CN107647395A - It is a kind of that there is health food for improving immunity function and preparation method thereof - Google Patents
It is a kind of that there is health food for improving immunity function and preparation method thereof Download PDFInfo
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- CN107647395A CN107647395A CN201710949722.5A CN201710949722A CN107647395A CN 107647395 A CN107647395 A CN 107647395A CN 201710949722 A CN201710949722 A CN 201710949722A CN 107647395 A CN107647395 A CN 107647395A
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- health food
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- eucommia
- ganoderma lucidum
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- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 230000002969 morbid Effects 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229940092253 ovalbumin Drugs 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 229930015704 phenylpropanoid Natural products 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 239000009212 ren-shen-yang-rong-tang Substances 0.000 description 1
- 210000002955 secretory cell Anatomy 0.000 description 1
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- 230000008023 solidification Effects 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
- 210000000955 splenic vein Anatomy 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a kind of health food for having and improving immunity function, it is made up by weight ratio of the bark of eucommia 300 40, ganoderma lucidum 260 420, the fruit of Chinese wolfberry 260 420, peacilomyce hepiahi bacterium powder 100 250, ginseng 80 220.The present invention have strengthen the body resistance to consolidate the constitution, replenish qi to invigorate the spleen, the health food of nourishing liver and kidney effect, have fully regulation zang-fu organs qi movement, the health-care efficacy of strengthen immunity, contribute to strengthen body immunity, so as to improve the health status of hypoimmunity crowd.This product uses capsule simultaneously, had not only masked the poor taste of Chinese medicine but also had been easy to carry, and preferably can have been received by consumer.Food security of the present invention is effective.The invention also discloses a kind of preparation method for the health food for having and improving immunity function.
Description
Technical field
The invention belongs to technical field of health care food, in particular to it is a kind of have improve immunity function health food and
Its preparation method.
Background technology
Immunology Today thinks, immune to be body identification and remove non-self macromolecular complex, and keeping body internal and external equilibrium
Physiological reaction, i.e. identification of the body to " non-oneself " material, exclusion, a kind of process eliminated.The immunologic function of human body is both one
Road defence line, escorts health, is that one kind gets rid of illness ability again, can promote rehabilitation after people is ill, be that human body salubrity is disease-resistant
Basic place.However, in daily life, there are many factors to make human body immunity degradation or immunologic derangement occur.Such as meals
Eat unreasonable, protein, vitamin, micro- insufficiency of intake；Live irregular, have not enough sleep；Lack exercise exercise, to ring
The adaptability in border is poor；Stress, it is overworked；Use hormone, the adverse reaction of antibiotic etc, it is suppressed that immune
Power；Immunity is temporarily reduced when suffering from acute disease, hypoimmunity state etc. is then chronically at when suffering from chronic diseases.If for a long time
Hypoimmunity, intermediateness-sub-health state of health and disease just occurs in human body, and sub-health state is constantly to become
Change development, can not such as be improved in time, it is more likely that converted to morbid state.So human autoimmune's function is improved,
It is the basic method that health is moved towards away from inferior health.The related survey data in China shows that the crowd for having more than 60% is in Asia
Health status, it has been difficult to return to normal healthy state to rely solely on medicine symptomatic treatment.And the center of gravity of modern medicine turns to
Effective strick precaution of intervention and disease to state before disease, traditional Chinese medicine show unique characteristics in the adjustment to body allomeric function state, with
Chinese medicine is that raw material has the irreplaceable advantage of the health food for improving immunity function.It is effective to improve immunity health food
Increasingly it is valued by people and welcomes.
The content of the invention
It is an object of the invention to provide one kind to adjust function of human body, reaches having for strengthen immunity effect and improves immunity
The health food of function.
It is a further object to provide a kind of preparation method for the health food for having and improving immunity function.
The technical scheme is that：
It is a kind of that there is the health food for improving immunity function, it is made up by weight ratio of following raw materials according：
Bark of eucommia 300-400 ganoderma lucidum 260-420 fruits of Chinese wolfberry 260-420
Peacilomyce hepiahi bacterium powder 100-250 ginsengs 80-220.
Further, preferable described health food is made up by weight ratio of following raw materials according：
Bark of eucommia 320-380 ganoderma lucidum 280-400 fruits of Chinese wolfberry 280-400
Peacilomyce hepiahi bacterium powder 120-220 ginsengs 100-200.
Further, preferable described health food is made up by weight ratio of following raw materials according：
Bark of eucommia 340-360 ganoderma lucidum 300-380 fruits of Chinese wolfberry 300-380
Peacilomyce hepiahi bacterium powder 150-200 ginsengs 120-180.
4 is further, and preferable described health food is made up by weight ratio of following raw materials according：
The fruit of Chinese wolfberry 340 of 360 ganoderma lucidum of the bark of eucommia 340
The ginseng 150 of peacilomyce hepiahi bacterium powder 180.
Further, capsule form is made in described health food.
It is a kind of that there is the preparation method for improving immunity function health food as described above, comprise the following steps：
1）The bark of eucommia, ganoderma lucidum, the fruit of Chinese wolfberry, ginseng through net system, after the assay was approved, it is standby；
2）Peacilomyce hepiahi bacterium powder is standby through after the assay was approved；
3）Ginseng is taken to be extracted 1 time with 95%, 70%, 50%, 30% alcohol reflux respectively, each 5-10 times to measure alcohol reflux 1.5-3 small
When, merge extract solution, extract solution is filtered with 200 eye mesh screens, and merging filtrate, the dregs of a decoction are standby, filtrate recycling ethanol, and are concentrated into phase
It is standby to the medicinal extract that density is 1.30~1.35；
4）It is another take the bark of eucommia, ganoderma lucidum, the fruit of Chinese wolfberry and ginseng alcohol extracting after the dregs of a decoction add water to cook 2-3 time, every time plus 5-10 times is measured water, each pan-fried
Boil 2-3 hours, decocting liquid filtration, merging filtrate is simultaneously concentrated under reduced pressure into the medicinal extract that relative density is 1.30~1.35, standby；
5）Merge above two medicinal extract, be dried under reduced pressure, gained dry extract is broken into fine powder, crosses 80 mesh sieves, standby；
6）Above-mentioned fine powder and peacilomyce hepiahi bacterium powder are mixed, with 85% ethanol softwood, the granulation of 16 mesh sieves, 55~60 DEG C dry
It is dry, 14 mesh sieve whole grains, load No. 0 capsule, polish, pack, examine, get product.
