CN105360824A - Chinese caterpillar fungus beverage and preparation method thereof - Google Patents
Chinese caterpillar fungus beverage and preparation method thereof Download PDFInfo
- Publication number
- CN105360824A CN105360824A CN201510776271.0A CN201510776271A CN105360824A CN 105360824 A CN105360824 A CN 105360824A CN 201510776271 A CN201510776271 A CN 201510776271A CN 105360824 A CN105360824 A CN 105360824A
- Authority
- CN
- China
- Prior art keywords
- water
- inspissated juice
- sweetener
- fruit
- date
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002360 preparation method Methods 0.000 title claims description 15
- 235000013361 beverage Nutrition 0.000 title abstract description 10
- 241000233866 Fungi Species 0.000 title abstract description 7
- 240000001307 Myosotis scorpioides Species 0.000 title abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 83
- 241001264174 Cordyceps militaris Species 0.000 claims abstract description 36
- 240000001008 Dimocarpus longan Species 0.000 claims abstract description 36
- 235000000235 Euphoria longan Nutrition 0.000 claims abstract description 35
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 35
- 244000241872 Lycium chinense Species 0.000 claims abstract description 32
- 235000015468 Lycium chinense Nutrition 0.000 claims abstract description 32
- 235000003599 food sweetener Nutrition 0.000 claims abstract description 23
- 239000003765 sweetening agent Substances 0.000 claims abstract description 23
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 claims abstract description 7
- 230000036039 immunity Effects 0.000 claims abstract description 7
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 66
- 239000000284 extract Substances 0.000 claims description 53
- 239000007788 liquid Substances 0.000 claims description 42
- 238000011049 filling Methods 0.000 claims description 40
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 35
- 238000009835 boiling Methods 0.000 claims description 30
- 239000000463 material Substances 0.000 claims description 29
- 239000000203 mixture Substances 0.000 claims description 20
- 241000190633 Cordyceps Species 0.000 claims description 17
- 238000003556 assay Methods 0.000 claims description 17
- 238000000605 extraction Methods 0.000 claims description 17
- 239000012809 cooling fluid Substances 0.000 claims description 16
- 239000008236 heating water Substances 0.000 claims description 16
- 239000006166 lysate Substances 0.000 claims description 16
- 230000001954 sterilising effect Effects 0.000 claims description 14
- 238000004659 sterilization and disinfection Methods 0.000 claims description 14
- 238000004853 microextraction Methods 0.000 claims description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 10
- 229930006000 Sucrose Natural products 0.000 claims description 10
- 235000021552 granulated sugar Nutrition 0.000 claims description 10
- 239000002994 raw material Substances 0.000 claims description 9
- 241000202807 Glycyrrhiza Species 0.000 claims description 6
- 235000001453 Glycyrrhiza echinata Nutrition 0.000 claims description 6
- 235000017382 Glycyrrhiza lepidota Nutrition 0.000 claims description 6
- 229940010454 licorice Drugs 0.000 claims description 6
- -1 Radix Glycyrrhizae Chemical compound 0.000 claims description 5
- 235000009508 confectionery Nutrition 0.000 claims description 5
- 235000013305 food Nutrition 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- 230000036541 health Effects 0.000 claims description 4
- 235000012907 honey Nutrition 0.000 claims description 4
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- FTLYMKDSHNWQKD-UHFFFAOYSA-N (2,4,5-trichlorophenyl)boronic acid Chemical compound OB(O)C1=CC(Cl)=C(Cl)C=C1Cl FTLYMKDSHNWQKD-UHFFFAOYSA-N 0.000 claims description 2
- NUFKRGBSZPCGQB-FLBSXDLDSA-N (3s)-3-amino-4-oxo-4-[[(2r)-1-oxo-1-[(2,2,4,4-tetramethylthietan-3-yl)amino]propan-2-yl]amino]butanoic acid;pentahydrate Chemical compound O.O.O.O.O.OC(=O)C[C@H](N)C(=O)N[C@H](C)C(=O)NC1C(C)(C)SC1(C)C.OC(=O)C[C@H](N)C(=O)N[C@H](C)C(=O)NC1C(C)(C)SC1(C)C NUFKRGBSZPCGQB-FLBSXDLDSA-N 0.000 claims description 2
- 239000004377 Alitame Substances 0.000 claims description 2
- 108010011485 Aspartame Proteins 0.000 claims description 2
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 claims description 2
- 239000004383 Steviol glycoside Substances 0.000 claims description 2
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 2
- 235000019409 alitame Nutrition 0.000 claims description 2
- 108010009985 alitame Proteins 0.000 claims description 2
- 239000000605 aspartame Substances 0.000 claims description 2
- 235000010357 aspartame Nutrition 0.000 claims description 2
- IAOZJIPTCAWIRG-QWRGUYRKSA-N aspartame Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(=O)OC)CC1=CC=CC=C1 IAOZJIPTCAWIRG-QWRGUYRKSA-N 0.000 claims description 2
- 229960003438 aspartame Drugs 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 claims description 2
- 235000019534 high fructose corn syrup Nutrition 0.000 claims description 2
- 235000014666 liquid concentrate Nutrition 0.000 claims description 2
- 239000000845 maltitol Substances 0.000 claims description 2
- 235000010449 maltitol Nutrition 0.000 claims description 2
- VQHSOMBJVWLPSR-WUJBLJFYSA-N maltitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O VQHSOMBJVWLPSR-WUJBLJFYSA-N 0.000 claims description 2
- 229940035436 maltitol Drugs 0.000 claims description 2
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 2
- 238000001223 reverse osmosis Methods 0.000 claims description 2
- 229940085605 saccharin sodium Drugs 0.000 claims description 2
- 235000019411 steviol glycoside Nutrition 0.000 claims description 2
- 229930182488 steviol glycoside Natural products 0.000 claims description 2
- 150000008144 steviol glycosides Chemical class 0.000 claims description 2
- 235000019202 steviosides Nutrition 0.000 claims description 2
- 150000005846 sugar alcohols Chemical class 0.000 claims description 2
- 239000000811 xylitol Substances 0.000 claims description 2
- 235000010447 xylitol Nutrition 0.000 claims description 2
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims description 2
- 229960002675 xylitol Drugs 0.000 claims description 2
- 229930195725 Mannitol Natural products 0.000 claims 1
- 239000000594 mannitol Substances 0.000 claims 1
- 235000010355 mannitol Nutrition 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 21
- 241000756042 Polygonatum Species 0.000 abstract description 2
- 244000303040 Glycyrrhiza glabra Species 0.000 abstract 1
- 235000017443 Hedysarum boreale Nutrition 0.000 abstract 1
- 235000007858 Hedysarum occidentale Nutrition 0.000 abstract 1
- 235000006545 Ziziphus mauritiana Nutrition 0.000 abstract 1
- 244000126002 Ziziphus vulgaris Species 0.000 abstract 1
- 235000008529 Ziziphus vulgaris Nutrition 0.000 abstract 1
- 239000001947 glycyrrhiza glabra rhizome/root Substances 0.000 abstract 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 37
- 240000006698 Spigelia anthelmia Species 0.000 description 35
- 241001465754 Metazoa Species 0.000 description 32
- 238000012360 testing method Methods 0.000 description 29
- 210000004027 cell Anatomy 0.000 description 23
- 210000000952 spleen Anatomy 0.000 description 22
- 210000003743 erythrocyte Anatomy 0.000 description 17
- 239000000047 product Substances 0.000 description 16
- 230000000242 pagocytic effect Effects 0.000 description 15
- 230000006870 function Effects 0.000 description 14
- 230000036737 immune function Effects 0.000 description 14
- 238000000034 method Methods 0.000 description 11
- 210000004988 splenocyte Anatomy 0.000 description 11
- 241000287828 Gallus gallus Species 0.000 description 10
- 239000003814 drug Substances 0.000 description 10
- 210000002966 serum Anatomy 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 241001494479 Pecora Species 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 8
