CN108553559B - Formula and preparation method of vegetable and fruit extract for preventing and treating type II diabetes - Google Patents
Formula and preparation method of vegetable and fruit extract for preventing and treating type II diabetes Download PDFInfo
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Abstract
The invention discloses a formula of a vegetable and fruit extract for preventing and treating type II diabetes and a preparation method thereof, wherein the formula comprises the following raw material components in parts by weight: 5-30 parts of kalanchoe, 4-20 parts of konjak, 3-15 parts of sea-buckthorn, 2-10 parts of common clubmoss herb, 2-10 parts of kudzuvine root and 2-10 parts of momordica grosvenori. A preparation method of vegetable and fruit plant extract for preventing and treating type II diabetes comprises the following steps: s1, washing the pecan, the konjak, the sea-buckthorn, the kudzu-vine root, the momordica grosvenori and the dehulled common macrocarpium fruit, and drying at low temperature for later use; s2, smashing the longevous vegetable, sieving the smashed longevous vegetable with a sieve of 80-100 meshes, using 45% ethanol as a solvent, and enabling the solid-to-liquid ratio to be 1: 10, fully stirring. Compared with the prior art, the vegetable and fruit plant extract composition formula is applied to the field of chronic blood sugar metabolic diseases, is prepared by combining pure natural vegetable and fruit plant extracts, has no toxic or side effect, is not added with external antisepsis and pigment chemical reagents, and is used for preventing and treating type II diabetes.
Description
Technical Field
The invention relates to a plant extract for preventing and treating diabetes, in particular to a formula of a vegetable and fruit extract for preventing and treating type II diabetes and a preparation method thereof.
Background
Diabetes is a chronic, multifactorial, metabolic disease characterized by hyperglycemia, and is a group of metabolic diseases characterized by chronic elevated blood glucose levels. Hyperglycemia, which is present over a long period of time in diabetes, can cause multiple system damage, particularly chronic damage to the eyes, kidneys, heart, blood vessels, nerves, dysfunction, and if not adequately controlled, can also cause complications, serious complications including: cardiovascular diseases, renal failure, blindness, neuropathy, diabetic foot (even amputation) and the like, the incidence rate of diabetes is on a continuously rising trend in the global scope, the mortality rate of the diabetes is second to cardiovascular and cerebrovascular diseases and cancers, and the diabetes is called as the third killer of human health.
According to the statistics data of the health department in 2017, at present, in China, type I diabetes patients are close to 6 million people, type II diabetes patients reach 8 million people, and one hundred million diabetes precursor groups exist, the country needs to consume a great amount of special medical assistance funds for type I diabetes severe patients every year, diabetes brings heavy burden to social economy and personal life, and active prevention and treatment of diabetes are urgent.
At present, patients with type II diabetes are treated by oral medicines, and because of the characteristic of long-term administration, chemical medicines inevitably bring certain toxic and side effects to the patients and even some patients cause drug-induced diseases; the chemical medicine can only treat the symptoms, further injure the liver and the kidney while controlling the blood sugar, and accelerate the patients with the type II to step to the complications.
The homology of medicine and food is one of the most valuable contributions to human beings in the original Chinese medicine, the traditional Chinese medicine emphasizes that the medicine therapy is not as good as the food therapy, aiming at the current situation that certain toxic and side effects exist in long-term use of chemical medicines, people aim at the traditional Chinese medicine or food materials with the blood sugar reducing effect, the public is generally limited by the technology that the traditional Chinese medicine or health-care product with the blood sugar reducing effect is weak in blood sugar reducing effect and accompanied with other biological toxicity, the traditional Chinese medicine or health-care product with the traditional Chinese medicine decoction and the decoction type at high temperature damages the biological activity of various traditional Chinese medicines or nutritional ingredients, the effect is difficult to play, the traditional Chinese medicine or health-care product is the root cause that the clinical treatment of diabetes is almost monopolized by western medicines, and the toxic and side effects generated by long-term use of the chemical medicines damage tissues of the liver, the kidney and the like of diabetes patients, so that the, therefore, a formula of vegetable and fruit extracts for preventing and treating type II diabetes and a preparation method thereof are provided.
Disclosure of Invention
The invention aims to solve the defects in the prior art, provides a formula of vegetable and fruit extracts for preventing and treating type II diabetes and a preparation method thereof, and provides a formula of vegetable and fruit plant extracts for preventing and treating type II diabetes, which is applied to the field of chronic blood sugar metabolic diseases, is formed by combining pure natural vegetable and fruit plant extracts, has no toxic or side effect, is not added with external preservative and pigment chemical reagents, and is used for preventing and treating type II diabetes.
In order to achieve the purpose, the invention provides the following technical scheme: a vegetable and fruit composition for preventing and treating type II diabetes is characterized by comprising the following raw material components in parts by weight: 5-30 parts of kalanchoe, 4-20 parts of konjak, 3-15 parts of sea-buckthorn, 2-10 parts of common clubmoss herb, 2-10 parts of kudzu root and 2-10 parts of momordica grosvenori.
Preferably, the preparation method of the vegetable and fruit plant extract for preventing and treating type II diabetes comprises the following steps:
s1, washing the pecan, the konjak, the sea-buckthorn, the kudzu-vine root, the momordica grosvenori and the dehulled common macrocarpium fruit, and drying at low temperature for later use;
s2, smashing the longevous vegetable, sieving the smashed longevous vegetable with a sieve of 80-100 meshes, using 45% ethanol as a solvent, enabling the solid-liquid ratio to be 1: 10, fully and uniformly stirring, standing for 12 hours, extracting for 30 minutes at the temperature of less than or equal to 45 ℃, separating to obtain a longevous vegetable extract, recovering ethanol from filtrate to obtain a residue of the longevous vegetable, and reserving the residue for next extraction;
s3, crushing the hulled common clubmoss herb, sieving the crushed mixture by a sieve of 80-100 meshes, using 50% ethanol as a solvent, wherein the solid-liquid ratio is 1: 10, fully stirring the mixture, standing the mixture for 12 hours, extracting the mixture for 30 minutes at the temperature of less than or equal to 45 ℃, separating the mixture to obtain a common clubmoss herb extract, recovering ethanol from filtrate to obtain a common clubmoss herb residue, and keeping the common clubmoss herb residue for next extraction;
s4, mixing the pecan residues and the pecan residues, using 90% ethanol as a solvent, wherein the solid-liquid ratio is 1: 15, fully stirring, extracting at the temperature of less than or equal to 45 ℃ for 50 minutes, filtering off the ethanol, extracting with n-butanol to obtain n-butanol extract, concentrating the n-butanol extract by a reduced pressure evaporation method to a proper amount of water, washing and discarding, eluting with 30-100% ethanol solution, separating the extract obtained from the eluent by HPLC, and separating to obtain an alkaline substance;
s5, crushing the sea buckthorn, sieving the ground sea buckthorn with a sieve of 80-100 meshes, using 60 percent ethanol as a solvent, fully stirring the mixture with the solid-liquid ratio of 1: 15, standing the mixture for 12 hours, extracting the mixture for 30 minutes at the temperature of less than or equal to 45 ℃, and recovering the ethanol from the filtrate to obtain a sea buckthorn extract;
s6, crushing the kudzuvine roots, sieving the crushed kudzuvine roots with a sieve of 100 meshes and 120 meshes, using water as a solvent, fully stirring the mixture, extracting the mixture for 90 minutes at the temperature of below 45 ℃ and under the vacuum degree of 0.1mmHg to 1.0mmHg under negative pressure, removing impurities from the water extract by using macroporous resin, and concentrating the water extract under reduced pressure to obtain a puerarin extract;
s7, crushing fructus Siraitiae Grosvenorii, sieving with 80-100 mesh sieve, using water as solvent at solid-to-liquid ratio of 1: 15, stirring, extracting at 45 deg.C below and 0.1-1.0 mmHg under negative pressure for 60 min, removing impurities from the water extractive solution with macroporous resin, and concentrating under reduced pressure to obtain fructus Siraitiae Grosvenorii extract;
s8, smashing konjak, sieving with a 80-100-mesh sieve, using 40% ethanol as a solvent, fully stirring, standing for 12 hours, extracting at the temperature of less than or equal to 45 ℃ for 30 minutes, and recovering ethanol from the filtrate to obtain a glucan substance;
s9, mixing all the above materials at a certain ratio, feeding into a vacuum drying machine under reduced pressure with a vacuum degree of 0.1-1.0 mmHg, and drying at a temperature of 45 deg.C or lower to obtain extract powder.
The invention has the technical effects and advantages that:
1. compared with the existing diabetes drugs, the invention has no side effect harm: the existing diabetes drugs are almost all chemical drugs, side effects are harmful to patients greatly, and the components of the formula are all derived from safe and nontoxic vegetable, fruit and food materials.
2. The invention is not simple to grind the main raw materials into powder and mix them evenly, but the main materials needed by the invention are extracted item by advanced technology, the toxic substances are removed, only the useful components are left, then the invention is made by scientific formula.
3. The invention departs from the theory that the traditional Chinese medicine needs to be decocted at high temperature, adopts low-temperature extraction technology in the preparation process, avoids the activity of various nutrient substances from being damaged, and optimizes the bioavailability.
4. The formula is scientific: through repeated tests and verification, the optimal formula is achieved, and the formula is full of clever sweat in the process of intelligence improvement and trial and error of research personnel.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A preparation method of vegetable and fruit plant extract for preventing and treating type II diabetes comprises the following steps:
s1, washing 50 kg of pecan, 40 kg of konjak, 30 kg of sea buckthorn, 20 kg of kudzu root and 20 kg of momordica grosvenori, shelling 20 kg of ficus carica, and drying at low temperature for later use;
s2, smashing the longevous vegetable, sieving the smashed longevous vegetable with a sieve of 80-100 meshes, using 45% ethanol as a solvent, fully stirring the mixture evenly, standing the mixture for 12 hours, extracting the mixture for 30 minutes at the temperature of less than or equal to 45 ℃, separating the mixture to obtain about 5 kilograms of longevous vegetable extract, and recovering ethanol from filtrate to obtain the residue of the longevous vegetable for further extraction and reuse;
s3, crushing the hulled common anemarrhena fruit, sieving the crushed common anemarrhena fruit by a sieve of 80-100 meshes, using 50% ethanol as a solvent, wherein the solid-liquid ratio is 1: 10, fully stirring, standing for 12 hours, extracting for 30 minutes at the temperature of less than or equal to 45 ℃, separating to obtain about 3 kg of common anemarrhena fruit extract, recovering ethanol from filtrate to obtain common anemarrhena fruit residue, and keeping the common anemarrhena fruit residue for next extraction;
s4, mixing the pecan residues and the pecan residues, using 90% ethanol as a solvent, wherein the solid-liquid ratio is 1: 15, fully stirring, extracting at the temperature of less than or equal to 45 ℃ for 50 minutes, filtering off the ethanol, extracting with n-butanol to obtain n-butanol extract, concentrating the n-butanol extract by a reduced pressure evaporation method to a proper amount of water, discarding, eluting with 30-100% ethanol solution, separating the extract obtained from the eluent by HPLC, and separating to obtain about 2 kg of alkali substances;
s5, crushing the sea buckthorn, sieving the crushed sea buckthorn with a sieve of 80-100 meshes, using 60 percent ethanol as a solvent, fully stirring the mixture at a solid-liquid ratio of 1: 15, standing the mixture for 12 hours, extracting the mixture for 30 minutes at the temperature of less than or equal to 45 ℃, and recovering the ethanol from the filtrate to obtain about 4 kg of the sea buckthorn extract;
s6, crushing the kudzuvine roots, sieving the crushed kudzuvine roots with a sieve of 100 meshes and 120 meshes, using water as a solvent, fully stirring the mixture, extracting the mixture for 90 minutes at the temperature below 45 ℃ and under the vacuum degree of 0.1mmHg to 1.0mmHg under negative pressure, removing impurities from the water extract by using macroporous resin, and concentrating the water extract under reduced pressure to obtain about 3 kg of puerarin extract;
s7, crushing fructus momordicae, sieving with a 80-100 mesh sieve, using water as a solvent with a solid-to-liquid ratio of 1: 15, fully stirring, extracting at a temperature below 45 ℃ and a vacuum degree of 0.1mmHg-1.0mmHg under negative pressure for 60 minutes, removing impurities from the water extract by using macroporous resin, and concentrating under reduced pressure to obtain about 3 kg of fructus momordicae extract;
s8, smashing konjac, sieving with a 80-100-mesh sieve, using 40% ethanol as a solvent, fully stirring, standing for 12 hours, extracting at the temperature of less than or equal to 45 ℃ for 30 minutes, and recovering ethanol from the filtrate to obtain about 6 kg of glucan substances;
s9, mixing all the above materials at a ratio of 26 kg, feeding into a vacuum drying machine with a vacuum degree of 0.1-1.0 mmHg, drying at a temperature of 45 deg.C or lower to obtain extract powder at a ratio of 10 kg, and packaging.
Example 2
A preparation method of vegetable and fruit plant extract for preventing and treating type II diabetes comprises the following steps:
s1, washing 50 kg of pecan, 40 kg of konjak, 30 kg of sea buckthorn, 20 kg of kudzu root and 20 kg of momordica grosvenori, shelling 20 kg of ficus carica, and drying at low temperature for later use;
s2, smashing the longevous vegetable, sieving the smashed longevous vegetable with a sieve of 80-100 meshes, using 45% ethanol as a solvent, fully stirring the mixture evenly, standing the mixture for 12 hours, extracting the mixture for 30 minutes at the temperature of less than or equal to 45 ℃, separating the mixture to obtain about 5 kilograms of longevous vegetable extract, and recovering ethanol from filtrate to obtain the residue of the longevous vegetable for further extraction and reuse;
s3, crushing the hulled common anemarrhena fruit, sieving the crushed common anemarrhena fruit by a sieve of 80-100 meshes, using 50% ethanol as a solvent, wherein the solid-liquid ratio is 1: 10, fully stirring, standing for 12 hours, extracting for 30 minutes at the temperature of less than or equal to 45 ℃, separating to obtain about 3 kg of common anemarrhena fruit extract, recovering ethanol from filtrate to obtain common anemarrhena fruit residue, and keeping the common anemarrhena fruit residue for next extraction;
s4, mixing the pecan residues and the pecan residues, using 90% ethanol as a solvent, wherein the solid-liquid ratio is 1: 15, fully stirring, extracting at the temperature of less than or equal to 45 ℃ for 50 minutes, filtering off the ethanol, extracting with n-butanol to obtain n-butanol extract, concentrating the n-butanol extract by a reduced pressure evaporation method to a proper amount of water, discarding, eluting with 30-100% ethanol solution, separating the extract obtained from the eluent by HPLC, and separating to obtain about 2 kg of alkali substances;
s5, crushing the sea buckthorn, sieving the crushed sea buckthorn with a sieve of 80-100 meshes, using 60 percent ethanol as a solvent, fully stirring the mixture at a solid-liquid ratio of 1: 15, standing the mixture for 12 hours, extracting the mixture for 30 minutes at the temperature of less than or equal to 45 ℃, and recovering the ethanol from the filtrate to obtain about 4 kg of the sea buckthorn extract;
s6, crushing the kudzuvine roots, sieving the crushed kudzuvine roots with a sieve of 100 meshes and 120 meshes, using water as a solvent, fully stirring the mixture, extracting the mixture for 90 minutes at the temperature below 45 ℃ and under the vacuum degree of 0.1mmHg to 1.0mmHg under negative pressure, removing impurities from the water extract by using macroporous resin, and concentrating the water extract under reduced pressure to obtain about 3 kg of puerarin extract;
s7, crushing fructus momordicae, sieving with a 80-100 mesh sieve, using water as a solvent with a solid-to-liquid ratio of 1: 15, fully stirring, extracting at a temperature below 45 ℃ and a vacuum degree of 0.1mmHg-1.0mmHg under negative pressure for 60 minutes, removing impurities from the water extract by using macroporous resin, and concentrating under reduced pressure to obtain about 3 kg of fructus momordicae extract;
s8, smashing konjac, sieving with a 80-100-mesh sieve, using 40% ethanol as a solvent, fully stirring, standing for 12 hours, extracting at the temperature of less than or equal to 45 ℃ for 30 minutes, and recovering ethanol from the filtrate to obtain about 6 kg of glucan substances;
s9, mixing about 26 kg of the above substances, adding 1 kg of modified corn starch as an auxiliary material, fully and uniformly granulating by a granulator, feeding into a vacuum drier after granulation, wherein the vacuum degree is 0.1-1.0 mmHg, drying to obtain about 11 kg of granules, and packaging to obtain the finished product.
Example 3
A preparation method of vegetable and fruit plant extract for preventing and treating type II diabetes comprises the following steps:
s1, washing 50 kg of pecan, 40 kg of konjak, 30 kg of sea buckthorn, 20 kg of kudzu root and 20 kg of momordica grosvenori, shelling 20 kg of ficus carica, and drying at low temperature for later use;
s2, smashing the longevous vegetable, sieving the smashed longevous vegetable with a sieve of 80-100 meshes, using 45% ethanol as a solvent, fully stirring the mixture evenly, standing the mixture for 12 hours, extracting the mixture for 30 minutes at the temperature of less than or equal to 45 ℃, separating the mixture to obtain about 5 kilograms of longevous vegetable extract, and recovering ethanol from filtrate to obtain the residue of the longevous vegetable for further extraction and reuse;
s3, crushing the hulled common anemarrhena fruit, sieving the crushed common anemarrhena fruit by a sieve of 80-100 meshes, using 50% ethanol as a solvent, wherein the solid-liquid ratio is 1: 10, fully stirring, standing for 12 hours, extracting for 30 minutes at the temperature of less than or equal to 45 ℃, separating to obtain about 3 kg of common anemarrhena fruit extract, recovering ethanol from filtrate to obtain common anemarrhena fruit residue, and keeping the common anemarrhena fruit residue for next extraction;
s4, mixing the pecan residues and the pecan residues, using 90% ethanol as a solvent, wherein the solid-liquid ratio is 1: 15, fully stirring, extracting at the temperature of less than or equal to 45 ℃ for 50 minutes, filtering off the ethanol, extracting with n-butanol to obtain n-butanol extract, concentrating the n-butanol extract by a reduced pressure evaporation method to a proper amount of water, discarding, eluting with 30-100% ethanol solution, separating the extract obtained from the eluent by HPLC, and separating to obtain about 2 kg of alkali substances;
s5, crushing the sea buckthorn, sieving the crushed sea buckthorn with a sieve of 80-100 meshes, using 60 percent ethanol as a solvent, fully stirring the mixture at a solid-liquid ratio of 1: 15, standing the mixture for 12 hours, extracting the mixture for 30 minutes at the temperature of less than or equal to 45 ℃, and recovering the ethanol from the filtrate to obtain about 4 kg of the sea buckthorn extract;
s6, crushing the kudzuvine roots, sieving the crushed kudzuvine roots with a sieve of 100 meshes and 120 meshes, using water as a solvent, fully stirring the mixture, extracting the mixture for 90 minutes at the temperature below 45 ℃ and under the vacuum degree of 0.1mmHg to 1.0mmHg under negative pressure, removing impurities from the water extract by using macroporous resin, and concentrating the water extract under reduced pressure to obtain about 3 kg of puerarin extract;
s7, crushing fructus momordicae, sieving with a 80-100 mesh sieve, using water as a solvent with a solid-to-liquid ratio of 1: 15, fully stirring, extracting at a temperature below 45 ℃ and a vacuum degree of 0.1mmHg-1.0mmHg under negative pressure for 60 minutes, removing impurities from the water extract by using macroporous resin, and concentrating under reduced pressure to obtain about 3 kg of fructus momordicae extract;
s8, smashing konjac, sieving with a 80-100-mesh sieve, using 40% ethanol as a solvent, fully stirring, standing for 12 hours, extracting at the temperature of less than or equal to 45 ℃ for 30 minutes, and recovering ethanol from the filtrate to obtain about 6 kg of glucan substances;
s9, mixing all the above materials by about 26 kg, feeding into a vacuum reduced pressure drier with a vacuum degree of 0.1-1.0 mmHg, and drying at a temperature of less than or equal to 45 deg.C to obtain extract powder by about 10 kg;
and S10, adding 1 kg of modified corn starch as an auxiliary material, fully and uniformly tabletting by using a tabletting machine to obtain about 11 kg of tablets, and packaging to obtain the finished product.
