CN112494569A - Traditional Chinese medicine composition for improving immunity and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition for improving immunity and preparation method and application thereof Download PDFInfo
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- CN112494569A CN112494569A CN202011600877.6A CN202011600877A CN112494569A CN 112494569 A CN112494569 A CN 112494569A CN 202011600877 A CN202011600877 A CN 202011600877A CN 112494569 A CN112494569 A CN 112494569A
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- ginseng
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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Abstract
The invention discloses a traditional Chinese medicine composition for improving immunity and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight: 3-5 parts of lucid ganoderma, 3-5 parts of medlar, 3-5 parts of eucommia bark, 1-2 parts of ginseng and 0.5-1 part of cervus elaphus linnaeus. The traditional Chinese medicine composition for improving the immunity of the organism has the advantages of high efficiency, safety, wide applicable crowd, mild medicine property and the like, and is suitable for being taken by people with low immunity for a long time.
Description
Technical Field
The invention relates to the field of traditional Chinese medicines, and in particular relates to a traditional Chinese medicine composition for improving immunity and a preparation method and application thereof.
Background
The "sub-health TCM clinical guidelines" published by the Chinese TCM society in 2006 indicates that "sub-health" refers to a state of a human body between health and disease. In 2016, the world health organization reports that the global sub-health population is over 60 hundred million people, accounting for 85% of the global population. The number of Chinese sub-health people accounts for 70 percent of the total population of China, about 9.5 hundred million people. A person in a sub-health state cannot meet the health standard, and is manifested by symptoms of reduced vitality and reduced function and adaptability within a certain period of time, but does not meet the clinical or sub-clinical diagnosis standard of diseases related to modern medicine. Irregular life, frequent staying up all night, smoking, drinking, high-calorie high-fat food intake, lack of exercise and the like are common reasons of sub-health. Sub-health is mainly manifested as physiological dysfunction and decline of various systems of the human body, or a decline of comprehensive physical ability, lassitude, etc. The nervous system is characterized by: dizziness, headache, insomnia, dreaminess, hypomnesis, and lassitude; the circulating system is characterized in that: palpitation, dull pain in the heart, critical hypertension, dyslipidemia; the digestive system is embodied as: loss of appetite, dull pain in the stomach, abdominal distension, dyspepsia, constipation, and the like; the respiratory system is embodied as: suffocating, shortness of breath, dry and astringent throat or feeling of blockage; the sensory system is embodied as: tinnitus, hearing loss, dry eyes, and soreness; the metabolic system is embodied as: excessive body weight and obesity; the sports system is embodied as: bradykinesia, joint movement is not flexible, muscle soreness; the endocrine system is embodied as: critical hyperthyroidism, elevated blood glucose or hypoglycemia; the comprehensive physical ability is embodied as follows: low working efficiency, easy fatigue, physical strength, cold hands and feet, weak constitution, sexual hypofunction, and accelerated natural aging.
Modern medicine believes that immunity is a natural defense mechanism of a human body, is the capability of resisting external invasion and maintaining the stability of the internal environment of the body, the reduction of immune function is a common phenomenon of a sub-health state, and the improvement of the function of an immune system is an effective means for enhancing the body to resist various uncomfortable symptoms and improve the sub-health state of the body.
The traditional Chinese medicines with the immunity enhancing effect are various in taste, such as ginseng, codonopsis pilosula, longan, bird's nest, houttuynia cordata, honeysuckle, polyporus umbellatus, folium isatidis, garlic, isatis root, cortex acanthopanacis, glossy privet fruit, gastrodia elata, lucid ganoderma, wolfberry fruit, astragalus root, epimedium herb, gynostemma pentaphylla, human placenta, mulberry, bighead atractylodes rhizome, dodder, dried ginger, gentian, pseudo-ginseng, dark plum, white paeony root, hypericum japonicum, divaricate saposhnikovia root, elsholtzia, sealwort, dandelion, wild buckwheat rhizome, poria cocos, selfheal, radix bupleuri, Chinese yam, hawthorn, fructus choerospondiatis, corious versicolor, liquorice, arillus longan, angelica, radix ophiopogonis, eucommia ulmoides, polygonum multiflorum, dutchmanspipe root, almond, shepherdspurse herb, pilose antler, wild jujube, ramulus mori, negundo chas. The traditional Chinese medicine compound preparation enables the medicinal materials to generate synergistic action through the traditional Chinese medicine compatibility theory of monarch, minister, assistant and guide, thereby achieving the effects of improving curative effect, reducing toxic and side effects and the like, and being the main development direction of the future traditional Chinese medicine compound preparation.
The immunologic function of modern medicine is closely related to the theory of physical weakness in the field of traditional Chinese medicine. The "healthy qi exists in the interior, but pathogenic qi cannot dry out; when pathogenic factors attack, qi must be deficient. Qi benefiting, blood nourishing, yin nourishing, yang supporting and the like are basic formula strategies for enhancing the immunity of the organism in the field of traditional Chinese medicine. However, the low immunity is often caused by various comprehensive factors, all links are closely connected, only certain organic organ functions are improved or mainly nourishing is achieved, only one link is improved, and the method is not suitable and cannot meet the requirements of people with low immunity. Moreover, under the guidance of different compatibility theories, numerous immunity-enhancing traditional Chinese medicine compound products generally have the defects of weak curative effect, more side effects, limited applicable population and the like, and do not have a traditional Chinese medicine compound which is efficient, safe, wide in applicable population, mild in medicine property and suitable for long-term administration. For example: the four-monarch drug decoction is a basic prescription for invigorating qi, can improve phagocytic capacity of macrophages, and is used for treating spleen and stomach qi deficiency, yin deficiency and internal heat, which are not suitable for abdominal distention; the four-ingredient decoction is a classic formula for enriching blood and regulating blood, is warm in prescription, is suitable for immunoregulation of blood deficiency type organisms and is not suitable for yin deficiency and internal heat; the Liuwei Dihuang Wan is a yin-tonifying prescription, can enhance the phagocytic capacity of mononuclear macrophages and the proliferation of T, B lymphocytes, is mainly used for treating kidney yin deficiency or liver-kidney yin deficiency, but is mostly a yin-nourishing medicine in the prescription, and is not suitable for patients with yang deficiency.
Therefore, the development of a Chinese herbal compound which is efficient, safe, wide in applicable population, mild in drug property and suitable for long-term administration and can improve the immunity of the organism is a technical problem which needs to be solved by technical personnel in the field urgently.
Disclosure of Invention
The inventor considers that the hypoimmunity belongs to the category of consumptive disease in the traditional Chinese medicine. Consumptive disease is mainly caused by deficiency of qi and blood, imbalance of yin and yang, and malnutrition of five internal organs. The five zang organs' injuries, the poor ones and the kidneys, which are the congenital origin, dominate the bone and produce essence, store true yin and live primordial yang, which is the motive force of the body.
The inventor deeply explores various consumptive disease expressions and internal reasons of people with low immunity on the basis of the traditional Chinese medicine theory, creatively uses the compatibility theory of tonifying qi, soothing the nerves, nourishing liver and kidney and promoting the production of body fluid and nourishing blood, provides an innovative formula taking ganoderma lucidum as a monarch drug, medlar and eucommia as ministerial drugs and ginseng and cervus elaphus linnaeus as an adjuvant drug, and scientifically verifies the safety and the effectiveness of the formula by applying the modern medicine immunoregulation theory and research results. Experiments prove that the traditional Chinese medicine composition for improving the immunity has the advantages of high efficiency, safety, wide applicable population, mild medicine property, suitability for long-term administration and the like.
The traditional Chinese medicine composition for improving the immunity of the organism is mainly prepared on the basis of the following conception:
ganoderma lucidum: the traditional Chinese medicine has sweet taste and neutral nature, enters kidney, liver, heart and lung channels, has the efficacy of tonifying qi and soothing nerves, and is considered as the top grade in the Shen nong Ben Cao Jing, such as 'heart qi benefiting', 'sperm calming soul', 'liver tonifying qi' and 'muscle and bone strengthening' and the like; the compendium of materia medica also records 'nourishing and strengthening' and 'prolonging life', and 'good color' after long-term use, which are the monarch drugs of the recipe.
Wolfberry fruit and eucommia bark: the medlar is sweet in taste and neutral in nature, enters liver and kidney channels, and has the effects of tonifying kidney and replenishing vital essence, nourishing liver and improving eyesight. Eucommia bark, cortex eucommiae, with sweet taste and warm nature, enters liver and kidney meridians, has the efficacies of tonifying liver and kidney and strengthening bones and muscles, is taken for a long time, is light and ageing resistant (Shennong's herbal Jing), can enter liver and kidney, tonify middle-jiao and vital energy, strengthen bones and muscles, strengthen mind, treat lumbago due to kidney deficiency, is taken for a long time, and is light and ageing resistant (Ben Cao gang mu). The medlar and the eucommia bark, which have the effects of tonifying the essence and blood of the liver and the kidney and tonifying the qi of the liver and the kidney, are the ministerial drugs of the recipe.
Human participated in cervus elaphus linnaeus: ginseng is sweet, slightly bitter and warm in taste, has the functions of invigorating primordial qi, restoring pulse, relieving depletion, invigorating spleen, benefiting lung, promoting fluid production, quenching thirst, tranquilizing mind and improving intelligence, and can be used for treating diabetes. Cornu Cervi Pantotrichum is sweet and salty in taste and warm in nature, enters liver and kidney meridians, and has effects of invigorating kidney yang, replenishing essence and blood, strengthening tendons and bones, regulating Chong and ren meridians, and eliminating sore toxin. The participation of man in cervus elaphus linnaeus is an assistant and guiding drug of the recipe for invigorating qi and tonifying yang.
The invention takes the ganoderma lucidum as the monarch drug, and has the effects of tonifying qi, soothing the nerves, nourishing and strengthening. Fructus Lycii has effects in invigorating kidney, replenishing vital essence, nourishing liver, and improving eyesight; the eucommia bark can tonify the liver and kidney and strengthen the muscles and bones; the medlar and the eucommia bark, which have the effects of tonifying the essence and blood of the liver and the kidney and tonifying the qi of the liver and the kidney, are the ministerial drugs of the invention. Ginseng is good at tonifying primordial qi and has the effects of tonifying qi and promoting the production of body fluid; cornu Cervi Pantotrichum has effects of invigorating kidney yang, and replenishing essence and blood; the human body participates in cervus elaphus linnaeus, and has the functions of invigorating qi and tonifying yang, which are the adjuvant and guiding drugs of the invention. The lucid ganoderma, the medlar, the eucommia ulmoides, the ginseng and the cervus elaphus linnaeus are combined to complement each other, and the five medicines of the lucid ganoderma, the medlar, the eucommia ulmoides, the ginseng and the cervus elaphus linnaeus are beneficial to the health of the organism and the immunity of the organism by nourishing liver and kidney, tonifying qi and strengthening spleen, promoting the production of body fluid and nourishing blood, tonifying yin and yang and qi and blood and benefiting the five internal organs and the six internal.
Based on the monarch, minister, assistant and guide theory of traditional Chinese medicine and clinical medication practice, the invention is proved by combining pharmacological research results, compared with other compatibility concepts of similarly selecting lucid ganoderma, medlar, eucommia bark, ginseng and cervus elaphus linnaeus, the traditional Chinese medicine composition which is formed under the guidance of compatibility theory of tonifying qi and soothing the nerves, nourishing liver and kidney and promoting the production of body fluid and nourishing blood and takes lucid ganoderma as monarch medicine, medlar and eucommia bark as ministerial medicine and ginseng and cervus elaphus linnaeus as assistant and guide medicine has obvious advantages in the aspects of improving the immunity of organisms, using safety, application crowd range and the like, and the invention is completed based on.
In particular, the amount of the solvent to be used,
the invention provides a traditional Chinese medicine composition, which can efficiently and safely improve the immunity of organisms, has the advantages of mild medicine property, wide applicable population, suitability for long-term taking and the like, and is prepared from the following traditional Chinese medicines in parts by weight:
wherein, the lucid ganoderma is a monarch drug, the medlar and the eucommia bark are ministerial drugs, and the ginseng and the cervus elaphus linnaeus are assistant and guide drugs.
Wherein, the cervus elaphus linnaeus is preferably cervus elaphus linnaeus powder.
Wherein, the ginseng is preferably ginseng powder.
Wherein, as the ministerial drug of the Chinese medicinal composition, the weight ratio of the medlar to the eucommia bark is preferably 1: 1.
As an adjuvant drug of the traditional Chinese medicine composition, the weight ratio of the ginseng to the cervus elaphus linnaeus is preferably 1-2: 1.
Preferably, the invention provides a traditional Chinese medicine composition for improving body immunity, which is prepared from the following traditional Chinese medicines in parts by weight:
wherein the Ganoderma is preferably dried fruiting body of Ganoderma lucidum belonging to Polyporaceae, harvesting all year round, removing impurities, cutting off lower end stipe attached with rotten wood, silt or culture medium, drying in the shade, and pre-treating (removing impurities, cleaning, and drying); selecting fructus Lycii preferably dried mature fruit of Lycium barbarum L of Solanaceae, collecting in summer and autumn when the fruit is red, air drying to wrinkle, sun drying, and removing fruit stalks; selecting Eucommiae cortex preferably from dried bark of eucommia ulmoides of Eucommiaceae, peeling at 4-6 months, removing rough bark, piling up to give purple brown inner skin, sun drying, and pre-treating (removing residual rough bark, cleaning, cutting, and drying); the Ginseng radix is preferably selected from dried root and rhizome of Panax ginseng C.A. Meyer of Araliaceae, cleaning, drying, and pre-treating (moistening, slicing, and drying); cornu Cervi Pantotrichum is young horn of male deer of Cervidae animal without ossification and dense antler hair, preferably dried and pulverized powder.