- 210000004369 blood Anatomy 0.000 description 7
- 239000008280 blood Substances 0.000 description 7
- 230000037396 body weight Effects 0.000 description 7
- 210000004698 lymphocyte Anatomy 0.000 description 7
- 206010020751 Hypersensitivity Diseases 0.000 description 6
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 6
- 208000026935 allergic disease Diseases 0.000 description 6
- 230000007815 allergy Effects 0.000 description 6
- 230000003111 delayed effect Effects 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 210000002540 macrophage Anatomy 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 108010006464 Hemolysin Proteins Proteins 0.000 description 5
- 230000001413 cellular effect Effects 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 238000010790 dilution Methods 0.000 description 5
- 239000012895 dilution Substances 0.000 description 5
- 238000003304 gavage Methods 0.000 description 5
- 239000003228 hemolysin Substances 0.000 description 5
- 210000003024 peritoneal macrophage Anatomy 0.000 description 5
- 239000006285 cell suspension Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 230000002708 enhancing effect Effects 0.000 description 4
- 150000004676 glycans Chemical class 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 210000002784 stomach Anatomy 0.000 description 4
- 230000007306 turnover Effects 0.000 description 4
- 206010011224 Cough Diseases 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 3
- 241000037831 Polygonatum sibiricum Species 0.000 description 3
- 239000012980 RPMI-1640 medium Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000002354 daily effect Effects 0.000 description 3
- 230000034994 death Effects 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 3
- 210000001508 eye Anatomy 0.000 description 3
- 239000000796 flavoring agent Substances 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 210000000822 natural killer cell Anatomy 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 206010018910 Haemolysis Diseases 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 206010033557 Palpitations Diseases 0.000 description 2
- 210000000683 abdominal cavity Anatomy 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000035622 drinking Effects 0.000 description 2
- 239000012636 effector Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 210000003128 head Anatomy 0.000 description 2
- 238000005534 hematocrit Methods 0.000 description 2
- 235000008216 herbs Nutrition 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- 238000002649 immunization Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 239000006210 lotion Substances 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000002107 myocardial effect Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 230000005180 public health Effects 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 230000002992 thymic effect Effects 0.000 description 2
- 210000001541 thymus gland Anatomy 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- UPJVQRZPXLZUET-UHFFFAOYSA-N (10R)-3c,5t,8t-Trihydroxy-10r,13c-dimethyl-17c-((1R:4R)-1,4,5-trimethyl-hexen-(2t)-yl)-(9tH,14tH)-Delta6-tetradecahydro-1H-cyclopenta[a]phenanthren Natural products OC12C=CC3(O)CC(O)CCC3(C)C2CCC2(C)C1CCC2C(C)C=CC(C)C(C)C UPJVQRZPXLZUET-UHFFFAOYSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 206010003694 Atrophy Diseases 0.000 description 1
- 102000040350 B family Human genes 0.000 description 1
- 108091072128 B family Proteins 0.000 description 1
- 235000016068 Berberis vulgaris Nutrition 0.000 description 1
- 241000335053 Beta vulgaris Species 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102100034279 Calcium-binding mitochondrial carrier protein Aralar2 Human genes 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 206010008111 Cerebral haemorrhage Diseases 0.000 description 1
- 206010008132 Cerebral thrombosis Diseases 0.000 description 1
- 206010008909 Chronic Hepatitis Diseases 0.000 description 1
- 241001480006 Clavicipitaceae Species 0.000 description 1
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 description 1
- 108010069514 Cyclic Peptides Proteins 0.000 description 1
- 102000001189 Cyclic Peptides Human genes 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 206010012335 Dependence Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 229920000926 Galactomannan Polymers 0.000 description 1
- 206010019617 Henoch-Schonlein purpura Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 208000031814 IgA Vasculitis Diseases 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 201000001429 Intracranial Thrombosis Diseases 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 244000241838 Lycium barbarum Species 0.000 description 1
- 235000015459 Lycium barbarum Nutrition 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 1
- 208000007443 Neurasthenia Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 235000008737 Polygonatum biflorum Nutrition 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 1
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 1
- 241000208292 Solanaceae Species 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 241001314279 Zoopagales Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 229960003237 betaine Drugs 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 230000002612 cardiopulmonary effect Effects 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 208000013116 chronic cough Diseases 0.000 description 1
- 230000007882 cirrhosis Effects 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 108010084210 citrin Proteins 0.000 description 1
- 210000000078 claw Anatomy 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 description 1
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- VXOZCESVZIRHCJ-KGHQQZOUSA-N ergosterol peroxide Chemical compound O1O[C@@]2(C=C3)[C@@H]4CC[C@H]([C@H](C)/C=C/[C@H](C)C(C)C)[C@@]4(C)CC[C@@H]2[C@]2(C)[C@@]13C[C@@H](O)CC2 VXOZCESVZIRHCJ-KGHQQZOUSA-N 0.000 description 1
- VXOZCESVZIRHCJ-KYQKSOQPSA-N ergosterol peroxide Natural products CC(C)[C@@H](C)C=C[C@H](C)[C@H]1CC[C@@H]2[C@]1(C)CC[C@@H]3[C@@]4(C)CC[C@H](O)C[C@@]45OO[C@@]23C=C5 VXOZCESVZIRHCJ-KYQKSOQPSA-N 0.000 description 1
- LESGHGUKCRHTCP-UHFFFAOYSA-N ergosteryl peroxide Natural products C1C(OO)CCC2(C)C(CCC3(C(C(C)C=CC(C)C(C)C)CCC33)C)C3=CC=C21 LESGHGUKCRHTCP-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000004345 fruit ripening Effects 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 208000026278 immune system disease Diseases 0.000 description 1
- 208000015446 immunoglobulin a vasculitis Diseases 0.000 description 1
- 239000002955 immunomodulating agent Substances 0.000 description 1
- 230000002584 immunomodulator Effects 0.000 description 1
- 229940121354 immunomodulator Drugs 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 206010022437 insomnia Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000007917 intracranial administration Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000000629 knee joint Anatomy 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000003387 muscular Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000035900 sweating Effects 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- DLFVBJFMPXGRIB-UHFFFAOYSA-N thioacetamide Natural products CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a Chinese caterpillar fungus beverage. The Chinese caterpillar fungus beverage is prepared from 3-9% by weight of a sweetener, 0.01-0.04% by weight of cordyceps militaris, 0.01-0.03% by weight of longan aril, 0.01-0.03% by weight of Chinese date, 0.01-0.02% by weight of fruit of Chinese wolfberry, 0.01-0.02% by weight of polygonatum rhizome, 0.005-0.015% by weight of licorice root and the balance water. The Chinese caterpillar fungus beverage has effects of improving immunity.
Description
Technical field
The present invention relates to a kind of preparation method of beverage, especially relate to a kind of cordyceps drink with health role.