In the present invention, the bark of eucommia is the dry bark of the Eucommiaceae plant bark of eucommia.It is sweet, it is warm-natured, return liver and kidney channel, have filling liver kidney,
Strengthening the bones and muscles, beneficial waist and knee, except aching the effect of." bark of eucommia, energy enter liver kidney tonifying, bowl spares strengthening the essence gas, hard muscles and bones, Qiang Zhi, controls kidney deficiency waist
Bitterly, long term usage, make light of one's life by commiting suicide resistance to old ".Research shows that the bark of eucommia is mainly containing lignanoids, Phenylpropanoid Glycosides class, iridoids, polysaccharide, flavones
Class, amino acid, gutta-percha, electrolytes and minerals etc..Bark of eucommia decocting liquid energy activated mononuclear-macrophage system and abdominal cavity
The phagocytic activity of macrophage system, strengthen the non-specific immune function of body.Cortex eucommiae total polyoses can preferably strengthen small
Mouse immunologic function.Eucommia bark polycose can excited reticuloendothelial system, enhancing body non-specific immune function, lignin and cyclenes
Ether ter penoidses have anticomplement binding activity.Experimental result shows that it is thin that bark of eucommia decocting liquid can dramatically increase mouse monokaryon macrophage
The phagocyte imdex K and phagocytic index of born of the same parents, and extend drop-out time of the mouse on tired instrument, improve mouse non-specificity and exempt from
Epidemic disease function.The bark of eucommia is remarkably reinforced effect to the phagocytic activity of Turnover of Mouse Peritoneal Macrophages.Bark of eucommia taste body tea can substantially increase small
The swelling degree of the paw of mouse, half hemolytic value, hence it is evident that strengthen the ability and NK of the peritoneal macrophage phagocytosis chicken red blood cells of mouse
Cytoactive, prompting the bark of eucommia to grow body tea has the function of strengthen immunity.It is red thin with sheep that the bark of eucommia can increase macaque lymphocyte
The combination of born of the same parents, prompt the bark of eucommia is immune to cynomolgus cells there is enhancing.
Ganoderma lucidum, it is the drying fructification of On Polyporaceae red sesame, its is sweet, mild-natured, the thoughts of returning home, lung, liver and kidney channel, has
The effect of invigorating qi for tranquilization, it is clinically used for consumptive disease and loses heart, cardio palmus of having a sleepless night.Ganoderma lucidum " strengthening by means of tonics ", " promoting longevity ", long term usage
Make one " good color ".The main active of ganoderma lucidum is GL-B, ganoderic acid, adenosine etc., and there is raising body cell to exempt from for it
The ability of epidemic disease and humoral immunity, the hyperplasia of lymphocyte can be promoted, strengthen the phagocytic activity of macrophage, improved mouse and kill naturally
Hinder cell（NK cells）Activity, improve state of activation under macrophage produce il-1（IL-1）White be situated between is produced with liver cell
Element -2（IL-2）Ability, can promote macrophage produce TNF（TNF）, improve hemolysin amount etc..Research shows,
Significantly effectively the delayed allergy of enhancing mouse is immune, antibody tormation is immune, monokaryon-macrophage for ganoderma lucidum decocting liquid energy
Immunocompetence and NK cytoactives, promote mice serum hemolysin to produce, illustrate that ganoderma lucidum can be from cellular immune function, body fluid
The many aspects such as immunologic function, monokaryon-macrophage function, NK cytoactives reach the work of enhancing mouse immune system function
With so as to play its strengthening by means of tonics, this effect of is returned in righting.Separately there is studies have shown that Ganoderma Polysaccharide to mouse S180Knurl has
Obvious inhibitory action, the mice spleen and thymus index that can resist caused by cyclophosphamide are reduced, hence it is evident that increase immune system gulps down
Function and Spleen cell proliferation number are bitten, can substantially increase the killing activity of NK cells, illustrates that Ganoderma Polysaccharide has and suppresses small
Mouse S180Knurl grows, and strengthens the effect of immunologic function.Research finds that GL-B to 2 weeks after mouse medicine feed, can strengthen mouse peritoneal
The phagocytic function of macrophage, promote the mouse lymphocyte multiplication capacity of ConA inductions, promote mouse reticuloendothelial system
Function, accelerate the removing of carbon granules, significantly improve NKT (NK) cell anti-tumor activity, the non-specific cell for strengthening body is exempted from
Epidemic disease function, dinitrofluorobenzene induced mice delayed allergy can be also significantly increased, show GL-B to body specificity
Cellular immune function also significantly increases effect.Its influence to mouse multiple immune indexes is observed using traditional ganoderma lucidum water decoction
It was found that the tool confrontation caused by cyclophosphamide murine interleukin quantity reduction effect of ganoderma lucidum decocting liquid, can increase immune organ weight, and
Macrophage phagocytic function can be strengthened, illustrate that it has humidification to nonspecific immunity of mice；In addition ganoderma lucidum decocting
Liquid energy significantly improves mouse peripheral blood T lymphocyte lipase rate of dyeing, illustrates that it can promote lymphocyte to mature T lymphocyte
Conversion, so as to improve mouse cell immunologic function；Other ganoderma lucidum decocting liquid energy substantially increases the content of hemolysin in mice serum,
Show that it has the function of stimulating B cell differentiation and proliferation to be changed into thick liquid cell, improve antibody level and exempt from so as to strengthen mouse body fluid
Epidemic disease function.