Further, the invention also relates to a preparation method of the traditional Chinese medicine composition, which comprises the following steps:
1) decocting Ganoderma, fructus Lycii and Eucommiae cortex in water, filtering decoction, concentrating the filtrate, drying, and pulverizing into mixed powder;
2) drying Ginseng radix, and pulverizing into Ginseng radix powder, or directly weighing Ginseng radix powder;
3) drying cornu Cervi Pantotrichum, and pulverizing into powder, or directly weighing cornu Cervi Pantotrichum powder;
4) and (3) uniformly mixing the mixed powder, the ginseng powder and the cervus elaphus linnaeus powder to obtain the traditional Chinese medicine composition.
Preferably, the mixed powder, the ginseng powder and the hairy antler powder all pass through a No. five sieve, and the powder which can pass through a No. six sieve is not less than 95%.
Preferably, wherein the organic solvent in step 1) is ethanol.
Preferably, the ganoderma lucidum, the medlar and the eucommia ulmoides in the step 1) are decocted by adding water for at least 2 times, 5 to 15 times of water (more preferably 10 times of water) is added for each time, the decoction is respectively decocted for 1 to 3 hours (more preferably 2 hours), the decoction is combined and filtered; wherein, the filtering step adopts a sieve with 150 meshes and 300 meshes, and more preferably a sieve with 200 meshes.
Preferably, the filtrate obtained in the step 1) is decompressed and concentrated to obtain extract, the extract is dried in vacuum to obtain dry paste, and the dry paste is crushed into mixed powder and sieved; wherein the filtrate is concentrated under reduced pressure at-0.065 to-0.075 Mpa (75 ℃), the relative density of the extract is 1.10 to 1.35(60 ℃), preferably 1.10 to 1.25(60 ℃), and more preferably 1.10 to 1.20(60 ℃); vacuum drying under-0.085 Mpa at 65 deg.C; sieving with 80 mesh sieve; the filtrate is preferably concentrated by adopting a traditional Chinese medicine concentrating tank so as to prevent the effective components from being damaged by heating for a long time.
Preferably, wherein the drying temperature of the ginseng in the step 2) is 60 ℃, the particle size of the ginseng powder is preferably 100 mesh, more preferably, the ginseng powder is subjected to radiation sterilization under the irradiation condition that the dose of 5kGy is preferable60Co。
Preferably, wherein the drying temperature of the cervus elaphus linnaeus in the step 3) is 60 ℃, the granularity of the cervus elaphus linnaeus powder is preferably 100 meshes, the cervus elaphus linnaeus powder is used after being subjected to radiation sterilization, and the radiation condition is preferably 5kGy dosage60Co。
Furthermore, the invention also relates to application of the traditional Chinese medicine composition in preparing a product with the effect of improving immunity, and the product is preferably a medicine or a health-care food.
Further, the invention also relates to a traditional Chinese medicine preparation for improving immunity, which can be prepared into capsules, granules, tablets, pills, powder or oral liquid and other dosage forms according to the conventional preparation technology in the field. For example: for capsules, the traditional Chinese medicine composition can be prepared into soft materials by using 85% ethanol, granulation is carried out by using a 16-mesh sieve, drying is carried out at 55-60 ℃, granulation is carried out by using a 14-mesh sieve, 0.35 g/capsule is filled into a No. 0 capsule, and the capsules can be prepared by polishing, inspecting and packaging.
The recommended dosage of the traditional Chinese medicine preparation capsule is as follows: orally taken 2 times/day, 3 capsules (1.05g) each time, or following the prescription.
The traditional Chinese medicine composition of the invention has the following beneficial effects:
(1) the invention takes the lucid ganoderma which can tonify qi, calm the nerves, benefit heart qi, nourish liver and tonify qi as a monarch drug and mainly attacks the deficiency of qi and blood caused by consumptive disease of the organism.
(2) The invention creatively combines the effect of nourishing liver and kidney of the monarch drug ganoderma lucidum and the ministerial drugs medlar and eucommia bark, effectively stimulates and maintains the essence and blood of the liver and kidney and the qi of the liver and kidney of the organism, and the qi and the blood of the organism can be supplemented. Particularly, the ministerial medicines of the medlar, the eucommia and the monarch medicine of the lucid ganoderma are approximately used in equal amount, water decoction and extraction are carried out together, the monarch and ministerial medicines have synergistic effect, and the technical effects of quick recovery of qi and blood deficiency of the organism and lasting effect are achieved.
(3) The ginseng and cervus elaphus linnaeus have the effects of promoting the production of body fluid and nourishing blood, and the five internal organs are further benefited, compared with monarch drugs and ministerial drugs, the dosage is nearly halved, the lucid ganoderma, the barbary wolfberry fruit and the eucommia bark are stimulated to quickly exert the effect of tonifying qi and blood, the vigor of tonifying qi and blood of the lucid ganoderma, the barbary wolfberry fruit and the eucommia bark is restricted, the diseases of imbalance of yin and yang of an organism and malnutrition of the five internal organs are relieved, and the.
(4) Scientific experiments show that the traditional Chinese medicine composition disclosed by the invention has no obvious influence on the weight, the viscera/body ratio and the carbon clearance capacity of a mouse after being orally and continuously administered to the mouse for 30 days; for delayed type allergy, antibody-producing cell number, HC50The ability of chicken erythrocytes phagocytized by macrophages, the activity of NK cells and the mouse lymphocyte transformation induced by ConA are enhanced or promoted, namely the traditional Chinese medicine composition has obvious promotion effects on cellular immunity, humoral immunity, mononuclear-macrophage function and NK cell activity of an organism, and has the function of safely and efficiently enhancing the immunity of the organism.
(5) The invention utilizes the principle of monarch, minister, assistant and guide of traditional Chinese medicine, combines the internal cause of 'consumptive disease' of low immunity of organisms, skillfully takes lucid ganoderma as monarch drug, takes medlar and eucommia as ministerial drugs and takes ginseng and cervus elaphus linnaeus as assistant and guide drugs, so that the whole formula is efficient, safe, mild in drug property, suitable for a large number of people and suitable for long-term taking of people with low immunity.