Background technology
Cordyceps militaris is Ascomycotina, ergot Zoopagales, the type sepecies of Clavicipitaceae, Cordyceps.Formal name used at school is Cordycepsmilitaris, has another name called northern Chinese caterpillar Fungus, Cordceps militaris etc., has some areas to be also called the Chinese caterpillar fungus that leaps up, and worldwide distribution natural resources quantity is little.Cordyceps militaris and Cordyceps sinensis belong to and belong to together not of the same race, and the research as its effective component of help class Chinese medicine and BA has the history of decades, and its application is more and more extensive.It is reported, its health-care efficacy is similar to Cordyceps sinensis, and 2009 Nian Yuan Ministry of Public Health approval Cordyceps militaris are new resource food, become favorable substitutes and the new resource food of Cordyceps sinensis.Cordyceps militaris is that its chemical analysis is N6-[β-(acetamide formyl) oxygen ethyl] adenosine, Chinese caterpillar fungus cyclic peptide A by stroma (i.e. careless part) and sclerotium (i.e. the corpse part of worm) complex dimerous; Nucleosides, ergosterol peroxide, polysaccharide, several amino acids, electrolytes and minerals.The traditional Chinese medical science think it can tonifying lung cloudy, again can kidney-replenishing, to regulate, Yin Yang balancing simultaneously.Cure mainly and suffer from a deficiency of the kidney, impotence and seminal emission, soreness of waist and knee joint, weak after being ill, chronic cough is weak, and phthisical cough phlegm blood, spontaneous sweating etc. are unique a kind of Chinese medicine that can balance simultaneously, regulate negative and positive.Pharmacological action modern science is proved Cordyceps militaris and is not only had special nutritive value, and has obvious medical value.Wherein especially with cordycepic acid, cordycepin, Cordyceps sinensis polysaccharide.The medical value of the various bioactivators such as Cordyceps sinensis polysaccharide and SOD is the most remarkable.Cordycepic acid (sweet mellow wine) can fall low intracranial pressure significantly, promotes body metabolism, thus makes cerebral hemorrhage and cerebral thrombosis disease be eased.Cordyceps sinensis polysaccharide is a kind of galactomannans of height branch, and it can promote lymphocyte transformation, improves the antibody content of serum IgG and the immunologic function of body, strengthens the anticancer ability pressing down cancer of body self.
Arillus longan is the aril of sapindaceous plant longan, has another name called intelligence development, sweet spleen, longan.Main product in Guangdong, Fujian, Taiwan, Guangxi, Yunnan, Guizhou, the ground such as Sichuan.Pluck when July to October, fruit was ripe, dry or dry, get meat stoning solarization and do not glue to dry and comfortable.Arillus longan is the official drug of the Pharmacopoeia of the People's Republic of China, desirable tonic.The traditional Chinese medical science is thought, longan taste is sweet, warm in nature.Enter the heart, the spleen channel.There is tonifying heart and spleen, nourish blood allay excitement, invigorating the spleen to arrest diarrhea, the effect such as inducing diuresis to reduce edema.Be applicable to the illnesss such as body is after being ill empty, the deficiency of blood is sallow, insufficiency of vital energy and blood, neurasthenia, palpitation and severe palpitation, forgetful insomnia.
Red date is rich in the nutritional labelings such as protein, fat, carbohydrate, carrotene, B family vitamin, vitamin C, citrin and calcium, phosphorus, iron and CAMP.Wherein ascorbic content comes out at the top in fruit, has the laudatory title of vitamin king, has effect of blood-enriching face-nourishing.CAMP contained by red date is the required composition of human body cell energetic supersession, can strengthening muscular strength, dispelling fatigue, hemangiectasis, increase myocardial contractive power, improve myocardial nutrition, having good effect to preventing and treating disease of cardiovascular system; In the traditional Chinese medical science, theory is thought, red date has the effects such as qi-restoratives benefit gas, nourishing blood and tranquilization, strengthening the spleen and stomach, and red date has good therapeutic effect to illnesss such as chronic hepatitis, cirrhosis, anaemia, anaphylactoid purpuras; Red date contains triterpene compound and CAMP, has stronger anticancer, anti-allergic effects.Date can moisten cardiopulmonary, cough-relieving, tonifying five zang organs, it is deficient to control, except stomach addiction gas, support spleen, flat stomach Qi, logical nine orifices, help 12 warps in can also pacifying.
The fruit of Chinese wolfberry, (FRUCTUSLYCII) calls beet red ear pendant ground frame, is the ripening fruits of matrimony vine of solanaceae plant.Pluck when summer, autumn fruit maturation, removing carpopodium, put after shady and cool place dries in the air and corrugate to pericarp, then be exposed to the sun stiff to crust, pulp is soft and get final product.Meet overcast and rainy available low baking temperature to dry.Having various health care functions, is the medicine-food two-purpose food of Ministry of Public Health's approval.LBP-X, betaine is nourishing liver and kidney, benefiting shrewd head.
Sealwort (formal name used at school: Polygonatumsibiricum), has another name called: polygonatum sibiricum Redoute, yellow chicken dish, shaft of a writing brush dish, claw ginseng, tendrilleaf solomonseal rhizome, shank are joined.For HUANGJING ZANYU CAPSULE, rhizome horizontal walk, cylindric, tubercle expands.Impeller is raw, stockless.Medicinal plant, has tonifying spleen, the effect that moistening lung is promoted the production of body fluid.
Along with the quickening of socioeconomic prosperity, rhythm of life, the day by day fierce of competition, modern particularly a middle-aged person will bear the dual heavy burden of family and work, the factor affecting health has a very large change, and the muscle power of long-term excess load, mental labour cause body's immunity decline to such an extent as to cause various diseases.
Modern medicine, cell biology and molecular biological development, people are recognized, and immune disorder not only can produce various diseases, and all have substantial connection with the generation of frequently-occurring disease as tumour, hypertension, diabetes of human senility and the elderly, immunologic function has age_related changes, and namely the age increases, and its immunologic function declines or disorder, result atrophy of thymus gland, T cell loss, thus cause body aging, the lost of life.And Chinese medicine immunomodulator can strengthen the immunologic function of the elderly, closely during the last ten years, chemists obtain multiple compounds from the help class Chinese medicine such as to strengthen the body resistance to consolidate the constitution, and not only can promote the immunologic function of body, and confirm really have very strong immunocompetence.Therefore find and there is good immunoregulation effect, with the medicine of the various diseases such as prevention and therapy tumour, cardiovascular and cerebrovascular.In order to reach daily health-care effect, be badly in need of a kind of instant and the food of safe and reliable integration of drinking and medicinal herbs.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of cordyceps drink, be primary raw material with Cordyceps militaris, be equipped with arillus longan, date, the fruit of Chinese wolfberry, sealwort, the famous and precious genuine integration of drinking and medicinal herbs Chinese medicine of Radix Glycyrrhizae multi-flavor, develop New-generation healthy beverage---Worm grass oral.In side, the fruit of Chinese wolfberry, arillus longan, date, sealwort are all help class Chinese medicine, and to improving human-body sub-health, improving immunity of organisms and having certain effect, Radix Glycyrrhizae works to be in harmonious proportion the property of medicine.
Present invention also offers the preparation method of cordyceps drink, the method is acted on the traditional draft of China and is soaked fresh-keeping extraction process, and in conjunction with the meticulous processing of modern film concentration technique, mouthfeel is excellent, and applicable masses drink.
The present invention is achieved through the following technical solutions:
A kind of cordyceps drink, it is made up of following materials by weight percentage:
Sweetener 3%-9%
Cordyceps militaris 0.01%-0.04%
Arillus longan 0.01%-0.03%
Date 0.01%-0.03%
Fruit of Chinese wolfberry 0.01%-0.02%
Sealwort 0.01%-0.02%
Radix Glycyrrhizae 0.005%-0.015%, surplus is water.
Preferred a kind of cordyceps drink, it is made up of following materials by weight percentage:
Sweetener 4%-8%
Cordyceps militaris 0.02%-0.04%
Arillus longan 0.01%-0.02%
Date 0.01%-0.03%
Fruit of Chinese wolfberry 0.01%-0.02%
Sealwort 0.01%-0.02%
Radix Glycyrrhizae 0.005%-0.015%, surplus is water.
