CN108377975A - A kind of richness calcium earthworm protein compound and its preparation method and application and by-product richness calcium wormcast - Google Patents
A kind of richness calcium earthworm protein compound and its preparation method and application and by-product richness calcium wormcast Download PDFInfo
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- CN108377975A CN108377975A CN201810131991.5A CN201810131991A CN108377975A CN 108377975 A CN108377975 A CN 108377975A CN 201810131991 A CN201810131991 A CN 201810131991A CN 108377975 A CN108377975 A CN 108377975A
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- calcium
- earthworm
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- richness
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Abstract
The present invention provides a kind of rich calcium earthworm protein compound and its preparation method and application and by-product richness calcium wormcasts；Belong to vermiculture processing technique field, the calcium content of the richness calcium earthworm protein compound is 240~360mg/kg.The preparation method of the richness calcium earthworm protein compound, includes the following steps：1) it is that basic material piles up rich calcium vermiculture bed with cow dung, calcium source and stalk；The content of calcium constituent is 120~240mg/kg in the richness calcium vermiculture bed；2) earthworm kind is seeded in the rich calcium vermiculture bed, cultivates and obtains within 30~45 days rich calcium earthworm；3) by the rich rich calcium earthworm protein compound of the broken acquisition of calcium dry earthworm.On the basis of containing abundant earthworm protein, the also microelements of calcium containing high level can be widely applied for rich calcium animal feed, food and health products, improves the added value of earthworm richness calcium earthworm protein compound of the present invention.
Description
Technical field
The invention belongs to vermiculture processing technique fields, and in particular to a kind of richness calcium earthworm protein compound and its preparation
Methods and applications and by-product richness calcium wormcast.
Background technology
Currently, the cultural technique of earthworm comparative maturity, such as application No. is CN201310047641.8, application No. is
CN201610206687.3, the cultivation skill that earthworm is disclosed application No. is the Chinese invention patent of CN201510747429.1
Art, these methods generally are laid on soil to be used as and support by the animal wastes such as cow dung, pig manure and straw, straw etc. for feed
Bed is grown to be cultivated.
Calcium (Ca) is primarily present in as the indispensable element of human body in bone and tooth.Minerals in human body account for about body
The 5% of weight, the 2% of calcium about percentage of liveweight.Calcium in human body is distributed in bone and tooth mostly, and the calcium in bone and tooth accounts for about
The 99% of human calcium's total amount, remaining 1% is distributed in blood, intercellular fluid and soft tissue.For human body, muscle, nerve, body
In liquid and bone, the protein of all useful Ca combinations.Calcium is mankind's bone, the main inorganic composition of tooth and neurotransmission, flesh
The necessary elements such as meat contraction, blood clotting, hormone release and galactosis.Calcium all plays in each stage of growth in humans
Key effect, and calcium deficiency can cause skeleton lesion, seriously affect health.(1) children's calcium deficiency：It is not easy to fall asleep, be not easy
Into deep sleep, love is cryed, is easily waken up with a start, hidrosis after sleep after sleep；Paroxysmal abdominal pain, diarrhea knot, breastbone pain, " X "
Type leg, " O " type leg, pigeon breast, nail is greyish white or has white trace；Apocleisis, partial eclipse；Daytime irritability is on tenterhooks；Intellectual development is slow, says
Words evening；It in teething evening, just teethes after 10 months, teeth arrangement is sparse, irregular, not close, and tooth is darkly pointed or zigzag；Head
Send out sparse；Out of condition, easy to catch cold etc..(2) teenager's calcium deficiency：It can feel significantly to grow pain, leg is soft, knots；It is weary
Power, agitation, energy are not concentrated, and are easy tired；Calcium deficiency can reduce the elasticity and toughness of soft tissue.Skin, which lacks elasticity, seems slack and undisciplined,
Aging；Eye lens lack elasticity, easily myopia, presbyopic；Blood vessel lacks elasticity easily hardening；Partial eclipse, apocleisis；Decayed tooth, tooth development are not
It is good；Easy allergy, easy catching a cold etc..(3) between twenty and fifty calcium deficiency：Under normal circumstances, between twenty and fifty to have heavy life stress, nervous life
Movable joint, which is played, often makes them neglect some discomforts on body, and the age bracket calcium deficiency does not have typical symptom again in addition, so
It is easy to cover the state of an illness.When have regular burnout, it is weak, knot, ache all over, the symptoms such as easy allergy, easy catching a cold when, just answer
Suspect whether calcium deficiency.
The absorption of calcium is carried out in the form of organic chelated state, and organism is cannot directly to absorb inorganic calcium, because
This improves agricultural and sideline product calcium content, increases the intake of organic calcium, is the insufficient basic solution of human body calcium intake.
Invention content
In view of this, the purpose of the present invention is to provide a kind of rich calcium earthworm protein compounds and its preparation method and application
And by-product richness calcium wormcast.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical scheme：A kind of richness calcium earthworm protein compound, packet
Include rich calcium earthworm or rich calcium earthworm processing product；The calcium content of the richness calcium earthworm is 240~360mg/kg.
Preferably, the calcium content of the rich calcium earthworm is 280~300mg/kg.
The present invention also provides the preparation methods of the rich calcium earthworm protein compound, include the following steps：1) with cow dung,
Calcium source and stalk are that basic material piles up rich calcium vermiculture bed；The content of calcium constituent is 120 in the richness calcium vermiculture bed
~240mg/kg；2) earthworm kind is seeded in the rich calcium vermiculture bed, cultivates and obtains within 30~45 days rich calcium earthworm；3) will
The rich calcium earthworm protein compound of the broken acquisition of the richness calcium dry earthworm.
Preferably, the mass content of cow dung is 60~90% in the rich calcium vermiculture bed described in step 1).
Preferably, further include additional materials in the rich calcium vermiculture bed described in step 1), the additional materials be containing
The industrial or agricultural of organic nutrient substance or the material of grocery trade.
Preferably, the mass content of additional materials is 5~20% in the rich calcium vermiculture bed.
Preferably, further include vitamin D additive in rich calcium vermiculture bed described in step 1).
Preferably, the injected volume of earthworm kind described in step 2) is that every cubic metre of rich calcium vermiculture bed launches 1.5~1.8
Kilogram.
Application the present invention also provides the rich calcium earthworm protein compound as animal feed, food or health products.
The present invention also provides above-mentioned preparation methods to cultivate the rich calcium wormcast of rich calcium earthworm generation as organic fertilizer or poultry
The application of fowl feed, the richness calcium wormcast includes 60~100g/Kg of organic calcium；The total nitrogen content of the richness calcium wormcast is
1.2~4%；Content of tatal phosphorus is 2~4%；Full potassium content is 1.5~3.5%；The content of organic matter is 25~40%；Humus contains
Amount is 21~50%；Profitable strain content is 2.0 × 107~2.0 × 108/g；The pH value of the richness calcium wormcast is 6.8~
7.1。
Beneficial effects of the present invention：Richness calcium earthworm protein compound provided by the invention, calcium content are 240~360mg/
Kg, the richness calcium earthworm protein compound is on the basis of containing abundant earthworm protein, the also trace element containing high level
Calcium can be widely applied for rich calcium animal feed, food and health products, improve the added value of earthworm.
The cultural method of richness calcium earthworm provided by the invention, in the breeding process of earthworm by build rich calcium culturing bed come
Realize the cultivation of rich calcium earthworm, it is easy to operate, it is easy to spread；Earthworm grows in rich calcium culturing bed, has benefited from calcium to animal
Anti-oxidant equal health-care effects, earthworm bright color, the death rate are low.
The rich calcium wormcast nutrient that the rich calcium earthworm that the present invention obtains generates is comprehensive, is rich in organic matter, humic acid and micro- life
Object flora；Soil physics performance can be improved by being used as organic fertilizer, keep clay loose, sand is made to condense；It can promote soil air
Circulation accelerates microbial reproduction, is conducive to plant absorption nutrient；Water conservation, the nutrient preserving capability of soil can be enhanced.
Specific implementation mode
The present invention provides a kind of rich calcium earthworm protein compound, the calcium content of the richness calcium earthworm protein compound is
240~360mg/kg；Preferably 280~300mg/kg.The rich calcium earthworm protein compound described in the present invention is from richness
Calcium earthworm；The richness calcium earthworm is the fresh body of rich calcium earthworm or rich calcium earthworm stem body.The present invention is multiple to the rich calcium earthworm protein
The preparation method for closing object is not particularly limited, and adopts the product of drying and crushing richness calcium earthworm acquisition with the conventional methods in the field i.e.
It can；The richness calcium earthworm protein compound can be liquid or solid powder.
The present invention also provides the preparation methods of the rich calcium earthworm protein compound, include the following steps：1) with cow dung,
Calcium source and stalk are that basic material piles up rich calcium vermiculture bed；The content of calcium constituent is 120 in the richness calcium vermiculture bed
~240mg/kg；2) earthworm kind is seeded in the rich calcium vermiculture bed, cultivates and obtains within 30~45 days rich calcium earthworm；3) will
The rich calcium earthworm protein compound of the broken acquisition of the richness calcium dry earthworm.
In the present invention, rich calcium vermiculture bed is piled up for basic material with cow dung, calcium source and stalk and piles up rich calcium earthworm
Culturing bed.The present invention is not particularly limited the source of the cow dung, and the water content of the cow dung is preferably 60~70%, more
Preferably 62~68%；The present invention does not limit the type of the cow dung and source, the matter of cow dung in the culturing bed
Amount content is preferably 60~90%, more preferably 75~85%.
In the present invention, in the culturing bed calcium source content be 120~240mg/kg, preferably 160~220mg/kg,
More preferably 180~200mg/kg.In the present invention, the calcium source is preferably Ca nutrient.The present invention seeks the calcium
The source for supporting agent is not particularly limited, and specifically in implementation process of the present invention, the Ca nutrient source is sought for commercially available calcium
Support agent.
The present invention is not particularly limited the source of the stalk and type, using the stalk of this field routine.Tool
Body in implementation process of the present invention, the stalk can be maize straw, broomcorn straw, rice straw or wheat stalk.This
The mass content of stalk is preferably 5~20%, more preferably 10~15% in culturing bed described in invention.
Preferably further include in the present invention additional materials in the rich calcium vermiculture bed, the richness calcium vermiculture bed
The mass content of middle additional materials is preferably 5~20%.The additional materials preferably contain organic nutrition in the present invention
It may be raw material or product that the industrial or agricultural of substance or the material of grocery trade, which can be waste material,.Specifically implemented in the present invention
Journey include sludge, the residue of food factory, the offal of slaughterhouse, Milk Products Plant one or more of raffinate.In this hair
In bright, when the rich calcium earthworm is used as food or health products, the additional materials can be rice residue, pomace, vegetables slag etc., go back
Can be the food such as maize flour, carrot.
Preferably further include in the present invention vitamin D in the rich calcium vermiculture bed, the richness calcium vermiculture bed
The mass content of middle vitamin D is preferably 1~4%, more preferably 3%.Do not have to the source of the vitamin D in the present invention
There is restriction, using the high substance of the Vitamin D supplements or vitamin D content of this field routine.Heretofore described dimension
The effect of raw element D is the calcium uptake promoted during vermiculture.
In the present invention, rich the highly preferred of calcium vermiculture bed is 0.1~0.3m, more preferably 0.2m；Institute
The width for stating culturing bed is preferably 0.8~1.2m, more preferably 1.0m；The length of the culturing bed is preferably 20~
30m, more preferably 22~28m, most preferably 25m.The culturing bed advantageous cross sections are trapezoidal in the present invention
Strip ridge, bottom width is preferably 0.5~0.6m, lower bottom width is preferably 0.8~1.0m on trapezoid cross section.
In the present invention, several are preferably arranged in parallel in the culturing bed, and each two disposed in parallel is adjacent to support
Distance is preferably 2.5~3.5m, more preferably 3m between growing bed.It is preferable provided with draining between the adjacent culturing bed bed
The width of ditch, the gutter is preferably 0.3~0.6m, more preferably 0.5m.It is described in the present invention disposed in parallel
Several culturing bed both sides are preferable provided with walkway, and the width of the walkway is preferably 0.8~1.2m, more preferably
For 1.0m.
Due to earthworm non-refractory and low temperature, preferred above the culturing bed in the present invention to set up ceiling, summer hides
Positive sun-proof, Winter protection.The culturing bed center of top is preferable provided with aqueduct, is arranged on the aqueduct several
Hole for water spraying, the hole for water spraying are preferably evenly distributed on aqueduct both side surface, distance between the hole for water spraying is two neighboring
Preferably 0.1~0.3m, more preferably 0.2m；The hole for water spraying act as spraying water to culturing bed, keeps culturing bed
Humidity.
Earthworm kind is uniformly seeded in culturing bed and cultivates 30~45 days after obtaining rich calcium vermiculture bed by the present invention
Obtain rich calcium earthworm.The quality of the earthworm kind is 0.3~0.8g/ items in the present invention；The injected volume of the earthworm kind is preferred
It is every cubic metre and launches 1.5~1.8 kilograms, more preferably 1.6~1.7 kilograms every cubic metre；The dispensing of the earthworm kind
Quantity is preferably 3000~4000/cubic metre, more preferably 3200~3700/cubic metre.
In the present invention, the temperature of the vermiculture is preferably 15~25 DEG C, more preferably 20~24 DEG C, optimal
Choosing is 22 DEG C.The humidity of the vermiculture is preferably 60~80%, more preferably 65~75%, most preferably
70%.The culture-cycle brooding time is preferably 10~20 days.Time in growth period is preferably 20~25 days.
The present invention preferably should also carry out protection work during vermiculture, to prevent invading for mouse, frog, snake etc.
Evil.
The present invention is preferred to collect rich calcium earthworm after rich calcium vermiculture.The rich calcium earthworm described in the present invention
Collection method use this field routine earthworm collection method, preferably at the top of vermiculture bed laying earthworm cloth carry
Rich calcium earthworm is taken, earthworm is mobile to earthworm cloth lower section under illumination condition, illumination is repeated several times, to realize earthworm and wormcast
Separation.
Culturing bed is preferably reduced to original shape, supplement calcium battalion by the present invention after collecting rich calcium earthworm with cow dung
Agent is supported, next round cultivation is prepared.
The present invention determines the method suitably cultivated, the richness calcium in specific implementation process according to place and cultivation demand
Vermiculture bed is preferably outdoor/indoor culture, box/basket formula cultivation or three-dimensional culture.The rich calcium earthworm described in the present invention
Earthworm culturing bed can be indoor culture；Preferably the vermiculture bed is arranged on a moving belt when indoor culture, the transmission
Bandwidth is preferably 1~2m, long to be preferably 20~60m, and material thickness is preferably 10~30cm；The conveyer belt is preferably
Food-grade conveyer belt can facilitate the collection of earthworm and the separation of earthworm and wormcast using this cultural method, reduce artificial
Input.Heretofore described box/basket formula cultivation is using the case or basket of the vermiculture of this field routine, the richness calcium earthworm
Earthworm culturing bed sets length and width according to the size of case or basket, and thickness is preferably 10~30cm, more preferably 15cm.
The three-dimensional culture include by box/basket formula cultivation described in several case or basket solid place, or will be several outdoor
The culturing bed solid of cultivation is placed.The three-dimensional culture each layer of setting illumination in the breeding process, prevents earthworm from escaping.At this
Box described in invention/basket formula cultivation or three-dimensional culture can be outdoor outdoor cultivation, can also be indoor culture.
The present invention is after obtaining rich calcium earthworm, by the rich rich calcium earthworm protein compound of the broken acquisition of calcium dry earthworm.
The present invention method broken to the rich calcium dry earthworm is not particularly limited, and is adopted with the conventional methods in the field；It is described
Dry can be specifically freeze-drying, air-dried or natural drying.Heretofore described crushing uses the disintegrating apparatus of this field routine
, can be specifically pulverizer.Further include preferably enzymolysis after heretofore described crushing, heretofore described enzymolysis uses
The type and enzymolysis parameter of the enzyme of this field routine；The effect of heretofore described enzymolysis is by the rich calcium earthworm protein
Protein breakdown in compound is that molecular weight smaller is more easy to the albumen absorbed；Enzymolysis of the present invention does not change rich calcium earthworm egg
The existence form of calcium in white.
The rich calcium earthworm protein compound obtained the present invention also provides the cultural method as animal feed, food or
The application of health products, the calcium content in the richness calcium earthworm protein compound are 240~360mg/kg, preferably 260~
3200mg/kg, more preferably 280~300mg/kg.Richness calcium earthworm provided by the present invention is as animal feed in application, can
Being used alone also can be with other animal feed Application of composite.Richness calcium earthworm animal feed of the present invention is containing abundant earthworm
On the basis of albumen, also contain microelements of calcium, improves the added value of earthworm.
The rich calcium wormcast that the rich calcium earthworm obtained the present invention also provides the cultural method generates as organic fertilizer or
The application of poultry and livestock feed, the richness calcium wormcast includes 60~100g/Kg of organic calcium；The total nitrogen content of the richness calcium wormcast is
1.2~4%；Content of tatal phosphorus is 2~4%；Full potassium content is 1.5~3.5%；The content of organic matter is 25~40%；Humus contains
Amount is 21~50%；Profitable strain content is 2.0 × 107~2.0 × 108/g；The pH value of the richness calcium wormcast is 6.8~
7.1.The organic fertilizer nutrient of heretofore described richness calcium wormcast is comprehensive, is rich in organic matter, humic acid and microorganism species.It can improve
Soil physics performance keeps clay loose, sand is made to condense；Soil air can be promoted to circulate, accelerate microbial reproduction, be conducive to plant
Object absorbs nutrient；Water conservation, nutrient preserving capability can be enhanced；Energy sorption based compositions play exchange interaction, prevent the use of excessive chemical fertilizer from bringing
Harm；The minerals in soil can be decomposed, are utilized for plant；It is used in mixed way with other chemical fertilizer, fertilizer efficiency is permanent；To planting
Object, people, animal are harmless, can also enhance resistance of the plant to pest and disease damage, inhibit plant soil-borne diseases, improve crop quality, extensive
Multiple crop natural flavor.The richness calcium wormcast organic fertilizer is with the following functions：Significantly improve the organic calcium content of crops；Promote
Crop growth increases yield；Degradation heavy metal (lead, cadmium, mercury, arsenic, chromium)；Enhance cropper resistance, reduces corps diseases.
In the present invention, the rich calcium wormcast includes 60~100g/Kg of organic calcium；The full nitrogen of the richness calcium wormcast contains
Amount is 1.2~4%；Content of tatal phosphorus is 2~4%；Full potassium content is 1.5~3.5%；The content of organic matter is 25~40%；Corruption is planted
Matter content is 21~50%；Profitable strain content is 2.0 × 107~2.0 × 108/g；The pH value of the richness calcium wormcast is
6.8~7.1.
In the present invention, the rich calcium wormcast includes following nutrition (air-dried)：Adsorb water 4.60~5.2%；Slightly
Fat 0.59~0.65%；Crude fibre 5.10~6.2%；Crude protein 5.10~21.7%；Coarse ash 70.8~73.55%；Nothing
Nitrogen extract 12.15~13.95%；Calcium 3.60~4.2% and phosphorus 0.30~0.4%.The spy of heretofore described wormcast
Point is that cinder content is high, and crude protein content is far above gramineous straw, and near or above pulse family stalk, it is the good feeding of livestock and poultry
One of material.
In the present invention, the rich calcium wormcast includes following mineral trace element content (μ g/g)：Iron (Fe) 3100
~3200；Manganese (Mn) 210~250；Zinc (Zn) 2.5~3.1；Copper (Cu) 20.1~21.1；Magnesium (Mg) 8400~8530.
In the present invention, the rich calcium wormcast includes 6~18 kinds of amino acid 1, and amino acid content (%) is such as in air dry matter
Under：Asparatate 0.50~0.60, threonine 0.20~0.35, serine 0.30~0.51, glutamic acid 0.40~0.65 are sweet
Propylhomoserin 0.10~0.20, alanine 0.35~0.75, valine 0.30~0.40, methionine 0.10~0.15, isoleucine
0.15~0.18, leucine 0.25~0.33, tyrosine 0.10~0.15, phenylalanine 0.20~0.25, lysine 0.18~
0.23, histidine 0.00~0.10, proline 0.16~0.19, cystine 0.00~0.12, tryptophan 0.20~0.23, smart ammonia
Acid 0.11~0.16.
In the present invention, the rich calcium wormcast beneficial microbe content is between/gram of 20,000,000/gram~200,000,000.Through
It detects, contains at least two plants of antagonistic microbes (styreptomyces globispotus strain and syringin streptomycete) in wormcast under normal circumstances, to soil
Fax bacterium plant disease has certain control action.
It is method of administration, amount of application or feeding method, feeding when heretofore described richness calcium wormcast is as fertilizer or feed
Amount refers to common fertilizer or feed.
With reference to embodiment to rich calcium earthworm protein compound provided by the invention and its preparation method and application and
By-product richness calcium wormcast is described in detail, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
Selection is close to water source, draining is convenient, ventilation, the spacious location far from urban district is with cow dung and each breeding stock of Ca nutrient
Poultry manure 60% is sought with stalk, sludge, the residue of food factory, the offal of slaughterhouse, the raffinate etc. 40% of Milk Products Plant and calcium
Foster agent is prepared into rich calcium vermiculture bed, and the content of calcium is 160mg/kg in culturing bed, and earthworm bed is that cross section is trapezoidal length
Ridge, high 0.2m, long 25m, bottom width 0.5m, lower bottom width 1m on trapezoid cross section, distance 3m between adjacent earthworm bed, adjacent earthworm
The gutter of width 0.5m can be equipped between bed, earthworm bed both sides set width as the walkway of 1m, then set up and hide above earthworm bed
Yang Peng, earthworm bed center of top laying water pipes, opens up hole for water spraying, hole for water spraying is evenly distributed on water-supply-pipe on aqueduct
Road both side surface, hole for water spraying distance 0.2m between any two；Earthworm kind to be launched into earthworm bed, earthworm kind injected volume is 1.5kg/ ㎡,
Hole for water spraying is periodically sprayed water, and it is 80% to keep earthworm bed humidity；It periodically inspects, pays attention to earthworm growth, breeding and oviposition, night lights
Illumination, prevents earthworm from fleeing；By raising in 45 days, earthworm grew up to, and earthworm cloth is laid at the top of earthworm bed and is extracted into earthworm, is gone out after earthworm again
Earthworm bed is reduced to original trapezoidal strip ridge with cow dung, thus the rich calcium earthworm of cultural method production, using inductive coupling etc.
Ionomer emission spectrum method measures calcium content, and calcium content fresh weight reaches 240mg/kg (fresh weight).
Embodiment 2
It selects close to water source, draining is convenient, divulge information, the spacious location in separate urban district prepares rich calcium with cow dung and Ca nutrient
Vermiculture bed, the content of calcium is 280mg/kg in culturing bed, and earthworm bed is that cross section is trapezoidal strip ridge, and high 0.2m is long
25m, bottom width 0.5m, lower bottom width 1m on trapezoid cross section, distance 3m between adjacent earthworm bed can be equipped with width between adjacent earthworm bed
The gutter of 0.5m, earthworm bed both sides set width as the walkway of 1m, and sunshade, earthworm bed top are then set up above earthworm bed
Central laying water pipes open up hole for water spraying on aqueduct, and hole for water spraying is evenly distributed on aqueduct both side surface, water spray
Hole distance 0.2m between any two；Earthworm kind to be launched into earthworm bed, earthworm kind injected volume is 1.5kg/ ㎡, and hole for water spraying is periodically sprayed water,
It is 70% to keep earthworm bed humidity；It periodically inspects, notices that earthworm growth, breeding and oviposition, night lights illumination prevent outside earthworm
Escape；By raising in 45 days, earthworm grew up to, and earthworm cloth is laid at the top of earthworm bed and is extracted into earthworm, go out after earthworm again with cow dung by earthworm bed also
Originally it was original trapezoidal strip ridge, the thus rich calcium earthworm of cultural method production, using inductively coupled plasma atomic emission
Method measures calcium content, and calcium content fresh weight reaches 280mg/kg (fresh weight).
Embodiment 3
It selects close to water source, draining is convenient, divulge information, the spacious location in separate urban district prepares rich calcium with cow dung and Ca nutrient
Vermiculture bed, the content of calcium is 170mg/kg in culturing bed, and earthworm bed is that cross section is trapezoidal strip ridge, and high 0.2m is long
25m, bottom width 0.5m, lower bottom width 1m on trapezoid cross section, distance 3m between adjacent earthworm bed can be equipped with width between adjacent earthworm bed
The gutter of 0.5m, earthworm bed both sides set width as the walkway of 1m, and sunshade, earthworm bed top are then set up above earthworm bed
Central laying water pipes open up hole for water spraying on aqueduct, and hole for water spraying is evenly distributed on aqueduct both side surface, water spray
Hole distance 0.2m between any two；Earthworm kind to be launched into earthworm bed, earthworm kind injected volume is 1.5kg/ ㎡, and hole for water spraying is periodically sprayed water,
It is 60% to keep earthworm bed humidity；It periodically inspects, notices that earthworm growth, breeding and oviposition, night lights illumination prevent outside earthworm
Escape；By raising in 45 days, earthworm grew up to, and earthworm cloth is laid at the top of earthworm bed and is extracted into earthworm, go out after earthworm again with cow dung by earthworm bed also
Originally it was original trapezoidal strip ridge, the thus rich calcium earthworm of cultural method production, using inductively coupled plasma atomic emission
Method measures calcium content, and calcium content fresh weight reaches 280mg/kg (fresh weight).
Embodiment 4
Selection is close to water source, draining is convenient, ventilation, the spacious location far from urban district is with cow dung and Ca nutrient and vitamin
D prepares rich calcium vermiculture bed, and the content of calcium is 170mg/kg in culturing bed, and earthworm bed is that cross section is trapezoidal strip ridge,
High 0.2m, long 25m, bottom width 0.5m, lower bottom width 1m on trapezoid cross section, distance 3m between adjacent earthworm bed, adjacent earthworm bed
Between can be equipped with the gutter of width 0.5m, earthworm bed both sides set width as the walkway of 1m, then set up sunshade above earthworm bed,
Earthworm bed center of top laying water pipes, open up hole for water spraying, hole for water spraying is evenly distributed on aqueduct two on aqueduct
Side surface, hole for water spraying distance 0.2m between any two；Earthworm kind is launched into earthworm bed, earthworm kind injected volume is 1.5kg/ ㎡, water spray
Hole is periodically sprayed water, and it is 60% to keep earthworm bed humidity；It periodically inspects, notices that earthworm growth, breeding and oviposition, night lights are shone
It is bright, prevent earthworm from fleeing；By raising in 45 days, earthworm grew up to, and earthworm cloth is laid at the top of earthworm bed and is extracted into earthworm, is used again after going out earthworm
Earthworm bed is reduced to original trapezoidal strip ridge by cow dung, thus cultural method production rich calcium earthworm, using inductive coupling etc. from
Daughter emission spectrographic determination calcium content, calcium content fresh weight reach 280mg/kg, and vitamin D content reaches 93mg/kg (fresh weight).
Embodiment 5
Cow dung and Ca nutrient and stalk is used to prepare rich calcium vermiculture bed indoors, the calcium vermiculture bed setting exists
On conveyer belt, the conveyer belt is food-grade conveyer belt, and the transmission bandwidth 1.5m, long 40m, material thickness is 15cm；Use this
Cultural method can facilitate the collection of earthworm and the separation of earthworm and wormcast, reduce artificial input.Each culturing bed
The content of middle calcium is 0.08mg/kg, and earthworm kind is launched into culturing bed, and earthworm kind injected volume is 1.6kg/ ㎡, keeps earthworm bed temperature
22 DEG C of degree, humidity 60%；It periodically inspects, pays attention to earthworm growth, breeding and oviposition, lighting is provided, prevents earthworm from fleeing；
By raising in 45 days, earthworm grew up to, and collected into earthworm, thus the rich calcium earthworm of cultural method production, using inductive coupling etc. from
Daughter emission spectrographic determination calcium content, calcium content fresh weight reach 280mg/kg (fresh weight).
Embodiment 6
With cow dung and Ca nutrient, vitamin D and stalk, (cow dung accounts for 50%, and stalk accounts for 42%, and Ca nutrient accounts for indoors
5%, vitamin D accounts for and 3%) prepares rich calcium vermiculture bed, and the calcium vermiculture bed is arranged on a moving belt, the conveyer belt
For food-grade conveyer belt, the transmission bandwidth 1.5m, long 40m, material thickness is 15cm；It can facilitate earthworm using this cultural method
Collection and earthworm and wormcast separation, reduce artificial input.The content of calcium is 0.08mg/ in each culturing bed
Kg launches earthworm kind into culturing bed, and earthworm kind injected volume is 1.6kg/ ㎡, keeps 22 DEG C of earthworm bed tempertaure, humidity 60%；It is fixed
Phase inspects, and pays attention to earthworm growth, breeding and oviposition, provides lighting, prevent earthworm from fleeing；By raising in 45 days, earthworm
Grow up to, collects into earthworm, thus the rich calcium earthworm of cultural method production, calcium is measured using inductively coupled plasma emission spectrography
Content, calcium content fresh weight reach 280mg/kg, and vitamin D content reaches 93mg/kg (fresh weight).
Embodiment 7
Box cultivation is used indoors, and with cow dung and Ca nutrient, stalk, vegetables slag, (cow dung accounts for 50% stalk and vegetables
Slag accounts for 45%, and Ca nutrient accounts for and 5%) prepares rich calcium vermiculture bed, and earthworm bedside 1.6m, material thickness is 20cm, is put in case；
The content of calcium is 140mg/kg in each box culturing bed, earthworm kind is launched into culturing bed, earthworm kind injected volume is
1.7kg/ ㎡ keep 22 DEG C of earthworm bed tempertaure, humidity 65%；It periodically inspects, pays attention to earthworm growth, breeding and oviposition, provide
Lighting prevents earthworm from fleeing；By raising in 45 days, earthworm grew up to, and collected into earthworm, the rich calcium that thus cultural method produces
Earthworm measures calcium content using inductively coupled plasma emission spectrography, and calcium content fresh weight reaches 300mg/kg (fresh weight).
Embodiment 8
It uses basket formula to cultivate indoors, rich calcium vermiculture bed, earthworm is prepared with cow dung and Ca nutrient, stalk, rice residue
Bedside 1.8m, material thickness are 25cm, are put in basket；The content of calcium is 280mg/kg in each basket of formula culturing bed, by earthworm kind
It launches in culturing bed, earthworm kind injected volume is 1.7kg/ ㎡, keeps 22 DEG C of earthworm bed tempertaure, humidity 70%；It periodically inspects, notes
Earthworm of anticipating grows, breeds and lays eggs, and provides lighting, prevents earthworm from fleeing；By raising in 45 days, earthworm grew up to, and collected
At earthworm, thus the rich calcium earthworm of cultural method production, measures calcium content, calcium contains using inductively coupled plasma emission spectrography
Amount fresh weight reaches 200mg/kg (fresh weight).
Embodiment 9
Three-dimensional culture is used indoors, and with cow dung and Ca nutrient, stalk, pomace, (cow dung accounts for 50% stalk and pomace accounts for
45%, Ca nutrient accounts for and 5%) prepares rich calcium vermiculture bed, and earthworm bedside 1m, material thickness is 25cm, is put in basket；It is described every
The content of calcium is 170mg/kg in a basket of formula culturing bed, and earthworm kind is launched into culturing bed, and earthworm kind injected volume is 1.5kg/ ㎡, is protected
Hold 23 DEG C of earthworm bed tempertaure, humidity 75%；It periodically inspects, pays attention to earthworm growth, breeding and oviposition, lighting is provided, prevent
Only earthworm is fled；By raising in 45 days, earthworm grew up to, and collected into earthworm, the rich calcium earthworm that thus cultural method produces, using electricity
Feel coupled plasma optical emission spectrographic determination calcium content, calcium content fresh weight reaches 200mg/kg (fresh weight).
Embodiment 10
Use three-dimensional culture indoors, with cow dung and Ca nutrient, vitamin D, stalk, pomace (cow dung accounts for 50% stalk,
Pomace accounts for 42%, and Ca nutrient accounts for 5%, and vitamin D accounts for and 3%) prepares rich calcium vermiculture bed, earthworm bedside 1m, and material thickness is
25cm is put in basket；The content of calcium is 170mg/kg in each basket of formula culturing bed, earthworm kind is launched into culturing bed, earthworm
Kind injected volume is 1.5kg/ ㎡, keeps 23 DEG C of earthworm bed tempertaure, humidity 75%；Periodically inspect, pay attention to earthworm growth, breeding and
Oviposition, provides lighting, prevents earthworm from fleeing；By raising in 45 days, earthworm grew up to, and collected into earthworm, thus cultural method
The rich calcium earthworm of production measures calcium content using inductively coupled plasma emission spectrography, and calcium content fresh weight reaches 200mg/
Kg, vitamin D content reach 67mg/kg (fresh weight).
Embodiment 11
The rich calcium wormcast that rich calcium earthworm described in embodiment 1 generates, can be used as organic fertilizer application.The richness calcium earthworm
Excrement includes organic calcium 80g/Kg；The total nitrogen content of the richness calcium wormcast is 1.5%；Content of tatal phosphorus is 1.9%；Potassium content is entirely
2.0%；The content of organic matter is 35%；Humus content is 38%；Profitable strain content is 2.0 × 107/g；The richness calcium earthworm
The pH value of earthworm excrement is 6.8.
In the present invention, the wormcast includes following nutrition (air-dried)：Adsorb water 4.60%；Crude fat
0.59%；Crude fibre 5.10%；Crude protein 5.10%；Coarse ash 70.8%；Nitrogen-free extracts 12.15%；Calcium 3.60% and phosphorus
0.30%.The characteristics of heretofore described wormcast is that cinder content is high, and crude protein content is far above gramineous straw, and connects
Closely or it is more than pulse family stalk, is one of good feed of livestock and poultry.
In the present invention, the wormcast includes following mineral trace element content (μ g/g)：Iron (Fe) 3100；Manganese
(Mn)210；Zinc (Zn) 2.5；Copper (Cu) 20.1；Magnesium (Mg) 8400.
In the present invention, the wormcast includes 6~18 kinds of amino acid 1, and amino acid content (%) is as follows in air dry matter：It
L-aminobutanedioic acid 0.50, threonine 0.20, serine 0.30, glutamic acid 0.40, glycine 0.10, alanine 0.35, valine
0.30, methionine 0.10, isoleucine 0.1, leucine 0.25, tyrosine 0.10, phenylalanine 0.20, lysine 0.18, group
Propylhomoserin 0.10, proline 0.16, cystine 0.12, tryptophan 0.23, arginine 0.11.
In the present invention, the wormcast wormcast beneficial microbe content is in 20,000,000/gram.After testing, wormcast
In under normal circumstances contain at least two plants of antagonistic microbes (styreptomyces globispotus strain and syringin streptomycete), to soil-borne fungus phytopathy
Evil has certain control action.
Embodiment 12
The rich calcium wormcast that rich calcium earthworm described in embodiment 2 generates, can be used as organic fertilizer application.The richness calcium earthworm
Excrement includes organic calcium 30g/Kg；The total nitrogen content of the richness calcium wormcast is 2.2%；Content of tatal phosphorus is 1.5%；Potassium content is entirely
1.3%；The content of organic matter is 25%；Humus content is 26%；Profitable strain content is 2.0 × 108/g；The richness calcium earthworm
The pH value of earthworm excrement is 7.0.
In the present invention, the wormcast includes following nutrition (air-dried)：Adsorb water 5.2%；Crude fat 0.60%；
Crude fibre 5.70%；Crude protein 9.2%；Coarse ash 71.2%；Nitrogen-free extracts 12.51%；Calcium 3.80% and phosphorus 0.32%.This
The characteristics of wormcast described in invention is that cinder content is high, and crude protein content is far above gramineous straw, and near or above
Pulse family stalk is one of good feed of livestock and poultry.
In the present invention, the wormcast includes following mineral trace element content (μ g/g)：Iron (Fe) 3150；Manganese
(Mn)240；Zinc (Zn) 2.9；Copper (Cu) 20.3；Magnesium (Mg) 8480.
In the present invention, the wormcast includes 8 kinds of amino acid 1, and amino acid content (%) is as follows in air dry matter：Lucid asparagus
Propylhomoserin 0.55, threonine 0.34, serine 0.43, glutamic acid 0.56, glycine 0.18, alanine 0.65, valine 0.37, egg
Propylhomoserin 0.13, isoleucine 0.1, leucine 0.25, tyrosine 0.10, phenylalanine 0.20, lysine 0.18, histidine
0.10, proline 0.16, cystine 0.12, tryptophan 0.23, arginine 0.11.
In the present invention, the wormcast beneficial microbe content is in 1.5 hundred million/gram.After testing, positive reason in wormcast
Contain at least two plants of antagonistic microbes (styreptomyces globispotus strain and syringin streptomycete) under condition, has centainly to soil-borne fungus plant disease
Control action.
By above-described embodiment it is found that richness calcium earthworm provided by the present invention contains abundant animal protein and trace element
Calcium can be widely applied for rich calcium animal feed, improve the added value of earthworm；By building rich calcium cultivation in the breeding process
Bed realizes the cultivation of rich calcium earthworm, easy to operate, easy to spread；The earthworm bright color of cultivation, the death rate are low.Institute of the present invention
The rich calcium wormcast nutrient stated is comprehensive, is rich in organic matter, humic acid and microorganism species；Soil physics performance can be improved, enhanced
The water conservation of soil, nutrient preserving capability.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of richness calcium earthworm protein compound, which is characterized in that the calcium content of the richness calcium earthworm protein compound is 240~
360mg/kg；Calcium in the richness calcium earthworm protein compound exists in the form of calcium compound protein.
2. richness calcium earthworm protein compound according to claim 1, which is characterized in that the richness calcium earthworm protein compound
Calcium content be 280~300mg/kg.
3. the preparation method of rich calcium earthworm protein compound, includes the following steps described in claims 1 or 2：
1) it is that basic material piles up rich calcium vermiculture bed with cow dung, calcium source and stalk；Calcium member in the richness calcium vermiculture bed
The content of element is 120~240mg/kg；
2) earthworm kind is seeded in the rich calcium vermiculture bed, cultivates and obtains within 30~45 days rich calcium earthworm；
3) by the rich rich calcium earthworm protein compound of the broken acquisition of calcium dry earthworm.
4. the preparation method of rich calcium earthworm protein compound according to claim 3, which is characterized in that described in step 1)
The mass content of cow dung is 60~90% in rich calcium vermiculture bed.
5. the preparation method of rich calcium earthworm protein compound according to claim 3, which is characterized in that described in step 1)
Further include additional materials in rich calcium vermiculture bed, the additional materials are industrial or agricultural or grocery trade containing organic nutrient substance
Material.
6. the preparation method of rich calcium earthworm protein compound according to claim 5, which is characterized in that the richness calcium earthworm is supported
The mass content for growing additional materials in bed is 5~20%.
7. according to the preparation method of the rich calcium earthworm protein compound of claim 3 or 5, which is characterized in that institute in step 1)
It further includes vitamin D additive to state in rich calcium vermiculture bed.
8. the preparation method of rich calcium earthworm protein compound according to claim 3, which is characterized in that earthworm described in step 2)
The injected volume of earthworm kind is that every cubic metre of rich calcium vermiculture bed launches 1.5~1.8 kilograms.
9. preparation method obtains described in rich calcium earthworm protein compound or claim 3~8 any one described in claims 1 or 2
Application of the rich calcium earthworm protein compound arrived as animal feed, food or health products.
10. cultivating the rich calcium wormcast of rich calcium earthworm generation in preparation method described in claim 3~8 any one as organic
The application of fertilizer or animal and fowl fodder, which is characterized in that the richness calcium wormcast includes 60~100g/Kg of organic calcium；The richness calcium earthworm
The total nitrogen content of earthworm excrement is 1.2~4%；Content of tatal phosphorus is 2~4%；Full potassium content is 1.5~3.5%；The content of organic matter is 25
~40%；Humus content is 21~50%；Profitable strain content is 2.0 × 107~2.0 × 108A/g；The richness calcium earthworm
The pH value of excrement is 6.8~7.1.
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