The fruit of Chinese wolfberry, it is the dry mature fruit of plant of Solanaceae lycium barbarum, is the Chinese medicine that tradition is conventional simply, kidney tonifying, profit
Lung, production of sperm, QI invigorating.The fruit of Chinese wolfberry is mild-natured, sweet, has the effect of nourishing liver and kidney, benefiting shrewd head.Contain multiple nutritional components in matrimony vine
It is one of main active of matrimony vine and trace element, wherein LBP-X have immunologic enhancement.Research finds, Chinese holly
Qi polysaccharide dosage group mouse thymus index, index and spleen index, B lymphocyte proliferation ability are all remarkably higher than inferior health model group, can
Improve the immunologic function of inferior health model mice.In addition, LBP-X have to the macrophage in difference in functionality state it is bright
Aobvious facilitation, and there is dual regulation, total T cell and TH subgroup percentages can be increased, improve lymphocyte transformation
Rate, human peripheral blood mononuclear cell, interleukin-22 and tumor necrosis factor alpha gene expression are improved, can also strengthen mouse and TD is resisted
The antibody response of primary stimuli, strengthen hemolysis plaque number and strengthen macrophage and NK cytoactives, hence it is evident that promote ConA activation
Splenic lymphocytes DNA and protein biosynthesis.Found by studying, each concentration group of Lycium Barbarum is to mouse body fluid and cell
Immunologic function is respectively provided with the effect of being obviously promoted, and can increase spleen index, thymus index, lymphocyte transformation rate and hemoglobin content
Add.Separately there are some researches show to mouse gavaging LBP-X, can make the increase of normal mouse peripheral leukocytes, improve immunosupress
The killing activity of NK cells in mice.Immunocompetence research to matrimony vine Thick many candies finds that matrimony vine Thick many candies increase to mouse boosting cell
Growing reaction and antibody formation reaction has obvious facilitation, and it is thin can substantially to increase Senescence-Accelerated Mouse spleen antibody tormation
The number of born of the same parents, it is horizontal that rise splenocyte produces IgG antibody.The experimental studies results of fruit of Chinese wolfberry enhancing immunization are shown, Chinese holly
Matrimony vine can substantially increase macrophage phagocytic function, make the hemolysis plaque showed increased of mouse boosting cell, the leaching for inducing ConA
Bar cell propagation, NK cytoactives dramatically increase.Experimental result shows that medlar hydro-apozem can resist ovalbumin in mice serum
Body and IL-2 growing amounts are significantly raised, illustrate that the fruit of Chinese wolfberry has immunoregulation effect.LBP-X can also make exempting from for normal mouse
Epidemic disease organ weight increases, and adjustment effect is played to the immunologic function of mouse.
Peacilomyce hepiahi bacterium powder, this product are Clavicipitaceae fungi bat moths isolated in fresh cordyceps sinensis sample
Paecilomyces varioti bacterial strain Cs-4 bacterial strains, through mycelial dried powder obtained by liquid deep layer fermenting.Peacilomyce hepiahi bacterium powder is country
The raw material available for health food of food and medicine Surveillance Authority accreditation, it is the artificial substituting product of cordyceps sinensis.Produced with Qinghai
High-quality natural cordyceps control, carrying out composition comparative analysis result proves：This product and cordyceps sinensis chemical composition are basically identical,
Pharmacological action is also essentially identical with cordyceps sinensis, and they are containing ucleosides, Cordyceps sinensis polysaccharide, cordycepic acid, ergosterol and a variety of
Amino acid, vitamin and trace element.Modern pharmacological research confirms that cordyceps sinensis can dramatically increase containing for mice serum lysozyme
Amount, pole significantly increase the titre of sheep red blood cell (SRBC), can also improve the phagocytic function of macrophage, improve lymphocyte transformation rate
With natural rosette forming rate, and it can substantially increase the content of serum IgG.Separately there is researcher it is demonstrated experimentally that cordyceps sinensis
Mouse antibodies can be strengthened and form (B cell) activity, it is thick liquid cell to promote B cell differentiation, propagation；Medicine group has compared with control group
Significant difference (P<0.05), the special rosette forming cell (RFC) of cordyceps sinensis group（SRFC）Number apparently higher than control group, with
Control group, which is compared, significant differences (P<0.01), cordyceps sinensis can make the increase of mice serum IgG content, with control group phase
Than there is significant differences (P<0.01), the prompting such as orally available cordyceps sinensis of body's immunity descender is exempted from strengthening body
Epidemic disease function（Jiang Tao etc., 2008）.To the artificial medium extract of aweto mycelium（TCKS）Immunestimulatory effect enter
Research is gone, it is found that its mouse oral gives the antibody-producing ability that can strengthen the anti-sheep red blood cell (SRBC) of mouse after TCKS.Jinshuibao glue
Capsule is by fermentation cordyceps（Cs-4）Manufactured, clinic paecilomyces hepiall chen treatment patient simultaneously observes immune indexes before and after medication
Change, as a result taking rear patient IgG, IgA, IgM content increases, with there is significant difference (P before taking<0.05), show
Paecilomyces hepiall chen can lift human antibody's level, strengthen body immunity.The cordyceps sinensis of separation and Extraction from cultural mycelium
Polysaccharide can significantly increase mouse phagocyte imdex and phagocytic index, and the immunologic hypofunction of enhancing hydrocortisone induction is small
The phagocytic function of mouse mononuclear macrophage；In delayed allergy experiment, Cordyceps sinensis polysaccharide dosage group can significantly increase small
Mouse ear swelling degree, Cordyceps sinensis polysaccharide is prompted to strengthen the hypoimmunity mice Tardive allergy of endoxan induction；Cordyceps sinensis is more
Sugared dosage group can significantly improve mouse hemolysin level, prompt Cordyceps sinensis polysaccharide to strengthen endoxan induction immunologic hypofunction small
The specific humoral immunity function of mouse, above result of the test illustrate that artificial aweto polysaccharide is non-specific to immunologic hypofunction mouse
There is certain humidification with specific immune function.The immunization for the cordyceps mycelia to cordyceps sinensis and manually cultivated is entered
Row paired observation, it is found that both of which can significantly improve the phagocytic index and percentage of Turnover of Mouse Peritoneal Macrophages, accelerate mouse blood
Middle carbon granule removing speed, show that cordyceps sinensis and cordyceps mycelia are respectively provided with the effect of enhancing body's immunity.To aweto
Silk（For the fermented hypha of peacilomyce hepiahi bacterium）Immunologic function is studied, and result of the test shows Chinese caterpillar fungus bacterial filament one
Under the conditions of fixed administration, there is obvious immunologic enhancement to mouse antibodies secretory cell and specific antigen-binding cells.