Best a kind of cordyceps drink, it is made up of the raw material of following weight percentage:
Sweetener 7%
Cordyceps militaris 0.035%
Arillus longan 0.02%
Date 0.02%
The fruit of Chinese wolfberry 0.01%
Sealwort 0.01%
Radix Glycyrrhizae 0.010%, surplus is water.
Present invention also offers a kind of preparation method of cordyceps drink, comprise the steps
(1) percentage by weight raw material is got: sweetener 4%-8%, Cordyceps militaris: 0.02%-0.04%, arillus longan 0.01%-0.02%, date 0.01%-0.03%, fruit of Chinese wolfberry 0.01%-0.02%, sealwort 0.01%-0.02%, Radix Glycyrrhizae 0.005%-0.015%, water are for subsequent use;
(2) medicinal material inspissated juice extracts: get Cordyceps militaris, arillus longan, date, the fruit of Chinese wolfberry, sealwort, licorice medicinal materials add water boil, obtain extract I filter after temporary; Add boiling water in the dregs of a decoction after first time extraction, boil, extract II, merge extract I and II, be cooled to below room temperature, cooling fluid is centrifugal, and extracting centrifugal liquid concentrates, and obtains inspissated juice;
(3) product preparing: get inspissated juice 12-20%, heating water fully dissolves; Get sweetener, be dissolved in water into liquid glucose, inspissated juice lysate and liquid glucose are put in blend tank, allocate.
Preferred preparation method, comprises the steps: further
(1) get percentage by weight raw material: sweetener 4%-8%, Cordyceps militaris 0.02%-0.04%, arillus longan 0.01%-0.02%, date 0.01%-0.03%, fruit of Chinese wolfberry 0.01%-0.02%, sealwort 0.01%-0.02%, Radix Glycyrrhizae 0.005%-0.015%, water is for subsequent use;
(2) medicinal material inspissated juice extracts: get Cordyceps militaris, arillus longan, date, the fruit of Chinese wolfberry, sealwort, licorice medicinal materials add 10-15 times of water boil, keep micro-boiling to extract 0.5-3 hour, obtain extract I filter after temporary; 8-12 times of boiling water is added in the dregs of a decoction after first time extraction, boil, keep micro-0.5-3 hour that boils, extract II, merge extract I and II, be cooled to below room temperature, cooling fluid is centrifugal, and extracting centrifugal liquid is crossed film and concentrated, be concentrated into relative density for (more than 15%), obtain inspissated juice, inspissated juice sterilization, filling;
(3) product preparing: get inspissated juice 14-18%, heating water fully dissolves; Get sweetener, be dissolved in water into 30-80% liquid glucose, inspissated juice lysate and liquid glucose are put in blend tank, add water to scale.
Further preferably preparation method, comprises the steps: again
(1) get percentage by weight raw material: sweetener 7%, Cordyceps militaris 0.035%, arillus longan 0.02%, date 0.02%, the fruit of Chinese wolfberry 0.01%, sealwort 0.01%, Radix Glycyrrhizae 0.010%, water is for subsequent use;
(2) get Cordyceps militaris, arillus longan, date, the fruit of Chinese wolfberry, sealwort, licorice medicinal materials add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merge extract I and II, be cooled to less than 20 DEG C, cooling fluid centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, being concentrated into relative density for (more than 15%) namely obtains inspissated juice, inspissated juice at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C;
(3) product preparing: get inspissated juice 16%, heating water fully dissolves; Get sweetener, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to scale.
Product pop can, PET bottle or Tetra Pak that method of the present invention also comprises step (4) deployed are filling, obtain finished product after the assay was approved.
Film described in step of the present invention (2) is reverse osmosis membrane, and membrane aperture requires as only allowing hydrone to pass through.By membrane filtration, retain the fragrance component of plant beverage to greatest extent, be better than the plant beverage produced by traditional handicraft with the plant beverage local flavor of this explained hereafter.
Centrifugal employing disk centrifugal separator described in step of the present invention (2), 4000-6000r/min.
Cordyceps drink of the present invention also can be solid beverage.
Sweetener of the present invention be white granulated sugar, soft white sugar, sucrose, glucose, saccharin sodium, HFCS, honey, sorbierite, sweet mellow wine, honey element, Aspartame, steviol glycoside, alitame, Radix Glycyrrhizae, antierythrite, xylitol, as in sugar alcohol, maltitol, D-mannital one or more.Preferred sweeteners be in white granulated sugar, soft white sugar, sucrose one or more.
Water of the present invention is process water.
Beneficial effect
Beneficial effect of the present invention is set forth by following test example
1 materials and methods
1.1 samples: Worm grass oral (batching of embodiment and preparation), 250ml/ tank, canned, liquid, normal temperature is preserved, and human body recommended daily dosage is 2 tanks/sky, and in the body weight 60kg that is grown up, recommended amounts is 8.33ml/kgBW.The concentrating sample (cycles of concentration is 33.9 times) that this test adopts producer to provide is tested.
1.2 animals used as test and laboratory: the female small white mouse of SPF level Kunming kind, 6 ~ 8 week age (body weight: 18g ~ 22g), thered is provided by Guangdong Medical Lab Animal Center, the quality certification number: SCXK (Guangdong) 2013 ?0002, the certification of fitness is numbered: 44007200021570, and pellet is also provided by Guangdong Medical Lab Animal Center; Animal Lab. is barrier environment, occupancy permit number: SYXK (Guangdong) 2013 ?0011, room temperature 20 ~ 26 DEG C, humidity 40 ~ 70%.
1.3 dosage choice and tested material process and give mode
1.3.1 dosage choice: basic, normal, high three dosage groups are established in this experiment, dosage is 41.7ml/kgBW, 83.3ml/kgBW, 250.0ml/kgBW, conversion is the dosage of concentrating sample is 1.23ml/kgBW, 2.46ml/kgBW, 7.37ml/kgBW, be equivalent to 5,10,30 times of human body recommended amounts, separately establish negative control group.
1.3.2 tested material process and give mode: measure concentrating sample 73.7ml during experiment, be mixed with the sample liquid of 200.0ml with pure water, for high dose group animal gavage; From high dose group sample liquid, get 50.0ml, be diluted to 150.0ml with pure water, in dosage treated animal gavage; Therefrom get 50.0ml in dosage group sample liquid, be diluted to 100.0ml with pure water, for low dose group animal gavage.Every day measures gavage animal by 0.2ml/10gBW and gives tested material.
1.4 key instruments and reagent: CO
2incubator,
dT type cell counter, SOFTmaxPRO ELIASA, superclean bench, spectrophotometer, inverted microscope, microscope, incubator, slide measure, RPMI1640 culture medium, sheep red blood cell (SRBC), MTT and ConA etc.
1.5 test methods: after animal is quarantined three days in laboratory conditions, are divided into I, II, III, IV batch by animal, often criticize 40 animals and be divided into four groups at random, often organize 10, every day, gavage gave tested material, and to sample amount according to body weight increase and decrease adjustment weekly, experimental period is 4 weeks.
1.5.1 mouse delayed allergy test (sufficient sole of the foot thickness increases method) (the Ith batch of animal)
The 4th day immune animal before experiment terminates, by 2% (v/v) sheep red blood cell (SRBC) lumbar injection 0.2ml sensitized animal, left back sufficient sole of the foot portion thickness is measured after 5 days, then in this place's hypodermic injection 20% (v/v) sheep red blood cell (SRBC) (the every mouse of 20 μ l/), inject and measure left back sufficient sole of the foot portion thickness three times in latter 24 hours, obtain mean value.
