CN110013497A - A kind of Chinese medicinal soft capsule preparation process that immunity can be improved - Google Patents
A kind of Chinese medicinal soft capsule preparation process that immunity can be improved Download PDFInfo
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Abstract
The present invention relates to a kind of Chinese medicinal soft capsule preparation processes that immunity can be improved, and include the following steps: the preparation of ganoderma Lucidum spore essential oil；The preparation of Reishi sporule medicinal powder；The preparation of ginseng, wilsonii and Radix Astragali mixed powder；The preparation of Medicinal powder of Rhodiola rosea L.；The preparation of capsule material；The preparation of soft capsule.Soft capsule is prepared using lucidum spore powder, ginseng, wilsonii, Radix Astragali and root of kirilow rhodiola, has the function of improving immunity well to human body, and be prepared into soft capsule, can effectively avoid the Chinese medicine when taking, bitter taste, it is difficult to the problem of swallowing.The preparation process of the Chinese medicinal soft capsule can effectively extract the effective component in medicinal material, perform to the effect of medicinal material bigger；Four properties of medicinal herb theory prescription quantization principles are made full use of simultaneously, make mutually to act synergistically between various substances.Thus more preferably, and the production process period is short for the soft capsule health-care effect that process obtains, and equipment investment is few, and low energy consumption, is more suitable for industrialized production.
Description
Technical field
The present invention relates to a kind of Chinese medicinal soft capsule preparation processes that immunity can be improved, and are related to technical field of traditional Chinese medicines.
Background technique
With social development, competition be growing more intense, rhythm of life is constantly accelerated, and the pressure that people are born is increasingly
Greatly, the physical condition of people also declines therewith, passive protective physical fitness decline；The namely medically described immunity of resistance, it
It is a kind of ability that human immune system carries out self-protection, one of main effect refers to body resistance bacterium or virus etc.
The ability of infection.Immunity is the factor for having substantial connection with aging, and immunocyte decline is the most important reason of aging
One of.
With society and economic progress, the improvement of people's living standards, scientific and technical development, the change of disease pattern
And the pursuit to good health and a long life, the functional food of strengthen immunity gradually everybody deep heart, people have started from treating again
Steering prevents again.China possesses the huge treasure-house of this functional food of Chinese herbal medicine and other characteristic resources, we should be in conjunction with increasing
The functional food development trend of strong immunity and the impetus of China's development, make great efforts to excavate these precious resources, in fierceness
International competition in obtain due status.
Summary of the invention
The present invention is in view of the deficienciess of the prior art, provide a kind of preparation work of Chinese medicinal soft capsule that immunity can be improved
Skill, it is an advantage of the invention that the effective component extracting in Chinese medicinal plant, and the interaction between each Chinese medicinal plant is utilized,
To play the role of improving immunity to human body, and the step of preparation process is simple, can make full use of in Chinese medicinal plant
Effective component, low energy consumption, and equipment investment is few, is more suitable for industrialized production.
The technical scheme to solve the above technical problems is that a kind of Chinese medicinal soft capsule preparation that immunity can be improved
Technique includes the following steps:
(1) preparation of ganoderma Lucidum spore essential oil: in parts by weight, 800-1200 parts of progress titanium dioxides of lucidum spore powder are taken
Carbon supercritical fluid extraction, obtains ganoderma Lucidum spore essential oil；The Reishi sporule obtained using the method for carbon dioxide supercritical extraction
Essential oil is more clean, safe.
(2) preparation of Reishi sporule medicinal powder: in step (1), remaining Reishi sporule residue after ganoderma Lucidum spore essential oil is extracted
It is impregnated 1.5 hours, is then centrifuged for, then dilute hydrochloric acid is added in Reishi sporule residue under ultrasonic wave effect with dilute hydrochloric acid solution
Solution is centrifuged after impregnating 1.5 hours under ultrasonic wave acts on, and merges the solution after impregnating twice, filter, concentration, be dried in vacuo,
It crushes, obtains Reishi sporule medicinal powder.Immersion efficiency can be improved in the effect of ultrasonic wave, shortens soaking time, to make more
Effective component is dipped in solution；It is impregnated using two-step method, using dissolution equilibrium principle, can sufficiently leach Reishi sporule
Effective component in residue；It selects the mode of centrifugation to be separated by solid-liquid separation after immersion, separative efficiency can be improved, because
Reishi sporule residue is finer and smoother to be sticked together, and can be easy blocking filter cloth using conventional filtering, be caused separative efficiency low.
