CN106176888A - Without returning the extracting method of bitterness Momordica-Glycosides - Google Patents
Without returning the extracting method of bitterness Momordica-Glycosides Download PDFInfo
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- CN106176888A CN106176888A CN201610860141.XA CN201610860141A CN106176888A CN 106176888 A CN106176888 A CN 106176888A CN 201610860141 A CN201610860141 A CN 201610860141A CN 106176888 A CN106176888 A CN 106176888A
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- glycosides
- momordica
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention discloses a kind of nothing and return the extracting method of bitterness Momordica-Glycosides, comprise the following steps: 1) Momordica-Glycosides is dissolved in water after, upper DM130 macroporous resin, first wash with water to colourless, then with ethanol elution, collect ethanol elution, eluent is reclaimed ethanol, obtains extractum；2) extractum is dissolved in water, upper D101+ macroporous resin, wash with water, collect eluent, concentrate, be dried, to obtain final product.Compared with prior art, without bitter substance in the Momordica-Glycosides that the present invention extracts, sweet taste is pure, without returning bitterness.
Description
Technical field
The invention belongs to technical field of biological extraction, be specifically related to a kind of nothing and return the extracting method of bitterness Momordica-Glycosides.
Background technology
Fructus Momordicae Siraitia grosvenorii, belongs to the fruit of Dicotyledoneae Cucurbitaceae Genus Siraitia Merr, main
The ground such as Guangxi to be originated in, Guangdong, Hunan, Jiangxi.The dry fruit of Fructus Momordicae is oval or circular, shell sepia or sootiness
Color, with a large amount of tiny black pubescences, more bigger than goose egg.Dry fruit is relatively light, and seed, peel and sarcocarp all can be used as medicine.Fructus Momordicae
High nutritive value, containing substantial amounts of saccharide, protein, vitamin, fatty acid, the aminoacid of multiple needed by human and trace unit
Element.
Lo Han Guo fruit separates and has identified siamenoside I (sianenoside I), Momordica-Glycosides II E
(eogroside II E), Momordica-Glycosides III (mogroside III), Momordica-Glycosides III E (mogroside III E), Fructus Momordicae are sweet
The cucurbitane elements three such as glycoside IV (ogroside IV), mogroside Ⅴ (mogroside V), Momordica-Glycosides A, Fructus Momordicae Neogroside
Terpene glycoside material, except Momordica-Glycosides IV is without sweet taste, Fructus Momordicae Neogroside is outside bitterness, is sweet substance, and wherein Fructus Momordicae is sweet
The sugariness of glycoside V is the highest, is equivalent to 300 times of sucrose, and sweet taste is pure, free from extraneous odour, aftertaste sense are strong, Heat stability is good, can conduct
Food, the sweeting agent of dish, be the preferable sugar Dai Pin of diabetes patient.Mogroside Ⅴ Heat stability is good, at 100 DEG C of aqueous solutions
In stable, still will not be destroyed at 120 DEG C of more than laser heating 12h, belong to non-fermented material, be beneficial to process storage.
At present, obtaining the method for sweet glycoside from Fructus Momordicae mainly has a following two, and one is that water carries and adds resin partition method, by sieve
Load after Chinese fruit is broken in extraction pot, extracting in water, after repeatedly extracting, united extraction liquid, filter, upper resin carries out absorption point
From, by ethanol or methanol-eluted fractions, after eluent removes alcohol, spray drying forms；Two is alcohol extraction membrane separation process, and its technique is by arhat
Fruit is broken, loads in extraction pot, adds ethanol and extracts, after repeatedly extracting, united extraction liquid, filters, filtrate is passed through film
Separating and concentrate, concentrated solution vacuum drying forms.But containing Fructus Momordicae Neogroside in the Momordica-Glycosides of said method extraction, cause
Momordica-Glycosides has strong rear bitterness, have impact on Fructus Momordicae and put forward the mouthfeel of sweet glycoside and limit it as natural sweetener
Application.
Summary of the invention
Present invention solves the technical problem that with strong rear bitterness in the Momordica-Glycosides being to overcome prior art to extract,
A kind of nothing is provided to return the extracting method of bitterness Momordica-Glycosides.
The technical scheme that the present invention provides is the extracting method without returning bitterness Momordica-Glycosides, comprises the following steps:
1) after Momordica-Glycosides being dissolved in water, upper DM130 macroporous resin, first wash with water to colourless, then use ethanol
Eluting, collects ethanol elution, eluent is reclaimed ethanol, obtains extractum；
2) extractum is dissolved in water, upper D101+ macroporous resin, wash with water, collect eluent, concentrate, be dried, to obtain final product.