Ginseng, for the dry root and rhizome of Araliaceae ginseng, its sweet, slight bitter, slightly warm in nature, returns spleen, lung, the heart, kidney
Through, have reinforce vital energy, veins takes off admittedly, reinforce the spleen to benefit the lung, promote the production of body fluid, the effect of calming the nerves, be clinically used for it is physically weak to be taken off, spleen eating less,
The diseases such as insomnia of palpitating with fear.Ginseng is one of China's special product valuable ingredient of traditional Chinese medicine, with " main tonifying five zang organs, soothe the nerves, determine soul, and stop palpitate with fear is bright
Mesh, happy intelligence development, long term usage are made light of one's life by commiting suicide macrobiosis ".Ginseng main chemical compositions have saponins, polysaccharide, polypeptide, sterol
And its glycosides, amino acids and vitamin etc..Ability is produced as index using phagocytic activity, NK activity, IL-2, it is more in ginseng to mouse
The change of the lower immunologic function of sugar effect is observed, the results showed that and panaxan organizes 3 indexs and is remarkably reinforced compared with control group,
Illustrate that panaxan can improve the immunologic function of body.Separately there are some researches show, panaxan to the specific immunity of body and non-
Specific immunity has obvious facilitation：Daily by various dose to mouse gavaging panaxan 1 time, successive administration 3 times,
24h carries out detection of phagocytic function after last dose, and as a result panaxan can dramatically increase the phagocytic index of macrophage；To cavy
In gavaging panaxan 50mg/kg in 7 days daily, 24h after last dose, blood examination is taken to survey complement, as a result panaxan can be notable
Increase serum complement and the content of serum IgG；After mouse injection panaxan, spleen and thymic weight significantly increase compared with control group
Add.It is found by experiment that, panaxan can significantly increase the phagocytic function of reticuloendothelial system and can increase complement in serum
Content, while can substantially increase antibody dilution, haemolysis cellulose content, IgG content and B cell percentage, illustrate that panaxan has
There is strengthen immunity.Influence of the ginsenoside to hypoimmunity mice immunologic function is observed by pharmacological testing, as a result
Display ginsenoside can not only improve the organ index, carbonic clearance index and phagocytic index of hypoimmunity mice, can also improve and exempt from
Epidemic disease low mice serum IgM, IgG are horizontal, promote hypoimmunity mice delayed allergy, illustrate ginsenoside to immune low
Lower mouse nospecific immunity, specific immune function have certain facilitation.Pharmacology test discovery, ginsenoside
Rg3The phagocytic activity of NK cells can be remarkably reinforced and can substantially increase mice serum haemolysis cellulose content and antibody-producting cell number
Amount, shows ginsenoside Rg3Body mouse liquid immunologic function, reinforced partly non-specific immune function can be remarkably reinforced.Observer
Join influences of the saponin(e Re to mice with spleen deficiency nospecific immunity and find that ginsenoside Re can significantly improve mouse Carbon clearance energy
Power, promote body endothelium network to clean up phagocytic function to foreign matter, mouse body weight growth rate can be significantly improved, thymus gland refers to
Number and spleen index, prompt ginsenoside Re that there is facilitation to the immunologic function of mice with spleen deficiency.People is applied using chemotherapy combined radiotherapy
Join oral liquid effect tumor-bearing mice, observe its influence to mouse cell immunologic function, as a result show that ginseng oral liquid can strengthen
Radiotherapy tumor-bearing mice splenic T lymphocyte transformation rate, strengthen NK cytoactives, illustrate that ginseng oral liquid can strengthen tumor-bearing mice and put
Cellular immune function after treatment.Experimental study shows that Renshen Yangrong Tang can improve immunologic hypofunction mouse CTL cell killings
Activity, T lymphocyte quantities are raised, IgG, which is produced, has certain Pasitive Regulation Effect of Genseng.
The heretofore described bark of eucommia, ganoderma lucidum, the fruit of Chinese wolfberry, peacilomyce hepiahi bacterium powder, ginseng are marketable material.
In summary, the fruit of Chinese wolfberry is nourishing liver and kidney in inventive formulation, benefiting essence-blood, there is the effect of kidney tonifying gas, and ginseng reinforces vital energy,
Veins takes off admittedly, reinforce the spleen to benefit the lung, promote the production of body fluid, both yang-tonifyings of a tonifying yin one, the tonifying primordial Qi of a replenishing vital essence and blood one, yang blood and qi giving young employees remedial-courses in general knowledge and vocational skills, the five internal organs six
Internal organs is all suitable；It is aided with ganoderma lucidum invigorating qi for tranquilization, bark of eucommia filling liver kidney, strengthening the bones and muscles, beneficial waist and knee, peacilomyce hepiahi bacterium powder kidney tonifying benefit lung is all
Medicine compatibility plays the effect of strengthening the body resistance to consolidate the constitution, replenish qi to invigorate the spleen, be nourishing liver and kidney altogether, makes qi and blood full while first the foundation of acquired constitution is unbeaten, so as to
Body function is adjusted, reaches the purpose of strengthen immunity.The present inventor is according to the market demand, and with reference to traditional Chinese and western medicine theory, development is opened
Have issued with strengthen the body resistance to consolidate the constitution, replenish qi to invigorate the spleen, the health food of nourishing liver and kidney effect, have fully regulation zang-fu organs qi movement, enhancing
The health-care efficacy of immunity, help to strengthen body immunity, so as to improve the health status of hypoimmunity crowd.Originally simultaneously
Product uses capsule, had not only masked the poor taste of Chinese medicine but also had been easy to carry, and preferably can be received by consumer.The present invention
Food security is effective.
Brief description of the drawings
Fig. 1 is the flow chart of preparation method of the present invention.
Embodiment
With reference to embodiment, the present invention is described in more detail.But skilled in the art realises that following embodiments
It is not limiting the scope of the invention, any improvement and change made on the basis of the present invention, all guarantor in the present invention
Within the scope of shield.
Embodiment 1
1）The parts by weight of the bark of eucommia 380, the parts by weight of ganoderma lucidum 400, the parts by weight of the fruit of Chinese wolfberry 400, the parts by weight of ginseng 200, through net system, examine and close
Used after lattice for preparation.
