CN108124618A - A kind of method that half Water culture of black fruit fructus lycii is taken root - Google Patents
A kind of method that half Water culture of black fruit fructus lycii is taken root Download PDFInfo
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- CN108124618A CN108124618A CN201711477391.6A CN201711477391A CN108124618A CN 108124618 A CN108124618 A CN 108124618A CN 201711477391 A CN201711477391 A CN 201711477391A CN 108124618 A CN108124618 A CN 108124618A
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- branch
- fructus lycii
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The present invention provides a kind of methods that half Water culture of black fruit fructus lycii is taken root, and belong to black fruit fructus lycii cuttage technique field, the described method comprises the following steps：1) concussion processing black fruit fructus lycii 7~9h of branch at 26~30 DEG C will be obtained after concussion treated branch is placed in 6~9 DEG C of 0.5~1.5h of low-temperature treatment and be treated cuttage branch；2) treat that culture of rootage 40d~50d is carried out in cuttage branch cutting to matrix obtains field-transplanting seedling by described；Solution of taking root is poured to matrix in the process of rooting culture, it is 1 to keep the mass volume ratio (g/ml) of matrix and solution of taking root：(2~3).Rooting rate can reach 100% after the method black fruit fructus lycii branch cutting, and root growth is healthy and strong, and 40d~50d can obtain field-transplanting seedling；Simple to operate, growing-seedling period is short, and seedling speed is fast, can be applied to the industrialization breeding of black fruit fructus lycii nursery stock.
Description
Technical field
The invention belongs to black fruit fructus lycii cuttage technique fields, and in particular to the side that a kind of half Water culture of black fruit fructus lycii is taken root
Method.
Background technology
Black fruit fructus lycii (Lyciumruthencium), belongs to Solanaceae Lycium, and for more quil shrubs, multi-branched, branch is hard
Firmly, often in bending in a zigzag, white.China's western region is distributed mainly on, is the main of Chinese Desert Area zonal vegetation
Group seeds are built, it is extremely strong to environmental condition adaptability, there is drought resisting, cold-resistant, impoverishment tolerant, saline-alkali tolerant.
Black fruit fructus lycii is also a kind of wild good plant for integrating medicinal, greening and water and soil conservation value, and fruit is rich
Containing multiple nutritional components such as protein, polysaccharides, it is " soft to be known as plant for the significantly larger than common red matrimony vine of medicinal, health value
Gold ".With the further utilization of the species medical value, wild resource destroys serious, population quantity large area reduction,
Even there are the phenomena of mortality in blocks in some areas, which currently has been cited as important protection plant.In this harsh situation
Under, the main task that effectively breeding, protection and research are current black fruit fructus lycii Rational Managements is carried out to black fruit fructus lycii.
At present, black fruit fructus lycii is mainly based on seminal propagation, it is also possible to which branch buries cuttage and root turion breeding, but universal
The problems such as easily morphing there are the new plant that survival rate is low, reproduction speed is slow, seed seedling-raising generates.
Invention content
In view of this, the purpose of the present invention is to provide a kind of survival rate is high, reproduction speed is fast, the black fruit fructus lycii without variation
The method that half Water culture is taken root.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical scheme：A kind of half Water culture of black fruit fructus lycii life
The method of root, includes the following steps：1) concussion processing black fruit fructus lycii 7~9h of branch at 26~30 DEG C, will concussion treated branch
Cuttage branch is treated in acquisition after item is placed in 6~9 DEG C of 0.5~1.5h of low-temperature treatment；2) it is treated described in cuttage branch cutting to matrix
It carries out culture of rootage 40d~50d and obtains field-transplanting seedling；Solution of taking root is poured to matrix in the process of rooting culture, is kept
The mass volume ratio (g/ml) of matrix and solution of taking root is 1：(2~3)；A concentration of 0.001~0.01mg/ of the solution of taking root
L。
Preferably, the concussion processing described in step 1) is left and right cycle concussion processing.
Preferably, the frequency of the concussion processing is 25~35rpm.
