WO2024016109A1

WO2024016109A1 - Oral care composition of tea active substance and method for preparing same

Info

Publication number: WO2024016109A1
Application number: PCT/CN2022/106245
Authority: WO
Inventors: 温廷益; 张芸; 李翠平; 徐佩佩
Original assignee: 南京中科游子生物技术研究院有限公司
Priority date: 2022-07-18
Filing date: 2022-07-18
Publication date: 2024-01-25

Abstract

The present application pertains to the field of oral hygiene and care, and particularly provides a composition for cleaning the inner surface of the oral cavity and inhibiting bacteria thereon, comprising: 0.05-0.49 wt% of tea polyphenol, a surfactant, and 0.01-1.19 wt% of an inclusion compound that includes tea polyphenol, and comprising one or more of an auxiliary bacteriostatic agent, a refreshing substance, a flavoring agent, a pH regulator, an anti-freezing agent, and a preservative. The present application further provides a method for preparing the composition and use of the composition. The present application uses tea polyphenol, an active ingredient in tea, as the main bacteriostatic component, adds cleaning and auxiliary bacteriostatic components to ensure the effective bacteriostatic effect of the composition, and eliminates the irritating taste by means of component combination, thus having wide market prospects.

Description

Tea active substance oral care composition and preparation method thereof

Technical field

The present application relates to the field of oral care, and specifically to a composition for cleaning and inhibiting bacteria on the inner surface of the oral cavity and a preparation method thereof.

Background technique

The oral microbial community is a collection of microorganisms that colonize the human oral cavity, and most of them perform microbial physiological functions in the form of biofilms. When the ecological relationship between the microbial community and the host is imbalanced, a variety of oral infectious diseases can be induced, including caries, pulp apical disease, periodontal disease, wisdom tooth pericoronitis, jaw osteomyelitis, etc., seriously endangering oral health. . More importantly, the oral microbiome is closely related to systemic diseases such as oral tumors, diabetes, rheumatoid arthritis, cardiovascular disease, and premature birth. As the first line of defense of the human immune system, the oral mucosa is exposed to various pathogens (bacteria, viruses, etc.). Therefore, cleaning food residues in the oral cavity and inhibiting pathogenic bacteria are very important anti-infection methods. At present, most of the commonly used cleaning and antibacterial products for mucosal surfaces use chemical antibacterial agents, such as alcohol, metronidazole, chlorhexidine, sodium monofluorophosphate, benzalkonium bromide, and H ₂ O ₂ . However, chemicals are irritating to a certain extent and are not suitable for use by people with weak immunity (such as those suffering from immune system diseases), allergies, the elderly, pregnant and lactating women, children, and patients or animals with trauma to the mucosal surface. For example, alcohol can cause oral Drying, destroying the protective layer of mucus that keeps the mouth moist, and causing a stinging sensation; chlorhexidine has the risk of allergy. Secondly, long-term use of oral care products containing chlorhexidine will cause the oral mucosa and teeth to turn yellow. Fluoride may cause tooth deformity and softening, causing tooth enamel to lose its luster and become stained with yellow spots. In severe cases, it may also cause tooth hypoplasia and loss of normal shape. Therefore, there is an urgent need to develop a product with good antibacterial effect, broad spectrum, non-irritating, harmless to humans and animals, and not affected by the environment, organic matter, acid, alkali and other physical and chemical factors.

Contents of the invention

Tea is a unique product of China. Chinese people have a long history of drinking tea, and the national awareness of tea is very high. Research has found that the active ingredients in tea have broad-spectrum inhibitory effects on microorganisms (including bacteria, fungi and viruses). Based on this, this application aims to provide a composition for oral surface cleaning and antibacterial composition composed of tea extract as the main component, so as to solve the problems of irritation, sensitization and side effects existing in existing oral cleaning and antibacterial products. .

According to one aspect of the present application, a composition for cleaning and inhibiting bacteria on the inner surface of the oral cavity includes by weight: tea polyphenols 0.05-0.49wt%, surfactant, and inclusion complex containing tea polyphenols 0.01-1.19wt% .

Further, the inclusion compound in the composition is hydroxypropyl-β-cyclodextrin and the surfactant is an amino acid surfactant.

According to some embodiments in this application, the amino acid surfactant is selected from one or more of sodium lauroyl alanine, sodium cocoyl alanine, sodium lauroyl sarcosinate and any N-acyl amino acid salts. kind.

According to some embodiments in this application, the composition further includes one or more of an auxiliary bacteriostatic agent, a spirit-enhancing substance, a flavoring agent, a pH adjuster, an antifreeze agent, and a preservative.

Specifically, the auxiliary bacteriostatic agent includes one or more of amino acid surfactant 0.01-0.15wt%, berberine 0.005-0.010wt%, green tea extract 0.01-0.20wt%, lemon extract 0.01-0.20wt% ; Flavoring agents include sorbitol 2.00~8.00wt%, xylitol 0.05~2.00wt%, essence ≤3.00wt%, glycerol 2.00~24.00wt% and one or more substances that can improve the taste; improve The psychoactive substances include one or more of β-cyclodextrin-contained menthol 0.01-1.20wt%, tea polysaccharide 0.01-1.20wt%, and theanine 0.01-0.05wt%; the antifreeze is glycerol 2.00-1.20wt%. 24.00wt%; the preservative 0.20-0.40wt% includes one or more of sodium ascorbate, sodium benzoate, potassium sorbate, and methyl paraben; the pH adjuster includes sodium carbonate and/or sodium bicarbonate.

Further, berberine is 0.005-0.008wt%, green tea extract is 0.05-0.18wt%, lemon extract is 0.05-0.18wt%, and the pH is adjusted to 5.00-8.00 using a pH adjuster.

According to a certain embodiment of the present application, the composition includes, by weight: 0.05wt% tea polyphenols, 0.12wt% amino acid surfactant, and 0.01wt% hydroxypropyl-β-cyclodextrin.

According to another aspect of the present application, a method for preparing an oral surface cleaning and antibacterial composition is also provided, including the following steps:

Take a predetermined weight portion of plant active substances tea polyphenols, theanine, tea polysaccharide, β-cyclodextrin menthol and hydroxypropyl-β-cyclodextrin, and dissolve them in water to form solution 1;

Dissolve predetermined weight portions of glycerin, sorbitol, preservatives, and xylitol in water to form solution 2;

Add amino acid surfactant and essence to solution 2, mix evenly, then add solution 1, add water, adjust pH, and sterilize to obtain.

Further, preparing solution 1 also includes the following steps:

Add a predetermined weight portion of berberine; and/or

Add a predetermined weight portion of lemon extract; and/or

Add a predetermined weight portion of green tea extract.

Further, oral cleaning products comprising the above compositions and the use of any of the compositions in oral cleaning products.

The present application further provides a method for cleaning the oral cavity of humans or animals, by contacting any of the above compositions with the inner surface of the oral cavity.

Tea polyphenols are a type of polyhydroxy compounds contained in tea, mainly catechins (flavanols), flavones and flavonols, anthocyanins, phenolic acids and depsiphenolic acids, and polymeric phenols. A complex of similar compounds. Tea polyphenols have broad-spectrum and highly efficient antibacterial effects both in vivo and in vitro. Tea polyphenols exert their antibacterial effects mainly by specifically coagulating bacterial proteins, destroying bacterial cell membrane structures, and combining with bacterial DNA, thereby changing the physiological state of bacteria. The purpose of inhibiting its growth is that the antibacterial effect of tea polyphenols is very selective and can inhibit the growth of harmful bacteria and maintain the balance of normal flora. In addition, while tea polyphenols are antibacterial, they can also maintain the integrity of the oral mucosa, enhance the toughness and permeability of capillaries, enhance the resistance of blood vessels, quickly heal oral mucosal ulcers, reduce mucosal bleeding, and protect the oral mucosa.

Hydroxypropyl-β-cyclodextrin is a hydrophilic derivative formed by the condensation of β-cyclodextrin and 1,2-propylene oxide. Hydroxypropyl-β-cyclodextrin has a special three-dimensional ring structure with "hydrophobic inside and hydrophilic outside". The hydrophobic cavity can enclose the drug, thereby improving the water solubility, stability and antioxidant of the guest molecule. , resist photolysis, or achieve sustained release and three-dimensional separation effects. It is used to encapsulate tea polyphenols and reduce the bitterness caused by the combination of phenolic substances and proteins in the oral cavity through sustained release.

Tea polysaccharide is an acidic glycoprotein combined with a large number of mineral elements. The protein part is mainly composed of about 20 common amino acids. Tea polysaccharide has the effects of antioxidant, lowering blood sugar, lowering blood lipids, and regulating intestinal flora. It also has obvious effects in anticoagulation, preventing thrombosis, protecting blood phase, and enhancing the body's non-specific immunity.

Theanine is a unique free amino acid in tea. It is the main ingredient in tea that promotes body fluids and sweetness. It also has the health-care effects of relieving fatigue, relaxing the body and mind, lowering blood pressure and improving learning and memory abilities.

Tea polyphenols and berberine can also effectively prevent a variety of viral infections. Many studies have proven that berberine can reduce the risk of influenza virus and hepatitis B virus infection; tea polyphenols can inhibit various stages of the influenza virus growth cycle, and tea polyphenols also It can inhibit the attachment or invasion of viruses, such as SARS virus, human herpes virus, adenovirus, etc., by interacting with the receptor protein or envelope structure on the virus surface.

N-acyl amino acid salt surfactants such as sodium lauroyl sarcosinate, sodium lauroyl alanine and sodium cocoyl alanine are new green and environmentally friendly surfactants derived from renewable substances. They are different from other traditional surfactants. In comparison, sodium lauroyl sarcosinate, sodium lauroyl alanine, and sodium cocoyl alanine have better wettability, foaming, antibacterial, anti-corrosion, and antistatic capabilities. N-acyl amino acid salt surfactants such as sodium lauroyl sarcosinate, sodium lauroyl alanine and sodium cocoyl alanine are non-toxic and harmless, mild and non-irritating to the oral cavity. The degradation products are amino acids and fatty acids, which are harmful to the oral cavity. There is basically no impact on the environment; amino acid surfactants use their surface activity and bacteriostasis at the same time to qualitatively adsorb at the interface to achieve cleaning, antibacterial, antitoxic and other effects. For example, N-acyl amino acid salt surfactants have good effects in the oral cavity. Inhibits the action of lactic acid bacteria that convert glucose into lactic acid. As an important ingredient in oral products, it also has good compatibility with other ingredients and has the effect of cleaning the mouth and freshening breath.

Beta cyclodextrin menthol can be directly dissolved in an aqueous system without ethanol to help dissolve it, giving it a refreshing mint feel without the irritation caused by alcohol.

Flavoring agents can further improve or shield the bitter taste and astringent irritation of tea polyphenols.

Preservatives can effectively protect tea polyphenols from oxidation and maintain the antibacterial effect of the product.

Invent beneficial effects

The tea polyphenols in the oral cleaning antibacterial composition provided by this application have broad-spectrum antibacterial effects, without the need to add other antibacterial agents, the ingredients are safe, and the taste is relieved through the effect of the auxiliary material hydroxypropyl-β-cyclodextrin irritating. At the same time, adding preservatives can solve the problem of the impact of tea polyphenols on the antibacterial effect after oxidation. In addition, this application further adds auxiliary antibacterial agents such as berberine, which enhance the effect of tea polyphenols in preventing various viral infections. The cleaning and antibacterial composition in this application is non-irritating to mucous membranes, has broad-spectrum antibacterial properties, low onset concentration, fast action, stable performance, is easily soluble in water, and is environmentally friendly. It has the same antibacterial properties as chemicals. It has the same bacterial effect, but it is more natural, healthier and more in line with the consumption habits of Chinese people.

Description of drawings

The drawings that form a part of this application are used to provide a further understanding of this application. The illustrative embodiments and descriptions of this application are used to explain this application and do not constitute an improper limitation of this application. In the attached picture:

Figure 1 is a schematic diagram of the antibacterial effect of Example 1 and Example 2 of the present application on Staphylococcus aureus;

Figure 2 is a schematic diagram of the antibacterial effect of Example 3, Example 4, Example 5 and Example 6 of the present application on Staphylococcus aureus;

Figure 3 is a schematic diagram of the antibacterial effect of Comparative Example 1 of the present application on Staphylococcus aureus;

Figure 4 is a schematic diagram of the antibacterial effect of Comparative Example 2 of the present application on Staphylococcus aureus;

Figure 5 shows the antibacterial effect of oral care product 1 on the market on Staphylococcus aureus;

Figure 6 shows the antibacterial effect of oral care product 2 on the market on Staphylococcus aureus;

Figure 7 shows the antibacterial effect of 3 oral care products on the market on Staphylococcus aureus;

Figure 8 shows the antibacterial effect of 4 oral care products on the market on Staphylococcus aureus;

Figure 9 shows the antibacterial effect of 5 oral care products on the market on Staphylococcus aureus;

Figure 10 shows the antibacterial effect of 6 oral care products on the market on Staphylococcus aureus.

Detailed ways

The specific embodiments of the present application will be described in more detail below with reference to the accompanying drawings and examples, so that the solutions of the present application and the advantages of each aspect thereof can be better understood. However, the specific embodiments and examples described below are for illustrative purposes only and are not intended to limit the application. This should not be understood to mean that the preparation method of the present application is limited to the methods listed above.

Reference herein to "an embodiment" means that a particular feature, structure or characteristic described in connection with the embodiment can be included in at least one embodiment of the present application. The appearances of this phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those skilled in the art understand, both explicitly and implicitly, that the embodiments described herein may be combined with other embodiments.

The present application will be described below with reference to specific embodiments.

Weigh and mix predetermined weight portions of tea polyphenols, hydroxypropyl-β-cyclodextrin, auxiliary bacteriostatic agents and energy-boosting substances, then fully dissolve them in deionized water to form solution 1;

Then take predetermined weight portions of antifreeze, flavoring agents and preservatives and dissolve them in deionized water to form solution 2;

Then, add amino acid surfactant and essence to solution 2, mix evenly, add the above solution 1, add water, adjust the pH with a pH adjuster, and undergo filtration and sterilization to obtain a cleaning and antibacterial solution for the inner surface of the oral cavity.

Example 1

The oral inner surface cleaning and antibacterial composition of this embodiment includes the following components:

The antibacterial substance is 0.05wt% tea polyphenols;

Excipients, including:

Weigh the following raw materials in predetermined weight portions: tea polyphenols 0.05wt%, theanine 0.01wt%, tea polysaccharide 0.01wt%, β-cyclodextrin menthol 0.01wt% and hydroxypropyl-β-cyclodextrin 0.01wt After % mixing, dissolve in deionized water to form solution 1;

Then take predetermined weight portions of glycerol 2.00wt%, sorbitol 8.00wt%, sodium benzoate 0.20wt%, sodium ascorbate 0.20wt%, and xylitol 0.05wt% and dissolve them in deionized water to form solution 2;

Add sodium lauroyl alanine 0.01wt% and peppermint essence 0.10wt% to solution 2, mix evenly and then add solution 1, add water, adjust the pH to 6.12 with sodium bicarbonate, and filter and sterilize to obtain the oral cavity Surface cleaning and bacteriostatic solution.

Example 2

The preparation process is the same as in Example 1.

The antibacterial substance is 0.05wt% tea polyphenols;

Excipients, including:

Example 3

The preparation process is the same as the above embodiment. When preparing solution 1, it also includes adding a predetermined weight portion of berberine and green tea extract.

The antibacterial substance is 0.49wt% tea polyphenols;

Excipients, including:

Example 4

The preparation process is the same as the above embodiment.

The antibacterial substance is 0.20wt% tea polyphenols;

Excipients, including:

Example 5

The preparation process is the same as the above embodiment. When preparing solution 1, it also includes adding a predetermined weight portion of lemon extract.

The antibacterial substance is 0.30wt% tea polyphenols;

Excipients, including:

Example 6

The preparation process is the same as the above embodiment.

The antibacterial substance is 0.10wt% tea polyphenols;

Excipients, including:

Comparative example 1

The preparation process is the same as the above embodiment.

The oral inner surface cleaning and antibacterial composition of this comparative example includes the following components:

The antibacterial substance is 0.04wt% tea polyphenols;

Excipients, including:

Comparative example 2

The preparation process is the same as the above embodiment.

The antibacterial substance is 0.50wt% tea polyphenols;

Excipients, including:

Antibacterial effect test one

The test samples are as follows: Examples 1-6, Control 1 (water, sorbitol, propylene glycol, PEG-40 hydrogenated castor oil, tea extract, dipotassium glycyrrhizate, menthol, sucralose, hydroxyphenyl Methyl ester, sodium benzoate, flavoring, CI 19140, CI 42090);

Control 2 (water, glycerin, propylene glycol, sorbitol, poloxamer 407, flavor (containing tea extract), saccharin sodium, cetylpyridinium chloride (0.075%), sodium fluoride (0.05%), Potassium sorbate, menthol, CI19140, CI42051);

Control 3 (water, glycerin, sorbitol, PEG-40 hydrogenated castor oil, xylitol, cherry blossom extract, yeast fermentation product extract, tea extract, peppermint extract, probiotics (paracheese milk) bacilli), menthol, citric acid, methylparaben, chlorogalactose, domiphene, flavor);

Control 4 (water, sorbitol, glycerin, sodium chloride, methyl paraben, sodium benzoate, essence, menthol, lactobacilli, lysozyme, plant peptides, propolis extract, honeysuckle flower extract, Bletilla striata Root extract, Dendrobium officinale extract, hydrogenated castor oil, citric acid);

Control 5 (water, ethanol, sorbitol, thymol, eucalyptol, methyl salicylate, menthol, sodium benzoate, benzoic acid, poloxamer 407, sodium saccharin, CI 42053, food flavors);

Control 6 (contains 0.6 g of chlorhexidine gluconate and 0.1 g of metronidazole per 500 ml; excipients are: glycerin, polysorbate 80, polyethylene glycol-400, concentrated mint water, ethanol, saccharin sodium, pigment, essence, purified water).

Test strain: Staphylococcus aureus ATCC6538

Test method: According to the antibacterial ring test requirements in the Disinfection Technical Specifications (2002 Edition), 4 test samples and 1 negative control sample are placed on each bacteria-contaminated plate, for a total of 5 pieces. Use sterile forceps to take a sample piece and place it on the surface of the plate. The centers of each sample should be at least 25mm apart and at least 15mm apart from the edge of the flat plate. After the patch is placed, use sterile tweezers to gently press the sample piece to make it stick to the surface of the plate. Cover the plate, place it in a 37°C incubator, and incubate for 16 to 18 hours to observe the results. Use a vernier caliper to measure the diameter of the inhibition ring (including the patch) and record it. The test was repeated three times. The bactericidal activity was evaluated based on the presence and size of the inhibition zone.

The test results are shown in Figure 1-10, and the statistics are as follows:

Table 1 Antibacterial ring test results

According to the results shown in the above table, combined with those shown in Figures 1-10, CK all represent sterile water, and the numbers represent examples.

Note: According to the standard, if the diameter of the inhibitory ring is greater than 7mm, it is judged to have antibacterial effect; if the diameter of the inhibitory ring is less than or equal to 7mm, it is judged to have no antibacterial effect.

Antibacterial effect test 2

Test samples: Examples 1-6, Comparative Examples 1-2, Control 3 (water, glycerol, sorbitol, PEG-40 hydrogenated castor oil, xylitol, cherry blossom extract, yeast fermentation product extract , tea extract, peppermint extract, probiotics (Lactobacillus paracasei), menthol, citric acid, methyl paraben, chlorogalactose, domiphene, flavor);

Control 6 (contains 0.6 g of chlorhexidine gluconate and 0.1 g of metronidazole per 500 ml. Excipients are: glycerin, polysorbate 80, polyethylene glycol-400, concentrated mint water, ethanol, saccharin sodium, pigment, Fragrance, purified water).

Test strain: Staphylococcus aureus ATCC6538

Test method: According to the GB 15979-2002 Hygienic Standard for Disposable Hygiene Products, C4 dissolution antibacterial (anti)bacterial product antibacterial performance test method, each test sample acts on Staphylococcus aureus for 2min, 5min, 10min, 20min, calculated The antibacterial rates are as follows:

Table 2 Antibacterial rate test results

Note: According to the standard antibacterial rate of 50% to 90%, the product has antibacterial effect. The antibacterial rate is greater than or equal to 90%, and the product has strong antibacterial effect.

Taste test

Test samples: Examples 1-6, Comparative Examples 1-2.

Test Methods:

Using the public opinion survey scoring method, 100 people aged 20 to 70 years old (gender is not limited) were selected as trial subjects, and the test samples were tried separately to evaluate the taste of the samples.

Grading:

Total score 5

A score of 4-5 means the mouthwash tastes comfortable, has a suitable tea flavor, is mild and non-irritating;

A score of 3-4 indicates that the mouthwash tastes average and has the obvious bitterness of tea, which is within an acceptable range;

A score of less than 3 means that the mouthwash has a distinctly bitter taste and is unacceptable.

Test Results:

As shown in the following table:

Table 3 Oral care composition taste test score result interval number of people proportion results

测试对象Test object	4-5分4-5 points	3-4分3-4 points	3分以下3 points or less
实施例1Example 1	86％86%	14％14%	0％0%
实施例2Example 2	92％92%	8％8%	0％0%
实施例3Example 3	63％63%	28％28%	9％9%
实施例4Example 4	61％61%	25％25%	14％14%
实施例5Example 5	80％80%	16％16%	4％4%
实施例6Example 6	89％89%	11％11%	0％0%
对比例1Comparative example 1	77％77%	23％twenty three%	0％0%
对比例2Comparative example 2	66％66%	13％13%	21％twenty one%

Summary: The cleaning and antibacterial composition provided in this application can achieve an antibacterial effect close to that of chemical products by relying only on the natural active substance tea polyphenols in tea. It can prevent the occurrence of dental caries and periodontitis by inhibiting bacterial growth. It is safe and has no side effects. It inhibits bacteria and does not cause harm to the oral mucosa. Tea polyphenols and berberine also have certain antiviral effects, which can reduce the risk of viral infection from the oral cavity to a certain extent, enabling oral care compositions to effectively protect against influenza virus, coronavirus, hepatitis B virus, human herpes virus and other viruses. Prevention of infection; adding sodium lauroyl alanine, sodium cocoyl alanine, and sodium lauroyl sarcosinate surfactants can better clean the mouth and freshen breath and assist tea polyphenols in antibacterial; in addition, use hydroxypropylene Base-β-cyclodextrin wraps tea polyphenols and adds a variety of flavoring agents to eliminate the bitterness caused by tea polyphenols, and then adds β-cyclodextrin menthol, tea polysaccharide, and theanine to make the overall taste mild and fragrant, and also adds It can refresh the mind. Finally, by adding preservatives, the problem that tea polyphenols are easily oxidized during use and affects the antibacterial effect is solved.

Since β-cyclodextrin menthol can be directly dissolved in an aqueous phase system without ethanol as a solubilizer, the composition in this application is an alcohol-free formula and has a refreshing mint feeling without the irritation caused by alcohol. It completely solves the problems of poor safety and irritating taste of existing chemical products, and is suitable for preparing antibacterial, safe and natural oral care products with suitable taste.

Obviously, the above-mentioned embodiments are only examples to clearly illustrate the present application, and are not intended to limit the implementation. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. An exhaustive list of all implementations is neither necessary nor possible. The obvious changes or changes derived therefrom are still within the protection scope of this application.

Claims

A composition for cleaning and inhibiting bacteria on the inner surface of the oral cavity, which is characterized in that, according to the weight fraction of the composition, it includes: 0.05 to 0.49 wt% of tea polyphenols, surfactants, and inclusions of tea polyphenols. Material 0.01～1.19wt%.
The composition according to claim 1, wherein the inclusion compound is hydroxypropyl-β-cyclodextrin; and the surfactant is an amino acid surfactant.
The composition according to claim 2, wherein the amino acid surfactant is selected from the group consisting of sodium lauroyl alanine, sodium cocoyl alanine, sodium lauroyl sarcosinate and any N-acyl amino acid salt One or more of the categories.
The composition according to claim 1, further comprising one or more of an auxiliary bacteriostatic agent, a spirit-enhancing substance, a flavoring agent, a pH adjuster, an antifreeze agent, and a preservative.
The composition according to claim 4, wherein the auxiliary bacteriostatic agent includes amino acid surfactant 0.01-0.15wt%, berberine 0.005-0.010wt%, green tea extract 0.01-0.20wt%, lemon extract One or more substances 0.01 to 0.20wt%; the flavoring agents include sorbitol 2.00 to 8.00wt%, xylitol 0.05 to 2.00wt%, essence ≤ 3.00wt%, glycerol 2.00 to 24.00wt% and One or more of any substances that can improve the taste; the spirit-boosting substances include 0.01 to 1.20 wt% of β-cyclodextrin-contained menthol, 0.01 to 1.20 wt% of tea polysaccharide, and 0.01 to 0.01 to 1.20 wt of theanine. One or more of 0.05wt%; the antifreeze is glycerol 2.00~24.00wt%; the preservative 0.20~0.40wt% includes one of sodium ascorbate, sodium benzoate, potassium sorbate, and methyl paraben. One or more; pH adjuster includes sodium carbonate and/or sodium bicarbonate.
The composition according to claim 5, wherein the berberine is 0.005-0.008wt%, the green tea extract is 0.05-0.18wt%, and the lemon extract is 0.05-0.18wt%.
According to the composition of any one of claims 4-6, the pH adjuster adjusts the pH to 5.00-8.00.
The composition according to claim 1, characterized in that, according to the weight fraction of the composition, it includes: tea polyphenols 0.05wt%, amino acid surfactant 0.12wt%, hydroxypropyl-β-cyclodextrin 0.01 wt%.
A method for preparing a composition for cleaning and inhibiting bacteria on the inner surface of the oral cavity, which is characterized by comprising the following steps:

Take a predetermined weight portion of the plant active substances tea polyphenols, theanine, tea polysaccharide, β-cyclodextrin menthol and hydroxypropyl-β-cyclodextrin, and dissolve them in water to form solution 1;

Dissolve predetermined weight portions of glycerin, sorbitol, preservatives, and xylitol in water to form solution 2;

Add amino acid surfactant and essence to solution 2, mix evenly, then add solution 1, add water, adjust pH, and sterilize to obtain.
The preparation method according to claim 9, characterized in that the solution 1 further includes:

Add a predetermined weight portion of berberine; and/or

Add a predetermined weight portion of lemon extract; and/or

Add a predetermined weight portion of green tea extract.
An oral cleaning product comprising the composition of any one of claims 1-6.
Application of the composition according to any one of claims 1 to 6 in oral cleaning products.
A method for cleaning the oral cavity of humans or animals, characterized by contacting the composition of any one of claims 1 to 6 with the inner surface of the oral cavity of humans or animals.