(6) The formula with other conception, such as the formula which takes ginseng and hairy antler for promoting the secretion of saliva or body fluid and nourishing blood as monarch drugs, takes ganoderma lucidum for tonifying qi and soothing nerves as ministerial drugs, and takes medlar and eucommia bark for nourishing liver and kidney as assistant drugs (comparative example 1); the formula (comparative example 2) takes the medlar for nourishing the liver and kidney as the monarch drug, the ginseng for promoting the production of body fluid and nourishing the blood as the ministerial drug, the lucid ganoderma for tonifying qi and soothing the nerves and the eucommia bark for nourishing the liver and kidney as the adjuvant drug and the cervus elaphus linnaeus for promoting the production of body fluid and nourishing the blood as the messenger drug; compared with the formula (comparative example 3) which takes the barbary wolfberry fruit and the eucommia bark which can nourish the liver and the kidney as monarch drugs, the lucid ganoderma which can tonify qi and calm the nerves and the hairy antler which can promote the production of body fluid and nourish blood as ministerial drugs and the ginseng which can promote the production of body fluid and nourish blood as adjuvant drugs, the traditional Chinese medicine composition has obvious advantages in the aspects of medication safety, effectiveness, application crowd range and the like. Namely, compared with other prescription strategies or concepts, the prescription mainly has the effects of tonifying qi and soothing the nerves, nourishing the liver and kidney, and promoting the production of body fluid and nourishing the blood (examples 1 to 3) is more effective in improving the immunity of the organism and has a wider application range.
(7) Ginseng and hairy antler are rare and have good powder forming property. The traditional Chinese medicine composition is prepared from powder, can be used as an excipient, avoids moisture absorption and agglomeration of capsule contents, and can retain the effective components to the maximum extent; not only improves the stability of the product, but also reduces the use of auxiliary materials and reduces the production cost.
Detailed Description
The technical solution of the present invention will be described in detail with reference to specific examples and comparative examples. The traditional Chinese medicine raw materials used by the invention are purchased from Xijingzhi medicine Limited company of Xian algae Lutang pharmaceutical industry group.
EXAMPLE 1 preparation of the Chinese medicinal composition of the present invention
(1) Decocting Ganoderma, fructus Lycii, and cortex Eucommiae in water
Decocting 5.0kg of lucid ganoderma, 3.5kg of medlar and 3.5kg of eucommia bark in water for 2 times, adding 10 times of water each time, decocting for 2 hours respectively, filtering decoction liquid (a stainless steel sieve of 200 meshes), merging filtrate, concentrating the filtrate under reduced pressure (-0.065-0.075 Mpa at 75 ℃) to obtain extract with the relative density of 1.30-1.35 (60 ℃), drying the extract under vacuum (-0.085Mpa at 65 ℃) to obtain dry extract, crushing the dry extract, and sieving the dry extract by a sieve of 80 meshes to obtain mixed powder.
(2) Drying and pulverizing Ginseng radix
1.5kg of ginseng is taken, dried at 60 ℃, crushed, sieved by a 100-mesh sieve, sterilized by irradiation (5kGy,60co) to obtain the ginseng powder.
(3) Drying cornu Cervi Pantotrichum, and pulverizing
1.0kg of cervus elaphus linnaeus is taken, dried at 60 ℃, crushed, sieved by a 100-mesh sieve, irradiated and sterilized (5kGy,60co) to obtain the antler powder.
(4) Chinese medicinal composition
Mixing the mixed powder, the ginseng powder and the hairy antler powder evenly to obtain 4.50kg of the traditional Chinese medicine composition.
EXAMPLE 2 preparation of the Chinese medicinal composition of the present invention
(1) Decocting Ganoderma, fructus Lycii, and cortex Eucommiae in water
Decocting 5.0kg of lucid ganoderma, 3.5kg of medlar and 4.5kg of eucommia bark in water for 3 times, adding 10 times of water each time, decocting for 2 hours respectively, filtering decoction liquid (a stainless steel sieve of 200 meshes), merging filtrate, concentrating the filtrate under reduced pressure (-0.065-0.075 Mpa at 75 ℃) to obtain extract with the relative density of 1.10-1.20 (60 ℃), drying the extract under vacuum (-0.085Mpa at 65 ℃) to obtain dry extract, crushing the dry extract, and sieving the dry extract by a sieve of 80 meshes to obtain mixed powder.
(2) Drying and pulverizing Ginseng radix
1.0kg of ginseng is taken, dried at 60 ℃, crushed, sieved by a 100-mesh sieve, sterilized by irradiation (5kGy,60co) to obtain the ginseng powder.
(3) Drying cornu Cervi Pantotrichum, and pulverizing
0.5kg of cervus elaphus linnaeus is taken, dried at 60 ℃, crushed, sieved by a 100-mesh sieve, irradiated and sterilized (5kGy,60co) to obtain the antler powder.
(4) Chinese medicinal composition
Mixing the mixed powder, the ginseng powder and the hairy antler powder evenly to obtain 3.37kg of the traditional Chinese medicine composition.
EXAMPLE 3 preparation of the Chinese medicinal composition of the present invention
(1) Decocting Ganoderma, fructus Lycii, and cortex Eucommiae in water
Decocting 4.5kg of lucid ganoderma, 4.5kg of medlar and 3.5kg of eucommia bark in water for 2 times, adding 10 times of water each time, decocting for 2 hours respectively, filtering decoction liquid (a stainless steel sieve of 200 meshes), merging filtrate, concentrating the filtrate under reduced pressure (-0.065-0.075 Mpa at 75 ℃) to obtain extract with the relative density of 1.20-1.25 (60 ℃), drying the extract under vacuum (-0.085Mpa at 65 ℃) to obtain dry extract, crushing the dry extract, and sieving the dry extract by a sieve of 80 meshes to obtain mixed powder.
(2) Ginseng radix powder and cornu Cervi Pantotrichum powder
1.0kg of ginseng powder and 1.0kg of cervus elaphus linnaeus powder are taken, sieved by a 100-mesh sieve, irradiated and sterilized (5kGy,60Co)。
(3) chinese medicinal composition
Mixing the mixed powder, the ginseng powder and the hairy antler powder evenly to obtain 3.95kg of the traditional Chinese medicine composition.
EXAMPLE 4 preparation of the Chinese medicinal composition of the present invention
(1) Decocting Ganoderma, fructus Lycii, and cortex Eucommiae in water
Decocting 3.5kg of lucid ganoderma, 4.0kg of medlar and 4.0kg of eucommia bark with water for 3 times, adding 10 times of water each time, decocting for 2 hours respectively, filtering decoction liquid (a stainless steel sieve of 200 meshes), merging filtrate, concentrating the filtrate under reduced pressure (-0.065-0.075 Mpa at 75 ℃) to obtain extract with the relative density of 1.10-1.20 (60 ℃), drying the extract under vacuum (-0.085Mpa at 65 ℃) to obtain dry extract, crushing the dry extract, and sieving the dry extract with a sieve of 80 meshes to obtain mixed powder.
(2) Ginseng radix powder and cornu Cervi Pantotrichum powder
2.0kg of ginseng powder and 1.0kg of cervus elaphus linnaeus powder are taken, sieved by a 100-mesh sieve, irradiated and sterilized (5kGy,60Co)。
(3) chinese medicinal composition
Mixing the mixed powder, the ginseng powder and the hairy antler powder evenly to obtain 4.71kg of the traditional Chinese medicine composition.
