CN105360824A - Chinese caterpillar fungus beverage and preparation method thereof - Google Patents

Chinese caterpillar fungus beverage and preparation method thereof Download PDF

Info

Publication number
CN105360824A
CN105360824A CN201510776271.0A CN201510776271A CN105360824A CN 105360824 A CN105360824 A CN 105360824A CN 201510776271 A CN201510776271 A CN 201510776271A CN 105360824 A CN105360824 A CN 105360824A
Authority
CN
China
Prior art keywords
water
inspissated juice
sweetener
fruit
date
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201510776271.0A
Other languages
Chinese (zh)
Other versions
CN105360824B (en
Inventor
方广宏
郑荣波
黄晓丹
朱焕容
和海龙
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GUANGZHOU WANGLAOJI FOOD Co Ltd
WANGLAOJI PHARMACEUTICAL CO Ltd GUANGZHOU CITY
Original Assignee
GUANGZHOU WANGLAOJI FOOD Co Ltd
WANGLAOJI PHARMACEUTICAL CO Ltd GUANGZHOU CITY
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GUANGZHOU WANGLAOJI FOOD Co Ltd, WANGLAOJI PHARMACEUTICAL CO Ltd GUANGZHOU CITY filed Critical GUANGZHOU WANGLAOJI FOOD Co Ltd
Priority to CN201510776271.0A priority Critical patent/CN105360824B/en
Publication of CN105360824A publication Critical patent/CN105360824A/en
Application granted granted Critical
Publication of CN105360824B publication Critical patent/CN105360824B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention discloses a Chinese caterpillar fungus beverage. The Chinese caterpillar fungus beverage is prepared from 3-9% by weight of a sweetener, 0.01-0.04% by weight of cordyceps militaris, 0.01-0.03% by weight of longan aril, 0.01-0.03% by weight of Chinese date, 0.01-0.02% by weight of fruit of Chinese wolfberry, 0.01-0.02% by weight of polygonatum rhizome, 0.005-0.015% by weight of licorice root and the balance water. The Chinese caterpillar fungus beverage has effects of improving immunity.

Description

A kind of cordyceps drink and preparation method thereof
Technical field
The present invention relates to a kind of preparation method of beverage, especially relate to a kind of cordyceps drink with health role.
Background technology
Cordyceps militaris is Ascomycotina, ergot Zoopagales, the type sepecies of Clavicipitaceae, Cordyceps.Formal name used at school is Cordycepsmilitaris, has another name called northern Chinese caterpillar Fungus, Cordceps militaris etc., has some areas to be also called the Chinese caterpillar fungus that leaps up, and worldwide distribution natural resources quantity is little.Cordyceps militaris and Cordyceps sinensis belong to and belong to together not of the same race, and the research as its effective component of help class Chinese medicine and BA has the history of decades, and its application is more and more extensive.It is reported, its health-care efficacy is similar to Cordyceps sinensis, and 2009 Nian Yuan Ministry of Public Health approval Cordyceps militaris are new resource food, become favorable substitutes and the new resource food of Cordyceps sinensis.Cordyceps militaris is that its chemical analysis is N6-[β-(acetamide formyl) oxygen ethyl] adenosine, Chinese caterpillar fungus cyclic peptide A by stroma (i.e. careless part) and sclerotium (i.e. the corpse part of worm) complex dimerous; Nucleosides, ergosterol peroxide, polysaccharide, several amino acids, electrolytes and minerals.The traditional Chinese medical science think it can tonifying lung cloudy, again can kidney-replenishing, to regulate, Yin Yang balancing simultaneously.Cure mainly and suffer from a deficiency of the kidney, impotence and seminal emission, soreness of waist and knee joint, weak after being ill, chronic cough is weak, and phthisical cough phlegm blood, spontaneous sweating etc. are unique a kind of Chinese medicine that can balance simultaneously, regulate negative and positive.Pharmacological action modern science is proved Cordyceps militaris and is not only had special nutritive value, and has obvious medical value.Wherein especially with cordycepic acid, cordycepin, Cordyceps sinensis polysaccharide.The medical value of the various bioactivators such as Cordyceps sinensis polysaccharide and SOD is the most remarkable.Cordycepic acid (sweet mellow wine) can fall low intracranial pressure significantly, promotes body metabolism, thus makes cerebral hemorrhage and cerebral thrombosis disease be eased.Cordyceps sinensis polysaccharide is a kind of galactomannans of height branch, and it can promote lymphocyte transformation, improves the antibody content of serum IgG and the immunologic function of body, strengthens the anticancer ability pressing down cancer of body self.
Arillus longan is the aril of sapindaceous plant longan, has another name called intelligence development, sweet spleen, longan.Main product in Guangdong, Fujian, Taiwan, Guangxi, Yunnan, Guizhou, the ground such as Sichuan.Pluck when July to October, fruit was ripe, dry or dry, get meat stoning solarization and do not glue to dry and comfortable.Arillus longan is the official drug of the Pharmacopoeia of the People's Republic of China, desirable tonic.The traditional Chinese medical science is thought, longan taste is sweet, warm in nature.Enter the heart, the spleen channel.There is tonifying heart and spleen, nourish blood allay excitement, invigorating the spleen to arrest diarrhea, the effect such as inducing diuresis to reduce edema.Be applicable to the illnesss such as body is after being ill empty, the deficiency of blood is sallow, insufficiency of vital energy and blood, neurasthenia, palpitation and severe palpitation, forgetful insomnia.
Red date is rich in the nutritional labelings such as protein, fat, carbohydrate, carrotene, B family vitamin, vitamin C, citrin and calcium, phosphorus, iron and CAMP.Wherein ascorbic content comes out at the top in fruit, has the laudatory title of vitamin king, has effect of blood-enriching face-nourishing.CAMP contained by red date is the required composition of human body cell energetic supersession, can strengthening muscular strength, dispelling fatigue, hemangiectasis, increase myocardial contractive power, improve myocardial nutrition, having good effect to preventing and treating disease of cardiovascular system; In the traditional Chinese medical science, theory is thought, red date has the effects such as qi-restoratives benefit gas, nourishing blood and tranquilization, strengthening the spleen and stomach, and red date has good therapeutic effect to illnesss such as chronic hepatitis, cirrhosis, anaemia, anaphylactoid purpuras; Red date contains triterpene compound and CAMP, has stronger anticancer, anti-allergic effects.Date can moisten cardiopulmonary, cough-relieving, tonifying five zang organs, it is deficient to control, except stomach addiction gas, support spleen, flat stomach Qi, logical nine orifices, help 12 warps in can also pacifying.
The fruit of Chinese wolfberry, (FRUCTUSLYCII) calls beet red ear pendant ground frame, is the ripening fruits of matrimony vine of solanaceae plant.Pluck when summer, autumn fruit maturation, removing carpopodium, put after shady and cool place dries in the air and corrugate to pericarp, then be exposed to the sun stiff to crust, pulp is soft and get final product.Meet overcast and rainy available low baking temperature to dry.Having various health care functions, is the medicine-food two-purpose food of Ministry of Public Health's approval.LBP-X, betaine is nourishing liver and kidney, benefiting shrewd head.
Sealwort (formal name used at school: Polygonatumsibiricum), has another name called: polygonatum sibiricum Redoute, yellow chicken dish, shaft of a writing brush dish, claw ginseng, tendrilleaf solomonseal rhizome, shank are joined.For HUANGJING ZANYU CAPSULE, rhizome horizontal walk, cylindric, tubercle expands.Impeller is raw, stockless.Medicinal plant, has tonifying spleen, the effect that moistening lung is promoted the production of body fluid.
Along with the quickening of socioeconomic prosperity, rhythm of life, the day by day fierce of competition, modern particularly a middle-aged person will bear the dual heavy burden of family and work, the factor affecting health has a very large change, and the muscle power of long-term excess load, mental labour cause body's immunity decline to such an extent as to cause various diseases.
Modern medicine, cell biology and molecular biological development, people are recognized, and immune disorder not only can produce various diseases, and all have substantial connection with the generation of frequently-occurring disease as tumour, hypertension, diabetes of human senility and the elderly, immunologic function has age_related changes, and namely the age increases, and its immunologic function declines or disorder, result atrophy of thymus gland, T cell loss, thus cause body aging, the lost of life.And Chinese medicine immunomodulator can strengthen the immunologic function of the elderly, closely during the last ten years, chemists obtain multiple compounds from the help class Chinese medicine such as to strengthen the body resistance to consolidate the constitution, and not only can promote the immunologic function of body, and confirm really have very strong immunocompetence.Therefore find and there is good immunoregulation effect, with the medicine of the various diseases such as prevention and therapy tumour, cardiovascular and cerebrovascular.In order to reach daily health-care effect, be badly in need of a kind of instant and the food of safe and reliable integration of drinking and medicinal herbs.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of cordyceps drink, be primary raw material with Cordyceps militaris, be equipped with arillus longan, date, the fruit of Chinese wolfberry, sealwort, the famous and precious genuine integration of drinking and medicinal herbs Chinese medicine of Radix Glycyrrhizae multi-flavor, develop New-generation healthy beverage---Worm grass oral.In side, the fruit of Chinese wolfberry, arillus longan, date, sealwort are all help class Chinese medicine, and to improving human-body sub-health, improving immunity of organisms and having certain effect, Radix Glycyrrhizae works to be in harmonious proportion the property of medicine.
Present invention also offers the preparation method of cordyceps drink, the method is acted on the traditional draft of China and is soaked fresh-keeping extraction process, and in conjunction with the meticulous processing of modern film concentration technique, mouthfeel is excellent, and applicable masses drink.
The present invention is achieved through the following technical solutions:
A kind of cordyceps drink, it is made up of following materials by weight percentage:
Sweetener 3%-9%
Cordyceps militaris 0.01%-0.04%
Arillus longan 0.01%-0.03%
Date 0.01%-0.03%
Fruit of Chinese wolfberry 0.01%-0.02%
Sealwort 0.01%-0.02%
Radix Glycyrrhizae 0.005%-0.015%, surplus is water.
Preferred a kind of cordyceps drink, it is made up of following materials by weight percentage:
Sweetener 4%-8%
Cordyceps militaris 0.02%-0.04%
Arillus longan 0.01%-0.02%
Date 0.01%-0.03%
Fruit of Chinese wolfberry 0.01%-0.02%
Sealwort 0.01%-0.02%
Radix Glycyrrhizae 0.005%-0.015%, surplus is water.
Best a kind of cordyceps drink, it is made up of the raw material of following weight percentage:
Sweetener 7%
Cordyceps militaris 0.035%
Arillus longan 0.02%
Date 0.02%
The fruit of Chinese wolfberry 0.01%
Sealwort 0.01%
Radix Glycyrrhizae 0.010%, surplus is water.
Present invention also offers a kind of preparation method of cordyceps drink, comprise the steps
(1) percentage by weight raw material is got: sweetener 4%-8%, Cordyceps militaris: 0.02%-0.04%, arillus longan 0.01%-0.02%, date 0.01%-0.03%, fruit of Chinese wolfberry 0.01%-0.02%, sealwort 0.01%-0.02%, Radix Glycyrrhizae 0.005%-0.015%, water are for subsequent use;
(2) medicinal material inspissated juice extracts: get Cordyceps militaris, arillus longan, date, the fruit of Chinese wolfberry, sealwort, licorice medicinal materials add water boil, obtain extract I filter after temporary; Add boiling water in the dregs of a decoction after first time extraction, boil, extract II, merge extract I and II, be cooled to below room temperature, cooling fluid is centrifugal, and extracting centrifugal liquid concentrates, and obtains inspissated juice;
(3) product preparing: get inspissated juice 12-20%, heating water fully dissolves; Get sweetener, be dissolved in water into liquid glucose, inspissated juice lysate and liquid glucose are put in blend tank, allocate.
Preferred preparation method, comprises the steps: further
(1) get percentage by weight raw material: sweetener 4%-8%, Cordyceps militaris 0.02%-0.04%, arillus longan 0.01%-0.02%, date 0.01%-0.03%, fruit of Chinese wolfberry 0.01%-0.02%, sealwort 0.01%-0.02%, Radix Glycyrrhizae 0.005%-0.015%, water is for subsequent use;
(2) medicinal material inspissated juice extracts: get Cordyceps militaris, arillus longan, date, the fruit of Chinese wolfberry, sealwort, licorice medicinal materials add 10-15 times of water boil, keep micro-boiling to extract 0.5-3 hour, obtain extract I filter after temporary; 8-12 times of boiling water is added in the dregs of a decoction after first time extraction, boil, keep micro-0.5-3 hour that boils, extract II, merge extract I and II, be cooled to below room temperature, cooling fluid is centrifugal, and extracting centrifugal liquid is crossed film and concentrated, be concentrated into relative density for (more than 15%), obtain inspissated juice, inspissated juice sterilization, filling;
(3) product preparing: get inspissated juice 14-18%, heating water fully dissolves; Get sweetener, be dissolved in water into 30-80% liquid glucose, inspissated juice lysate and liquid glucose are put in blend tank, add water to scale.
Further preferably preparation method, comprises the steps: again
(1) get percentage by weight raw material: sweetener 7%, Cordyceps militaris 0.035%, arillus longan 0.02%, date 0.02%, the fruit of Chinese wolfberry 0.01%, sealwort 0.01%, Radix Glycyrrhizae 0.010%, water is for subsequent use;
(2) get Cordyceps militaris, arillus longan, date, the fruit of Chinese wolfberry, sealwort, licorice medicinal materials add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merge extract I and II, be cooled to less than 20 DEG C, cooling fluid centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, being concentrated into relative density for (more than 15%) namely obtains inspissated juice, inspissated juice at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C;
(3) product preparing: get inspissated juice 16%, heating water fully dissolves; Get sweetener, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to scale.
Product pop can, PET bottle or Tetra Pak that method of the present invention also comprises step (4) deployed are filling, obtain finished product after the assay was approved.
Film described in step of the present invention (2) is reverse osmosis membrane, and membrane aperture requires as only allowing hydrone to pass through.By membrane filtration, retain the fragrance component of plant beverage to greatest extent, be better than the plant beverage produced by traditional handicraft with the plant beverage local flavor of this explained hereafter.
Centrifugal employing disk centrifugal separator described in step of the present invention (2), 4000-6000r/min.
Cordyceps drink of the present invention also can be solid beverage.
Sweetener of the present invention be white granulated sugar, soft white sugar, sucrose, glucose, saccharin sodium, HFCS, honey, sorbierite, sweet mellow wine, honey element, Aspartame, steviol glycoside, alitame, Radix Glycyrrhizae, antierythrite, xylitol, as in sugar alcohol, maltitol, D-mannital one or more.Preferred sweeteners be in white granulated sugar, soft white sugar, sucrose one or more.
Water of the present invention is process water.
Beneficial effect
Beneficial effect of the present invention is set forth by following test example
1 materials and methods
1.1 samples: Worm grass oral (batching of embodiment and preparation), 250ml/ tank, canned, liquid, normal temperature is preserved, and human body recommended daily dosage is 2 tanks/sky, and in the body weight 60kg that is grown up, recommended amounts is 8.33ml/kgBW.The concentrating sample (cycles of concentration is 33.9 times) that this test adopts producer to provide is tested.
1.2 animals used as test and laboratory: the female small white mouse of SPF level Kunming kind, 6 ~ 8 week age (body weight: 18g ~ 22g), thered is provided by Guangdong Medical Lab Animal Center, the quality certification number: SCXK (Guangdong) 2013 ?0002, the certification of fitness is numbered: 44007200021570, and pellet is also provided by Guangdong Medical Lab Animal Center; Animal Lab. is barrier environment, occupancy permit number: SYXK (Guangdong) 2013 ?0011, room temperature 20 ~ 26 DEG C, humidity 40 ~ 70%.
1.3 dosage choice and tested material process and give mode
1.3.1 dosage choice: basic, normal, high three dosage groups are established in this experiment, dosage is 41.7ml/kgBW, 83.3ml/kgBW, 250.0ml/kgBW, conversion is the dosage of concentrating sample is 1.23ml/kgBW, 2.46ml/kgBW, 7.37ml/kgBW, be equivalent to 5,10,30 times of human body recommended amounts, separately establish negative control group.
1.3.2 tested material process and give mode: measure concentrating sample 73.7ml during experiment, be mixed with the sample liquid of 200.0ml with pure water, for high dose group animal gavage; From high dose group sample liquid, get 50.0ml, be diluted to 150.0ml with pure water, in dosage treated animal gavage; Therefrom get 50.0ml in dosage group sample liquid, be diluted to 100.0ml with pure water, for low dose group animal gavage.Every day measures gavage animal by 0.2ml/10gBW and gives tested material.
1.4 key instruments and reagent: CO 2incubator, dT type cell counter, SOFTmaxPRO ELIASA, superclean bench, spectrophotometer, inverted microscope, microscope, incubator, slide measure, RPMI1640 culture medium, sheep red blood cell (SRBC), MTT and ConA etc.
1.5 test methods: after animal is quarantined three days in laboratory conditions, are divided into I, II, III, IV batch by animal, often criticize 40 animals and be divided into four groups at random, often organize 10, every day, gavage gave tested material, and to sample amount according to body weight increase and decrease adjustment weekly, experimental period is 4 weeks.
1.5.1 mouse delayed allergy test (sufficient sole of the foot thickness increases method) (the Ith batch of animal)
The 4th day immune animal before experiment terminates, by 2% (v/v) sheep red blood cell (SRBC) lumbar injection 0.2ml sensitized animal, left back sufficient sole of the foot portion thickness is measured after 5 days, then in this place's hypodermic injection 20% (v/v) sheep red blood cell (SRBC) (the every mouse of 20 μ l/), inject and measure left back sufficient sole of the foot portion thickness three times in latter 24 hours, obtain mean value.
The mouse spleen lymphocyte conversion test (mtt assay) (the IIth batch of animal) of 1.5.2ConA inducing
Prepared by splenocyte suspension: asepticly get spleen, is placed in the little plate filling appropriate aseptic Hank ' s liquid, is torn up by spleen gently, make individual cells suspension with embedding son.Filter through 200 eye mesh screens, Hank ' s liquid washes 3 times, each centrifugal 10min (1000r/min).Then cell is suspended in the complete culture solution of 2ml, uses dT cell counter counting splenocyte, adjustment cell concentration is 3 × 10 6individual/ml.
Lymphproliferation response: add in 24 well culture plates by a cell suspension point holes, every hole 1ml, a hole adds 75 μ lConA liquid (being equivalent to 7.5ug/ml), and 5%CO in contrast, is put in another hole 2, cultivate 72h for 37 DEG C.Cultivation terminates first 4 hours, and every hole sucks supernatant 0.7ml, adds 0.7ml serum-free RPMI1640 nutrient solution, adds MTT (5mg/ml) 50 μ l/ hole simultaneously, continues to cultivate 4h.After cultivation terminates, every hole adds 1ml acid isopropyl alcohol, and piping and druming mixing, makes purple crystal dissolve, and divides and is added in 96 well culture plates, makes the Duplicate Samples in 2 holes, measures OD value with 570nm wavelength.
1.5.3 mice serum hemolysin titer determination (HD50 value method) (the Ith batch of animal)
The 4th day immune animal before experiment terminates, the sheep red blood cell (SRBC) 0.2ml of every lumbar injection 2%, within 5 days, extract eyeball blood sampling afterwards, separation of serum is for subsequent use.Hemolytic reaction: with SA buffer solution by serum-dilution 100 times, the serum 1ml after dilution is put in vitro, adds 10%SRBC0.5ml successively, complement 1ml (pressing 1:8 dilution with SA liquid), separately establishes the control tube of not increase serum; Put 30min in 37 DEG C of incubators, ice bath cessation reaction, centrifugal (2000r/min) 10min, gets supernatant 1ml, adds Dou Shi reagent 3ml, gets 10%SRBC0.25ml simultaneously and adds Dou Shi reagent to 4ml, fully mix; After placing 10min, measure each pipe OD value respectively in 540nm place.
1.5.4 mouse boosting cell antibody tormation test (the IIth batch of animal)
Immune animal: the sheep blood getting defiber, with brine 3 times, centrifugal (2000r/min) 10min, hematocrit SRBC physiological saline is made into the cell suspension of 2% (v/v) at every turn, every mouse lumbar injection 0.2ml.
Prepared by splenocyte suspension: put to death by the mouse cervical dislocation of SRBC immunity after 4 days, take out spleen, be placed on fill Hank ' s liquid little plate in, grind spleen gently, make cell suspension, filter through 200 eye mesh screens, centrifugal (1000r/min) 10min, wash 2 times with Hank ' s liquid, finally cell is suspended in 5mlRPMI1640 nutrient solution, use dT cell counter counting splenocyte, and cell concentration is adjusted to 5 × 10 6individual/ml.
The mensuration of plaque: after the culture medium heating for dissolving of top layer, put 45 ~ 50 DEG C of water bath heat preservations, with equivalent pH7.2 ~ 7.4, Hank ' the s liquid mixing of 2 times of concentration, packing small test tube, often pipe 0.5ml, 50 μ l10%SRBC (v/v are added again in pipe, use SA buffer), 25 μ l splenocyte suspensions, rapid mixing, be poured on the slide of own brush agarose thin layer, do parallel plate, after agar solidification, slide level being buckled is placed on horse, put into CO2gas incubator and hatch 1 ~ 1.5h, then join in slide frame groove with the complement (1:8) of SA buffer solution dilution, after continuing incubation 1 ~ 1.5h, counting hemolysis plaque number.
1.5.5 mouse carbonic clearance test (the IIIth batch of animal)
With the india ink of 1:4 dilution after last administration, by every mouse 10g body weight 0.1ml tail vein injection, timing immediately, blood 20 μ l is got from angular vein clump in 2,10 minutes respectively in interval, adds 2mlNa 2cO 3in solution, survey OD value with spectrophotometer in 600nm wavelength place, use Na 2cO 3solution does blank, puts to death mouse, gets liver, spleen is weighed, and calculates phagocytic index.
1.5.6 Turnover of Mouse Peritoneal Macrophages engulfs chicken red blood cell test (the IVth batch of animal)
The activation of mouse macrophage: test and inject 2% hematocrit sheeps blood erythrocyte 0.2ml to every mouse peritoneal in first 4 days.Put to death mouse with cervical dislocation, lumbar injection only adds Hank ' the s liquid 4ml/ of calf serum, rubs belly gently 20 times, fully to wash out peritoneal macrophage, then stomach wall is cut off an osculum, draws abdominal cavity washing lotion 2ml in vitro with glue head straw.Add with 1ml sample injector absorption abdominal cavity washing lotion 0.5ml and fill 0.5ml1% chicken red blood cells suspension in vitro, mixing.Draw 0.5ml mixed liquor with syringe, add in the agar circle of slide.15 ~ 20 minutes are hatched for 37 DEG C in placement incubator.Hatch rapid physiological saline after terminating to be washed out by non-attached cell, in methanol solution, fix 1 minute, Giemsa liquid dyes 15 minutes.Clean with distilled water flushing, dry, by 40 × microscopic counting phagocytic rate and phagocytic index.Phagocytic rate is in every 100 macrophages, engulfs the percentage shared by macrophage of chicken red blood cell: phagocytic index is the number of average each macrophage phagocytic chicken red blood cell.
1.5.7NK cytoactive detection (LDH method) (the IIth batch of animal)
The preparation (effector cell) of splenocyte suspension: asepticly get spleen, is placed in the little plate filling appropriate aseptic Hank ' s liquid, is torn up by spleen gently, make single cell suspension with tweezers.Filter through 200 eye mesh screens, Hank ' s liquid washes 3 times, each centrifugal 10min (1000r/min).Then cell is suspended in the complete culture solution of 2ml, uses dT cell counter counting splenocyte, finally adjusting cell concentration with RPMI1640 complete culture solution is 2 × 10 7individual/ml.
NK cytoactive detects: getting concentration is 4 × 10 5the YAC of individual/ml ?1 target cell and each 100 μ l of effector cell (effect target is than 50:1), add U-shaped 96 well culture plates; Target cell Spontaneous release hole adds target cell and each 100 μ l of nutrient solution, and the maximum release aperture of target cell adds target cell and each 100 μ l of 1%NP40; Above-mentionedly everyly all establish three multiple holes, in 37 DEG C, 5%CO 2cultivate 4h in incubator, then by 96 well culture plates with the centrifugal 5min of 1500r/min, every hole is drawn in 96 well culture plates at the bottom of supernatant 100 μ l horizontalization, add LDH matrix liquid 100 μ l simultaneously, reaction 3min, every hole adds 1mol/LHCl30 μ l, and at enzyme connection, instrument 492nm place measures OD value.
1.6 test data statistics: adopt variance analysis, first carry out homogeneity test of variance by the program of variance analysis, variance is neat, calculates F value, F value <F 0.05, conclusion: no significant difference between each group mean; F value>=F 0.05, P≤0.05, adds up with the comparative approach between two of mean between multiple experimental group and a control group; Suitable variable transitions is carried out to the data of abnormal or heterogeneity of variance, after meeting normal state or variance and requiring together, adds up by the data after conversion; If do not reach normal state or the neat object of variance after variable transitions yet, use rank test instead and add up.
1.7 results judge
Develop immunitypty function judges: in cellular immune function, humoral immune function, monokaryon-macrophage function, NK cytoactive four, result is positive in any two, can judge this given the test agent develop immunitypty function.
1.7.1 cellular immune function result judges: two experimental results in cellular immune function assay project are the positive, or two dosage group results of any one experiment are positive, can judge that cellular immune function assay result is positive.
1.7.2 humoral immune function result judges: two experimental results in humoral immune function mensuration project are the positive, or two dosage group results of any one experiment are positive, can judge that humoral immune function measurement result is positive.
1.7.3 monokaryon-macrophage function result judges: two experimental results in monokaryon-macrophage function mensuration project are the positive, or two dosage group results of any one experiment are positive, can judge that monokaryon-macrophage function result is positive.
1.7.4NK cytoactive result judges: the above result of a dosage group of NK cytoactive detection experiment is positive, can judge that NK cytoactive result is positive.
2 results
2.1 Worm grass orals are on the impact of Mouse Weight: serum hemolysin and delayed allergy group are in table 1, and lymphocyte transformation, NK determination of activity, antibody tormation group are in table 2, and carbonic clearance experimental group, in table 3, engulfs chicken red blood cell group in table 4.Comparatively, difference there are no significant meaning, shows that the body weight growth of Worm grass oral to mouse has no significant effect to each dosage group each phase body weight weight ratio in period corresponding to control group.
Table 1. Worm grass oral on the impact of Mouse Weight (the Ith batch of animal, n=10, )
Table 2. Worm grass oral on the impact of Mouse Weight (the IIth batch of animal, n=10, )
Table 3. Worm grass oral on the impact of Mouse Weight (the IIIth batch of animal, n=10, )
Table 4. Worm grass oral on the impact of Mouse Weight (the IVth batch of animal, n=10, )
2.2 Worm grass orals are on the impact of mouse spleen weight and thymic weight: each dosage group internal organs/body weight ratio compares with control group, difference there are no significant meaning (see table 5).Show that Worm grass oral has no significant effect the spleen weight of mouse and thymic weight.
Table 5. Worm grass oral on the impact of mouse thymus, spleen weight (n=10, )
2.3 Worm grass orals are on the impact of the delayed allergy of mouse: the middle and high dosage group foot sole of the foot thickens value higher than control group, and difference has significant (see table 6).Show Worm grass oral to the delayed allergy result of the test of mouse for positive.
Table 6. Worm grass oral is on the impact of mouse delayed allergy
2.4 Worm grass orals are on the impact of the mouse spleen lymphocyte conversion reaction that ConA induces: each dosage group optical density difference compares with control group, difference there are no significant meaning (see table 7).Show Worm grass oral to mouse spleen lymphocyte conversion test result for negative.
Table 7. Worm grass oral is on the impact of mouse spleen lymphocyte conversion reaction
2.5 Worm grass orals are on the impact of animal subject serum hemolysin HD50 value: the serum hemolysin HD50 value of basic, normal, high dosage group is all higher than control group, and difference has significant (see table 8).Show Worm grass oral to animal subject serolysin test result for positive.
Table 8. Worm grass oral is on the impact of mice serum hemolysin HD50 value
2.6 Worm grass orals are on the impact of animal subject splenocyte antibody tormation level: the splenocyte antibody tormation level of each dosage group compares with control group, difference there are no significant meaning (see table 9).Show Worm grass oral to animal subject splenocyte antibody tormation level determination result for negative.
Table 9. Worm grass oral is on the impact of mouse boosting cell antibody tormation level
2.7 Worm grass orals are on the impact of mouse carbonic clearance phagocytic function: each dosage group carbonic clearance phagocytic index compares with control group, difference there are no significant meaning (see table 10).Show that the carbonic clearance phagocytosis result of the test of Worm grass oral is for negative.
Table 10. Worm grass oral is on the impact of mouse carbonic clearance phagocytic function
2.8 Worm grass orals engulf the impact of chicken red blood cell phagocytic function to Turnover of Mouse Peritoneal Macrophages: phagocytic percentage conversion value and the phagocytic index of each dosage group compare with control group, difference there are no significant meaning (see table 11).Show that the Turnover of Mouse Peritoneal Macrophages of Worm grass oral engulfs chicken red blood cell result of the test for negative.
Table 11. Worm grass oral on Turnover of Mouse Peritoneal Macrophages engulf chicken red blood cell phagocytic function impact (n=10, )
Phagocytic percentage conversion value: X=Sin ?1(p) 1/2
2.9 Worm grass orals are on the impact of animal subject NK cytoactive: the NK cytoactive of each dosage group compares with control group, difference there are no significant meaning (see table 12).Show Worm grass oral to animal subject NK cell activity assays result for negative.
Table 12. Worm grass oral on the impact of NK cells in mice activity (n=10, )
NK cytoactive conversion value: X=Sin ?1(p) 1/2
3 brief summaries
To SPF level Kunming mouse per os to give respectively every day Worm grass oral concentrating sample (cycles of concentration is 33.9 times) 1.23,2.46,7.37ml/kgBW, be equivalent to Worm grass oral 41.7ml/kgBW, 83.3ml/kgBW, 250.0ml/kgBW, for 5,10,30 times of Worm grass oral recommended daily dosage, totally 4 weeks, result shows: the mouse delayed allergy result of the test of sheep red blood cell (SRBC) induction is positive, and given the test agent has the effect of enhancing cellular immune function; After sheep red blood cell (SRBC) immunization of animal subjects, serolysin test result is positive, and given the test agent has enhancing body fluid immunity function; Carbonic clearance test and macrophage phagocytic chicken red blood cell result of the test negative, given the test agent do not have enhancing Dan He ?macrophage function effect; NK cell activity assays is negative, and given the test agent does not have the effect of enhancing NK cytoactive; The mouse spleen lymphocyte proliferation result of the test of ConA induction is negative; After sheep red blood cell (SRBC) immunization of animal subjects, splenocyte antibody tormation result of the test is negative.
Judge according to " health food inspection and assessment technical specification " develop immunitypty functional evaluation standard, Worm grass oral has develop immunitypty function
The cordyceps drink prepared by formula of the present invention and technique keeps lower apparent viscosity, thus make delicate mouthfeel, mellow, tasty and refreshing plant beverage, has brand-new peculiar flavour, good mouthfeel, not only meet the standard of national tea beverage, also there is certain health-care effect, to improving human-body sub-health, improving immunity of organisms and there is certain effect.
Composition in right of the present invention and the composition of embodiment according to actual production, can zoom in or out ratio simultaneously.
Detailed description of the invention
Detailed description of the invention is only used to content of the present invention is described, is not the further restriction to claims of the present invention.
Embodiment 1
Get Cordyceps militaris 0.35kg, arillus longan 0.2kg, date 0.2kg, fruit of Chinese wolfberry 0.1kg, sealwort 0.1kg, Radix Glycyrrhizae 0.1kg medicinal material add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 16kg, heating water fully dissolves; Get white granulated sugar 70kg, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 2
Get Cordyceps militaris 0.35kg, arillus longan 0.2kg, date 0.2kg, fruit of Chinese wolfberry 0.1kg, sealwort 0.1kg, Radix Glycyrrhizae 0.1kg medicinal material add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 14kg, heating water fully dissolves; Get white granulated sugar 90kg, be dissolved in water into 80% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 3
Get Cordyceps militaris 0.4kg, arillus longan 0.3kg, date 0.3kg, fruit of Chinese wolfberry 0.2kg, sealwort 0.2kg, Radix Glycyrrhizae 0.15kg medicinal material add 15 times of water boils, keep micro-extraction 0.5 hour of boiling, obtain extract I filter after temporary; Add 12 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 18kg, heating water fully dissolves; Get white granulated sugar 80kg, be dissolved in water into 70% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 4
Get Cordyceps militaris 0.2kg, arillus longan 0.1kg, date 0.1kg, fruit of Chinese wolfberry 0.1kg, sealwort 0.1kg, Radix Glycyrrhizae 0.05kg medicinal material add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 16kg, heating water fully dissolves; Get white granulated sugar 40kg, be dissolved in water into 30% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 5
Get Cordyceps militaris 0.4kg, arillus longan 0.2kg, date 0.3kg, fruit of Chinese wolfberry 0.2kg, sealwort 0.2kg, Radix Glycyrrhizae 0.15kg medicinal material add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 16kg, heating water fully dissolves; Get white granulated sugar 70kg, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 6
Get Cordyceps militaris 0.35kg, arillus longan 0.2kg, date 0.2kg, fruit of Chinese wolfberry 0.1kg, sealwort 0.1kg, Radix Glycyrrhizae 0.1kg medicinal material add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 16kg, heating water fully dissolves; Get maltose 70kg, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 7
Get Cordyceps militaris 0.35kg, arillus longan 0.2kg, date 0.2kg, fruit of Chinese wolfberry 0.1kg, sealwort 0.1kg, Radix Glycyrrhizae 0.1kg medicinal material add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 16kg, heating water fully dissolves; Get glucose 70kg, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 8
Get Cordyceps militaris 0.1kg, arillus longan 0.1kg, date 0.1kg, fruit of Chinese wolfberry 0.1kg, sealwort 0.1kg, Radix Glycyrrhizae 0.05kg medicinal material add 8 times of water boils, keep micro-extraction 3 hours of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 16kg, heating water fully dissolves; Get white granulated sugar 70kg, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 9
Get Cordyceps militaris 0.35kg, arillus longan 0.2kg, date 0.2kg, fruit of Chinese wolfberry 0.1kg, sealwort 0.1kg, Radix Glycyrrhizae 0.1kg medicinal material add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 12kg, heating water fully dissolves; Get soft white sugar 70kg, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.
Embodiment 10
Get Cordyceps militaris 0.35kg, arillus longan 0.2kg, date 0.2kg, fruit of Chinese wolfberry 0.1kg, sealwort 0.1kg, Radix Glycyrrhizae 0.1kg medicinal material add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merges extract I and II, is cooled to less than 20 DEG C, cooling fluid disk centrifugal (4000-6000r/min), extracting centrifugal liquid is crossed film and is concentrated, and be concentrated into relative density and namely obtain inspissated juice for (more than 15%), inspissated juice is at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C: get inspissated juice 20kg, heating water fully dissolves; Get sucrose 70kg, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to 1 ton, filling with pop can, PET bottle or Tetra Pak, obtains finished product after the assay was approved.

Claims (10)

1. a cordyceps drink, it is made up of following materials by weight percentage:
Sweetener 3%-9%
Cordyceps militaris 0.01%-0.04%
Arillus longan 0.01%-0.03%
Date 0.01%-0.03%
Fruit of Chinese wolfberry 0.01%-0.02%
Sealwort 0.01%-0.02%
Radix Glycyrrhizae 0.005%-0.015%, surplus is water.
2. a kind of cordyceps drink as claimed in claim 1, it is made up of following materials by weight percentage:
Sweetener 4%-8%
Cordyceps militaris 0.02%-0.04%
Arillus longan 0.01%-0.02%
Date 0.01%-0.03%
Fruit of Chinese wolfberry 0.01%-0.02%
Sealwort 0.01%-0.02%
Radix Glycyrrhizae 0.005%-0.015%, surplus is water.
3. cordyceps drink as claimed in claim 2, it is made up of the raw material of following weight percentage:
Sweetener 7%
Cordyceps militaris 0.035%
Arillus longan 0.02%
Date 0.02%
The fruit of Chinese wolfberry 0.01%
Sealwort 0.01%
Radix Glycyrrhizae 0.010%, surplus is water.
4. the cordyceps drink as described in claim 1-3 any one, is characterized in that: described sweetener be white granulated sugar, soft white sugar, sucrose, glucose, saccharin sodium, HFCS, honey, sorbierite, sweet mellow wine, honey element, Aspartame, steviol glycoside, alitame, Radix Glycyrrhizae, antierythrite, xylitol, as sugar alcohol, maltitol, D ?in mannitol one or more; Described sweetener be in white granulated sugar, soft white sugar, sucrose one or more.
5. a preparation method for cordyceps drink, is characterized in that, comprises the steps:
(1) percentage by weight raw material is got: sweetener 4%-8%, Cordyceps militaris: 0.02%-0.04%, arillus longan 0.01%-0.02%,
Date 0.01%-0.03%, fruit of Chinese wolfberry 0.01%-0.02%, sealwort 0.01%-0.02%, Radix Glycyrrhizae 0.005%-0.015%, water are for subsequent use;
(2) medicinal material inspissated juice extracts: get Cordyceps militaris, arillus longan, date, the fruit of Chinese wolfberry, sealwort, licorice medicinal materials add water boil, obtain extract I filter after temporary; Add boiling water in the dregs of a decoction after first time extraction, boil, extract II, merge extract I and II, be cooled to below room temperature, cooling fluid is centrifugal, and extracting centrifugal liquid concentrates, and obtains inspissated juice;
(3) product preparing: get inspissated juice 12-20%, heating water fully dissolves; Get sweetener, be dissolved in water into liquid glucose, inspissated juice lysate and liquid glucose are put in blend tank, allocate.
6. preparation method as claimed in claim 5, is characterized in that, comprise the steps:
(1) percentage by weight raw material is got: sweetener 4%-8%, Cordyceps militaris 0.02%-0.04%, arillus longan 0.01%-0.02%, date 0.01%-0.03%, fruit of Chinese wolfberry 0.01%-0.02%, sealwort 0.01%-0.02%, Radix Glycyrrhizae 0.005%-0.015%, water are for subsequent use;
(2) medicinal material inspissated juice extracts: get Cordyceps militaris, arillus longan, date, the fruit of Chinese wolfberry, sealwort, licorice medicinal materials add 10-15 times of water boil, keep micro-boiling to extract 0.5-3 hour, obtain extract I filter after temporary; Add 8-12 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-0.5-3 hour that boils, extract II, merge extract I and II, be cooled to below room temperature, cooling fluid is centrifugal, and extracting centrifugal liquid is crossed film and concentrated, being concentrated into relative density is more than 15%, obtains inspissated juice, and inspissated juice sterilization is filling;
(3) product preparing: get inspissated juice 14-18%, heating water fully dissolves; Get sweetener, be dissolved in water into 30-80% liquid glucose, inspissated juice lysate and liquid glucose are put in blend tank, add water to scale.
7. preparation method as claimed in claim 6, is characterized in that, comprise the steps:
(1) get percentage by weight raw material: sweetener 7%, Cordyceps militaris 0.035%, arillus longan 0.02%, date 0.02%, the fruit of Chinese wolfberry 0.01%, sealwort 0.01%, Radix Glycyrrhizae 0.010%, water is for subsequent use;
(2) get Cordyceps militaris, arillus longan, date, the fruit of Chinese wolfberry, sealwort, licorice medicinal materials add 12 times of water boils, keep micro-extraction 1 hour of boiling, obtain extract I filter after temporary; Add 10 times of boiling water in the dregs of a decoction after first time extraction, boil, keep micro-and boil 1 hour, extract II, merge extract I and II, be cooled to less than 20 DEG C, cooling fluid is centrifugal, extracting centrifugal liquid is crossed film and is concentrated, being concentrated into relative density is more than 15% namely obtain inspissated juice, inspissated juice at 138 DEG C ± 2 DEG C, 20-36s sterilization, filling, filling liquid temperature≤25 DEG C;
(3) product preparing: get inspissated juice 16%, heating water fully dissolves; Get sweetener, be dissolved in water into 60% liquid glucose, put in blend tank by inspissated juice lysate and liquid glucose, the allotment that adds water is to scale.
8. the preparation method as described in claim 5-7 any one, is characterized in that: the product pop can, PET bottle or the Tetra Pak that comprise step (4) deployed are filling, obtains finished product after the assay was approved.
9. preparation method as claimed in claim 6, is characterized in that: the film described in step (2) is reverse osmosis membrane; Described centrifugal employing disk centrifugal.
10. cordyceps drink according to claim 1 strengthens the application on body immunity health products or food in preparation.
CN201510776271.0A 2014-11-14 2015-11-12 A kind of cordyceps drink and preparation method thereof Active CN105360824B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510776271.0A CN105360824B (en) 2014-11-14 2015-11-12 A kind of cordyceps drink and preparation method thereof

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201410650345 2014-11-14
CN2014106503451 2014-11-14
CN201510776271.0A CN105360824B (en) 2014-11-14 2015-11-12 A kind of cordyceps drink and preparation method thereof

Publications (2)

Publication Number Publication Date
CN105360824A true CN105360824A (en) 2016-03-02
CN105360824B CN105360824B (en) 2018-11-23

Family

ID=55363912

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510776271.0A Active CN105360824B (en) 2014-11-14 2015-11-12 A kind of cordyceps drink and preparation method thereof

Country Status (1)

Country Link
CN (1) CN105360824B (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105747046A (en) * 2016-03-11 2016-07-13 辽宁隆维生物科技有限公司 Cordyceps militaris liquid beverage and preparation method thereof
CN107304398A (en) * 2017-05-09 2017-10-31 张帆 A kind of preparation technology for the Cordyceps militaris magma product for improving immunity
CN108013269A (en) * 2016-11-02 2018-05-11 浙江泛亚生物医药股份有限公司 A kind of cicada fungus siberian solomonseal beverage and preparation method thereof
CN109222082A (en) * 2018-10-20 2019-01-18 广州王老吉药业股份有限公司 A kind of Chinese medicine composition is preparing the purposes in antifatigue food or health care product
CN109463754A (en) * 2018-10-20 2019-03-15 广州王老吉药业股份有限公司 A kind of Chinese medicine composition is preparing the purposes in antioxidant food or health care product
CN109463504A (en) * 2018-12-27 2019-03-15 山西省农业科学院食用菌研究所 A kind of cordyceps flower function tea beverage and preparation method thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101991154A (en) * 2009-08-21 2011-03-30 康美药业股份有限公司 Vegetable beverage formulation and manufacturing method thereof
TW201132290A (en) * 2010-03-24 2011-10-01 Abundant Seedling Biotech Corp Method of manufacturing extraction fluid of Agaricus blazei Murill
CN102524888A (en) * 2012-01-04 2012-07-04 任文武 Method for preparing Cordyceps militaris sugar-free beverage by microwave extraction
CN103462022A (en) * 2013-09-24 2013-12-25 郑剑峰 Cordyceps militaris nourishing paste and preparation method thereof
CN104068443A (en) * 2014-06-10 2014-10-01 南京泽朗农业发展有限公司 Solid beverage and preparation method thereof
CN104397291A (en) * 2014-12-04 2015-03-11 广州王老吉大健康产业有限公司 Preparation method of concentrated juice of herbal tea

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101991154A (en) * 2009-08-21 2011-03-30 康美药业股份有限公司 Vegetable beverage formulation and manufacturing method thereof
TW201132290A (en) * 2010-03-24 2011-10-01 Abundant Seedling Biotech Corp Method of manufacturing extraction fluid of Agaricus blazei Murill
CN102524888A (en) * 2012-01-04 2012-07-04 任文武 Method for preparing Cordyceps militaris sugar-free beverage by microwave extraction
CN103462022A (en) * 2013-09-24 2013-12-25 郑剑峰 Cordyceps militaris nourishing paste and preparation method thereof
CN104068443A (en) * 2014-06-10 2014-10-01 南京泽朗农业发展有限公司 Solid beverage and preparation method thereof
CN104397291A (en) * 2014-12-04 2015-03-11 广州王老吉大健康产业有限公司 Preparation method of concentrated juice of herbal tea

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105747046A (en) * 2016-03-11 2016-07-13 辽宁隆维生物科技有限公司 Cordyceps militaris liquid beverage and preparation method thereof
CN105747046B (en) * 2016-03-11 2019-05-07 辽宁隆维生物科技有限公司 A kind of Cordyceps militaris liquid drink and preparation method thereof
CN108013269A (en) * 2016-11-02 2018-05-11 浙江泛亚生物医药股份有限公司 A kind of cicada fungus siberian solomonseal beverage and preparation method thereof
CN107304398A (en) * 2017-05-09 2017-10-31 张帆 A kind of preparation technology for the Cordyceps militaris magma product for improving immunity
CN109222082A (en) * 2018-10-20 2019-01-18 广州王老吉药业股份有限公司 A kind of Chinese medicine composition is preparing the purposes in antifatigue food or health care product
CN109463754A (en) * 2018-10-20 2019-03-15 广州王老吉药业股份有限公司 A kind of Chinese medicine composition is preparing the purposes in antioxidant food or health care product
CN109463504A (en) * 2018-12-27 2019-03-15 山西省农业科学院食用菌研究所 A kind of cordyceps flower function tea beverage and preparation method thereof

Also Published As

Publication number Publication date
CN105360824B (en) 2018-11-23

Similar Documents

Publication Publication Date Title
CN103039671B (en) Chinese caterpillar fungus instant tea
CN105360824B (en) A kind of cordyceps drink and preparation method thereof
CN102827750A (en) Maca health care liquor for improving organism immunity and sexuality
CN107581619A (en) A kind of antifatigue anti senility sea cucumber oyster peptide complex capsule and preparation method thereof
CN106244371B (en) A kind of hypoglycemic rhizoma polygonati grape health care wine and its production technology
CN104041903A (en) Selenium-rich cyclocarya paliurus beverage and preparation method thereof
CN107348090B (en) Ginseng candy capable of preserving health and tonifying kidney and preparation method thereof
CN101856120A (en) Health food for improving immunity of human body and preparation method thereof
CN102424625A (en) Edible and medical fungi blackening agent, its preparation method and application
CN102697047A (en) Method for preparing oral liquid of Chinese wolfberry
CN104336174A (en) Rhodiola rosea and milk vinegar contained green tea health beverage
CN104388236B (en) A kind of grape wine and preparation method and application
CN109528814A (en) A kind of probiotics and its preparation method and application of lactobacillus-fermented Radix Astragali
CN108041391A (en) A kind of Black people join theanine drink and preparation method thereof
CN107647395A (en) It is a kind of that there is health food for improving immunity function and preparation method thereof
CN101574151A (en) Nutritional food with function of strengthening immunity and preparation method thereof
CN104928140A (en) Compound fermentation type winter-jujube health care wine and production technology thereof
CN107432911B (en) Ginseng buccal tablet for preserving health and tonifying kidney and preparation method thereof
CN103082240B (en) Carrot-ginseng processing method
CN102229877B (en) Brewing method for pine pollen wine
CN104312875A (en) Preparation method of millet tonic wine
CN107223966B (en) Roxburgh rose and licorice oral liquid for improving immunity and preparation method thereof
CN109486613A (en) One kind is for health liquor and its beverage and preparation method
CN105145931A (en) Hypoglycemic tea and making method thereof
CN104222278A (en) Milk vinegar and green tea health drink with weight-losing function

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant