CN108267581A - 用于高通量多重检测的系统、装置和方法 - Google Patents
用于高通量多重检测的系统、装置和方法 Download PDFInfo
- Publication number
- CN108267581A CN108267581A CN201810002554.3A CN201810002554A CN108267581A CN 108267581 A CN108267581 A CN 108267581A CN 201810002554 A CN201810002554 A CN 201810002554A CN 108267581 A CN108267581 A CN 108267581A
- Authority
- CN
- China
- Prior art keywords
- capture agent
- array
- capture
- cell
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 136
- 238000001514 detection method Methods 0.000 title claims abstract description 117
- 150000001875 compounds Chemical class 0.000 claims abstract description 210
- 239000003153 chemical reaction reagent Substances 0.000 claims description 355
- 239000003795 chemical substances by application Substances 0.000 claims description 77
- 230000003595 spectral effect Effects 0.000 claims description 28
- 238000000926 separation method Methods 0.000 claims description 26
- 239000012530 fluid Substances 0.000 claims description 23
- -1 Microwell array Chemical class 0.000 claims description 12
- 239000000126 substance Substances 0.000 claims description 8
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 238000004458 analytical method Methods 0.000 abstract description 71
- 238000001228 spectrum Methods 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 504
- 102000004169 proteins and genes Human genes 0.000 description 196
- 108090000623 proteins and genes Proteins 0.000 description 196
- 230000028327 secretion Effects 0.000 description 108
- 238000002474 experimental method Methods 0.000 description 58
- 102000004127 Cytokines Human genes 0.000 description 49
- 108090000695 Cytokines Proteins 0.000 description 49
- 239000004205 dimethyl polysiloxane Substances 0.000 description 47
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 47
- 206010028980 Neoplasm Diseases 0.000 description 42
- 108090000765 processed proteins & peptides Proteins 0.000 description 40
- 238000003556 assay Methods 0.000 description 38
- 239000011521 glass Substances 0.000 description 34
- 238000003491 array Methods 0.000 description 33
- 108090001007 Interleukin-8 Proteins 0.000 description 32
- 102000004890 Interleukin-8 Human genes 0.000 description 32
- 239000002158 endotoxin Substances 0.000 description 32
- 229920006008 lipopolysaccharide Polymers 0.000 description 32
- 239000000243 solution Substances 0.000 description 31
- 150000007523 nucleic acids Chemical class 0.000 description 30
- 102000004196 processed proteins & peptides Human genes 0.000 description 29
- 102100021943 C-C motif chemokine 2 Human genes 0.000 description 27
- 101710155857 C-C motif chemokine 2 Proteins 0.000 description 27
- 210000002540 macrophage Anatomy 0.000 description 27
- 102000039446 nucleic acids Human genes 0.000 description 26
- 201000011510 cancer Diseases 0.000 description 22
- 108020004707 nucleic acids Proteins 0.000 description 22
- 230000000638 stimulation Effects 0.000 description 21
- 238000000059 patterning Methods 0.000 description 20
- 238000002965 ELISA Methods 0.000 description 19
- 230000001413 cellular effect Effects 0.000 description 19
- 210000002865 immune cell Anatomy 0.000 description 19
- 210000001519 tissue Anatomy 0.000 description 19
- 230000027455 binding Effects 0.000 description 18
- 230000000875 corresponding effect Effects 0.000 description 18
- 230000006870 function Effects 0.000 description 18
- 238000002493 microarray Methods 0.000 description 18
- 108090001005 Interleukin-6 Proteins 0.000 description 17
- 102000004889 Interleukin-6 Human genes 0.000 description 17
- 239000000758 substrate Substances 0.000 description 16
- 239000003102 growth factor Substances 0.000 description 15
- 239000000427 antigen Substances 0.000 description 14
- 108091007433 antigens Proteins 0.000 description 14
- 102000036639 antigens Human genes 0.000 description 14
- 239000002773 nucleotide Substances 0.000 description 14
- 125000003729 nucleotide group Chemical group 0.000 description 14
- 239000000523 sample Substances 0.000 description 14
- 239000006285 cell suspension Substances 0.000 description 13
- 230000004044 response Effects 0.000 description 12
- 241000894007 species Species 0.000 description 12
- 238000003384 imaging method Methods 0.000 description 11
- 238000011534 incubation Methods 0.000 description 11
- 230000003834 intracellular effect Effects 0.000 description 11
- 229920000729 poly(L-lysine) polymer Polymers 0.000 description 11
- 230000003248 secreting effect Effects 0.000 description 11
- 238000012360 testing method Methods 0.000 description 11
- 238000011068 loading method Methods 0.000 description 10
- 108010027322 single cell proteins Proteins 0.000 description 10
- 102000001327 Chemokine CCL5 Human genes 0.000 description 9
- 108010055166 Chemokine CCL5 Proteins 0.000 description 9
- 102000007079 Peptide Fragments Human genes 0.000 description 9
- 108010033276 Peptide Fragments Proteins 0.000 description 9
- 210000001744 T-lymphocyte Anatomy 0.000 description 9
- 238000004113 cell culture Methods 0.000 description 9
- 230000002596 correlated effect Effects 0.000 description 9
- 239000012634 fragment Substances 0.000 description 9
- 238000007639 printing Methods 0.000 description 9
- 238000011002 quantification Methods 0.000 description 9
- 238000010186 staining Methods 0.000 description 9
- 102000009024 Epidermal Growth Factor Human genes 0.000 description 8
- 102000034655 MIF Human genes 0.000 description 8
- 108060004872 MIF Proteins 0.000 description 8
- 108010090804 Streptavidin Proteins 0.000 description 8
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 8
- 230000003915 cell function Effects 0.000 description 8
- IJOOHPMOJXWVHK-UHFFFAOYSA-N chlorotrimethylsilane Chemical compound C[Si](C)(C)Cl IJOOHPMOJXWVHK-UHFFFAOYSA-N 0.000 description 8
- 238000010586 diagram Methods 0.000 description 8
- 239000012636 effector Substances 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 8
- 239000012091 fetal bovine serum Substances 0.000 description 8
- PHEDXBVPIONUQT-RGYGYFBISA-N phorbol 13-acetate 12-myristate Chemical compound C([C@]1(O)C(=O)C(C)=C[C@H]1[C@@]1(O)[C@H](C)[C@H]2OC(=O)CCCCCCCCCCCCC)C(CO)=C[C@H]1[C@H]1[C@]2(OC(C)=O)C1(C)C PHEDXBVPIONUQT-RGYGYFBISA-N 0.000 description 8
- 102100040247 Tumor necrosis factor Human genes 0.000 description 7
- 238000004422 calculation algorithm Methods 0.000 description 7
- 239000006143 cell culture medium Substances 0.000 description 7
- 230000009260 cross reactivity Effects 0.000 description 7
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 238000009826 distribution Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 238000000684 flow cytometry Methods 0.000 description 7
- 229940088597 hormone Drugs 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 230000005012 migration Effects 0.000 description 7
- 238000013508 migration Methods 0.000 description 7
- 210000004980 monocyte derived macrophage Anatomy 0.000 description 7
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 6
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 6
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 6
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 6
- 125000003275 alpha amino acid group Chemical group 0.000 description 6
- 125000000539 amino acid group Chemical group 0.000 description 6
- 238000000708 deep reactive-ion etching Methods 0.000 description 6
- 238000011156 evaluation Methods 0.000 description 6
- 208000005017 glioblastoma Diseases 0.000 description 6
- 230000005484 gravity Effects 0.000 description 6
- 239000005556 hormone Substances 0.000 description 6
- 238000010191 image analysis Methods 0.000 description 6
- 238000003018 immunoassay Methods 0.000 description 6
- 230000002757 inflammatory effect Effects 0.000 description 6
- 239000010410 layer Substances 0.000 description 6
- 239000003446 ligand Substances 0.000 description 6
- 229910052710 silicon Inorganic materials 0.000 description 6
- 239000010703 silicon Substances 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 210000004881 tumor cell Anatomy 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 5
- 102000019034 Chemokines Human genes 0.000 description 5
- 108010012236 Chemokines Proteins 0.000 description 5
- 101150021185 FGF gene Proteins 0.000 description 5
- 102000002689 Toll-like receptor Human genes 0.000 description 5
- 108020000411 Toll-like receptor Proteins 0.000 description 5
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 5
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 5
- 230000006399 behavior Effects 0.000 description 5
- 230000007969 cellular immunity Effects 0.000 description 5
- 238000007405 data analysis Methods 0.000 description 5
- 238000002073 fluorescence micrograph Methods 0.000 description 5
- 230000007246 mechanism Effects 0.000 description 5
- 238000012544 monitoring process Methods 0.000 description 5
- 210000001616 monocyte Anatomy 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 5
- 108091033319 polynucleotide Proteins 0.000 description 5
- 102000040430 polynucleotide Human genes 0.000 description 5
- 239000002157 polynucleotide Substances 0.000 description 5
- 230000000770 proinflammatory effect Effects 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- OZFAFGSSMRRTDW-UHFFFAOYSA-N (2,4-dichlorophenyl) benzenesulfonate Chemical compound ClC1=CC(Cl)=CC=C1OS(=O)(=O)C1=CC=CC=C1 OZFAFGSSMRRTDW-UHFFFAOYSA-N 0.000 description 4
- 102000000844 Cell Surface Receptors Human genes 0.000 description 4
- 108010001857 Cell Surface Receptors Proteins 0.000 description 4
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 208000032612 Glial tumor Diseases 0.000 description 4
- 206010018338 Glioma Diseases 0.000 description 4
- 108060003951 Immunoglobulin Proteins 0.000 description 4
- 108090000174 Interleukin-10 Proteins 0.000 description 4
- 102000003814 Interleukin-10 Human genes 0.000 description 4
- 108091093037 Peptide nucleic acid Proteins 0.000 description 4
- 108010026552 Proteome Proteins 0.000 description 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 4
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 230000008568 cell cell communication Effects 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- 230000004069 differentiation Effects 0.000 description 4
- 239000000975 dye Substances 0.000 description 4
- 238000001506 fluorescence spectroscopy Methods 0.000 description 4
- 102000018358 immunoglobulin Human genes 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000011159 matrix material Substances 0.000 description 4
- 206010027191 meningioma Diseases 0.000 description 4
- 230000001617 migratory effect Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 102000005962 receptors Human genes 0.000 description 4
- 108020003175 receptors Proteins 0.000 description 4
- 230000011664 signaling Effects 0.000 description 4
- 238000002174 soft lithography Methods 0.000 description 4
- 238000007619 statistical method Methods 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 238000000954 titration curve Methods 0.000 description 4
- 239000005051 trimethylchlorosilane Substances 0.000 description 4
- 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 description 3
- 101000746373 Homo sapiens Granulocyte-macrophage colony-stimulating factor Proteins 0.000 description 3
- 102000017727 Immunoglobulin Variable Region Human genes 0.000 description 3
- 108010067060 Immunoglobulin Variable Region Proteins 0.000 description 3
- 108010074328 Interferon-gamma Proteins 0.000 description 3
- 108010002386 Interleukin-3 Proteins 0.000 description 3
- 108090000978 Interleukin-4 Proteins 0.000 description 3
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 3
- 102100030412 Matrix metalloproteinase-9 Human genes 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 229960002685 biotin Drugs 0.000 description 3
- 235000020958 biotin Nutrition 0.000 description 3
- 239000011616 biotin Substances 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 230000012292 cell migration Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000003086 colorant Substances 0.000 description 3
- 230000016396 cytokine production Effects 0.000 description 3
- 230000002939 deleterious effect Effects 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 3
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 201000005202 lung cancer Diseases 0.000 description 3
- 208000020816 lung neoplasm Diseases 0.000 description 3
- 230000003211 malignant effect Effects 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 230000001394 metastastic effect Effects 0.000 description 3
- 206010061289 metastatic neoplasm Diseases 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 238000007837 multiplex assay Methods 0.000 description 3
- 238000012634 optical imaging Methods 0.000 description 3
- 230000003076 paracrine Effects 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 108010054624 red fluorescent protein Proteins 0.000 description 3
- 238000012552 review Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 210000000130 stem cell Anatomy 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 239000012103 Alexa Fluor 488 Substances 0.000 description 2
- WHVNXSBKJGAXKU-UHFFFAOYSA-N Alexa Fluor 532 Chemical compound [H+].[H+].CC1(C)C(C)NC(C(=C2OC3=C(C=4C(C(C(C)N=4)(C)C)=CC3=3)S([O-])(=O)=O)S([O-])(=O)=O)=C1C=C2C=3C(C=C1)=CC=C1C(=O)ON1C(=O)CCC1=O WHVNXSBKJGAXKU-UHFFFAOYSA-N 0.000 description 2
- 108090001008 Avidin Proteins 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- PHEDXBVPIONUQT-UHFFFAOYSA-N Cocarcinogen A1 Natural products CCCCCCCCCCCCCC(=O)OC1C(C)C2(O)C3C=C(C)C(=O)C3(O)CC(CO)=CC2C2C1(OC(C)=O)C2(C)C PHEDXBVPIONUQT-UHFFFAOYSA-N 0.000 description 2
- 206010061818 Disease progression Diseases 0.000 description 2
- 238000012286 ELISA Assay Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 108050001049 Extracellular proteins Proteins 0.000 description 2
- 201000010915 Glioblastoma multiforme Diseases 0.000 description 2
- 102100039619 Granulocyte colony-stimulating factor Human genes 0.000 description 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 2
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 2
- 101000746367 Homo sapiens Granulocyte colony-stimulating factor Proteins 0.000 description 2
- 101001046686 Homo sapiens Integrin alpha-M Proteins 0.000 description 2
- 101000845170 Homo sapiens Thymic stromal lymphopoietin Proteins 0.000 description 2
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 2
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 2
- 102100022338 Integrin alpha-M Human genes 0.000 description 2
- 102100037850 Interferon gamma Human genes 0.000 description 2
- 108010065805 Interleukin-12 Proteins 0.000 description 2
- 102000013462 Interleukin-12 Human genes 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241000382301 Isoplexis Species 0.000 description 2
- 108010015302 Matrix metalloproteinase-9 Proteins 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 108010076504 Protein Sorting Signals Proteins 0.000 description 2
- 239000012980 RPMI-1640 medium Substances 0.000 description 2
- 102100031294 Thymic stromal lymphopoietin Human genes 0.000 description 2
- 102100039360 Toll-like receptor 4 Human genes 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 2
- 230000000712 assembly Effects 0.000 description 2
- 238000000429 assembly Methods 0.000 description 2
- 230000008267 autocrine signaling Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- KQNZDYYTLMIZCT-KQPMLPITSA-N brefeldin A Chemical compound O[C@@H]1\C=C\C(=O)O[C@@H](C)CCC\C=C\[C@@H]2C[C@H](O)C[C@H]21 KQNZDYYTLMIZCT-KQPMLPITSA-N 0.000 description 2
- JUMGSHROWPPKFX-UHFFFAOYSA-N brefeldin-A Natural products CC1CCCC=CC2(C)CC(O)CC2(C)C(O)C=CC(=O)O1 JUMGSHROWPPKFX-UHFFFAOYSA-N 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 230000023549 cell-cell signaling Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000002512 chemotherapy Methods 0.000 description 2
- 238000003759 clinical diagnosis Methods 0.000 description 2
- 238000007621 cluster analysis Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 230000021615 conjugation Effects 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- 108010082025 cyan fluorescent protein Proteins 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000010432 diamond Substances 0.000 description 2
- 230000005750 disease progression Effects 0.000 description 2
- 238000007876 drug discovery Methods 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000005294 ferromagnetic effect Effects 0.000 description 2
- 238000012921 fluorescence analysis Methods 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 239000005090 green fluorescent protein Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 235000003642 hunger Nutrition 0.000 description 2
- 239000012642 immune effector Substances 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 229940072221 immunoglobulins Drugs 0.000 description 2
- 229940121354 immunomodulator Drugs 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000007641 inkjet printing Methods 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 230000002601 intratumoral effect Effects 0.000 description 2
- 238000012417 linear regression Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 208000037819 metastatic cancer Diseases 0.000 description 2
- 208000011575 metastatic malignant neoplasm Diseases 0.000 description 2
- 230000004899 motility Effects 0.000 description 2
- 238000000879 optical micrograph Methods 0.000 description 2
- 230000014306 paracrine signaling Effects 0.000 description 2
- 230000005298 paramagnetic effect Effects 0.000 description 2
- 230000007030 peptide scission Effects 0.000 description 2
- 230000035790 physiological processes and functions Effects 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 230000004481 post-translational protein modification Effects 0.000 description 2
- 230000003389 potentiating effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 230000017854 proteolysis Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 238000003259 recombinant expression Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000018448 secretion by cell Effects 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000000527 sonication Methods 0.000 description 2
- 230000009870 specific binding Effects 0.000 description 2
- 238000004611 spectroscopical analysis Methods 0.000 description 2
- 230000037351 starvation Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 201000007003 transitional meningioma Diseases 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 230000004614 tumor growth Effects 0.000 description 2
- 238000010200 validation analysis Methods 0.000 description 2
- 238000012800 visualization Methods 0.000 description 2
- 235000012431 wafers Nutrition 0.000 description 2
- 108091005957 yellow fluorescent proteins Proteins 0.000 description 2
- LLTDOAPVRPZLCM-UHFFFAOYSA-O 4-(7,8,8,16,16,17-hexamethyl-4,20-disulfo-2-oxa-18-aza-6-azoniapentacyclo[11.7.0.03,11.05,9.015,19]icosa-1(20),3,5,9,11,13,15(19)-heptaen-12-yl)benzoic acid Chemical compound CC1(C)C(C)NC(C(=C2OC3=C(C=4C(C(C(C)[NH+]=4)(C)C)=CC3=3)S(O)(=O)=O)S(O)(=O)=O)=C1C=C2C=3C1=CC=C(C(O)=O)C=C1 LLTDOAPVRPZLCM-UHFFFAOYSA-O 0.000 description 1
- 102100026802 72 kDa type IV collagenase Human genes 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- 208000003174 Brain Neoplasms Diseases 0.000 description 1
- 108700012434 CCL3 Proteins 0.000 description 1
- 101150066577 CD14 gene Proteins 0.000 description 1
- 241000282465 Canis Species 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 102000000013 Chemokine CCL3 Human genes 0.000 description 1
- 102000001326 Chemokine CCL4 Human genes 0.000 description 1
- 108010055165 Chemokine CCL4 Proteins 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 101710112752 Cytotoxin Proteins 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- 229940033330 HIV vaccine Drugs 0.000 description 1
- 102100021866 Hepatocyte growth factor Human genes 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000627872 Homo sapiens 72 kDa type IV collagenase Proteins 0.000 description 1
- 101000959820 Homo sapiens Interferon alpha-1/13 Proteins 0.000 description 1
- 101000990902 Homo sapiens Matrix metalloproteinase-9 Proteins 0.000 description 1
- 101000946889 Homo sapiens Monocyte differentiation antigen CD14 Proteins 0.000 description 1
- 101000669447 Homo sapiens Toll-like receptor 4 Proteins 0.000 description 1
- 108091058560 IL8 Proteins 0.000 description 1
- 108010058683 Immobilized Proteins Proteins 0.000 description 1
- 238000012404 In vitro experiment Methods 0.000 description 1
- 102100040019 Interferon alpha-1/13 Human genes 0.000 description 1
- 102000008070 Interferon-gamma Human genes 0.000 description 1
- 108090000176 Interleukin-13 Proteins 0.000 description 1
- 108090000172 Interleukin-15 Proteins 0.000 description 1
- 108050003558 Interleukin-17 Proteins 0.000 description 1
- 102000013691 Interleukin-17 Human genes 0.000 description 1
- 108010002350 Interleukin-2 Proteins 0.000 description 1
- 102000013264 Interleukin-23 Human genes 0.000 description 1
- 108010065637 Interleukin-23 Proteins 0.000 description 1
- 108010066979 Interleukin-27 Proteins 0.000 description 1
- 108010002616 Interleukin-5 Proteins 0.000 description 1
- 108010002586 Interleukin-7 Proteins 0.000 description 1
- 108010002335 Interleukin-9 Proteins 0.000 description 1
- 102000000585 Interleukin-9 Human genes 0.000 description 1
- 102100020880 Kit ligand Human genes 0.000 description 1
- 102100028123 Macrophage colony-stimulating factor 1 Human genes 0.000 description 1
- 101710127797 Macrophage colony-stimulating factor 1 Proteins 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102100035877 Monocyte differentiation antigen CD14 Human genes 0.000 description 1
- 102100031789 Myeloid-derived growth factor Human genes 0.000 description 1
- 206010033307 Overweight Diseases 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 230000024932 T cell mediated immunity Effects 0.000 description 1
- 108700012920 TNF Proteins 0.000 description 1
- 108010060804 Toll-Like Receptor 4 Proteins 0.000 description 1
- 102000006747 Transforming Growth Factor alpha Human genes 0.000 description 1
- 108090001012 Transforming Growth Factor beta Proteins 0.000 description 1
- 102000004887 Transforming Growth Factor beta Human genes 0.000 description 1
- 101800004564 Transforming growth factor alpha Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 1
- 206010064390 Tumour invasion Diseases 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 241000269370 Xenopus <genus> Species 0.000 description 1
- 230000021736 acetylation Effects 0.000 description 1
- 238000006640 acetylation reaction Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 210000001130 astrocyte Anatomy 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000007321 biological mechanism Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 208000024055 brain glioblastoma Diseases 0.000 description 1
- 201000011609 brain glioblastoma multiforme Diseases 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 230000009400 cancer invasion Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 230000009087 cell motility Effects 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 230000033077 cellular process Effects 0.000 description 1
- 230000036755 cellular response Effects 0.000 description 1
- 239000002975 chemoattractant Substances 0.000 description 1
- 230000003399 chemotactic effect Effects 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000011281 clinical therapy Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000002508 contact lithography Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010219 correlation analysis Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 231100000599 cytotoxic agent Toxicity 0.000 description 1
- 239000002619 cytotoxin Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 229910003460 diamond Inorganic materials 0.000 description 1
- 238000003748 differential diagnosis Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003596 drug target Substances 0.000 description 1
- 238000002651 drug therapy Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 108091006047 fluorescent proteins Proteins 0.000 description 1
- 102000034287 fluorescent proteins Human genes 0.000 description 1
- 238000010230 functional analysis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000011331 genomic analysis Methods 0.000 description 1
- 230000002518 glial effect Effects 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 210000002443 helper t lymphocyte Anatomy 0.000 description 1
- 230000000423 heterosexual effect Effects 0.000 description 1
- 238000007417 hierarchical cluster analysis Methods 0.000 description 1
- 230000013632 homeostatic process Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000008611 intercellular interaction Effects 0.000 description 1
- 229960003130 interferon gamma Drugs 0.000 description 1
- 238000010212 intracellular staining Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000001459 lithography Methods 0.000 description 1
- 238000010859 live-cell imaging Methods 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000005291 magnetic effect Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 238000000813 microcontact printing Methods 0.000 description 1
- 210000000274 microglia Anatomy 0.000 description 1
- 230000002025 microglial effect Effects 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 230000003228 microsomal effect Effects 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000007498 myristoylation Effects 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 210000004498 neuroglial cell Anatomy 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 210000004287 null lymphocyte Anatomy 0.000 description 1
- 210000004248 oligodendroglia Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000035778 pathophysiological process Effects 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 238000011338 personalized therapy Methods 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 230000013823 prenylation Effects 0.000 description 1
- 238000002953 preparative HPLC Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000011092 protein amplification Methods 0.000 description 1
- 238000003498 protein array Methods 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 230000012846 protein folding Effects 0.000 description 1
- 230000005664 protein glycosylation in endoplasmic reticulum Effects 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 230000006337 proteolytic cleavage Effects 0.000 description 1
- 238000000575 proteomic method Methods 0.000 description 1
- 230000018612 quorum sensing Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 230000008844 regulatory mechanism Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000003118 sandwich ELISA Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000007811 spectroscopic assay Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 210000000603 stem cell niche Anatomy 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 230000028973 vesicle-mediated transport Effects 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/70—Mechanisms involved in disease identification
- G01N2800/7023—(Hyper)proliferation
- G01N2800/7028—Cancer
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261692895P | 2012-08-24 | 2012-08-24 | |
| US61/692,895 | 2012-08-24 | ||
| US201361779299P | 2013-03-13 | 2013-03-13 | |
| US61/779,299 | 2013-03-13 | ||
| CN201380055627.0A CN104884605B (zh) | 2012-08-24 | 2013-08-23 | 用于高通量多重检测的系统、装置和方法 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380055627.0A Division CN104884605B (zh) | 2012-08-24 | 2013-08-23 | 用于高通量多重检测的系统、装置和方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108267581A true CN108267581A (zh) | 2018-07-10 |
Family
ID=50150413
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810002554.3A Pending CN108267581A (zh) | 2012-08-24 | 2013-08-23 | 用于高通量多重检测的系统、装置和方法 |
| CN201380055627.0A Active CN104884605B (zh) | 2012-08-24 | 2013-08-23 | 用于高通量多重检测的系统、装置和方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380055627.0A Active CN104884605B (zh) | 2012-08-24 | 2013-08-23 | 用于高通量多重检测的系统、装置和方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (6) | US9188586B2 (https=) |
| EP (2) | EP4148118A1 (https=) |
| JP (3) | JP6197039B2 (https=) |
| CN (2) | CN108267581A (https=) |
| WO (1) | WO2014031997A1 (https=) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109991423A (zh) * | 2019-01-29 | 2019-07-09 | 厦门大学 | 高效单细胞捕获与快速单细胞分泌蛋白检测平台及检测方法 |
| CN112798785A (zh) * | 2019-11-13 | 2021-05-14 | 中国科学院大连化学物理研究所 | 一种可实现细胞捕获与多指标生物分析的芯片与应用 |
| CN113005022A (zh) * | 2020-07-06 | 2021-06-22 | 山东大学 | 一种用于同一个单细胞分泌蛋白、细胞质mRNA和核内DNA的同步检测芯片及方法 |
| CN113009145A (zh) * | 2020-06-17 | 2021-06-22 | 山东大学 | 一种用于治疗细胞分泌蛋白检测的微流控芯片及制备方法 |
| CN113008764A (zh) * | 2020-06-17 | 2021-06-22 | 山东大学 | 一种单细胞功能评估试剂盒及评估方法 |
| CN113030481A (zh) * | 2020-06-17 | 2021-06-25 | 山东大学 | 一种单细胞外泌体静态检测试剂盒及检测分析方法 |
| CN116930047A (zh) * | 2023-08-11 | 2023-10-24 | 中翰盛泰生物技术股份有限公司 | 一种用于流式荧光检测前处理装置及其处理方法 |
Families Citing this family (96)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009012340A2 (en) | 2007-07-16 | 2009-01-22 | California Institute Of Technology | Microfluidic devices, methods and systems for detecting target molecules |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US11435358B2 (en) | 2011-06-23 | 2022-09-06 | Board Of Regents, The University Of Texas System | Single molecule peptide sequencing |
| EP2739587B1 (en) | 2011-08-01 | 2020-05-27 | Denovo Sciences | Cell capture system |
| US10466160B2 (en) | 2011-08-01 | 2019-11-05 | Celsee Diagnostics, Inc. | System and method for retrieving and analyzing particles |
| US9404864B2 (en) | 2013-03-13 | 2016-08-02 | Denovo Sciences, Inc. | System for imaging captured cells |
| US10941396B2 (en) | 2012-02-27 | 2021-03-09 | Becton, Dickinson And Company | Compositions and kits for molecular counting |
| CN108267581A (zh) | 2012-08-24 | 2018-07-10 | 耶鲁大学 | 用于高通量多重检测的系统、装置和方法 |
| US9752181B2 (en) | 2013-01-26 | 2017-09-05 | Denovo Sciences, Inc. | System and method for capturing and analyzing cells |
| US9707562B2 (en) | 2013-03-13 | 2017-07-18 | Denovo Sciences, Inc. | System for capturing and analyzing cells |
| US10391490B2 (en) | 2013-05-31 | 2019-08-27 | Celsee Diagnostics, Inc. | System and method for isolating and analyzing cells |
| US9856535B2 (en) | 2013-05-31 | 2018-01-02 | Denovo Sciences, Inc. | System for isolating cells |
| DE102013211850A1 (de) * | 2013-06-21 | 2014-12-24 | Gilupi Gmbh | Schnelltest zum Nachweisen von Erregermaterial, insbesondere zur Unterstützung der Diagnose einer Sepsis, sowie Kit und Vorrichtung zur Durchführung eines Sepsistests |
| ES2711168T3 (es) | 2013-08-28 | 2019-04-30 | Becton Dickinson Co | Análisis masivo en paralelo de células individuales |
| FR3019899B1 (fr) * | 2014-04-09 | 2017-12-22 | Bio-Rad Innovations | Utilisation d'un colorant pour ameliorer la detection du signal dans un procede d'analyse |
| CN112782140A (zh) | 2014-12-03 | 2021-05-11 | 伊索普莱西斯公司 | 细胞分泌特征的分析和筛选 |
| CN107209934B (zh) * | 2014-12-03 | 2021-03-30 | 文塔纳医疗系统公司 | 用于定量分析异质生物标志物分布的方法、系统和装置 |
| EP3240885B1 (en) * | 2014-12-31 | 2024-11-13 | Fluidigm Canada Inc. | Structured biological samples for analysis by mass cytometry |
| WO2016115537A2 (en) | 2015-01-15 | 2016-07-21 | Massachusetts Institute Of Technology | Systems, methods, and apparatus for in vitro single-cell identification and recovery |
| US11353448B2 (en) | 2015-02-13 | 2022-06-07 | California Institute Of Technology | Methods and compositions for quantifying metabolites and proteins from single cells |
| WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| US10712356B2 (en) | 2015-04-21 | 2020-07-14 | General Automation Lab Technologies Inc. | Apparatus and method for picking biological sample |
| SG11201708579WA (en) * | 2015-04-21 | 2017-11-29 | Gen Automation Lab Tech Inc | High resolution systems, kits, apparatus, and methods for high throughput microbiology applications |
| TWI737614B (zh) | 2015-06-11 | 2021-09-01 | 博錸生技股份有限公司 | 已編碼微載體及其製造方法以及用以進行多工分析之包含該等已編碼微載體之套件 |
| CN108026524A (zh) | 2015-09-11 | 2018-05-11 | 赛卢拉研究公司 | 用于核酸文库标准化的方法和组合物 |
| JP6599472B2 (ja) | 2015-09-29 | 2019-10-30 | 東京応化工業株式会社 | 基板、構造体、構造体の製造方法、細胞の選別方法、細胞の製造方法、及び分泌物の産生方法 |
| US10436776B2 (en) | 2015-11-20 | 2019-10-08 | Plexbio Co., Ltd. | Methods and systems for selection of detection area |
| DK3384046T3 (da) * | 2015-12-01 | 2021-07-12 | Illumina Inc | Digitalt mikrofluidisk system til enkeltcelleisolering og karakterisering af analytter |
| US20200398277A9 (en) * | 2016-01-16 | 2020-12-24 | Spin Bio, Llc. | Tools and methods for isolation and analysis of individual components from a biological sample |
| US10019815B2 (en) | 2016-03-17 | 2018-07-10 | Plexbio Co., Ltd. | Methods and systems for image differentiated multiplex assays |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| EP3472351B1 (de) | 2016-06-15 | 2020-10-28 | Ludwig-Maximilians-Universität München | Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie |
| EP3510398B1 (en) | 2016-09-12 | 2026-02-25 | Isoplexis Corporation | System and methods for multiplexed analysis of cellular and other immunotherapeutics |
| US11796535B2 (en) | 2016-09-16 | 2023-10-24 | Plexbio Co., Ltd. | Methods and systems for multiplex assays |
| CA3034924C (en) | 2016-09-26 | 2025-11-18 | Becton, Dickinson And Company | MEASUREMENT OF PROTEIN EXPRESSION USING REAGENTS WITH BARCODED OLIGONUCLEOTIDE SEQUENCES |
| WO2018061733A1 (ja) * | 2016-09-28 | 2018-04-05 | 富士フイルム株式会社 | 検査方法および検査チップ |
| JP2020524480A (ja) | 2016-11-03 | 2020-08-20 | セル マイクロシステムズ インクＣｅｌｌ Ｍｉｃｒｏｓｙｓｔｅｍｓ， Ｉｎｃ． | 特定の数の細胞の自動収集 |
| EP4036578A1 (en) * | 2016-11-11 | 2022-08-03 | Isoplexis Corporation | Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells |
| CN110226084A (zh) | 2016-11-22 | 2019-09-10 | 伊索普莱克西斯公司 | 用于细胞捕获的系统、装置和方法，以及其制造方法 |
| CN110382708A (zh) | 2017-02-01 | 2019-10-25 | 赛卢拉研究公司 | 使用阻断性寡核苷酸进行选择性扩增 |
| EP3580354A4 (en) | 2017-02-13 | 2021-03-31 | Yale University | HIGH THROUGHPUT SINGLE CELL POLYOMICS |
| WO2018179735A1 (ja) * | 2017-03-28 | 2018-10-04 | ソニー株式会社 | 細胞の生産能力を分析するためのデバイス、細胞の生産能力を分析するための装置、細胞の生産能力を分析する方法及び細胞の生産能力を分析するためのシステム |
| JP7536450B2 (ja) | 2017-06-05 | 2024-08-20 | ベクトン・ディキンソン・アンド・カンパニー | 単一細胞用のサンプルインデックス付加 |
| WO2019046307A1 (en) * | 2017-08-29 | 2019-03-07 | Celsee Diagnostics, Inc. | SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS |
| WO2019088904A1 (en) * | 2017-10-31 | 2019-05-09 | Astrego Diagnostics Ab | Microfluidic device for cell characterization |
| US12122988B2 (en) | 2018-03-12 | 2024-10-22 | Sony Corporation | Cell evaluation device and cell evaluation system |
| CN110343655A (zh) * | 2018-04-02 | 2019-10-18 | 中国科学院大连化学物理研究所 | 一种集成细胞捕获、三维基底培养及生物分析的芯片 |
| CA3097976A1 (en) | 2018-05-03 | 2019-11-07 | Becton, Dickinson And Company | High throughput multiomics sample analysis |
| EP3788170B1 (en) | 2018-05-03 | 2025-01-01 | Becton, Dickinson and Company | Molecular barcoding on opposite transcript ends |
| CN110499251A (zh) * | 2018-05-17 | 2019-11-26 | 中国科学院大连化学物理研究所 | 高通量多参数的单细胞源性细胞外囊泡分析芯片及应用 |
| AU2019301750A1 (en) | 2018-07-12 | 2021-01-28 | Board Of Regents, The University Of Texas System | Molecular neighborhood detection by oligonucleotides |
| JP7172344B2 (ja) * | 2018-09-20 | 2022-11-16 | 東洋紡株式会社 | 分析方法および分析用装置 |
| ES2992135T3 (es) | 2018-10-01 | 2024-12-09 | Becton Dickinson Co | Determinar secuencias de transcripción 5 |
| GB2593091B (en) | 2018-10-05 | 2023-12-20 | Univ Texas | Solid-phase N-terminal peptide capture and release |
| CN109521202A (zh) * | 2018-10-31 | 2019-03-26 | 江苏师范大学 | 一种基于数字免疫分析技术的低丰度蛋白质绝对定量方法 |
| JP7618548B2 (ja) | 2018-11-08 | 2025-01-21 | ベクトン・ディキンソン・アンド・カンパニー | ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析 |
| WO2020117975A1 (en) * | 2018-12-04 | 2020-06-11 | IsoPlexis Corporation | Systems, devices and methods for identification, selective ablation, and selection and collection of single cells |
| EP3894590B1 (en) * | 2018-12-10 | 2025-10-22 | 10X Genomics, Inc. | Methods of using master / copy arrays for spatial detection |
| US11492660B2 (en) | 2018-12-13 | 2022-11-08 | Becton, Dickinson And Company | Selective extension in single cell whole transcriptome analysis |
| CN109765356A (zh) * | 2019-01-18 | 2019-05-17 | 江苏医联生物科技有限公司 | 一种蛋白质芯片荧光检测方法 |
| EP4242322B1 (en) | 2019-01-23 | 2024-08-21 | Becton, Dickinson and Company | Oligonucleotides associated with antibodies |
| CN113454234B (zh) | 2019-02-14 | 2025-03-18 | 贝克顿迪金森公司 | 杂合体靶向和全转录物组扩增 |
| US10633693B1 (en) | 2019-04-16 | 2020-04-28 | Celsee Diagnostics, Inc. | System and method for leakage control in a particle capture system |
| EP3966307B1 (en) | 2019-05-07 | 2024-11-20 | Bio-Rad Laboratories, Inc. | System and method for automated single cell processing |
| US11273439B2 (en) | 2019-05-07 | 2022-03-15 | Bio-Rad Laboratories, Inc. | System and method for target material retrieval from microwells |
| SG11202112898WA (en) | 2019-06-14 | 2021-12-30 | Bio Rad Laboratories | System and method for automated single cell processing and analyses |
| CN120099137A (zh) | 2019-07-22 | 2025-06-06 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| CN114787348B (zh) | 2019-09-30 | 2025-09-19 | 耶鲁大学 | 用于空间组学测序的确定性条形码 |
| JP7522189B2 (ja) | 2019-11-08 | 2024-07-24 | ベクトン・ディキンソン・アンド・カンパニー | 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用 |
| EP4090764A1 (en) | 2020-01-13 | 2022-11-23 | Becton, Dickinson and Company | Cell capture using du-containing oligonucleotides |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| CN115298322A (zh) * | 2020-01-17 | 2022-11-04 | 贝克顿迪金森公司 | 用于单细胞分泌组学的方法和组合物 |
| WO2021154230A1 (en) | 2020-01-29 | 2021-08-05 | Hewlett-Packard Development Company, L.P. | Antibody fluid deposits |
| WO2021155057A1 (en) | 2020-01-29 | 2021-08-05 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| US12064768B2 (en) | 2020-02-21 | 2024-08-20 | Northeastern University | Single cell isolation and processing system with reversible well shape |
| EP4111168A1 (en) | 2020-02-25 | 2023-01-04 | Becton Dickinson and Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| US11504719B2 (en) | 2020-03-12 | 2022-11-22 | Bio-Rad Laboratories, Inc. | System and method for receiving and delivering a fluid for sample processing |
| WO2021230799A1 (en) * | 2020-05-14 | 2021-11-18 | Camsund Daniel | A microfluidic device having specifically designed detection chambers |
| EP4150118A1 (en) | 2020-05-14 | 2023-03-22 | Becton Dickinson and Company | Primers for immune repertoire profiling |
| US12157913B2 (en) | 2020-06-02 | 2024-12-03 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| CN113009144B (zh) * | 2020-06-17 | 2023-07-21 | 山东大学 | 一种基于微流控技术的抗体检测试剂盒及检测方法 |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| CA3186058A1 (en) * | 2020-07-27 | 2022-02-03 | Benjamin Marc Feder Fogelson | Techniques for analyzing and detecting executional artifacts in microwell plates |
| WO2022026909A1 (en) | 2020-07-31 | 2022-02-03 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| WO2022125701A1 (en) * | 2020-12-09 | 2022-06-16 | Becton, Dickinson And Company | Multiplexed single cell immunoassay |
| EP4263859A1 (en) * | 2020-12-15 | 2023-10-25 | Becton, Dickinson and Company | Single cell secretome analysis |
| US12576400B2 (en) | 2021-01-08 | 2026-03-17 | Cellanome, Inc. | Methods for incubating and analyzing a cell in a compartment of a fluidic device |
| JP2024502618A (ja) | 2021-01-08 | 2024-01-22 | セラノーム， インコーポレイテッド | 生体試料を分析するためのデバイスおよび方法 |
| WO2022178253A1 (en) | 2021-02-19 | 2022-08-25 | IsoPlexis Corporation | Methods and devices for spatially resolved analysis of proteomic and genetic information |
| WO2022182832A1 (en) * | 2021-02-24 | 2022-09-01 | Board Of Regents, The University Of Texas System | Methods for polypeptide processing and analysis |
| JP2024527371A (ja) * | 2021-07-08 | 2024-07-24 | オウロテック インコーポレイテッド | 処置の有効性予測システム及び方法 |
| CN114073996B (zh) * | 2021-11-24 | 2023-04-14 | 中山大学 | 一种嵌套式微井阵列芯片及其制备方法 |
| WO2023107539A1 (en) | 2021-12-10 | 2023-06-15 | Bio-Rad Laboratories, Inc. | Compositions, methods, and systems for sample processing with morphology-adjustable functionalized particles |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060013031A1 (en) * | 2001-10-26 | 2006-01-19 | Vitra Bioscience, Inc. | Assay systems with adjustable fluid communication |
Family Cites Families (97)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6943034B1 (en) | 1991-11-22 | 2005-09-13 | Affymetrix, Inc. | Combinatorial strategies for polymer synthesis |
| EP0821726B1 (en) * | 1995-03-10 | 2014-05-07 | Meso Scale Technologies, LLC. | Multi-array, multi-specific electrochemiluminescence testing |
| US6632436B2 (en) | 1996-01-25 | 2003-10-14 | Genitrix Llc | Vaccine compositions and method of modulating immune responses |
| US6165739A (en) | 1996-03-13 | 2000-12-26 | Compucyte Corporation | Multiple simultaneous testing media |
| US6039897A (en) | 1996-08-28 | 2000-03-21 | University Of Washington | Multiple patterned structures on a single substrate fabricated by elastomeric micro-molding techniques |
| US6924153B1 (en) | 1997-03-06 | 2005-08-02 | Quidel Corporation | Quantitative lateral flow assays and devices |
| US5858801A (en) | 1997-03-13 | 1999-01-12 | The United States Of America As Represented By The Secretary Of The Navy | Patterning antibodies on a surface |
| US6103489A (en) | 1997-03-21 | 2000-08-15 | University Of Hawaii | Cell-free protein synthesis system with protein translocation and processing |
| US6613512B1 (en) | 1997-06-09 | 2003-09-02 | Caliper Technologies Corp. | Apparatus and method for correcting for variable velocity in microfluidic systems |
| US20030096232A1 (en) | 1997-12-19 | 2003-05-22 | Kris Richard M. | High throughput assay system |
| US6210910B1 (en) * | 1998-03-02 | 2001-04-03 | Trustees Of Tufts College | Optical fiber biosensor array comprising cell populations confined to microcavities |
| US6316193B1 (en) | 1998-10-06 | 2001-11-13 | Origene Technologies, Inc. | Rapid-screen cDNA library panels |
| US6429027B1 (en) * | 1998-12-28 | 2002-08-06 | Illumina, Inc. | Composite arrays utilizing microspheres |
| DE60045917D1 (de) * | 1999-02-23 | 2011-06-16 | Caliper Life Sciences Inc | Sequenzierung durch inkorporation |
| US8008071B2 (en) | 1999-11-08 | 2011-08-30 | University Of South Florida | Compositions and methods for detecting intracellular glucose and analogs thereof |
| US6875619B2 (en) | 1999-11-12 | 2005-04-05 | Motorola, Inc. | Microfluidic devices comprising biochannels |
| US20020090649A1 (en) | 1999-12-15 | 2002-07-11 | Tony Chan | High density column and row addressable electrode arrays |
| US6699665B1 (en) * | 2000-11-08 | 2004-03-02 | Surface Logix, Inc. | Multiple array system for integrating bioarrays |
| US7351575B2 (en) * | 2000-11-08 | 2008-04-01 | Surface Logix, Inc. | Methods for processing biological materials using peelable and resealable devices |
| WO2002044695A1 (en) | 2000-11-16 | 2002-06-06 | Burstein Technologies, Inc. | Methods and apparatus for detecting and quantifying lymphocytes with optical biodiscs |
| US20020100714A1 (en) | 2001-01-31 | 2002-08-01 | Sau Lan Tang Staats | Microfluidic devices |
| US7968305B2 (en) | 2001-03-24 | 2011-06-28 | Aviva Biosciences Corporation | Biochips including ion transport detecting structures and methods of use |
| US20020146745A1 (en) | 2001-04-03 | 2002-10-10 | Surromed, Inc. | Methods and reagents for multiplexed analyte capture, surface array self-assembly, and analysis of complex biological samples |
| DE10127221A1 (de) | 2001-05-23 | 2002-11-28 | Lifebits Ag | Träger für chemische, biochemische und biologische Substanzen |
| US7473767B2 (en) | 2001-07-03 | 2009-01-06 | The Institute For Systems Biology | Methods for detection and quantification of analytes in complex mixtures |
| US7023007B2 (en) | 2001-07-17 | 2006-04-04 | Caliper Life Sciences, Inc. | Methods and systems for alignment of detection optics |
| US10539561B1 (en) | 2001-08-30 | 2020-01-21 | Customarray, Inc. | Enzyme-amplified redox microarray detection process |
| US20050032144A1 (en) | 2001-11-16 | 2005-02-10 | Lombardi Vincent C | Method for selective conjugation of analytes to enzymes without unwanted enzyme-enzyme cross-linking |
| US7258837B2 (en) | 2001-12-05 | 2007-08-21 | University Of Washington | Microfluidic device and surface decoration process for solid phase affinity binding assays |
| US20030190689A1 (en) | 2002-04-05 | 2003-10-09 | Cell Signaling Technology,Inc. | Molecular profiling of disease and therapeutic response using phospho-specific antibodies |
| AU2002952384A0 (en) | 2002-10-31 | 2002-11-14 | Swinburne University Of Technology | Structures |
| CN2577283Y (zh) * | 2002-11-08 | 2003-10-01 | 穆海东 | 一种蛋白质芯片 |
| DE10305050A1 (de) | 2003-02-07 | 2004-08-19 | Roche Diagnostics Gmbh | Analytisches Testelement und Verfahren für Blutuntersuchungen |
| US7312197B2 (en) | 2003-02-24 | 2007-12-25 | University Of Maryland, Baltimore | Method of modifying glucose activity using polypeptides selectively expressed in fat tissue |
| US7452678B2 (en) | 2003-06-24 | 2008-11-18 | Bristol-Myers Squibb Company | Identification of biomarkers for liver toxicity |
| DE602004024539D1 (de) | 2003-07-03 | 2010-01-21 | Gentron Llc | Verfahren und systeme zur diagnose von nicht das zentrale nervensystem (zns) betreffenden krankheiten in zns-proben |
| US20050226779A1 (en) | 2003-09-19 | 2005-10-13 | Oldham Mark F | Vacuum assist for a microplate |
| US7981362B2 (en) | 2003-11-04 | 2011-07-19 | Meso Scale Technologies, Llc | Modular assay plates, reader systems and methods for test measurements |
| JP4814103B2 (ja) | 2003-11-12 | 2011-11-16 | バイオ−ラッド・ハイファ・リミテッド | アレイフォーマットにおいて複数の結合反応を実行するためのシステムおよび方法 |
| JP4880484B2 (ja) | 2004-02-20 | 2012-02-22 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 唾液ｍＲＮＡプロファイリング、バイオマーカーならびに関連した方法およびキット |
| JP4850061B2 (ja) | 2004-03-18 | 2012-01-11 | 日水製薬株式会社 | 抗原の分析装置の製造方法及び分析装置 |
| GB0409771D0 (en) * | 2004-04-30 | 2004-06-09 | Mabtech Ab | Assay |
| DE102004056735A1 (de) * | 2004-11-09 | 2006-07-20 | Clondiag Chip Technologies Gmbh | Vorrichtung für die Durchführung und Analyse von Mikroarray-Experimenten |
| CN1296492C (zh) | 2004-11-18 | 2007-01-24 | 博奥生物有限公司 | 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法 |
| EP1838880A2 (en) | 2005-01-21 | 2007-10-03 | Third Wave Technologies, Inc. | Methods and compositions for increased dynamic range detection of nucleic acid molecules |
| GB0508983D0 (en) | 2005-05-03 | 2005-06-08 | Oxford Gene Tech Ip Ltd | Cell analyser |
| CA2607579A1 (en) | 2005-05-06 | 2006-11-23 | The Regents Of The University Of California | Microfluidic system for identifying or sizing individual particles passing through a channel |
| US20060263818A1 (en) | 2005-05-23 | 2006-11-23 | Axel Scherer | High throughput multi-antigen microfluidic fluorescence immunoassays |
| US20080220982A1 (en) | 2005-07-26 | 2008-09-11 | Vu Tania Q | Nanoparticle Probes for Capture, Sorting and Placement of Targets |
| US20100297145A1 (en) | 2005-08-04 | 2010-11-25 | Kazutake Tsujikawa | Apoptosis promoter, cell proliferation inhibitor, prophylactic/therapeutic agent for cancer, screening method for the promoter, inhibitor or agent |
| WO2007033385A2 (en) | 2005-09-13 | 2007-03-22 | Fluidigm Corporation | Microfluidic assay devices and methods |
| WO2007035633A2 (en) * | 2005-09-16 | 2007-03-29 | President & Fellows Of Harvard College | Screening assays and methods |
| US20070122819A1 (en) | 2005-11-25 | 2007-05-31 | Industrial Technology Research Institute | Analyte assay structure in microfluidic chip for quantitative analysis and method for using the same |
| WO2007076023A2 (en) * | 2005-12-21 | 2007-07-05 | Meso Scale Technologies, Llc | Assay modules having assay reagents and methods of making and using same |
| US20100009335A1 (en) | 2006-02-07 | 2010-01-14 | Victor Joseph | Temperature-regulated culture plates |
| US8492098B2 (en) | 2006-02-21 | 2013-07-23 | The Trustees Of Tufts College | Methods and arrays for target analyte detection and determination of reaction components that affect a reaction |
| AU2007281479A1 (en) | 2006-08-02 | 2008-02-07 | California Institute Of Technology | Methods and systems for detecting and/or sorting targets |
| US8394590B2 (en) | 2006-08-02 | 2013-03-12 | California Institute Of Technology | Capture agents and related methods and systems for detecting and/or sorting targets |
| WO2008101196A1 (en) | 2007-02-15 | 2008-08-21 | Osmetech Molecular Diagnostics | Fluidics devices |
| JP2009002806A (ja) | 2007-06-21 | 2009-01-08 | Hitachi Ltd | 化学発光計測装置 |
| WO2009012340A2 (en) | 2007-07-16 | 2009-01-22 | California Institute Of Technology | Microfluidic devices, methods and systems for detecting target molecules |
| WO2009105583A1 (en) | 2008-02-19 | 2009-08-27 | Anstron Technologies Company | Fluorescence resonance energy transfer (fret) binding assays that are surface-based |
| US8871527B2 (en) | 2008-04-29 | 2014-10-28 | Becton, Dickinson And Company | Method and system for measuring a sample to determine the presence of and optionally treat a pathologic condition |
| JP5777045B2 (ja) | 2008-09-02 | 2015-09-09 | 国立研究開発法人産業技術総合研究所 | 細胞検出方法及び該方法に用いるマイクロアレイチップ |
| JP2010066146A (ja) | 2008-09-11 | 2010-03-25 | Panasonic Corp | マイクロアレイ測定方法 |
| KR101335725B1 (ko) * | 2008-10-02 | 2013-12-04 | 삼성전자주식회사 | 다중분석용 미세유동 구조물 및 이를 포함하는 미세유동 장치 |
| EP2370814B1 (en) * | 2008-12-04 | 2015-04-29 | Massachusetts Institute of Technology | Method for diagnosing allergic reactions |
| US9244071B2 (en) * | 2009-01-21 | 2016-01-26 | Massachusetts Institute Of Technology | Compositions and methods for assessing cytotoxicity of single cells |
| US8317737B2 (en) | 2009-02-25 | 2012-11-27 | The Invention Science Fund I, Llc | Device for actively removing a target component from blood or lymph of a vertebrate subject |
| EP2473857B1 (en) | 2009-09-01 | 2021-09-29 | Corsolutions, LLC | Microfluidic interface |
| US9500645B2 (en) | 2009-11-23 | 2016-11-22 | Cyvek, Inc. | Micro-tube particles for microfluidic assays and methods of manufacture |
| WO2012022482A1 (en) * | 2010-08-20 | 2012-02-23 | Eth Zurich | Microwell arrays for direct quantification of analytes on a flat sample |
| CN103917870B (zh) * | 2011-11-16 | 2016-04-13 | 贝克顿·迪金森公司 | 用于检测样品中的分析物的方法和系统 |
| CA2856868C (en) | 2011-12-13 | 2020-07-07 | Single Cell Technology, Inc. | Method of screening a plurality of single secreting cells for functional activity |
| IN2014DN07531A (https=) | 2012-03-30 | 2015-04-24 | Clarient Diagnostic Services Inc | |
| CN102690786B (zh) | 2012-06-05 | 2014-02-12 | 武汉格蓝丽富科技有限公司 | 一种细胞富集、分离、提取的方法和仪器及单细胞分析方法 |
| CN108267581A (zh) | 2012-08-24 | 2018-07-10 | 耶鲁大学 | 用于高通量多重检测的系统、装置和方法 |
| CA3088591C (en) | 2012-09-28 | 2023-01-03 | Vibrant Holdings, Llc | Inverted pillar plates for assaying microarrays and methods of using same. |
| US20140307931A1 (en) | 2013-04-15 | 2014-10-16 | Massachusetts Institute Of Technology | Fully automated system and method for image segmentation and quality control of protein microarrays |
| US20160122753A1 (en) | 2013-06-12 | 2016-05-05 | Tarjei Mikkelsen | High-throughput rna-seq |
| EP3010550B1 (en) | 2013-06-20 | 2021-10-27 | California Institute of Technology | Cyclic peptides as protein targeting agents |
| US10112195B2 (en) | 2013-07-31 | 2018-10-30 | Hitachi High-Technologies Corporation | Flow cell for nucleic acid analysis and nucleic acid analyzer |
| US9498778B2 (en) | 2014-11-11 | 2016-11-22 | Genmark Diagnostics, Inc. | Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system |
| ES2895750T3 (es) | 2014-09-15 | 2022-02-22 | Abvitro Llc | Secuenciación de alto rendimiento de colecciones de nucleótidos |
| US20160108458A1 (en) | 2014-10-06 | 2016-04-21 | The Board Of Trustees Of The Leland Stanford Junior University | Multiplexed detection and quantification of nucleic acids in single-cells |
| KR20170068504A (ko) | 2014-10-08 | 2017-06-19 | 노파르티스 아게 | 키메라 항원 수용체 요법에 대한 치료 반응성을 예측하는 바이오마커 및 그의 용도 |
| CN112782140A (zh) | 2014-12-03 | 2021-05-11 | 伊索普莱西斯公司 | 细胞分泌特征的分析和筛选 |
| AU2015357526B2 (en) | 2014-12-05 | 2022-03-17 | Eureka Therapeutics, Inc. | Chimeric antigen receptors targeting B-cell maturation antigen and uses thereof |
| US11353448B2 (en) | 2015-02-13 | 2022-06-07 | California Institute Of Technology | Methods and compositions for quantifying metabolites and proteins from single cells |
| WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| CN108449933A (zh) | 2015-11-18 | 2018-08-24 | 宝生物工程（美国)有限公司 | 用于汇集来自多孔装置的样品的系统和方法 |
| CN110100010B (zh) | 2016-07-18 | 2024-03-08 | 耶鲁大学 | 单细胞中细胞内或表面分子靶标的多重检测 |
| EP3510398B1 (en) | 2016-09-12 | 2026-02-25 | Isoplexis Corporation | System and methods for multiplexed analysis of cellular and other immunotherapeutics |
| EP4036578A1 (en) | 2016-11-11 | 2022-08-03 | Isoplexis Corporation | Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells |
| CN110226084A (zh) | 2016-11-22 | 2019-09-10 | 伊索普莱克西斯公司 | 用于细胞捕获的系统、装置和方法，以及其制造方法 |
| EP3580354A4 (en) | 2017-02-13 | 2021-03-31 | Yale University | HIGH THROUGHPUT SINGLE CELL POLYOMICS |
| CA3056414A1 (en) | 2017-03-16 | 2018-09-20 | Case Western Reserve University | Biochip having microchannel provided with capturing agent for performing cytological analysis |
-
2013
- 2013-08-23 CN CN201810002554.3A patent/CN108267581A/zh active Pending
- 2013-08-23 EP EP22155232.6A patent/EP4148118A1/en active Pending
- 2013-08-23 CN CN201380055627.0A patent/CN104884605B/zh active Active
- 2013-08-23 JP JP2015528698A patent/JP6197039B2/ja active Active
- 2013-08-23 WO PCT/US2013/056454 patent/WO2014031997A1/en not_active Ceased
- 2013-08-23 EP EP13830534.7A patent/EP2888349B1/en active Active
-
2014
- 2014-12-05 US US14/562,061 patent/US9188586B2/en active Active
-
2015
- 2015-02-23 US US14/629,164 patent/US9506917B2/en active Active
-
2016
- 2016-10-11 US US15/290,874 patent/US10274486B2/en active Active
-
2017
- 2017-08-21 JP JP2017158313A patent/JP6620130B2/ja not_active Expired - Fee Related
-
2019
- 2019-03-06 US US16/294,853 patent/US10983116B2/en active Active
- 2019-09-10 JP JP2019164396A patent/JP2020046423A/ja active Pending
-
2021
- 2021-03-17 US US17/204,279 patent/US12105086B2/en active Active
-
2024
- 2024-08-19 US US18/808,943 patent/US20250116662A1/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060013031A1 (en) * | 2001-10-26 | 2006-01-19 | Vitra Bioscience, Inc. | Assay systems with adjustable fluid communication |
Non-Patent Citations (2)
| Title |
|---|
| CHAO MA等: "A clinical microchip for evaluation of single immune cells reveals high functional heterogeneity in phenotypically similar T cells", 《NATURE MEDICINE》 * |
| HAN QING ET AL: "Multidimensional analysis of the frequencies and rates of cytokine secretion from single cells by quantitative microengraving", 《LAB ON A CHIP,ROYAL SOCIETY OF CHEMISTRY》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109991423A (zh) * | 2019-01-29 | 2019-07-09 | 厦门大学 | 高效单细胞捕获与快速单细胞分泌蛋白检测平台及检测方法 |
| CN109991423B (zh) * | 2019-01-29 | 2020-06-26 | 厦门大学 | 高效单细胞捕获与快速单细胞分泌蛋白检测平台及检测方法 |
| CN112798785A (zh) * | 2019-11-13 | 2021-05-14 | 中国科学院大连化学物理研究所 | 一种可实现细胞捕获与多指标生物分析的芯片与应用 |
| CN113009145A (zh) * | 2020-06-17 | 2021-06-22 | 山东大学 | 一种用于治疗细胞分泌蛋白检测的微流控芯片及制备方法 |
| CN113008764A (zh) * | 2020-06-17 | 2021-06-22 | 山东大学 | 一种单细胞功能评估试剂盒及评估方法 |
| CN113030481A (zh) * | 2020-06-17 | 2021-06-25 | 山东大学 | 一种单细胞外泌体静态检测试剂盒及检测分析方法 |
| CN113005022A (zh) * | 2020-07-06 | 2021-06-22 | 山东大学 | 一种用于同一个单细胞分泌蛋白、细胞质mRNA和核内DNA的同步检测芯片及方法 |
| CN113005022B (zh) * | 2020-07-06 | 2024-04-26 | 山东大学 | 一种用于同一个单细胞分泌蛋白、细胞质mRNA和核内DNA的同步检测芯片及方法 |
| CN116930047A (zh) * | 2023-08-11 | 2023-10-24 | 中翰盛泰生物技术股份有限公司 | 一种用于流式荧光检测前处理装置及其处理方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP4148118A1 (en) | 2023-03-15 |
| JP2020046423A (ja) | 2020-03-26 |
| EP2888349A1 (en) | 2015-07-01 |
| US12105086B2 (en) | 2024-10-01 |
| US9506917B2 (en) | 2016-11-29 |
| US20150204864A1 (en) | 2015-07-23 |
| CN104884605B (zh) | 2018-05-18 |
| US20220057388A1 (en) | 2022-02-24 |
| CN104884605A (zh) | 2015-09-02 |
| EP2888349B1 (en) | 2022-02-09 |
| JP6620130B2 (ja) | 2019-12-11 |
| US9188586B2 (en) | 2015-11-17 |
| US10983116B2 (en) | 2021-04-20 |
| WO2014031997A1 (en) | 2014-02-27 |
| US20190324028A1 (en) | 2019-10-24 |
| JP6197039B2 (ja) | 2017-09-13 |
| US20250116662A1 (en) | 2025-04-10 |
| US10274486B2 (en) | 2019-04-30 |
| EP2888349A4 (en) | 2016-03-02 |
| JP2015527588A (ja) | 2015-09-17 |
| US20170067887A1 (en) | 2017-03-09 |
| US20150204862A1 (en) | 2015-07-23 |
| JP2018021917A (ja) | 2018-02-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104884605B (zh) | 用于高通量多重检测的系统、装置和方法 | |
| CN102308213B (zh) | 诊断过敏性反应的方法 | |
| Lu et al. | High-throughput secretomic analysis of single cells to assess functional cellular heterogeneity | |
| KR101679869B1 (ko) | 생물학적 활성제의 생산을 위한 단일 세포의 스크리닝 방법 | |
| Rodriguez-Moncayo et al. | Integrated microfluidic device for functional secretory immunophenotyping of immune cells | |
| JP2004173681A (ja) | 抗原特異的リンパ球検出用マイクロウェルアレイチップ、抗原特異的リンパ球の検出法及び製造方法 | |
| Abdullah et al. | Ultrasimple single-cell detection of multiple cytokines by a nanowell chip integrated with encoded microarrays | |
| US20130149724A1 (en) | Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells | |
| US20190024033A1 (en) | Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells | |
| Choi | Advances in single cell technologies in immunology | |
| Zhu et al. | High-throughput single-cell extracellular vesicle secretion analysis on a desktop scanner without cell counting | |
| Gao et al. | Quantitatively evaluating interactions between patient-derived organoids and autologous immune cells by microfluidic chip | |
| RU2682721C2 (ru) | Биологический микрочип для обнаружения опухолевых экзосом в сыворотке крови человека для диагностики колоректального рака | |
| US20120058507A1 (en) | Clonal Derivation and Cell Culture quality Control Screening Methods | |
| Li et al. | Microfluidic chip for cancer cell detection and diagnosis | |
| Lussier et al. | PiP‐Plex: A Particle‐in‐Particle System for Multiplexed Quantification of Proteins Secreted by Single Cells | |
| Young | Relating Mechanical and Genetic Data at Single Cell Level across the Genome to Investigate Metastasis | |
| Abdullah | Novel Functional Proteomics Techniques for Multiplex and Single Cell Cytokines Detection | |
| Shilts | Technologies to decode the multicellular networks within the human body | |
| CN120254242A (zh) | 外泌体多种生物标志物的高通量检测芯片及其制备方法和应用 | |
| Vermesh | Technologies for Protein Analysis and Tissue Engineering, with Applications in Cancer | |
| Chingozha | New Microfluidic Systems for Single-Cell Signaling Studies in Immune Cells | |
| Roupioz | Antibody Microarrays for Cell-Based Assays: The Use of Micro-Arrayed Antibodies for Exploring Cell Surface Diversity or Whole Cell Functionality | |
| DO-IT-YOURSELF | Proteomics in multiplex |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180710 |