Example 5 preparation of capsules of the Chinese medicinal composition of the present invention
Taking 4.5kg of the traditional Chinese medicine composition prepared in the embodiment 1, preparing a soft material by using 85% ethanol, granulating by using a 16-mesh sieve, drying at 55-60 ℃, grading by using a 14-mesh sieve, filling 0.35 g/capsule into a No. 0 capsule, polishing, checking and packaging to prepare the traditional Chinese medicine composition capsule 11712 granules.
Control example 1 preparation of control Chinese medicinal composition
(1) Decocting Ginseng radix and cornu Cervi Pantotrichum with water
Decocting 4.5kg of ginseng and 4.5kg of cervus elaphus linnaeus with water for 2 times, adding 10 times of water each time, decocting for 2 hours respectively, filtering decoction liquid (200-mesh stainless steel sieve), merging filtrate, concentrating under reduced pressure (-0.065-0.075 Mpa, 75 ℃) to obtain extract with the relative density of 1.30-1.35 (60 ℃), drying under vacuum (-0.085Mpa, 65 ℃) to obtain dry extract, crushing, and sieving by a 80-mesh sieve to obtain mixed powder of ginseng and cervus elaphus linnaeus.
(2) Drying and pulverizing Ganoderma
Drying Ganoderma 3.0kg at 60 deg.C to obtain powderCrushed, sieved through a 100 mesh sieve, sterilized by irradiation (5kGy,60co) to obtain the ganoderma lucidum powder.
(3) Drying and pulverizing fructus Lycii and Eucommiae cortex
Drying fructus Lycii 1.0kg and Eucommiae cortex 1.5kg at 60 deg.C, pulverizing, sieving with 100 mesh sieve, sterilizing by irradiation (5kGy,60co) to obtain the mixed powder of the medlar and the eucommia bark.
(4) Chinese medicinal composition
Mixing Ginseng radix with cornu Cervi Pantotrichum powder, Ganoderma powder, fructus Lycii and Eucommiae cortex powder to obtain control Chinese medicinal composition 6.57 kg.
Control example 2 preparation of control Chinese medicinal composition
(1) Decocting fructus Lycii and Ginseng radix in water
5.0kg of medlar and 4.0kg of ginseng are taken, water is added for decocting for 3 times, 10 times of water is added for each time, the decoction is respectively decocted for 2 hours, decoction liquid is filtered (a 200-mesh stainless steel sieve), filtrate is combined, decompression concentration is carried out to obtain extract with the relative density of 1.20-1.25 (60 ℃), dry extract is obtained after vacuum drying is carried out to obtain dry extract with the relative density of (0.065-0.075 Mpa, 75 ℃), the dry extract is crushed, and the dry extract is sieved by a 80-mesh sieve to obtain medlar and ginseng mixed powder.
(2) Drying and pulverizing Ganoderma and Eucommiae cortex
Drying Ganoderma 2.0kg and Eucommiae cortex 2.0kg at 60 deg.C, pulverizing, sieving with 100 mesh sieve, sterilizing by irradiation (5kGy,60co) to obtain the ganoderma lucidum and eucommia bark powder.
(3) Drying cornu Cervi Pantotrichum, and pulverizing
1.5kg of cervus elaphus linnaeus is taken, dried at 60 ℃, crushed, sieved by a 100-mesh sieve, irradiated and sterilized (5kGy,60co) to obtain the antler powder.
(4) Chinese medicinal composition
Mixing fructus Lycii and Ginseng radix mixed powder, Ganoderma and Eucommiae cortex powder, and cornu Cervi Pantotrichum powder to obtain control Chinese medicinal composition 6.62 kg.
Control example 3 preparation of control Chinese medicinal composition
(1) Decocting fructus Lycii and cortex Eucommiae in water
Decocting 4.0kg of barbary wolfberry fruit and 5.0kg of eucommia bark in water for 2 times, adding 10 times of water each time, decocting for 2 hours, filtering decoction liquid (a 200-mesh stainless steel sieve), merging filtrate, concentrating under reduced pressure (-0.065-0.075 Mpa, 75 ℃) to obtain extract with the relative density of 1.10-1.20 (60 ℃), drying under vacuum (-0.085Mpa, 65 ℃) to obtain dry extract, crushing, and sieving by a 80-mesh sieve to obtain mixed powder of barbary wolfberry fruit and eucommia bark.
(2) Drying and pulverizing Ganoderma and cornu Cervi Pantotrichum
2.0kg of lucid ganoderma and 2.5kg of cervus elaphus linnaeus are taken, dried and crushed at 60 ℃, sieved by a 100-mesh sieve, irradiated and sterilized (5kGy,60co) to obtain the mixed powder of the glossy ganoderma and the hairy antler.
(3) Drying and pulverizing Ginseng radix
1.0kg of ginseng is taken, dried at 60 ℃, crushed, sieved by a 100-mesh sieve, sterilized by irradiation (5kGy,60co) to obtain the ginseng powder.
(4) Chinese medicinal composition
Mixing fructus Lycii and Eucommiae cortex powder, Ganoderma and cornu Cervi Pantotrichum powder, and Ginseng radix powder to obtain control Chinese medicinal composition 6.84 kg.
Safety and effectiveness experimental method for traditional Chinese medicine composition
384 Balb/C mice (provided by the Experimental animals center of the military medical science institute of the people's liberation force, China, with the certification number of 0008159, SPF level, half male and female, and the body weight of 18g-22g) are randomly divided into 4 batches (96 mice per batch), and each batch is randomly divided into 8 groups (12 mice per group), namely 4 experimental groups, 3 control groups and 1 blank control group are arranged per batch. 30 days after continuous gavage, the test of organ/body weight ratio and mouse clearance was carried out in 1 st batch, and delayed type allergy, antibody-producing cell assay, HC test and the like were carried out in 2 nd batch50Measurement, in batch 3, chicken erythrocyte phagocytosis by mouse abdominal macrophages, and in batch 4, ConA-induced lymphocyte transformation and NK cell activity in mice were measured.
The preparation method, administration mode, dosage and the like of the test solution of each group are shown in the following table:
experimental example 1 Effect of the Chinese medicinal composition of the present invention on body weight and organ/body weight ratio of mouse
The initial weight and the final weight of the mice in the experimental group, the control group and the blank control group are balanced, and the traditional Chinese medicine composition has no obvious influence on the weight of the mice, and is detailed in the following tables 1-2.
TABLE 1 initial body weights of the groups of mice
| Group of | Number of mice | Batch 1 | Batch 2 | Batch 3 | Batch 4 |
| Experimental group 1 | 12 pieces of | 19.3±0.9g | 19.0±0.7g | 19.3±1.1g | 19.3±1.0g |
| Experimental group 2 | 12 pieces of | 19.4±1.3g | 19.3±0.9g | 19.2±1.0g | 19.0±0.8g |
| Experimental group 3 | 12 pieces of | 19.3±1.1g | 19.2±1.0g | 20.0±1.1g | 19.4±0.9g |
| Experimental group 4 | 12 pieces of | 19.3±0.7g | 19.1±1.2g | 20.1±1.0g | 19.6±1.0g |
| Control group 1 | 12 pieces of | 19.4±0.8g | 19.1±0.8g | 19.6±0.8g | 19.8±1.1g |
| Control group 2 | 12 pieces of | 19.5±1.0g | 19.3±0.8g | 19.2±1.0g | 19.3±0.9g |
| Control group 3 | 12 pieces of | 19.3±1.2g | 19.1±1.1g | 19.5±0.9g | 19.7±0.6g |
| Blank control group | 12 pieces of | 19.3±0.9g | 19.1±0.5g | 19.6±1.2g | 19.5±0.6g |
TABLE 2 terminal body weights of the groups of mice
Weighing the mice, dislocating and killing the mice, taking the spleen and the thymus, sucking dry surface blood stains by using filter paper, weighing the blood stains, and calculating the ratio of the spleen to the body weight and the ratio of the thymus to the body weight. The results show that the ratio of the mouse viscera/body of the experimental group, the control group and the blank control group has no obvious difference, and the traditional Chinese medicine composition has no obvious influence on the ratio of the mouse viscera/body, and is detailed in the following table 3.
TABLE 3 Effect of the Chinese medicinal composition of the present invention on the ratio of visceral organs/body weight of mice
| Group of | Number of mice | Spleen/body weight (mg/g) | Thymus/body weight (mg/g) |
| Experimental group 1 | 12 pieces of | 3.46±0.35 | 2.10±0.31 |
| Experimental group 2 | 12 pieces of | 3.43±0.27 | 2.04±0.18 |
| Experimental group 3 | 12 pieces of | 3.27±0.19 | 2.37±0.26 |
| Experimental group 4 | 12 pieces of | 3.35±0.19 | 2.35±0.25 |
| Control group 1 | 12 pieces of | 3.36±0.25 | 2.15±0.21 |
| Control group 2 | 12 pieces of | 3.40±0.20 | 2.10±0.15 |
| Control group 3 | 12 pieces of | 3.37±0.15 | 2.27±0.20 |
| Blank control group | 12 pieces of | 3.48±0.29 | 2.21±0.51 |
Experimental example 2 Effect of the Chinese medicinal composition of the present invention on carbon clearance ability of mice
Injecting Indian ink diluted by 1:4 into mouse tail vein, calculated by 0.1ml per 10g body weight, and immediately timing when the ink is injected. 20 μ l of blood was taken from the angular venous plexus 2, 10min after the ink injection and immediately added to 2ml of 0.1% sodium carbonate solution. The Optical Density (OD) was determined with a semi-automatic biochemical analyzer at a wavelength of 600nm, with a sodium carbonate solution as a blank. Mice were sacrificed, livers and spleens were removed, blood stains on the surfaces of the organs were blotted with filter paper, weighed, and phagocytic index a was calculated according to the following formula:
K＝(lgOD1-lgOD2)/(t2-t1) A ═ body weight ÷ (liver weight + spleen weight) × K1/3
The results show that the carbon clearance capacity (phagocytosis index a) of the control group mice is superior to that of the blank control group, but is obviously weaker than that of the experimental group, and the carbon clearance capacity of the experimental group mice is obviously superior to that of the control group and the blank control group. It is expected that the Chinese medicinal composition of the present invention can significantly enhance the nonspecific immune function of the body, as detailed in table 4 below.
TABLE 4 Effect of the Chinese medicinal composition of the present invention on mouse carbon clearance (phagocytic index a)
Experimental example 3 Effect of the Chinese medicinal composition of the present invention on delayed type allergy ability in mice
Sheep positive erythrocytes induced mouse DTH (plantar thickening method): sheep blood was collected, washed 3 times with physiological saline (2000r/min, 10 min), each mouse was intraperitoneally injected with 2% (v/v, formulated with physiological saline) of packed SRBC 0.2ml, 4 days after sensitization, left hind plantar thickness was measured, the same site was measured three times, and an average value was taken. The extent of DTH was then expressed as the difference in plantar thickness of the left hind foot 24 hours after injection of 20% (v/v, in saline) of packed SRBC 20 μ l subcutaneously at the site of measurement.
The result shows that the swelling degree of 24h foot sole of the control group mouse is higher than that of the blank control group, while the swelling degree of 24h foot sole of the experimental group mouse is obviously higher than that of the control group and the blank control group, and the traditional Chinese medicine composition has an enhancement effect on the delayed type allergic reaction capability of the mouse, and is detailed in the following table 5.
TABLE 5 Effect of the Chinese medicinal composition of the present invention on Sheep Red Blood Cell (SRBC) induced mouse DTH
| Group of | Number of mice | Swelling degree of foot sole 24h (mm) |
| Experimental group 1 | 12 pieces of | 0.31±0.09 |
| Experimental group 2 | 12 pieces of | 0.30±0.07 |
| Experimental group 3 | 12 pieces of | 0.32±0.12 |
| Experimental group 4 | 12 pieces of | 0.34±0.10 |
| Control group 1 | 12 pieces of | 0.25±0.05 |
| Control group 2 | 12 pieces of | 0.28±0.14 |
| Control group 3 | 12 pieces of | 0.23±0.10 |
| Blank control group | 12 pieces of | 0.21±0.09 |
Experimental example 4 Effect of the Chinese medicinal composition of the present invention on mouse antibody-producing cells
Antibody-producing cell assay (Jerne modified slide method): preparing 2% cell suspension of packed SRBC with normal saline, injecting 0.2ml into abdominal cavity of each mouse for immunization, dislocating cervical vertebra of the mouse after five days, taking out spleen, placing in a grinder containing Hank's solution, slightly grinding spleen to prepare cell suspension, filtering through a 200-mesh screen, centrifuging (1000r/min) for 10min, washing with Hank's solution for 2 times, and finally suspending the cells in 8ml RPMI 1640 culture solution. Heating and dissolving a surface layer culture medium (1g of agarose added with double distilled water to 100ml), placing the mixture into a 45 ℃ water bath for heat preservation, mixing the mixture with an equal amount of Hank's solution with the pH value of 7.2-7.4 and the concentration of 2 times, subpackaging small test tubes, wherein each tube is 0.5ml, adding 50 mu 110% SRBC (v/v prepared by SA solution) and 25 mu l of spleen cell suspension into the tubes, quickly mixing the mixture evenly, pouring the mixture onto a slide with a brushed agarose thin layer to form a parallel sheet, horizontally buckling the slide on a slide frame after the agar is solidified, placing the slide on a carbon dioxide incubator for 1.5h, adding a complement (1:6) diluted by SA buffer solution into a groove of the slide frame, continuing incubation for 1.5h, and counting the number of hemolytic plaques.
The result shows that the number of the hemolytic plaques of the control group mouse is obviously higher than that of the blank control group, while the number of the hemolytic plaques of the experimental group mouse is obviously higher than that of the control group and the blank control group, and the traditional Chinese medicine composition has an obvious promotion effect on the generation of the antibody generating cells of the mouse. It is expected that the Chinese medicinal composition of the present invention can significantly improve the humoral immune function of the body, and the details are shown in the following table 6.
TABLE 6 Effect of the Chinese medicinal composition of the present invention on the number of antibody-producing cells
| Group of | Number of mice | Number of hemolytic plaques (one) |
| Experimental group 1 | 12 pieces of | 166.5±30.9 |
| Experimental group 2 | 12 pieces of | 153.6±25.8 |
| Experimental group 3 | 12 pieces of | 154.9±30.2 |
| Experimental group 4 | 12 pieces of | 158.3±28.6 |
| Control group 1 | 12 pieces of | 122.6±22.6 |
| Control group 2 | 12 pieces of | 133.6±24.4 |
| Control group 3 | 12 pieces of | 124.9±31.8 |
| Blank control group | 12 pieces of | 103.0±15.3 |
Experimental example 5 Effect of the Chinese medicinal composition of the present invention on half-maximal hemolytic value of mouse
Half maximal hemolysis value (HC)50) The determination of (1): preparing 2% cell suspension from packed SRBC with normal saline, injecting 0.2ml into abdominal cavity of each mouse for immunization, taking blood from eyeball after five days, and separating serum. The serum was diluted 300-fold with SA buffer, and 1ml of the diluted serum was taken, and 0.5ml of 10% SRBC and 1ml of complement were added in this order (diluted 1:6 with SA buffer), and a control tube without serum was provided (replaced with SA buffer). Placing the mixture in a thermostatic water bath at 37 ℃ for 25min, and stopping the reaction in the ice bath. Centrifuging at 2000r/min for 10min, collecting supernatant 1ml, placing Dushi agent 3ml in one test tube, simultaneously collecting 10% SRBC (v/v)0.25ml, adding Dushi agent to 4ml in another test tube, mixing well, standing for 10min, and measuring optical density value of each tube at 540 nm.
Sample HC50(optical density value of sample/optical density value at half-maximal hemolysis of SRBC) × dilution factor
The results show that HC50 of the mice in the experimental group is obviously higher than that of the control group and the blank control group, and the traditional Chinese medicine composition has an effect of increasing the half hemolysis value of the mice, and is detailed in the following table 7.
TABLE 7 Chinese medicinal composition of the present invention on mouse serum hemolysin (half hemolysis value HC)50) Influence of (2)
| Group of | Number of mice | HC50 |
| Experimental group 1 | 12 pieces of | 187.7±19.3 |
| Experimental group 2 | 12 pieces of | 190.2±19.6 |
| Experimental group 3 | 12 pieces of | 196.7±26.9 |
| Experimental group 4 | 12 pieces of | 192.3±22.5 |
| Control group 1 | 12 pieces of | 169.9±16.7 |
| Control group 2 | 12 pieces of | 160.2±17.6 |
| Control group 3 | 12 pieces of | 176.7±25.4 |
| Blank control group | 12 pieces of | 140.6±24.7 |
Experimental example 6 influence of the Chinese medicinal composition on phagocytosis of chicken red blood cells by macrophages in abdominal cavity of mouse
Mouse abdominal macrophage phagocytic chicken erythrocyte test (in vitro method): 4 days before the experiment, each mouse was injected with 0.2ml of 2% packed sheep red blood cells. Killing mice by cervical dislocation, injecting 5 ml/mouse Hank's solution containing 5% calf serum into the mice by intraperitoneal injection, gently rubbing the abdomen for 20 times, then extracting 2ml of abdominal cavity cleaning solution, taking 0.5ml of abdominal cavity cleaning solution, adding the Hank's solution into a test tube containing 0.5ml of 1% chicken RBC suspension prepared, uniformly mixing, taking 0.5ml of suspension, placing the smear into a porcelain plate padded with wet gauze, placing the porcelain plate in a 37 ℃ incubator for 25min, taking out, washing the back of the glass slide by using physiological saline, fixing, dyeing and reading the slide. Phagocytosis rate and phagocytosis index were calculated.
The results show that the phagocytic percentage and the phagocytic index of the chicken erythrocytes phagocytosed by the macrophages of the mice in the control group and the blank control group are not obviously different, while the phagocytic percentage and the phagocytic index of the chicken erythrocytes phagocytosed by the macrophages of the mice in the experimental group are obviously higher than those of the control group and the blank control group, and the traditional Chinese medicine composition has an obvious effect of increasing the chicken erythrocyte phagocytosis capability of the macrophages of the mice, and is detailed in the following table 8.
TABLE 8 Effect of the Chinese medicinal composition of the present invention on the ability of macrophages in mouse abdominal cavity to phagocytose chicken red blood cells
| Group of | Number of mice | Phagocytosis ratio (%) | Phagocytic index |
| Experimental group 1 | 12 pieces of | 31.7±2.3 | 0.44±0.04 |
| Experimental group 2 | 12 pieces of | 36.5±3.7 | 0.50±0.05 |
| Experimental group 3 | 12 pieces of | 38.4±4.0 | 0.54±0.05 |
| Experimental group 4 | 12 pieces of | 35.8±3.5 | 0.51±0.04 |
| Control group 1 | 12 pieces of | 21.7±2.5 | 0.32±0.05 |
| Control group 2 | 12 pieces of | 26.3±3.2 | 0.37±0.04 |
| Control group 3 | 12 pieces of | 28.5±3.8 | 0.40±0.04 |
| Blank control group | 12 pieces of | 24.3±2.5 | 0.35±0.04 |
Experimental example 7 Effect of the Chinese medicinal composition of the present invention on mouse lymphocyte transformation
ConA-induced splenic lymphocyte transformation experiments (MTT method) in mice: aseptically taking spleen, placing in a grinder containing appropriate amount of aseptic Hank's solution, gently grinding spleen to obtain cell suspension, and filtering with 200 mesh screen. Wash 2 times with Hank's solution, and centrifuge each time for 10min (1000 r/min). Then, the cells were suspended in 2mL of complete culture medium, the number of viable cells was counted, and the cell concentration was adjusted to 3X 105One per ml. The cell suspension was then added to a 24-well plate in two wells, 1ml per well, and 75. mu.l ConA solution (equivalent to 7.5. mu.g/ml) was added to one well, and 5% CO was added to the other well as a control2，37℃CO2Culturing for 72h in an incubator. 4 hours before the end of the culture, 0.7ml of the supernatant was gently aspirated from each well, and 0.7ml of calf serum-free RPMI 1640 culture medium was added thereto together with 50. mu.l/well of MTT (5mg/ml), and the culture was continued for 4 hours. After the culture is finished, 1ml of acidic isopropanol is added into each hole, and the mixture is uniformly blown and beaten to ensure that the purple crystals are completely dissolved. The optical density values were measured at 570nm with a semi-automatic biochemical analyzer. The proliferative capacity of lymphocytes is expressed as the optical density value of ConA plus wells minus the optical density value of ConA minus wells.