(3) preparation of ginseng, wilsonii and Radix Astragali mixed powder: in parts by weight, by 400-600 parts of ginseng, thorn
400-600 parts and Radix Astragali 800-1000 parts choppings of slender acanthopanax, are added ethyl alcohol progress first time heating and refluxing extraction 1.5 hours, then
After the solution of first time refluxing extraction is filtered out, adds ethyl alcohol and carry out second heating and refluxing extraction 1.5 hours, merge two
The solution of secondary refluxing extraction, filtering, vacuum drying, crush at concentration, obtain ginseng, wilsonii and Radix Astragali mixed powder.By people
It is mixed after ginseng, wilsonii and Radix Astragali chopping, then carries out refluxing extraction, phase interaction between ginseng, wilsonii and Radix Astragali can be made
With so that it is milder to act on the active principle extracted to human body；Using refluxing extraction is added twice, extraction efficiency is more
Height, and the usage amount of ethyl alcohol is reduced, reduce the energy consumption of later period concentration.
(4) preparation of Medicinal powder of Rhodiola rosea L.: in parts by weight, by 800-1000 parts of root of kirilow rhodiola shred, be added ethyl alcohol into
Row first time heating and refluxing extraction 1.5 hours after then filtering out the solution of first time refluxing extraction, adds ethyl alcohol progress
Second heating and refluxing extraction 1.5 hours, merge the solution of refluxing extraction twice, filtering, vacuum drying, crushes at concentration, obtains
To Medicinal powder of Rhodiola rosea L..Using refluxing extraction is added twice, extraction efficiency is higher, and reduces the usage amount of ethyl alcohol, reduces the later period
The energy consumption of concentration.
(5) preparation of capsule material: ganoderma Lucidum spore essential oil obtained in step (1) is heated to 40-50 DEG C, in stirring
In the case of sequentially add the Reishi sporule medicinal powder of step (2), the mixed powder of step (3), step (4) Medicinal powder of Rhodiola rosea L., stir
It mixes uniformly, adds medicinal plant oil, keep the temperature and stir evenly at 40-50 DEG C, cross colloid mill twice, sieve with 100 mesh sieve, then
High-pressure homogeneous processing is carried out, vacuumize process obtains capsule material.Crossing colloid mill and high-pressure homogeneous processing can make in capsule material
Material by effective emulsification, dispersion and crush, reach material fining Crushing of Ultrafine effect, thus more conducively absorption of human body；It takes out true
Vacancy reason can effectively remove the bubble in capsule material, be more favorable for the preparation of soft capsule, and easy to operate, equipment investment
It is few, it is more suitable for industrializing.
(6) soft capsule that content is 0.55g is made in capsule material, cooling, whole grain, washing, drying are to get in described
Medicine soft capsule.
Further, in parts by weight, 1000 parts of the lucidum spore powder, 500 parts of the ginseng, the Radix Astragali
1000 parts, it is 500 parts of the wilsonii, 1000 parts of the root of kirilow rhodiola, medicinal plant oil 200-300 parts described.The Reishi sporule
The phagocytic function of powder energy activating macrophage, has antagonism to glucocorticoid, can enhance non-specific immune function, mention
High humoral immunity level and cellular immune level, and there is apparent antagonism to hepatic injury caused by D-Gal,
With hypoglycemic, reducing blood lipid, the neurasthenic effect of adjustment.With panaxoside to the lipid-metabolism of intact animal in ginseng
There is facilitation, the biosynthesis of lipoprotein in cholesterol and blood, decomposition and inversion, excretion can be made to accelerate, can finally make gallbladder in blood
Sterol reduces, and has effect for reducing blood fat, can increase the enzymatic activity of each substance of liver metabolism, enhance the detoxification ability of liver, from
And enhancing body to the tolerance of various chemical substances, these are all conducive to improve immunity.Ginseng, which biases toward, to reinforce vital energy,
Radix Astragali is then based on qi-restoratives, both Chinese medicines, which merge to use, has extraordinary effect, and Radix Astragali has enhancing immunity of organism
Function, liver protection, diuresis, anti-aging, resisting stress, decompression and wide antibacterial action.Wilsonii has bowl spares strengthening the essence, hard muscle
The effect of bone, Qiang Zhiyi has similar pharmacological action with ginseng, but more mild.Root of kirilow rhodiola has cardiac effect, resists
Fatigue effect, Hardy dwarfing.Physical function, strengthen immunity can be enhanced in the above several drugs collaboration.