2）The parts by weight of peacilomyce hepiahi bacterium powder 220, through being used after the assay was approved for preparation.
3）The parts by weight of ginseng 200 are taken, is extracted 1 time with 95%, 70%, 50%, 30% alcohol reflux respectively, measures ethanol for 8 times every time
Backflow 1.5 hours, merge extract solution, extract solution is filtered with 200 eye mesh screens, merging filtrate（The dregs of a decoction are standby）, filtrate recycling ethanol
（- 0.065~-0.075Mpa, 65 DEG C）, and relative density is concentrated into as 1.30~1.35（60 DEG C of surveys）Medicinal extract, it is standby.
4）It is another take the parts by weight of the bark of eucommia 380, the parts by weight of ganoderma lucidum 400, the parts by weight of the fruit of Chinese wolfberry 400 and ginseng alcohol extracting after the dregs of a decoction add water
Decoct 3 times, every time plus 10 times are measured water, and each to decoct 2 hours, decocting liquid filtration, merging filtrate is simultaneously concentrated under reduced pressure（- 0.065~-
0.075Mpa, 75 DEG C）It is 1.30~1.35 to relative density（60℃）Medicinal extract, it is standby.
5）Merge above two medicinal extract, be dried under reduced pressure（- 0.085Mpa, 65 DEG C）, gained dry extract is broken into fine powder, mistake
80 mesh sieves, it is standby.
6）Above-mentioned fine powder and peacilomyce hepiahi bacterium powder are mixed, with 85% ethanol softwood, 16 mesh sieves are pelletized, and 55~60
DEG C drying, 14 mesh sieve whole grains, load No. 0 capsule, polish, pack, examine, get product.
Embodiment 2
1）The parts by weight of the bark of eucommia 320, the parts by weight of ganoderma lucidum 280, the parts by weight of the fruit of Chinese wolfberry 280, the parts by weight of ginseng 100, through net system, examine and close
Used after lattice for preparation.
2）The parts by weight of peacilomyce hepiahi bacterium powder 120, through being used after the assay was approved for preparation.
3）The parts by weight of ginseng 100 are taken, is extracted 1 time with 95%, 70%, 50%, 30% alcohol reflux respectively, measures ethanol for 5 times every time
Backflow 3 hours, merge extract solution, extract solution is filtered with 200 eye mesh screens, merging filtrate（The dregs of a decoction are standby）, filtrate recycling ethanol（-
0.065~-0.075Mpa, 65 DEG C）, and relative density is concentrated into as 1.30~1.35（60 DEG C of surveys）Medicinal extract, it is standby.
4）It is another take the parts by weight of the bark of eucommia 320, the parts by weight of ganoderma lucidum 280, the parts by weight of the fruit of Chinese wolfberry 280 and ginseng alcohol extracting after the dregs of a decoction add water
Decoct 3 times, every time plus 5 times are measured water, and each to decoct 3 hours, decocting liquid filtration, merging filtrate is simultaneously concentrated under reduced pressure（- 0.065~-
0.075Mpa, 75 DEG C）It is 1.30~1.35 to relative density（60℃）Medicinal extract, it is standby.
5）Merge above two medicinal extract, be dried under reduced pressure（- 0.085Mpa, 65 DEG C）, gained dry extract is broken into fine powder, mistake
80 mesh sieves, it is standby.
6）Above-mentioned fine powder and peacilomyce hepiahi bacterium powder are mixed, with 85% ethanol softwood, 16 mesh sieves are pelletized, and 55~60
DEG C drying, 14 mesh sieve whole grains, load No. 0 capsule, polish, pack, examine, get product.
Embodiment 3
1）The parts by weight of the bark of eucommia 360, the parts by weight of ganoderma lucidum 300, the parts by weight of the fruit of Chinese wolfberry 300, the parts by weight of ginseng 180, through net system, examine and close
Used after lattice for preparation.
2）The parts by weight of peacilomyce hepiahi bacterium powder 150, through being used after the assay was approved for preparation.
3）The parts by weight of ginseng 180 are taken, is extracted 1 time with 95%, 70%, 50%, 30% alcohol reflux respectively, measures ethanol for 6 times every time
Backflow 2.5 hours, merge extract solution, extract solution is filtered with 200 eye mesh screens, merging filtrate（The dregs of a decoction are standby）, filtrate recycling ethanol
（- 0.065~-0.075Mpa, 65 DEG C）, and relative density is concentrated into as 1.30~1.35（60 DEG C of surveys）Medicinal extract, it is standby.
4）It is another take the parts by weight of the bark of eucommia 360, the parts by weight of ganoderma lucidum 300, the parts by weight of the fruit of Chinese wolfberry 300 and ginseng alcohol extracting after the dregs of a decoction add water
Decoct 3 times, every time plus 8 times are measured water, and each to decoct 2.5 hours, decocting liquid filtration, merging filtrate is simultaneously concentrated under reduced pressure（- 0.065~-
0.075Mpa, 75 DEG C）It is 1.30~1.35 to relative density（60℃）Medicinal extract, it is standby.
5）Merge above two medicinal extract, be dried under reduced pressure（- 0.085Mpa, 65 DEG C）, gained dry extract is broken into fine powder, mistake
80 mesh sieves, it is standby.
6）Above-mentioned fine powder and peacilomyce hepiahi bacterium powder are mixed, with 85% ethanol softwood, 16 mesh sieves are pelletized, and 55~60
DEG C drying, 14 mesh sieve whole grains, load No. 0 capsule, polish, pack, examine, get product.
Embodiment 4
1）The parts by weight of the bark of eucommia 360, the parts by weight of ganoderma lucidum 340, the parts by weight of the fruit of Chinese wolfberry 340, the parts by weight of ginseng 150, through net system, examine and close
Used after lattice for preparation.
2）The parts by weight of peacilomyce hepiahi bacterium powder 180, through being used after the assay was approved for preparation.
