KR101985771B1 - Foaming solid formulation for mouthwash and preperation method thereof - Google Patents

Abstract

The present invention relates to a foaming solid formulation for mouthwash. More specifically, the present invention relates to a foaming solid formulation for mouthwash, comprising a mixture of grape extract, green tea extract, Scutellaria baicalensis extract and bellflower extract as an active ingredient; being excellent in dentin remineralization and dentinal tubule closing effect; not requiring brushing since the foaming solid formulation melts while generating bubbles when put in the mouth; being excellent in cleaning effect; and having anti-inflammatory, antibacterial, anti-cavity, odor elimination and scaling effects.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to foamable solid formulations for mouthwashes,

The present invention relates to a foamable solid agent for oral cleaning, and more particularly to a foamable solid agent for oral cleaning comprising a mixture of grape extract, green tea extract, golden extract and platycodone extract as an active ingredient.

Toothpaste is also referred to as dentifrice. It is a detergent used in combination with a toothbrush to clean the surface of teeth in the mouth during toothbrushing, which corresponds to a quasi-drug. The basic purpose of using toothpaste is to effectively remove the bacterial plaque attached to the tooth surface. In addition, it has been expanded for additional purposes such as dental caries, prevention of periodontal disease, whitening effect, slowing of hypersensitivity, and restraint of bad breath.

The main ingredients of toothpaste are abrasive, water, moisturizer, detergent, binder, flavor, sweetener, prophylactic agent, pigment and preservative. An effective ingredient such as a drug or a chemical substance having a dental caries prevention function and a periodontal disease prevention function is selectively added by the effect.

Generally, toothpastes are made of paste, powder, gel, mucilage and liquid phase. Among them, most of the pastes are circulated, but there is a high possibility of damaging the tooth enamel layer by the abrasive ingredient. There is a problem that toothpaste remains. In addition, the powder phase is disadvantageous in that the particles are sprayed or scattered during use, which is inconvenient for use, and the gel phase has low spreadability due to high viscosity and is not easily dispersed in the oral cavity. In addition, the liquid phase flows easily due to the low viscosity, and it is difficult to expect a sufficient cleaning effect due to the lack of cleaning components.

Accordingly, various formulations of various formulations have been developed for a specific use and a proper prescription for a subject, and techniques for reusing various ingredients commonly used are being developed. For example, Korean Patent Laid-Open Publication No. 10-2012-0079537 discloses a sheet-type toothpaste, Korean Patent Laid-open Publication No. 10-2015-0051296 discloses a foamed toothpaste in the form of beads, Korean Patent Publication No. 10-2004-0008953 A toothpaste in the form of a pill is disclosed. However, although the developed toothpaste can excite the curiosity of the consumer by going beyond the usual formulation of the detergent, the conventional toothpaste has a drawback in that it is merely deformed or deteriorated in cleaning effect, and still requires brushing . Therefore, it is required to develop a sterilizing agent which is easy to use and has improved efficacy.

Resverrol, known as a harmless and safe natural extract, is a kind of polyphenol contained in peanut and grape extract plants. When plants are exposed to external stresses such as ultraviolet rays and pathogens, It is one of the phytoalexin compounds (bio-defense substances). Also known as green tea extract, catechin belongs to the flavonoid group flavan-3-ols and is a kind of polyphenol. It is known to have carcinogenesis inhibition, arteriosclerosis, antiviral, anti-obesity, anti-diabetic, antibacterial, detoxifying and anti-inflammatory effects, and especially prevention of dental caries and prevention of bad breath. Baikalin is one of the flavonoids contained in Lamiaceae and Roots, and has been used in various forms due to its anti-inflammatory and antioxidant properties.

Accordingly, in the present invention, a foamable solid agent for oral cleaning, which contains only natural substances as an active ingredient, has been developed without excluding harmful synthetic chemicals.

KR 10-1448283 B1 KR 10-2005-0086796 A KR 10-1056573 B1

In order to solve the above problems, the present invention has been made to solve the above-mentioned problems and it is an object of the present invention to provide a foamable oral composition for oral cleaning, which comprises a mixture of grape extract, green tea extract, golden extract and bellflower extract as an active ingredient, The purpose is to provide brothers.

In order to achieve the above object,

A foamable solid for oral care cleansing, which comprises a mixture of grape extract, green tea extract, golden extract and bellflower extract as an active ingredient, is provided.

The grape extract contains resveratrol as an active ingredient, the green tea extract contains catechin as an active ingredient, the golden extract contains baicalin as an active ingredient, and the bellflower extract is gill.

The foamable solid agent for oral cleaning includes sodium hydrogen carbonate as a foaming agent and further comprises a surfactant, a pH adjuster, a formulation agent, an abrasive, a disintegrant, a lubricant, a sweetener and a perfume.

The grape extract, the green tea extract, the gold extract and the bellflower extract are in a weight ratio of 1: 7: 2: 4, and the foaming agent comprises 20 parts by weight per 100 parts by weight of the whole composition.

Wherein the pH regulator is DL-malic acid, and the surfactant is L-Lysin and an olive oil, wherein the surfactant is at least one selected from the group consisting of L- .

The foamable solid for oral cleaning is foamed within 30 seconds after the oral injection, and has dentin remineralization and ivory tubule closure ability.

In another aspect of the present invention, there is also provided a method for producing a pharmaceutical composition, which comprises mixing a mixture comprising a grape extract, a green tea extract, a golden extract and a bellflower extract, a surfactant, a pH adjusting agent, a preparation agent, an abrasive, a disintegrant, a lubricant, Mixing at a rate of 30 rpm or less under conditions of 40% or less for 15 minutes; Aging the mixture for 8 hours in a sealed state at a temperature of 25 DEG C or less and a humidity of 60% or less; Subjecting the aged mixture to a pressure of 2,000 kg / cm 2 at a speed of 30 rpm at a temperature of 20 ° C or less and a humidity of 40% or less; Aging the compressed mixture for 8 hours in a sealed state at a temperature of 25 DEG C or less and a humidity of 60% or less; The aged mixture was first coated at 1.5 rpm for 90 minutes and 2.5 rpm for 60 minutes under conditions of a heat exchanger pressure of 0.2 Mpsi, an air compressor pressure of 4 to 6 bar, and a gun injection pressure of 5 bar, followed by 60 minutes at 3.5 rpm, second coating for 30 minutes at rpm; Drying the coated mixture for 8 hours in a sealed state under the conditions of a temperature of 25 DEG C or less and a humidity of 60% or less. The present invention also provides a method for producing the foamable solid material for oral cleaning.

The foamable coagulant for oral cleaning according to the present invention is a new solid type and can be easily carried, easily and easily administered without water or toothbrush when going out without being at home or at home. In addition, the abundant bubbles penetrate quickly, and it is possible to clean all corners, and the tooth protecting and cleaning effect is faster than the conventional technique, and the teeth are not damaged by protecting the gums due to the size-specific abrasive. This is effective for people with weak teeth and teeth and gums, and after tooth brushing, there is no residue left. Also, it has excellent dentin remineralization and dentinal tubule closing effect, has anti-inflammatory and anti-bacterial function, has cavitation prevention, bad breath removal, and scaling effect.

FIG. 1 is a graph showing changes in gingival index of the nipple margins.
Figure 2 is a graph of Talbott's gingival index change.
FIG. 3 is a graph showing changes in the dental surface bacteria ability management ability index.
FIG. 4 is a graph showing a change in the tooth surface of the tooth surface of the tooth surface.
5 is a graph showing a change in the breath change measured with a breath analyzer.
6 is a graph of sensory evaluation change.
Fig. 7 is a SEM image of a remineralization test of dental caries. Fig.
8 is a SEM image of an artificial iv tubule closure effect test.
FIG. 9 is a graph showing the effect of removing the bad breath according to the hydrogen sulfide concentration.
10 is a graph showing a test for the effect of removing malodor according to methylmercaptan concentration.

Hereinafter, the foamable solid for oral care of the present invention will be described in more detail.

The extract of the present invention contains resveratrol, the green tea extract is catechin, and the gold extract is selected from the group consisting of Baikal It is preferable that lean extract is contained as an active ingredient, and the above-mentioned bellflower extract is of a long diameter.

The resveratrol is a polyphenolic substance found in more than 70 kinds of plants such as peanut, grape, pine, red wine and the like, and exists in two forms as cis-resveratrol and trans-resveratrol as shown in the following structure. Resveratrol has been shown to act as phytoalexin, an antibiotic substance that plants produce to fight bacteria and fungi when they are infected in the 1970s, and anticancer effects of rasberatrol in 1997 were reported In 2003, it was reported that yeast activates the longevity gene SIRT1. In addition, Lewis Doyle of the University of London, UK, has shown that resveratrol is effective in the treatment of asthma, chronic obstructive pulmonary disease (COPD) and arthritis due to its broad spectrum of inflammation-inhibiting effects. According to the report, resveratrol has been shown to provide several tens or hundreds of times better antioxidant activity than vitamins and minerals, as well as help prevent and treat gum disease by a team at the University of Quebec City, Laval, Party Achanandard. As described above, resveratrol is effective for the prevention and treatment of oral diseases by reporting the physiological activity effects such as anti-inflammation, antioxidation and prevention of gum disease.

The catechin is a type of polyphenol contained in green tea. EGCG (epigallocatechin gallate), EGC (epigallocatechin), ECG (epicatechin gallate) and EC (epicatechin) EGCG, which accounts for 40% of total polyphenols, is known to be the most physiologically active ingredient. The antioxidative and antimicrobial activities of catechins extracted from tea were reported (see, for example, Jang et al., 2004), and it has been reported that catechin has excellent antiseptic effect against oral bacteria (Korean Journal of Microbiology, Vol. 40, No. 4, 2004: 364-366) Horticulture Environment and Biotechnology, Vol.10, 2007: 114-114). In addition, it has been reported that it has various physiological activities and pharmacological effects such as anticancer, bad breath removal, tooth discoloration prevention, and the like, by inhibiting the activity of the substance causing the tartar formation and by preventing the alveolar bone loss and the healing of damaged gums through the antioxidative effect. The structure of catechin is shown below.

The baicalin is a type of flavonoid contained in the roots of Lamiaceae and Golden (Scutellaria baicalensis Georgi), and its major physiological activity is antimicrobial and anti-inflammatory activity. In addition, it has antiviral, antioxidant and anti-cancer effects . For example, it has been reported that gold extracts have antimicrobial activity and adhesion inhibitory effect against Streptococcus mutans, a causative agent of dental caries (Journal of Dental Hygiene Science, Vol 8, No. 4, 2008: 367-373) The physiological activity of lean can be utilized for the prevention of periodontal diseases and oral care.

The Platycodonis Radix is an excellent alkaline food having a wide variety of active ingredients such as protein, carbohydrate, lipid, minerals, vitamins and saponin as well as abundant fiber, iron, and calcium. Gilgye contains triterpenoid saponin and phytosterol components, and triterpenoid saponin has an anti-inflammatory effect. It is used in the lungs to treat symptoms that have many seawater and sputum and uncomfortable breathing. It is used for sore throat, cold cough, sputum, nasal obstruction, asthma, bronchial inflammation, pleurisy, headache, chills and tonsillitis. In addition, pharmacological actions have been reported, such as gadam action, hypoglycemic action, cholesterol lowering action,

The grape extract, the green tea extract, the gold extract, and the bellflower extract preferably comprise 1: 7: 2: 4 by weight.

It is preferable that the foamable solid for oral cleaning contains sodium bicarbonate as a foaming agent for foaming, and the foaming agent preferably includes 20 parts by weight for 100 parts by weight of the total composition. The sodium bicarbonate has a soft polishing power, has an excellent inhibitory effect on bacteria in the oral cavity, and neutralizes the organic acid generated by the plaque, thereby preventing tooth decay.

Further, the solid agent preferably further comprises a surfactant, a pH adjusting agent, a formulating agent, an abrasive, a disintegrating agent, a lubricant, a sweetening agent and a perfume.

Preferably, the surfactant is L-Lysin and olive oil, and the pH regulator is DL-malic acid.

It is preferable that the formulation agent includes crystalline cellulose, which is added for the purpose of binding a foaming agent, an abrasive and the like, and for producing a solid.

The abrasive agent preferably contains calcium carbonate and plays an important role in cleansing, such as removal of plaque and food. It should not damage the enamel of the teeth, and should be compatible with other components.

The disintegrant preferably comprises lactose and starch.

The lubricant is added to prevent impurities from being trapped in the punches of the tablet machine during the tableting process, and preferably includes magnesium stearate.

The sweetener is added to overcome the basic taste of the solid agent and may be L-menthol, D-sorbitol, xylitol or enzyme-treated stevia.

The fragrance is added to enhance, deform or inhibit the fragrance.

The solid agent is foamed within 30 seconds after oral administration, and has dentin remineralization and ivory tubule closure ability.

In another aspect of the present invention, there is also provided a method for producing a pharmaceutical composition, which comprises mixing a mixture comprising a grape extract, a green tea extract, a golden extract and a bellflower extract, a surfactant, a pH adjusting agent, a preparation agent, an abrasive, a disintegrant, a lubricant, Mixing at a rate of 30 rpm or less under conditions of 40% or less for 15 minutes; Aging the mixture for 8 hours in a sealed state at a temperature of 25 DEG C or less and a humidity of 60% or less; Subjecting the aged mixture to a pressure of 2,000 kg / cm 2 at a speed of 30 rpm at a temperature of 20 ° C or less and a humidity of 40% or less; Aging the compressed mixture for 8 hours in a sealed state at a temperature of 25 DEG C or less and a humidity of 60% or less; The aged mixture was first coated at 1.5 rpm for 90 minutes and 2.5 rpm for 60 minutes under conditions of a heat exchanger pressure of 0.2 Mpsi, an air compressor pressure of 4 to 6 bar, and a gun injection pressure of 5 bar, followed by 60 minutes at 3.5 rpm, second coating for 30 minutes at rpm; Drying the coated mixture for 8 hours in a sealed state under the conditions of a temperature of 25 DEG C or less and a humidity of 60% or less. The present invention also provides a method for producing the foamable solid material for oral cleaning.

Hereinafter, the present invention will be described in more detail with reference to examples. However, the following examples are intended to illustrate the present invention, and the scope of the present invention is not limited thereto.

Example

The ingredients according to Table 1 were mixed using a ribbon mixer (V-Mixer) at a temperature of 22 ° C or less and a humidity of 40% or less at a rate of 30 rpm or less for 15 minutes, And aged for 8 hours in a sealed state under the condition of 60% or less. The aged raw materials were compressed at a pressure of 2,000 kg / cm 2 and a speed of 30 rpm using a tableting machine under the conditions of a temperature of 20 ° C or less and a humidity of 40% or less, and then sealed in a sealed state under the conditions of a temperature of 25 ° C or less and a humidity of 60% Lt; / RTI > Then, the aged raw materials were firstly coated using a coater under the conditions of a heat exchanger pressure of 0.2 Mpsi, a main air compressor pressure of 4 to 6 bar, and a gun injection pressure of 5 bar. The exhaust temperature was 35 to 38 ° C, 1.5 rpm for 90 minutes, 2.5 rpm for 60 minutes. Subsequently, the secondary coating was performed under the same conditions as the primary coating, and the exhaust temperature was 45 to 48 ° C., and the coating was carried out at 3.5 rpm for 60 minutes and 4.5 rpm for 30 minutes. Thereafter, it was dried in a sealed state for 8 hours at a temperature of 25 DEG C or less and a humidity of 60% or less.

Ingredient name (main ingredient name) Weight portion Weight (mg) main ingredient Green tea extract (catechin) 0.35 2.8 Grape Extract (Resveratrol) 0.05 0.4 Golden extract (Baikal) 0.1 0.8 Bellflower extract (Gakyung) 0.2 1.6 Sweetener Xylitol 27.48 219.84 L-menthol 0.8 6.4 D-sorbitol 14.1 112.8 Enzyme treatment Stevia 1.44 11.52 Surfactants Olive oil 3.3 26.4 L-Lysine 1.92 15.36 pH adjusting agent DL-malic acid 13.1 104.8 Additive for mold Magnesium stearate 0.48 3.84 Calcium carbonate 0.08 0.64 Sodium hydrogencarbonate 20 160 Crystalline cellulose 4.8 38.4 Lactose 6 48 Starch 5 40 Spices Mint flavor powder 0.8 6.4

Comparative Example 1 (Comparative Sediment)

Except that the main components were the same in the above Examples.

Comparative Example 2 (standard detergent)

Comparative Example 2 (standard detergent) comprising dental type silica as the main component and composed of only conventional detergent ingredients was prepared.

COMPARATIVE EXAMPLE 3 (Control Treatment)

A wetting agent, a foaming agent and an aroma were added to prepare a standard toothpaste solution of Comparative Example 3 (control toothpaste).

Selection of test subjects and test methods

120 men and women aged 20 years or older and 50 years old or younger who had mild-moderate gingivitis symptoms were selected as test subjects. The test subjects were divided into 30 test groups each after a 1-week run-in period , The control group, the negative control group, and the positive control group for 4 weeks. Tests 1 to 4 were performed at intervals of 1 week, 2 weeks, and 4 weeks.

In order to create an oral environment with the same toothpaste, all of the 4 groups were subjected to brushing using the rotation method using the Comparative Example 2 for 3 minutes after breakfast and after dinner. The test group was washed after lunch, the comparative group was compared with the control group 1 after lunch, and the positive control group was cleaned after the lunch group using the comparative example 3. The negative control group was further washed after breakfast and after dinner .

At this time, in the Example and Comparative Example 1, one tablet was poured into the mouth, and then the tooth was crushed and masticated. Then, the teeth, teeth and tongue were spit out by friction cleaning and goggle using the tongue and lips, . In Comparative Example 3, after the lunch, the dose (10 cc) was faded in the mouth for about 1 minute and used as a spit.

Table 2 below shows the sex and age distribution of all test subjects.

Total age group 20's 30s 40s Total sex 120 71 29 20 male 33 14 14 5 female 87 57 15 15

Test Example  One. Gingivitis index  evaluation

1) Papillary Marginal Attached Gingival Index (PMA index)

The gingival index of the marginal margin of the papilla was measured for the upper and lower anterior teeth. The results are shown in Table 3 and FIG. There was no statistically significant difference between the four groups (p <0.05). There was no statistically significant difference between the groups in the PMA index before the test (11.27 in the test group, 11.30 in the control group, 11.33 in the negative control group and 11.33 in the negative control group) , Negative control group 11.67, and positive control group 11.10. After 2 weeks, the PMA index was 10.93 in the test group, 10.80 in the control group, 11.63 in the negative control group and 10.80 in the positive control group. The PMA index was significantly lower in the test group and in the positive control group than in the negative control group (p <0.01) (p < 0.05), and the PMA index of the test group and the positive control group decreased to statistically significant level at 4 weeks after the test (p <0.05). From the above results, it was confirmed that the embodiment of the present invention shows gingival relaxation effect to the extent similar to the case of using a conventional mouthwash solution.

0 parking 1 parking 2 parking 4 parking Average Standard Deviation Average Standard Deviation Average Standard Deviation Average Standard Deviation Test group 11.27 2.95 11.03 2.89 10.93 2.42 10.20 2.30 Comparative group 11.30 2.59 11.07 2.86 10.80 2.01 11.07 2.21 Negative control group 11.33 2.88 11.67 2.95 11.63 2.39 11.90 2.26 Positive control group 11.30 2.63 11.10 2.81 10.80 1.95 10.20 2.27 p-value 1,000 0.805 0.401 0.011

2) Gingival Index (GI) of Talbott, Mandel and Chilton

e의 치은염 평점 기준에 따라서, 치아를 둘러싸고 있는 협설측 치은염을 각각 근심, 원심, 중앙 치은염으로 구분하여 6개 부위를 측정하고, 6개 부위의 측정값을 산술평균으로 구하였다. 개인의 치은염지수는 각 부위별 측정치의 합계를 검사대상치아수로 나누어 구하였으며, 이때 검사대상치아는 총 6개인 #16, #11, #26, #46, #31, #36번 치아를 대상으로 하였다. L

e의 부위별 치은염 평점 기준은 하기와 같으며, 결과값을 하기 표 4 및 도 2에 나타내었다.The gingival index was determined by Talbott, Mandel and Chilton

According to the gingival index of e, the gingivitis surrounding the teeth was divided into a mesial, a distal, and a central gingiva, respectively, and six areas were measured. The gingivitis index was calculated by dividing the gingival index by the total number of teeth measured for each site divided by the number of teeth to be examined. At this time, teeth of # 16, # 11, # 26, # 46, # 31, Respectively. L

The evaluation criteria of gingivitis according to the site of e are as follows, and the results are shown in Table 4 and FIG.

e의 부위별 치은염 평점 기준][L

e of gingivitis by site]

0: No inflammation

1: mild gingivitis (weak change in color tone and weak edema) - No bleeding in periodontal pouch

2: Middle gingivitis (redness, edema) - Hemorrhage in measuring periodontal pouch

3: High grade gingivitis (ulcer) - tendency to bleed spontaneously

There was no statistically significant difference in the Talbott's gingival index before the test between test group 1.33, 1.33, 1.36, 1.36, and 1.35, respectively. There was no significant difference between groups in test group 1.31, 1.35, 1.37, There was no significant difference between the groups. After 2 weeks, the measured values were 1.24 in the test group, 1.33 in the negative control group, 1.41 in the negative control group, 1.22 in the positive control group and 1.12 in the test group, 1.26 in the test group, 1.44 in the negative control group and 1.12 in the positive control group. And 4 weeks later, the gingival index of the test group and the positive control group was significantly lower than that of the test group (p <0.05). In the control group, the gingivitis index was significantly increased after 4 weeks (p <0.05). There was no statistically significant difference between the groups in terms of time before and 1 week after the test (p> 0.05), but the gingivitis index was significantly lower in the test group and the positive control group at 2 weeks than the control group and the negative control group p <0.05). The gingival index was significantly lower in the control group than in the negative control group (p <0.05). On the other hand, after 4 weeks, the gingival index of the positive control group was the lowest, but it was not significantly different from the test group, and it was found that there was a significant difference between the control group and the negative control group.

0 parking 1 parking 2 parking 4 parking Average Standard Deviation Average Standard Deviation Average Standard Deviation Average Standard Deviation Test group 1.33 0.19 1.31 0.16 1.24 0.13 1.19 0.13 Comparative germ 1.33 0.17 1.35 0.15 1.33 0.11 1.26 0.12 Negative control group 1.36 0.15 1.37 0.10 1.41 0.09 1.44 0.10 Positive control group 1.35 0.16 1.31 0.16 1.22 0.13 1.12 0.11 p-value 0.814 0.334 0.000 0.000

Test Example  2. Dentifrice membrane  evaluation

1) Patient Hygiene Performance Index (PHP index)

The number of teeth to be examined was # 16, # 11, # 26, # 46, # 31, # 36 teeth.

As shown in Table 5 and FIG. 3, the PHP index before the test was 2.23 in the test group, 2.16 in the comparative group, 2.16 in the negative control, 2.21 in the positive control group and the PHP index was 2.18 in the test group, 2.07 in the test group, In the control group 2.06, after 2 weeks, the PHP index was 2.07 in the test group, 2.15 in the comparative group, 2.21 in the negative control group, 2.09 in the positive control group and 2.09 in the positive control group, 2.09 in the test group, 2.09 in the test group, 2.09 in the negative control group and 2.08 in the negative control group. There was no significant difference between the 4 groups (p> 0.05), 4 weeks after the test, 1 week, and 2 weeks after the test, the PHP index was significantly lower in the test group than the negative control group (p <0.05) . From the above results, it was confirmed that the embodiment of the present invention is superior in the ability to inhibit the formation of dental plaque membrane as compared with the case without oral cleaning.

0 parking 1 parking 2 parking 4 parking Average Standard Deviation Average Standard Deviation Average Standard Deviation Average Standard Deviation Test group 2.23 0.39 2.18 0.36 2.07 0.32 2.04 0.37 Comparative group 2.16 0.49 2.07 0.45 2.15 0.35 2.09 0.43 Negative control group 2.16 0.37 2.21 0.30 2.21 0.36 2.35 0.35 Positive control group 2.21 0.41 2.06 0.44 2.09 0.34 2.08 0.41 p-value 0.833 0.376 0.424 0.011

2) Plaque index on the tooth surface

The degree of adhesion of the dental plaque was measured using Turesky 's modified Quigley and Hein tooth surface rating criteria. The teeth were # 15, # 13, # 26, # 44, # 32, # 36 teeth.

As shown in the following Table 6 and FIG. 4, Turesky's tooth surface bacterial counts were 2.58 in the test group, 2.60 in the test group, 2.55 in the negative control group, 2.58 in the positive control group, 2.53 in the test group, 2.53 in the test group, 2.56 in the negative control group, 2.51 in the positive control group, 2.55 in the test group, 2.47 in the control group, 2.57 in the negative control group, 2.49 in the positive control group, 2.44 in the test group in the fourth week, 2.47 in the comparison group, 2.67 in the negative control group and 2.45 in the positive control group. There was no significant difference between the groups (p> 0.05) before, 1 week, and 2 weeks after the test (p> 0.05). After 4 weeks, the test group and the positive control group showed significantly lower dental flora than the negative control group p &lt; 0.05).

0 parking 1 parking 2 parking 4 parking Average Standard Deviation Average Standard Deviation Average Standard Deviation Average Standard Deviation Test group 2.58 0.38 2.53 0.32 2.55 0.31 2.44 0.27 Comparative group 2.60 0.36 2.53 0.29 2.47 0.29 2.47 0.30 Negative control group 2.55 0.35 2.56 0.28 2.57 0.22 2.67 0.26 Positive control group 2.58 0.38 2.51 0.29 2.49 0.30 2.45 0.30 p-value 0.959 0.911 0.503 0.004

Test Example  3. Bad breath assessment

1) Bad breath measuring instrument

The bad breath evaluation was carried out using a BB Checker (Plustech, Daejeon, Korea). The measured values are shown in Table 7 and FIG.

The test results were as follows: test group 81.4, comparison group 82.3, negative control group 82.3, positive control group 81.3, test group 78.7, comparison group 81.5, negative control group 83.8, positive control group 77.4, test group 77.2, test group 82.2 , Negative control group (84.3) and positive control group (76.7). After 4 weeks, the test group was 73.6, the control group was 78.5, the negative control group was 87.3, and the positive control group was 72.8. There was no significant difference between the 4 groups (p> 0.05) before, 1 week, and 2 weeks after the test. After 4 weeks, the test group and the positive control group had lower levels of bad breath than the negative control group p < 0.05). The difference in time between the test group and the positive control group was significantly lower than that before the test (p <0.05). From the above results, it was confirmed that the embodiment of the present invention has a degree of halitosis reduction similar to that in the case of using a conventional mouthwash solution.

0 parking 1 parking 2 parking 4 parking Average Standard Deviation Average Standard Deviation Average Standard Deviation Average Standard Deviation Example 81.4 11.7 78.7 16.3 77.2 14.9 73.6 14.1 Comparative Example 1 82.3 12.9 81.5 12.7 82.2 10.8 78.5 13.0 Comparative Example 2 82.3 14.1 83.8 14.9 84.3 9.3 87.3 14.3 Comparative Example 3 81.3 12.4 77.4 12.4 76.7 11.5 72.8 13.3 p-value 0.984 0.309 0.034 0.000

2) Visual analogue scale test

Visual analogue scale was used to evaluate odor through sensory evaluation, which is shown in Table 8 and FIG. 6 below.

As a result, the degree of halitosis in the test group was lowered from 4.63 before the test to 4.27 after 4 weeks and was lower than that of the control group.

0 parking 1 parking 2 parking 4 parking Average Standard Deviation Average Standard Deviation Average Standard Deviation Average Standard Deviation Test group 4.63 0.67 4.47 0.78 4.53 0.73 4.27 0.83 Comparative group 4.67 0.66 4.57 0.57 4.50 0.68 4.30 0.79 Negative control group 4.60 0.67 4.50 0.63 4.73 0.78 4.60 0.56 Positive control group 4.53 0.68 4.60 0.50 4.37 0.67 4.37 0.76 p-value 0.885 0.838 0.266 0.305

Test Example  4. Stability evaluation

Adverse events following the use of the patch were identified and analyzed for stability. After two weeks and four weeks, the investigator was asked to confirm the presence of an adverse event, and follow-up on all adverse events found was allowed to proceed until the adverse event had disappeared, stabilized, or until the situation could be explained . The tester should be able to determine whether the relevance of the adverse reaction to the human application product is 'Possibly related', 'Probably related', 'Definitely related', 'Unknown' , The abnormal reaction was judged to be an abnormal reaction related to the product for human application test, and detailed information was provided separately. As a result of the evaluation, no adverse reaction was found in the test person during the entire test period.

Test Example  5. Dentin Artificial caries lesion Remineralization  evaluation

The dentin refers to a layer under enamel, which consists of 70% inorganic, 20% organic, and 10% water. When the dentin is exposed, it is not protected from external stimuli and the sensitivity of the teeth increases. As a result, all the stimuli from the outside are transmitted to the nerves in the dimension as they are, so that they are more sensitive to the same stimuli. It causes symptoms such as pain or sensory depression. Therefore, remineralization of demineralized dentin is very important for prevention of root caries and for the treatment of sensitive teeth.

The surface of the dentin artificial caries lesion was observed through a scanning electron microscope (SEM) after testing the example in an artificially created tooth lesion, and the result is shown in FIG. As a result of observation, the control group shown in FIG. 7 (a) showed irregular dentin surface morphology and collapsed appearance of demineralization. The dentin surface of the artificial caries lesion, We observed that the caries lesion disappeared and the surface of the missing tooth was re-calcified due to remineralization. In addition, as a result of measurement of dentin microhardness, the surface microhardness of the dentin increased to 140.26 HV (kg / mm ² ), indicating that calcification with a hard outer shape for dentin protection was achieved. Thus, it was confirmed that the embodiment of the present invention penetrates into the demineralized pores of the dentin to exert an excellent influence on the remineralization.

Test Example  6. Evaluation of closure effect and whitening effect on artificial iv tissue

Dental caries can be a bacterial infiltration route when dental caries are present or exposed, and when physical, chemical stimuli, and thermal stimuli are applied to the dentinal tubules in the exposed state, the liquid in the dentinal tubules moves in and out very quickly And the pressure caused by the movement of the iv liquid solution influences the cells in the dimension. As a result, the cells are stretched or compressed and the neurons in contact with the cells sense the change and feel the pain. Thereby closing the exposed dental tubules to protect teeth from gum disease and tooth decay, and to alleviate hardened caries and hypersensitivity.

In order to evaluate the effect of closing the iv tubule, the embodiment was tested on the artificial iv tissue, and the surface change was observed through a scanning electron microscope. The results are shown in FIG. As a result, the control group, Fig. 8 (a), was exposed to the dacryocystorhinostomy, whereas the treated artificial dacryocystillary tubule was deposited to the inside of the dacitic tubule and the diameter of the dacitic tubule was small, and 71.6% The results showed that the closure effect of dacitcial tubules was excellent. Also, it was confirmed that the measurement value was increased by 30.73 when the color was measured after the treatment of the example, and the whitening effect was observed.

Test Example  7. Assessment of bad breath removal

The cause of bad breath is mainly volatile sulfur compounds, and the main components are hydrogen sulfide (H ₂ S) and methyl mercaptan (CH ₃ SH). The concentration of hydrogen sulfide and methyl mercaptan in the oral cavity was measured to evaluate the effect of removing the bad breath. The bad breath was measured 4 times after the gas phase, immediately after the washing, 30 minutes after the washing and 1 hour after the washing. Comparative Example 1 was used as a control group and comparative analysis was performed using a commercially available gagrin as a comparative example .

As shown in FIG. 9, when the concentration of hydrogen sulfide was measured, the concentration of hydrogen sulfide was greatly reduced when the mouth was washed through the Examples and Comparative Examples as compared with the control group. In addition, the concentration immediately after use of the comparative example was 393 ppb, whereas the concentration immediately after the use of the example was 279 ppb, which indicates that the odor removal rate is better when the example is used. Even after 1 hour, the comparative example is 346 ppb In the comparative example, the value of 174 ppb is shown, which shows that the bad breath effect of the embodiment of the present invention is better.

In addition, as shown in Fig. 10, the concentration of methyl mercaptan was significantly lowered in Examples and Comparative Examples than in the control group. In addition, the concentration of methyl mercaptan immediately after use of the example was 112 ppb, whereas it was 169 ppb immediately after the use of the comparative example. Thus, it can be confirmed that the odor removal rate is better when the embodiment is used, and the concentration is low even after the lapse of time It can be confirmed that the bad breath removing effect is excellent.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. Accordingly, the embodiments disclosed herein are intended to be illustrative rather than limiting, and the spirit and scope of the present invention is not limited by these embodiments. The scope of protection of the present invention should be construed according to the following claims, and all the techniques within the scope of equivalents should be construed as falling within the scope of the present invention.

Claims (13)

In the foamable solid agent for oral cleaning,
Characterized in that the composition comprises 1: 7: 2: 4 by weight of a grape extract, a green tea extract, a golden extract and a bellflower extract as an active ingredient and has dentin remineralization and ivory tubule closure ability. brother
[Claim 2] The method according to claim 1, wherein the grape extract comprises resveratrol as an active ingredient, the green tea extract contains catechin as an active ingredient, and the gold extract comprises baicalin as an active ingredient. Solid brother
The oral care product according to claim 1, wherein the bellflower extract has a long diameter.
The foamable solid composition for oral cleaning according to claim 1, which comprises sodium hydrogencarbonate as a foaming agent
The foamable solid composition for oral cleaning according to claim 1, further comprising a surfactant, a pH adjusting agent, a formulating agent, an abrasive, a disintegrant, a lubricant, a sweetener and a flavoring agent
delete The foamable solid composition for oral cleaning according to claim 5, wherein the formulation agent is crystalline cellulose, the abrasive is calcium carbonate, the disintegrating agent is lactose and starch, and the lubricant is magnesium stearate.
The oral care cleansing composition according to claim 5, wherein the pH adjuster is DL-malic acid
The oral care cleansing composition according to claim 5, wherein the surfactant is L-Lysin and olive oil
The foamable solid composition for oral cleaning according to claim 1, which is foamed within 30 seconds after oral application
delete 5. The foamable solid composition for oral cleaning according to claim 4, wherein the foaming agent comprises 20 parts by weight per 100 parts by weight of the total composition
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