Further, the medicinal plant oil is one in sesame oil, olive oil, sunflower oil, corn oil and grape seed oil
Kind or a variety of mixing.One of sesame oil, olive oil, sunflower oil, corn oil and grape seed oil or a variety of mixing are as medicinal
Vegetable oil, for dispersing effective component, and these types of vegetable oil all has antioxidation, not only can be in softening glue
Play the role of peptizaiton in capsule, also acts as collaboration and improve immunity.
Further, in step (1), the extraction temperature of the co_2 supercritical fluid extraction is 40-50 DEG C, CO2
Fluid pressure is 15-25Mpa, CO2Flow is 15-25L/h, extraction time 60-90min.In the CO 2 supercritical stream
Under body extraction conditions, extraction efficiency is higher.
Further, it in step (2), impregnates every time, the weight ratio of Reishi sporule residue and dilute hydrochloric acid is 1:5, described dilute
The pH of hydrochloric acid is 5-6, and the soaking temperature of the dilute hydrochloric acid is 40-50 DEG C.PH is used to be impregnated for the dilute hydrochloric acid of 5-6, it can be with
Effective component in high efficiency extraction Reishi sporule residue, and acidity is suitable for, and obtained product will not generate stimulation to people's stomach,
And under the conditions of the pH, harmless to equipment, equipment investment is few in production process.
Further, in step (3), the ethyl alcohol is the ethyl alcohol of content 70%, the chopping of when first time refluxing extraction
The weight ratio of rear ginseng, the total weight of wilsonii and Radix Astragali and 70% ethyl alcohol is 1:4, when second of refluxing extraction described in cut
The total weight of ginseng, wilsonii and Radix Astragali after broken and the weight ratio of 70% ethyl alcohol are 1:5；Because of ginseng, wilsonii and Radix Astragali
In effective component, the existing substance for being dissolved in water also had the substance for being dissolved in alcohol, can efficiently be mentioned using the ethyl alcohol of content 70%
Active principle is taken, the improper effective component that will lead to extraction of the content of ethyl alcohol is relatively simple, leads to the soft capsule effect of preparation
It is poor.
Further, in step (4), the ethyl alcohol is the ethyl alcohol of content 90%, the chopping of when first time refluxing extraction
Rear root of kirilow rhodiola weight and 90% ethyl alcohol weight ratio are 1:4, the root of kirilow rhodiola weight after the chopping of when second of refluxing extraction with
90% ethyl alcohol weight ratio is 1:5.Use the ethyl alcohol of content 90% can be with the effective component in high efficiency extraction root of kirilow rhodiola.
Further, in step (2), step (3) and step (4), the concentration process is using vacuum distillation, concentration temperature
Degree is 40-60 DEG C, and concentration pressure is 10-50Kpa；The vacuum drying temperature is 40-60 DEG C.It can be mentioned using vacuum distillation
Highly enriched efficiency, and avoid thickening temperature excessively high, cause effective component to be denaturalized, and influence the effect of soft capsule；Using vacuum
Dry, rate of drying is fast, and drying temperature is low, it is ensured that effective component invariance, and also low energy consumption, the time is short, is more suitable for industrializing
Production.
Further, in step (5), pressure condition when described high-pressure homogeneous is 5-20Mpa, the vacuumize process
Temperature be 0-5 DEG C, the pressure of the vacuumize process is 1-5Kpa.The condition of vacuumize process both may insure that bubble was gone
It removes, and ganoderma Lucidum spore essential oil and medical herb oil can be prevented by volatilization loss.
The beneficial effects of the present invention are:
(1) soft capsule is prepared using lucidum spore powder, ginseng, wilsonii, Radix Astragali and root of kirilow rhodiola, there is drop blood to human body
The effects of sugar, reducing blood lipid, adjustment neurasthenia, resisting fatigue, liver protection, diuresis, anti-aging, has and improve immunity well
Effect, and be prepared into soft capsule, can effectively avoid the Chinese medicine when taking, bitter taste, it is difficult to the problem of swallowing.
(2) it is not only only capable of using sesame oil, olive oil, sunflower oil, corn oil and grape seed oil as medicinal plant oil
It is enough to play the role of peptizaiton in softening capsule, it also acts as collaboration and improves immunity.
(3) in the preparation process, Reishi sporule medicinal powder is extracted using dilute hydrochloric acid, and Medicinal powder of Rhodiola rosea L. uses 90% ethyl alcohol
It extracts, ginseng, wilsonii and Radix Astragali mixed powder are extracted using 70% ethyl alcohol, in this way, by Reishi sporule medicinal powder
It is extracted respectively with Medicinal powder of Rhodiola rosea L., and uses different Extraction solvents, can effectively extract the effective component in medicinal material,
Perform to the effect of medicinal material bigger；It will extract, then can be made full use of after ginseng, wilsonii and Radix Astragali compounding simultaneously
Four properties of medicinal herb theory prescription quantization principles make mutually to act synergistically between various substances.Thus the soft capsule that process obtains
More preferably, and the production process period is short for health-care effect, and equipment investment is few.
(4) the Chinese medicinal soft capsule preparation process that immunity can be improved, whole technological process is high-efficient, the production cycle
Short, low energy consumption, and equipment investment is few, is more suitable for industrialized production.
Specific embodiment
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, below to specific reality of the invention
The mode of applying is described in detail.In the following description, numerous specific details are set forth in order to facilitate a full understanding of the present invention.But
The invention can be embodied in many other ways as described herein, and those skilled in the art can be without prejudice to originally
Similar improvement is done in the case where invention intension, therefore the present invention is not limited by the specific embodiments disclosed below.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to describe
The purpose of specific embodiment, it is not intended that in the limitation present invention.
Embodiment 1
A kind of Chinese medicinal soft capsule preparation process that immunity can be improved, includes the following steps:
(1) preparation of ganoderma Lucidum spore essential oil: in parts by weight, take 1000 parts of progress carbon dioxide of lucidum spore powder super
Supercritical fluid extraction, extraction temperature are 40-50 DEG C, CO2Fluid pressure is 20Mpa, CO2Flow is 15-25L/h, extracts 60min
Obtain ganoderma Lucidum spore essential oil；
(2) preparation of Reishi sporule medicinal powder: in step (1), remaining Reishi sporule residue after ganoderma Lucidum spore essential oil is extracted
In, 5000 parts of dilute hydrochloric acid solution of pH=6 are added, impregnates 1.5 hours, is then centrifuged for, then in ganoderma lucidum spore under ultrasonic wave effect
5000 parts of dilute hydrochloric acid solution of pH=6 are added in sub- residue, impregnates 1.5 hours, merges after impregnating twice under ultrasonic wave effect
Solution, filtering, filtrate be evaporated under reduced pressure concentration at 60 DEG C, and 60 DEG C are dried in vacuo, and crushing obtains Reishi sporule medicinal powder；
(3) preparation of ginseng, wilsonii and Radix Astragali mixed powder: in parts by weight, by 500 parts of ginseng, wilsonii
500 parts and 1000 parts of Radix Astragali choppings, the ethyl alcohol that 8000 parts of contents 70% are added carry out first time heating and refluxing extraction 1.5 hours,
Then after the solution of first time refluxing extraction being filtered out, the ethyl alcohol for adding 10000 parts of contents 70% carries out second and heats back
Stream extracts 1.5 hours, merges the solution of refluxing extraction twice, filtering, and filtrate is evaporated under reduced pressure concentration, 45 DEG C of vacuum at 45 DEG C
It dry, pulverize, obtain ginseng, wilsonii and Radix Astragali mixed powder；
(4) preparation of Medicinal powder of Rhodiola rosea L.: in parts by weight, 1000 parts of root of kirilow rhodiola is shredded, 4000 parts of contents are added
90% ethyl alcohol carries out first time heating and refluxing extraction 1.5 hours, after then the solution of first time refluxing extraction is filtered out, then
The ethyl alcohol that 5000 parts of contents 90% are added carries out second heating and refluxing extraction 1.5 hours, merges the molten of refluxing extraction twice
Liquid, filtering, filtrate are evaporated under reduced pressure concentration at 40 DEG C, and 40 DEG C of vacuum drying crush, obtain Medicinal powder of Rhodiola rosea L.；
(5) preparation of capsule material: being heated to 45 DEG C for ganoderma Lucidum spore essential oil obtained in step (1), the case where stirring
Under sequentially add the Reishi sporule medicinal powder of step (2), the mixed powder of step (3), step (4) Medicinal powder of Rhodiola rosea L., stirring is equal
It is even, 250 parts of olive oil are added, keeps the temperature and stirs evenly at 40-50 DEG C, colloid mill is crossed twice, sieves with 100 mesh sieve, then
High-pressure homogeneous processing is carried out under the conditions of 10Mpa, vacuumize process under the conditions of 0 DEG C obtains capsule material；
(6) content is made in capsule material is soft capsule 1000 of 0.55g, cooling, whole grain, washing, drying to get
The Chinese medicinal soft capsule, dose: 2 times a day, 2 tablets each time.
