CN108606939B

CN108606939B - Composition with oral health care function and application and preparation method thereof

Info

Publication number: CN108606939B
Application number: CN201810210198.4A
Authority: CN
Inventors: 郭智华; 徐双华; 李宏杰
Original assignee: Holwray Pharmaceutical China Co ltd
Current assignee: Holwray Pharmaceutical China Co ltd
Priority date: 2018-03-14
Filing date: 2018-03-14
Publication date: 2020-07-07
Anticipated expiration: 2038-03-14
Also published as: CN108606939A

Abstract

The invention relates to a composition with an oral health function, and application and a preparation method thereof. The composition comprises, by weight, 0.01-2 parts of melaleuca alternifolia essential oil and 0.02-5 parts of arnica. The composition may further comprise one or more selected from sage, thyme leaf extract, folium Mori, flos Chrysanthemi, radix Puerariae, bupleuri radix, Scutellariae radix, Coptidis rhizoma, herba Andrographitis, flos Lonicerae, herba Taraxaci, and azulene sodium sulfonate. The invention utilizes different medicinal mechanisms of each component to fully play the synergistic interaction of the components, so that the obtained composition can prevent and assist in treating tooth sensitivity and inhibiting the formation of dental plaque, can inhibit a plurality of bacteria in oral cavities such as helicobacter pylori and the like, and has the effects of preventing tooth sensitivity, protecting gum and the like.

Description

Composition with oral health care function and application and preparation method thereof

Technical Field

The invention relates to the technical field of daily chemical products, in particular to a composition with an oral health function and an application and a preparation method thereof.

Background

Pathogenic bacteria in the oral cavity have certain harm to the health of the oral cavity. At present, research proves that the known bacterial species in the oral cavity can reach more than 700 species, and the oral cavity can keep a healthy state only by keeping good ecological balance among microorganisms in the oral cavity and between the microorganisms and host oral cavity tissues. If the proportion of individual or a plurality of microbes inherent in the oral cavity micro-ecology is obviously increased to become the advantage of a colonized part (especially the proportion of pathogenic bacteria) due to the influence of external microbes or other factors, the original oral cavity micro-ecology balance is broken, and flora imbalance can occur to cause oral diseases. Common oral diseases are: caries, periodontitis, gingivitis, halitosis, etc

When dental caries occurs in tooth enamel, the proportion of deformed streptococcus or marginal streptococcus in the flora on the enamel surface is obviously increased, and lactobacillus is closely related to the occurrence and the development of caries. Numerous studies have shown that Streptococcus mutans is a major cause of dental caries and it has been documented that Streptococcus mutans can also contribute to the development of gingivitis. Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans are anaerobic bacteria and play an important role in the onset of periodontitis. Candida albicans, Streptococcus, Staphylococcus aureus can cause bacterial stomatitis, Candida albicans can cause infections of the oral mucosa under certain conditions, and Staphylococcus aureus is closely associated with skin infections, tonsillitis and respiratory tract infections. Fusobacterium nucleatum, Treponema denticola, is the leading cause of halitosis.

In addition, in daily life, the teeth are often affected by cold, heat, acid and sweet to cause acid numbness and pain, which is commonly called tooth sensitivity. Most of the reasons are caused by dentin exposure due to tooth abrasion, gingival atrophy, tooth root exposure and the like, and sensitive symptoms are caused by conduction of dentin cytoplasm protrusions under re-stimulation; sensitization can also occur due to changes in the blood circulation of the pulp, such as dento-jaw trauma, and congestion of the apex of the root. With the age, the frequency of tooth sensitivity is more prominent, and the life quality of people is seriously influenced. Many toothpastes are commercially available to reduce or eliminate dentin-induced soreness by blocking dentinal tubules, inhibiting the flow of fluid within the tubules, and cutting off the conduction between dentin and pulp nerves. Strontium chloride and potassium nitrate can seal the tubules, thereby relieving the tooth sensitivity. But both strontium chloride and potassium nitrate have certain toxic and side effects.

Disclosure of Invention

In order to solve the above problems, the present invention provides an oral product which not only can clean the oral cavity, but also can inhibit various oral bacteria such as helicobacter pylori, etc., and has the effects of preventing tooth sensitivity, protecting gums, etc.

The invention is realized by adopting the following technical scheme. The composition for the oral cavity comprises the following components in parts by weight:

0.01-2 parts of melaleuca alternifolia essential oil;

0.02-5 parts by weight of arnica extract;

preferably, the first and second liquid crystal materials are,

0.05-1 part by weight of melaleuca alternifolia essential oil;

0.08-3 parts of arnica extract;

in the invention, the melaleuca alternifolia essential oil is an alcohol extract of melaleuca alternifolia. Melaleuca alternifolia (Melaleuca alternifolia), also known as tea tree, belongs to the genus Melaleuca of the family Myrtaceae, is native to the coastal zone of New southern Walsh, Australia, and is commonly used by folks to resist infection, diminish inflammation, and treat diarrhea and trauma. The cajeput essential oil extracted from fresh branches and leaves of cajeput is Tea tree oil (Tea tree oil), has broad-spectrum antibacterial, antifungal, antiviral and anti-inflammatory activities, and has strong antibacterial performance on escherichia coli, staphylococcus aureus, staphylococcus epidermidis, escherichia coli, pseudomonas aeruginosa, candida albicans and the like. In recent years, the method has been widely used for preparing skin external preparations, disinfectants, and perfumes and preservatives in cosmetics.

The arnica extract is tincture of arnica. Arnica montana (arnia montana) is a traditional medicine in europe and north america, and its head catkin has anti-inflammatory and wound healing effects. The arnica extract contains sesquiterpene lactone compounds and flavonoid compounds as main active components, and can be used for treating myalgia and rheumatalgia due to blood stasis sprain, and stimulating immune system. Use of arnica extract for treating skeletal muscle diseases, promoting healing after topical injury, reducing bruise and alleviating muscle pain; can be used for treating skin scar after operation, laser treatment or trauma. At present, Arnica herb tincture, ointment and gel are sold in European and American markets, and the tincture is recorded in European pharmacopoeia.

Preferably, the composition also comprises one or more of sage herb extract, thyme leaf extract, mulberry leaf extract, chrysanthemum extract, kudzu root extract, bupleurum extract, scutellaria extract, coptis extract, andrographis extract, honeysuckle extract, dandelion extract and azulene sodium sulfonate, and more preferably comprises all the components.

Wherein the dosage of each component in the composition is respectively as follows:

preferably, the composition comprises:

the invention surprisingly finds that the curative effect of the composition can be further obviously improved by adding one or more of the raw materials into the composition.

The sage extract is an alcohol extract of sage; salvia officinalis (Salvia officinalis) is a Labiatae, Salvia plant, annual, biennial or perennial herbaceous plant and evergreen shrub, and has high utilization value of leaves, stems and flowers. Has antiseptic, antibacterial, nerve strengthening, antipyretic, digestion promoting, intestine and stomach protecting, antiinflammatory, and immunity regulating effects. Aqueous and alcoholic extracts of sage leaves are effective and multifunctional natural antioxidants and free radical scavengers.

The alcohol extract or water extract of Thymus vulgaris extract, preferably Chinese medicinal Thymus vulgaris, is prepared by washing, drying, pulverizing, extracting with water or ethanol, concentrating, spray drying, and making into powder. The thyme extract has good antifungal activity and also has certain antiinflammatory effect. Has certain bacteriostatic action on common bacteria, saccharomycetes and moulds in food. Can be used as bactericide and antiseptic.

The folium Mori extract is alcohol extract or water extract of folium Mori, preferably folium Mori, and is prepared by washing, drying, pulverizing, extracting with water or ethanol, concentrating, spray drying, and making into folium Mori extract powder. The main chemical components of mulberry leaf include flavanone, flavonoid glycoside, alkaloid, polysaccharide, sterol, volatile oil and vitamin. Pharmacological research shows that the mulberry leaves have the effects of reducing blood sugar and blood fat, resisting tumors and oxidation, enhancing immunity, resisting inflammation, bacteria, viruses, blood coagulation, expanding blood vessels and the like, and the mulberry leaf extract has a stronger effect of inhibiting common pathogenic bacteria in the oral cavity.

The flos Chrysanthemi extract is ethanol extract or water extract of flos Chrysanthemi, preferably flos Chrysanthemi, and is prepared by washing, drying, pulverizing, extracting with water or ethanol, concentrating, spray drying, and making into flos Chrysanthemi extract powder. The chrysanthemum is a traditional Chinese medicine commonly used in China, and has various pharmacological actions such as antibiosis, anti-inflammation, antioxidation, blood vessel relaxing, blood fat reduction, anti-tumor, lead expelling and the like. In addition, there are findings. Flos Chrysanthemi Indici (dried head-like inflorescence of Chrysanthemum indicum L. of Compositae) has broad-spectrum antibacterial, antiviral, oxygen free radical scavenging, and antiallergic effects. The flos Chrysanthemi Indici mainly contains flavone, terpenes and volatile oil, and contains polysaccharide, protein, amino acids, chlorophyll, vitamins, chrysanthemumol, flos Chrysanthemi Indici lactone, and multiple trace elements. The flavone, terpenoid and volatile oil have antibacterial, antiinflammatory, antiviral, antioxidant and immunoregulatory effects, and can be used for inhibiting Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus diphtheriae, Streptococcus, Bacillus dysenteriae, Escherichia coli, Bacillus typhi and influenza virus. Chlorophyll can inhibit bacteria, kill bacteria, remove oral odor, and refresh breath.

The radix Puerariae extract is alcoholic extract or water extract of radix Puerariae, preferably radix Puerariae, and is prepared by washing, drying, pulverizing, extracting with water or ethanol, concentrating, spray drying, and making into radix Puerariae extract powder. The effective components of radix Puerariae are mainly isoflavone and radix Puerariae saponin. Isoflavone compounds are also the main active ingredients and can be divided into glycosides and aglycones. The former includes puerarin, daidzin, genistin, etc.; the latter mainly refers to daidzein, genistein, coumestrol, isoliquiritigenin, etc. Wherein the puerarin has various biological effects such as neuroprotection, immunity enhancing, myocardial contractility enhancing, antioxidant stress resisting, antiinflammatory, antiallergic, microcirculation improving, etc

The bupleuri radix extract is alcoholic extract or water extract of bupleuri radix, preferably bupleuri radix, and is prepared by washing, drying, pulverizing, extracting with water or ethanol, concentrating, spray drying, and making into powder. Radix bupleuri has significant anti-inflammatory effect, and the anti-inflammatory effective component of radix bupleuri is saikosaponin. Saikosaponin has inhibitory effect on various inflammatory processes including inflammatory exudation, increase in capillary permeability, release of inflammatory mediators, migration of leukocytes, and proliferation of connective tissue. Bupleuri radix also has immunoregulatory, anti-tumor, tranquilizing, vascular smooth muscle relaxing, blood flow of abdominal viscera improving, and digestive system improving effects; radix bupleuri is often combined with other medicines in the aspect of analgesia to form a prescription, or is used for soothing liver and benefiting gallbladder, relieving spasm and analgesia, or is used for regulating qi and harmonizing stomach, and activating blood and relieving pain.

The scutellaria extract is alcohol extract or water extract of scutellaria, preferably scutellaria of Chinese medicine, and is made up by washing, drying, pulverizing, water-extracting or alcohol-extracting, concentrating and spray-drying to obtain the scutellaria extract powder.

The Coptidis rhizoma extract is alcoholic extract or water extract of Coptidis rhizoma, preferably Coptidis rhizoma, which is Chinese medicinal material, by washing, drying, pulverizing, extracting with water or ethanol, concentrating, spray drying, and making into Coptidis rhizoma extract powder. The Coptidis rhizoma is dried rhizome of Coptis chinensis Franch, Coptis deltoidea C.Y.Cheng et Hsiao or Coptisteta wall. The coptis root has the effects of resisting bacteria, viruses, endotoxins, protozoans and the like and can inhibit. Berberine in Coptidis rhizoma has inhibitory effect on various gram-positive and gram-negative bacteria. The berberine has obvious inhibition effect on helicobacter pylori in vitro, and has low price and good safety.

The herba Andrographitis extract is alcoholic extract or water extract of herba Andrographitis, preferably herba Andrographitis, and is prepared by washing, drying, pulverizing, extracting with water or ethanol, concentrating, spray drying, and making into powder. The cold nature and bitter taste of the traditional Chinese medicine are recorded in the first part of the 2015 pharmacopoeia, which has the effects of clearing away heat and toxic materials, cooling blood and relieving swelling. Can be used for treating common cold, fever, sore throat, aphtha, cough, diarrhea, dysentery, stranguria, carbuncle, suppurative sore, and snake and insect bite. The andrographis paniculata extract mainly contains lactone and flavonoid, the effective component mainly contains lactone, and the andrographis paniculata extract has good heat-clearing and anti-inflammation effects and is a good reputation of traditional Chinese medicine antibiotics. Modern pharmacological studies show that andrographolide has antiinflammatory, antitumor, antiviral, antioxidant, immunoregulatory and hepatoprotective effects. Experiments prove that the andrographis paniculata extract can treat oral ulcer.

The flos Lonicerae extract is alcohol extract or water extract of flos Lonicerae, preferably Chinese medicinal flos Lonicerae, and is prepared by washing, drying, pulverizing, extracting with water or alcohol, concentrating, spray drying, and making into flos Lonicerae extract powder. The water or ethanol extract of flos Lonicerae has wide pharmacological effects, including antiinflammatory, antipyretic, antibacterial, antivirus, antifungal, antiendotoxin, blood pressure lowering, liver protecting, gallbladder promoting, fertility resisting, antioxidant, hemostasis and immunoregulation. The honeysuckle plays roles of clearing heat and reducing internal heat, diminishing inflammation and relieving pain and removing halitosis in the toothpaste.

The herba Taraxaci extract is alcohol extract or water extract of herba Taraxaci, preferably herba Taraxaci, by washing, drying, pulverizing, extracting with water or alcohol, concentrating, spray drying the liquid, and making into herba Taraxaci extract powder. The herba Taraxaci is broad-spectrum antibacterial agent, and has antifungal, antiviral, antiinflammatory, antioxidant, antitumor, liver protecting, gallbladder promoting, gastrointestinal and blood sugar protecting, blood vessel function improving, immunity regulating, and antifatigue effects. Can inhibit the growth of oral Hp, treat recurrent oral ulcer, and eliminate halitosis in oral cavity; can be administered for treating gastric and duodenal ulcer.

The sodium azulene sulfonate is prepared by modification on the molecular structure of guaiazulene. The sodium azulene sulfonate has excellent effects of resisting inflammation, bacteria and allergy and promoting tissue regeneration, can promote healing of burn and scald wound surfaces and ulcer wound surfaces and granulation regeneration, has multiple effects of heat prevention, radiation protection, chapping prevention and the like, can be used for treating various diseases, has high safety, is widely applied to various pharmaceutical preparations, and particularly has remarkable effect on treatment and prevention of skin and oral diseases.

As a preferred embodiment of the present invention, there is provided a composition comprising the following components:

0.1 to 0.8 portion of melaleuca alternifolia essential oil,

0.2-0.8 parts of arnica extract;

or the like, or, alternatively,

the composition provided by the invention has good hemostatic and bacteriostatic effects.

The invention also provides application of the composition in any one technical scheme in preparation of a medicament or an oral care product for treating gingival bleeding and inhibiting oral bacteria. Wherein the oral care product comprises toothpaste, mouthwash, tooth powder, oral spray, oral ointment and the like.

The medicine or the oral care product takes the composition obtained by any one of the technical schemes as an active component.

Specifically, the invention provides toothpaste which comprises the following components in parts by weight:

active components:

0.01-2 parts of melaleuca alternifolia essential oil;

0.02-5 parts by weight of arnica extract;

auxiliary materials:

preferably, the toothpaste comprises the following components in parts by weight:

active components:

0.05-1 part by weight of melaleuca alternifolia essential oil;

0.08-3 parts of arnica extract;

auxiliary materials:

the abrasive is selected from one or more of silicon dioxide, hydrated calcium hydrophosphate, anhydrous calcium hydrophosphate, calcium carbonate, aluminum hydroxide, sodium chloride or calcium pyrophosphate;

the humectant is selected from one or more of sorbitol, glycerol, propylene glycol, polyethylene glycol-8 or butanediol;

the foaming agent is selected from one or more of sodium dodecyl sulfate, sodium lauroyl sarcosinate, sodium cocoyl glycinate, sodium acyl glutamate or sodium cocoyl methyl taurate;

the thickening agent is selected from one or more of cellulose and derivatives thereof, guar gum, sodium alginate, xanthan gum, guar gum hydroxypropyl trimethyl ammonium chloride, carrageenan or locust bean;

the sweetener is selected from one or more of saccharin sodium, sucralose, xylitol, sodium cyclamate, aspartame, stevioside or mogroside;

the essence is selected from one or more of vanilla essence, spearmint essence, mint essence or wintergreen mint essence;

the preservative is selected from one or more of benzoic acid and salts thereof, sorbic acid and salts thereof or parabens;

the appearance modifier is selected from one or more of titanium dioxide, food pigment, food color lake or tooth cleaning particle;

the solvent is selected from deionized water.

Preferably, the active component of the toothpaste further comprises any one or more of the following components:

as a preferred technical scheme of the invention, the invention provides the toothpaste, which comprises the following components in parts by weight of 100 parts:

or the like, or, alternatively,

the toothpaste provided by the invention can obviously reduce the number of oral bacteria and is very effective in treating tooth sensitivity.

The invention also provides a preparation method of the toothpaste, which comprises the following steps: mixing the above active components (composition) to obtain toothpaste.

The preparation method of the toothpaste comprises the following steps:

A. dissolving the active component in a humectant, and stirring at high speed to prepare a first mixed solution;

B. adding a thickening agent into the first mixed solution, and stirring to obtain a second mixed solution;

C. adding an appearance modifier into the second mixed solution, and stirring to obtain a third mixed solution;

D. adding the third mixed solution, the sweetening agent, the friction agent, the essence, the foaming agent and the preservative into the solvent, and stirring to obtain a fourth mixed solution;

E. continuously stirring the fourth mixed solution under the vacuum condition of 0.08-0.12MPa to prepare paste;

F. checking, aseptically filling into aluminum plastic composite tube, sealing, and making into toothpaste.

The invention also provides mouthwash which comprises the following components in parts by weight:

active components:

0.01-2 parts of melaleuca alternifolia essential oil;

0.02-5 parts by weight of arnica extract;

auxiliary materials:

the solvent is selected from deionized water;

the humectant is selected from one or more of sorbitol, propylene glycol, glycerol and polyethylene glycol-8;

the surfactant is selected from one or more of poloxamer 407, sodium lauryl sulfate, sodium lauroyl sarcosinate and sodium cocoyl methyl taurate;

the thickening agent is selected from one or more of cellulose, carbomer and carrageenan;

the edible essence is selected from one or more of flos Caryophylli, cortex Cinnamomi, flos Osmanthi Fragrantis, oleum Menthae Dementholatum, herb Hierochloes Adoratae essence, herba Menthae Rotundifoliae, herba Menthae Haplocalycis, and herba Menthae Dementholati;

the colorant is selected from one or more of green, blue and red.

More preferably, the active ingredients of the mouthwash further comprise the following ingredients:

the technical scheme of the invention has the following beneficial effects:

the invention utilizes different medicinal mechanisms of each component, fully exerts the synergistic interaction of the components, and ensures that the obtained composition can prevent and assist in treating tooth sensitivity and simultaneously can inhibit the formation of dental plaque. And the material is safe and suitable for production.

Detailed Description

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

Example 1

The present embodiment provides a composition, which comprises the following components in parts by weight:

0.01 of melaleuca alternifolia essential oil,

arnica herb extract 3.

Example 2

0.5 of melaleuca alternifolia essential oil,

arnica herb extract 0.8.

Example 3

melaleuca alternifolia essential oil 1,

arnica herb extract 5.

Example 4

melaleuca alternifolia essential oil 2,

arnica herb extract 0.02.

Example 5

example 6

example 7

example 8

example 9

The embodiment provides a toothpaste, the total weight part is 100 parts, and the formula is as follows:

example 10

example 11

example 12

example 13

example 14

example 15

example 16

example 17

The embodiment provides mouthwash, which comprises 100 parts of the following components in parts by weight:

example 18

example 19

example 20

example 21

example 22

example 23

comparative examples 1 to 5

Comparative examples 1-5 each provide a composition having the following formulation, in parts by weight:

comparative examples 6 to 10

Comparative examples 6-10 each provide a composition having the following formulation, in parts by weight:

comparative examples 11 to 14

Comparative examples 11-14 each provide a toothpaste having a total weight of 100 parts, and the formulation is as follows:

comparative examples 15 to 19

Comparative examples 15-19 each provide a toothpaste having a total weight of 100 parts, and the formulation is as follows:

comparative example 20

This comparative example provides a sterile liquid containing 3.8% strontium chloride (prepared by procedures conventional in the art).

Comparative example 21

This comparative example provides a toothpaste commercially available containing 3.8% strontium chloride, which has been recognized to have a tooth sensitivity-reducing effect and is one of the toothpastes having the best anti-sensitivity effect at present.

Test example 1

The test example provides bacteriostatic tests of the compositions provided in examples 1-4 and the compositions provided in comparative examples 1-5.

Test subjects: the compositions provided in examples 1 to 4 and comparative examples 1 to 5 were prepared into sterile solutions of the same concentration, respectively. (the preparation method is carried out according to the conventional operation in the field)

The test method comprises the following steps:

the following species were selected to be present in the oral cavity: helicobacter pylori, staphylococcus aureus, candida albicans, streptococcus mutans; selecting hydrolyzed casein (MH) broth and agar culture medium.

After the standard strains are subjected to passage pure culture, a ring of strains are respectively inoculated in MH broth culture medium in a constant temperature incubator at 37 ℃ by using an inoculating ring to be cultured for 6 hours, and the bacterial quantity is identified and counted by a turbidimetric method. Respectively taking different test bacterial liquids cultured for 6 hours in the same amount, respectively and uniformly coating the bacterial liquids on the surface of an MH agar plate by using an aseptic cotton swab, respectively absorbing circular filter paper sheets of the aseptic liquids in the same amounts of comparative examples 1-5, comparative example 20 and examples 1-4 by using aseptic tweezers after the circular filter paper sheets are slightly dried, pasting the circular filter paper sheets on the surface of the MH agar plate, culturing the circular filter paper sheets for 24 hours at 37 ℃ in an incubator for observing and measuring the diameter (mm) of a bacteriostatic ring, repeating the bacteriostatic experiment for 3 times, and obtaining the result in the table 1, wherein the bacteriostatic result is the average value of.

And (3) test results:

TABLE 1

The MH broth medium containing the same amount of the sterile solutions of comparative examples 1 to 5, 20 and examples 1 to 4 was inoculated with 0.05ml (10 ml) of test bacterial liquid of the same amount of each of the different bacterial species⁵CFU/ml) and inoculated with the same species as a control in sterile MH broth medium, the results as in table 2 were obtained after 24 hours of incubation at 37 ℃.

TABLE 2

"+ + + +" bacteria clearly grew; "+" bacteria reduced growth; "0" medium was taken after inoculation for sterile growth.

As can be seen from table 2, the replacement of melaleuca alternifolia essential oil with triclosan was less bacteriostatic than the combination of the present invention. In addition, the melaleuca alternifolia essential oil is safe when repeatedly contacted by mouth, has no risk of causing mutation and chromosome aberration, and has no irritation to oral mucosa and gingival membrane when being applied to products for oral cavity, so that the melaleuca alternifolia essential oil can be safely used in oral care products such as toothpaste and the like. However, triclosan is lipophilic, can be accumulated in organisms for a long time, and has a potential threat, and a great deal of literature is available at present to explore the possible toxic effect of triclosan on organisms. Therefore, the melaleuca alternifolia essential oil avoids the side effect of the western medicine component triclosan, and accords with the development trend that the special-effect oral care product returns to nature.

Test example 2

This test example provides bacteriostatic tests for the compositions provided in examples 5-8 and comparative examples 6-10.

Test subjects: the compositions provided in examples 5 to 8 and comparative examples 6 to 10 were prepared into sterile solutions of the same concentration, respectively. (the preparation method is carried out according to the conventional operation in the field)

The test method comprises the following steps:

the following species present in the oral cavity were selected: helicobacter pylori, staphylococcus aureus, candida albicans, streptococcus mutans; selecting hydrolyzed casein (MH) broth and agar culture medium.

After the standard strains are subjected to passage pure culture, a ring of strains are respectively inoculated in MH broth culture medium in a constant temperature incubator at 37 ℃ by using an inoculating ring to be cultured for 6 hours, and the bacterial quantity is identified and counted by a turbidimetric method. Respectively taking different test bacterial liquids cultured for 6 hours in the same amount, respectively and uniformly coating the bacterial liquids on the surface of an MH agar plate by using an aseptic cotton swab, respectively absorbing circular filter paper sheets of the aseptic liquids of comparative examples 6-10 and examples 5-8 in the same amount by using aseptic tweezers after the bacterial liquids are slightly dried, attaching the circular filter paper sheets on the surface of the MH agar plate, culturing for 24 hours in an incubator at 37 ℃, observing and measuring the diameter (mm) of a bacteriostatic ring, repeating the bacteriostatic experiment for 3 times, wherein the bacteriostatic result is the average value of 3 experiments, and obtaining the results in the table 3.

And (3) test results:

TABLE 3

The results shown in Table 4 were obtained after culturing the MH broth medium containing the same amount of the sterile solutions of comparative examples 6 to 10 and examples 5 to 8 in an incubator at 37 ℃ for 24 hours, by inoculating 0.05ml (105CFU/ml) of each test bacterial suspension of the same amount of different bacterial species, and inoculating the same bacterial species in the sterile MH broth medium as a control.

TABLE 4

"+ + + +" bacteria clearly grew; "+" bacteria reduced growth; "0" is the medium grown aseptically.

The above results show that one or more of the medicinal sage, the thyme leaf extract, the mulberry leaf, the chrysanthemum, the kudzuvine root, the radix bupleuri, the radix scutellariae, the coptis root, the common andrographis herb, the honeysuckle, the dandelion and the azulene sodium sulfonate are further added, so that the obtained invention can obtain more remarkable effect, namely, the synergistic effect is achieved between one or more of the medicinal sage, the thyme leaf extract, the mulberry leaf, the chrysanthemum, the kudzuvine root, the radix bupleuri, the radix scutellariae, the coptis root, the common andrographis herb, the honeysuckle, the dandelion and the azulene sodium sulfonate and the composition consisting of the melaleuca alternifolia essential oil and the arnica herb.

Test example 3

This test example provides experimental verification that the toothpastes provided in examples 9-12 and comparative examples 11-14 are sensitive to teeth.

The inclusion criteria for the test subjects were: the teeth of people aged 18-65 years are more than or equal to 16 teeth, and the people have no systemic diseases and are simply sensitive to teeth, and when the teeth are stimulated by cold, heat, acid, sweet and mechanical actions (friction and hard biting), the people suddenly generate ache feeling, or suddenly feel or temporarily continue, and when the stimulation is removed, the ache feeling disappears. The experimental subjects participated in the human body trial test of the toothpaste, the experimental subjects were randomly grouped according to the number of test samples, 25 persons were used in each group, the toothpastes in the examples and comparative examples were used once a day, 10 days was a treatment course, and 20 days in total, namely two treatment courses.

The therapeutic effect judgment criteria of the test effect are as follows:

the effect is shown: after two treatment courses, the tooth sensitivity is controlled, and no relapse occurs after 20 days.

The method has the following advantages: tooth sensitivity was significantly improved and occurred to a significantly lesser degree than before the use of the experimental toothpaste.

And (4) invalidation: the tooth sensitivity degree is not obviously improved.

Total effective rate (significant + effective)/total number of cases × 100%.

The results are shown in Table 5.

TABLE 5

Test example 4

This test example provides experimental verification that the toothpastes provided in examples 13-16, comparative examples 15-19, and comparative example 21 are sensitive to teeth.

The therapeutic effect judgment criteria of the test effect are as follows: the criteria for therapeutic effect are as follows:

the effect is shown: after two treatment courses, the tooth sensitivity is controlled, and no relapse occurs after two weeks.

And (4) invalidation: the tooth sensitivity degree is not obviously improved.

Total effective rate (significant + effective)/total number of cases × 100%.

The results are shown in Table 6.

TABLE 6

Comparative example 21 has an anti-sensitivity effect comparable to that of example 14 provided by the present invention, but comparative example 21 provides a toothpaste containing strontium chloride. Strontium chloride is a common salt, but it is known in the art that it is a low toxic species and is somewhat irritating. Contact with eyes and mouth is avoided, and inhalation is avoided. Therefore, although the amount added in comparative example 19 is only 3.8%, it is still a low-toxicity product. However, the composition disclosed by the invention is prepared from natural plant source components, is green, natural and safe, and has no toxic or side effect.

Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. The toothpaste is characterized by comprising the following active ingredients:

2. the toothpaste according to claim 1, further comprising:

the solvent is selected from deionized water.

3. A method of preparing a toothpaste according to claim 1 or 2 comprising the steps of:

E. the fourth mixed solution is continuously stirred under the vacuum condition of 0.08-0.12MPa to prepare paste.