CN108464963B

CN108464963B - Composition for oral cavity and application and preparation method thereof

Info

Publication number: CN108464963B
Application number: CN201810326337.XA
Authority: CN
Inventors: 郭智华; 徐双华; 李宏杰; 尚林晏
Original assignee: Holwray Pharmaceutical China Co ltd
Current assignee: Holwray Pharmaceutical China Co ltd
Priority date: 2018-04-12
Filing date: 2018-04-12
Publication date: 2020-06-16
Anticipated expiration: 2038-04-12
Also published as: CN108464963A

Abstract

The invention relates to a composition for oral cavity and application and a preparation method thereof. The composition comprises, by weight, 0.1-1 part of lemon fruit extract, 0.1-2 parts of propolis extract, 0.05-0.8 part of tea extract, 0.5-5 parts of sea salt, 0.1-5 parts of aloe vera extract, 0.05-0.8 part of alternate leaf cajeput essential oil, 0.06-1 part of white birch bark extract and 0.02-1 part of magnolia bark extract. The composition provided by the invention is combined by screening components, utilizing the medicinal mechanism of different components and combining with a specific proportion, and fully utilizing the synergistic effect among the components, so that the obtained composition has the effects of efficiently inhibiting bacteria, eliminating helicobacter pylori, relieving gingival inflammation and removing halitosis. Meanwhile, the composition provided by the invention is reasonable in cost, simple and convenient to prepare, safe to use, strong in universality and more suitable for commercial popularization.

Description

Composition for oral cavity and application and preparation method thereof

Technical Field

The invention relates to the field of daily chemicals, in particular to a composition for an oral cavity and an application and a preparation method thereof.

Background

The oral cavity is an important part of the body, is in direct contact with the outside, has proper temperature and humidity, and also has food residues and aged and necrotic cells as a nutrient source of bacteria, so that a large amount of bacteria including cocci, bacilli, fungi, etc. must exist in the oral cavity. Bacteria exist in the oral cavity of the infant just after birth, and are contained in oral mucosa, teeth and saliva, so that the bacteria can not cause diseases under normal conditions, but can help prevent other pathogenic bacteria from growing and breeding in the oral cavity, and the occurrence of oral diseases is reduced. However, if the oral hygiene conditions are poor and the body resistance is reduced, the types of bacteria are disordered, and pathogenic bacteria overgrow. Growth of e.g. lactobacilli and streptococcus mutans let us grow decayed teeth; the fusiform bacillus and the actinomyces viscosus can cause gum inflammation, bleeding and swelling and pain; bacteroides melanoides, carbon dioxide phagemids and some actinomycetes are liable to cause periodontitis, resulting in gingival pus discharge, tooth loosening and the like.

The oral cavity can be kept in a healthy state only if good ecological balance is kept among microorganisms in the oral cavity and between the microorganisms and host oral cavity tissues. If the proportion of individual or a plurality of microbes inherent in the oral cavity micro-ecology is obviously increased to become the advantage of a colonized part (especially the proportion of pathogenic bacteria) due to the influence of external microbes or other factors, the original oral cavity micro-ecology balance is broken, and flora imbalance can occur to cause oral diseases. Common oral diseases are: caries, periodontitis, gingivitis, halitosis, etc.

Helicobacter pylori is a chronic infectious pathogen with a high infection rate, and Hp infection is an infectious disease, without a "carry" state. Since Krajden equals to the first success of separating and culturing Hp from dental plaque of a gastritis patient in 1989, as for Hp in the oral cavity, students in the oral cavity use various methods such as bacterial culture, Polymerase Chain Reaction (PCR), immunological detection and the like to confirm the existence of Hp in the oral cavity, further research shows that the Hp in the oral cavity and Hp in the stomach have certain correlation, and the oral cavity is proposed to be another important gathering place of Hp. And the detection rate of Hp in the oral cavity is positively correlated with the severity and the activity of the periodontal disease. In recent years, the eradication rate of the stomach Hp is continuously reduced, gastroduodenal diseases are easy to relapse, and Hp reinfection causes troubles to doctors and patients.