3）The parts by weight of ginseng 150 are taken, is extracted 1 time with 95%, 70%, 50%, 30% alcohol reflux respectively, measures ethanol for 7 times every time
Backflow 2 hours, merge extract solution, extract solution is filtered with 200 eye mesh screens, merging filtrate（The dregs of a decoction are standby）, filtrate recycling ethanol（-
0.065~-0.075Mpa, 65 DEG C）, and relative density is concentrated into as 1.30~1.35（60 DEG C of surveys）Medicinal extract, it is standby.
4）It is another take the parts by weight of the bark of eucommia 360, the parts by weight of ganoderma lucidum 340, the parts by weight of the fruit of Chinese wolfberry 340 and ginseng alcohol extracting after the dregs of a decoction add water
Decoct 2 times, every time plus 10 times are measured water, and each to decoct 2 hours, decocting liquid filtration, merging filtrate is simultaneously concentrated under reduced pressure（- 0.065~-
0.075Mpa, 75 DEG C）It is 1.30~1.35 to relative density（60℃）Medicinal extract, it is standby.
5）Merge above two medicinal extract, be dried under reduced pressure（- 0.085Mpa, 65 DEG C）, gained dry extract is broken into fine powder, mistake
80 mesh sieves, it is standby.
6）Above-mentioned fine powder and peacilomyce hepiahi bacterium powder are mixed, with 85% ethanol softwood, 16 mesh sieves are pelletized, and 55~60
DEG C drying, 14 mesh sieve whole grains, load No. 0 capsule, polish, pack, examine, get product.
Test example：
The beneficial effect of capsule of the present invention is expanded on further below by way of test example, these test examples include 5 immune groups, often
40 mouse are one group.It is immune I group, carry out the mouse spleen lymphocyte transformation experiment of ConA inductions, NK cytoactive detections；Exempt from
II group of epidemic disease, carry out delayed allergy experiment；It is immune III group, carry out dirty/body ratio measurement, serum hemolysin measure and antibody
Cellulation number determines；It is immune IV group, carry out carbonic clearance experiment；It is immune V group, carry out Turnover of Mouse Peritoneal Macrophages phagocytosis chicken blood
Red blood cell is tested.
Capsule crowd recommends daily intaking amount 2.4g/60kg BW（That is 40mg/kg BW）, expand 5,10,20 times, set
Basic, normal, high three dosage groups are respectively 200,400,800mg/kg BW, each group experimental animal number（SPF levels Kunming kind male is small
Mouse, 18-22g, 200）It is 10.Weigh 1.0 respectively, 2.0,4.0g capsule 's contents, respectively plus pure water is to 100mL, mixes,
Be made into 10,20, the suspension of 40mg/mL concentration, give corresponding dosage group animal gavage respectively, gavage volume is 0.2mL/10g
BW, negative control group give isometric pure water, daily gavage once, continuous gavage 30-35 days.
It is immune I group：The influence for the mouse spleen lymphocyte conversion capability that given the test agent is induced ConA, given the test agent is to small
The influence of the NK cytoactives of mouse.
The mouse spleen lymphocyte transformation experiment of ConA inductions（Mtt assay）
Method：It is sterile to take spleen, it is placed in and fills appropriate sterile Hank,In the plate of s liquid, cell suspension is made, through 200 eye mesh screen mistakes
Filter.Use Hank,S liquid is washed 2 times, centrifuges 10min every time（1000r/min）.Then cell is suspended in 1mL complete culture solutions,
Living cell counting number, it is 3 × 10 with RPMI1640 nutrient solutions adjustment cell concentration6Individual/mL.Cell suspension point holes is added again
In 24 well culture plates, per hole 1mL, a hole adds 75 μ L ConA liquid wherein（Equivalent to 7.5 μ g/mL）, another hole, which is used as, to be compareed,
Put 5% CO2, 72h is cultivated in 37 DEG C of carbon dioxide incubators.Culture terminates preceding 4h, gently sucks supernatant 0.7mL per hole, adds
0.7mL is free of the RPMI1640 nutrient solutions of calf serum, while adds MTT（5mg/mL）50 μ L/ holes, continue to cultivate 4h.Culture
After end, 1mL acid isopropyl alcohol is added per hole, piping and druming mixes, and is completely dissolved purple crystal.Then 96 well culture plates are dispensed into
In, 3 parallel holes are made in each hole, and with ELIASA, OD value is determined with 570nm wavelength.The competence for added value of lymphocyte, which is used, to be added
The OD value in ConA holes subtracts the OD value expression for being not added with ConA holes.
NK cytoactive detections（LDH determination methods）
Method：
Mouse cervical dislocation is put to death, it is sterile to take spleen, splenocyte suspension is made, uses Hank,S liquid is washed 2 times, centrifuges 10min every time
（1000r/min）.Supernatant is abandoned, cytoplasm is upspring, adds 0.5mL sterilizings pure water 20 seconds, 0.5mL is added after splitting erythrocyte
2 times of Hank,S liquid and 8mL Hank,S liquid, 1000r/min centrifugations 10min, the RPMI1640 that 10% calf serum is contained with 1mL are complete
Nutrient solution is resuspended, and is counted after being diluted with 1% glacial acetic acid（Viable count should be more than 95%）, with platform phenol orchid dyeing counting living cells
Number, it is finally 2 × 10 with RPMI1640 complete culture solutions adjustment cell concentration7Individual/mL, this is effector cell.Take 24h after passing on
Well-grown YAC-1 cells, it is 4 × 10 with RPMI1640 complete culture solutions adjustment cell concentration5Individual/mL, this is target cell.
Take target cell and each 100 μ L of effector cell（Target is imitated than 50:1）, add in U-shaped 96 well culture plate；Target cell Spontaneous release hole adds
Target cell and each 100 μ L of nutrient solution, target cell maximum release aperture add target cell and each 100 μ L of 1% NP40.Above-mentioned items respectively set 3
Individual parallel hole, put 5% CO2, cultivate 4h in 37 DEG C of carbon dioxide incubators.Then 96 well culture plates are centrifuged with 1500r/min
5min, drawn per hole in the well culture plate of 100 μ L horizontalizations bottom of supernatant 96, while add the μ L of LDH matrix liquids 100, react 8min, per hole
1mol/L HCL30 μ L are added, optical density is determined at ELIASA 490nm（OD）Value.Press column count NK cytoactives.
NK cytoactives（%）=（Reacting hole OD- Spontaneous releases hole OD）/（Maximum release aperture OD- Spontaneous releases hole OD）×
100%
Result of the test：
The Splenic vein hemodynamics ability of each dosage group mouse of given the test agent is above negative control group, and high dose group and feminine gender
The difference of control group has conspicuousness（P ＜ 0.05）, show that the sample has the spleen lymphocyte proliferation for promoting mouse, conversion energy
The effect of power, is shown in Table 1.
The NK cytoactives of each dosage group mouse of given the test agent are above negative control group, and high dose group and negative control
The difference of group has very significant（P ＜ 0.01）, show that the sample has the function that the NK cytoactives for promoting hour, be shown in Table
2。
It is immune II group：Given the test agent is to mouse delayed allergy（DTH）Influence
Method：Dinitrofluorobenzene inducing mouse DTH is tested（Ear swelling method）
Experiment terminate first 5 days with barium sulphide by mouse part skin lose hair or feathers about 3cm × 3cm scopes, it is uniform with 50 μ L DNFB solution
Sensitization is smeared, 10 μ L DNFB are uniformly applied to mouse right ear two sides after 5 days and attacked, cervical dislocation is put to death after 24 hours
Mouse, left and right auricular concha is cut, remove 8mm diameter auricles with card punch, weigh, DTH journey is represented with the difference of left and right ear weight
Degree.
Result of the test：The left and right auricle weight difference of each dosage group mouse of given the test agent is higher than negative control group, but each dose
There are no significant for the difference of amount group and negative control group（P ＞ 0.05）, show delayed allergy of the sample to mouse without
Obvious facilitation, is shown in Table 3.
It is immune III group：Influence of the given the test agent to mouse immune organ internal organs/body weight ratio, to mice serum hemolysin
Influence and the influence to mouse antibodies cellulation number.
Internal organs/body weight ratio measurement
Method：Mouse is put to death after weighing, thymus gland and spleen is taken out, is weighed on electronic analytical balance, calculates dirty/body ratio.
The measure of serum hemolysin（Hemagglutination Method）
Method：The sheep blood of de- fiber is taken, with brine 3 times, centrifuges 10min every time（2000r/min）, use physiological saline
Hematocrit SRBC is made into 2%（v/v）Cell suspension, per mouse intraperitoneal injection 0.2mL.After immune 5 days, the eyeball of mouse is extractd, is taken
Blood 2000r/min centrifugation 10min, separation, collects serum in centrifuge tube, placing about 1h.It is with physiological saline that serum multiple proportions is dilute
Release, the serum of different dilution factors is respectively placed in Microhemagglutination plate, per the μ L of hole 100, add 100 μ L 0.5%（v/v）'s
SRBC suspensions, mix, load in the square position of moistening and be capped, in 37 DEG C of incubation 3h, observe hemagglutination degree.Count as the following formula
Calculate antibody product：
Antibody product=（S1+2S2+3S3……nSn）
1,2,3 ... n are the index of two-fold dilution in formula, and S is the rank of aggegation degree.
Antibody-producting cell detection（Jerne improves slide methods）
Method：The sheep blood of de- fiber is taken, with brine 3 times, centrifuges 10min every time（2000r/min）, use physiological saline
Hematocrit SRBC is made into 2%（V/V）Cell suspension, per mouse intraperitoneal injection 0.2ml.The mouse of 5 days is put to death after will be immune, takes spleen
Dirty put fills Hank,In the plate of s liquid, spleen is gently ground, cell suspension is made, through 200 mesh sieve net filtrations, centrifuges 10min
（2000r/min）, use Hank,S liquid is washed 2 times, and cell finally is suspended in into 8mL Hank,In s liquid.By top layer culture medium（1g fine jades
Lipolysaccharide adds distilled water to 100mL）After heating for dissolving, 45-50 DEG C of water-bath insulation is put, with equivalent PH7.2-7.4,2 times of concentration
Hank,S liquid mixes, and dispenses small test tube, often pipe 0.5mL, then 50 μ L10%SRBC are added into pipe（V/v, matched somebody with somebody with SA buffer solutions
System）, 25 μ L splenocyte suspensions, it is rapid mix after be poured on the slide of brush agarose thin layer, do parallel plate, treat agar solidification
Afterwards, slide is put down to buckle and be placed in glass frame, be put into CO2gas incubator and be incubated 1.5h, the complement diluted with SA buffer solutions
（1:8）Add in glass frame groove, continue to be incubated 1.5h, count hemolysis plaque number.With plaque number/106Splenocyte represents antibody
Cellulation number.
Result of the test：
Thymus gland/body weight and spleen/body weight ratio of each dosage group of given the test agent are compared with negative control group, difference that there are no significant
（P ＞ 0.05）, show that the sample has no significant effect to the immune organ weight of mouse, be shown in Table 4.
The antibody product of each dosage mouse of given the test agent is higher than negative control group, and the difference of high dose group and negative control group
It is different that there is conspicuousness（P ＜ 0.05）, show that the sample has the function that the serum hemolysin for improving mouse, be shown in Table 5.
The hemolysis plaque number of each dosage group mouse of given the test agent is higher than negative control group, and high dose group, middle dose group with
The difference of negative control group has very significant（P ＜ 0.01）, show that the sample has the antibody-producting cell for promoting mouse
The effect of propagation, is shown in Table 6.
It is immune IV group：Influence of the sample to the monocytes/macrophages carbonic clearance of mouse
Method：India ink through tail vein to 4 times of mouse injection normal saline dilution, per 10g body weight injection 0.1mL, ink
Timing immediately after juice injection, the 2nd, 10min after prepared Chinese ink is injected, takes the μ L of blood 20 from angular vein clump respectively, is added to 2mL0.1%
Na2CO3In solution, shake up.With Na2CO3Solution does blank control, with 600nm wavelength densitometric values（OD）.Mouse is put to death,
Liver, spleen are taken, is weighed.Phagocytic index a is calculated as follows.
a=K1/3× body weight/（Liver weight+spleen weight）
K in formula=（lgOD1-lgOD2）/（t2-t1）
Result of the test：
The phagocytic index of each dosage mouse of given the test agent is higher than negative control group, and the difference of high dose group and negative control group has
There is conspicuousness（P ＜ 0.05）, show that the sample has the function that the monocytes/macrophages carbonic clearance function of promoting mouse, be shown in Table
7。
It is immune V group：Sample swallows the influence of chicken red blood cells ability to Turnover of Mouse Peritoneal Macrophages
Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell experiment（Half intracorporal method）
Method：To mouse peritoneal injection 20%（V/v, use normal saline）Chicken erythrocyte suspension, per mouse 1mL, interval
30min, cervical dislocation put to death mouse, and center cuts off abdominal skin, Intraperitoneal injection 2mL physiological saline, rotates mouse plate 1min, suctions out
Abdominal cavity washing lotion 1mL, mean droplet are put into the enamel box for being lined with wet gauze on 2 slides, are put 37 DEG C of incubators and are incubated 30min.
Incubate it is complete, take out slide rinsed in physiological saline after dry, use methanol：Acetone（1:1）Solution is fixed, and 4%（v/v）Giemsa- phosphorus
Acid buffer dyes, then is rinsed, dried with pure water.Under oil mirror every counting 100 macrophages, be calculated as follows phagocytic rate and
Phagocytic index.
Phagocytic rate（%）Number of macrophages × 100 of number of macrophages/counting of=phagocytosis chicken red blood cell
The number of macrophages of the chicken red blood cell sum/counting for phagocytic index=swallowed
Result of the test：
The peritoneal macrophage of each dosage mouse of given the test agent is above negative right to the phagocytic rate and phagocytic index of chicken red blood cell
According to group, and the difference of the phagocytic rate of high dose group and phagocytic index and negative control group has conspicuousness（P ＜ 0.05）, show this
Sample has the function that the Peritoneal Macrophage Phagocytosis for promoting mouse, is shown in Table 8.
Conclusion：Give given the test agent 30-35 days, spleen lymphocyte proliferation, the transformation of mouse can be promoted, promote mouse
Antibody-producting cell propagation, improve the serum hemolysin of mouse, promote the phagocytosis energy of the monocytes/macrophages of mouse
Power, promote the NK cytoactives of mouse, it is abnormal to thymus gland/body weight ratio of mouse, spleen/body weight ratio and the delayed of mouse
Reaction has no significant effect, and prompts the sample to have strengthen immunity function.
Claims (6)
1. a kind of have the health food for improving immunity function, it is made up by weight ratio of following raw materials according：
Bark of eucommia 300-400 ganoderma lucidum 260-420 fruits of Chinese wolfberry 260-420
Peacilomyce hepiahi bacterium powder 100-250 ginsengs 80-220.
A kind of 2. health food with raising immunity function according to claim 1, it is characterised in that described guarantor
Health food is made up by weight ratio of following raw materials according：
Bark of eucommia 320-380 ganoderma lucidum 280-400 fruits of Chinese wolfberry 280-400
Peacilomyce hepiahi bacterium powder 120-220 ginsengs 100-200.
A kind of 3. health food with raising immunity function according to claim 1, it is characterised in that described guarantor
Health food is made up by weight ratio of following raw materials according：
Bark of eucommia 340-360 ganoderma lucidum 300-380 fruits of Chinese wolfberry 300-380
Peacilomyce hepiahi bacterium powder 150-200 ginsengs 120-180.
A kind of 4. health food with raising immunity function according to claim 1, it is characterised in that described guarantor
Health food is made up by weight ratio of following raw materials according：
The fruit of Chinese wolfberry 340 of 360 ganoderma lucidum of the bark of eucommia 340
The ginseng 150 of peacilomyce hepiahi bacterium powder 180.
A kind of 5. health food with raising immunity function according to claim 1, it is characterised in that described guarantor
Capsule form is made in health food.
6. a kind of have the preparation method for improving immunity function health food as claimed in claim 1, comprise the following steps：
1）The bark of eucommia, ganoderma lucidum, the fruit of Chinese wolfberry, ginseng through net system, after the assay was approved, it is standby；
2）Peacilomyce hepiahi bacterium powder is standby through after the assay was approved；
3）Ginseng is taken to be extracted 1 time with 95%, 70%, 50%, 30% alcohol reflux respectively, each 5-10 times to measure alcohol reflux 1.5-3 small
When, merge extract solution, extract solution is filtered with 200 eye mesh screens, and merging filtrate, the dregs of a decoction are standby, filtrate recycling ethanol, and are concentrated into phase
It is standby to the medicinal extract that density is 1.30~1.35；
4）It is another take the bark of eucommia, ganoderma lucidum, the fruit of Chinese wolfberry and ginseng alcohol extracting after the dregs of a decoction add water to cook 2-3 time, every time plus 5-10 times is measured water, each pan-fried
Boil 2-3 hours, decocting liquid filtration, merging filtrate is simultaneously concentrated under reduced pressure into the medicinal extract that relative density is 1.30~1.35, standby；
5）Merge above two medicinal extract, be dried under reduced pressure, gained dry extract is broken into fine powder, crosses 80 mesh sieves, standby；
6）Above-mentioned fine powder and peacilomyce hepiahi bacterium powder are mixed, with 85% ethanol softwood, the granulation of 16 mesh sieves, 55~60 DEG C dry
It is dry, 14 mesh sieve whole grains, load No. 0 capsule, polish, pack, examine, get product.
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| CN110179115A (en) * | 2019-07-12 | 2019-08-30 | 早康枸杞股份有限公司 | A kind of fructus lycii Chinese medicine health-care magma with strengthen immunity |
| CN111820415A (en) * | 2020-09-02 | 2020-10-27 | 延寿县鼎鑫生物工程有限公司 | Hard capsule with immunity enhancing and health care functions and preparation method thereof |
| CN112494569A (en) * | 2020-12-30 | 2021-03-16 | 森隆药业有限公司 | Traditional Chinese medicine composition for improving immunity and preparation method and application thereof |
| CN113564212A (en) * | 2021-07-26 | 2021-10-29 | 浙江树人学院（浙江树人大学） | Method for extracting eucommia ulmoides leaf polysaccharide by using microbial fermentation method |
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