CN108450417A - Application of the absorbent cotton on dichocrocis punctiferalis ovum collecting - Google Patents
Application of the absorbent cotton on dichocrocis punctiferalis ovum collecting Download PDFInfo
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- CN108450417A CN108450417A CN201810099144.5A CN201810099144A CN108450417A CN 108450417 A CN108450417 A CN 108450417A CN 201810099144 A CN201810099144 A CN 201810099144A CN 108450417 A CN108450417 A CN 108450417A
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- Prior art keywords
- absorbent cotton
- ovum
- dichocrocis punctiferalis
- hydromel
- breeding cage
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses application of the absorbent cotton on dichocrocis punctiferalis ovum collecting, belong to technical field of insect feeding.The invention also discloses the methods for collecting dichocrocis punctiferalis ovum using absorbent cotton, upper hydromel mainly will be dipped in 4 sizable absorbent cotton in side of imago breeding cage, it is fixed on the inside of 4 sides of imago breeding cage, the absorbent cotton removed with gauze wrapped when collection, the hydromel on absorbent cotton is rinsed well with tap water, and impregnated in liquor natrii hypochloritis, it dries in the air to half-dried, is placed in plastics freshness protection package and is used for squamous subculture.The absorbent cotton of hydromel is moistened in the present invention can not only attract imago feeding to supplement the nutrients, and lure that it lays eggs on absorbent cotton into, before solving the problems, such as with the methods of gauze be difficult to collect dichocrocis punctiferalis ovum and caused by waste, it collects the quantity that the quantity of dichocrocis punctiferalis ovum is far above the dichocrocis punctiferalis ovum collected using the methods of gauze, and collecting effect significantly improves；Secondly, absorbent cotton can also reuse, and reduce cost.
Description
Technical field
The invention belongs to insect method for breeding fields, and in particular to purposes of the absorbent cotton on dichocrocis punctiferalis ovum collecting also relates to
And the method using absorbent cotton collection dichocrocis punctiferalis ovum.
Background technology
Dichocrocis punctiferalis (Conogethes punctiferalis) is commonly called as peach phycitid, peach borer, the wild snout moth's larva of peach moth etc., belongs to squama
The wild snout moth's larva category of wing mesh Pyralidae moth, mainly cause harm Chinese chestnut, peach, Lee, pears, apple, pomegranate, plum, cherry, citrus, loquat, apricot, persimmon, mountain
A variety of fruit trees such as short, bristly hair or beard, masson pine, China fir, sunflower, soybean, corn, cotton and crops, feeding habits are miscellaneous, and host range is wide, are one
Kind important omnivorousness and borer pest (the plant protection such as Wang Zhenying, 2006, (2):67-69).In recent years, due to weather,
The change of cropping system and planting type, dichocrocis punctiferalis population are in swift and violent ascendant trend.Dichocrocis punctiferalis larva multigroup on maize ear
It is poly- to endanger, can there are the harm of bull larva, moth food corn kernel on same fringe, and cause serious ear rot, while stem can be eaten into, cause
Plant falls to roll over, and seriously affects the quality and yield of corn ear, it has also become the another corn field Important Agricultural after corn borer
Pest.
The indoors artificial continuously rearing of dichocrocis punctiferalis is to carry out biology, Physiology and biochemistry, ecology, pesticide screening test, biology to prevent
The basis for controlling equal area researches needs big more particularly to carrying out in a deep going way for the research work such as biological control and drug resistance measurement
Measure selected insect source.Dichocrocis punctiferalis ovum dissipates production, granular, has several to be sticked together once in a while, ovum grain not easily collecting, therefore the production of dichocrocis punctiferalis
Ovum and collection are to first have to solve the problems, such as, only obtain being smoothed out for more ovum amounts ability guarantee tests.It collects at present
The tool of dichocrocis punctiferalis ovum is mainly to be laid eggs for dichocrocis punctiferalis with the fresh griggles for being surrounded by one layer of cotton gauze, and single female eggs on average amount is most
55 a height of (the insect journals such as Du Yanli, 2012, (5)：561-569).When using oviposition material of the gauze as dichocrocis punctiferalis, remove
Outside gauze, it is also attached with ovum grain on smooth culture dish and imago cage, not easily collecting, wastes (the Xu Li honor China agriculture of ovum amount
Industry academy of sciences master thesis, 2011).Therefore, the negligible amounts of dichocrocis punctiferalis ovum collected by existing method, there is an urgent need for improve.
Invention content
It is de- present invention aims at providing in order to solve the problems, such as that quantity present on dichocrocis punctiferalis ovum collecting is few and ovum wastes
Purposes of the fat cotton on dichocrocis punctiferalis ovum collecting.
Another object of the present invention is to provide the method for collecting dichocrocis punctiferalis ovum using absorbent cotton.
To achieve the above object, technical scheme is as follows：
The present invention provides application of the absorbent cotton on dichocrocis punctiferalis ovum collecting.
Absorbent cotton described in above application refers to the chemically treated cotton for removing fat, is mingled with through removing by raw cotton
Object, degreasing, bleaching, washing, drying, arrangement are processed into.
The absorbent cotton is commercially available.
The present invention also provides the methods for collecting dichocrocis punctiferalis ovum using absorbent cotton, include the following steps：
(1) in the bottom culture dish holding of imago breeding cage, and the sea for being moistened with 5~15% hydromels is put into culture dish
Continuous or absorbent cotton；The dichocrocis punctiferalis pupa of interior energy emergence in 12 hours or the adult pairing sprouted wings in 12 hours are put into imago breeding cage；
(2) the 3rd~4 day be put into after pupa or adult, will be whole with the sizable absorbent cotton in 4 sides of imago breeding cage
5~15% hydromels are dipped in, then absorbent cotton whole face is individually fixed in the inside of 4 sides of imago breeding cage, per all over the world
Noon covers tightly the top of imago breeding cage with wet towel, and the next morning removes towel；
(3) a dichocrocis punctiferalis ovum is collected within every 2 days, remove the absorbent cotton for being attached with dichocrocis punctiferalis ovum in the morning, changes and new is moistened with 5
The absorbent cotton of~15% hydromel；The absorbent cotton removed with gauze wrapped when collection, then gently will be on absorbent cotton with tap water
Hydromel rinse well, place into a concentration of 0.5~1.5% liquor natrii hypochloritis and impregnate 5~15 seconds, finally with pure
Water is rinsed to no sodium hypochlorite taste, and is dried in the air to half-dried, and gauze is removed, by absorbent cotton be placed in plastics freshness protection package hatching for after
Generation raising, or put it into 7~10 DEG C of refrigerators and save backup.
The specification for described in above method step (1) being imago breeding cage is 45cm × 31cm × 28cm or 40cm × 20cm
× 26cm etc..Commercially available or customization obtains.
A diameter of 8~12cm of culture dish described in above method step (1).
The concentration of hydromel described in above method step (1) or (2) is preferably 10%.
The thickness of absorbent cotton described in above method step (2) is 0.3~0.7cm.
Fixation described in above method step (2) refers to being fixed with paper clip or other tools.
Rearing conditions described in above method step (1) or (2) are：23~28 DEG C of temperature, relative humidity 65~75%.
The concentration of liquor natrii hypochloritis is preferably 1.0% described in above method step (3).
Compared with the prior art, the present invention has the advantage that and has the beneficial effect that：(1) present invention, which uses, is moistened with the de- of hydromel
Fat cotton collection dichocrocis punctiferalis ovum effect is good, collects the female egg laying amount of quantity average out to list 223.3 of dichocrocis punctiferalis ovum, far above utilization
The quantity 148.67 for the dichocrocis punctiferalis ovum that the methods of gauze is collected；(2) hydromel on absorbent cotton of the present invention can attract adult
Feeding supplements the nutrients, and lays eggs on absorbent cotton, avoids caused by being difficult to the dichocrocis punctiferalis ovum collected with the methods of gauze in the past
Waste；(3) absorbent cotton can reuse, and reduce cost.
Specific implementation mode
The present invention will be further explained by the following examples, but is not constituted to the scope of the present invention
Any restrictions.
Application of 1 absorbent cotton of embodiment in dichocrocis punctiferalis imago breeding
(1) under the indoor conditions that temperature is 25 DEG C, relative humidity is 70%, one is put into directly in imago breeding cage bottom portion
Diameter is the culture dish of 10cm, the sponge for being moistened with 10% hydromel is put into culture dish, for dichocrocis punctiferalis Adult supplement nutrient；It is small by 12
When interior emergence the pairing of dichocrocis punctiferalis adult be put into the imago breeding cage that specification is 45cm × 31cm × 28cm, it is right per cage 50；.
(2) it is put into the 4th day after adult, will integrally be dipped in 4 sizable absorbent cotton in side of imago breeding cage upper dense
Degree is 10% hydromel, then its whole face is fixed on to the inside of 4 sides of imago breeding cage, the degreasing with paper clip
The thickness of cotton is 0.5cm；Every afternoon covers tightly imago breeding cage top portion with wet towel, and the next morning removes towel；
(3) a dichocrocis punctiferalis ovum is collected within every 2 days, remove the absorbent cotton for being attached with dichocrocis punctiferalis ovum in the morning, changes and new is moistened with 5
The absorbent cotton of~15% hydromel；The absorbent cotton removed with gauze wrapped when collection, then gently will be on absorbent cotton with tap water
Hydromel rinse well, place into and impregnate 10 seconds in a concentration of 1% liquor natrii hypochloritis, finally with pure water rinse to
Without sodium hypochlorite taste, gauze is removed, dries in the air to half-dried be placed in plastics freshness protection package and hatches for continuously rearing.Statistical collection
Dichocrocis punctiferalis ovum quantity.
As a result 13000, dichocrocis punctiferalis ovum, average single female egg laying amount 260 are obtained altogether.It is significantly higher than and existing is received with gauze
Dichocrocis punctiferalis ovum quantity 38.2-54.9 (Xu Li honor Chinese Academy of Agricultural Sciences master thesis, 2011) of collection, illustrates this hair
The bright effect that dichocrocis punctiferalis ovum is collected with the absorbent cotton for dipping in hydromel is good.
2 present invention of embodiment collects the contrast test of dichocrocis punctiferalis ovum using absorbent cotton
(1) selected insect source：Inst. of Plant Protection, Hebei-Prov. Academy of Agricultural and Forestry Scie is come from for examination dichocrocis punctiferalis.
(2) experiment of dichocrocis punctiferalis ovum is collected with gauze：It is put in imago breeding cage (specification is 45cm × 31cm × 28cm) bottom
The culture dish for entering a diameter 10cm is put into the sponge for being moistened with 10% hydromel in culture dish, for dichocrocis punctiferalis adult Replacement Battalion
It supports.The dichocrocis punctiferalis adult to sprout wings in 12 hours pairing is put into rearging cage, it is right per cage 50.One piece is put in imago breeding cage top portion
With an equal amount of gauze in top, spawning place is provided for dichocrocis punctiferalis, gauze upper cover one opens paraffin paper, plays water proof, paraffin paper
On repave a wet towel moisturizing, replace wet towel daily, the every 2 days dichocrocis punctiferalis ovum collected on a gauze more renew gauze,
Until experiment proceeds to the whole death of dichocrocis punctiferalis female adult.Egg laying amount is recorded, the total egg laying amount of 50 pairs of dichocrocis punctiferalis adults, single female production are calculated
Ovum amount and hatching rate, are repeated 3 times.Above-mentioned rearing conditions are 25 DEG C, the indoor conditions of relative humidity 70%.
(3) experiment of dichocrocis punctiferalis ovum is collected with absorbent cotton：In imago breeding cage (specification is 45cm × 31cm × 28cm) bottom
It is put into the culture dish of a diameter 10cm, the sponge for being moistened with 10% hydromel is put into culture dish, is supplemented for dichocrocis punctiferalis adult
Nutrition.The dichocrocis punctiferalis adult to sprout wings in 12 hours pairing is put into rearging cage, it is right per cage 50.The absorbent cotton of thickness 0.5cm is taken,
It is cut into the size of 4 sides of imago breeding cage, integrally dips in 10% hydromel, for inducing dichocrocis punctiferalis imago feeding to supplement
Nutrition simultaneously provides spawning place；The absorbent cotton whole face for being moistened with hydromel is fixed on to 4 sides of imago breeding cage with paper clip
Inside, cover tightly imago breeding cage top portion with wet towel every afternoon, the next morning removes towel, collects a peach within every 2 days
Snout moth's larva ovum is eaten into, what is more renewed is moistened with the absorbent cotton of 10% hydromel, the absorbent cotton record egg laying amount removed；Gauze wrapped when collection
Mulse on absorbent cotton is gently washed down with tap water, is then placed in 1% liquor natrii hypochloritis and impregnates 10 by the absorbent cotton removed
Second, it is finally rinsed to no sodium hypochlorite taste with pure water, and dry in the air to half-dried, is subsequently placed in wait in plastics freshness protection package and incubate
Change, until experiment proceeds to the whole death of dichocrocis punctiferalis female adult.It calculates the total egg laying amount of 50 pairs of dichocrocis punctiferalis adults, single female egg laying amount and incubates
Rate is repeated 3 times.Above-mentioned experiment is in the indoor progress that temperature is 25 DEG C, relative humidity is 70%.
As a result (being shown in Table 1), the average single female egg laying amount of the quantity that dichocrocis punctiferalis ovum is collected with the absorbent cotton for dipping in hydromel of the invention is
223.33, and the female egg laying amount of list 148.67 on gauze, the former illustrates to use 74.66 more than the latter singly female egg laying amount
The absorbent cotton collection dichocrocis punctiferalis ovum that the present invention dips in hydromel is obviously better than with gauze effect, solves ovum grain not easily collecting and waste
The problem of, and absorbent cotton can also reuse, and save cost.Secondly, the hatching rate of 2 kinds of collection methods does not have conspicuousness poor
It is different.Illustrate that the ovum on absorbent cotton passes through hypochlorite disinfectant, to the no harmful effect of the hatching of dichocrocis punctiferalis ovum.Moreover, it has been found that
It can be seen that dichocrocis punctiferalis ovum grain, makes on the adult case wire netting of gauze processing, the filter paper gap of bottom layer pad, culture dish and sponge
It is wasted at ovum amount.And the absorbent cotton for dipping in hydromel can also induce dichocrocis punctiferalis imago feeding Replacement Battalion in addition to providing spawning place
It supports.
1 present invention of table collects dichocrocis punctiferalis ovum comparative test result with absorbent cotton
| Oviposition material | Total ovum amount (grain) | Single female egg laying amount (grain) | Hatching rate (%) |
| Gauze | 7433.33 | 148.67 | 85.67±2.19a |
| Dip in the absorbent cotton of hydromel | 11166.67 | 223.33 | 87.67±1.76a |
Note：Data are average value ± standard error in table, indicate same letter and indicate difference not significantly (P ＞ 0.05).
Claims (9)
1. application of the absorbent cotton on dichocrocis punctiferalis ovum collecting.
2. the method for collecting dichocrocis punctiferalis ovum using absorbent cotton, it is characterised in that include the following steps：
(1) in the bottom culture dish holding of imago breeding cage, and be put into culture dish the sponge for being moistened with 5~15% hydromels or
Absorbent cotton；The dichocrocis punctiferalis pupa of interior energy emergence in 12 hours or the adult pairing sprouted wings in 12 hours are put into imago breeding cage；
(2) the 3rd~4 day be put into after pupa or adult, will integrally dip in 4 sizable absorbent cotton in side of imago breeding cage
Then absorbent cotton whole face is individually fixed in the inside of 4 sides of imago breeding cage by 5~15% hydromels, use every afternoon
Wet towel covers tightly the top of imago breeding cage, and the next morning removes towel；
(3) collect a dichocrocis punctiferalis ovum within every 2 days, remove the absorbent cotton for being attached with dichocrocis punctiferalis ovum in the morning, change it is new be moistened with 5~
The absorbent cotton of 15% hydromel；The absorbent cotton removed with gauze wrapped when collection, then gently will be on absorbent cotton with tap water
Hydromel is rinsed well, is placed into 0.5~1.5% liquor natrii hypochloritis and is impregnated 5~15 seconds, is finally rinsed to nothing with pure water
Sodium hypochlorite taste, and dry in the air to half-dried, removal gauze, absorbent cotton, which is placed in hatching in plastics freshness protection package, is used for continuously rearing, or
Person puts it into 7~10 DEG C of refrigerators and saves backup.
3. according to the method described in claim 2, it is characterized in that the specification for described in its step (1) being imago breeding cage is
45cm × 31cm × 28cm or 40cm × 20cm × 26cm.
4. according to the method described in claim 2, it is characterized in that a diameter of 8~12cm of culture dish described in its step (1).
5. according to the method described in claim 2, it is characterized in that hydromel described in its step (1) or (2) it is a concentration of
10%.
6. according to the method described in claim 2, it is characterized in that the thickness of absorbent cotton described in its step (2) be 0.3~
0.7cm。
7. according to the method described in claim 2, it is characterized in that the fixation described in its step (2) refer to paper clip or its
He fixes tool.
8. according to the method described in claim 2, it is characterized in that the rearing conditions described in its step (1) or (2) are：Temperature
23~28 DEG C, relative humidity 65~75%.
9. according to the method described in claim 2, it is characterized in that liquor natrii hypochloritis's described in its step (3) is a concentration of
1.0%.
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| CN201810099144.5A CN108450417B (en) | 2018-02-01 | 2018-02-01 | Application of absorbent cotton in collecting eggs of dichocrocis punctiferalis |
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Cited By (1)
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| CN108450417B (en) | 2021-02-26 |
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