The results show that the proliferation capacity of the mouse lymphocytes induced by the ConA in the control group is slightly better than that of the blank control group, while the proliferation capacity of the mouse lymphocytes induced by the ConA in the experimental group is obviously better than that of the control group and the blank control group, and the traditional Chinese medicine composition has a remarkable enhancement effect on the transformation of the mouse lymphocytes induced by the ConA, and is detailed in the following table 9.
TABLE 9 Effect of the Chinese medicinal composition of the present invention on ConA-induced splenic lymphocyte transformation in mice
Experimental example 8 Effect of the Chinese medicinal composition of the present invention on NK cell Activity of mice
Determination of NK cell Activity: aseptically taking spleen, placing in a grinder containing appropriate amount of aseptic Hank's solution, gently grinding spleen to obtain cell suspension, filtering with 200 mesh screen, washing with Hank's solution for 2 times, centrifuging for 10min (1000r/min) each time, and adjusting cell concentration to 2 × 10 with RPMI 1640 complete culture solution7Taking 100 mul of target cells and effector cells respectively (the effective target ratio is 50:1), and adding the target cells and the effector cells into a U-shaped 96-hole culture plate; target cells are added into a natural release hole of the target cells and 100 mul of culture solution respectively, and target cells and 1% NP40 are added into a maximum release hole of the target cells and 100 mul of culture solution respectively; all the above-mentioned materials are equipped with three composite holes, at 37 deg.C and 5% CO2Culturing for 4h in an incubator, centrifuging the 96-well culture plate at 1500r/min for 5min, sucking 100 μ l of supernatant per well, placing the plate in a flat bottom 96-well culture plate, adding 100 μ l of LDH matrix solution, reacting for 3min, adding 30 μ l of 1mol/l hydrochloric acid per well, and measuring Optical Density (OD) at 490nm of a microplate reader.
NK cell activity% ([ (reaction pore OD-natural release pore OD)/(maximum release pore OD-natural release pore OD) ] × 100%
The results show that the NK cell activity of the experimental group mice is obviously higher than that of the control group and the blank control group, and the traditional Chinese medicine composition has a remarkable promoting effect on the NK cell activity of the mice, and is detailed in the following table 10.
TABLE 10 Effect of the Chinese medicinal composition of the present invention on NK cell Activity in mice
| Group of | Number of mice | NK cell Activity% |
| Experimental group 1 | 12 pieces of | 51.2±5.8 |
| Experimental group 2 | 12 pieces of | 49.5±4.3 |
| Experimental group 3 | 12 pieces of | 52.5±4.6 |
| Experimental group 4 | 12 pieces of | 52.7±4.0 |
| Control group 1 | 12 pieces of | 32.2±4.2 |
| Control group 2 | 12 pieces of | 35.5±5.3 |
| Control group 3 | 12 pieces of | 37.5±5.7 |
| Blank control group | 12 pieces of | 21.4±2.6 |
Claims (10)
1. A traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight:
3-5 parts of lucid ganoderma, wherein the lucid ganoderma is a natural ganoderma,
3-5 parts of wolfberry fruit,
3-5 parts of eucommia bark,
1-2 parts of ginseng (Panax ginseng C.A.Meyer),
0.5-1 part of cervus elaphus linnaeus.
2. The Chinese medicinal composition according to claim 1, wherein the ganoderma lucidum is a monarch drug, the medlar and the eucommia ulmoides are ministerial drugs, and the ginseng and the cervus elaphus linnaeus are assistant and guide drugs.
3. The Chinese medicinal composition according to claim 1, wherein the cervus elaphus linnaeus is cervus elaphus linnaeus powder.
4. A method for preparing a Chinese medicinal composition according to any one of claims 1 to 3, comprising the steps of:
1) decocting Ganoderma, fructus Lycii and Eucommiae cortex in water, filtering decoction, concentrating the filtrate, drying, and pulverizing into mixed powder;
2) drying Ginseng radix, and pulverizing into Ginseng radix powder, or directly weighing Ginseng radix powder;
3) drying cornu Cervi Pantotrichum, and pulverizing into powder, or directly weighing cornu Cervi Pantotrichum powder;
4) and (3) uniformly mixing the mixed powder, the ginseng powder and the cervus elaphus linnaeus powder to obtain the traditional Chinese medicine composition.
5. The method for preparing a Chinese medicinal composition according to claim 4, wherein the mixed powder, ginseng powder, and hairy antler powder are all passed through a five-mesh sieve, and contain not less than 95% of powder passed through a six-mesh sieve.
6. The method for preparing a Chinese medicinal composition according to claim 4, wherein the Ganoderma lucidum, Lycium barbarum fruit and eucommia ulmoides in step 1) are decocted in water for at least 2 times.
7. A Chinese medicinal preparation, which is prepared from the Chinese medicinal composition of any one of claims 1-3.
8. The Chinese medicinal preparation according to claim 7, which is a capsule, granule, tablet, pill, powder or oral liquid.
9. The traditional Chinese medicine preparation of claim 8, wherein the content of the capsule is 0.35 g/granule.
10. Use of the Chinese medicinal composition of any one of claims 1 to 3 or the Chinese medicinal preparation of any one of claims 7 to 9 for preparing a product with an effect of improving immunity.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115700276A (en) * | 2022-11-25 | 2023-02-07 | 广西农科环保科技有限公司 | Formula and preparation method of black soldier fly and ginseng nutritional wine |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106031418A (en) * | 2015-03-17 | 2016-10-19 | 德江洋山河生物科技有限公司 | Composition having physical power fatigue releasing function, and preparation method thereof |
| CN107647395A (en) * | 2017-10-13 | 2018-02-02 | 延边韩工坊健康制品有限公司 | It is a kind of that there is health food for improving immunity function and preparation method thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106031418A (en) * | 2015-03-17 | 2016-10-19 | 德江洋山河生物科技有限公司 | Composition having physical power fatigue releasing function, and preparation method thereof |
| CN107647395A (en) * | 2017-10-13 | 2018-02-02 | 延边韩工坊健康制品有限公司 | It is a kind of that there is health food for improving immunity function and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115700276A (en) * | 2022-11-25 | 2023-02-07 | 广西农科环保科技有限公司 | Formula and preparation method of black soldier fly and ginseng nutritional wine |
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