Example 4
A preparation method of vegetable and fruit plant extract for preventing and treating type II diabetes comprises the following steps:
s1, washing 50 kg of pecan, 40 kg of konjak, 30 kg of sea buckthorn, 20 kg of kudzu root and 20 kg of momordica grosvenori, shelling 20 kg of ficus carica, and drying at low temperature for later use;
s2, smashing the longevous vegetable, sieving the smashed longevous vegetable with a sieve of 80-100 meshes, using 45% ethanol as a solvent, fully stirring the mixture evenly, standing the mixture for 12 hours, extracting the mixture for 30 minutes at the temperature of less than or equal to 45 ℃, separating the mixture to obtain about 5 kilograms of longevous vegetable extract, and recovering ethanol from filtrate to obtain the residue of the longevous vegetable for further extraction and reuse;
s3, crushing the hulled common anemarrhena fruit, sieving the crushed common anemarrhena fruit by a sieve of 80-100 meshes, using 50% ethanol as a solvent, wherein the solid-liquid ratio is 1: 10, fully stirring, standing for 12 hours, extracting for 30 minutes at the temperature of less than or equal to 45 ℃, separating to obtain about 3 kg of common anemarrhena fruit extract, recovering ethanol from filtrate to obtain common anemarrhena fruit residue, and keeping the common anemarrhena fruit residue for next extraction;
s4, mixing the pecan residues and the pecan residues, using 90% ethanol as a solvent, wherein the solid-liquid ratio is 1: 15, fully stirring, extracting at the temperature of less than or equal to 45 ℃ for 50 minutes, filtering off the ethanol, extracting with n-butanol to obtain n-butanol extract, concentrating the n-butanol extract by a reduced pressure evaporation method to a proper amount of water, discarding, eluting with 30-100% ethanol solution, separating the extract obtained from the eluent by HPLC, and separating to obtain about 2 kg of alkali substances;
s5, crushing the sea buckthorn, sieving the crushed sea buckthorn with a sieve of 80-100 meshes, using 60 percent ethanol as a solvent, fully stirring the mixture at a solid-liquid ratio of 1: 15, standing the mixture for 12 hours, extracting the mixture for 30 minutes at the temperature of less than or equal to 45 ℃, and recovering the ethanol from the filtrate to obtain about 4 kg of the sea buckthorn extract;
s6, crushing the kudzuvine roots, sieving the crushed kudzuvine roots with a sieve of 100 meshes and 120 meshes, using water as a solvent, fully stirring the mixture, extracting the mixture for 90 minutes at the temperature below 45 ℃ and under the vacuum degree of 0.1mmHg to 1.0mmHg under negative pressure, removing impurities from the water extract by using macroporous resin, and concentrating the water extract under reduced pressure to obtain about 3 kg of puerarin extract;
s7, crushing fructus momordicae, sieving with a 80-100 mesh sieve, using water as a solvent with a solid-to-liquid ratio of 1: 15, fully stirring, extracting at a temperature below 45 ℃ and a vacuum degree of 0.1mmHg-1.0mmHg under negative pressure for 60 minutes, removing impurities from the water extract by using macroporous resin, and concentrating under reduced pressure to obtain about 3 kg of fructus momordicae extract;
s8, smashing konjac, sieving with a 80-100-mesh sieve, using 40% ethanol as a solvent, fully stirring, standing for 12 hours, extracting at the temperature of less than or equal to 45 ℃ for 30 minutes, and recovering ethanol from the filtrate to obtain about 6 kg of glucan substances;
s9, mixing all the above materials by about 26 kg, feeding into a vacuum reduced pressure drier with a vacuum degree of 0.1-1.0 mmHg, and drying at a temperature of less than or equal to 45 deg.C to obtain extract powder by about 10 kg;
s10, feeding about 10 kg of extract powder into a capsule filling machine, and preparing into hard capsules or soft capsules to be packaged into finished products.
Test example 1
(1) Test materials: the extract powder prepared in example 1 of the present invention.
(2) Test subjects: II, the disease course is 1-3 years for 48 cases of diabetes patients, wherein 26 cases of men and 22 cases of women.
The patients were divided into two groups of 24 patients each, 12 men and 12 women in the control group, and the mean age (55.83 ± 2.85) years. Treatment groups 14 men and 10 women, mean age (58.94 ± 2.95) years. All patients were type 2 diabetic patients with hyperlipidemia. None of the 48 patients received insulin treatment prior to the trial. The difference between the two groups of patients in terms of height, weight, sex, age, etc. is not statistically significant (P > 0.05).
(3) The treatment method comprises the following steps: the control group was tested for blood lipid and blood glucose before treatment and the diet of the patients was managed as diabetic diet, low in fat and low in salt. In addition, the patient is reminded to exercise properly after a meal; the treatment group comprises the steps of detecting blood fat and blood sugar before treatment, managing according to diabetes diet, and performing moderate exercise, and on the basis, providing the patients with oral administration of the vegetable and fruit plant extract powder of the invention, wherein the administration is respectively 8g before fasting in the morning and evening, and the administration lasts for 30 days.
(4) Evaluation criteria for therapeutic effects: has obvious effect of reducing blood fat and blood sugar obviously. Effective, the blood fat and the blood sugar are both reduced, and the reduction range is not large. And (4) invalidation: blood fat and blood sugar have no obvious change before treatment. Total effective rate (number of significant cases + number of effective cases)/total number of cases × 100%.
(5) Observation indexes are as follows: the fasting blood sugar and the postprandial blood sugar of two groups of patients are respectively detected once on 7 th day, 15 th day and 30 th day for 3 times; the glycosylated hemoglobin and the triglyceride are respectively detected once on the 15 th day and the 30 th day for 2 times; observing the specific change of fasting blood sugar, postprandial blood sugar, glycosylated hemoglobin and triglyceride of the patients between 30 days.
(6) The statistical method comprises the following steps: the SPSS16.0 statistical software is used for analyzing the data of the treatments, the measurement data is expressed by mean plus or minus standard deviation (x plus or minus s), t test is carried out, X2 test is carried out on the counting data, and the difference is statistically significant when P is less than 0.05.
(7) As a result:
TABLE 1 comparison of fasting blood glucose levels before and after treatment in two groups of patients
TABLE 1 comparison of fasting blood glucose levels before and after treatment [ FBG (mmol/L) ]two groups of patients
As can be seen from Table 2, the fasting plasma glucose levels before treatment were not significantly different (P >0.05) in the two groups of patients and were comparable. 30 days after treatment, the fasting blood sugar of the treatment group is obviously lower than that of the control group, the tangle-solidup P is less than 0.05, and the statistical significance is achieved.
TABLE 2 postprandial blood glucose level comparison before and after treatment of two groups of patients
TABLE 2 comparison of postprandial blood glucose levels before and after treatment in two groups [2hFBG (mmol/L) ]
As can be seen from Table 2, the mean 2 hour post-prandial plasma glucose levels were not significantly different (P >0.05) in both groups of patients and were comparable. After 30 days of treatment, the blood sugar of the treatment group after 2 hours after meal is obviously lower than that of the control group, the P is less than 0.05, and the statistical significance is achieved.
TABLE 3 comparison of glycated hemoglobin levels before and after treatment in two groups of patients
TABLE 3 comparison of glycated hemoglobin levels before and after treatment [ HbA1cFBG (mmol/L) ]in two groups of patients
As can be seen from Table 3, the pre-treatment glycated hemoglobin levels of the two groups of patients were comparable without significant differences (. P > 0.05). 30 days after treatment, the glycosylated hemoglobin is reduced after 30 days after the treatment group than before the treatment, the Delta P is less than 0.05, the statistical significance is achieved, the glycosylated hemoglobin is lower than that of the control group after 30 days after the treatment group, and the tangle-solidup P is less than 0.05, and the statistical significance is achieved.
TABLE 4 comparison of triglyceride levels before and after treatment in two groups of patients
TABLE 4 comparison of triglyceride levels before and after treatment [ TG (mmol/L) ]in two groups of patients
As can be seen from Table 4, there was no significant difference in the pre-treatment triglyceride levels (. SP >0.05) between the two groups of patients, which was comparable. After 30 days of treatment, the triglyceride of the treatment group is obviously lower than that of the control group, the P is less than 0.05, and the statistical significance is achieved.
TABLE 5 comparison of the results of the indices before and after treatment for two groups of patients
TABLE 5 comparison of the effects of the indices ↓ (%) before and after treatment in two groups of patients
As can be seen from Table 5, for both groups of patients, the diet of the patients was managed on a diabetic diet, and after low fat, low salt and moderate exercise, the blood glucose and triglyceride levels of the control group decreased after 30 days; under the premise of diet and exercise management, after the vegetable and fruit plant extract powder is orally taken for 30 days, all indexes are obviously reduced, and patients in a treatment group obtain better treatment effect.
In conclusion, on the basis of scientific diet and proper amount of exercise, the vegetable and fruit plant extract composition formula of the invention is orally taken by diabetics, can effectively reduce blood sugar and blood fat, and the raw materials of the invention are all from vegetable and fruit foods, can be taken for a long time, and has positive significance for preventing and treating type II diabetes.
In summary, the following steps: compared with the prior art, the invention provides the formula of the vegetable and fruit extract for preventing and treating type II diabetes and the preparation method thereof, and the formula of the vegetable and fruit extract for preventing and treating type II diabetes is applied to the field of chronic blood sugar metabolic diseases, is formed by combining pure natural vegetable and fruit plant extracts, has no toxic or side effect, is not added with external antisepsis and pigment chemical reagents, and is used for preventing and treating type II diabetes.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (1)
1. A preparation method of a vegetable and fruit composition for preventing and treating type II diabetes, which comprises 5-30 parts by weight of pecan, 4-20 parts by weight of konjak, 3-15 parts by weight of sea buckthorn, 2-10 parts by weight of garden balsam, 2-10 parts by weight of kudzu root and 2-10 parts by weight of momordica grosvenori, is characterized by comprising the following steps:
s1, washing the pecan, the konjak, the sea-buckthorn, the kudzu-vine root, the momordica grosvenori and the dehulled common macrocarpium fruit, and drying at low temperature for later use;
s2, smashing the longevous vegetable, sieving the smashed longevous vegetable with a sieve of 80-100 meshes, using 45% ethanol as a solvent, enabling the solid-liquid ratio to be 1: 10, fully and uniformly stirring, standing for 12 hours, extracting for 30 minutes at the temperature of less than or equal to 45 ℃, separating to obtain a longevous vegetable extract, recovering ethanol from filtrate to obtain a residue of the longevous vegetable, and reserving the residue for next extraction;
s3, crushing the hulled common clubmoss herb, sieving the crushed mixture by a sieve of 80-100 meshes, using 50% ethanol as a solvent, wherein the solid-liquid ratio is 1: 10, fully stirring the mixture, standing the mixture for 12 hours, extracting the mixture for 30 minutes at the temperature of less than or equal to 45 ℃, separating the mixture to obtain a common clubmoss herb extract, recovering ethanol from filtrate to obtain a common clubmoss herb residue, and keeping the common clubmoss herb residue for next extraction;
s4, mixing the pecan residues and the pecan residues, using 90% ethanol as a solvent, wherein the solid-liquid ratio is 1: 15, fully stirring, extracting at the temperature of less than or equal to 45 ℃ for 50 minutes, filtering off the ethanol, extracting with n-butanol to obtain n-butanol extract, concentrating the n-butanol extract by a reduced pressure evaporation method to a proper amount of water, washing and discarding, eluting with 30-100% ethanol solution, separating the extract obtained from the eluent by HPLC, and separating to obtain an alkaline substance;
s5, crushing the sea buckthorn, sieving the ground sea buckthorn with a sieve of 80-100 meshes, using 60 percent ethanol as a solvent, fully stirring the mixture with the solid-liquid ratio of 1: 15, standing the mixture for 12 hours, extracting the mixture for 30 minutes at the temperature of less than or equal to 45 ℃, and recovering the ethanol from the filtrate to obtain a sea buckthorn extract;
s6, crushing the kudzuvine roots, sieving the crushed kudzuvine roots with a sieve of 100 meshes and 120 meshes, using water as a solvent, fully stirring the mixture, extracting the mixture for 90 minutes at the temperature of below 45 ℃ and under the vacuum degree of 0.1mmHg to 1.0mmHg under negative pressure, removing impurities from the water extract by using macroporous resin, and concentrating the water extract under reduced pressure to obtain a puerarin extract;
s7, crushing fructus Siraitiae Grosvenorii, sieving with 80-100 mesh sieve, using water as solvent at solid-to-liquid ratio of 1: 15, stirring, extracting at 45 deg.C below and 0.1-1.0 mmHg under negative pressure for 60 min, removing impurities from the water extractive solution with macroporous resin, and concentrating under reduced pressure to obtain fructus Siraitiae Grosvenorii extract;
s8, smashing konjak, sieving with a 80-100-mesh sieve, using 40% ethanol as a solvent, fully stirring, standing for 12 hours, extracting at the temperature of less than or equal to 45 ℃ for 30 minutes, and recovering ethanol from the filtrate to obtain a glucan substance;
s9, mixing all the above materials at a certain ratio, feeding into a vacuum drying machine under reduced pressure with a vacuum degree of 0.1-1.0 mmHg, and drying at a temperature of 45 deg.C or lower to obtain extract powder.
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1504467A (en) * | 2002-12-05 | 2004-06-16 | 河北神兴沙棘研究院 | Extraction technology for seabuckthorn leaf general flavone |
| CN102204961A (en) * | 2011-04-01 | 2011-10-05 | 四川华肤生物科技有限责任公司 | Digua fig stem extract and new application thereof |
| CN102308987A (en) * | 2011-06-27 | 2012-01-11 | 新疆科宇科技有限公司 | Production method for extracting glucoside type seabuckthorn flavones from sea-buckthorn leaf |
| CN104069151A (en) * | 2014-06-03 | 2014-10-01 | 桂林三宝药业有限公司 | Preparation method of extract with high mogroside IV content |
| CN104844682A (en) * | 2015-03-16 | 2015-08-19 | 李玉山 | Method for synergistically extracting and separating effective components of fructus swietenia macrophylla in cleaning manner |
| CN105348227A (en) * | 2015-12-07 | 2016-02-24 | 西宁意格知识产权咨询服务有限公司 | Novel isocoumarins compound as well as preparation method and medical application thereof |
| CN106176888A (en) * | 2016-09-27 | 2016-12-07 | 桂林益天成生物科技有限公司 | Without returning the extracting method of bitterness Momordica-Glycosides |
| CN106432394A (en) * | 2016-09-27 | 2017-02-22 | 桂林益天成生物科技有限公司 | Method for extracting siraitia grosvenorii total saponin in siraitia grosvenorii |
| CN106699834A (en) * | 2016-12-23 | 2017-05-24 | 田野创新股份有限公司 | Method for extracting momordica glycosides from momordica grosvenori |
| CN107595903A (en) * | 2017-10-20 | 2018-01-19 | 程志鹏 | A kind of application for the method and anti-inflammation components that anti-inflammation components are extracted from purslane |
-
2018
- 2018-06-22 CN CN201810654866.2A patent/CN108553559B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1504467A (en) * | 2002-12-05 | 2004-06-16 | 河北神兴沙棘研究院 | Extraction technology for seabuckthorn leaf general flavone |
| CN102204961A (en) * | 2011-04-01 | 2011-10-05 | 四川华肤生物科技有限责任公司 | Digua fig stem extract and new application thereof |
| CN102308987A (en) * | 2011-06-27 | 2012-01-11 | 新疆科宇科技有限公司 | Production method for extracting glucoside type seabuckthorn flavones from sea-buckthorn leaf |
| CN104069151A (en) * | 2014-06-03 | 2014-10-01 | 桂林三宝药业有限公司 | Preparation method of extract with high mogroside IV content |
| CN104844682A (en) * | 2015-03-16 | 2015-08-19 | 李玉山 | Method for synergistically extracting and separating effective components of fructus swietenia macrophylla in cleaning manner |
| CN105348227A (en) * | 2015-12-07 | 2016-02-24 | 西宁意格知识产权咨询服务有限公司 | Novel isocoumarins compound as well as preparation method and medical application thereof |
| CN106176888A (en) * | 2016-09-27 | 2016-12-07 | 桂林益天成生物科技有限公司 | Without returning the extracting method of bitterness Momordica-Glycosides |
| CN106432394A (en) * | 2016-09-27 | 2017-02-22 | 桂林益天成生物科技有限公司 | Method for extracting siraitia grosvenorii total saponin in siraitia grosvenorii |
| CN106699834A (en) * | 2016-12-23 | 2017-05-24 | 田野创新股份有限公司 | Method for extracting momordica glycosides from momordica grosvenori |
| CN107595903A (en) * | 2017-10-20 | 2018-01-19 | 程志鹏 | A kind of application for the method and anti-inflammation components that anti-inflammation components are extracted from purslane |
Non-Patent Citations (9)
| Title |
|---|
| 中国的一款新型降糖食品问世，对2型糖尿病"效果空前";3N507;《https://tieba.baidu.com/p/5689504613》;20180508;第1-3页，尤其是3N507发的帖子 * |
| 乙醇提纯魔芋葡甘聚糖的研究;彭湘莲等;《食品科技》;20101231;第35卷(第5期);第248-251页 * |
| 向天果化学成分研究;刘劲松等;《中药材》;20160731;第39卷(第7期);第1530-1535页 * |
| 盐碱地马齿苋不同提取液的抗氧化活性研究;王仁雷等;《食品工业科技》;20131231;第34卷(第17期);第49-51、55页 * |
| 罗汉果的果实与其茎叶提取物质量分析及药效学比较研究;饶荣;《中国优秀硕士学位论文全文数据库 医药卫生科技辑》;20130215(第2期);第E057-103页，尤其是摘要第1页最后1段 * |
| 马齿苋不同提取物对D-半乳糖致衰老小鼠空间学习能力的影响;金英子等;《中国实验方剂学杂志》;20110630;第17卷(第12期);第178-181页 * |
| 马齿苋抗糖尿病活性成分的研究;金妍等;《中成药》;20150131;第37卷(第1期);第124-128页 * |
| 马齿苋的化学成分;丁怀伟等;《沈阳药科大学学报》;20091130;第26卷(第11期);第878-881页 * |
| 魔芋葡甘聚糖超声波辅助纯化工艺研究;陈建福等;《佳木斯大学学报(自然科学版)》;20131130;第31卷(第6期);第964-967页 * |
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