The mouse spleen lymphocyte conversion test (mtt assay) (the IIth batch of animal) of 1.5.2ConA inducing
Prepared by splenocyte suspension: asepticly get spleen, is placed in the little plate filling appropriate aseptic Hank ' s liquid, is torn up by spleen gently, make individual cells suspension with embedding son.Filter through 200 eye mesh screens, Hank ' s liquid washes 3 times, each centrifugal 10min (1000r/min).Then cell is suspended in the complete culture solution of 2ml, uses
dT cell counter counting splenocyte, adjustment cell concentration is 3 × 10
6individual/ml.
Lymphproliferation response: add in 24 well culture plates by a cell suspension point holes, every hole 1ml, a hole adds 75 μ lConA liquid (being equivalent to 7.5ug/ml), and 5%CO in contrast, is put in another hole
2, cultivate 72h for 37 DEG C.Cultivation terminates first 4 hours, and every hole sucks supernatant 0.7ml, adds 0.7ml serum-free RPMI1640 nutrient solution, adds MTT (5mg/ml) 50 μ l/ hole simultaneously, continues to cultivate 4h.After cultivation terminates, every hole adds 1ml acid isopropyl alcohol, and piping and druming mixing, makes purple crystal dissolve, and divides and is added in 96 well culture plates, makes the Duplicate Samples in 2 holes, measures OD value with 570nm wavelength.
1.5.3 mice serum hemolysin titer determination (HD50 value method) (the Ith batch of animal)
The 4th day immune animal before experiment terminates, the sheep red blood cell (SRBC) 0.2ml of every lumbar injection 2%, within 5 days, extract eyeball blood sampling afterwards, separation of serum is for subsequent use.Hemolytic reaction: with SA buffer solution by serum-dilution 100 times, the serum 1ml after dilution is put in vitro, adds 10%SRBC0.5ml successively, complement 1ml (pressing 1:8 dilution with SA liquid), separately establishes the control tube of not increase serum; Put 30min in 37 DEG C of incubators, ice bath cessation reaction, centrifugal (2000r/min) 10min, gets supernatant 1ml, adds Dou Shi reagent 3ml, gets 10%SRBC0.25ml simultaneously and adds Dou Shi reagent to 4ml, fully mix; After placing 10min, measure each pipe OD value respectively in 540nm place.
1.5.4 mouse boosting cell antibody tormation test (the IIth batch of animal)
Immune animal: the sheep blood getting defiber, with brine 3 times, centrifugal (2000r/min) 10min, hematocrit SRBC physiological saline is made into the cell suspension of 2% (v/v) at every turn, every mouse lumbar injection 0.2ml.
Prepared by splenocyte suspension: put to death by the mouse cervical dislocation of SRBC immunity after 4 days, take out spleen, be placed on fill Hank ' s liquid little plate in, grind spleen gently, make cell suspension, filter through 200 eye mesh screens, centrifugal (1000r/min) 10min, wash 2 times with Hank ' s liquid, finally cell is suspended in 5mlRPMI1640 nutrient solution, use
dT cell counter counting splenocyte, and cell concentration is adjusted to 5 × 10
6individual/ml.
The mensuration of plaque: after the culture medium heating for dissolving of top layer, put 45 ~ 50 DEG C of water bath heat preservations, with equivalent pH7.2 ~ 7.4, Hank ' the s liquid mixing of 2 times of concentration, packing small test tube, often pipe 0.5ml, 50 μ l10%SRBC (v/v are added again in pipe, use SA buffer), 25 μ l splenocyte suspensions, rapid mixing, be poured on the slide of own brush agarose thin layer, do parallel plate, after agar solidification, slide level being buckled is placed on horse, put into CO2gas incubator and hatch 1 ~ 1.5h, then join in slide frame groove with the complement (1:8) of SA buffer solution dilution, after continuing incubation 1 ~ 1.5h, counting hemolysis plaque number.
1.5.5 mouse carbonic clearance test (the IIIth batch of animal)
With the india ink of 1:4 dilution after last administration, by every mouse 10g body weight 0.1ml tail vein injection, timing immediately, blood 20 μ l is got from angular vein clump in 2,10 minutes respectively in interval, adds 2mlNa
2cO
3in solution, survey OD value with spectrophotometer in 600nm wavelength place, use Na
2cO
3solution does blank, puts to death mouse, gets liver, spleen is weighed, and calculates phagocytic index.
1.5.6 Turnover of Mouse Peritoneal Macrophages engulfs chicken red blood cell test (the IVth batch of animal)
The activation of mouse macrophage: test and inject 2% hematocrit sheeps blood erythrocyte 0.2ml to every mouse peritoneal in first 4 days.Put to death mouse with cervical dislocation, lumbar injection only adds Hank ' the s liquid 4ml/ of calf serum, rubs belly gently 20 times, fully to wash out peritoneal macrophage, then stomach wall is cut off an osculum, draws abdominal cavity washing lotion 2ml in vitro with glue head straw.Add with 1ml sample injector absorption abdominal cavity washing lotion 0.5ml and fill 0.5ml1% chicken red blood cells suspension in vitro, mixing.Draw 0.5ml mixed liquor with syringe, add in the agar circle of slide.15 ~ 20 minutes are hatched for 37 DEG C in placement incubator.Hatch rapid physiological saline after terminating to be washed out by non-attached cell, in methanol solution, fix 1 minute, Giemsa liquid dyes 15 minutes.Clean with distilled water flushing, dry, by 40 × microscopic counting phagocytic rate and phagocytic index.Phagocytic rate is in every 100 macrophages, engulfs the percentage shared by macrophage of chicken red blood cell: phagocytic index is the number of average each macrophage phagocytic chicken red blood cell.
1.5.7NK cytoactive detection (LDH method) (the IIth batch of animal)
The preparation (effector cell) of splenocyte suspension: asepticly get spleen, is placed in the little plate filling appropriate aseptic Hank ' s liquid, is torn up by spleen gently, make single cell suspension with tweezers.Filter through 200 eye mesh screens, Hank ' s liquid washes 3 times, each centrifugal 10min (1000r/min).Then cell is suspended in the complete culture solution of 2ml, uses
dT cell counter counting splenocyte, finally adjusting cell concentration with RPMI1640 complete culture solution is 2 × 10
7individual/ml.
NK cytoactive detects: getting concentration is 4 × 10
5the YAC of individual/ml ?1 target cell and each 100 μ l of effector cell (effect target is than 50:1), add U-shaped 96 well culture plates; Target cell Spontaneous release hole adds target cell and each 100 μ l of nutrient solution, and the maximum release aperture of target cell adds target cell and each 100 μ l of 1%NP40; Above-mentionedly everyly all establish three multiple holes, in 37 DEG C, 5%CO
2cultivate 4h in incubator, then by 96 well culture plates with the centrifugal 5min of 1500r/min, every hole is drawn in 96 well culture plates at the bottom of supernatant 100 μ l horizontalization, add LDH matrix liquid 100 μ l simultaneously, reaction 3min, every hole adds 1mol/LHCl30 μ l, and at enzyme connection, instrument 492nm place measures OD value.
1.6 test data statistics: adopt variance analysis, first carry out homogeneity test of variance by the program of variance analysis, variance is neat, calculates F value, F value <F
0.05, conclusion: no significant difference between each group mean; F value>=F
0.05, P≤0.05, adds up with the comparative approach between two of mean between multiple experimental group and a control group; Suitable variable transitions is carried out to the data of abnormal or heterogeneity of variance, after meeting normal state or variance and requiring together, adds up by the data after conversion; If do not reach normal state or the neat object of variance after variable transitions yet, use rank test instead and add up.