The preparation method of described Momordica-Glycosides is as follows: Fructus Momordicae adds its weight 5～the alcohol reflux 3 of 10 times
Secondary, extraction 1～2h every time, united extraction liquid, filters, collects filtrate, reclaim ethanol, be dried, be Momordica-Glycosides.Fructus Momordicae
In containing substantial amounts of protein, glucose and fructose, employing ethanol is solvent, and ethanol is unfavorable for the dissolving of protein and fructose,
The dissolution of impurity can be reduced, useful to subsequent purification.Ethanol volumetric concentration is 40～50% to be preferred, and the too high meeting of concentration of alcohol is right
Protein produces cohesion so that protein is trapped in the internal gutter of granule, increases the diffusion of Momordica-Glycosides on the contrary
Resistance, reduces the yield of finished product.The too low then impurity content of concentration of alcohol is relatively big, affects the purity of finished product.
Step 1) in, the consumption of water is 8～15 times of Momordica-Glycosides weight.DM130 macroporous adsorbent resin on first time
Post, the Momordica-Glycosides in adsorbable solution and a small amount of pigment composition, on solution, column flow rate is 0.3～0.5BV/h, water
Elution flow rate is 1～2BV/h, washes with water and can preferentially be eluted by pigment, and the elution speed of ethanol is 0.5～0.6BV/h,
The Momordica-Glycosides adsorbed in resin can be eluted.Ethanol volumetric concentration is 75～80% to be advisable.
Step 2) in, according to molecular mass, size and the polarity of Fructus Momordicae Neogroside, D101 macroporous resin is changed
Enter so that it is the absorption affinity of Fructus Momordicae Neogroside is strengthened, and the absorption affinity of other sweet glycosides is weakened, when using water to carry out eluting,
Other saponin in addition to Fructus Momordicae Neogroside are more weak with the absorption affinity of macroporous resin, are preferentially eluted out, and Fructus Momordicae Neogroside is then
Stay in resin, thus reach to slough the effect of Fructus Momordicae Neogroside.
Described D101+ resin is the nonpolar copolymer of styrene type, and its technical parameter is:
1) outward appearance: the opaque spherical particle of milky；
2) particle size range 0.3～1.25mm >=95%；
3) water content: 60～75%；
4) wet true density: 1.05～1.15g/ml；
5) wet apparent density: 0.60～0.65g/ml；
6) specific surface area: 520～550/g；
7) average pore size:
8) porosity: 42～46%；
9) pore volume: 1.18～1.24ml/g.
Step 2) in, the consumption of water is 10～15 times of extractum weight.On solution, column flow rate is 0.3～0.5BV/h, water
Elution flow rate is 0.5～0.6BV/h.
Compared with prior art, not containing Fructus Momordicae Neogroside in the Momordica-Glycosides that the present invention extracts, sweet taste is pure, without returning
Bitterness.
Detailed description of the invention
The present invention is further elaborated for specific examples below, but not as a limitation of the invention.
Embodiment 1
1) Fructus Momordicae is pulverized, add the alcohol reflux that volumetric concentration is 40% 3 times of its weight 5 times, carry every time
Take 1h, united extraction liquid, filter, take filtrate recycling ethanol, be dried, be Momordica-Glycosides；
2) upper DM130 macroporous resin after adding the water dissolution of its weight 8 times in Momordica-Glycosides, on solution, column flow rate is
0.3BV/h, first washes with water to colourless, and the elution flow rate of water is 1BV/h, is then the ethanol elution of 75% by volumetric concentration,
The elution speed of ethanol is 0.5BV/h, collects ethanol elution, eluent is reclaimed ethanol, obtains extractum；
3) extractum adds the water dissolution of its weight 10 times, upper D101+ macroporous resin, and on solution, column flow rate is 0.3BV/h,
Washing with water, the elution flow rate of water is 0.5BV/h, collects water elution liquid, concentrates, and is dried, obtains Momordica-Glycosides.Through tasting,
Momordica-Glycosides sweet taste is pure, without returning bitterness.Momordica-Glycosides carries out HPLC analysis, and wherein the content of Fructus Momordicae Neogroside is
0.001%.
Embodiment 2
1) Fructus Momordicae is pulverized, add the alcohol reflux that volumetric concentration is 50% 3 times of its weight 10 times, carry every time
Take 2h, united extraction liquid, filter, take filtrate recycling ethanol, be dried, be Momordica-Glycosides；
2) upper DM130 macroporous resin, column flow rate on solution after adding the water dissolution of its weight 15 times in Momordica-Glycosides
For 0.5BV/h, first washing with water to colourless, the elution flow rate of water is 2BV/h, then washes with the ethanol that volumetric concentration is 80%
De-, the elution speed of ethanol is 0.6BV/h, collects ethanol elution, eluent is reclaimed ethanol, obtains extractum；
3) extractum adds the water dissolution of its weight 15 times, upper D101+ macroporous resin, and on solution, column flow rate is 0.5BV/h,
Washing with water, the elution flow rate of water is 0.6BV/h, collects water elution liquid, concentrates, and is dried, obtains Momordica-Glycosides.Through tasting,
Momordica-Glycosides sweet taste is pure, without returning bitterness.Momordica-Glycosides carries out HPLC analysis, and wherein the content of Fructus Momordicae Neogroside is
0.002%.
Embodiment 3
1) Fructus Momordicae is pulverized, add the alcohol reflux that volumetric concentration is 45% 3 times of its weight 8 times, carry every time
Take 1.5h, united extraction liquid, filter, take filtrate recycling ethanol, be dried, be Momordica-Glycosides；
2) upper DM130 macroporous resin, column flow rate on solution after adding the water dissolution of its weight 10 times in Momordica-Glycosides
For 0.4BV/h, first washing with water to colourless, the elution flow rate of water is 1.5BV/h, then washes with the ethanol that volumetric concentration is 78%
De-, the elution speed of ethanol is 0.55BV/h, collects ethanol elution, eluent is reclaimed ethanol, obtains extractum；
3) extractum adds the water dissolution of its weight 12 times, upper D101+ macroporous resin, and on solution, column flow rate is 0.4BV/h,
Washing with water, the elution flow rate of water is 0.55BV/h, collects water elution liquid, concentrates, and is dried, obtains Momordica-Glycosides.Through tasting,
Momordica-Glycosides sweet taste is pure, without returning bitterness.Momordica-Glycosides carries out HPLC analysis, and wherein the content of Fructus Momordicae Neogroside is
0.0018%.
Embodiment 4
1) Fructus Momordicae is pulverized, add the alcohol reflux that volumetric concentration is 50% 3 times of its weight 5 times, carry every time
Take 1h, united extraction liquid, filter, take filtrate recycling ethanol, be dried, be Momordica-Glycosides；
2) upper DM130 macroporous resin, column flow rate on solution after adding the water dissolution of its weight 15 times in Momordica-Glycosides
For 0.3BV/h, first washing with water to colourless, the elution flow rate of water is 2BV/h, then washes with the ethanol that volumetric concentration is 75%
De-, the elution speed of ethanol is 0.6BV/h, collects ethanol elution, eluent is reclaimed ethanol, obtains extractum；
3) extractum adds the water dissolution of its weight 10 times, upper D101+ macroporous resin, and on solution, column flow rate is 0.5BV/h,
Washing with water, the elution flow rate of water is 0.5BV/h, collects water elution liquid, concentrates, and is dried, obtains Momordica-Glycosides.Through tasting,
Momordica-Glycosides sweet taste is pure, without returning bitterness.Momordica-Glycosides carries out HPLC analysis, and wherein the content of Fructus Momordicae Neogroside is
0.0014%.
Claims (9)
1. without returning the extracting method of bitterness Momordica-Glycosides, it is characterised in that: comprise the following steps:
1) after Momordica-Glycosides being dissolved in water, upper DM130 macroporous resin, first wash with water to colourless, then with ethanol elution,
Collect ethanol elution, eluent is reclaimed ethanol, obtains extractum；
2) extractum is dissolved in water, upper D101+ macroporous resin, wash with water, collect eluent, concentrate, be dried, to obtain final product.
Nothing the most according to claim 1 returns the extracting method of bitterness Momordica-Glycosides, it is characterised in that: step 1) in, sieve
The preparation method of the Chinese sweet glycoside of fruit is: Fructus Momordicae adds the alcohol reflux 3 times of its weight 5～10 times, extract every time 1～
2h, united extraction liquid, filters, by filtrate recycling ethanol, is dried, to obtain final product.
Nothing the most according to claim 2 returns the extracting method of bitterness Momordica-Glycosides, it is characterised in that: ethanol volumetric concentration
It is 40～50%.
Nothing the most according to claim 1 returns the extracting method of bitterness Momordica-Glycosides, it is characterised in that: step 1) in, second
The volumetric concentration of alcohol is 75～80%.
Nothing the most according to claim 1 returns the extracting method of bitterness Momordica-Glycosides, it is characterised in that: step 1) in, water
Consumption is Momordica-Glycosides weight 8～15 times.
Nothing the most according to claim 1 returns the extracting method of bitterness Momordica-Glycosides, it is characterised in that: step 1) in, molten
On liquid, column flow rate is 0.3～0.5BV/h, and the elution flow rate of water is 1～2BV/h, and the elution speed of ethanol is 0.5～0.6BV/h.
Nothing the most according to claim 1 returns the extracting method of bitterness Momordica-Glycosides, it is characterised in that: step 2) in, institute
Stating D101+ resin is the nonpolar copolymer of styrene type, and its technical parameter is:
1) outward appearance: the opaque spherical particle of milky；
2) particle size range 0.3～1.25mm >=95%；
3) water content: 60～75%；
4) wet true density: 1.05～1.15g/ml；
5) wet apparent density: 0.60～0.65g/ml；
6) specific surface area: 520～550/g；
7) average pore size:
8) porosity: 42～46%；
9) pore volume: 1.18～1.24ml/g.
Nothing the most according to claim 1 returns the extracting method of bitterness Momordica-Glycosides, it is characterised in that: step 2) in, water
Consumption is extractum weight 10～15 times.
Nothing the most according to claim 1 returns the extracting method of bitterness Momordica-Glycosides, it is characterised in that: step 2) in, molten
On liquid, column flow rate is 0.3～0.5BV/h, and the elution flow rate of water is 0.5～0.6BV/h.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610860141.XA CN106176888A (en) | 2016-09-27 | 2016-09-27 | Without returning the extracting method of bitterness Momordica-Glycosides |
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| CN201610860141.XA CN106176888A (en) | 2016-09-27 | 2016-09-27 | Without returning the extracting method of bitterness Momordica-Glycosides |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108553559A (en) * | 2018-06-22 | 2018-09-21 | 南昌糖友安健康科技有限公司 | A kind of vegetables and fruits extraction composition formula and preparation method for preventing type II diabetes |
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| CN101007042A (en) * | 2007-02-06 | 2007-08-01 | 桂林莱茵生物科技股份有限公司 | A preparation method of debittered and discolored siraitia grosvenorii extracts |
| WO2013040953A1 (en) * | 2011-09-19 | 2013-03-28 | 桂林莱茵生物科技股份有限公司 | Sweetener comprising total momordica grosvenori glycoside and preparation method thereof |
| CN104069152A (en) * | 2014-06-05 | 2014-10-01 | 桂林三宝生物科技有限公司 | Preparation method of extract with high mogroside IV content |
| CN104069151A (en) * | 2014-06-03 | 2014-10-01 | 桂林三宝药业有限公司 | Preparation method of extract with high mogroside IV content |
-
2016
- 2016-09-27 CN CN201610860141.XA patent/CN106176888A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101007042A (en) * | 2007-02-06 | 2007-08-01 | 桂林莱茵生物科技股份有限公司 | A preparation method of debittered and discolored siraitia grosvenorii extracts |
| WO2013040953A1 (en) * | 2011-09-19 | 2013-03-28 | 桂林莱茵生物科技股份有限公司 | Sweetener comprising total momordica grosvenori glycoside and preparation method thereof |
| CN104069151A (en) * | 2014-06-03 | 2014-10-01 | 桂林三宝药业有限公司 | Preparation method of extract with high mogroside IV content |
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Non-Patent Citations (1)
| Title |
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| 农毅清等: "罗汉果甜苷提取工艺的研究", 《时珍国医国药》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108553559A (en) * | 2018-06-22 | 2018-09-21 | 南昌糖友安健康科技有限公司 | A kind of vegetables and fruits extraction composition formula and preparation method for preventing type II diabetes |
| CN108553559B (en) * | 2018-06-22 | 2021-03-23 | 南昌糖友安健康科技有限公司 | Formula and preparation method of vegetable and fruit extract for preventing and treating type II diabetes |
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Application publication date: 20161207 |