CN106432394A - Method for extracting siraitia grosvenorii total saponin in siraitia grosvenorii - Google Patents
Method for extracting siraitia grosvenorii total saponin in siraitia grosvenorii Download PDFInfo
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- CN106432394A CN106432394A CN201610860138.8A CN201610860138A CN106432394A CN 106432394 A CN106432394 A CN 106432394A CN 201610860138 A CN201610860138 A CN 201610860138A CN 106432394 A CN106432394 A CN 106432394A
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- extract
- water
- momordica grosvenori
- momorside
- ethanol
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- 241001409321 Siraitia grosvenorii Species 0.000 title claims abstract description 47
- 235000011171 Thladiantha grosvenorii Nutrition 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000001397 quillaja saponaria molina bark Substances 0.000 title abstract description 5
- 229930182490 saponin Natural products 0.000 title abstract description 5
- 150000007949 saponins Chemical class 0.000 title abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 101
- 239000000284 extract Substances 0.000 claims abstract description 55
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 53
- 238000010828 elution Methods 0.000 claims abstract description 29
- 239000011347 resin Substances 0.000 claims abstract description 22
- 229920005989 resin Polymers 0.000 claims abstract description 22
- 239000003480 eluent Substances 0.000 claims description 9
- 239000000706 filtrate Substances 0.000 claims description 9
- 239000012141 concentrate Substances 0.000 claims description 8
- 238000004064 recycling Methods 0.000 claims description 7
- 239000012467 final product Substances 0.000 claims description 6
- 238000010992 reflux Methods 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims description 5
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 3
- 239000012798 spherical particle Substances 0.000 claims description 2
- 235000019441 ethanol Nutrition 0.000 abstract description 47
- 238000000605 extraction Methods 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract 2
- 239000000243 solution Substances 0.000 description 11
- 235000019658 bitter taste Nutrition 0.000 description 8
- 235000019605 sweet taste sensations Nutrition 0.000 description 7
- 238000010521 absorption reaction Methods 0.000 description 6
- 235000013399 edible fruits Nutrition 0.000 description 6
- 235000019640 taste Nutrition 0.000 description 6
- 239000000047 product Substances 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 235000009508 confectionery Nutrition 0.000 description 4
- 229930182478 glucoside Natural products 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- GHBNZZJYBXQAHG-KUVSNLSMSA-N (2r,3r,4s,5s,6r)-2-[[(2r,3s,4s,5r,6r)-6-[[(3s,8s,9r,10r,11r,13r,14s,17r)-17-[(2r,5r)-5-[(2s,3r,4s,5s,6r)-4,5-dihydroxy-3-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-[[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@H](CC[C@@H](C)[C@@H]1[C@]2(C[C@@H](O)[C@@]3(C)[C@H]4C(C([C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]6[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O6)O)O5)O)CC4)(C)C)=CC[C@H]3[C@]2(C)CC1)C)C(C)(C)O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O GHBNZZJYBXQAHG-KUVSNLSMSA-N 0.000 description 3
- 150000008131 glucosides Chemical class 0.000 description 3
- TVJXHJAWHUMLLG-UHFFFAOYSA-N mogroside V Natural products CC(CCC(OC1OC(COC2OC(CO)C(O)C(O)C2OC3OC(CO)C(O)C(O)C3O)C(O)C(O)C1O)C(C)(C)O)C4CCC5(C)C6CC=C7C(CCC(OC8OC(COC9OC(CO)C(O)C(O)C9O)C(O)C(O)C8O)C7(C)C)C6(C)C(O)CC45C TVJXHJAWHUMLLG-UHFFFAOYSA-N 0.000 description 3
- -1 Terpene glucoside Chemical class 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- XJIPREFALCDWRQ-UYQGGQRHSA-N (2r,3r,4s,5s,6r)-2-[[(2r,3s,4s,5r,6s)-3,4-dihydroxy-6-[(3r,6r)-2-hydroxy-6-[(3s,8s,9r,10r,11r,13r,14s,17r)-11-hydroxy-4,4,9,13,14-pentamethyl-3-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-2,3,7,8,10,11,12,15,16,17-decahydro-1h-cyclop Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@H](CC[C@@H](C)[C@@H]1[C@]2(C[C@@H](O)[C@@]3(C)[C@H]4C(C([C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)CC4)(C)C)=CC[C@H]3[C@]2(C)CC1)C)C(C)(C)O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O XJIPREFALCDWRQ-UYQGGQRHSA-N 0.000 description 1
- KYVIPFHNYCKOMQ-YMRJDYICSA-N (2r,3s,4s,5r,6r)-2-(hydroxymethyl)-6-[[(2r,3s,4s,5r,6s)-3,4,5-trihydroxy-6-[(3r,6r)-2-hydroxy-6-[(3s,8s,9r,10r,11r,13r,14s,17r)-11-hydroxy-4,4,9,13,14-pentamethyl-3-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-2,3,7,8,10,11,12,15,16,1 Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)O[C@H](CC[C@@H](C)[C@@H]1[C@]2(C[C@@H](O)[C@@]3(C)[C@H]4C(C([C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)CC4)(C)C)=CC[C@H]3[C@]2(C)CC1)C)C(C)(C)O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O KYVIPFHNYCKOMQ-YMRJDYICSA-N 0.000 description 1
- QATISCJMIITVAB-UHFFFAOYSA-N 2-[[17-[5-[4,5-dihydroxy-6-(hydroxymethyl)-3-[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-6-hydroxy-6-methylheptan-2-yl]-11-hydroxy-4,4,9,13,14-pentamethyl-2,3,7,8,10,11,12,15,16,17-decahydro-1h-cyclopenta[a]phenanthren-3-yl]oxy]-6-(hydro Chemical compound C1CC2(C)C3CC=C(C(C(OC4C(C(O)C(O)C(CO)O4)O)CC4)(C)C)C4C3(C)C(O)CC2(C)C1C(C)CCC(C(C)(C)O)OC1OC(CO)C(O)C(O)C1OC1OC(CO)C(O)C(O)C1O QATISCJMIITVAB-UHFFFAOYSA-N 0.000 description 1
- 241000272814 Anser sp. Species 0.000 description 1
- 206010013911 Dysgeusia Diseases 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- KYVIPFHNYCKOMQ-UHFFFAOYSA-N Mogroside III Natural products C1CC2(C)C3CC=C(C(C(OC4C(C(O)C(O)C(CO)O4)O)CC4)(C)C)C4C3(C)C(O)CC2(C)C1C(C)CCC(C(C)(C)O)OC(C(C(O)C1O)O)OC1COC1OC(CO)C(O)C(O)C1O KYVIPFHNYCKOMQ-UHFFFAOYSA-N 0.000 description 1
- 241000238370 Sepia Species 0.000 description 1
- 241001409305 Siraitia Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- ZYZJWAJOTPNVPI-ZVBSCDOUSA-N cucurbitane Chemical compound C([C@H]1[C@]2(C)CC[C@@H]([C@]2(CC[C@]11C)C)[C@H](C)CCCC(C)C)CC2[C@H]1CCCC2(C)C ZYZJWAJOTPNVPI-ZVBSCDOUSA-N 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000004093 laser heating Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 235000021096 natural sweeteners Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- XJIPREFALCDWRQ-UHFFFAOYSA-N siamenoside I Natural products C1CC2(C)C3CC=C(C(C(OC4C(C(O)C(O)C(CO)O4)O)CC4)(C)C)C4C3(C)C(O)CC2(C)C1C(C)CCC(C(C)(C)O)OC(C(C(O)C1O)OC2C(C(O)C(O)C(CO)O2)O)OC1COC1OC(CO)C(O)C(O)C1O XJIPREFALCDWRQ-UHFFFAOYSA-N 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J17/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, having an oxygen-containing hetero ring not condensed with the cyclopenta(a)hydrophenanthrene skeleton
- C07J17/005—Glycosides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a method for extracting siraitia grosvenorii total saponin in siraitia grosvenorii. The method includes the following steps that firstly, siraitia grosvenorii is added into ethyl alcohol to be extracted, an extraction solution is filtered, ethyl alcohol in the extraction solution is recycled, and an extract A is obtained; secondly, the extract A is added into water to be dissolved, then D201 macroporous resin is added, the mixture is eluted with water to be colorless and then eluted with ethyl alcohol, an ethyl alcohol elution solution is collected, ethyl alcohol is recycled from the elution solution, and an extract B is obtained; thirdly, the extract B is added into water to be dissolved, D101+ macroporous resin is added, the mixture is eluted with water, an elution solution is collected, concentrated and dried, and siraitia grosvenorii total saponin is obtained. Compared with the prior art, the siraitia grosvenorii total saponin extracted through the method does not contain bitter substances and is pure in sweetness and free of rebitter.
Description
Technical field
The invention belongs to technical field of biological extraction and in particular to a kind of from Momordica grosvenori extract momorside side
Method.
Background technology
Momordica grosvenori Siraitia grosvenorii, belongs to the fruit of Dicotyledoneae Curcurbitaceae Genus Siraitia Merr, main
The ground such as Guangxi to be originated in, Guangdong, Hunan, Jiangxi.The dry fruit of Momordica grosvenori is oval or circular, shell sepia or sootiness
Color, with tiny black pubescence in a large number, more bigger than goose egg.Dry fruit is lighter, and seed, pericarp and pulp all can be used as medicine.Momordica grosvenori
High in nutritive value, containing substantial amounts of carbohydrate, protein, vitamin, aliphatic acid, multiple amino acid needed by human and micro unit
Element.
Separate in Lo Han Guo fruit and identify siamenoside I (sianenoside I), Momordica-Glycosides II E
(eogroside II E), Momordica-Glycosides III (mogroside III), Momordica-Glycosides III E (mogroside III E), sweet Momordica grosvenori
The cucurbitane elements three such as glucoside IV (ogroside IV), mogroside Ⅴ (mogroside V), Momordica-Glycosides A, Momordica grosvenori Neogroside
Terpene glucoside material, except Momordica-Glycosides IV no sweet taste, Momordica grosvenori Neogroside is outside bitter taste, is sweet substance, wherein sweet Momordica grosvenori
The sugariness highest of glucoside V, is equivalent to 300 times of sucrose, and sweet taste is pure, free from extraneous odour, aftertaste sense are strong, heat endurance is good, can conduct
Food, the sweetener of dish, are the preferable sugar Dai Pin of diabetes patient.Mogroside Ⅴ heat endurance is good, in 100 DEG C of aqueous solution
In stable, still will not be destroyed in 120 DEG C of more than laser heating 12h, belong to non-fermented material, beneficial to processing storage.
At present, mainly have following two from the preparation method of momorside, one is that water extraction adds resin partition method, by sieve
Load after Chinese fruit is broken in extractor, extracting in water, after repeatedly extracting, merge extract, filter, upper resin carries out absorption point
From with ethanol or methanol-eluted fractions, after eluent removes alcohol, spray drying forms；Two is alcohol extracting membrane separation process, and its technique is by arhat
Fruit is broken, loads in extractor, adds ethanol to be extracted, and after repeatedly extracting, merges extract, filters, filtrate is passed through film
Separate and concentrate, concentrate vacuum drying forms.But contain Momordica grosvenori Neogroside in the prepared momorside of said method, lead
Cause there is strong rear bitter taste in extract, have impact on the mouthfeel of momorside and limit its answering as natural sweetener
With.
Content of the invention
Present invention solves the technical problem that being with strong rear hardship in the Fructus Monordicae extract overcome prior art extraction
Taste, provides a kind of good taste, the no preparation method of the momorside of bitter taste.
The technical scheme that the present invention provides is the method extracting momorside from Momordica grosvenori, comprises the following steps：
1) Momordica grosvenori is added alcohol extract, extract is filtered, take filtrate recycling ethanol, obtain extractum A；
2) upper D201 macroreticular resin after extractum A being dissolved in water, first washes with water to colourless, then uses ethanol elution, receive
Collection ethanol eluate, eluent is reclaimed ethanol, obtains medicinal extract B；
3) medicinal extract B is dissolved in water, upper D101+ macroreticular resin, wash with water, collect eluent, concentrate, be dried, obtain final product.
Step 1) in, the alcohol reflux that Momordica grosvenori is added 5～10 times of its weight extracts 3 times, extracts 1～2h every time, closes
And extract, filter, collect filtrate, reclaim ethanol, as extractum A.Substantial amounts of protein, glucose and fruit is contained in Momordica grosvenori
Sugar, is solvent using ethanol, and ethanol is unfavorable for the dissolving of protein and fructose, it is possible to reduce the dissolution of impurity, to subsequent purification
Beneficial.Ethanol volumetric concentration is preferred for 40～50%, and concentration of alcohol is too high to produce cohesion so that protein to protein
It is trapped in the internal gutter of particle, increases the diffusional resistance of Momordica-Glycosides on the contrary, reduce the yield of finished product.Concentration of alcohol
Too low then impurity content is larger, the purity of impact finished product.
Step 2) in, the consumption of water is 8～15 times of extractum A weight.D201 large pore resin absorption column on first time, can inhale
Momordica-Glycosides in attached solution and a small amount of pigment constituents, on solution, column flow rate is 0.3～0.5BV/h, the wash-out stream of water
Speed is 1～2BV/h, washes with water and preferentially can elute pigment, and the elution speed of ethanol is 0.5～0.6BV/h, can will set
In fat, the momorside of absorption elutes.Ethanol volumetric concentration is advisable for 75～80%.
Step 3) in, according to the molecular mass of Momordica grosvenori Neogroside, size and polarity, D101 macroreticular resin is changed
Enter so as to strengthen to the absorption affinity of Momordica grosvenori Neogroside, and the absorption affinity of other sweet glucosides is weakened, when being eluted using water,
Other saponin(es in addition to Momordica grosvenori Neogroside are weaker with the absorption affinity of macroreticular resin, be preferentially eluted out, and Momordica grosvenori Neogroside is then
Stay in resin, thus reaching the effect sloughing Momordica grosvenori Neogroside.
Described D101+ resin is the nonpolar EVA of styrene type, and its technical parameter is：
1) outward appearance：The opaque spherical particle of milky；
2) particle size range 0.3～1.25mm >=95%；
3) water content：60～75%；
4) wet true density：1.05～1.15g/ml；
5) wet apparent density：0.60～0.65g/ml；
6) specific surface area：520～550/g；
7) average pore size：
8) porosity：42～46%；
9) pore volume：1.18～1.24ml/g.
Step 3) in, the consumption of water is 10～15 times of medicinal extract B weight.On solution, column flow rate is 0.3～0.5BV/h, water
Elution flow rate be 0.5～0.6BV/h.
Compared with prior art, do not contain Momordica grosvenori Neogroside in the momorside that the present invention extracts, sweet taste is pure, no
Return bitter taste.
Specific embodiment
The present invention is further elaborated for specific examples below, but not as a limitation of the invention.
Embodiment 1
1) volumetric concentration that Momordica grosvenori is added 5 times of its weight be 40% alcohol reflux extract 3 times, every time extract 1h,
Merge extract, filter, take filtrate recycling ethanol, obtain extractum A；
2) extractum A is added upper D201 macroreticular resin after the water dissolves of 8 times of its weight, on solution, column flow rate is 0.3BV/h,
First wash with water to colourless, the elution flow rate of water is 1BV/h, the ethanol elution being then 75% with volumetric concentration, the washing of ethanol
De- speed is 0.5BV/h, collects ethanol eluate, eluent is reclaimed ethanol, obtains medicinal extract B；
3) medicinal extract B is added the water dissolves of 10 times of its weight, upper D101+ macroreticular resin, on solution, column flow rate is 0.3BV/
H, washes with water, and the elution flow rate of water is 0.5BV/h, collects water elution, concentrates, is dried, obtains final product momorside.Through product
Taste, momorside sweet taste is pure, no return bitter taste.Momorside carried out HPLC analysis, wherein Momordica grosvenori Neogroside
Content is 0.001%.
Embodiment 2
1) volumetric concentration that Momordica grosvenori is added 10 times of its weight be 50% alcohol reflux extract 3 times, every time extract 2h,
Merge extract, filter, take filtrate recycling ethanol, obtain extractum A；
2) extractum A is added upper D201 macroreticular resin after the water dissolves of 15 times of its weight, on solution, column flow rate is 0.5BV/
H, first washes with water to colourless, and the elution flow rate of water is 2BV/h, the ethanol elution being then 80% with volumetric concentration, ethanol
Elution speed is 0.6BV/h, collects ethanol eluate, eluent is reclaimed ethanol, obtains medicinal extract B；
3) medicinal extract B is added the water dissolves of 15 times of its weight, upper D101+ macroreticular resin, on solution, column flow rate is 0.5BV/
H, washes with water, and the elution flow rate of water is 0.6BV/h, collects water elution, concentrates, is dried, obtains final product momorside.Through product
Taste, momorside sweet taste is pure, no return bitter taste.Momorside carried out HPLC analysis, wherein Momordica grosvenori Neogroside
Content is 0.002%.
Embodiment 3
1) volumetric concentration that Momordica grosvenori is added 8 times of its weight is that 45% alcohol reflux extracts 3 times, extracts every time
1.5h, merges extract, filters, takes filtrate recycling ethanol, obtain extractum A；
2) extractum A is added upper D201 macroreticular resin after the water dissolves of 12 times of its weight, on solution, column flow rate is 0.4BV/
H, first washes with water to colourless, and the elution flow rate of water is 1.5BV/h, the ethanol elution being then 78% with volumetric concentration, ethanol
Elution speed be 0.55BV/h, collect ethanol eluate, eluent is reclaimed ethanol, obtains medicinal extract B；
3) medicinal extract B is added the water dissolves of 12 times of its weight, upper D101+ macroreticular resin, on solution, column flow rate is 0.4BV/
H, washes with water, and the elution flow rate of water is 0.55BV/h, collects water elution, concentrates, is dried, obtains final product momorside.Warp
Taste, momorside sweet taste is pure, no return bitter taste.Momorside is carried out HPLC analysis, wherein Momordica grosvenori Neogroside
Content be 0.003%.
Embodiment 4
1) volumetric concentration that Momordica grosvenori is added 5 times of its weight be 50% alcohol reflux extract 3 times, every time extract 1h,
Merge extract, filter, take filtrate recycling ethanol, obtain extractum A；
2) extractum A is added upper D201 macroreticular resin after the water dissolves of 15 times of its weight, on solution, column flow rate is 0.3BV/
H, first washes with water to colourless, and the elution flow rate of water is 2BV/h, the ethanol elution being then 75% with volumetric concentration, ethanol
Elution speed is 0.6BV/h, collects ethanol eluate, eluent is reclaimed ethanol, obtains medicinal extract B；
3) medicinal extract B is added the water dissolves of 10 times of its weight, upper D101+ macroreticular resin, on solution, column flow rate is 0.5BV/
H, washes with water, and the elution flow rate of water is 0.5BV/h, collects water elution, concentrates, is dried, obtains final product momorside.Through product
Taste, momorside sweet taste is pure, no return bitter taste.Momorside carried out HPLC analysis, wherein Momordica grosvenori Neogroside
Content is 0.002%.
Claims (9)
1. from Momordica grosvenori extract momorside method it is characterised in that：Comprise the following steps：
1) Momordica grosvenori is added alcohol extract, extract is filtered, take filtrate recycling ethanol, obtain extractum A；
2) upper D201 macroreticular resin after extractum A being dissolved in water, first washes with water to colourless, then uses ethanol elution, collect second
Alcohol eluen, eluent is reclaimed ethanol, obtains medicinal extract B；
3) medicinal extract B is dissolved in water, upper D101+ macroreticular resin, wash with water, collect eluent, concentrate, be dried, obtain final product.
2. according to claim 1 from Momordica grosvenori extract momorside method it is characterised in that：Step 1)
In, the alcohol reflux that Momordica grosvenori is added 5～10 times of its weight extracts 3 times, extracts 1～2h every time, merges extract, filters,
By filtrate recycling ethanol, standby.
3. according to claim 2 from Momordica grosvenori extract momorside method it is characterised in that：Ethanol volume
Concentration is 40～50%.
4. according to claim 1 from Momordica grosvenori extract momorside method it is characterised in that：Step 2)
In, the volumetric concentration of ethanol is 75～80%.
5. according to claim 1 from Momordica grosvenori extract momorside method it is characterised in that：Step 2)
In, the consumption of water is 8～15 times of extractum A weight.
6. according to claim 1 from Momordica grosvenori extract momorside method it is characterised in that：Step 2)
In, on solution, column flow rate is 0.3～0.5BV/h, and the elution flow rate of water is 1～2BV/h, the elution speed of ethanol is 0.5～
0.6BV/h.
7. according to claim 1 from Momordica grosvenori extract momorside method it is characterised in that：Step 3)
In, described D101+ resin is the nonpolar EVA of styrene type, and its technical parameter is：
1) outward appearance：The opaque spherical particle of milky；
2) particle size range 0.3～1.25mm >=95%；
3) water content：60～75%；
4) wet true density：1.05～1.15g/ml；
5) wet apparent density：0.60～0.65g/ml；
6) specific surface area：520～550/g；
7) average pore size：
8) porosity：42～46%；
9) pore volume：1.18～1.24ml/g.
8. according to claim 1 from Momordica grosvenori extract momorside method it is characterised in that：Step 3)
In, the consumption of water is 10～15 times of medicinal extract B weight.
9. according to claim 1 from Momordica grosvenori extract momorside method it is characterised in that：Step 3)
In, on solution, column flow rate is 0.3～0.5BV/h, and the elution flow rate of water is 0.5～0.6BV/h.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108553559A (en) * | 2018-06-22 | 2018-09-21 | 南昌糖友安健康科技有限公司 | A kind of vegetables and fruits extraction composition formula and preparation method for preventing type II diabetes |
| CN108967776A (en) * | 2018-06-27 | 2018-12-11 | 广西驰胜农业科技有限公司 | A kind of fermented beverage and preparation method thereof containing Fructus Monordicae extract |
| CN110339225A (en) * | 2019-07-01 | 2019-10-18 | 上海欧润化妆品有限公司 | A kind of extracting method of Siraitia grosvenorii |
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| CN102408463A (en) * | 2011-11-15 | 2012-04-11 | 威海天泓农业科技有限公司 | Method for extracting mogroside |
| CN105218612A (en) * | 2015-09-30 | 2016-01-06 | 大闽食品（漳州）有限公司 | A kind of method improving Momordica grosvenori mogroside V purity in mogroside |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102408463A (en) * | 2011-11-15 | 2012-04-11 | 威海天泓农业科技有限公司 | Method for extracting mogroside |
| CN105218612A (en) * | 2015-09-30 | 2016-01-06 | 大闽食品（漳州）有限公司 | A kind of method improving Momordica grosvenori mogroside V purity in mogroside |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108553559A (en) * | 2018-06-22 | 2018-09-21 | 南昌糖友安健康科技有限公司 | A kind of vegetables and fruits extraction composition formula and preparation method for preventing type II diabetes |
| CN108553559B (en) * | 2018-06-22 | 2021-03-23 | 南昌糖友安健康科技有限公司 | Formula and preparation method of vegetable and fruit extract for preventing and treating type II diabetes |
| CN108967776A (en) * | 2018-06-27 | 2018-12-11 | 广西驰胜农业科技有限公司 | A kind of fermented beverage and preparation method thereof containing Fructus Monordicae extract |
| CN110339225A (en) * | 2019-07-01 | 2019-10-18 | 上海欧润化妆品有限公司 | A kind of extracting method of Siraitia grosvenorii |
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