1.7 results judge
Develop immunitypty function judges: in cellular immune function, humoral immune function, monokaryon-macrophage function, NK cytoactive four, result is positive in any two, can judge this given the test agent develop immunitypty function.
1.7.1 cellular immune function result judges: two experimental results in cellular immune function assay project are the positive, or two dosage group results of any one experiment are positive, can judge that cellular immune function assay result is positive.
1.7.2 humoral immune function result judges: two experimental results in humoral immune function mensuration project are the positive, or two dosage group results of any one experiment are positive, can judge that humoral immune function measurement result is positive.
1.7.3 monokaryon-macrophage function result judges: two experimental results in monokaryon-macrophage function mensuration project are the positive, or two dosage group results of any one experiment are positive, can judge that monokaryon-macrophage function result is positive.
1.7.4NK cytoactive result judges: the above result of a dosage group of NK cytoactive detection experiment is positive, can judge that NK cytoactive result is positive.
2 results
2.1 Worm grass orals are on the impact of Mouse Weight: serum hemolysin and delayed allergy group are in table 1, and lymphocyte transformation, NK determination of activity, antibody tormation group are in table 2, and carbonic clearance experimental group, in table 3, engulfs chicken red blood cell group in table 4.Comparatively, difference there are no significant meaning, shows that the body weight growth of Worm grass oral to mouse has no significant effect to each dosage group each phase body weight weight ratio in period corresponding to control group.
Table 1. Worm grass oral on the impact of Mouse Weight (the Ith batch of animal, n=10,
)
Table 2. Worm grass oral on the impact of Mouse Weight (the IIth batch of animal, n=10,
)
Table 3. Worm grass oral on the impact of Mouse Weight (the IIIth batch of animal, n=10,
)
Table 4. Worm grass oral on the impact of Mouse Weight (the IVth batch of animal, n=10,
)
2.2 Worm grass orals are on the impact of mouse spleen weight and thymic weight: each dosage group internal organs/body weight ratio compares with control group, difference there are no significant meaning (see table 5).Show that Worm grass oral has no significant effect the spleen weight of mouse and thymic weight.
Table 5. Worm grass oral on the impact of mouse thymus, spleen weight (n=10,
)
2.3 Worm grass orals are on the impact of the delayed allergy of mouse: the middle and high dosage group foot sole of the foot thickens value higher than control group, and difference has significant (see table 6).Show Worm grass oral to the delayed allergy result of the test of mouse for positive.
Table 6. Worm grass oral is on the impact of mouse delayed allergy
2.4 Worm grass orals are on the impact of the mouse spleen lymphocyte conversion reaction that ConA induces: each dosage group optical density difference compares with control group, difference there are no significant meaning (see table 7).Show Worm grass oral to mouse spleen lymphocyte conversion test result for negative.
Table 7. Worm grass oral is on the impact of mouse spleen lymphocyte conversion reaction
2.5 Worm grass orals are on the impact of animal subject serum hemolysin HD50 value: the serum hemolysin HD50 value of basic, normal, high dosage group is all higher than control group, and difference has significant (see table 8).Show Worm grass oral to animal subject serolysin test result for positive.
Table 8. Worm grass oral is on the impact of mice serum hemolysin HD50 value
2.6 Worm grass orals are on the impact of animal subject splenocyte antibody tormation level: the splenocyte antibody tormation level of each dosage group compares with control group, difference there are no significant meaning (see table 9).Show Worm grass oral to animal subject splenocyte antibody tormation level determination result for negative.
Table 9. Worm grass oral is on the impact of mouse boosting cell antibody tormation level
2.7 Worm grass orals are on the impact of mouse carbonic clearance phagocytic function: each dosage group carbonic clearance phagocytic index compares with control group, difference there are no significant meaning (see table 10).Show that the carbonic clearance phagocytosis result of the test of Worm grass oral is for negative.
Table 10. Worm grass oral is on the impact of mouse carbonic clearance phagocytic function
2.8 Worm grass orals engulf the impact of chicken red blood cell phagocytic function to Turnover of Mouse Peritoneal Macrophages: phagocytic percentage conversion value and the phagocytic index of each dosage group compare with control group, difference there are no significant meaning (see table 11).Show that the Turnover of Mouse Peritoneal Macrophages of Worm grass oral engulfs chicken red blood cell result of the test for negative.
Table 11. Worm grass oral on Turnover of Mouse Peritoneal Macrophages engulf chicken red blood cell phagocytic function impact (n=10,
)
Phagocytic percentage conversion value: X=Sin
?1(p)
1/2
2.9 Worm grass orals are on the impact of animal subject NK cytoactive: the NK cytoactive of each dosage group compares with control group, difference there are no significant meaning (see table 12).Show Worm grass oral to animal subject NK cell activity assays result for negative.
Table 12. Worm grass oral on the impact of NK cells in mice activity (n=10,
)
NK cytoactive conversion value: X=Sin
?1(p)
1/2
3 brief summaries
To SPF level Kunming mouse per os to give respectively every day Worm grass oral concentrating sample (cycles of concentration is 33.9 times) 1.23,2.46,7.37ml/kgBW, be equivalent to Worm grass oral 41.7ml/kgBW, 83.3ml/kgBW, 250.0ml/kgBW, for 5,10,30 times of Worm grass oral recommended daily dosage, totally 4 weeks, result shows: the mouse delayed allergy result of the test of sheep red blood cell (SRBC) induction is positive, and given the test agent has the effect of enhancing cellular immune function; After sheep red blood cell (SRBC) immunization of animal subjects, serolysin test result is positive, and given the test agent has enhancing body fluid immunity function; Carbonic clearance test and macrophage phagocytic chicken red blood cell result of the test negative, given the test agent do not have enhancing Dan He ?macrophage function effect; NK cell activity assays is negative, and given the test agent does not have the effect of enhancing NK cytoactive; The mouse spleen lymphocyte proliferation result of the test of ConA induction is negative; After sheep red blood cell (SRBC) immunization of animal subjects, splenocyte antibody tormation result of the test is negative.
Judge according to " health food inspection and assessment technical specification " develop immunitypty functional evaluation standard, Worm grass oral has develop immunitypty function
The cordyceps drink prepared by formula of the present invention and technique keeps lower apparent viscosity, thus make delicate mouthfeel, mellow, tasty and refreshing plant beverage, has brand-new peculiar flavour, good mouthfeel, not only meet the standard of national tea beverage, also there is certain health-care effect, to improving human-body sub-health, improving immunity of organisms and there is certain effect.
Composition in right of the present invention and the composition of embodiment according to actual production, can zoom in or out ratio simultaneously.
Detailed description of the invention
Detailed description of the invention is only used to content of the present invention is described, is not the further restriction to claims of the present invention.
Embodiment 1
Get Cordyceps militaris 0.35kg, arillus longan 0.2kg, date 0.2kg, fruit of Chinese wolfberry 0.1kg, sealwort 0.1kg, Radix Glycyrrhizae 0.1kg medicinal material add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 16kg, heating water fully dissolves; Get white granulated sugar 70kg, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 2
Get Cordyceps militaris 0.35kg, arillus longan 0.2kg, date 0.2kg, fruit of Chinese wolfberry 0.1kg, sealwort 0.1kg, Radix Glycyrrhizae 0.1kg medicinal material add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 14kg, heating water fully dissolves; Get white granulated sugar 90kg, be dissolved in water into 80% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 3
Get Cordyceps militaris 0.4kg, arillus longan 0.3kg, date 0.3kg, fruit of Chinese wolfberry 0.2kg, sealwort 0.2kg, Radix Glycyrrhizae 0.15kg medicinal material add 15 times of water boils, keep micro-extraction 0.5 hour of boiling, obtain extract I filter after temporary; Add 12 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 18kg, heating water fully dissolves; Get white granulated sugar 80kg, be dissolved in water into 70% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 4
Get Cordyceps militaris 0.2kg, arillus longan 0.1kg, date 0.1kg, fruit of Chinese wolfberry 0.1kg, sealwort 0.1kg, Radix Glycyrrhizae 0.05kg medicinal material add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 16kg, heating water fully dissolves; Get white granulated sugar 40kg, be dissolved in water into 30% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 5
Get Cordyceps militaris 0.4kg, arillus longan 0.2kg, date 0.3kg, fruit of Chinese wolfberry 0.2kg, sealwort 0.2kg, Radix Glycyrrhizae 0.15kg medicinal material add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 16kg, heating water fully dissolves; Get white granulated sugar 70kg, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 6
Get Cordyceps militaris 0.35kg, arillus longan 0.2kg, date 0.2kg, fruit of Chinese wolfberry 0.1kg, sealwort 0.1kg, Radix Glycyrrhizae 0.1kg medicinal material add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 16kg, heating water fully dissolves; Get maltose 70kg, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 7
Get Cordyceps militaris 0.35kg, arillus longan 0.2kg, date 0.2kg, fruit of Chinese wolfberry 0.1kg, sealwort 0.1kg, Radix Glycyrrhizae 0.1kg medicinal material add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 16kg, heating water fully dissolves; Get glucose 70kg, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 8
Get Cordyceps militaris 0.1kg, arillus longan 0.1kg, date 0.1kg, fruit of Chinese wolfberry 0.1kg, sealwort 0.1kg, Radix Glycyrrhizae 0.05kg medicinal material add 8 times of water boils, keep micro-extraction 3 hours of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 16kg, heating water fully dissolves; Get white granulated sugar 70kg, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 9
Get Cordyceps militaris 0.35kg, arillus longan 0.2kg, date 0.2kg, fruit of Chinese wolfberry 0.1kg, sealwort 0.1kg, Radix Glycyrrhizae 0.1kg medicinal material add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 12kg, heating water fully dissolves; Get soft white sugar 70kg, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 10
Get Cordyceps militaris 0.35kg, arillus longan 0.2kg, date 0.2kg, fruit of Chinese wolfberry 0.1kg, sealwort 0.1kg, Radix Glycyrrhizae 0.1kg medicinal material add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 20kg, heating water fully dissolves; Get sucrose 70kg, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Claims (10)
1. a cordyceps drink, it is made up of following materials by weight percentage:
Sweetener 3%-9%
Cordyceps militaris 0.01%-0.04%
Arillus longan 0.01%-0.03%
Date 0.01%-0.03%
Fruit of Chinese wolfberry 0.01%-0.02%
Sealwort 0.01%-0.02%
Radix Glycyrrhizae 0.005%-0.015%, surplus is water.
2. a kind of cordyceps drink as claimed in claim 1, it is made up of following materials by weight percentage:
Sweetener 4%-8%
Cordyceps militaris 0.02%-0.04%
Arillus longan 0.01%-0.02%
Date 0.01%-0.03%
Fruit of Chinese wolfberry 0.01%-0.02%
Sealwort 0.01%-0.02%
Radix Glycyrrhizae 0.005%-0.015%, surplus is water.
3. cordyceps drink as claimed in claim 2, it is made up of the raw material of following weight percentage:
Sweetener 7%
Cordyceps militaris 0.035%
Arillus longan 0.02%
Date 0.02%
The fruit of Chinese wolfberry 0.01%
Sealwort 0.01%
Radix Glycyrrhizae 0.010%, surplus is water.
4. the cordyceps drink as described in claim 1-3 any one, is characterized in that: described sweetener be white granulated sugar, soft white sugar, sucrose, glucose, saccharin sodium, HFCS, honey, sorbierite, sweet mellow wine, honey element, Aspartame, steviol glycoside, alitame, Radix Glycyrrhizae, antierythrite, xylitol, as sugar alcohol, maltitol, D ?in mannitol one or more; Described sweetener be in white granulated sugar, soft white sugar, sucrose one or more.
5. a preparation method for cordyceps drink, is characterized in that, comprises the steps:
(1) percentage by weight raw material is got: sweetener 4%-8%, Cordyceps militaris: 0.02%-0.04%, arillus longan 0.01%-0.02%,
Date 0.01%-0.03%, fruit of Chinese wolfberry 0.01%-0.02%, sealwort 0.01%-0.02%, Radix Glycyrrhizae 0.005%-0.015%, water are for subsequent use;
(2) medicinal material inspissated juice extracts: get Cordyceps militaris, arillus longan, date, the fruit of Chinese wolfberry, sealwort, licorice medicinal materials add water boil, obtain extract I filter after temporary; Add boiling water in the dregs of a decoction after first time extraction, boil, extract II, merge extract I and II, be cooled to below room temperature, cooling fluid is centrifugal, and extracting centrifugal liquid concentrates, and obtains inspissated juice;
(3) product preparing: get inspissated juice 12-20%, heating water fully dissolves; Get sweetener, be dissolved in water into liquid glucose, inspissated juice lysate and liquid glucose are put in blend tank, allocate.
6. preparation method as claimed in claim 5, is characterized in that, comprise the steps:
(1) percentage by weight raw material is got: sweetener 4%-8%, Cordyceps militaris 0.02%-0.04%, arillus longan 0.01%-0.02%, date 0.01%-0.03%, fruit of Chinese wolfberry 0.01%-0.02%, sealwort 0.01%-0.02%, Radix Glycyrrhizae 0.005%-0.015%, water are for subsequent use;
(2) medicinal material inspissated juice extracts: get Cordyceps militaris, arillus longan, date, the fruit of Chinese wolfberry, sealwort, licorice medicinal materials add 10-15 times of water boil, keep micro-boiling to extract 0.5-3 hour, obtain extract I filter after temporary; Add 8-12 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-0.5-3 hour that boils, extract II, merge extract I and II, be cooled to below room temperature, cooling fluid is centrifugal, and extracting centrifugal liquid is crossed film and concentrated, being concentrated into relative density is more than 15%, obtains inspissated juice, and inspissated juice sterilization is filling;
(3) product preparing: get inspissated juice 14-18%, heating water fully dissolves; Get sweetener, be dissolved in water into 30-80% liquid glucose, inspissated juice lysate and liquid glucose are put in blend tank, add water to scale.
7. preparation method as claimed in claim 6, is characterized in that, comprise the steps:
(1) get percentage by weight raw material: sweetener 7%, Cordyceps militaris 0.035%, arillus longan 0.02%, date 0.02%, the fruit of Chinese wolfberry 0.01%, sealwort 0.01%, Radix Glycyrrhizae 0.010%, water is for subsequent use;
(2) get Cordyceps militaris, arillus longan, date, the fruit of Chinese wolfberry, sealwort, licorice medicinal materials add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merge extract I and II, be cooled to less than 20 DEG C, cooling fluid is centrifugal, extracting centrifugal liquid is crossed film and is concentrated, being concentrated into relative density is more than 15% namely obtain inspissated juice, inspissated juice at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C;
(3) product preparing: get inspissated juice 16%, heating water fully dissolves; Get sweetener, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to scale.
8. the preparation method as described in claim 5-7 any one, is characterized in that: the product pop can, PET bottle or the Tetra Pak that comprise step (4) deployed are filling, obtains finished product after the assay was approved.