The types of halitosis can be divided into physiological halitosis and pathological halitosis. Physiological halitosis is caused by that oral hygiene is not noticed in daily life, food residues in the mouth are accumulated for a long time, and are fermented, decayed and decomposed under the action of bacteria to generate substances such as indole sulfhydryl and amine, and the like, so that decayed malodor is generated. Physiological bad breath can be removed by enhancing oral hygiene without special treatment. The pathological halitosis can be caused by oral cavity problems such as dental plaque, dental caries and the like on one hand, and can be caused by medical diseases such as acute and chronic gastritis, sour and stink generated by peptic ulcer and the like on the other hand. The infection of helicobacter pylori is the important cause of acute and chronic gastritis. Helicobacter pylori can enter from the mouth, deposit in the oral cavity, enter the stomach through swallowing action, colonize and infect after reaching gastric mucosa, cause chronic and superficial gastritis for weeks or months, and develop into duodenal ulcer, gastric ulcer, lymphoproliferative gastric lymphoma, chronic atrophic gastritis and the like after years or decades, and the latter is the most dangerous factor causing gastric cancer. And after stomach diseases appear, the halitosis problem can be caused. Therefore, the treatment of pathological halitosis needs to be started from oral diseases and medical diseases, and the treatment is carried out fundamentally.

Gingivitis is the most common disease of the heel, of which plaque gingivitis is the predominant chronic infectious disease. Plaque gingivitis is primarily caused by the accumulation of plaque that can be aggravated by other local factors such as tartar, poor restorations, food-packed tooth crowding, mouth breathing, etc. of microorganisms that accumulate in the plaque in the neck and gingival sulcus. The diagnosis and treatment of the disease are simple, and the prognosis is good. However, the disease has high prevalence rate, and if oral hygiene control is not good after healing, the disease is easy to relapse, and a part of patients can also develop periodontitis, so that prevention of occurrence and relapse of gingivitis is very important.

The problems bring certain troubles to the health and life of people and have adverse effects on our life.

Disclosure of Invention

In order to solve the above technical problems, the present invention provides an oral product which can not only kill pathogenic bacteria in the oral cavity including helicobacter pylori, but also alleviate gingival inflammation and eliminate halitosis.

The invention is realized by adopting the following technical scheme.

A composition for use in the oral cavity comprising the following components:

the present inventors have surprisingly found that the combination of the above ingredients in specific proportions provides a composition with superior efficacy to other proportions or combinations of other ingredients, indicating a synergistic effect between the above ingredients.

Preferably, the composition comprises the following components in parts by weight:

in the present invention, the above components can be prepared into extracts according to the conventional operation in the field, and only the preferable scheme is proposed as follows:

the lemon fruit extract is an aqueous extract of lemon fruit; the lemon fruit extract is prepared by washing, drying and crushing a lemon fruit medicinal material, extracting with water, concentrating, and spray-drying liquid. Has antibacterial and dental caries preventing effects.

The propolis extract is alcohol extract of propolis, and has strong antibacterial effect.

The tea extract is alcohol extract of tea; the tea is washed, dried, crushed, alcohol-extracted, concentrated, liquid spray-dried and made into tea extract. Has hemostatic, antibacterial, halitosis removing, and heat and toxic materials clearing away effects.

The sea salt has whitening and antibacterial effects.

The Aloe vera extract is a lyophilized extract of Aloe vera; has antibacterial, antiinflammatory, and wound healing effects. Can help resist diseases around teeth and protect other environment in oral cavity. Has remarkable rehabilitation ability, and can eliminate edema and relieve pain of patients.

The melaleuca alternifolia essential oil is prepared by steam distillation, has broad-spectrum antibacterial, antifungal, antiviral and anti-inflammatory activities, and has strong antibacterial performance on escherichia coli, staphylococcus aureus, staphylococcus epidermidis, escherichia coli, pseudomonas aeruginosa, candida albicans and the like. In recent years, the method has been widely used for preparing skin external preparations, disinfectants, and perfumes and preservatives in cosmetics.

The cortex Betulae Pendulae extract is prepared by washing cortex Betulae Pendulae, drying, pulverizing, extracting with ethanol, concentrating, spray drying, and making into powder. Has antibacterial, antiviral, antitumor, blood lipid reducing, gallbladder function promoting, and liver protecting effects.

The cortex Magnolia officinalis extract is prepared by washing cortex Magnolia officinalis, drying, pulverizing, extracting with ethanol, concentrating, and spray drying to obtain cortex Magnolia officinalis extract powder. Has antibacterial and dental caries preventing effects.

As a preferred technical scheme of the invention, the composition consists of the following components in parts:

furthermore, the invention also discloses that the effect of the composition can be further improved by adding one or more of honeysuckle extract, isatis root extract, dyers woad leaf extract, dandelion extract, fructus forsythiae extract, calculus bovis factitius, azulene sodium sulfonate, scutellaria extract, coptis extract, andrographis extract, glycyrrhetinic acid, wormwood extract, mulberry leaf extract, honeysuckle flower extract and watermelon crystal, and the dosage is as follows:

the honeysuckle extract is obtained by water extraction and alcohol precipitation of honeysuckle, has wide pharmacological effects, and mainly comprises anti-inflammatory, antipyretic, antibacterial, antiviral, antifungal, antiendotoxin, antihypertensive, liver-protecting and gallbladder-benefiting, antifertility, antioxidation, hemostasis, immunoregulation and the like. The honeysuckle plays roles of clearing heat and reducing internal heat, diminishing inflammation and relieving pain and removing halitosis in the toothpaste.

The radix Isatidis extract is alcoholic extract of radix Isatidis, and has antibacterial, antiinflammatory, and antiviral effects.

The folium Isatidis extract is ethanol extract of folium Isatidis, and has antibacterial, antiinflammatory, and antiviral effects.

The herba Taraxaci extract is obtained by extracting herba Taraxaci with water and precipitating with ethanol. The herba Taraxaci is broad-spectrum antibacterial agent, and has antifungal, antiviral, antiinflammatory, antioxidant, antitumor, liver protecting, gallbladder promoting, gastrointestinal and blood sugar protecting, blood vessel function improving, immunity regulating, and antifatigue effects. Can inhibit the growth of oral Hp, treat recurrent oral ulcer, and eliminate halitosis in oral cavity; can be administered for treating gastric and duodenal ulcer. The herba Taraxaci extract has antibacterial and anti-inflammatory effects.

The fructus forsythiae extract is obtained by extracting fructus forsythiae with water and precipitating with ethanol, and has antibacterial and antiinflammatory effects.

The artificial bezoar has sweet and cool nature and flavor, enters heart and liver meridians, has the effects of clearing away heat and toxic materials, reducing phlegm and arresting convulsion, and is used for treating phlegm heat delirium, coma and aphasia, acute infantile convulsion, sore throat, aphtha in mouth and tongue, pain and swelling and sore. Has antibacterial and antiinflammatory effects.

The sodium azulene sulfonate (the systematic name is 1, 4-dimethyl-7-isopropyl azulene-3-sodium sulfonate) is prepared by modifying the molecular structure of guaiazulene.

The Scutellariae radix extract is prepared by washing Scutellariae radix, drying, pulverizing, extracting with ethanol, concentrating, spray drying, and making into Scutellariae radix extract powder. Baicalin as herbal additive of toothpaste has hemostatic, antiinflammatory, antibacterial, halitosis removing and wound healing effects.

The Coptidis rhizoma extract is an alcoholic extract of Coptidis rhizoma, which is prepared by washing Coptidis rhizoma, drying, pulverizing, extracting with ethanol, concentrating, spray drying, and making into Coptidis rhizoma extract powder. The Coptidis rhizoma has antibacterial, antiinflammatory, antiviral, endotoxin and antiprotozoal effects, and can be used for inhibiting. Berberine in Coptidis rhizoma has inhibitory effect on various gram-positive and gram-negative bacteria. The berberine has obvious inhibition effect on helicobacter pylori in vitro, and has low price and good safety.

The herba Andrographitis extract is alcoholic extract of herba Andrographitis, and is prepared by washing, drying, pulverizing, extracting with ethanol, concentrating, spray drying, and making into powder. Can be used for treating common cold, fever, sore throat, aphtha of the mouth and tongue, cough due to cough, etc. The andrographis paniculata extract mainly contains lactone and flavonoid, the effective component mainly contains lactone, and the andrographis paniculata extract has good heat-clearing and anti-inflammation effects and is a good reputation of traditional Chinese medicine antibiotics. Modern pharmacological studies show that andrographolide has antiinflammatory, antitumor, antiviral, antioxidant, immunoregulatory and hepatoprotective effects. Experiments prove that the andrographis paniculata extract can treat oral ulcer.

Glycyrrhetinic acid has antibacterial, antiallergic, skin whitening, antiviral, and antiinflammatory effects.

The folium Artemisiae Argyi extract is ethanol extract of folium Artemisiae Argyi, and has antibacterial, antiinflammatory, antiasthmatic, antitussive, and antiallergic effects.

The mulberry leaf extract is an aqueous extract of mulberry leaves, has the effects of enhancing immunity, diminishing inflammation, inhibiting bacteria, resisting viruses, resisting coagulation, expanding blood vessels and the like, and has a strong effect of inhibiting common pathogenic bacteria in the oral cavity.

The flos Lonicerae extract is water extract of Lonicera Japonica, and has antibacterial and antiinflammatory effects.

The watermelon crystal has antibacterial, antiinflammatory, antipyretic, and analgesic effects, and can be used for treating oral ulcer.

As a preferred embodiment of the present invention, the composition consists of the following components:

further, in the composition, when the total content (by mass) of the sea salt and the isatis root exceeds 18% of the composition, the total content of the fructus forsythiae extract, the azulene sodium sulfonate, the coptis extract, the andrographis paniculata extract and the watermelon crystal is required to be lower than 7%;

more preferably, the composition consists of:

or the like, or, alternatively,

in the composition, when the total content of fructus forsythiae extract, azulene sodium sulfonate, coptis extract, andrographis paniculata extract and watermelon crystal is higher than 22%, the total content of sea salt and isatis root is lower than 17%;

more preferably, the composition consists of:

the composition provided by the invention has the advantages that specific components are selected, different medicinal mechanisms of different components are utilized, specific proportions are combined, and the synergistic effect among the components is fully utilized, so that the obtained composition has the effects of efficiently inhibiting bacteria, eliminating helicobacter pylori, relieving gingival inflammation and removing halitosis. Meanwhile, the composition provided by the invention has better effects of inhibiting bacteria, eliminating helicobacter pylori, relieving gingival inflammation and removing halitosis, is reasonable in cost, simple and convenient to prepare, safe to use and strong in universality, and is more suitable for commercial popularization.

The invention also provides application of the composition in any one of the technical schemes in preparing a product for treating bacteriostasis, eliminating helicobacter pylori, relieving gingival inflammation, removing halitosis or oral care.

The invention provides toothpaste which comprises the following components in parts by weight:

active components:

auxiliary materials:

the toothpaste provided by the above method can maximally utilize the synergistic effect of each component through the special selection of the components and the regulation and control of the components, has better treatment or adjuvant treatment effect on oral diseases, and particularly has higher treatment effect on gingival bleeding symptoms.

Preferably, the toothpaste comprises the following components in parts by weight:

active components:

auxiliary materials:

the abrasive is selected from one or more of silicon dioxide, hydrated calcium hydrophosphate, anhydrous calcium hydrophosphate, calcium carbonate, aluminum hydroxide, sodium chloride or calcium pyrophosphate;

the humectant is selected from one or more of sorbitol, glycerol, propylene glycol, polyethylene glycol-8 or butanediol;

the foaming agent is selected from one or more of sodium dodecyl sulfate, sodium lauroyl sarcosinate, sodium cocoyl glycinate, sodium acyl glutamate or sodium cocoyl methyl taurate;

the thickening agent is selected from one or more of cellulose and derivatives thereof, guar gum, sodium alginate, xanthan gum, guar gum hydroxypropyl trimethyl ammonium chloride, carrageenan or locust bean;

the sweetener is selected from one or more of saccharin sodium, sucralose, xylitol, sodium cyclamate, aspartame, stevioside or mogroside;

the essence is selected from one or more of vanilla essence, spearmint essence, mint essence or wintergreen mint essence;

the preservative is selected from one or more of benzoic acid and salts thereof, sorbic acid and salts thereof or parabens;

the appearance modifier is selected from one or more of titanium dioxide, food pigment, food color lake or tooth cleaning particle;

the solvent is selected from deionized water.

Preferably, the active component of the toothpaste further comprises at least one of the following ingredients:

as the most preferred embodiment of the toothpaste of the present invention, the toothpaste comprises the active ingredient together with the following adjuvants:

the invention also provides a preparation method of the toothpaste, which comprises the following steps: mixing the above active ingredients, and making toothpaste. Preferably, the toothpaste is prepared by mixing all the above components.

The preparation method of the toothpaste comprises the following steps:

A. dissolving the active component in a humectant, and stirring at high speed to prepare a first mixed solution;

B. adding a thickening agent into the first mixed solution, and stirring to obtain a second mixed solution;

C. adding an appearance modifier into the second mixed solution, and stirring to obtain a third mixed solution;

D. adding the third mixed solution, the sweetening agent, the friction agent, the essence, the foaming agent and the preservative into the solvent, and stirring to obtain a fourth mixed solution;

E. the fourth mixed solution is continuously stirred under the vacuum condition of 0.08-0.12MPa to prepare paste.

F. Checking, aseptically filling into aluminum plastic composite tube, sealing, and making into toothpaste.

The invention also provides mouthwash, which comprises the following components in parts by weight:

active components:

auxiliary materials:

the solvent is selected from deionized water;

the humectant is selected from one or more of sorbitol, propylene glycol, glycerol and polyethylene glycol-8;

the surfactant is selected from one or more of poloxamer 407, sodium lauryl sulfate, sodium lauroyl sarcosinate and sodium cocoyl methyl taurate;

the thickening agent is selected from one or more of cellulose, carbomer and carrageenan;

the edible essence is selected from one or more of flos Caryophylli, cortex Cinnamomi, flos Osmanthi Fragrantis, oleum Menthae Dementholatum, herb Hierochloes Adoratae essence, herba Menthae Rotundifoliae, herba Menthae Haplocalycis, and herba Menthae Dementholati;

the colorant is selected from one or more of green, blue and red.

More preferably, the mouthwash further comprises the following components:

the technical scheme of the invention has the following beneficial effects:

the invention utilizes different medicinal mechanisms of each component, fully exerts the synergistic interaction effect of each component, and ensures that the obtained composition can prevent and assist in treating gingivitis (gingival bleeding) and simultaneously can inhibit the formation of dental plaque.

Detailed Description

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

Example 1

This example provides a composition formulated as follows:

example 2

This example provides a composition formulated as follows:

example 3

This example provides a composition formulated as follows:

example 4

This example provides a composition formulated as follows:

example 5

This example provides a composition formulated as follows:

example 6

This example provides a composition formulated as follows:

example 7

This example provides a composition formulated as follows:

example 8

This example provides a composition formulated as follows:

example 9

The embodiment provides a toothpaste, the total weight of which is 100 parts, and the formula of the toothpaste is as follows:

example 10

example 11

example 12

example 13

example 14

example 15

example 16

example 17

The embodiment provides mouthwash, the total weight of which is 100 parts, and the formula of which is as follows:

example 18

example 19

example 20

example 21

This example provides a method of making the toothpaste provided in examples 9-16, comprising the steps of:

(it will be understood by those skilled in the art that when a toothpaste does not contain a component, the component is not added during the preparation process)

A. Dissolving fel Ursi extract, herba seu radix Cirsii Japonici extract, herba Cephalanoploris extract, radix Sangusorbae extract, rhizoma Bletillae extract, Notoginseng radix extract, pollen Typhae extract, folium Artemisiae Argyi extract, flos Sophorae Immaturus extract, and ramulus Uncariae cum uncis extract in sorbitol and polyethylene glycol-8, stirring at high speed for 10min to obtain first mixture;

B. adding xanthan gum into the first mixed solution, and stirring for 10min to obtain a second mixed solution;

C. adding titanium dioxide into the second mixed solution, and stirring for 10min to obtain a third mixed solution;

D. adding water, saccharin sodium, xylitol, silicon dioxide, Mentha spicata, sodium cocoyl methyl taurate, and potassium sorbate into the third mixture, and stirring for 10min to obtain a fourth mixture;

E. continuously stirring the fourth mixed solution for 15min under the vacuum condition of 0.08-0.12MPa to prepare paste;

Comparative examples 1 to 3

Comparative examples 1-3 each provide a composition having the formula shown in the following table:

comparative examples 4 to 7

Comparative examples 4-7 each provide a composition having the formula shown in the following table:

comparative examples 8 to 10

Comparative examples 8-10 each provide a toothpaste having a total weight of 100 parts and a formulation as shown in the following table:

comparative examples 11 to 14

Comparative examples 11-14 each provide a toothpaste having a total weight of 100 parts and a formulation as shown in the following table:

comparative example 15

This comparative example provides an approved patented toothpaste formulation that has the efficacy of eliminating helicobacter pylori. The functional components comprise the following components in percentage by weight:

test example 1

This test example provides bacteriostatic tests of the compositions provided in examples 1-4 and comparative examples 1-3.

Test subjects: the compositions provided in examples 1 to 4 and comparative examples 1 to 3 were each prepared in the same parts by mass to sterile solutions. (the preparation method is carried out according to the conventional operation in the field)

The test method comprises the following steps:

the following strains causing oral diseases are selected: staphylococcus aureus, candida albicans, streptococcus mutans, streptococcus; selecting hydrolyzed casein (MH) broth and agar culture medium. After the standard strains are subjected to passage pure culture, a ring of strains are inoculated in MH broth culture medium in a constant temperature incubator at 37 ℃ for 6 hours by using an inoculating ring, and the bacterial quantity is identified and counted by a turbidimetric method. Taking the same amount of test bacterial liquid cultured for 6 hours, respectively and uniformly coating the bacterial liquid on the surface of an MH agar plate by using a sterile cotton swab, respectively absorbing the same amount of circular filter paper sheets of the sterile solutions of comparative examples 1-3 and examples 1-4 by using sterile tweezers after the bacterial liquid is slightly dried, attaching the circular filter paper sheets on the surface of the MH agar plate, culturing the circular filter paper sheets for 24 hours at 37 ℃ in an incubator, observing and measuring the diameter (mm) of a bacteriostatic ring, repeating the bacteriostatic experiment for 3 times, wherein the bacteriostatic result is the average value of 3 experiments.

And (3) test results: observing and measuring the diameter (mm) of a bacteriostatic circle, repeating bacteriostatic experiments for 3 times, wherein the bacteriostatic result is the average value of the 3 experiments to obtain the result shown in the following table 1;

the MH broth medium containing the same amount of the sterile solutions of comparative examples 1 to 5 and examples 1 to 4 was inoculated with 0.05ml (10 ml) of the test bacterial liquid cultured for 6 hours in the same amount as described above⁵CFU/ml) and inoculated with the same species as a control in sterile MH broth medium, incubated at 37 ℃ for 24 hours in an incubator with bacterial growth as shown in table 2;

TABLE 1

TABLE 2

Note: "+ + + +" bacteria clearly grew; "+" bacteria reduced growth; "0" is the sterile growth in culture.

Test example 2

This test example provides bacteriostatic tests of the compositions provided in examples 5-8 and comparative examples 4-7.

Test subjects: the compositions provided in examples 5 to 8 and comparative examples 4 to 7 were each prepared in the same parts by mass to sterile solutions. (the preparation method is carried out according to the conventional operation in the field)

The test method comprises the following steps:

the following strains causing oral diseases are selected: staphylococcus aureus, candida albicans, streptococcus mutans, streptococcus; selecting hydrolyzed casein (MH) broth and agar culture medium. After the standard strains are subjected to passage pure culture, a ring of strains are inoculated in MH broth culture medium in a constant temperature incubator at 37 ℃ for 6 hours by using an inoculating ring, and the bacterial quantity is identified and counted by a turbidimetric method. Taking the same amount of test bacterial liquid cultured for 6 hours, respectively and uniformly coating the bacterial liquid on the surface of an MH agar plate by using a sterile cotton swab, respectively absorbing the same amount of circular filter paper sheets of the sterile solutions of comparative examples 6-9 and examples 5-8 by using sterile tweezers after the bacterial liquid is slightly dried, respectively attaching the circular filter paper sheets on the surface of the MH agar plate, culturing the circular filter paper sheets in an incubator at 37 ℃ for 24 hours, observing and measuring the diameter (mm) of a bacteriostatic ring, repeating bacteriostatic experiments for 3 times, wherein the bacteriostatic result is the average value of 3 experiments.

And (3) test results: observing and measuring the diameter (mm) of a bacteriostatic circle, repeating bacteriostatic experiments for 3 times, wherein the bacteriostatic result is the average value of the 3 experiments to obtain the result shown in the following table 3;

the MH broth medium containing the same amount of the sterile solutions of examples 5 to 8 and comparative examples 4 to 7 was inoculated with 0.05ml (10) of the same amount of test bacterial liquid cultured in (1) for 6 hours⁵CFU/ml) and inoculated with the same species as a control in sterile MH broth medium, incubated at 37 ℃ for 24 hours in an incubator, the bacterial growth being as shown in table 4;

TABLE 3

TABLE 4

Test example 3

The present test example provides the human test (treatment effect on halitosis) of the toothpastes provided in examples 9 to 12 and comparative examples 8 to 10, and data thereof.

Volunteers with severe pathological halitosis (volunteers with various halitosis types) were selected to participate in the test of the treatment effect of toothpaste on halitosis. Before trial, the volunteers are scored and randomly grouped into 25 persons in each group, different toothpastes are tried in each group respectively, the toothpastes are scored once in the morning and at night every day, and the halitosis scoring is carried out again after 30 days. Halitosis scoring the scoring protocol of Rosenberg was used, as follows:

0 point, no halitosis, and definitely no odor.

Score 1, suspected bad breath, similar to an audible smell.

2 points, slight halitosis, definitely smells bad smell, but very slight.

Score 3, moderate halitosis, obvious halitosis.

4 points, severe halitosis, but it is barely tolerated by the examiners.

5 points out strong odor, which is intolerable to the examiners.

The results are shown in Table 5:

TABLE 5 therapeutic effect on pathological halitosis using different toothpastes

Test example 4

The present test example provides the human tests (treatment effect on halitosis) of the toothpastes provided in examples 13 to 16 and comparative examples 11 to 14, and data thereof.

0 point, no halitosis, and definitely no odor.

Score 1, suspected bad breath, similar to an audible smell.

2 points, slight halitosis, definitely smells bad smell, but very slight.

Score 3, moderate halitosis, obvious halitosis.

4 points, severe halitosis, but it is barely tolerated by the examiners.

5 points out strong odor, which is intolerable to the examiners.

The results are shown in Table 6:

TABLE 6 therapeutic effect on pathological halitosis using different toothpastes

Test example 5

Human body test (for gingivitis treatment)

Volunteers with symptoms of gingival swelling and bleeding are selected to participate in the human body use test of the toothpaste, the test samples are randomly divided into 25 persons in each group, the toothpaste in the embodiment and the comparative example is used for each group, the group is used once in the morning and at night every day, and the gingivitis treatment effect is investigated after four weeks.

The criteria for therapeutic effect are as follows:

and (3) curing: the subjective symptoms of the patient disappear, the gingival inflammation disappears completely, the damaged gingival connective tissue can see new collagen fibers, and the function, color, shape and quality of the gingiva are all recovered to be normal;

improvement: the patient's gingiva and gingival papilla are reddish, have slight edema, hyperemia, and are brittle, and have little punctate bleeding when the gingival sulcus is touched by a blunt round probe;

and (4) invalidation: the patient's gingiva still had swelling and pain, and bleeding was seen in probing without improvement of symptoms and signs.

The total effective rate of treatment is (cure + improvement)/total number of cases is multiplied by 100%.

The results are shown in Table 7:

TABLE 7

Test example 6

Human body test (for gingivitis treatment)

The criteria for therapeutic effect are as follows:

The results are shown in Table 8:

TABLE 8

Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. The toothpaste is characterized by comprising the following active ingredients:

2. the toothpaste according to claim 1, further comprising:

the solvent is selected from deionized water.

3. A method of preparing a toothpaste according to claim 1 or 2 comprising the steps of: