CN108374039A - 一种人乳头瘤病毒的快速检测方法、液相芯片及试剂盒 - Google Patents
一种人乳头瘤病毒的快速检测方法、液相芯片及试剂盒 Download PDFInfo
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- CN108374039A CN108374039A CN201810102320.6A CN201810102320A CN108374039A CN 108374039 A CN108374039 A CN 108374039A CN 201810102320 A CN201810102320 A CN 201810102320A CN 108374039 A CN108374039 A CN 108374039A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/708—Specific hybridization probes for papilloma
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
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CN201810102320.6A CN108374039B (zh) | 2018-02-01 | 2018-02-01 | 一种人乳头瘤病毒的快速检测方法、液相芯片及试剂盒 |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109112176A (zh) * | 2018-09-04 | 2019-01-01 | 武汉尚码生物科技有限公司 | 一种微球编码载体及其应用 |
CN111948185A (zh) * | 2020-08-21 | 2020-11-17 | 中国药科大学 | 基于二维扩增阵列模式的瞬时识别急性肾损伤发展进程的传感器、制备方法及用途和用法 |
CN112195281A (zh) * | 2020-11-12 | 2021-01-08 | 武汉凯德维斯生物技术有限公司 | 一种用于检测人乳头瘤病毒hpv56的探针及其试剂盒 |
CN112920267A (zh) * | 2021-03-09 | 2021-06-08 | 北京康乐卫士生物技术股份有限公司 | 一种抗人乳头瘤病毒31型的单克隆中和抗体及其应用 |
WO2022201007A1 (es) * | 2021-03-24 | 2022-09-29 | Universidad El Bosque | Biosensor para la detección óptica de la infección por el virus del papiloma humano en fluidos biológicos |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20040083674A (ko) * | 2003-03-24 | 2004-10-06 | 바이오코아 주식회사 | 인유두종바이러스(hpv)유전자형 분석용 서스펜션어레이 마이크로스피어 비드 조성물 및 그 검사 방법 |
CN1814796A (zh) * | 2005-12-02 | 2006-08-09 | 浙江大学 | 一种用于26种人乳头瘤病毒的检测与分型的方法 |
CN1948503A (zh) * | 2005-10-10 | 2007-04-18 | 上海透景生命科技有限公司 | 一种人乳头瘤病毒检测分型方法及试剂盒 |
WO2008074182A1 (fr) * | 2006-12-18 | 2008-06-26 | Shanghai Tellgen Life Science Co., Ltd. | Procédé de détection et de typage du virus du papillome humain et kit de réactifs dudit procédé |
CN101760565A (zh) * | 2008-11-21 | 2010-06-30 | 叶军 | 一种悬浮芯片技术检测hpv方法 |
-
2018
- 2018-02-01 CN CN201810102320.6A patent/CN108374039B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20040083674A (ko) * | 2003-03-24 | 2004-10-06 | 바이오코아 주식회사 | 인유두종바이러스(hpv)유전자형 분석용 서스펜션어레이 마이크로스피어 비드 조성물 및 그 검사 방법 |
CN1948503A (zh) * | 2005-10-10 | 2007-04-18 | 上海透景生命科技有限公司 | 一种人乳头瘤病毒检测分型方法及试剂盒 |
CN1814796A (zh) * | 2005-12-02 | 2006-08-09 | 浙江大学 | 一种用于26种人乳头瘤病毒的检测与分型的方法 |
WO2008074182A1 (fr) * | 2006-12-18 | 2008-06-26 | Shanghai Tellgen Life Science Co., Ltd. | Procédé de détection et de typage du virus du papillome humain et kit de réactifs dudit procédé |
CN101760565A (zh) * | 2008-11-21 | 2010-06-30 | 叶军 | 一种悬浮芯片技术检测hpv方法 |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109112176A (zh) * | 2018-09-04 | 2019-01-01 | 武汉尚码生物科技有限公司 | 一种微球编码载体及其应用 |
CN111948185A (zh) * | 2020-08-21 | 2020-11-17 | 中国药科大学 | 基于二维扩增阵列模式的瞬时识别急性肾损伤发展进程的传感器、制备方法及用途和用法 |
CN112195281A (zh) * | 2020-11-12 | 2021-01-08 | 武汉凯德维斯生物技术有限公司 | 一种用于检测人乳头瘤病毒hpv56的探针及其试剂盒 |
CN112920267A (zh) * | 2021-03-09 | 2021-06-08 | 北京康乐卫士生物技术股份有限公司 | 一种抗人乳头瘤病毒31型的单克隆中和抗体及其应用 |
WO2022201007A1 (es) * | 2021-03-24 | 2022-09-29 | Universidad El Bosque | Biosensor para la detección óptica de la infección por el virus del papiloma humano en fluidos biológicos |
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