C, filtrate recycling ethanol obtains hippophae rhamnoides；
S6, pueraria lobata is crushed, 100-120 mesh sieve is crossed, with water as solvent, solid-to-liquid ratio 1：20, be sufficiently stirred, 45 DEG C or less,
Vacuum degree is that 0.1mmHg-1.0mmHg negative pressure is extracted 90 minutes, and aqueous extract removes impurity with macroreticular resin, after reduced pressure,
Obtain Puerarin class extract；
S7, Siraitia grosvenorii is crushed, 80-100 mesh sieve is crossed, with water as solvent, solid-to-liquid ratio 1：15, be sufficiently stirred, 45 DEG C or less,
Vacuum degree is that 0.1mmHg-1.0mmHg negative pressure is extracted 60 minutes, and aqueous extract removes impurity with macroreticular resin, after reduced pressure,
Obtain Fructus Monordicae extract；
S8, konjaku is crushed, 80-100 mesh sieve is crossed, with 40% ethanol as solvent, solid-to-liquid ratio 1：10, it is sufficiently stirred, stands
It 12 hours, is extracted 30 minutes at≤45 DEG C, filtrate recycling ethanol obtains glucomannan substance；
S9, above-mentioned all substances are mixed in a certain ratio, are sent into vacuum decompression drying machine, vacuum degree 0.1mmHg-
1.0mmHg, the dry obtained extract powder at≤45 DEG C.
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