9. preparation method as claimed in claim 6, is characterized in that: the film described in step (2) is reverse osmosis membrane; Described centrifugal employing disk centrifugal.
10. cordyceps drink according to claim 1 strengthens the application on body immunity health products or food in preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510776271.0A CN105360824B (en) | 2014-11-14 | 2015-11-12 | A kind of cordyceps drink and preparation method thereof |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410650345 | 2014-11-14 | ||
| CN2014106503451 | 2014-11-14 | ||
| CN201510776271.0A CN105360824B (en) | 2014-11-14 | 2015-11-12 | A kind of cordyceps drink and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105360824A true CN105360824A (en) | 2016-03-02 |
| CN105360824B CN105360824B (en) | 2018-11-23 |
Family
ID=55363912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510776271.0A Active CN105360824B (en) | 2014-11-14 | 2015-11-12 | A kind of cordyceps drink and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105360824B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105747046A (en) * | 2016-03-11 | 2016-07-13 | 辽宁隆维生物科技有限公司 | Cordyceps militaris liquid beverage and preparation method thereof |
| CN107304398A (en) * | 2017-05-09 | 2017-10-31 | 张帆 | A kind of preparation technology for the Cordyceps militaris magma product for improving immunity |
| CN108013269A (en) * | 2016-11-02 | 2018-05-11 | 浙江泛亚生物医药股份有限公司 | A kind of cicada fungus siberian solomonseal beverage and preparation method thereof |
| CN109222082A (en) * | 2018-10-20 | 2019-01-18 | 广州王老吉药业股份有限公司 | A kind of Chinese medicine composition is preparing the purposes in antifatigue food or health care product |
| CN109463754A (en) * | 2018-10-20 | 2019-03-15 | 广州王老吉药业股份有限公司 | A kind of Chinese medicine composition is preparing the purposes in antioxidant food or health care product |
| CN109463504A (en) * | 2018-12-27 | 2019-03-15 | 山西省农业科学院食用菌研究所 | A kind of cordyceps flower function tea beverage and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101991154A (en) * | 2009-08-21 | 2011-03-30 | 康美药业股份有限公司 | Vegetable beverage formulation and manufacturing method thereof |
| TW201132290A (en) * | 2010-03-24 | 2011-10-01 | Abundant Seedling Biotech Corp | Method of manufacturing extraction fluid of Agaricus blazei Murill |
| CN102524888A (en) * | 2012-01-04 | 2012-07-04 | 任文武 | Method for preparing Cordyceps militaris sugar-free beverage by microwave extraction |
| CN103462022A (en) * | 2013-09-24 | 2013-12-25 | 郑剑峰 | Cordyceps militaris nourishing paste and preparation method thereof |
| CN104068443A (en) * | 2014-06-10 | 2014-10-01 | 南京泽朗农业发展有限公司 | Solid beverage and preparation method thereof |
| CN104397291A (en) * | 2014-12-04 | 2015-03-11 | 广州王老吉大健康产业有限公司 | Preparation method of concentrated juice of herbal tea |
-
2015
- 2015-11-12 CN CN201510776271.0A patent/CN105360824B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101991154A (en) * | 2009-08-21 | 2011-03-30 | 康美药业股份有限公司 | Vegetable beverage formulation and manufacturing method thereof |
| TW201132290A (en) * | 2010-03-24 | 2011-10-01 | Abundant Seedling Biotech Corp | Method of manufacturing extraction fluid of Agaricus blazei Murill |
| CN102524888A (en) * | 2012-01-04 | 2012-07-04 | 任文武 | Method for preparing Cordyceps militaris sugar-free beverage by microwave extraction |
| CN103462022A (en) * | 2013-09-24 | 2013-12-25 | 郑剑峰 | Cordyceps militaris nourishing paste and preparation method thereof |
| CN104068443A (en) * | 2014-06-10 | 2014-10-01 | 南京泽朗农业发展有限公司 | Solid beverage and preparation method thereof |
| CN104397291A (en) * | 2014-12-04 | 2015-03-11 | 广州王老吉大健康产业有限公司 | Preparation method of concentrated juice of herbal tea |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105747046A (en) * | 2016-03-11 | 2016-07-13 | 辽宁隆维生物科技有限公司 | Cordyceps militaris liquid beverage and preparation method thereof |
| CN105747046B (en) * | 2016-03-11 | 2019-05-07 | 辽宁隆维生物科技有限公司 | A kind of Cordyceps militaris liquid drink and preparation method thereof |
| CN108013269A (en) * | 2016-11-02 | 2018-05-11 | 浙江泛亚生物医药股份有限公司 | A kind of cicada fungus siberian solomonseal beverage and preparation method thereof |
| CN107304398A (en) * | 2017-05-09 | 2017-10-31 | 张帆 | A kind of preparation technology for the Cordyceps militaris magma product for improving immunity |
| CN109222082A (en) * | 2018-10-20 | 2019-01-18 | 广州王老吉药业股份有限公司 | A kind of Chinese medicine composition is preparing the purposes in antifatigue food or health care product |
| CN109463754A (en) * | 2018-10-20 | 2019-03-15 | 广州王老吉药业股份有限公司 | A kind of Chinese medicine composition is preparing the purposes in antioxidant food or health care product |
| CN109463504A (en) * | 2018-12-27 | 2019-03-15 | 山西省农业科学院食用菌研究所 | A kind of cordyceps flower function tea beverage and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105360824B (en) | 2018-11-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103039671B (en) | Chinese caterpillar fungus instant tea | |
| CN105360824B (en) | A kind of cordyceps drink and preparation method thereof | |
| CN102827750A (en) | Maca health care liquor for improving organism immunity and sexuality | |
| CN107581619A (en) | A kind of antifatigue anti senility sea cucumber oyster peptide complex capsule and preparation method thereof | |
| CN106244371B (en) | A kind of hypoglycemic rhizoma polygonati grape health care wine and its production technology | |
| CN104041903A (en) | Selenium-rich cyclocarya paliurus beverage and preparation method thereof | |
| CN107348090B (en) | Ginseng candy capable of preserving health and tonifying kidney and preparation method thereof | |
| CN101856120A (en) | Health food for improving immunity of human body and preparation method thereof | |
| CN102424625A (en) | Edible and medical fungi blackening agent, its preparation method and application | |
| CN102697047A (en) | Method for preparing oral liquid of Chinese wolfberry | |
| CN104336174A (en) | Rhodiola rosea and milk vinegar contained green tea health beverage | |
| CN104388236B (en) | A kind of grape wine and preparation method and application | |
| CN109528814A (en) | A kind of probiotics and its preparation method and application of lactobacillus-fermented Radix Astragali | |
| CN108041391A (en) | A kind of Black people join theanine drink and preparation method thereof | |
| CN107647395A (en) | It is a kind of that there is health food for improving immunity function and preparation method thereof | |
| CN101574151A (en) | Nutritional food with function of strengthening immunity and preparation method thereof | |
| CN104928140A (en) | Compound fermentation type winter-jujube health care wine and production technology thereof | |
| CN107432911B (en) | Ginseng buccal tablet for preserving health and tonifying kidney and preparation method thereof | |
| CN103082240B (en) | Carrot-ginseng processing method | |
| CN102229877B (en) | Brewing method for pine pollen wine | |
| CN104312875A (en) | Preparation method of millet tonic wine | |
| CN107223966B (en) | Roxburgh rose and licorice oral liquid for improving immunity and preparation method thereof | |
| CN109486613A (en) | One kind is for health liquor and its beverage and preparation method | |
| CN105145931A (en) | Hypoglycemic tea and making method thereof | |
| CN104222278A (en) | Milk vinegar and green tea health drink with weight-losing function |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |