CN109289242A - Combine the method for preparing Fructus Momordicae fructoside V using subcritical and molecular distillation - Google Patents
Combine the method for preparing Fructus Momordicae fructoside V using subcritical and molecular distillation Download PDFInfo
- Publication number
- CN109289242A CN109289242A CN201811085219.0A CN201811085219A CN109289242A CN 109289242 A CN109289242 A CN 109289242A CN 201811085219 A CN201811085219 A CN 201811085219A CN 109289242 A CN109289242 A CN 109289242A
- Authority
- CN
- China
- Prior art keywords
- subcritical
- molecular distillation
- fructus momordicae
- momordicae fructoside
- siraitia grosvenorii
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D3/00—Distillation or related exchange processes in which liquids are contacted with gaseous media, e.g. stripping
- B01D3/12—Molecular distillation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J17/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, having an oxygen-containing hetero ring not condensed with the cyclopenta(a)hydrophenanthrene skeleton
- C07J17/005—Glycosides
Abstract
Combine the method for preparing Fructus Momordicae fructoside V using subcritical and molecular distillation the present invention relates to a kind of comprising following steps: 1) carrying out subcritical abstraction for Grosvenor Momordica Fruit, obtains Siraitia grosvenorii extract；2) Siraitia grosvenorii extract is subjected to molecular distillation, obtains the light component of the V containing Fructus Momordicae fructoside.When step 1) Subcritical extracts, temperature is 10-50 DEG C, and pressure is 0.5-2.0 MPa, and the time is 3-5 h.The method of the present invention includes three raw material comminution pretreatment, subcritical abstraction and molecular distillation steps, and operating procedure is simple and convenient, time-consuming shorter, is suitble to large-scale production and application；Temperature is lower in entire technical process to destroy fragrance matter, smaller on target compound influence, and raw material has no introducing harmful substance from nature during the preparation process, and toxic solvent residue problem is not present；Prepare that resulting Fructus Momordicae fructoside V purity is higher, and purity is 94.6% or more using the method for the present invention.
Description
Technical field
This patent belongs to active ingredients in natural plants extractive technique field, and in particular to a kind of to utilize subcritical and molecule
The method for preparing natural sweetener Fructus Momordicae fructoside V is combined in distillation.
Background technique
Siraitia grosvenorii is the perennial liana of Curcurbitaceae, and leaf is heart-shaped, dioecism, summer-flowering, seed in autumn.Chinese medicine
Lung heat phlegm-fire cough, pharynx are cured mainly containing medicinal ingredients such as Fructus Momordicae fructoside, a variety of amino acid and vitamins with its fruit medicine
Laryngitis, tonsillitis, acute gastritis, constipation etc. are the famous specialties in Guangxi China Guilin City.
Glucosides rich in Siraitia grosvenorii, total content is 3.78-3.86% in dry fruit, and content is most in glucosides
It is glucosides V, content is 22% or so of total glucosides.The sugariness of glucosides V is 256 times of sucrose, and sweet and pure, free from extraneous odour returns
Sense of taste is strong, and thermal stability is good, can be used as the sweetener of food, dish, and heat is lower is adiposis patient and is unsuitable for using confectionery
The ideal sugar substitute of diabetes patient, and have effects that eliminating phlegm and relieving cough is relievingd asthma.
The extraction of existing Fructus Momordicae fructoside V, preparation method are more, but resulting Fructus Momordicae fructoside V purity is not high or operates
Process is complicated, takes a long time.Such as, Chinese patent CN200810073876.3 discloses the preparation method of Fructus Momordicae fructoside V, is
Separating-purifying, obtained final products are carried out to the thick glycosides containing Fructus Momordicae fructoside V by silica gel column chromatography and C18 reversed phase chromatography
Purity only 70% or more, this method prepare Fructus Momordicae fructoside V there are complex steps, product purity is lower the defects of.In in addition,
State patent CN200910044558.9 discloses a kind of production method of fructus of the Mogroside V content greater than 60%,
Mainly using saccharification enzymatic hydrolysis, water are mentioned, are concentrated, ion-exchange resin decolorization, macroporous absorbent resin separate and alumina column chromatography
Purification, and finished product is obtained through last concentrate drying.Chromatography of this method Jing Guo multistep, equally exist operating process it is cumbersome, consumption
When it is longer the defects of.
Summary of the invention
Present invention aims to overcome that prior art defect, provide it is a kind of combined using subcritical and molecular distillation prepare sieve
The method of Chinese fructoside V, this method step is easy, and the purity of preparation gained Fructus Momordicae fructoside V is higher.
To achieve the above object, the present invention adopts the following technical scheme:
A method of combined using subcritical and molecular distillation and prepare Fructus Momordicae fructoside V comprising following steps:
1) Grosvenor Momordica Fruit is subjected to subcritical abstraction, obtains Siraitia grosvenorii extract；
2) Siraitia grosvenorii extract is subjected to molecular distillation, obtains the light component of the V containing Fructus Momordicae fructoside.
Specifically, when step 1) Subcritical extracts, temperature is 10-50 DEG C, pressure in order to obtain preferable effect of extracting
Power is 0.5-2.0 MPa, and the time is 3-5 h.
Further, when step 1) Subcritical extracts, extractant is one of normal butane, propane, dimethyl ether, ether
Or the mixture of two or more arbitrary proportions, Grosvenor Momordica Fruit and extractant is added by solid-liquid ratio 1g:6-15mL when extraction.
Specifically, in order to obtain preferable molecular distillation effect, when carrying out molecular distillation in step 2, vapo(u)rizing temperature 50
± 5 DEG C, distillation pressure is 0.01-0.1 Pa.Further in order to obtain higher product purity, preferably molecule steaming is being carried out
Before evaporating, Siraitia grosvenorii extract is concentrated under reduced pressure in advance to remove part extractant, the partial extraction agent of removing is to account for extraction
The 50-70% of agent totality accumulated amount is advisable.
Further, when carrying out molecular distillation in step 2, the revolving speed of scraper plate is 50-70 rad/min, charging rate
0.1-1.0mL/min.
It is further preferred that Grosvenor Momordica Fruit is preparatory in step 1) crosses 40-60 mesh screen.Specifically, Siraitia grosvenorii is ground
Alms bowl is pulverized, is sieved (aperture of sieve is 40-60 mesh), and Grosvenor Momordica Fruit is obtained.
Subcritical fluid extraction technology is the special nature using subcritical fluids, and material injects subcritical in extractor
Fluid impregnates, the extraction process carried out under the conditions of certain solid-liquid ratio, extraction temperature, extraction time, extracting pressure.Extraction is mixed
It closes liquid and enters vapo(u)rization system after being separated by solid-liquid separation, it, will according to the principle of reduction vaporization under the action of compressor and vacuum pump
Extractant switchs to gaseous state by liquid to obtain Objective extraction object.
Molecular distillation is also known as short-path distillation, is a kind of new and high technology of liquid-liquid separation, it is different from Conventional espresso by boiling
The poor separation principle of point, but separation is realized by the difference of different material molecular tools, molecular distillation technique is in spy
Enrichment, the isolation technics of flavor component etc. application are levied than wide.
The method of the present invention passes through raw material comminution pretreatment, subcritical abstraction and molecular distillation three using Siraitia grosvenorii as raw material
Fructus Momordicae fructoside V is prepared in step.It is detected through efficient liquid phase, Fructus Momordicae fructoside V in molecular distillation light component obtained
Purity reaches 94.6% or more.Compared to the prior art, the method for the present invention has the beneficial effect that:
1) the method for the present invention includes three raw material comminution pretreatment, subcritical abstraction and molecular distillation steps, and operating procedure is simple
It is convenient, it is time-consuming shorter, it is suitble to large-scale production and application；Temperature is lower in entire technical process to carry out brokenly fragrance matter
It is bad, it is smaller on target compound influence, and raw material has no introducing harmful substance, no from nature during the preparation process
There are toxic problem of solvent residual；
2) prepare that resulting Fructus Momordicae fructoside V purity is higher, and purity is 94.6% or more using the method for the present invention.
Detailed description of the invention
Fig. 1 is the HPLC spectrogram that embodiment 1 prepares gained Fructus Momordicae fructoside V finished product.
Specific embodiment
Technical solution of the present invention is further discussed in detail with reference to embodiments, but protection scope of the present invention
It is not limited thereto.
Embodiment 1
A method of combined using subcritical and molecular distillation and prepare Fructus Momordicae fructoside V comprising following steps:
1) Siraitia grosvenorii is pulverized with stone roller alms bowl, crosses 40-60 mesh screen, obtain Grosvenor Momordica Fruit；
2) it takes 200 g of Grosvenor Momordica Fruit to be placed in subcritical abstraction kettle, the positive fourth of volume ratio 1:1 is added into subcritical abstraction kettle
Alkane and 1200 mL of dimethyl ether extract 3 under conditions of extraction temperature is 10 DEG C, extracting pressure is 2.0 MPa as extractant
H obtains Siraitia grosvenorii extract；
3) Siraitia grosvenorii extract is concentrated under reduced pressure to remove 60% extractant, then vapo(u)rizing temperature is 50 DEG C, distillation pressure is
0.01 Pa, scraper plate revolving speed are 50 rad/min, charging rate carries out molecular distillation under conditions of being 0.1 mL/min, are contained
The light component of Fructus Momordicae fructoside V, then freeze-dried machine are freeze-dried 24 h, obtain white powder crystal, as Siraitia grosvenorii
Glucosides V finished product, yield 13.65%.
Fructus Momordicae fructoside V finished product obtained by above-mentioned preparation is carried out efficient liquid phase detection, testing result discovery: purity is
96.95%, the result is shown in Figure 1.
The actual conditions of efficient liquid phase detection are as follows:
Chromatographic condition: chromatographic column Agilent ZORBAX SB-C18(4.6mm × 250nm, 5 μm)；Mobile phase water: (A) and second
Nitrile (B), using gradient elution, 0-40 min, 5%-68% B；30 DEG C of column temperature；203 nm of Detection wavelength；Volume flow 1mL/
min。
Embodiment 2
A method of combined using subcritical and molecular distillation and prepare Fructus Momordicae fructoside V comprising following steps:
1) Siraitia grosvenorii is pulverized with stone roller alms bowl, crosses 40-60 mesh screen, obtain Grosvenor Momordica Fruit；
2) it takes 200 g of Grosvenor Momordica Fruit to be placed in subcritical abstraction kettle, 2400 mL conduct of propane is added into subcritical abstraction kettle
Extractant extracts 4 h under conditions of extraction temperature is 30 DEG C, extracting pressure is 1.5 MPa, obtains Siraitia grosvenorii extract；
3) Siraitia grosvenorii extract is concentrated under reduced pressure to remove 60% extractant, then vapo(u)rizing temperature is 50 DEG C, distillation pressure is
0.03 Pa, scraper plate revolving speed are 60 rad/min, charging rate carries out molecular distillation under conditions of being 0.3 mL/min, are contained
The light component of Fructus Momordicae fructoside V, then freeze-dried machine are freeze-dried 24 h, obtain white powder crystal, as Siraitia grosvenorii
Glucosides V finished product, yield 12.37%.
Fructus Momordicae fructoside V finished product obtained by above-mentioned preparation is carried out efficient liquid phase detection, testing result discovery: purity is
95.81%。
Embodiment 3
A method of combined using subcritical and molecular distillation and prepare Fructus Momordicae fructoside V comprising following steps:
1) Siraitia grosvenorii is pulverized with stone roller alms bowl, crosses 40-60 mesh screen, obtain Grosvenor Momordica Fruit；
2) it takes 200 g of Grosvenor Momordica Fruit to be placed in subcritical abstraction kettle, the propane of volume ratio 1:1 is added into subcritical abstraction kettle
With 2000 mL of ether as extractant, 3 h are extracted under conditions of extraction temperature is 40 DEG C, extracting pressure is 1.0 MPa, are obtained
To Siraitia grosvenorii extract；
3) Siraitia grosvenorii extract is concentrated under reduced pressure to remove 60% extractant, then vapo(u)rizing temperature is 50 DEG C, distillation pressure is
0.06 Pa, scraper plate revolving speed are 55 rad/min, charging rate carries out molecular distillation under conditions of being 0.6 mL/min, are contained
The light component of Fructus Momordicae fructoside V, then freeze-dried machine are freeze-dried 24 h, obtain white powder crystal, as Siraitia grosvenorii
Glucosides V finished product, yield 14.46%.
Fructus Momordicae fructoside V finished product obtained by above-mentioned preparation is carried out efficient liquid phase detection, testing result discovery: purity is
94.62%。
Embodiment 4
A method of combined using subcritical and molecular distillation and prepare Fructus Momordicae fructoside V comprising following steps:
1) Siraitia grosvenorii is pulverized with stone roller alms bowl, crosses 40-60 mesh screen, obtain Grosvenor Momordica Fruit；
2) it takes 200 g of Grosvenor Momordica Fruit to be placed in subcritical abstraction kettle, normal butane and ether (body is added into subcritical abstraction kettle
Product is than 1:1) 3000 mL as extractant, extract 5 h under conditions of extraction temperature is 50 DEG C, extracting pressure is 2.0 MPa,
Obtain Siraitia grosvenorii extract；
3) Siraitia grosvenorii extract is concentrated under reduced pressure to remove 60% extractant, then vapo(u)rizing temperature is 50 DEG C, distillation pressure is
0.1 Pa, scraper plate revolving speed are 70 rad/min, charging rate carries out molecular distillation under conditions of being 1.0 mL/min, are contained
The light component of Fructus Momordicae fructoside V, then freeze-dried machine are freeze-dried 24 h, obtain white powder crystal, as Siraitia grosvenorii
Glucosides V finished product, yield 12.98%.
Fructus Momordicae fructoside V finished product obtained by above-mentioned preparation is carried out efficient liquid phase detection, testing result discovery: purity is
97.82%。
Embodiment 5
A method of combined using subcritical and molecular distillation and prepare Fructus Momordicae fructoside V comprising following steps:
1) Siraitia grosvenorii is pulverized with stone roller alms bowl, crosses 40-60 mesh screen, obtain Grosvenor Momordica Fruit；
2) it takes 200 g of Grosvenor Momordica Fruit to be placed in subcritical abstraction kettle, ether and dimethyl ether (body is added into subcritical abstraction kettle
Product is than 1:1) 2800 mL as extractant, extract 4 h under conditions of extraction temperature is 35 DEG C, extracting pressure is 1.5 MPa,
Obtain Siraitia grosvenorii extract；
3) Siraitia grosvenorii extract is concentrated under reduced pressure to remove 60% extractant, then vapo(u)rizing temperature is 50 DEG C, distillation pressure is
0.07 Pa, scraper plate revolving speed are 60 rad/min, charging rate carries out molecular distillation under conditions of being 0.04 mL/min, are obtained
The light component of the V containing Fructus Momordicae fructoside, then freeze-dried machine are freeze-dried 24 h, obtain white powder crystal, as arhat
Fructoside V finished product, yield 14.41%.
Fructus Momordicae fructoside V finished product obtained by above-mentioned preparation is carried out efficient liquid phase detection, testing result discovery: purity is
95.89%。
Embodiment 6
A method of combined using subcritical and molecular distillation and prepare Fructus Momordicae fructoside V comprising following steps:
1) Siraitia grosvenorii is pulverized with stone roller alms bowl, crosses 40-60 mesh screen, obtain Grosvenor Momordica Fruit；
2) Grosvenor Momordica Fruit is taken to be placed in subcritical abstraction kettle, be added into subcritical abstraction kettle butane and propane (volume ratio 1:
1) 2200mL is as extractant, and under conditions of extraction temperature is 25 DEG C, extracting pressure is 2.0 MPa, 3 h of extraction obtain arhat
Fruit extract；
3) Siraitia grosvenorii extract is concentrated under reduced pressure to remove 60% extractant, then vapo(u)rizing temperature is 50 DEG C, distillation pressure is
0.03 Pa, scraper plate revolving speed are 65 rad/min, charging rate carries out molecular distillation under conditions of being 0.08 mL/min, are obtained
The light component of V containing Fructus Momordicae fructoside, then freeze-dried machine are freeze-dried 24 h, obtain white powder crystal, as Siraitia grosvenorii
Glucosides V finished product, yield 12.69%.
Fructus Momordicae fructoside V finished product obtained by above-mentioned preparation is carried out efficient liquid phase detection, testing result discovery: purity is
97.83%。
Claims (6)
1. a kind of combine the method for preparing Fructus Momordicae fructoside V using subcritical and molecular distillation, which is characterized in that including walking as follows
It is rapid: 1) Grosvenor Momordica Fruit to be subjected to subcritical abstraction, obtain Siraitia grosvenorii extract；
2) Siraitia grosvenorii extract is subjected to molecular distillation, obtains the light component of the V containing Fructus Momordicae fructoside.
2. combining the method for preparing Fructus Momordicae fructoside V using subcritical and molecular distillation as described in claim 1, feature exists
In when step 1) Subcritical extracts, temperature is 10-50 DEG C, and pressure is 0.5-2.0 MPa, and the time is 3-5 h.
3. combining the method for preparing Fructus Momordicae fructoside V using subcritical and molecular distillation as described in claim 1, feature exists
In when step 1) Subcritical extracts, extractant is that one or more of normal butane, propane, dimethyl ether, ether are any
Grosvenor Momordica Fruit and extractant is added by solid-liquid ratio 1g:6-15mL when extraction in the mixture of ratio.
4. combining the method for preparing Fructus Momordicae fructoside V using subcritical and molecular distillation as described in claim 1, feature exists
In when carrying out molecular distillation in step 2, vapo(u)rizing temperature is 50 ± 5 DEG C, and distillation pressure is 0.01-0.1 Pa.
5. combining the method for preparing Fructus Momordicae fructoside V using subcritical and molecular distillation as described in claim 1, feature exists
In when carrying out molecular distillation in step 2, the revolving speed of scraper plate is 50-70 rad/min, charging rate 0.1-1.0mL/min.
6. combine the method for preparing Fructus Momordicae fructoside V using subcritical and molecular distillation as described in claim 1 to 5 is any,
It is characterized in that, Grosvenor Momordica Fruit is preparatory in step 1) crosses 40-60 mesh screen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811085219.0A CN109289242A (en) | 2018-09-18 | 2018-09-18 | Combine the method for preparing Fructus Momordicae fructoside V using subcritical and molecular distillation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811085219.0A CN109289242A (en) | 2018-09-18 | 2018-09-18 | Combine the method for preparing Fructus Momordicae fructoside V using subcritical and molecular distillation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109289242A true CN109289242A (en) | 2019-02-01 |
Family
ID=65163456
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811085219.0A Pending CN109289242A (en) | 2018-09-18 | 2018-09-18 | Combine the method for preparing Fructus Momordicae fructoside V using subcritical and molecular distillation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109289242A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113603742A (en) * | 2021-08-06 | 2021-11-05 | 湖南华诚生物资源股份有限公司 | Preparation method of mogroside V |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102531982A (en) * | 2011-12-23 | 2012-07-04 | 安徽绿能技术研究院 | Method for extracting high purity allicin and device thereof |
| CN105132184A (en) * | 2015-09-11 | 2015-12-09 | 中国林业科学研究院林产化学工业研究所 | Method for preparing gardenia essence by subcritical extraction and molecular distillation coupling |
| CN106749172A (en) * | 2016-11-22 | 2017-05-31 | 中国烟草总公司郑州烟草研究院 | A kind of method that utilization subcritical abstraction molecular clock GC-MS purifies nicotine |
| CN106978252A (en) * | 2017-05-24 | 2017-07-25 | 中国农业大学 | A kind of method that pumpkin sterol is extracted based on subcritical fluids and molecular distillation |
| CN107033626A (en) * | 2017-04-28 | 2017-08-11 | 常德华馥生物技术有限公司 | A kind of method that subcritical abstraction is combined separate tobacco natural pigment with molecular distillation |
| CN107129865A (en) * | 2017-05-24 | 2017-09-05 | 中国农业大学 | A kind of method that pumpkin phylloquinone is extracted based on subcritical fluids and molecular distillation |
| CN107996840A (en) * | 2018-01-30 | 2018-05-08 | 灵川县億旺养殖专业合作社 | A kind of feed addictive used for early pregnancy sow |
| CN108276465A (en) * | 2017-03-14 | 2018-07-13 | 桂林莱茵生物科技股份有限公司 | A method of isolating and purifying mogroside V with subcritical water desorption techniques |
-
2018
- 2018-09-18 CN CN201811085219.0A patent/CN109289242A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102531982A (en) * | 2011-12-23 | 2012-07-04 | 安徽绿能技术研究院 | Method for extracting high purity allicin and device thereof |
| CN105132184A (en) * | 2015-09-11 | 2015-12-09 | 中国林业科学研究院林产化学工业研究所 | Method for preparing gardenia essence by subcritical extraction and molecular distillation coupling |
| CN106749172A (en) * | 2016-11-22 | 2017-05-31 | 中国烟草总公司郑州烟草研究院 | A kind of method that utilization subcritical abstraction molecular clock GC-MS purifies nicotine |
| CN108276465A (en) * | 2017-03-14 | 2018-07-13 | 桂林莱茵生物科技股份有限公司 | A method of isolating and purifying mogroside V with subcritical water desorption techniques |
| CN107033626A (en) * | 2017-04-28 | 2017-08-11 | 常德华馥生物技术有限公司 | A kind of method that subcritical abstraction is combined separate tobacco natural pigment with molecular distillation |
| CN106978252A (en) * | 2017-05-24 | 2017-07-25 | 中国农业大学 | A kind of method that pumpkin sterol is extracted based on subcritical fluids and molecular distillation |
| CN107129865A (en) * | 2017-05-24 | 2017-09-05 | 中国农业大学 | A kind of method that pumpkin phylloquinone is extracted based on subcritical fluids and molecular distillation |
| CN107996840A (en) * | 2018-01-30 | 2018-05-08 | 灵川县億旺养殖专业合作社 | A kind of feed addictive used for early pregnancy sow |
Non-Patent Citations (1)
| Title |
|---|
| 罗杨合: "《化学分离分析方法与技术研究》", 31 January 2014, 中国商务出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113603742A (en) * | 2021-08-06 | 2021-11-05 | 湖南华诚生物资源股份有限公司 | Preparation method of mogroside V |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101386636B (en) | Mogroside extraction method from grosvenor momordica | |
| CN106220698A (en) | A kind of method of separating high-purity Hesperidin, neohesperidin, naringin and Neosynephrine from Fructus Aurantii Immaturus | |
| KR102580703B1 (en) | Stevia rebaudiana industrialized method for simultaneously producing chlorogenic acid and stevioside | |
| CN102351824B (en) | Method for preparing lactuca indica and lactucin | |
| CN102408463A (en) | Extraction method for mogroside | |
| WO2020063894A1 (en) | Industrial utilization method for stevia rebaudiana and stevioside and chlorogenic acid of stevia rebaudiana | |
| CN105132172B (en) | A method of preparing tobacco orrisroot Flavonoid substances from orrisroot | |
| CN102675398B (en) | A kind of method extracting momordica grosvenori glycoside V and farnesol from Grosvenor Momordica | |
| CN108276465A (en) | A method of isolating and purifying mogroside V with subcritical water desorption techniques | |
| CN101407535B (en) | Preparation of high-purity Momordica grosvenori mogroside V | |
| CN103446195B (en) | The preparation method of Folium Ginkgo extract | |
| CN110105458A (en) | The method that polysaccharide and PEARLITOL 25C are extracted in waste liquid is extracted from mogroside | |
| CN104262446B (en) | The method of mogroside Ⅴ is extracted from Grosvenor Momordica | |
| CN104530145A (en) | Method for extracting high-purity persimmon tannin from persimmon fruits | |
| CN109879919B (en) | Method for separating and preparing three flavonoid glycosides from spina date seeds | |
| CN110845566A (en) | Dammarane triterpene glycoside compound and preparation method and application thereof | |
| CN104892717B (en) | A kind of technical grade preparative liquid chromatography separation method of momordica glycoside V | |
| CN109289242A (en) | Combine the method for preparing Fructus Momordicae fructoside V using subcritical and molecular distillation | |
| CN109021046A (en) | A method of extracting quercitin and mountain naphthalene glycosides simultaneously from Siraitia grosvenorii cauline leaf | |
| CN108864238A (en) | The method that arhat fruit seed oil and cupreol are extracted in slag is extracted from Siraitia grosvenorii | |
| CN104606288A (en) | Novel method for preparing total flavonoid aglycone extract of scutellaria baicalensis georgi | |
| KR20210074526A (en) | A high efficiency extraction method of phyllodulcin from Hydrangea serrata using supercritical extraction | |
| CN113603742B (en) | Preparation method of mogroside V | |
| CN109293726A (en) | Diol type ginsenoside extract and preparation method thereof | |
| CN113429444B (en) | Method for separating and purifying rubusoside from stevia rebaudiana mother liquor sugar |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190201 |
|
| RJ01 | Rejection of invention patent application after publication |