CN102408463A

CN102408463A - Extraction method for mogroside

Info

Publication number: CN102408463A
Application number: CN2011103717718A
Authority: CN
Inventors: 毕云英
Original assignee: WEIHAI TIANHONG AGRICULTURAL TECHNOLOGY CO LTD
Current assignee: WEIHAI TIANHONG AGRICULTURAL TECHNOLOGY CO LTD
Priority date: 2011-11-15
Filing date: 2011-11-15
Publication date: 2012-04-11

Abstract

The invention discloses an extraction method for mogroside by taking fructus momordicae as a raw material. The method comprises the following steps of: by utilizing different degrees of capacities of adsorbing other ingredients and the mogroside in fructus momordicae extract by HPD-100 type macroporous resin, loading a sample at a specified speed and washing with ethanol solutions of different concentrations at different elution speeds; combining all eluted remaining liquor; and preparing a mogroside finished product by spray drying. Compared with the prior art in which the extract is subjected to a multiple column-loading adsorption method of adsorption resin and then decolorization resin and/or deodorization resin, the extraction method has the advantages that: a high-purity, decolorized and deodorized mogroside product can be obtained by only an adsorption separation process of strongly polar macroporous adsorption resin without decolorization and deodorization processes. The obtained mogroside has a color which is different from yellowish-brown color and light yellow color of the conventional mogroside, is white or almost white, and has pure sweetness, does not have bitter taste or has the traditional Chinese medicine taste; the content by weight of momordica total glycoside in the mogroside reaches up to over 95 percent; the content by weight of the mogroside V reaches over 65 percent.

Description

A kind of process for extracting of Momordica-Glycosides

Technical field

The present invention relates to the process for extracting of the method for extracting effective components from natural phant, particularly a kind of Momordica-Glycosides.

Background technology

Grosvenor Momordica, the fruit of genus Curcurbitaceae herbaceous perennial vine plant is China's south endemic plant.Grosvenor Momordica is for a long time as Chinese medicine and beverage dry fruit and eat; Its flavor is extremely sweet, cool in nature, nontoxic, go into the spleen lung, have clearing away lung-heat to relieve cough, relax bowel, clearing heat and detoxicating, eliminate the phlegm and enrich blood, beneficial liver, invigorating the spleen, calmness, quench the thirst, promote the stomach function, suppress effects such as asthma and step-down.Since at first reported in 1975 contain diterpene sweet taste glucoside in the Lo Han Guo fruit since; From Lo Han Guo fruit, separated and identified mogroside IV (mogroside IV), mogroside V, mogroside III, mogroside IIE, mogroside IIIE, mogroside VI, mogroside A, the new glucoside of Grosvenor Momordica 11 kinds of cucurbitane Triterpene Glycosides compounds such as (nemogroside); This compounds is the main active substances in the Lo Han Guo fruit; Accounting for dry fruit and weigh 4%, except that minority is no sweet (like mogroside IV) or bitter taste (like the new glucoside of Grosvenor Momordica) material, is sweet ingredient or little sweet substance mostly; Wherein mogroside V is that triterpene is the strong sweet substance of glucoside S-5; Being content and all higher composition of sugariness in the Lo Han Guo fruit, is main sweet ingredient, and its sugariness is equivalent to 300 times of sucrose.Momordica-Glycosides flavor is sweet and pure, free from extraneous odour, and the aftertaste sense is strong, and Heat stability is good can be used as the sweeting agent of food, dish, is diabetics's ideal sugar surrogate.

At present, from Grosvenor Momordica, extract the Momordica-Glycosides method and generally be with dry fruit or to give birth to fruit be raw material, with organic solvent or water extraction, extracting solution separates with resin absorption, wash-out, concentrate, dry; Or be raw material with the fresh Fructus Momordicae, adopt the enzyme extraction technology, obtain Momordica-Glycosides.Separate though this method only needs once to cross post, also need just to take off flavor with deodorization agent in addition again and handle, so its operation is still more, complicated operation, cost are also corresponding higher.

Summary of the invention

The objective of the invention is to deficiency, a kind of method of using Grosvenor Momordica as the feedstock production Momordica-Glycosides is provided to prior art.

The objective of the invention is to realize through following technical scheme: a kind of method of using Grosvenor Momordica as the feedstock production Momordica-Glycosides may further comprise the steps:

Step 1, the Grosvenor Momordica crude drug is through section or be ground into meal, and the volume percent that adds 8～10 times of volumes is that 60% aqueous ethanolic solution extracts each 1.5～2 hours 2～3 times;

Step 2, liquid behind the united extraction, centrifuging, filtrate decompression concentrates and reclaims ethanol to relative density of medicine liquid is 1.05;

Step 3, after the soup after concentrating is diluted to an appearance soup A440 and reaches 60 with deionized water, last HPD-100 type macroporous resin column, kind speed is at 5～6 times upper prop macroporous resin volume per hour in the control, and applied sample amount and upper prop macroporous resin volume ratio are 1: 1; Soup behind the collection upper prop; Behind the deionized water of one times of upper prop macroporous resin of soup wash-out volume; To use volume percent be 25% aqueous ethanolic solution wash-out to elutriant A238 less than 0.8, collect elutriant, using volume percent then is 70% aqueous ethanolic solution wash-out elutriant;

Step 4 remains soup after merging all wash-outs, after concentrating under reduced pressure reclaims ethanol, and spray-dried Momordica-Glycosides.

The present invention gets beneficial effect: whole technological process only makes water and ethanol for extracting solvent; Production process does not have waste water, waste gas to produce; Used HPD-100 type macroporous resin and ethanol reagent are all recyclable to be utilized again; Being fit to suitability for industrialized production, is a kind of environment-friendly type new technology, obtains high-purity natural product Momordica-Glycosides.

Embodiment

Embodiment 1

10 kilograms of bright medicinal materials of Grosvenor Momordica get soup 3140ml after 60% ethanol (by volume) extraction concentrates; Get 1750ml soup (containing Momordica-Glycosides 93537.5mg) and be diluted to upward 1500mlHPD-100 macroporous resin of A440=60 with deionized water; (the about 60000ml of the medicine liquid volume that loses shape contains Momordica-Glycosides 58464mg in the soup behind the upper prop, account for soup Momordica-Glycosides total content 62.5%); After last appearance finishes; Earlier with deionized water wash-out resin absorption water-soluble impurity, (water lotion contains Momordica-Glycosides 4209.75mg) used Momordica-Glycosides that 25% ethanol (by volume) washes resin absorption again (elutriant contains Momordica-Glycosides glycosides 22478.38mg, accounts for 24% of total content in the soup less than 0.8 to elutriant A238; So far about about 91% Grosvenor Momordica general glycoside separates from soup); Then with 70% ethanol elution residue effective constituent, remain soup after merging all wash-outs, after spray-dried must Momordica-Glycosides about 44.8g; Wherein Grosvenor Momordica general glycoside weight content is up to more than 95%, and the Momordica grosvenori mogroside V weight content reaches more than 65%.

Claims

1. the process for extracting of a Momordica-Glycosides is characterized in that, may further comprise the steps:

(1) the Grosvenor Momordica crude drug adds 8～10 times of volume of ethanol extraction with aqueous solution 2～3 times, each 1.5～2 hours through cutting into slices or being ground into meal;

(2) liquid behind the united extraction, centrifuging, filtrate decompression concentrates and reclaims ethanol to relative density of medicine liquid is 1.05;

(3) after the soup after will concentrating is diluted to an appearance soup A440 and reaches 60 with deionized water, last HPD-100 type macroporous resin column, kind speed is at 5～6 times upper prop macroporous resin volume per hour in the control, and applied sample amount and upper prop macroporous resin volume ratio are 1: 1; Soup behind the collection upper prop; Behind the deionized water of one times of upper prop macroporous resin of soup wash-out volume; To use volume percent be 25% aqueous ethanolic solution wash-out to elutriant A238 less than 0.8, collect elutriant, using volume percent then is 70% aqueous ethanolic solution wash-out elutriant;

(4) remain soup after merging all wash-outs, after concentrating under reduced pressure reclaims ethanol, spray-dried Momordica-Glycosides.

2. the method for using Grosvenor Momordica as the feedstock production Momordica-Glycosides according to claim 1 is characterized in that, in the said step (1), the concentration of volume percent in the aqueous ethanolic solution is 60%.