Preferably, matrix described in step 2) includes vermiculite and sawdust.
Preferably, the volume ratio of the vermiculite and sawdust is 1：(0.8~1.2).
Preferably, it is 8~10cm that the depth in cuttage branch cutting to matrix is treated described in step 2).
Preferably, the temperature of the culture of rootage is 23~28 DEG C.
Preferably, 2 initial~4d of the culture of rootage is carried out under dark condition；Follow-up culture of rootage is natural indoors
It is carried out under illumination condition.
Preferably, the solution of taking root is indolebutyric acid solution.
Preferably, a concentration of 0.003~0.007mg/L of the indolebutyric acid solution.
Beneficial effects of the present invention：The method that half Water culture of black fruit fructus lycii of the present invention is taken root, using 26~30
Concussion, which is handled, at DEG C coordinates 6~9 DEG C of low-temperature treatment black fruit fructus lycii branches, endogenous growth hormone in induced activation black fruit fructus lycii branch
Activity, cuttage process cooperation external source takes root the use of solution, is conducive to root system fast-growth after cuttage, the black fruit fructus lycii branch
Rooting rate can reach 100% after cuttage, and root growth is healthy and strong, and 40d~50d can obtain field-transplanting seedling；It is of the present invention
The method taken root of half Water culture of black fruit fructus lycii can keep maternal excellent inhereditary feature, overcome seed seed propagation seedling
The problem of speed is slowly and inhereditary feature variation easily occurs for the new plant of gained, method side easy to operate provided by the present invention
Just, growing-seedling period is short, and seedling speed is fast, can be applied to the industrialization breeding of black fruit fructus lycii nursery stock.
Specific embodiment
The present invention provides a kind of methods that half Water culture of black fruit fructus lycii is taken root, and include the following steps：1) 26~30 DEG C
Lower concussion processing black fruit fructus lycii 7~9h of branch, will concussion treated obtains after branch is placed in 6~9 DEG C of 0.5~1.5h of low-temperature treatment
It must treat cuttage branch；2) treat that culture of rootage 40d~50d is carried out in cuttage branch cutting to matrix obtains field-transplanting by described
Seedling；The mass volume ratio (g/ml) of solution of taking root, the matrix and solution of taking root is poured in the process of rooting culture to matrix
It is 1：(2~3), a concentration of 0.001~0.01mg/L of the solution of taking root.
The branch of the previous year growth, autumn and winter may be selected during the black fruit fructus lycii branch spring and summer cuttage in the present invention
Current-year branch may be selected；The black fruit fructus lycii branch is the branch on black fruit fructus lycii health maternal plant, preferably no disease and pests harm,
The healthy branch that no livestock gnaws.In the present invention preferably from life for the year described in more than 5cm or so clips at maternal plant branch
Black fruit fructus lycii branch, in order to avoid maternal plant growth is influenced after trimming.The length of black fruit fructus lycii branch is preferably described in clip of the present invention
18~22cm, more preferably 20cm.Its lower cut is preferably cut into horse by the present invention after black fruit fructus lycii branch described in clip
House detergent stoste is dipped after ungulate rapidly；The effect of the house detergent stoste is fresh-keeping, prevents water loss.This hair
After the black fruit fructus lycii spray of the bright current year life dips house detergent stoste, the black fruit fructus lycii of preferably giving birth to the current year
Spray notch impregnates in water downward, and it is for use to be positioned over the place of giving shade.
The present invention carries out low-temperature treatment acquisition after the concussion of black fruit fructus lycii branch is handled and treats cuttage branch.In the present invention
The temperature of the concussion processing is 26~30 DEG C, preferably 28 DEG C；The time of the concussion processing is 7~9h, preferably
8h.The concussion processing preferably left and right cycle concussion processing in the present invention, the frequency of the left and right concussion processing are preferred
It is 25~35rpm, more preferably 30rpm.Concussion processing is preferably using permanent described in specific implementation process of the present invention
Warm concussion and cultivate case realizes that in the concussion processing procedure, the black fruit fructus lycii branch is preferably laid in isothermal vibration culture
In case, and it is disposed vertically with concussion direction.The present invention sets the concussion of the isothermal vibration incubator in specific implementation process
Pattern is left and right concussion pattern, and the concussion frequency of the isothermal vibration incubator is preferable to provide as 25~35rpm, more preferably
For 30rpm.
The present invention carries out low-temperature treatment acquisition after concussion processing, by black fruit fructus lycii branch and treats cuttage branch.At this
Preferably concussion treated the black fruit fructus lycii branch filter paper or newspaper are wrapped up in invention and squirted by paper, with not shape
Into water droplet preferably；The effect of the filter paper or newspaper package branch is to keep the skin wet in time for branch in the present invention, prevents branch
Item dehydration in low-temperature process.The branch that the filter paper or newspaper wrap up is carried out low-temperature treatment, the low temperature by the present invention
The temperature of processing is 6~9 DEG C, preferably 8 DEG C；The time of the low-temperature treatment is 0.5~1.5h, preferably 1h；At this
Low-temperature treatment described in invention is preferably realized using refrigerator.The concussion processing is with reference to the purpose of low-temperature treatment in the present invention
It is breaking dormancy, induction branch in itself take root by existing endogenous growth hormone, quickening branch.
The present invention after cuttage branch, treats to carry out culture of rootage 40d in cuttage branch cutting to matrix in acquisition by described
~50d obtains field-transplanting seedling.In the present invention, the matrix preferably includes vermiculite and sawdust；The vermiculite and sawdust
Volume ratio is preferably 1：(0.8~1.2), more preferably 1:1.The sawdust was preferably 200 mesh sieve in the present invention
Sawdust.
The preferred matrix after disinfecting of the matrix in the present invention.In the present invention, it is described to disinfect
It is preferably carried out using liquor potassic permanganate, the mass concentration of the liquor potassic permanganate is preferably 0.5%；Have in the present invention
In body implementation process, liquor potassic permanganate is preferably sprayed in matrix the processing that carries out disinfection, the present invention is at the disinfection
After reason, the matrix after described disinfect preferably is placed under sunlight to the 2d that is exposed to the sun so that matrix disinfection is more thorough.
Hole tray is preferably distributed into after the matrix disinfection in the present invention, the present invention is before the packing preferably in cave
Disk lower part pad put filter paper in case pour after matrix expose from the hole of hole tray bottom.The present invention is in specific implementation process, preferably
First by glass bar tilt 45 ° be inserted into matrix in, insertion depth is preferably 8~10cm, and skewer will be treated after then taking out glass bar
Item of transplanting is rapidly inserted into the hole left after glass bar takes out, in order to avoid branch dehydration；Inserting hole using glass bar in the present invention is
In order to avoid treating that cuttage branch causes to damage to branch notch during insertion, later stage calli induction is influenced.
The present invention after cuttage branch is inserted into matrix, carries out described culture of rootage 40d~50d and obtains field-transplanting seedling.
The temperature of the culture of rootage is preferably 23~28 DEG C, more preferably 25 DEG C in the present invention.
2 initial~4d of the culture of rootage is carried out under dark condition in the present invention；Follow-up culture of rootage is indoors
Under the conditions of natural lighting.
Solution of taking root is poured to matrix in the process of rooting culture in the present invention, keeps matrix and the matter for solution of taking root
It is 1 to measure volume ratio (g/ml)：(2~3), preferably 1：4；A concentration of 0.001~0.01mg/L of the solution of taking root, it is more excellent
It is selected as 0.004~0.006mg/L.Solution of taking root described in the present invention is preferably rooting powder solution, naphthalene acid solution or Yin
Diindyl butyric acid solution, more preferably indolebutyric acid solution.The a concentration of of heretofore described indolebutyric acid solution is preferably
0.003~0.007mg/L, more preferably 0.005mg/L.
In specific implementation process of the present invention, the hole tray is preferably 32 hole disks, and every hole is preferred in the hole tray
150g matrix is filled, indolebutyric acid solution usage during pouring per hole tray per hole is preferably 300~450ml.The present invention is follow-up
Ensure to keep enough moisture in process of rooting culture in hole tray, during water shortage, indolebutyric acid solution is preferably poured into support
In disk, moisture can be penetrated into along the aperture of hole tray bottom in matrix.In the present invention, the matrix in the hole tray should not mistake
In humidity, otherwise cause matrix mouldy, influence calli induction.The present invention is in the culture of rootage to 13~17d, branch
Inferior end notch director goes out irregular protrusion i.e. callus；The culture of rootage to 22~28d, the callus is grown into
For white root system, the culture of rootage to 35~40d, the main root length of the branch to 5~8cm；The culture of rootage is to 50d
When, many hairs are grown around main root, the condition for having met crop field open country transplanting with offspring at this time, as field-transplanting seedling.
In the present invention, after obtaining field-transplanting seedling, transplant planting preferably is carried out to big transplanted seedling of acquisition.At this
In invention, the field-transplanting seedling of acquisition can directly carry out crop field open country transplant planting after soil defrosting in 3~April of spring.This
Invention is preferably turned over ground, smooth soil before the transplant planting, later with scoop by offspring from matrix
Go out, notice that scoop apart from the position of 8~10cm of seedling base portion or so, avoids generating injury to seedlings root during lifting.
Seeding row spacing is preferably 50~60cm, more preferably 55cm after heretofore described transplanting.The present invention is in the transplant planting
The same day can carry out crop field flood irrigation, sprinkle profoundly water, later can according to weather condition use Routine Management.
Technical solution provided by the invention is described in detail with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.
Embodiment 1
1st, black fruit fructus lycii branch clip
Branch on summer current year August part clip black fruit fructus lycii health maternal plant, lower cut are cut into the shape of a hoof, later rapidly
Notch is dipped into a small amount of house detergent stoste, to ensure the fresh of branch, interior is then taken back, lower cut is soaked into originally
In water, be positioned over give shade place 2-3h it is for use.
2nd, branch is handled
It pulls the branch filter paper impregnated in tap water out or newspaper wraps up and squirts paper, not formed preferably water droplet, with
Anti- branch dehydration is respectively adopted at 8 DEG C and refrigerates 1h processing branches, because low-temperature treatment energy breaking dormancy, induces branch in itself
Existing endogenous growth hormone accelerates branch later period growth and development.
3rd, nutrient solution type and concentration screening
By by the branch cutting of chilling treatment, in 32 hole disks, per hole, dress crosses the saw of 200 mesh soil sieves by 150g
End and river sand are 1 according to volume ratio：1 mixed-matrix, every group of hole tray pours 9600ml mother liquors every time during pouring.Experiment is with originally
Water, Hoagland nutrient solutions, various concentration ABT, IBA, NAA mother liquor fully poured, using tap water as control, difference battalion
Nutrient solution pours rooting efficiency such as table 1.
1 Different Nutrition liquid of table is poured to branch rooting efficiency
When being poured with tap water and Hoagland nutrient solutions, black fruit fructus lycii branch is not taken root.ABT, IBA, NAA concentration are distinguished
When being handled for 0.01mg/L, 0.005mg/L, 0.001mg/L, branch can take root, wherein using a concentration of 0.005mg/L of IBA
When processing branch rooting rate for 66.52%, be secondly the processing of a concentration of 0.001mg/L, branch during a concentration of 0.01mg/L of IBA
Rooting rate minimum.When handling branch using tri- kinds of ABT and NAA different concentration, branch rooting rate size is followed successively by
0.005mg/L processing ＞ 0.001mg/L processing ＞ 0.01mg/L processing.And branch is most during three kinds of different mother liquor various concentrations processing
Early rootage duration and rooting rate size show as same rule.Therefore, using a concentration of black fruits of 0.005mg/L optimums of IBA
The pouring of half water planting of matrimony vine is taken root, but matrix water penetration is poor after the discovery nutrient solution pouring of cuttage process, and branch has part to rot, because
This should further screen the matrix for being more advantageous to root growth.
4th, Screening matrix
Branch rooting efficiency such as table 2 when a certain matrix is used alone in the case of pouring in same nutrient solution, use is a concentration of
The IBA of 0.005mg/L carries out pouring processing, the matrix species used in cuttage be respectively individually using vermiculite, peat soil, river sand,
Five kinds of perlite, sawdust, the rooting rate of wherein vermiculite and peat soil is higher, and respectively 75.33% and 74.91%, but individually adopt
It is shorter that water spacer number of days is poured during with vermiculite, is 6d, illustrates that vermiculite has higher ventilative, permeability rate, after can guarantee branch cutting
Imputrescibility, perlite also have very high ventilative, permeability rate, but when individually using perlite, and branch rooting rate is too low, unfavorable
In black fruit fructus lycii shoot growth；When individually using sawdust, watering interval time is longer, but rooting rate is relatively low.
Although branch rooting rate is higher when a certain matrix is used alone, nutrient solution pours interval number of days too after cuttage
It is few, the waste of nutrient solution is caused, greatly improves toxigenic capacity.Therefore, the further preferred of cutting medium need to be carried out, reduces life
Nutrient solution pours number during root.
Branch rooting efficiency when 2 same nutrient solution of table pours a kind of matrix
5th, matrix is preferred
Using vermiculite as the basic matrix of branch cutting, by its according to different volume ratios respectively with peat soil, river sand,
Perlite, sawdust are according to 1：1 or 1：2 volume ratio is mixed, and branch is taken root, and the results are shown in Table 3, wherein vermiculite and sawdust
According to 1：Branch rooting rate highest in the matrix that 1 volume ratio mixes, be 88.96%, than be used alone vermiculite when rooting rate
Improve 13.63%, it is 10d that nutrient solution, which pours interval number of days, and than a kind of matrix is used alone when increases 3-6d, other matrix according to
1：1 or 1：The rooting rate of branch is between 45%~75% in 2 volume ratio mixed-matrix, be below vermiculite and sawdust according to
1：Rooting rate when 1 volume ratio mixes.Therefore, vermiculite and sawdust are selected according to 1：The mixed-matrix of 1 volume ratio is as black fruit
The optimal medium matter that half water planting of matrimony vine branch is taken root.
Branch rooting efficiency when 3 same nutrient solution of table pours different substrates
6th, it is managed after cuttage
Since vermiculite and sawdust water penetration are preferable, the aperture of extra liquid runs down hole tray bottom flows into pallet after pouring
In.Just cuttage is complete fully pour water after, pallet is placed into 3d together with the good branch of cuttage under dark condition, later move into interior,
Routine Management is carried out at 25 DEG C of room temperature.Only need to keep enough moisture in final-period management in pallet, during water shortage,
Directly IBA (indolebutyric acid) mother liquor of prepared 0.005mg/L is poured into pallet, moisture can be along the aperture of hole tray bottom
It penetrates into matrix (matrix in hole tray should not be excessively moist, otherwise causes matrix mouldy, influences calli induction).
About 15d or so, branch inferior end notch director go out irregular protrusion i.e. callus；These callus of about 25d or so are gradual
It is grown to white root system, about 38d main roots length to 5~8cm grows many hairs during 50d around main root, and band offspring at this time has been expired
The condition of sufficient crop field open country transplanting.
7th, transplant planting
Open country transplanting was carried out at the beginning of 10 months by the end of September for the year, is turned over ground before transplanting, smooth soil, later with shovel
Son by offspring from matrix go out, notice during lifting that scoop apart from the position of 8~10cm of seedling base portion or so, is avoiding pair
Seedlings root generates injury, and seeding row spacing keeps 50~60cm after transplanting.The transplanting same day carries out crop field flood irrigation, sprinkles profoundly water, it
After can according to weather condition use Routine Management.
Embodiment 2
Explanation：In the present embodiment using the IBA of a concentration of 0.005mg/L preferably gone out as solution of taking root, with vermiculite, sawdust by
According to 1：The matrix that 1 volume ratio mixes is as cutting medium.Wherein branch clip time and training method, management etc. after taking root
It is same as Example 1.
1st, branch cutting pretreatment mode is preferred
It pulls the branch filter paper impregnated in tap water out or newspaper wraps up and squirts paper, not formed preferably water droplet, with
Anti- branch dehydration is respectively adopted at soak at room temperature and 1 DEG C, 8 DEG C, 15 DEG C and impregnates 1h, 3h, 8h processing branch, to be placed under room temperature
It is each to handle situation such as following table of taking root for control.
Influence of the 1 branch cutting pretreatment mode of table to taking root
During using 1 DEG C of refrigeration different time processing branch, it can take root after branch cutting when processing time is 1h, rooting rate
It is 33.06%, and when 1 DEG C of processing time is 3h and 8h, since the processing low-temperature treatment time is longer, part branch is freezed to death, right
It takes root and makes a big impact after cuttage；During using 8 DEG C of processing branches, there are higher rooting rate in three kinds of processing times, wherein, it adopts
During with 8 DEG C of refrigeration 3h, branch 100% can take root, the processing rooting rate of other two cold preservation time sections also 90% or so, and
And rootage duration is more early；When being refrigerated using 15 DEG C, three kinds of processing time branch rooting rates 70% or so, and rootage duration compared with
Evening.Therefore, be conducive to the growth and development of root system before cuttage using 8 DEG C of refrigeration 3h.
By above-described embodiment it is found that the side that half Water culture of black fruit fructus lycii of the present invention is taken root
Method is handled using concussion at 28 DEG C and coordinates 8 DEG C of low-temperature treatment black fruit fructus lycii branches, endogenous life in induced activation black fruit fructus lycii branch
The activity of long hormone, cuttage process coordinate the use of indolebutyric acid solution, are conducive to root system fast-growth after cuttage, the black fruit
Rooting rate can reach 100% after matrimony vine branch cutting, and root growth is healthy and strong；Method side easy to operate provided by the present invention
Just, growing-seedling period is short, and seedling speed is fast, can be applied to the industrialization breeding of black fruit fructus lycii nursery stock.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of method that half Water culture of black fruit fructus lycii is taken root, includes the following steps：
1) concussion processing black fruit fructus lycii 7~9h of branch at 26~30 DEG C, will concussion treated that branch is placed at 6~9 DEG C of low temperature
0.5~1.5h is managed, cuttage branch is treated in acquisition；
2) it treats to carry out culture of rootage 40d~50d in cuttage branch cutting to matrix by described, obtains field-transplanting seedling；The life
It pours and takes root solution into matrix in root incubation, the mass volume ratio (g/ml) of the matrix and solution of taking root is 1：(2~
3)；A concentration of 0.001~0.01mg/L of the solution of taking root.
2. according to the method described in claim 1, it is characterized in that, the concussion processing described in step 1) is left and right cycle concussion
Processing.
3. method according to claim 1 or 2, which is characterized in that the frequency of the concussion processing is 25~35rpm.
4. according to the method described in claim 1, it is characterized in that, matrix described in step 2) includes vermiculite and sawdust.
5. according to the method described in claim 4, it is characterized in that, the volume ratio of the vermiculite and sawdust is 1：(0.8~
1.2)。
6. according to the method described in claim 1,4 or 5, which is characterized in that treat cuttage branch cutting to base described in step 2)
Depth in matter is 8~10cm.
7. according to the method described in claim 1, it is characterized in that, the temperature of the culture of rootage is 23~28 DEG C.
8. the method according to claim 1 or 7, which is characterized in that 2 initial~4d of the culture of rootage is in dark condition
Lower progress；It is carried out under the conditions of follow-up culture of rootage natural lighting indoors.
9. according to the method described in claim 1, it is characterized in that, the solution of taking root is indolebutyric acid solution.
10. according to the method described in claim 9, it is characterized in that, a concentration of the 0.003 of the indolebutyric acid solution~
0.007mg/L。
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