Embodiment 2
A kind of Chinese medicinal soft capsule preparation process, includes the following steps:
(1) preparation of ganoderma Lucidum spore essential oil: in parts by weight, take 1000 parts of progress carbon dioxide of lucidum spore powder super
Supercritical fluid extraction, extraction temperature are 40-50 DEG C, CO2Fluid pressure is 20Mpa, CO2Flow is 15-25L/h, extracts 60min
Obtain ganoderma Lucidum spore essential oil；
(2) preparation of mixed powder: remaining Reishi sporule residue adds after extracting ganoderma Lucidum spore essential oil in step (1)
Enter into extraction kettle, then 1000 parts of 500 parts of 500 parts of ginseng, wilsonii, the Radix Astragali and root of kirilow rhodiola of chopping are added in extracting kettle
1000 parts, and 70% 12000 parts of ethyl alcohol progress heating and refluxing extraction 2 hours is added, after filtering out solution, add 70%
Progress heating and refluxing extraction 2 hours of 15000 parts of ethyl alcohol, merge the solution twice after heating and refluxing extraction, filtering, filtrate exists
Concentration is evaporated under reduced pressure at 45 DEG C, 45 DEG C of vacuum drying crush, obtain mixed powder；
(3) preparation of capsule material: being heated to 45 DEG C for ganoderma Lucidum spore essential oil obtained in step (1), the case where stirring
Under sequentially add the mixed powders of step (2), stir evenly, add 250 parts of olive oil, keep the temperature and stir at 40-50 DEG C
Uniformly, it crosses colloid mill twice, sieves with 100 mesh sieve, then carry out high-pressure homogeneous processing under the conditions of 10Mpa, vacuumized under the conditions of 0 DEG C
Processing, obtains capsule material；
(6) soft capsule that content is 0.55g is made in capsule material, cooling, whole grain, washing, drying are to get in described
Medicine soft capsule.
The soft capsule that embodiment 1 and embodiment 2 are obtained tests the effect experiment of strengthen immunity:
1, sample and dosage: the soft capsule that Examples 1 and 2 are prepared, adult is every to take 2 times day by day, each taking
2, every content is 0.55g.It converts according to adult weight 60kg, test average mice body weight 20g, every
Mouse need to take 0.06% amount of soft capsule content, daily intake 2 times.
2, experimental animal is grouped, and each test experiments take 40 male mices, are divided into four groups: A group, B group, C group, D
There are 10 male mices, mouse weight 18-22g in group, every group of the inside.A group mouse is blank assay group, normal to raise；B group
Mouse is control group, and the olive oil of each 0.06% amount of feeding soft capsule content, daily feeding is twice；C group mouse, every time
The 0.06% of 1 soft capsule content of feeding embodiment, daily feeding is twice；D group mouse, in each 2 soft capsule of feeding embodiment
Tolerant 0.06%, daily feeding twice, are persistently fed 30 days.
3, the mouse lymphocyte transformation experiment of ConA induction
Under sterile conditions, the spleen for taking out four groups of mouse respectively is placed in and fills in sterile Hank ' s liquid plate, with 4 layers
Gauze grinds spleen, and individual cells suspension is made, and is washed 2 times with Hank ' s liquid, is centrifuged 10min every time, then cell suspends
In the complete culture solution of 1ml, is counted with Trypan Blue or cell number, adjustment cell concentration are 3 × 106A/ml, by spleen
A in two hole of cell suspension part addition, 24 well culture plates, every hole 1ml a, hole adds 75 μ l (being equivalent to 7.5 μ g/ml) ConA liquid, separately
5%CO is set as control in one hole2, cultivate 72h in 37 DEG C of carbon dioxide incubator, culture terminates preceding 4h, and every hole gently sucks
Supernatant 0.7ml is added 0.7ml and is free of the RPMI1640 culture solution of calf serum, while 50 μ l/ of MTT (5mg/ml) is added
Hole continues to cultivate 4h, and then 1ml acid isopropyl alcohol is added in every hole, and piping and druming mixes, is completely dissolved purple crystal, then dispenses
Into 96 well culture plates, each hole packing hole 3-6 measures light with enzyme-linked immunosorbent assay instrument as Duplicate Samples with 570nm wavelength
Density value, calculates the competence for added value of lymphocyte, and concrete outcome see the table below 1.Wherein, the competence for added value of lymphocyte=plus
The OD value in the hole ConA is not added in the OD value-in the hole ConA.
The competence for added value measurement result of 1 lymphocyte of table
It can be seen from the data in Table 1 that the soft capsule (C group) of embodiment 1 can increase mouse lymphocyte conversion capability,
C group has conspicuousness (P < 0.05) compared with A group, B group and D group.
4, antibody-producting cell detection
De- fiber sheep blood is taken, with brine 3 times, (2000r/min) 10min is centrifuged every time, counts cell, it will
Hematocrit SRBC is made into the cell suspension of 2% (v/v) with physiological saline, and 0.2ml is injected intraperitoneally in every mouse.Mouse cervical vertebra is taken off after 5 days
Mortar is put to death, and is taken spleen, is ground spleen with 4 layers of gauze, individual cells suspension is made, is washed 2 times with Hank ' s liquid, is centrifuged every time
Cell is finally suspended in 5ml RPMI1640 culture solution by 10min.Cell is counted, and cell concentration is adjusted to 5 × l06
A/mL.It after surface layer culture medium is dissolved by heating, is mixed with the Hanks liquid of 2 times of concentration of the pH=7.4 of equivalent, dispenses lab scale
Pipe, every pipe 0.5ml, then the 10%SRBC50ul (v/v), 20ul splenocyte suspension (5 × l0 prepared with SA liquid is added into pipe6
A/mL), it is poured on the slide of brush thin layer agarose after mixing rapidly, flat buckle of slide is placed on glass after agarose solidification
It on horse, is put into carbon dioxide incubator and incubates 1.5h, will be added in slide groove with the diluted complement of SA liquid (1:8),
Continue to count hemolysis plaque number after incubating 1.5h.Four groups of drugs (use mouse antibodies cellulation testing result as shown in table 2
Plaque number/106A splenocyte indicates).
2 antibody-producting cell detection result of table
| Group | Hemolysis plaque number (a/106A splenocyte) x ± s |
| A | 189±22 |
| B | 203±20 |
| C | 265±17 |
| D | 234±18 |
Data are found out from table 2, and the soft capsule (C group) of embodiment 1 can obviously increase the antibody-producting cell number of mouse,
C group has conspicuousness (P < 0.05) compared with A group, B group and D group.
5, mouse carbonic clearance test
By india ink stoste with 3-4 times of normal saline dilution, diluted india ink is injected from mouse tail vein
0.2ml injects, timing immediately to prepared Chinese ink.2min (t after injection prepared Chinese ink1) and 10min (t2) when, it is taken respectively from angular vein clump
20 μ l of blood, and be added in the sodium carbonate liquor of 2ml0.1% immediately, with spectrophotometer, in 600nm wavelength, measurement light is close out
Angle value is respectively OD1And OD2, mouse is put to death, liver and spleen is taken out, blots surface blood stains with filter paper, is weighed.Referred to phagocytosis
Number indicates the ability of mouse carbonic clearance, and concrete outcome see the table below 3, wherein the following formula of the calculating of phagocytic index a:
3 mouse carbonic clearance capacity experimental result of table
| Group | Phagocytic index (x ± s) |
| A | 4.42±0.86 |
| B | 4.55±0.75 |
| C | 4.99±0.53 |
| D | 4.76±0.64 |
Data are found out from table 3, and the soft capsule (C group) of embodiment 1 can obviously increase mouse carbonic clearance ability, C group with
A group, B group and D group are compared with conspicuousness (P < 0.05).
6, the detection of NK cell function
Under sterile conditions, the spleen for taking out four groups of mouse respectively is placed in and fills in sterile Hank ' s liquid plate, uses tweezers
Spleen is ground, individual cells suspension is made, is washed 2 times with Hank ' s liquid, is centrifuged 10min every time, abandons supernatant for cytoplasm bullet
It rises, 0.5ml aqua sterilisa is added, adds 0.5ml2 times of Hank ' s liquid and 8mlHank ' s liquid after splitting erythrocyte, is centrifuged
L0min, the RPMI1640 complete culture solution with lml containing 10% calf serum is resuspended, with platform phenol orchid dyeing counting (viable count
95% or more), adjustment cell concentration is 2 × 107This is effector cell to a/ml, takes well-grown YAC-1 for 24 hours after passage
It is 4 × 10 that cell RPMI1640 complete culture solution, which adjusts cell concentration,5A/ml this be target cell；Take target cell and effect thin
Each l00 μ l of born of the same parents (effect target ratio 50:1), is added in U-shaped 96 well culture plate；Target cell Spontaneous release hole adds target cell and culture solution each
L00 μ l, target cell maximum relief hole add target cell and each l00 μ l of 2.5%Triton, and above-mentioned items are all provided with three parallel holes,
37 DEG C, 4h is cultivated in 5% carbon dioxide incubator, 96 well culture plates is then centrifuged 5min with 1500r/min, every hole is drawn
In 100 μ l horizontalization bottom of supernatant, 96 well culture plate, while 100 μ l of LDH matrix liquid is added, according to room temperature reaction 3-10min, every hole
The 30 μ l of HC1 of l mol/L is added, measures optical density (OD) at microplate reader 490nm.
NK cell activity=[(reacting hole OD- Spontaneous release hole OD)/(maximum relief hole OD- Spontaneous release hole OD)] ×
100%.Four groups of drugs are active on NK cells in mice to be influenced as shown in table 4.
The testing result of 4 NK cell function of table
It can be seen from the data in Table 4 that the soft capsule (C group) of embodiment 1 can obviously increase NK cells in mice activity, C
Group has conspicuousness (P < 0.05) compared with A group, B group and D group.
Can be seen that from the above experimental data is had very using Chinese medicinal soft capsule prepared by process provided by the invention
The effect of good raising immunity.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (9)
1. a kind of Chinese medicinal soft capsule preparation process that immunity can be improved, which comprises the steps of:
(1) preparation of ganoderma Lucidum spore essential oil: in parts by weight, take 800-1200 parts of progress carbon dioxide of lucidum spore powder super
Supercritical fluid extraction obtains ganoderma Lucidum spore essential oil；
(2) preparation of Reishi sporule medicinal powder: in step (1), remaining Reishi sporule residue is with dilute after extracting ganoderma Lucidum spore essential oil
Hydrochloric acid solution impregnates 1.5 hours under ultrasonic wave effect, is then centrifuged for, then dilute hydrochloric acid solution is added in Reishi sporule residue and exists
Ultrasonic wave effect is lower to impregnate 1.5 hours, merges the solution after impregnating twice, and filtering, vacuum drying, crushes at concentration, obtains ganoderma lucidum
Spore medicinal powder；
(3) preparation of ginseng, wilsonii and Radix Astragali mixed powder: in parts by weight, by 400-600 parts of ginseng, wilsonii
Ethyl alcohol progress first time heating and refluxing extraction 1.5 hours is added, then by the in 400-600 parts and Radix Astragali 800-1000 part choppings
After the solution of refluxing extraction filters out, adds ethyl alcohol and carry out second heating and refluxing extraction 1.5 hours, merging flows back twice
The solution of extraction, filtering, vacuum drying, crush at concentration, obtain ginseng, wilsonii and Radix Astragali mixed powder；
(4) preparation of Medicinal powder of Rhodiola rosea L.: in parts by weight, 800-1000 parts of root of kirilow rhodiola being shredded, and ethyl alcohol is added and carries out the
It primary heating refluxing extraction 1.5 hours, after then filtering out the solution of first time refluxing extraction, adds ethyl alcohol and carries out second
Heating and refluxing extraction 1.5 hours, merge the solution of refluxing extraction twice, filtering, vacuum drying, crushes at concentration, obtains root of kirilow rhodiola
Medicinal powder；
(5) preparation of capsule material: ganoderma Lucidum spore essential oil obtained in step (1) is heated to 40-50 DEG C, in the case of stirring
Sequentially add the Reishi sporule medicinal powder of step (2), the mixed powder of step (3), step (4) Medicinal powder of Rhodiola rosea L., stir evenly,
Medicinal plant oil is added, keeps the temperature and stirs evenly at 40-50 DEG C, colloid mill is crossed twice, sieves with 100 mesh sieve, then carry out high pressure
Homogenization, vacuumize process obtain capsule material；
(6) soft capsule that content is 0.55g is made in capsule material, cooling, whole grain, washing, drying are to get the Chinese medicine flexible glue
Capsule.
2. a kind of Chinese medicinal soft capsule preparation process that immunity can be improved according to claim 1, which is characterized in that according to
Parts by weight meter, 1000 parts of the lucidum spore powder, 500 parts of the ginseng, 1000 parts of the Radix Astragali, 500 parts of the wilsonii,
It is 1000 parts of the root of kirilow rhodiola, medicinal plant oil 200-300 parts described.
3. a kind of Chinese medicinal soft capsule preparation process that immunity can be improved according to claim 2, which is characterized in that described
Medicinal plant oil is one of sesame oil, olive oil, sunflower oil, corn oil and grape seed oil or a variety of mixing.
4. a kind of Chinese medicinal soft capsule preparation process that immunity can be improved according to claim 1, which is characterized in that step
(1) in, the extraction temperature of the co_2 supercritical fluid extraction is 40-50 DEG C, CO2Fluid pressure is 15-25Mpa, CO2
Flow is 15-25L/h, extraction time 60-90min.
5. a kind of Chinese medicinal soft capsule preparation process that immunity can be improved according to claim 1, which is characterized in that step
(2) in, the weight ratio of Reishi sporule residue and dilute hydrochloric acid is 1:5, and the pH of the dilute hydrochloric acid is 5-6, the immersion of the dilute hydrochloric acid
Temperature is 40-50 DEG C.
6. a kind of Chinese medicinal soft capsule preparation process that immunity can be improved according to claim 1, which is characterized in that step
(3) in, the ethyl alcohol is the ethyl alcohol of content 70%, ginseng, wilsonii and Radix Astragali after the chopping of when first time refluxing extraction
Total weight and the weight ratio of 70% ethyl alcohol be 1:4, ginseng, wilsonii and Radix Astragali after the chopping of when second of refluxing extraction
Total weight and 70% ethyl alcohol weight ratio be 1:5.
7. a kind of Chinese medicinal soft capsule preparation process that immunity can be improved according to claim 1, which is characterized in that step
(4) in, the ethyl alcohol is the ethyl alcohol of content 90%, root of kirilow rhodiola weight and 90% second after the chopping of when first time refluxing extraction
Alcohol weight ratio is 1:4, and the root of kirilow rhodiola weight and 90% ethyl alcohol weight ratio after the chopping of when second of refluxing extraction are 1:5.
8. a kind of Chinese medicinal soft capsule preparation process that immunity can be improved according to claim 1, which is characterized in that step
(2), in step (3) and step (4), for the concentration process using vacuum distillation, thickening temperature is 40-60 DEG C, and concentration pressure is
10-50Kpa；The vacuum drying temperature is 40-60 DEG C.
9. a kind of Chinese medicinal soft capsule preparation process that immunity can be improved according to claim 1, which is characterized in that step
(5) in, pressure condition when described high-pressure homogeneous is 5-20Mpa, and the temperature of the vacuumize process is 0-5 DEG C, described to take out very
The pressure of vacancy reason is 1-5Kpa.
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| CN112022896A (en) * | 2020-08-09 | 2020-12-04 | 上海圣赐康药业有限公司 | Anti-tumor traditional Chinese medicine compound soft capsule preparation and preparation method and application thereof |
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| CN112022896A (en) * | 2020-08-09 | 2020-12-04 | 上海圣赐康药业有限公司 | Anti-tumor traditional Chinese medicine compound soft capsule preparation and preparation method and application thereof |
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